Rroxscaffold_5G00372340

ribosome biogenesis GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010219
Physical Location & Seq
Reverse (-)
53789540 .. 53792626
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372340.1

Sequence Viewer

Length: 1443 bp
ATGCCGATTGCCTCCATCTCAATTCTCCGCCACCGCTCCGTCACCGCCACCGTCACCGTCACTCTCCCCACCTTCAACGACCTCTGCCGCCGATTCTCAACCGTCCGATGCGTCCCTATCGCAGCTGCAAAGCACCCTAACGTCACCAGAAAATCTCAGCCGCCGGACAAGAAGCTCCTCCGAGCTAAACAGACCTTCAAGGACTACTCCTCCCTCGCCCCGGTCCTTTCTCCTCAGGACAAGCCTCCTCTCTCGGAATCCCAGACCGTTGGCACCGTCGCCGCCGCTCAGGCCAACTTCATGCGCGTCATCGTCGAACCTCCTGGAAGCCTCGAGAGGAGCGCGGACGAAACGGCGTCGTCGTCTTCTGGATTTCAGGTGGAGCTGCTGTGTGTGGTGAGGGCGTTGTTGAAGAAGATAAAGAGGAGAGTGTTGGTTGGGGACAAGGTGCTGGTGGGGTCCATTGACTGGGTGGACCGCCGGGGAATGATCGAGAATGTGTACCGGAGGAGCTCCGAGATTCTTGACCCGCCGGTGGCCAATGTGGACCATCTGCTTGTGCTTTTCTCTCTGGAGCAGCCCAAGCTTGACCCCTTCGCGCTCACTAGGTTTCTAATTGAGGCCGAGTCCACTGGAATTCCACTCACTCTGGCGCTCAACAAGTCTGAGCTGGTTGACGAAGAGACAAAGGCTACATGGAAGTCTAGGCTACATAAGTGGGGCTACAAGCCACTATTTTGCAGCGTTGAATCTAAATCTGGACTCGACTCTCTGGCTTTTATTTTGAGAGATCAAACAAGTGTAATTGTGGGTCCAAGCGGAGTTGGAAAGTCTAGTCTGATCAATGCTTTGAGGAATAGCGACCATCATGCTTTTGATGCTGCAGAAGTGGATAATCGGCTTGATCCAATTTCAGGCAGCAAGTGGTTGGAGGATCAGCGTGTTGGAGAGGTTTCAGTAAGAAGTGGTAAAGGGAAGCATACCACTCGCCACGTTTCCTTGCTTCCATTGTCTGGAGGAGGGTATCTTGCTGATACTCCTGGGTTCAGCCAGCCTAGTTTAATGAAAGTAACAAAGCAATCTCTGGCACAAGCTTTCCCCGAGATACAGAAGATACTCAATGATAGTGAAGCTGTGAAATGCGCATTCAGTGACTGCTTGCATTTGGGTGAAAAAGGTTGCGTTGTAGGGACTGACTGGGAAAGATATCCATACTATTTCCAACTTCTTGATGAGATCAGAATCAGAGAGGAATTTCAGTTAAGGACATTTGGAACCAAAAAAGAGGGTGATGTAAGGATCAAGGATGGACAAGCTGAACCAAGGTTGGAGCGTAAGAAGCATAGGAGAGTATCTCGCAAGAGTATGAACCAATCATTACTAGATGAATTAGATGAGCTTGATGACGACGACGACAACTTACTAGATGAGGTGAACCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
RsgA_GTPase

Protein Analysis

480

Amino Acids

53.51

Weight (kDa)

8.52

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsgA_GTPase PF03193 168 - 354 2.8e-47 RsgA GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1144
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 2 cut(s) 468, 1013
AccBSI CCGCTC 2 cut(s) 36, 287
AccII CGCG 3 cut(s) 306, 344, 599
AclWI GGATC 3 cut(s) 899, 942, 1307
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 636, 1253
AcyI GRCGYC 1 cut(s) 356
AfaI GTAC 1 cut(s) 503
AfiI CCNNNNNNNGG 5 cut(s) 220, 468, 535, 914, 1013
AgsI TTSAA 4 cut(s) 76, 199, 412, 749
AjnI CCWGG 2 cut(s) 322, 1039
Alw21I GWGCWC 1 cut(s) 515
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 3 cut(s) 899, 942, 1307
AlwNI CAGNNNCTG 1 cut(s) 1155
Ama87I CYCGRG 2 cut(s) 332, 1100
AoxI GGCC 3 cut(s) 291, 537, 621
ApeKI GCWGC 7 cut(s) 122, 125, 385, 577, 741, 881, 918
ApoI RAATTY 2 cut(s) 636, 1253
AspLEI GCGC 5 cut(s) 306, 344, 601, 655, 1145
AspS9I GGNCC 5 cut(s) 223, 459, 475, 547, 812
AsuC2I CCSGG 2 cut(s) 221, 482
AsuHPI GGTGA 6 cut(s) 34, 46, 136, 409, 1181, 1301
AvaI CYCGRG 2 cut(s) 332, 1100
AvaII GGWCC 5 cut(s) 223, 459, 475, 547, 812
AxyI CCTNAGG 1 cut(s) 234
BalI TGGCCA 1 cut(s) 539
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 515
BbvI GCAGC 7 cut(s) 112, 134, 372, 589, 753, 868, 930
BccI CCATC 4 cut(s) 23, 558, 873, 1301
BceAI ACGGC 1 cut(s) 369
BcgI CGANNNNNNTGC 4 cut(s) 851, 885, 968, 1002
BciT130I CCWGG 2 cut(s) 324, 1041
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 2 cut(s) 221, 482
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 6 cut(s) 606, 705, 834, 1056, 1382, 1424
BfmI CTRYAG 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 290
Bme1390I CCNGG 4 cut(s) 221, 324, 482, 1041
Bme18I GGWCC 5 cut(s) 223, 459, 475, 547, 812
BmeT110I CYCGRG 2 cut(s) 332, 1100
BmgT120I GGNCC 5 cut(s) 223, 459, 475, 547, 812
BmiI GGNNCC 4 cut(s) 274, 460, 813, 1276
BmrFI CCNGG 4 cut(s) 221, 324, 482, 1041
BmrI ACTGGG 2 cut(s) 478, 1207
BmsI GCATC 2 cut(s) 98, 868
BmuI ACTGGG 2 cut(s) 478, 1207
BpiI GAAGAC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 1339, 1371
BpmI CTGGAG 2 cut(s) 593, 1035
Bpu10I CCTNAGC 1 cut(s) 288
BpuMI CCSGG 2 cut(s) 221, 482
BsaBI GATNNNNATC 1 cut(s) 1241
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 219, 481, 1040, 1322
BsaWI WCCGGW 1 cut(s) 504
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 374, 404
Bsc4I CCNNNNNNNGG 5 cut(s) 220, 468, 535, 914, 1013
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 3 cut(s) 473, 637, 1202
Bse21I CCTNAGG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 1241
BseBI CCWGG 2 cut(s) 324, 1041
BseDI CCNNGG 4 cut(s) 219, 481, 1040, 1322
BseGI GGATG 1 cut(s) 1312
BseJI GATNNNNATC 1 cut(s) 1241
BseLI CCNNNNNNNGG 5 cut(s) 220, 468, 535, 914, 1013
BseMII CTCAG 4 cut(s) 170, 248, 302, 657
BseNI ACTGG 3 cut(s) 473, 637, 1202
BseRI GAGGAG 8 cut(s) 167, 199, 222, 237, 352, 439, 523, 1032
BseXI GCAGC 7 cut(s) 112, 134, 372, 589, 753, 868, 930
Bsh1236I CGCG 3 cut(s) 306, 344, 599
BshFI GGCC 3 cut(s) 293, 539, 623
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 515
BsiHKCI CYCGRG 2 cut(s) 332, 1100
BsiSI CCGG 5 cut(s) 164, 221, 481, 505, 533
BslFI GGGAC 3 cut(s) 98, 455, 1204
BslI CCNNNNNNNGG 5 cut(s) 220, 468, 535, 914, 1013
BsmAI GTCTC 1 cut(s) 677
BsmFI GGGAC 3 cut(s) 98, 455, 1204
BsmI GAATGC 1 cut(s) 1145
BsnI GGCC 3 cut(s) 293, 539, 623
BsoBI CYCGRG 2 cut(s) 332, 1100
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
BspANI GGCC 3 cut(s) 293, 539, 623
BspCNI CTCAG 4 cut(s) 169, 247, 301, 658
BspFNI CGCG 3 cut(s) 306, 344, 599
BspLI GGNNCC 4 cut(s) 274, 460, 813, 1276
BspMAI CTGCAG 1 cut(s) 886
BspPI GGATC 3 cut(s) 899, 942, 1307
BspT107I GGYRCC 1 cut(s) 272
BsrBI CCGCTC 2 cut(s) 36, 287
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 3 cut(s) 473, 637, 1202
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 4 cut(s) 219, 481, 1040, 1322
BssMI GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
BssNI GRCGYC 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 1322
Bst2UI CCWGG 2 cut(s) 324, 1041
Bst4CI ACNGT 5 cut(s) 52, 58, 103, 268, 277
Bst6I CTCTTC 1 cut(s) 675
BstACI GRCGYC 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 1052, 1160
BstDEI CTNAG 4 cut(s) 156, 234, 288, 666
BstF5I GGATG 1 cut(s) 1312
BstFNI CGCG 3 cut(s) 306, 344, 599
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 5 cut(s) 306, 344, 601, 655, 1145
BstKTI GATC 7 cut(s) 492, 793, 843, 907, 937, 1239, 1302
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
BstMWI GCNNNNNNNGC 4 cut(s) 290, 583, 878, 1339
BstNI CCWGG 2 cut(s) 324, 1041
BstSCI CCNGG 4 cut(s) 219, 322, 480, 1039
BstSFI CTRYAG 1 cut(s) 882
BstUI CGCG 3 cut(s) 306, 344, 599
BstV1I GCAGC 7 cut(s) 112, 134, 372, 589, 753, 868, 930
BstV2I GAAGAC 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 269
Bsu36I CCTNAGG 1 cut(s) 234
BsuRI GGCC 3 cut(s) 293, 539, 623
BtsCI GGATG 1 cut(s) 1312
BtsIMutI CAGTG 2 cut(s) 630, 1156
Cac8I GCNNGC 2 cut(s) 1052, 1160
CaiI CAGNNNCTG 1 cut(s) 1155
CfoI GCGC 5 cut(s) 306, 344, 601, 655, 1145
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 5 cut(s) 223, 459, 475, 547, 812
CseI GACGC 3 cut(s) 100, 295, 345
Csp6I GTAC 1 cut(s) 502
CviAII CATG 3 cut(s) 301, 696, 869
CviQI GTAC 1 cut(s) 502
DdeI CTNAG 4 cut(s) 156, 234, 288, 666
DpnI GATC 7 cut(s) 491, 792, 842, 906, 936, 1238, 1301
DpnII GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
EciI GGCGGA 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 1322
Eco24I GRGCYC 1 cut(s) 515
Eco32I GATATC 1 cut(s) 1208
Eco47I GGWCC 5 cut(s) 223, 459, 475, 547, 812
Eco53kI GAGCTC 1 cut(s) 513
Eco81I CCTNAGG 1 cut(s) 234
Eco88I CYCGRG 2 cut(s) 332, 1100
EcoICRI GAGCTC 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 322, 1039
EcoRV GATATC 1 cut(s) 1208
EcoT14I CCWWGG 1 cut(s) 1322
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 1322
FaeI CATG 3 cut(s) 304, 699, 872
FaiI YATR 8 cut(s) 302, 697, 714, 870, 981, 1213, 1344, 1367
FaqI GGGAC 3 cut(s) 98, 455, 1204
FatI CATG 3 cut(s) 300, 695, 868
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 1 cut(s) 840
FokI GGATG 1 cut(s) 1319
FriOI GRGCYC 1 cut(s) 515
FspAI RTGCGCAY 1 cut(s) 1144
FspBI CTAG 6 cut(s) 606, 705, 834, 1056, 1382, 1424
FspI TGCGCA 1 cut(s) 1144
GlaI GCGC 5 cut(s) 305, 343, 600, 654, 1144
GsuI CTGGAG 2 cut(s) 593, 1035
HaeII RGCGCY 1 cut(s) 656
HaeIII GGCC 3 cut(s) 293, 539, 623
HapII CCGG 5 cut(s) 164, 221, 481, 505, 533
HgaI GACGC 3 cut(s) 100, 295, 345
HhaI GCGC 5 cut(s) 306, 344, 601, 655, 1145
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 3 cut(s) 304, 699, 872
Hin6I GCGC 5 cut(s) 304, 342, 599, 653, 1143
HinP1I GCGC 5 cut(s) 304, 342, 599, 653, 1143
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 2 cut(s) 584, 1092
HinfI GANTC 8 cut(s) 93, 257, 520, 626, 749, 762, 767, 1242
HpaII CCGG 5 cut(s) 164, 221, 481, 505, 533
HphI GGTGA 6 cut(s) 34, 46, 136, 409, 1181, 1301
Hpy166II GTNNAC 6 cut(s) 475, 502, 547, 630, 676, 1435
Hpy188I TCNGA 8 cut(s) 107, 182, 256, 517, 667, 840, 1241, 1247
Hpy188III TCNNGA 9 cut(s) 236, 334, 369, 493, 524, 572, 759, 1014, 1229
Hpy8I GTNNAC 6 cut(s) 475, 502, 547, 630, 676, 1435
Hpy99I CGWCG 6 cut(s) 281, 317, 361, 364, 1412, 1415
HpyAV CCTTC 3 cut(s) 82, 205, 604
HpyCH4III ACNGT 5 cut(s) 52, 58, 103, 268, 277
HpyCH4IV ACGT 2 cut(s) 141, 993
HpyCH4V TGCA 4 cut(s) 128, 741, 884, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 290, 583, 878, 1339
HpyF3I CTNAG 4 cut(s) 156, 234, 288, 666
HpySE526I ACGT 2 cut(s) 141, 993
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 3 cut(s) 304, 699, 872
HspAI GCGC 5 cut(s) 304, 342, 599, 653, 1143
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
LmnI GCTCC 8 cut(s) 41, 180, 339, 382, 510, 518, 574, 1330
Lsp1109I GCAGC 7 cut(s) 112, 134, 372, 589, 753, 868, 930
LweI GCATC 2 cut(s) 98, 868
MaeI CTAG 6 cut(s) 606, 705, 834, 1056, 1382, 1424
MaeII ACGT 2 cut(s) 141, 993
MaeIII GTNAC 6 cut(s) 40, 52, 58, 142, 1069, 1151
MalI GATC 7 cut(s) 491, 792, 842, 906, 936, 1238, 1301
MbiI CCGCTC 2 cut(s) 36, 287
MboI GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
MboII GAAGA 5 cut(s) 357, 424, 427, 692, 1123
MhlI GDGCHC 1 cut(s) 515
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 7 cut(s) 21, 615, 636, 804, 909, 1253, 1388
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 3 cut(s) 635, 756, 761
MmeI TCCRAC 5 cut(s) 805, 909, 925, 1246, 1308
Mox20I TGGCCA 1 cut(s) 539
MscI TGGCCA 1 cut(s) 539
MseI TTAA 2 cut(s) 1061, 1262
MslI CAYNNNNRTG 1 cut(s) 1167
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 5 cut(s) 164, 221, 481, 505, 533
MspR9I CCNGG 4 cut(s) 221, 324, 482, 1041
Mva1269I GAATGC 1 cut(s) 1145
MvaI CCWGG 2 cut(s) 324, 1041
MvnI CGCG 3 cut(s) 306, 344, 599
MwoI GCNNNNNNNGC 4 cut(s) 290, 583, 878, 1339
NciI CCSGG 2 cut(s) 221, 482
NdeII GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
NlaIII CATG 3 cut(s) 304, 699, 872
NlaIV GGNNCC 4 cut(s) 274, 460, 813, 1276
NmeAIII GCCGAG 1 cut(s) 649
NmuCI GTSAC 5 cut(s) 40, 52, 58, 142, 1151
NsbI TGCGCA 1 cut(s) 1144
PaeR7I CTCGAG 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 359
PctI GAATGC 1 cut(s) 1145
PfeI GAWTC 5 cut(s) 93, 257, 520, 749, 1242
PflMI CCANNNNNTGG 2 cut(s) 468, 1013
PfoI TCCNGGA 1 cut(s) 322
PleI GAGTC 3 cut(s) 634, 756, 761
PpsI GAGTC 3 cut(s) 634, 756, 761
Psp124BI GAGCTC 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 322, 1039
PspGI CCWGG 2 cut(s) 322, 1039
PspN4I GGNNCC 4 cut(s) 274, 460, 813, 1276
PspPI GGNCC 5 cut(s) 223, 459, 475, 547, 812
PstI CTGCAG 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 1155
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 1 cut(s) 503
RsaNI GTAC 1 cut(s) 502
RseI CAYNNNNRTG 1 cut(s) 1167
SacI GAGCTC 1 cut(s) 515
SaqAI TTAA 2 cut(s) 1061, 1262
Sau3AI GATC 7 cut(s) 489, 790, 840, 904, 934, 1236, 1299
Sau96I GGNCC 5 cut(s) 223, 459, 475, 547, 812
SchI GAGTC 3 cut(s) 635, 756, 761
ScrFI CCNGG 4 cut(s) 221, 324, 482, 1041
SduI GDGCHC 1 cut(s) 515
SfaNI GCATC 2 cut(s) 98, 868
SfcI CTRYAG 1 cut(s) 882
Sfr274I CTCGAG 1 cut(s) 332
SgrAI CRCCGGYG 1 cut(s) 532
SinI GGWCC 5 cut(s) 223, 459, 475, 547, 812
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 1167
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 7 cut(s) 21, 615, 636, 804, 909, 1253, 1388
SspMI CTAG 6 cut(s) 606, 705, 834, 1056, 1382, 1424
SstI GAGCTC 1 cut(s) 515
StyD4I CCNGG 4 cut(s) 219, 322, 480, 1039
StyI CCWWGG 1 cut(s) 1322
TaaI ACNGT 5 cut(s) 52, 58, 103, 268, 277
TaiI ACGT 2 cut(s) 144, 996
TaqI TCGA 4 cut(s) 315, 333, 492, 765
TasI AATT 7 cut(s) 21, 615, 636, 804, 909, 1253, 1388
TauI GCSGC 4 cut(s) 90, 163, 284, 287
TfiI GAWTC 5 cut(s) 93, 257, 520, 749, 1242
Tru1I TTAA 2 cut(s) 1061, 1262
Tru9I TTAA 2 cut(s) 1061, 1262
TscAI CASTG 2 cut(s) 637, 1156
TseFI GTSAC 5 cut(s) 40, 52, 58, 142, 1151
TseI GCWGC 7 cut(s) 122, 125, 385, 577, 741, 881, 918
Tsp45I GTSAC 5 cut(s) 40, 52, 58, 142, 1151
TspDTI ATGAA 4 cut(s) 289, 1079, 1382, 1401
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 637, 1156
Van91I CCANNNNNTGG 2 cut(s) 468, 1013
VpaK11BI GGWCC 5 cut(s) 223, 459, 475, 547, 812
XapI RAATTY 2 cut(s) 636, 1253
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XhoI CTCGAG 1 cut(s) 332
XspI CTAG 6 cut(s) 606, 705, 834, 1056, 1382, 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.