Rw4G026080

ribosome biogenesis GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010219
Physical Location & Seq
Reverse (-)
52287582 .. 52290270
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026080.1

Sequence Viewer

Length: 1365 bp
ATGCCGATTGCCTCCATCTCAATTCTCCGCCACCGCTCCGTCACCGTCACTCTCCCCACCTTCAACGTCCTCTGCCGCCGATTCTCAACCGTCCGATGCGTCCCTATCGCAGCAGCAAAGCACCCTAACGTCACCAGAAAATCGCAGCCGCCGGACAAGAAGCTCCTCCGAGCTAAACAGACCTTCAAGGACTACTCCTCCCTCGCCCCGGTCCTCTCTCCTCAGGACAAGCCTCCTCTCTCGGAATCCCAGACCGTCGGCACCGTCGCCGCCGCTCAGGCCAACTTCATGCGCGTCATCGTGGAGCTGCTGTGTGTGGTGAGGGCGTTGTTGAAGAAGATAAAGAGGAGAGTGTTGGTTGGGGACAAGGTGCTGGTGGGGTCCATTGACTGGGTGGACCGCCGGGGAATGATCGAGAATGTGTACCGGAGGAGCTCCGAGATTCTTGACCCGCCGGTGGCCAATGTGGACCATCTGCTCGTGCTTTTCTCTCTGGAGCAGCCCAAGCTTGACCCCTTCGCGCTCACTAGGTTTCTGGTTGAGGCCGAGTCCACTGCAATTCCACTCACTCTGGCTCTCAACAAGTCTGAGCTGGTTGACGAAGAGACAAAGGCTATATGGAAGTCTAGGCTACATAAGTGGGGCTACAAGCCACTATTTTGCAGCGTTGAATCTAAATTTGGACTCGACTCTCTTGCTTTTATTTTGAGAGATCAAACAAGTGTAATTGTGGGTCCAAGTGGAGTTGGAAAGTCTAGTCTGATCAATGCTCTGAGGAACAGCGACCATCATGCTTTTGATGCTGCAGAAGTGGATAATCGGCTTGATCCAATTTCAGGCAGCAAGTGGTTGGAGGATCAGCGTGTTGGAGAGGTTTCAGTAAGAAGTGGTAAAGGGAAGCATACCACTCGCCACGTTTCCTTGCTTCCATTGTCTGGAGGAGGGTATCTTGCTGATACTCCTGGGTTCAGCCAGCCTAGTTTAATGAAAGTAACAAAGCAATCTCTGGCACAAGCTTTCCCCGAGATACAGAAGATACTCAATGACAGTGAAGCTGCGAAATGCTCATTCAGTGACTGCTTGCATTTGGGTGAAAAAGGTTGCGTTGTAGGGACTGACTGGGAAAGATACCCGTACTATTTCCAACTTCTTGATGAGATCAGAATCAGAGAGGAATTTCAGTTAAGGACATTTGGAACCAAAAAAGAGGGTGATGTAAGGATCAAGGATGGACAAGCTGAACCAAGGTTGGAGCGTAAGAAGCATAGGAGAGTATCTCGCAAGAGTATGAACCAATCAGTACTAGATGAATTAGATGAGCTTGATGACGACGACGACGACTTACTAGATGAGGTGAACCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
RsgA_GTPase

Protein Analysis

454

Amino Acids

50.95

Weight (kDa)

8.9

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsgA_GTPase PF03193 142 - 328 2.9e-46 RsgA GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 2 cut(s) 390, 935
AccBSI CCGCTC 2 cut(s) 36, 275
AccII CGCG 2 cut(s) 294, 521
AciI CCGC 8 cut(s) 28, 34, 76, 149, 270, 273, 400, 452
AclWI GGATC 3 cut(s) 821, 864, 1229
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 2 cut(s) 677, 1175
AfaI GTAC 3 cut(s) 425, 1136, 1302
AfiI CCNNNNNNNGG 5 cut(s) 208, 390, 457, 836, 935
AgsI TTSAA 4 cut(s) 64, 187, 334, 671
AjnI CCWGG 1 cut(s) 961
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 3 cut(s) 821, 864, 1229
AlwNI CAGNNNCTG 1 cut(s) 1077
Ama87I CYCGRG 1 cut(s) 1022
AoxI GGCC 3 cut(s) 279, 459, 543
ApeKI GCWGC 9 cut(s) 110, 113, 145, 307, 499, 663, 803, 840, 1055
ApoI RAATTY 2 cut(s) 677, 1175
AspLEI GCGC 2 cut(s) 294, 523
AspS9I GGNCC 5 cut(s) 211, 381, 397, 469, 734
AsuC2I CCSGG 2 cut(s) 209, 404
AsuHPI GGTGA 5 cut(s) 34, 124, 331, 1103, 1223
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 5 cut(s) 211, 381, 397, 469, 734
AxyI CCTNAGG 1 cut(s) 222
BalI TGGCCA 1 cut(s) 461
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 437
BauI CACGAG 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 9 cut(s) 122, 125, 157, 294, 511, 675, 790, 852, 1042
BccI CCATC 4 cut(s) 23, 480, 795, 1223
BcgI CGANNNNNNTGC 8 cut(s) 536, 570, 677, 711, 773, 807, 890, 924
BciT130I CCWGG 1 cut(s) 963
BclI TGATCA 1 cut(s) 762
BcnI CCSGG 2 cut(s) 209, 404
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 6 cut(s) 528, 627, 756, 978, 1304, 1346
BfmI CTRYAG 1 cut(s) 804
BglI GCCNNNNNGGC 1 cut(s) 278
BmcAI AGTACT 1 cut(s) 1302
Bme1390I CCNGG 3 cut(s) 209, 404, 963
Bme18I GGWCC 5 cut(s) 211, 381, 397, 469, 734
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 5 cut(s) 211, 381, 397, 469, 734
BmiI GGNNCC 4 cut(s) 262, 382, 735, 1198
BmrFI CCNGG 3 cut(s) 209, 404, 963
BmrI ACTGGG 2 cut(s) 400, 1129
BmsI GCATC 2 cut(s) 86, 790
BmuI ACTGGG 2 cut(s) 400, 1129
BplI GAGNNNNNCTC 2 cut(s) 1261, 1293
BpmI CTGGAG 2 cut(s) 515, 957
Bpu10I CCTNAGC 1 cut(s) 276
BpuMI CCSGG 2 cut(s) 209, 404
BsaBI GATNNNNATC 1 cut(s) 1163
BsaJI CCNNGG 4 cut(s) 207, 403, 962, 1244
BsaWI WCCGGW 1 cut(s) 426
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 296, 326
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 390, 457, 836, 935
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 2 cut(s) 395, 1124
Bse21I CCTNAGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 1163
BseBI CCWGG 1 cut(s) 963
BseDI CCNNGG 4 cut(s) 207, 403, 962, 1244
BseGI GGATG 1 cut(s) 1234
BseJI GATNNNNATC 1 cut(s) 1163
BseLI CCNNNNNNNGG 5 cut(s) 208, 390, 457, 836, 935
BseMII CTCAG 4 cut(s) 236, 290, 579, 764
BseNI ACTGG 2 cut(s) 395, 1124
BseRI GAGGAG 7 cut(s) 155, 187, 210, 225, 361, 445, 954
BseXI GCAGC 9 cut(s) 122, 125, 157, 294, 511, 675, 790, 852, 1042
Bsh1236I CGCG 2 cut(s) 294, 521
BshFI GGCC 3 cut(s) 281, 461, 545
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 5 cut(s) 152, 209, 403, 427, 455
BslFI GGGAC 3 cut(s) 86, 377, 1126
BslI CCNNNNNNNGG 5 cut(s) 208, 390, 457, 836, 935
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 3 cut(s) 86, 377, 1126
BsnI GGCC 3 cut(s) 281, 461, 545
BsoBI CYCGRG 1 cut(s) 1022
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
BspACI CCGC 8 cut(s) 28, 34, 76, 149, 270, 273, 400, 452
BspANI GGCC 3 cut(s) 281, 461, 545
BspCNI CTCAG 4 cut(s) 235, 289, 580, 765
BspFNI CGCG 2 cut(s) 294, 521
BspLI GGNNCC 4 cut(s) 262, 382, 735, 1198
BspMAI CTGCAG 1 cut(s) 808
BspPI GGATC 3 cut(s) 821, 864, 1229
BspT107I GGYRCC 1 cut(s) 260
BsrBI CCGCTC 2 cut(s) 36, 275
BsrFI RCCGGY 1 cut(s) 454
BsrI ACTGG 2 cut(s) 395, 1124
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 4 cut(s) 207, 403, 962, 1244
BssMI GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 1244
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 963
Bst4CI ACNGT 5 cut(s) 46, 91, 256, 265, 1049
Bst6I CTCTTC 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 974, 1082
BstDEI CTNAG 4 cut(s) 222, 276, 588, 773
BstF5I GGATG 1 cut(s) 1234
BstFNI CGCG 2 cut(s) 294, 521
BstHHI GCGC 2 cut(s) 294, 523
BstKTI GATC 7 cut(s) 414, 715, 765, 829, 859, 1161, 1224
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
BstMWI GCNNNNNNNGC 4 cut(s) 278, 505, 800, 1261
BstNI CCWGG 1 cut(s) 963
BstSCI CCNGG 3 cut(s) 207, 402, 961
BstSFI CTRYAG 1 cut(s) 804
BstUI CGCG 2 cut(s) 294, 521
BstV1I GCAGC 9 cut(s) 122, 125, 157, 294, 511, 675, 790, 852, 1042
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 3 cut(s) 281, 461, 545
BtsCI GGATG 1 cut(s) 1234
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 3 cut(s) 552, 1054, 1078
Cac8I GCNNGC 2 cut(s) 974, 1082
CaiI CAGNNNCTG 1 cut(s) 1077
CfoI GCGC 2 cut(s) 294, 523
Cfr10I RCCGGY 1 cut(s) 454
Cfr13I GGNCC 5 cut(s) 211, 381, 397, 469, 734
CseI GACGC 2 cut(s) 88, 283
Csp6I GTAC 3 cut(s) 424, 1135, 1301
CviAII CATG 2 cut(s) 289, 791
CviQI GTAC 3 cut(s) 424, 1135, 1301
DdeI CTNAG 4 cut(s) 222, 276, 588, 773
DpnI GATC 7 cut(s) 413, 714, 764, 828, 858, 1160, 1223
DpnII GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
EciI GGCGGA 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 1244
Eco24I GRGCYC 1 cut(s) 437
Eco47I GGWCC 5 cut(s) 211, 381, 397, 469, 734
Eco53kI GAGCTC 1 cut(s) 435
Eco81I CCTNAGG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 1244
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 1244
FaeI CATG 2 cut(s) 292, 794
FaiI YATR 8 cut(s) 290, 617, 619, 636, 792, 903, 1266, 1289
FaqI GGGAC 3 cut(s) 86, 377, 1126
FatI CATG 2 cut(s) 288, 790
FauI CCCGC 1 cut(s) 459
FbaI TGATCA 1 cut(s) 762
FokI GGATG 1 cut(s) 1241
FriOI GRGCYC 1 cut(s) 437
FspBI CTAG 6 cut(s) 528, 627, 756, 978, 1304, 1346
GlaI GCGC 2 cut(s) 293, 522
GsuI CTGGAG 2 cut(s) 515, 957
HaeIII GGCC 3 cut(s) 281, 461, 545
HapII CCGG 5 cut(s) 152, 209, 403, 427, 455
HgaI GACGC 2 cut(s) 88, 283
HhaI GCGC 2 cut(s) 294, 523
Hin1II CATG 2 cut(s) 292, 794
Hin6I GCGC 2 cut(s) 292, 521
HinP1I GCGC 2 cut(s) 292, 521
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 2 cut(s) 506, 1014
HinfI GANTC 8 cut(s) 81, 245, 442, 548, 671, 684, 689, 1164
HpaII CCGG 5 cut(s) 152, 209, 403, 427, 455
HphI GGTGA 5 cut(s) 34, 124, 331, 1103, 1223
Hpy166II GTNNAC 6 cut(s) 397, 424, 469, 552, 598, 1357
Hpy188I TCNGA 9 cut(s) 95, 170, 244, 439, 589, 762, 774, 1163, 1169
Hpy188III TCNNGA 6 cut(s) 224, 415, 446, 494, 936, 1151
Hpy8I GTNNAC 6 cut(s) 397, 424, 469, 552, 598, 1357
Hpy99I CGWCG 5 cut(s) 260, 269, 1334, 1337, 1340
HpyAV CCTTC 3 cut(s) 70, 193, 526
HpyCH4III ACNGT 5 cut(s) 46, 91, 256, 265, 1049
HpyCH4IV ACGT 3 cut(s) 66, 129, 915
HpyCH4V TGCA 4 cut(s) 557, 663, 806, 1084
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 505, 800, 1261
HpyF3I CTNAG 4 cut(s) 222, 276, 588, 773
HpySE526I ACGT 3 cut(s) 66, 129, 915
Hsp92II CATG 2 cut(s) 292, 794
HspAI GCGC 2 cut(s) 292, 521
Ksp22I TGATCA 1 cut(s) 762
Kzo9I GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
LmnI GCTCC 7 cut(s) 41, 168, 304, 432, 440, 496, 1252
Lsp1109I GCAGC 9 cut(s) 122, 125, 157, 294, 511, 675, 790, 852, 1042
LweI GCATC 2 cut(s) 86, 790
MaeI CTAG 6 cut(s) 528, 627, 756, 978, 1304, 1346
MaeII ACGT 3 cut(s) 66, 129, 915
MaeIII GTNAC 5 cut(s) 40, 46, 130, 991, 1073
MalI GATC 7 cut(s) 413, 714, 764, 828, 858, 1160, 1223
MbiI CCGCTC 2 cut(s) 36, 275
MboI GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
MboII GAAGA 4 cut(s) 346, 349, 614, 1045
MhlI GDGCHC 1 cut(s) 437
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 7 cut(s) 21, 558, 677, 726, 831, 1175, 1310
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 3 cut(s) 557, 678, 683
MmeI TCCRAC 5 cut(s) 727, 831, 847, 1168, 1230
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 2 cut(s) 983, 1184
MslI CAYNNNNRTG 1 cut(s) 1089
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 5 cut(s) 152, 209, 403, 427, 455
MspR9I CCNGG 3 cut(s) 209, 404, 963
MvaI CCWGG 1 cut(s) 963
MvnI CGCG 2 cut(s) 294, 521
MwoI GCNNNNNNNGC 4 cut(s) 278, 505, 800, 1261
NciI CCSGG 2 cut(s) 209, 404
NdeII GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
NlaIII CATG 2 cut(s) 292, 794
NlaIV GGNNCC 4 cut(s) 262, 382, 735, 1198
NmeAIII GCCGAG 1 cut(s) 571
NmuCI GTSAC 4 cut(s) 40, 46, 130, 1073
PcsI WCGNNNNNNNCGW 1 cut(s) 1335
PfeI GAWTC 5 cut(s) 81, 245, 442, 671, 1164
PflMI CCANNNNNTGG 2 cut(s) 390, 935
PleI GAGTC 3 cut(s) 556, 678, 683
PpsI GAGTC 3 cut(s) 556, 678, 683
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 961
PspGI CCWGG 1 cut(s) 961
PspN4I GGNNCC 4 cut(s) 262, 382, 735, 1198
PspPI GGNCC 5 cut(s) 211, 381, 397, 469, 734
PstI CTGCAG 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 1077
RsaI GTAC 3 cut(s) 425, 1136, 1302
RsaNI GTAC 3 cut(s) 424, 1135, 1301
RseI CAYNNNNRTG 1 cut(s) 1089
SacI GAGCTC 1 cut(s) 437
SaqAI TTAA 2 cut(s) 983, 1184
Sau3AI GATC 7 cut(s) 411, 712, 762, 826, 856, 1158, 1221
Sau96I GGNCC 5 cut(s) 211, 381, 397, 469, 734
ScaI AGTACT 1 cut(s) 1302
SchI GAGTC 3 cut(s) 557, 678, 683
ScrFI CCNGG 3 cut(s) 209, 404, 963
SduI GDGCHC 1 cut(s) 437
SfaNI GCATC 2 cut(s) 86, 790
SfcI CTRYAG 1 cut(s) 804
SgrAI CRCCGGYG 1 cut(s) 454
SinI GGWCC 5 cut(s) 211, 381, 397, 469, 734
SmiMI CAYNNNNRTG 1 cut(s) 1089
Sse9I AATT 7 cut(s) 21, 558, 677, 726, 831, 1175, 1310
SsiI CCGC 8 cut(s) 28, 34, 76, 149, 270, 273, 400, 452
SspMI CTAG 6 cut(s) 528, 627, 756, 978, 1304, 1346
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 207, 402, 961
StyI CCWWGG 1 cut(s) 1244
TaaI ACNGT 5 cut(s) 46, 91, 256, 265, 1049
TaiI ACGT 3 cut(s) 69, 132, 918
TaqI TCGA 2 cut(s) 414, 687
TasI AATT 7 cut(s) 21, 558, 677, 726, 831, 1175, 1310
TatI WGTACW 1 cut(s) 1300
TauI GCSGC 4 cut(s) 78, 151, 272, 275
TfiI GAWTC 5 cut(s) 81, 245, 442, 671, 1164
Tru1I TTAA 2 cut(s) 983, 1184
Tru9I TTAA 2 cut(s) 983, 1184
TscAI CASTG 3 cut(s) 559, 1054, 1078
TseFI GTSAC 4 cut(s) 40, 46, 130, 1073
TseI GCWGC 9 cut(s) 110, 113, 145, 307, 499, 663, 803, 840, 1055
Tsp45I GTSAC 4 cut(s) 40, 46, 130, 1073
TspDTI ATGAA 4 cut(s) 277, 1001, 1304, 1323
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 559, 1054, 1078
Van91I CCANNNNNTGG 2 cut(s) 390, 935
VpaK11BI GGWCC 5 cut(s) 211, 381, 397, 469, 734
XapI RAATTY 2 cut(s) 677, 1175
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XspI CTAG 6 cut(s) 528, 627, 756, 978, 1304, 1346
ZrmI AGTACT 1 cut(s) 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.