MD13G1139300.v1.1

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013694
Physical Location & Seq
Reverse (-)
10726983 .. 10729002
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1139300.v1.1.491

Sequence Viewer

Length: 1971 bp
ATGACTTCGCTCTTAGTAAATCATCAATACACCCCACCCTCCCTGAGCTCGCCCGGGATCCGCGTTTCACCGTTACGCCGCCTTGAATCAAGCTCGACTATGGTGGAGCTCAAGCAGTTGCACTCCCAAGTCATGAGGCTCGGCCTCGCCGCTGACAACGAAGCCATGGGATGTGTCGTCAAGTTTTGCGCCCTCTCCAAAAATGGCGATTTGGGTTATGCCCTCCAGATGTTCGACACAATGCCTCAACCGGATGCATTCATTTACAACACCGTAATGAGAGGCTACTTGCAATGCCAATTGCCGAGAGATTGCATTGTTTTGTACTCACGGATGTTGCAGGACTCTGTCCCTCCCAATCGGTACACGTTCCCTTCCATTGTGCGAGCTTGTTGCGCTGATGGTGCTGTTGGAGAAGGGAAGCAAGTCCATGCCCATGTCGTCAAACTTGGGTTTGGAGATGATGGGTTTTGTCAGAACAATTTGATTCACATGTATGTGAAGTTTCAGTCTTTGGAGGAAGCTAGAAGGGTGTTTGACAAGATGCCCCAGGATGTTGTGTCTTGGACGACTTTGATTACTGGGTACTCCCAATGTGGCTTCATTGATGAGGCTTTTGAAATTTTCGAGTTGATGCCTGAGAAGAATTCTGTTTCTTGGAATGCCATGTTTTCCTCTTATGTCCGGAGTGATCGTTTTCACGAGGCGTTTGCTTTGTTCCAAAGAATGCGAGTGGAAAATGTAAAAGGTCGTACCAAGTGCACAAGGCTCCCGCTTTACGCAGGGTCTGGGAGAGGTGAATGTCGGCAGCCTTACCCCCATTTTATGGAGAGGCTGCTCCCAAGTCTCGAACCCGAGACCTACCGCTCATGGGCGAGTGGAAAATGTTTAGGTGCTCTAGAACAAGGGAAGTGGATACATGGATATATTGAGAAAAGCGGGATTGAATTGGACTCGAAACTTGCAACAATGATCATTGACATGTACTGCAAATGTGGTTGCCTGGAGAAGGCTTTTGAAGTTTTTAACGGGTTGCCAGATAAAGGGATTTCTTCATGGAATTGCATGATTGGTGGGCTAGCAATGCATGGGAAGGGAGAGGCCGCCATTAAACTTTTCGAGCAGATGCAAAGGGATATGGTAGCACCTGACAACATTACTTTTGTTAATGTTCTTAGTGCATGTGCTCACTCAGGGTTAGTAGAAAAGGGGCAGCAGTACTTCCGCAGCATGGTTGAAGTCCACGGTATTGAGCCCCGAACAGAGCATTTTGGCTGCATGGTTGATCTTCTTGGAAGAGCCGGGAGGCTGGAGGAAGCAAGAAAGCTAATAAACGAGATGCCAATGAGCCCCGATGTAGGTGTATTGGGTGCTCTCCTCGGAGCTTGTAAGATCCATGGAAATGTTGAACTGGGAGAGGAAATAGGGAGGAGGGTAATCAAACTGGAAGCAGAAAATAGCGGGCGCTATGTGTTGTTTGCAAATCTATATGCGAATGCGGACAGGTGGGAAGATGTTGCCAATGTGAGAAGATTGATGAATGACAGGGTAGTGAAGAAGGTTCCTGGATTTTCCATGATTGAACTGGAAGGCTCTGTCAATGAATTTATTGCCGGCGGAGGGTCTCATCCTCAGACAAAAGAGATATATGCGAAAGTCGATGAAATGTTGCAATCTATAAGATCTGCAGGATATGTTCCGGACACAGAGGGAGTGTTGCATGATCTTGATGAGGAGGAAAAGAAGAATCCTTTATACTACCACAGTGAGAAACTTGCAATTGCCTTCGGCCTATTGAAGACCAAACCCGGGGAAACTGTTCGTATATCAAAGAATTTACGAGTCTGCAAAGACTGCCACCGGGCAAGCAAGCTCATCTCGAAGGTTTTCCACCGCGAAATAATCGTGAGGGATAGAAATCGCTTCCACCATTTTAGAAGGGGATACTGTTCTTGTAAAGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

657

Amino Acids

74.09

Weight (kDa)

7.95

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 84 - 132 3.7e-10 PPR repeat family
PPR_2 PF13041 185 - 216 4.7e-06 PPR repeat family
PPR_1 PF12854 185 - 212 3e-06 PPR repeat
PPR PF01535 187 - 216 2.9e-08 PPR repeat
PPR_2 PF13041 215 - 249 4.3e-06 PPR repeat family
PPR PF01535 218 - 247 9.7e-07 PPR repeat
PPR_2 PF13041 350 - 397 3.3e-11 PPR repeat family
PPR PF01535 352 - 379 1.1e-06 PPR repeat
E_motif PF20431 466 - 528 5.2e-17 E motif
DYW_deaminase PF14432 564 - 656 1.8e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 826
AccBSI CCGCTC 1 cut(s) 867
AccII CGCG 2 cut(s) 63, 1896
AccIII TCCGGA 2 cut(s) 684, 1699
AclWI GGATC 3 cut(s) 52, 65, 1387
AcsI RAATTY 4 cut(s) 621, 646, 1604, 1834
AfaI GTAC 6 cut(s) 326, 365, 587, 754, 986, 1220
AfiI CCNNNNNNNGG 8 cut(s) 826, 871, 1009, 1043, 1231, 1358, 1620, 1809
AflIII ACRYGT 3 cut(s) 366, 492, 981
AgsI TTSAA 8 cut(s) 86, 620, 947, 1019, 1238, 1409, 1583, 1798
AjnI CCWGG 3 cut(s) 549, 1002, 1564
AluBI AGCT 8 cut(s) 48, 93, 109, 389, 524, 1327, 1385, 1873
AluI AGCT 8 cut(s) 48, 93, 109, 389, 524, 1327, 1385, 1873
Alw21I GWGCWC 6 cut(s) 50, 111, 764, 898, 1189, 1375
Alw26I GTCTC 3 cut(s) 851, 851, 1629
Alw44I GTGCAC 1 cut(s) 760
AlwI GGATC 3 cut(s) 52, 65, 1387
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 3 cut(s) 53, 854, 1808
Aor13HI TCCGGA 2 cut(s) 684, 1699
AoxI GGCC 3 cut(s) 142, 1101, 1789
ApaLI GTGCAC 1 cut(s) 760
ApeKI GCWGC 5 cut(s) 808, 835, 1213, 1227, 1275
ApoI RAATTY 4 cut(s) 621, 646, 1604, 1834
Asp700I GAANNNNTTC 2 cut(s) 1818, 1886
AspLEI GCGC 3 cut(s) 191, 398, 1467
AsuC2I CCSGG 6 cut(s) 54, 55, 1303, 1809, 1810, 1862
AsuHPI GGTGA 2 cut(s) 60, 809
AsuNHI GCTAGC 1 cut(s) 1078
AvaI CYCGRG 3 cut(s) 53, 854, 1808
BaeGI GKGCMC 1 cut(s) 764
BamHI GGATCC 1 cut(s) 57
BanII GRGCYC 4 cut(s) 50, 111, 1257, 1352
BauI CACGAG 1 cut(s) 701
BbsI GAAGAC 1 cut(s) 1805
Bbv12I GWGCWC 6 cut(s) 50, 111, 764, 898, 1189, 1375
BbvI GCAGC 5 cut(s) 820, 822, 1225, 1239, 1262
BccI CCATC 2 cut(s) 395, 458
BcgI CGANNNNNNTGC 4 cut(s) 375, 409, 692, 726
BciT130I CCWGG 3 cut(s) 551, 1004, 1566
BciVI GTATCC 2 cut(s) 909, 1937
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 6 cut(s) 54, 55, 1303, 1809, 1810, 1862
BcoDI GTCTC 3 cut(s) 851, 851, 1629
BfaI CTAG 3 cut(s) 525, 899, 1079
BfmI CTRYAG 1 cut(s) 1686
BfoI RGCGCY 1 cut(s) 1468
BfuI GTATCC 2 cut(s) 909, 1937
BglII AGATCT 1 cut(s) 1682
BisI GCNGC 8 cut(s) 79, 150, 809, 836, 1104, 1214, 1228, 1276
BlsI GCNGC 8 cut(s) 80, 151, 810, 837, 1105, 1215, 1229, 1277
BmcAI AGTACT 1 cut(s) 1220
Bme1390I CCNGG 9 cut(s) 54, 55, 551, 1004, 1303, 1566, 1809, 1810, 1862
BmeT110I CYCGRG 3 cut(s) 53, 854, 1808
BmiI GGNNCC 3 cut(s) 59, 770, 1563
BmrFI CCNGG 9 cut(s) 54, 55, 551, 1004, 1303, 1566, 1809, 1810, 1862
BmrI ACTGGG 2 cut(s) 591, 1421
BmsI GCATC 5 cut(s) 244, 534, 624, 1116, 1329
BmtI GCTAGC 1 cut(s) 1082
BmuI ACTGGG 2 cut(s) 591, 1421
BpiI GAAGAC 1 cut(s) 1805
BpmI CTGGAG 3 cut(s) 209, 1025, 1331
Bpu10I CCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 6 cut(s) 54, 55, 1303, 1809, 1810, 1862
BsaBI GATNNNNATC 1 cut(s) 1917
BsaI GGTCTC 2 cut(s) 851, 1629
BsaJI CCNNGG 8 cut(s) 53, 165, 549, 1243, 1378, 1396, 1808, 1809
BsaWI WCCGGW 3 cut(s) 250, 684, 1699
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bsc4I CCNNNNNNNGG 8 cut(s) 826, 871, 1009, 1043, 1231, 1358, 1620, 1809
Bse118I RCCGGY 1 cut(s) 1613
Bse1I ACTGG 5 cut(s) 586, 1416, 1449, 1590, 1970
Bse3DI GCAATG 2 cut(s) 299, 1089
Bse8I GATNNNNATC 1 cut(s) 1917
BseAI TCCGGA 2 cut(s) 684, 1699
BseBI CCWGG 3 cut(s) 551, 1004, 1566
BseDI CCNNGG 8 cut(s) 53, 165, 549, 1243, 1378, 1396, 1808, 1809
BseGI GGATG 5 cut(s) 176, 259, 339, 559, 1627
BseJI GATNNNNATC 1 cut(s) 1917
BseLI CCNNNNNNNGG 8 cut(s) 826, 871, 1009, 1043, 1231, 1358, 1620, 1809
BseMI GCAATG 2 cut(s) 299, 1089
BseMII CTCAG 4 cut(s) 35, 630, 1206, 1646
BseNI ACTGG 5 cut(s) 586, 1416, 1449, 1590, 1970
BseRI GAGGAG 3 cut(s) 1367, 1444, 1748
BseSI GKGCMC 1 cut(s) 764
BseXI GCAGC 5 cut(s) 820, 822, 1225, 1239, 1262
Bsh1236I CGCG 2 cut(s) 63, 1896
BshFI GGCC 3 cut(s) 144, 1103, 1791
BsiHKAI GWGCWC 6 cut(s) 50, 111, 764, 898, 1189, 1375
BsiHKCI CYCGRG 3 cut(s) 53, 854, 1808
BsiSI CCGG 8 cut(s) 54, 251, 685, 1302, 1614, 1700, 1809, 1861
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 8 cut(s) 826, 871, 1009, 1043, 1231, 1358, 1620, 1809
BsmAI GTCTC 3 cut(s) 851, 851, 1629
BsmFI GGGAC 1 cut(s) 335
BsmI GAATGC 4 cut(s) 257, 667, 732, 1501
BsnI GGCC 3 cut(s) 144, 1103, 1791
Bso31I GGTCTC 2 cut(s) 851, 1629
BsoBI CYCGRG 3 cut(s) 53, 854, 1808
Bsp1286I GDGCHC 8 cut(s) 50, 111, 764, 898, 1189, 1257, 1352, 1375
Bsp13I TCCGGA 2 cut(s) 684, 1699
Bsp143I GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
Bsp19I CCATGG 2 cut(s) 165, 1396
BspANI GGCC 3 cut(s) 144, 1103, 1791
BspCNI CTCAG 4 cut(s) 36, 631, 1205, 1645
BspEI TCCGGA 2 cut(s) 684, 1699
BspFNI CGCG 2 cut(s) 63, 1896
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 3 cut(s) 59, 770, 1563
BspMAI CTGCAG 1 cut(s) 1690
BspOI GCTAGC 1 cut(s) 1082
BspPI GGATC 3 cut(s) 52, 65, 1387
BspQI GCTCTTC 1 cut(s) 1291
BspTNI GGTCTC 2 cut(s) 851, 1629
BsrBI CCGCTC 1 cut(s) 867
BsrDI GCAATG 2 cut(s) 299, 1089
BsrFI RCCGGY 1 cut(s) 1613
BsrI ACTGG 5 cut(s) 586, 1416, 1449, 1590, 1970
BssAI RCCGGY 1 cut(s) 1613
BssECI CCNNGG 8 cut(s) 53, 165, 549, 1243, 1378, 1396, 1808, 1809
BssMI GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
BssSI CACGAG 1 cut(s) 701
BssT1I CCWWGG 2 cut(s) 165, 1396
Bst2BI CACGAG 1 cut(s) 701
Bst2UI CCWGG 3 cut(s) 551, 1004, 1566
Bst4CI ACNGT 6 cut(s) 72, 274, 1247, 1766, 1819, 1949
Bst6I CTCTTC 1 cut(s) 1291
BstAPI GCANNNNNTGC 1 cut(s) 1854
BstC8I GCNNGC 7 cut(s) 50, 387, 1080, 1463, 1615, 1867, 1871
BstDEI CTNAG 6 cut(s) 13, 44, 639, 1175, 1192, 1632
BstDSI CCRYGG 3 cut(s) 165, 1243, 1396
BstENI CCTNNNNNAGG 1 cut(s) 1007
BstF5I GGATG 5 cut(s) 176, 259, 339, 559, 1627
BstFNI CGCG 2 cut(s) 63, 1896
BstH2I RGCGCY 1 cut(s) 1468
BstHHI GCGC 3 cut(s) 191, 398, 1467
BstKTI GATC 7 cut(s) 60, 694, 975, 1288, 1395, 1685, 1726
BstMAI GTCTC 3 cut(s) 851, 851, 1629
BstMBI GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
BstMWI GCNNNNNNNGC 4 cut(s) 395, 404, 1084, 1854
BstNI CCWGG 3 cut(s) 551, 1004, 1566
BstNSI RCATGY 3 cut(s) 496, 985, 1185
BstSCI CCNGG 9 cut(s) 52, 53, 549, 1002, 1301, 1564, 1807, 1808, 1860
BstSFI CTRYAG 1 cut(s) 1686
BstSLI GKGCMC 1 cut(s) 764
BstUI CGCG 2 cut(s) 63, 1896
BstV1I GCAGC 5 cut(s) 820, 822, 1225, 1239, 1262
BstV2I GAAGAC 1 cut(s) 1805
BstX2I RGATCY 3 cut(s) 57, 1392, 1682
BstYI RGATCY 3 cut(s) 57, 1392, 1682
BsuI GTATCC 2 cut(s) 909, 1937
BsuRI GGCC 3 cut(s) 144, 1103, 1791
BtgI CCRYGG 3 cut(s) 165, 1243, 1396
BtsCI GGATG 5 cut(s) 176, 259, 339, 559, 1627
BtsIMutI CAGTG 1 cut(s) 1771
Cac8I GCNNGC 7 cut(s) 50, 387, 1080, 1463, 1615, 1867, 1871
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 1 cut(s) 132
CfoI GCGC 3 cut(s) 191, 398, 1467
Cfr10I RCCGGY 1 cut(s) 1613
Cfr9I CCCGGG 2 cut(s) 53, 1808
Csp6I GTAC 6 cut(s) 325, 364, 586, 753, 985, 1219
CspCI CAANNNNNGTGG 2 cut(s) 893, 928
CviQI GTAC 6 cut(s) 325, 364, 586, 753, 985, 1219
DdeI CTNAG 6 cut(s) 13, 44, 639, 1175, 1192, 1632
DpnI GATC 7 cut(s) 59, 693, 974, 1287, 1394, 1684, 1725
DpnII GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
Eam1104I CTCTTC 1 cut(s) 1291
EarI CTCTTC 1 cut(s) 1291
EciI GGCGGA 1 cut(s) 1632
Ecl136II GAGCTC 2 cut(s) 48, 109
Eco130I CCWWGG 2 cut(s) 165, 1396
Eco24I GRGCYC 4 cut(s) 50, 111, 1257, 1352
Eco31I GGTCTC 2 cut(s) 851, 1629
Eco53kI GAGCTC 2 cut(s) 48, 109
Eco88I CYCGRG 3 cut(s) 53, 854, 1808
EcoICRI GAGCTC 2 cut(s) 48, 109
EcoNI CCTNNNNNAGG 1 cut(s) 1007
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 549, 1002, 1564
EcoT14I CCWWGG 2 cut(s) 165, 1396
EcoT22I ATGCAT 2 cut(s) 259, 1089
EcoT38I GRGCYC 4 cut(s) 50, 111, 1257, 1352
ErhI CCWWGG 2 cut(s) 165, 1396
FaqI GGGAC 1 cut(s) 335
FauI CCCGC 3 cut(s) 780, 932, 1454
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 8 cut(s) 79, 150, 809, 836, 1104, 1214, 1228, 1276
FokI GGATG 5 cut(s) 183, 266, 346, 566, 1614
FriOI GRGCYC 4 cut(s) 50, 111, 1257, 1352
Fsp4HI GCNGC 8 cut(s) 79, 150, 809, 836, 1104, 1214, 1228, 1276
FspBI CTAG 3 cut(s) 525, 899, 1079
GlaI GCGC 3 cut(s) 190, 397, 1466
GluI GCNGC 8 cut(s) 79, 150, 809, 836, 1104, 1214, 1228, 1276
GsuI CTGGAG 3 cut(s) 209, 1025, 1331
HaeII RGCGCY 1 cut(s) 1468
HaeIII GGCC 3 cut(s) 144, 1103, 1791
HapII CCGG 8 cut(s) 54, 251, 685, 1302, 1614, 1700, 1809, 1861
HhaI GCGC 3 cut(s) 191, 398, 1467
Hin6I GCGC 3 cut(s) 189, 396, 1465
HinP1I GCGC 3 cut(s) 189, 396, 1465
HinfI GANTC 6 cut(s) 86, 344, 487, 953, 1747, 1842
HpaII CCGG 8 cut(s) 54, 251, 685, 1302, 1614, 1700, 1809, 1861
HphI GGTGA 2 cut(s) 60, 809
Hpy166II GTNNAC 3 cut(s) 366, 762, 1243
Hpy188I TCNGA 3 cut(s) 477, 1382, 1635
Hpy8I GTNNAC 3 cut(s) 366, 762, 1243
HpyCH4III ACNGT 6 cut(s) 72, 274, 1247, 1766, 1819, 1949
HpyCH4IV ACGT 1 cut(s) 368
HpyF10VI GCNNNNNNNGC 4 cut(s) 395, 404, 1084, 1854
HpyF3I CTNAG 6 cut(s) 13, 44, 639, 1175, 1192, 1632
HpySE526I ACGT 1 cut(s) 368
HspAI GCGC 3 cut(s) 189, 396, 1465
Kpn2I TCCGGA 2 cut(s) 684, 1699
KroI GCCGGC 1 cut(s) 1613
KroNI GCCGGC 1 cut(s) 1615
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
LguI GCTCTTC 1 cut(s) 1291
LmnI GCTCC 4 cut(s) 106, 774, 843, 1382
Lsp1109I GCAGC 5 cut(s) 820, 822, 1225, 1239, 1262
LweI GCATC 5 cut(s) 244, 534, 624, 1116, 1329
MaeI CTAG 3 cut(s) 525, 899, 1079
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 72
MalI GATC 7 cut(s) 59, 693, 974, 1287, 1394, 1684, 1725
MbiI CCGCTC 1 cut(s) 867
MboI GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
MboII GAAGA 9 cut(s) 655, 1044, 1280, 1308, 1523, 1542, 1567, 1756, 1810
MfeI CAATTG 2 cut(s) 299, 1779
MflI RGATCY 3 cut(s) 57, 1392, 1682
MhlI GDGCHC 8 cut(s) 50, 111, 764, 898, 1189, 1257, 1352, 1375
MluCI AATT 9 cut(s) 299, 481, 621, 646, 947, 1060, 1604, 1779, 1834
MlyI GAGTC 3 cut(s) 338, 947, 1851
MmeI TCCRAC 1 cut(s) 391
Mph1103I ATGCAT 2 cut(s) 259, 1089
MroI TCCGGA 2 cut(s) 684, 1699
MroNI GCCGGC 1 cut(s) 1613
MroXI GAANNNNTTC 2 cut(s) 1818, 1886
MseI TTAA 3 cut(s) 1026, 1110, 1167
MslI CAYNNNNRTG 6 cut(s) 275, 435, 495, 497, 980, 1401
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 8 cut(s) 54, 251, 685, 1302, 1614, 1700, 1809, 1861
MspR9I CCNGG 9 cut(s) 54, 55, 551, 1004, 1303, 1566, 1809, 1810, 1862
MunI CAATTG 2 cut(s) 299, 1779
Mva1269I GAATGC 4 cut(s) 257, 667, 732, 1501
MvaI CCWGG 3 cut(s) 551, 1004, 1566
MvnI CGCG 2 cut(s) 63, 1896
MwoI GCNNNNNNNGC 4 cut(s) 395, 404, 1084, 1854
NaeI GCCGGC 1 cut(s) 1615
NciI CCSGG 6 cut(s) 54, 55, 1303, 1809, 1810, 1862
NcoI CCATGG 2 cut(s) 165, 1396
NdeII GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
NgoMIV GCCGGC 1 cut(s) 1613
NheI GCTAGC 1 cut(s) 1078
NlaIV GGNNCC 3 cut(s) 59, 770, 1563
NmeAIII GCCGAG 2 cut(s) 120, 330
NsiI ATGCAT 2 cut(s) 259, 1089
NspI RCATGY 3 cut(s) 496, 985, 1185
PagI TCATGA 1 cut(s) 132
PciI ACATGT 2 cut(s) 492, 981
PciSI GCTCTTC 1 cut(s) 1291
PctI GAATGC 4 cut(s) 257, 667, 732, 1501
PdiI GCCGGC 1 cut(s) 1615
PdmI GAANNNNTTC 2 cut(s) 1818, 1886
PfeI GAWTC 3 cut(s) 86, 487, 1747
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 826
PfoI TCCNGGA 1 cut(s) 1564
PkrI GCNGC 8 cut(s) 80, 151, 810, 837, 1105, 1215, 1229, 1277
PleI GAGTC 3 cut(s) 338, 947, 1850
PpsI GAGTC 3 cut(s) 338, 947, 1850
PscI ACATGT 2 cut(s) 492, 981
Psp124BI GAGCTC 2 cut(s) 50, 111
Psp6I CCWGG 3 cut(s) 549, 1002, 1564
PspGI CCWGG 3 cut(s) 549, 1002, 1564
PspN4I GGNNCC 3 cut(s) 59, 770, 1563
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 3 cut(s) 57, 1392, 1682
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 6 cut(s) 326, 365, 587, 754, 986, 1220
RsaNI GTAC 6 cut(s) 325, 364, 586, 753, 985, 1219
RseI CAYNNNNRTG 6 cut(s) 275, 435, 495, 497, 980, 1401
SacI GAGCTC 2 cut(s) 50, 111
SapI GCTCTTC 1 cut(s) 1291
SaqAI TTAA 3 cut(s) 1026, 1110, 1167
SatI GCNGC 8 cut(s) 79, 150, 809, 836, 1104, 1214, 1228, 1276
Sau3AI GATC 7 cut(s) 57, 691, 972, 1285, 1392, 1682, 1723
ScaI AGTACT 1 cut(s) 1220
SchI GAGTC 3 cut(s) 338, 947, 1851
ScrFI CCNGG 9 cut(s) 54, 55, 551, 1004, 1303, 1566, 1809, 1810, 1862
SduI GDGCHC 8 cut(s) 50, 111, 764, 898, 1189, 1257, 1352, 1375
SfaNI GCATC 5 cut(s) 244, 534, 624, 1116, 1329
SfcI CTRYAG 1 cut(s) 1686
SmaI CCCGGG 2 cut(s) 55, 1810
SmiMI CAYNNNNRTG 6 cut(s) 275, 435, 495, 497, 980, 1401
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 9 cut(s) 299, 481, 621, 646, 947, 1060, 1604, 1779, 1834
SspMI CTAG 3 cut(s) 525, 899, 1079
SstI GAGCTC 2 cut(s) 50, 111
StyD4I CCNGG 9 cut(s) 52, 53, 549, 1002, 1301, 1564, 1807, 1808, 1860
StyI CCWWGG 2 cut(s) 165, 1396
TaaI ACNGT 6 cut(s) 72, 274, 1247, 1766, 1819, 1949
TaiI ACGT 1 cut(s) 371
TaqI TCGA 8 cut(s) 95, 234, 627, 849, 956, 1119, 1659, 1880
TasI AATT 9 cut(s) 299, 481, 621, 646, 947, 1060, 1604, 1779, 1834
TatI WGTACW 3 cut(s) 324, 984, 1218
TauI GCSGC 3 cut(s) 81, 152, 1106
TfiI GAWTC 3 cut(s) 86, 487, 1747
Tru1I TTAA 3 cut(s) 1026, 1110, 1167
Tru9I TTAA 3 cut(s) 1026, 1110, 1167
TscAI CASTG 1 cut(s) 1771
TseI GCWGC 5 cut(s) 808, 835, 1213, 1227, 1275
TspDTI ATGAA 6 cut(s) 250, 592, 1044, 1553, 1617, 1677
TspGWI ACGGA 1 cut(s) 346
TspMI CCCGGG 2 cut(s) 53, 1808
TspRI CASTG 1 cut(s) 1771
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 826
VneI GTGCAC 1 cut(s) 760
XagI CCTNNNNNAGG 1 cut(s) 1007
XapI RAATTY 4 cut(s) 621, 646, 1604, 1834
XbaI TCTAGA 1 cut(s) 898
XceI RCATGY 3 cut(s) 496, 985, 1185
XcmI CCANNNNNNNNNTGG 1 cut(s) 1582
XmaI CCCGGG 2 cut(s) 53, 1808
XmnI GAANNNNTTC 2 cut(s) 1818, 1886
XspI CTAG 3 cut(s) 525, 899, 1079
ZrmI AGTACT 1 cut(s) 1220
Zsp2I ATGCAT 2 cut(s) 259, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.