RchiOBHm_Chr4g0430601

ribosome biogenesis GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010219
Physical Location & Seq
Reverse (-)
55090503 .. 55093769
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39932

Sequence Viewer

Length: 1434 bp
ATGCCGACTGCCTCCATCTCAATTCTCCGCCACCGCTCCGTCACCGTCACTCTCCCCACCTTCAACGACCTCTGCCGCCGATTCTCAACCGTCCGATGCGTCCCTATCGCAGCAGCAAAGCACCCTAACGTCACCAGAAAATCGCAGCCGCCGGACAAGAAGCTCCTCCGAGCTAAACAGACCTTCAAGGACTACTCCTCCCTCGCCCCGGTCCTCTCTCCTCAGGACAAGCCTCCTCTCTCGGAATCCCAGACCGTCGGCACCGTCGCCGCCGCTCAGGCCAACTTCATGCGCGTCATCGTCGAACCTCCTGGAAGCCTCGAGAAGAGCGCGGACGAAACGGCGTCGTCGTCGTCTTCTGGATTTCAGGTGGAGCTGCTGTGTGTGGTGAGGGCGTTGTTGAAGAAGATAAAGAGGAGAGTGTTGGTTGGGGACAAGGTGCTGGTGGGGTCCATTGACTGGGTGGACCGCCGGGGAATGATCGAGAATGTGTACCGGAGGAGCTCCGAGATTCTTGACCCGCCGGTGGCCAATGTGGACCATCTGCTCGTGCTTTTTTCTCTGGAGCAGCCCAAGCTTGACCCCTTCGCGCTCACTAGGTTTCTGGTTGAGGCCGAGTCCACTGGAATTCCACTCACTCTGGCTCTCAACAAGTCTGAGCTGGTTCACGAAGAGACAAAGGCTACATGGAAGTCTAGGCTACATAAGTGGGGCTACAAGCCACTATTTTGCAGCGTTGAATCTAAATCTGGACTCGACTCTCTTGCTTTTATTTTGAGAGATCAAACAAGTGTAATTGTGGGTCCAAGTGGAGTTGGAAAGTCAAGTCTGATCAATGCTTTGAGGAATAGCGACCATCATGCTTTTGATGCTGCAGAAGTGGATAATCGGCTTGATCCAATTTCAGGCAGCAAGTGGTTGGAGGATCAGCGTGTTGGAGAGGTTTCAGTAAGAAGTGGTAAAGGGAAGCATACCACTCGCCACGTTTCCTTGCTTCCATTGTCTGGAGGAGGGTATCTTGCTGATACTCCTGGGTTCAGCCAGCCTAGTTTAATGAAAGTAACAAAGCAATCTCTGGCACAAGCTTTCCCCGAGATACAGAAGATACTCAATGACAGTGAAGCTGCGAAATGCTCATTCAGTGACTGCTTGCATTTGGGTGAAAAAGGTTGCGTTGTAGGGACTGACTGGGAAAGATACCCGTACTATTTCCAACTTCTTGATGAGATCAGAATCAGAGAGGAATTTCAGTTAAGGACATTTGGAACCAAAAAAGAGGGTGATGTAAGGATCAAGGATGGACAAGCTGAACCAAGGTTGGAGCGTAAGAAGCATAGGAGAGTATCTCGCAAGAGTATGAACCAATCAGTACTAGATGAATTAGATGAGCTTGATGATGAAGACGACGACTTACTAGATGAGGTGAACCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
RsgA_GTPase

Protein Analysis

477

Amino Acids

53.18

Weight (kDa)

8.52

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsgA_GTPase PF03193 165 - 351 2.1e-47 RsgA GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 2 cut(s) 459, 1004
AccBSI CCGCTC 2 cut(s) 36, 275
AccII CGCG 3 cut(s) 294, 332, 590
AciI CCGC 9 cut(s) 28, 34, 76, 149, 270, 273, 332, 469, 521
AclWI GGATC 3 cut(s) 890, 933, 1298
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 2 cut(s) 627, 1244
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 3 cut(s) 494, 1205, 1371
AfiI CCNNNNNNNGG 5 cut(s) 208, 459, 526, 905, 1004
AgsI TTSAA 4 cut(s) 64, 187, 403, 740
AjnI CCWGG 2 cut(s) 310, 1030
Alw21I GWGCWC 1 cut(s) 506
Alw26I GTCTC 1 cut(s) 668
AlwI GGATC 3 cut(s) 890, 933, 1298
AlwNI CAGNNNCTG 1 cut(s) 1146
Ama87I CYCGRG 2 cut(s) 320, 1091
AoxI GGCC 3 cut(s) 279, 528, 612
ApeKI GCWGC 9 cut(s) 110, 113, 145, 376, 568, 732, 872, 909, 1124
ApoI RAATTY 2 cut(s) 627, 1244
AspLEI GCGC 3 cut(s) 294, 332, 592
AspS9I GGNCC 5 cut(s) 211, 450, 466, 538, 803
AsuC2I CCSGG 2 cut(s) 209, 473
AsuHPI GGTGA 5 cut(s) 34, 124, 400, 1172, 1292
AvaI CYCGRG 2 cut(s) 320, 1091
AvaII GGWCC 5 cut(s) 211, 450, 466, 538, 803
AxyI CCTNAGG 1 cut(s) 222
BalI TGGCCA 1 cut(s) 530
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 506
BauI CACGAG 1 cut(s) 548
BbsI GAAGAC 2 cut(s) 348, 1407
Bbv12I GWGCWC 1 cut(s) 506
BbvI GCAGC 9 cut(s) 122, 125, 157, 363, 580, 744, 859, 921, 1111
BccI CCATC 4 cut(s) 23, 549, 864, 1292
BceAI ACGGC 1 cut(s) 357
BcgI CGANNNNNNTGC 6 cut(s) 746, 780, 842, 876, 959, 993
BciT130I CCWGG 2 cut(s) 312, 1032
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 2 cut(s) 209, 473
BcoDI GTCTC 1 cut(s) 668
BfaI CTAG 5 cut(s) 597, 696, 1047, 1373, 1415
BfmI CTRYAG 1 cut(s) 873
BglI GCCNNNNNGGC 1 cut(s) 278
BmcAI AGTACT 1 cut(s) 1371
Bme1390I CCNGG 4 cut(s) 209, 312, 473, 1032
Bme18I GGWCC 5 cut(s) 211, 450, 466, 538, 803
BmeT110I CYCGRG 2 cut(s) 320, 1091
BmgT120I GGNCC 5 cut(s) 211, 450, 466, 538, 803
BmiI GGNNCC 4 cut(s) 262, 451, 804, 1267
BmrFI CCNGG 4 cut(s) 209, 312, 473, 1032
BmrI ACTGGG 2 cut(s) 469, 1198
BmsI GCATC 2 cut(s) 86, 859
BmuI ACTGGG 2 cut(s) 469, 1198
BpiI GAAGAC 2 cut(s) 348, 1407
BplI GAGNNNNNCTC 2 cut(s) 1330, 1362
BpmI CTGGAG 2 cut(s) 584, 1026
Bpu10I CCTNAGC 1 cut(s) 276
BpuMI CCSGG 2 cut(s) 209, 473
BsaBI GATNNNNATC 1 cut(s) 1232
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 4 cut(s) 207, 472, 1031, 1313
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 365, 395
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 459, 526, 905, 1004
Bse118I RCCGGY 1 cut(s) 523
Bse1I ACTGG 3 cut(s) 464, 628, 1193
Bse21I CCTNAGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 1232
BseBI CCWGG 2 cut(s) 312, 1032
BseDI CCNNGG 4 cut(s) 207, 472, 1031, 1313
BseGI GGATG 1 cut(s) 1303
BseJI GATNNNNATC 1 cut(s) 1232
BseLI CCNNNNNNNGG 5 cut(s) 208, 459, 526, 905, 1004
BseMII CTCAG 3 cut(s) 236, 290, 648
BseNI ACTGG 3 cut(s) 464, 628, 1193
BseRI GAGGAG 7 cut(s) 155, 187, 210, 225, 430, 514, 1023
BseXI GCAGC 9 cut(s) 122, 125, 157, 363, 580, 744, 859, 921, 1111
Bsh1236I CGCG 3 cut(s) 294, 332, 590
BshFI GGCC 3 cut(s) 281, 530, 614
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 506
BsiHKCI CYCGRG 2 cut(s) 320, 1091
BsiSI CCGG 5 cut(s) 152, 209, 472, 496, 524
BslFI GGGAC 3 cut(s) 86, 446, 1195
BslI CCNNNNNNNGG 5 cut(s) 208, 459, 526, 905, 1004
BsmAI GTCTC 1 cut(s) 668
BsmFI GGGAC 3 cut(s) 86, 446, 1195
BsnI GGCC 3 cut(s) 281, 530, 614
BsoBI CYCGRG 2 cut(s) 320, 1091
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
BspACI CCGC 9 cut(s) 28, 34, 76, 149, 270, 273, 332, 469, 521
BspANI GGCC 3 cut(s) 281, 530, 614
BspCNI CTCAG 3 cut(s) 235, 289, 649
BspFNI CGCG 3 cut(s) 294, 332, 590
BspLI GGNNCC 4 cut(s) 262, 451, 804, 1267
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 3 cut(s) 890, 933, 1298
BspQI GCTCTTC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 260
BsrBI CCGCTC 2 cut(s) 36, 275
BsrFI RCCGGY 1 cut(s) 523
BsrI ACTGG 3 cut(s) 464, 628, 1193
BssAI RCCGGY 1 cut(s) 523
BssECI CCNNGG 4 cut(s) 207, 472, 1031, 1313
BssMI GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
BssNI GRCGYC 1 cut(s) 344
BssSI CACGAG 1 cut(s) 548
BssT1I CCWWGG 1 cut(s) 1313
Bst2BI CACGAG 1 cut(s) 548
Bst2UI CCWGG 2 cut(s) 312, 1032
Bst4CI ACNGT 5 cut(s) 46, 91, 256, 265, 1118
Bst6I CTCTTC 2 cut(s) 320, 666
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 1043, 1151
BstDEI CTNAG 3 cut(s) 222, 276, 657
BstF5I GGATG 1 cut(s) 1303
BstFNI CGCG 3 cut(s) 294, 332, 590
BstHHI GCGC 3 cut(s) 294, 332, 592
BstKTI GATC 7 cut(s) 483, 784, 834, 898, 928, 1230, 1293
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
BstMWI GCNNNNNNNGC 4 cut(s) 278, 574, 869, 1330
BstNI CCWGG 2 cut(s) 312, 1032
BstSCI CCNGG 4 cut(s) 207, 310, 471, 1030
BstSFI CTRYAG 1 cut(s) 873
BstUI CGCG 3 cut(s) 294, 332, 590
BstV1I GCAGC 9 cut(s) 122, 125, 157, 363, 580, 744, 859, 921, 1111
BstV2I GAAGAC 2 cut(s) 348, 1407
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 3 cut(s) 281, 530, 614
BtsCI GGATG 1 cut(s) 1303
BtsIMutI CAGTG 3 cut(s) 621, 1123, 1147
Cac8I GCNNGC 2 cut(s) 1043, 1151
CaiI CAGNNNCTG 1 cut(s) 1146
CfoI GCGC 3 cut(s) 294, 332, 592
Cfr10I RCCGGY 1 cut(s) 523
Cfr13I GGNCC 5 cut(s) 211, 450, 466, 538, 803
CseI GACGC 3 cut(s) 88, 283, 333
Csp6I GTAC 3 cut(s) 493, 1204, 1370
CviAII CATG 3 cut(s) 289, 687, 860
CviQI GTAC 3 cut(s) 493, 1204, 1370
DdeI CTNAG 3 cut(s) 222, 276, 657
DpnI GATC 7 cut(s) 482, 783, 833, 897, 927, 1229, 1292
DpnII GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 320, 666
EarI CTCTTC 2 cut(s) 320, 666
EciI GGCGGA 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 1313
Eco24I GRGCYC 1 cut(s) 506
Eco47I GGWCC 5 cut(s) 211, 450, 466, 538, 803
Eco53kI GAGCTC 1 cut(s) 504
Eco81I CCTNAGG 1 cut(s) 222
Eco88I CYCGRG 2 cut(s) 320, 1091
EcoICRI GAGCTC 1 cut(s) 504
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 2 cut(s) 310, 1030
EcoT14I CCWWGG 1 cut(s) 1313
EcoT38I GRGCYC 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 1313
FaeI CATG 3 cut(s) 292, 690, 863
FaiI YATR 7 cut(s) 290, 688, 705, 861, 972, 1335, 1358
FaqI GGGAC 3 cut(s) 86, 446, 1195
FatI CATG 3 cut(s) 288, 686, 859
FauI CCCGC 1 cut(s) 528
FbaI TGATCA 1 cut(s) 831
FokI GGATG 1 cut(s) 1310
FriOI GRGCYC 1 cut(s) 506
FspBI CTAG 5 cut(s) 597, 696, 1047, 1373, 1415
GlaI GCGC 3 cut(s) 293, 331, 591
GsuI CTGGAG 2 cut(s) 584, 1026
HaeIII GGCC 3 cut(s) 281, 530, 614
HapII CCGG 5 cut(s) 152, 209, 472, 496, 524
HgaI GACGC 3 cut(s) 88, 283, 333
HhaI GCGC 3 cut(s) 294, 332, 592
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 3 cut(s) 292, 690, 863
Hin6I GCGC 3 cut(s) 292, 330, 590
HinP1I GCGC 3 cut(s) 292, 330, 590
HindIII AAGCTT 2 cut(s) 575, 1083
HinfI GANTC 8 cut(s) 81, 245, 511, 617, 740, 753, 758, 1233
HpaII CCGG 5 cut(s) 152, 209, 472, 496, 524
HphI GGTGA 5 cut(s) 34, 124, 400, 1172, 1292
Hpy166II GTNNAC 6 cut(s) 466, 493, 538, 621, 667, 1426
Hpy188I TCNGA 8 cut(s) 95, 170, 244, 508, 658, 831, 1232, 1238
Hpy8I GTNNAC 6 cut(s) 466, 493, 538, 621, 667, 1426
Hpy99I CGWCG 7 cut(s) 260, 269, 305, 349, 352, 355, 1409
HpyAV CCTTC 3 cut(s) 70, 193, 595
HpyCH4III ACNGT 5 cut(s) 46, 91, 256, 265, 1118
HpyCH4IV ACGT 2 cut(s) 129, 984
HpyCH4V TGCA 3 cut(s) 732, 875, 1153
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 574, 869, 1330
HpyF3I CTNAG 3 cut(s) 222, 276, 657
HpySE526I ACGT 2 cut(s) 129, 984
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 3 cut(s) 292, 690, 863
HspAI GCGC 3 cut(s) 292, 330, 590
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 7 cut(s) 41, 168, 373, 501, 509, 565, 1321
Lsp1109I GCAGC 9 cut(s) 122, 125, 157, 363, 580, 744, 859, 921, 1111
LweI GCATC 2 cut(s) 86, 859
MaeI CTAG 5 cut(s) 597, 696, 1047, 1373, 1415
MaeII ACGT 2 cut(s) 129, 984
MaeIII GTNAC 5 cut(s) 40, 46, 130, 1060, 1142
MalI GATC 7 cut(s) 482, 783, 833, 897, 927, 1229, 1292
MbiI CCGCTC 2 cut(s) 36, 275
MboI GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
MboII GAAGA 7 cut(s) 337, 348, 415, 418, 683, 1114, 1412
MhlI GDGCHC 1 cut(s) 506
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 6 cut(s) 21, 627, 795, 900, 1244, 1379
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 3 cut(s) 626, 747, 752
MmeI TCCRAC 5 cut(s) 796, 900, 916, 1237, 1299
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 2 cut(s) 1052, 1253
MslI CAYNNNNRTG 1 cut(s) 1158
Msp20I TGGCCA 1 cut(s) 530
MspI CCGG 5 cut(s) 152, 209, 472, 496, 524
MspR9I CCNGG 4 cut(s) 209, 312, 473, 1032
MvaI CCWGG 2 cut(s) 312, 1032
MvnI CGCG 3 cut(s) 294, 332, 590
MwoI GCNNNNNNNGC 4 cut(s) 278, 574, 869, 1330
NciI CCSGG 2 cut(s) 209, 473
NdeII GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
NlaIII CATG 3 cut(s) 292, 690, 863
NlaIV GGNNCC 4 cut(s) 262, 451, 804, 1267
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 4 cut(s) 40, 46, 130, 1142
PaeR7I CTCGAG 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PfeI GAWTC 5 cut(s) 81, 245, 511, 740, 1233
PflMI CCANNNNNTGG 2 cut(s) 459, 1004
PfoI TCCNGGA 1 cut(s) 310
PleI GAGTC 3 cut(s) 625, 747, 752
PpsI GAGTC 3 cut(s) 625, 747, 752
Psp124BI GAGCTC 1 cut(s) 506
Psp6I CCWGG 2 cut(s) 310, 1030
PspGI CCWGG 2 cut(s) 310, 1030
PspN4I GGNNCC 4 cut(s) 262, 451, 804, 1267
PspPI GGNCC 5 cut(s) 211, 450, 466, 538, 803
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 1 cut(s) 1146
RsaI GTAC 3 cut(s) 494, 1205, 1371
RsaNI GTAC 3 cut(s) 493, 1204, 1370
RseI CAYNNNNRTG 1 cut(s) 1158
SacI GAGCTC 1 cut(s) 506
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 2 cut(s) 1052, 1253
Sau3AI GATC 7 cut(s) 480, 781, 831, 895, 925, 1227, 1290
Sau96I GGNCC 5 cut(s) 211, 450, 466, 538, 803
ScaI AGTACT 1 cut(s) 1371
SchI GAGTC 3 cut(s) 626, 747, 752
ScrFI CCNGG 4 cut(s) 209, 312, 473, 1032
SduI GDGCHC 1 cut(s) 506
SfaNI GCATC 2 cut(s) 86, 859
SfcI CTRYAG 1 cut(s) 873
Sfr274I CTCGAG 1 cut(s) 320
SgrAI CRCCGGYG 1 cut(s) 523
SinI GGWCC 5 cut(s) 211, 450, 466, 538, 803
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 1158
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 6 cut(s) 21, 627, 795, 900, 1244, 1379
SsiI CCGC 9 cut(s) 28, 34, 76, 149, 270, 273, 332, 469, 521
SspMI CTAG 5 cut(s) 597, 696, 1047, 1373, 1415
SstI GAGCTC 1 cut(s) 506
StyD4I CCNGG 4 cut(s) 207, 310, 471, 1030
StyI CCWWGG 1 cut(s) 1313
TaaI ACNGT 5 cut(s) 46, 91, 256, 265, 1118
TaiI ACGT 2 cut(s) 132, 987
TaqI TCGA 4 cut(s) 303, 321, 483, 756
TasI AATT 6 cut(s) 21, 627, 795, 900, 1244, 1379
TatI WGTACW 1 cut(s) 1369
TauI GCSGC 4 cut(s) 78, 151, 272, 275
TfiI GAWTC 5 cut(s) 81, 245, 511, 740, 1233
Tru1I TTAA 2 cut(s) 1052, 1253
Tru9I TTAA 2 cut(s) 1052, 1253
TscAI CASTG 3 cut(s) 628, 1123, 1147
TseFI GTSAC 4 cut(s) 40, 46, 130, 1142
TseI GCWGC 9 cut(s) 110, 113, 145, 376, 568, 732, 872, 909, 1124
Tsp45I GTSAC 4 cut(s) 40, 46, 130, 1142
TspDTI ATGAA 5 cut(s) 277, 1070, 1373, 1392, 1413
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 628, 1123, 1147
Van91I CCANNNNNTGG 2 cut(s) 459, 1004
VpaK11BI GGWCC 5 cut(s) 211, 450, 466, 538, 803
XapI RAATTY 2 cut(s) 627, 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 460
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 5 cut(s) 597, 696, 1047, 1373, 1415
ZrmI AGTACT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.