MD16G1136500.v1.1

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013694
Physical Location & Seq
Forward (+)
10444938 .. 10446821
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1136500.v1.1.491

Sequence Viewer

Length: 1884 bp
ATGACTTCGCTGCTAGTCCTTCAACCCACCGGTCCCACCCCAGCCTCCCTAAGCTCGCCCAGGACCCACATTTCACCGTTACGCGGCCTGGAATCATGCTCTACCATGGCGGAGCTCAAGCAGTTGCACTCCCAAGTCATCAGGCTCGGCCTCGCCGCCGACAACGACGCTATGGGCCGGGTCATCAAGTTCTGCGCCCTCTCCAAAAATGGCGATTTGGGTTATGCCCTCCAGGTGTTCGACGCAATGCCTCAACCGGACGCATTCATTTACAACACCGTAATGAGAGGTTACTTGCAGTACCAATTGCCGAGAGATTGCATTGTTTTGTACTCGCAGATGTTGCAGGACTTTGTGACTCCAAATAGGTTCACGTTTCCGTCTGTTGTACGAGCTTGTTGCGCTGATGGTGCTGTTGGAGAAGGGAAACAAGTCCATGCCCATGTCGTTAAACTCGGGTTTGGAGATGATGGGTTTTGTCAGAACAATTTGATTCACATGTATGTGAAGTTTCAGTCTTTGGACGAAGCTAGAAGGGTGTTTGACAAGATGCCCCGGGTGGATGTTGTGTCTTGGACGACTTTGATTGCTGGGTACTCCCAGTGGGGCTTTGTCGATGAGGCTTTTGAAATGTTCGAGTTGATGCCCGAGAAGAATTCTGTATCTTGGAATGCAATGATTTCCTCTTATGTCCAAAGTGATCGTTTTCATGAGGCGTTTGCTTTGTTTCAAAGAATGCGTGTGGAAAACGTAGAATTGGATAAGTTCATGGCGGCTAGTATGTTATCAGCTTGTACTGGTTTAGGTGCTCTAGAACAAGGGAAGTGGATACATGGATATATTGAGAAAAGTGGGATTGAGTTGGACTCGAAACTTGCAACAACGATCATTGACATGTATTGCAAATGTGGCTGCCTGGAGAAGGCTTTTGAAGTTTTCAATGGTTTGCCCCGTAAAGGGATTTCTTCATGGAATTGCATGATTGGCGGGCTAGCAATGCACGGGAAAGGAGAGGCAGCCGTTGAGCTTTTCGAGCAGATGCAAAGGGACATGGTAGCTCCCGACAACATTACTTTTGTTAATGTTCTGAGTGCATGTGCTCACTCAGGGTTAGTAGAAAAGGGGCAGCAGTACTTCCGCTGCATGGTTGAAGTCCACGGTATTGAGCCCCGAACAGAGCATTTTGGCTGCATGGTTGATCTTCTTGGACGAGCCGGGAGGCTGGAGGAAGCAAGAAAGCTAATAAACGAGATGCCTATGAGCCCCGATGTAGGTGTCTTGGGTGCTCTCCTCGGAGCTTGTAAGATCCATGGAAATGTTGAACTGGGAGATGAAATAGGGAGGAGGGTAATCGAACTGGAACCAGAAAATAGCGGGCGCTATGTGTTGTTAGCCAATCTATACGCAAATGCGGGCAGATGGGACGATGTTGCCAATGTGAGGAGGTTGATGAATGACAGAGGAGTGAAGAAGGTTCCTGGATTTTCCATGATTGAATTGGAAGGCGCTGTCAGTGAATTCATTGCCGGCGGAGGGTCTCATCCTCAGACAAAAGAGATATACTCGAAAGTGGATGAAATGTTGAAATGTATAAGATCTGCAGGATATGTTCCGGACACAGAGGGAGTGTTGCATGACCTCGACGAGGAGGAAAAGGAGAACCCTTTATACTACCACAGTGAGAAACTTGCAATTGCATATGGCCTGTTGAAGACAAAACCCAGGGAAACTCTTCGTATATCGAAGAATTTACGAGTCTGCAAAGACTGCCACCAGGCAAGCAAGCTGATCTCAAAGGTTTTCGACCGTGAAATAATCGTAAGGGATAGAAATCGCTTCCACCATTTTAAAGGGGGAGAGTGTTCTTGTAAAGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

628

Amino Acids

70.18

Weight (kDa)

6.09

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 134 2.3e-08 PPR repeat family
PPR_1 PF12854 188 - 215 9.9e-06 PPR repeat
PPR PF01535 190 - 219 3.9e-08 PPR repeat
PPR_2 PF13041 218 - 265 2.8e-08 PPR repeat family
PPR PF01535 221 - 250 1e-07 PPR repeat
PPR_2 PF13041 321 - 368 7.1e-11 PPR repeat family
PPR PF01535 323 - 350 7.3e-07 PPR repeat
E_motif PF20431 437 - 499 2.6e-20 E motif
DYW_deaminase PF14432 535 - 627 4.4e-42 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 1612
AciI CCGC 9 cut(s) 84, 110, 156, 773, 987, 1138, 1374, 1412, 1530
AclWI GGATC 1 cut(s) 1300
AcsI RAATTY 3 cut(s) 655, 1517, 1747
AfaI GTAC 6 cut(s) 302, 332, 390, 596, 796, 1133
AfiI CCNNNNNNNGG 9 cut(s) 83, 922, 956, 957, 1144, 1271, 1440, 1533, 1645
AflIII ACRYGT 2 cut(s) 498, 894
AgeI ACCGGT 1 cut(s) 29
AjnI CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
Alw21I GWGCWC 4 cut(s) 117, 811, 1102, 1288
Alw26I GTCTC 1 cut(s) 1542
AlwI GGATC 1 cut(s) 1300
Ama87I CYCGRG 3 cut(s) 455, 555, 647
Aor13HI TCCGGA 1 cut(s) 1612
AoxI GGCC 4 cut(s) 85, 148, 175, 1702
ApeKI GCWGC 6 cut(s) 10, 912, 1016, 1126, 1140, 1188
ApoI RAATTY 3 cut(s) 655, 1517, 1747
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 1731
AspLEI GCGC 4 cut(s) 197, 404, 1380, 1508
AspS9I GGNCC 3 cut(s) 32, 63, 175
AsuC2I CCSGG 4 cut(s) 179, 556, 557, 1216
AsuHPI GGTGA 1 cut(s) 66
AsuNHI GCTAGC 1 cut(s) 991
AvaI CYCGRG 3 cut(s) 455, 555, 647
AvaII GGWCC 2 cut(s) 32, 63
BanII GRGCYC 3 cut(s) 117, 1170, 1265
BbsI GAAGAC 1 cut(s) 1718
Bbv12I GWGCWC 4 cut(s) 117, 811, 1102, 1288
BbvI GCAGC 5 cut(s) 899, 1028, 1127, 1138, 1175
BccI CCATC 3 cut(s) 401, 464, 1413
BceAI ACGGC 1 cut(s) 1004
BcgI CGANNNNNNTGC 2 cut(s) 381, 415
BciT130I CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 4 cut(s) 179, 556, 557, 1216
BcoDI GTCTC 1 cut(s) 1542
BfaI CTAG 5 cut(s) 14, 531, 777, 812, 992
BfmI CTRYAG 1 cut(s) 1599
BfoI RGCGCY 2 cut(s) 1381, 1509
BfuI GTATCC 1 cut(s) 822
BglII AGATCT 1 cut(s) 1595
BisI GCNGC 9 cut(s) 11, 85, 156, 774, 913, 1017, 1127, 1141, 1189
BlsI GCNGC 9 cut(s) 12, 86, 157, 775, 914, 1018, 1128, 1142, 1190
BmcAI AGTACT 1 cut(s) 1133
Bme18I GGWCC 2 cut(s) 32, 63
BmeT110I CYCGRG 3 cut(s) 455, 555, 647
BmgT120I GGNCC 3 cut(s) 32, 63, 175
BmiI GGNNCC 4 cut(s) 34, 65, 1362, 1476
BmrI ACTGGG 2 cut(s) 595, 1334
BmsI GCATC 4 cut(s) 540, 633, 1029, 1242
BmtI GCTAGC 1 cut(s) 995
BmuI ACTGGG 2 cut(s) 595, 1334
BpiI GAAGAC 1 cut(s) 1718
BplI GAGNNNNNCTC 4 cut(s) 851, 883, 1547, 1579
BpmI CTGGAG 3 cut(s) 215, 938, 1244
Bpu10I CCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 4 cut(s) 179, 556, 557, 1216
BsaBI GATNNNNATC 1 cut(s) 1830
BsaI GGTCTC 1 cut(s) 1542
BsaJI CCNNGG 9 cut(s) 59, 105, 554, 555, 1156, 1291, 1309, 1721, 1722
BsaWI WCCGGW 3 cut(s) 29, 256, 1612
Bsc4I CCNNNNNNNGG 9 cut(s) 83, 922, 956, 957, 1144, 1271, 1440, 1533, 1645
Bse118I RCCGGY 2 cut(s) 29, 1526
Bse1I ACTGG 5 cut(s) 601, 802, 1329, 1362, 1883
Bse3DI GCAATG 4 cut(s) 252, 681, 1002, 1521
Bse8I GATNNNNATC 1 cut(s) 1830
BseAI TCCGGA 1 cut(s) 1612
BseBI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
BseDI CCNNGG 9 cut(s) 59, 105, 554, 555, 1156, 1291, 1309, 1721, 1722
BseGI GGATG 3 cut(s) 568, 1540, 1579
BseJI GATNNNNATC 1 cut(s) 1830
BseLI CCNNNNNNNGG 9 cut(s) 83, 922, 956, 957, 1144, 1271, 1440, 1533, 1645
BseMI GCAATG 4 cut(s) 252, 681, 1002, 1521
BseMII CTCAG 3 cut(s) 1079, 1119, 1559
BseNI ACTGG 5 cut(s) 601, 802, 1329, 1362, 1883
BseRI GAGGAG 5 cut(s) 1280, 1357, 1456, 1476, 1661
BseXI GCAGC 5 cut(s) 899, 1028, 1127, 1138, 1175
BseYI CCCAGC 2 cut(s) 40, 590
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 1807
BshFI GGCC 4 cut(s) 87, 150, 177, 1704
BshTI ACCGGT 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 1807
BsiHKAI GWGCWC 4 cut(s) 117, 811, 1102, 1288
BsiHKCI CYCGRG 3 cut(s) 455, 555, 647
BsiSI CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
BslFI GGGAC 3 cut(s) 18, 1061, 1436
BslI CCNNNNNNNGG 9 cut(s) 83, 922, 956, 957, 1144, 1271, 1440, 1533, 1645
BsmAI GTCTC 1 cut(s) 1542
BsmFI GGGAC 3 cut(s) 18, 1061, 1436
BsmI GAATGC 3 cut(s) 263, 676, 741
BsnI GGCC 4 cut(s) 87, 150, 177, 1704
Bso31I GGTCTC 1 cut(s) 1542
BsoBI CYCGRG 3 cut(s) 455, 555, 647
Bsp1286I GDGCHC 6 cut(s) 117, 811, 1102, 1170, 1265, 1288
Bsp13I TCCGGA 1 cut(s) 1612
Bsp143I GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
Bsp19I CCATGG 2 cut(s) 105, 1309
BspACI CCGC 9 cut(s) 84, 110, 156, 773, 987, 1138, 1374, 1412, 1530
BspANI GGCC 4 cut(s) 87, 150, 177, 1704
BspCNI CTCAG 3 cut(s) 1080, 1118, 1558
BspEI TCCGGA 1 cut(s) 1612
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 4 cut(s) 34, 65, 1362, 1476
BspMAI CTGCAG 1 cut(s) 1603
BspOI GCTAGC 1 cut(s) 995
BspPI GGATC 1 cut(s) 1300
BspTNI GGTCTC 1 cut(s) 1542
BsrDI GCAATG 4 cut(s) 252, 681, 1002, 1521
BsrFI RCCGGY 2 cut(s) 29, 1526
BsrI ACTGG 5 cut(s) 601, 802, 1329, 1362, 1883
BssAI RCCGGY 2 cut(s) 29, 1526
BssECI CCNNGG 9 cut(s) 59, 105, 554, 555, 1156, 1291, 1309, 1721, 1722
BssMI GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
BssT1I CCWWGG 2 cut(s) 105, 1309
Bst2UI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
Bst4CI ACNGT 5 cut(s) 78, 280, 1160, 1679, 1808
Bst6I CTCTTC 1 cut(s) 1737
BstAPI GCANNNNNTGC 2 cut(s) 343, 1767
BstC8I GCNNGC 8 cut(s) 56, 989, 993, 1376, 1414, 1528, 1780, 1784
BstDEI CTNAG 4 cut(s) 50, 1088, 1105, 1545
BstDSI CCRYGG 3 cut(s) 105, 1156, 1309
BstENI CCTNNNNNAGG 2 cut(s) 920, 1643
BstF5I GGATG 3 cut(s) 568, 1540, 1579
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 2 cut(s) 1381, 1509
BstHHI GCGC 4 cut(s) 197, 404, 1380, 1508
BstKTI GATC 6 cut(s) 703, 888, 1201, 1308, 1598, 1791
BstMAI GTCTC 1 cut(s) 1542
BstMBI GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
BstMCI CGRYCG 1 cut(s) 1807
BstMWI GCNNNNNNNGC 8 cut(s) 343, 401, 410, 909, 984, 997, 1033, 1767
BstNI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
BstNSI RCATGY 3 cut(s) 502, 898, 1098
BstSFI CTRYAG 1 cut(s) 1599
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 899, 1028, 1127, 1138, 1175
BstV2I GAAGAC 1 cut(s) 1718
BstX2I RGATCY 2 cut(s) 1305, 1595
BstYI RGATCY 2 cut(s) 1305, 1595
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 4 cut(s) 87, 150, 177, 1704
BtgI CCRYGG 3 cut(s) 105, 1156, 1309
BtsCI GGATG 3 cut(s) 568, 1540, 1579
BtsIMutI CAGTG 3 cut(s) 608, 1519, 1684
Cac8I GCNNGC 8 cut(s) 56, 989, 993, 1376, 1414, 1528, 1780, 1784
CciI TCATGA 1 cut(s) 709
CfoI GCGC 4 cut(s) 197, 404, 1380, 1508
Cfr10I RCCGGY 2 cut(s) 29, 1526
Cfr13I GGNCC 3 cut(s) 32, 63, 175
Cfr9I CCCGGG 1 cut(s) 555
CseI GACGC 3 cut(s) 176, 251, 269
Csp6I GTAC 6 cut(s) 301, 331, 389, 595, 795, 1132
CspAI ACCGGT 1 cut(s) 29
CspCI CAANNNNNGTGG 2 cut(s) 806, 841
CviQI GTAC 6 cut(s) 301, 331, 389, 595, 795, 1132
DdeI CTNAG 4 cut(s) 50, 1088, 1105, 1545
DpnI GATC 6 cut(s) 702, 887, 1200, 1307, 1597, 1790
DpnII GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
DraI TTTAAA 1 cut(s) 1849
Eam1104I CTCTTC 1 cut(s) 1737
EarI CTCTTC 1 cut(s) 1737
EciI GGCGGA 2 cut(s) 125, 1545
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 105, 1309
Eco24I GRGCYC 3 cut(s) 117, 1170, 1265
Eco31I GGTCTC 1 cut(s) 1542
Eco47I GGWCC 2 cut(s) 32, 63
Eco53kI GAGCTC 1 cut(s) 115
Eco88I CYCGRG 3 cut(s) 455, 555, 647
EcoICRI GAGCTC 1 cut(s) 115
EcoNI CCTNNNNNAGG 2 cut(s) 920, 1643
EcoO109I RGGNCCY 1 cut(s) 63
EcoRI GAATTC 2 cut(s) 655, 1517
EcoRII CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
EcoT14I CCWWGG 2 cut(s) 105, 1309
EcoT38I GRGCYC 3 cut(s) 117, 1170, 1265
ErhI CCWWGG 2 cut(s) 105, 1309
FaqI GGGAC 3 cut(s) 18, 1061, 1436
FauI CCCGC 3 cut(s) 980, 1367, 1405
FauNDI CATATG 1 cut(s) 1699
Fnu4HI GCNGC 9 cut(s) 11, 85, 156, 774, 913, 1017, 1127, 1141, 1189
FokI GGATG 3 cut(s) 575, 1527, 1586
FriOI GRGCYC 3 cut(s) 117, 1170, 1265
Fsp4HI GCNGC 9 cut(s) 11, 85, 156, 774, 913, 1017, 1127, 1141, 1189
FspBI CTAG 5 cut(s) 14, 531, 777, 812, 992
GlaI GCGC 4 cut(s) 196, 403, 1379, 1507
GluI GCNGC 9 cut(s) 11, 85, 156, 774, 913, 1017, 1127, 1141, 1189
GsaI CCCAGC 2 cut(s) 44, 594
GsuI CTGGAG 3 cut(s) 215, 938, 1244
HaeII RGCGCY 2 cut(s) 1381, 1509
HaeIII GGCC 4 cut(s) 87, 150, 177, 1704
HapII CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
HgaI GACGC 3 cut(s) 176, 251, 269
HhaI GCGC 4 cut(s) 197, 404, 1380, 1508
Hin6I GCGC 4 cut(s) 195, 402, 1378, 1506
HinP1I GCGC 4 cut(s) 195, 402, 1378, 1506
HinfI GANTC 5 cut(s) 92, 358, 493, 866, 1755
HpaII CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 2 cut(s) 372, 1156
Hpy188I TCNGA 4 cut(s) 483, 1089, 1295, 1548
Hpy188III TCNNGA 4 cut(s) 710, 812, 1061, 1613
Hpy8I GTNNAC 2 cut(s) 372, 1156
Hpy99I CGWCG 3 cut(s) 170, 245, 1646
HpyAV CCTTC 6 cut(s) 29, 416, 528, 916, 1465, 1496
HpyCH4III ACNGT 5 cut(s) 78, 280, 1160, 1679, 1808
HpyCH4IV ACGT 2 cut(s) 374, 750
HpyF10VI GCNNNNNNNGC 8 cut(s) 343, 401, 410, 909, 984, 997, 1033, 1767
HpyF3I CTNAG 4 cut(s) 50, 1088, 1105, 1545
HpySE526I ACGT 2 cut(s) 374, 750
HspAI GCGC 4 cut(s) 195, 402, 1378, 1506
Kpn2I TCCGGA 1 cut(s) 1612
KroI GCCGGC 1 cut(s) 1526
KroNI GCCGGC 1 cut(s) 1528
Kzo9I GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
LmnI GCTCC 3 cut(s) 112, 1063, 1295
Lsp1109I GCAGC 5 cut(s) 899, 1028, 1127, 1138, 1175
LweI GCATC 4 cut(s) 540, 633, 1029, 1242
MaeI CTAG 5 cut(s) 14, 531, 777, 812, 992
MaeII ACGT 2 cut(s) 374, 750
MaeIII GTNAC 3 cut(s) 78, 290, 355
MalI GATC 6 cut(s) 702, 887, 1200, 1307, 1597, 1790
MboI GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
MboII GAAGA 7 cut(s) 664, 957, 1193, 1480, 1723, 1724, 1756
MfeI CAATTG 2 cut(s) 305, 1692
MflI RGATCY 2 cut(s) 1305, 1595
MhlI GDGCHC 6 cut(s) 117, 811, 1102, 1170, 1265, 1288
MluCI AATT 9 cut(s) 305, 487, 655, 755, 973, 1496, 1517, 1692, 1747
MlyI GAGTC 3 cut(s) 352, 860, 1764
MmeI TCCRAC 2 cut(s) 397, 843
MroI TCCGGA 1 cut(s) 1612
MroNI GCCGGC 1 cut(s) 1526
MroXI GAANNNNTTC 1 cut(s) 1731
MseI TTAA 3 cut(s) 450, 1080, 1848
MslI CAYNNNNRTG 6 cut(s) 281, 441, 501, 503, 893, 1314
MspA1I CMGCKG 1 cut(s) 1140
MspI CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
MunI CAATTG 2 cut(s) 305, 1692
Mva1269I GAATGC 3 cut(s) 263, 676, 741
MvaI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 8 cut(s) 343, 401, 410, 909, 984, 997, 1033, 1767
NaeI GCCGGC 1 cut(s) 1528
NciI CCSGG 4 cut(s) 179, 556, 557, 1216
NcoI CCATGG 2 cut(s) 105, 1309
NdeI CATATG 1 cut(s) 1699
NdeII GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
NgoMIV GCCGGC 1 cut(s) 1526
NheI GCTAGC 1 cut(s) 991
NlaIV GGNNCC 4 cut(s) 34, 65, 1362, 1476
NmeAIII GCCGAG 2 cut(s) 126, 336
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 3 cut(s) 502, 898, 1098
PagI TCATGA 1 cut(s) 709
PasI CCCWGGG 1 cut(s) 1722
PciI ACATGT 2 cut(s) 498, 894
PctI GAATGC 3 cut(s) 263, 676, 741
PdiI GCCGGC 1 cut(s) 1528
PdmI GAANNNNTTC 1 cut(s) 1731
PfeI GAWTC 2 cut(s) 92, 493
PfoI TCCNGGA 1 cut(s) 1477
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 9 cut(s) 12, 86, 157, 775, 914, 1018, 1128, 1142, 1190
PleI GAGTC 3 cut(s) 352, 860, 1763
PpsI GAGTC 3 cut(s) 352, 860, 1763
PpuMI RGGWCCY 1 cut(s) 63
PscI ACATGT 2 cut(s) 498, 894
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
PspFI CCCAGC 2 cut(s) 40, 590
PspGI CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
PspN4I GGNNCC 4 cut(s) 34, 65, 1362, 1476
PspPI GGNCC 3 cut(s) 32, 63, 175
PspPPI RGGWCCY 1 cut(s) 63
PsrI GAACNNNNNNTAC 2 cut(s) 1652, 1684
PstI CTGCAG 1 cut(s) 1603
PsuI RGATCY 2 cut(s) 1305, 1595
RsaI GTAC 6 cut(s) 302, 332, 390, 596, 796, 1133
RsaNI GTAC 6 cut(s) 301, 331, 389, 595, 795, 1132
RseI CAYNNNNRTG 6 cut(s) 281, 441, 501, 503, 893, 1314
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 450, 1080, 1848
SatI GCNGC 9 cut(s) 11, 85, 156, 774, 913, 1017, 1127, 1141, 1189
Sau3AI GATC 6 cut(s) 700, 885, 1198, 1305, 1595, 1788
Sau96I GGNCC 3 cut(s) 32, 63, 175
ScaI AGTACT 1 cut(s) 1133
SchI GAGTC 3 cut(s) 352, 860, 1764
SduI GDGCHC 6 cut(s) 117, 811, 1102, 1170, 1265, 1288
SfaNI GCATC 4 cut(s) 540, 633, 1029, 1242
SfcI CTRYAG 1 cut(s) 1599
SinI GGWCC 2 cut(s) 32, 63
SmaI CCCGGG 1 cut(s) 557
SmiMI CAYNNNNRTG 6 cut(s) 281, 441, 501, 503, 893, 1314
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 9 cut(s) 305, 487, 655, 755, 973, 1496, 1517, 1692, 1747
SsiI CCGC 9 cut(s) 84, 110, 156, 773, 987, 1138, 1374, 1412, 1530
SspMI CTAG 5 cut(s) 14, 531, 777, 812, 992
SstI GAGCTC 1 cut(s) 117
StyI CCWWGG 2 cut(s) 105, 1309
TaaI ACNGT 5 cut(s) 78, 280, 1160, 1679, 1808
TaiI ACGT 2 cut(s) 377, 753
TasI AATT 9 cut(s) 305, 487, 655, 755, 973, 1496, 1517, 1692, 1747
TatI WGTACW 3 cut(s) 330, 794, 1131
TauI GCSGC 3 cut(s) 87, 158, 776
TfiI GAWTC 2 cut(s) 92, 493
Tru1I TTAA 3 cut(s) 450, 1080, 1848
Tru9I TTAA 3 cut(s) 450, 1080, 1848
TscAI CASTG 3 cut(s) 608, 1519, 1684
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 6 cut(s) 10, 912, 1016, 1126, 1140, 1188
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 8 cut(s) 256, 698, 757, 957, 1347, 1466, 1510, 1590
TspGWI ACGGA 1 cut(s) 369
TspMI CCCGGG 1 cut(s) 555
TspRI CASTG 3 cut(s) 608, 1519, 1684
VpaK11BI GGWCC 2 cut(s) 32, 63
XagI CCTNNNNNAGG 2 cut(s) 920, 1643
XapI RAATTY 3 cut(s) 655, 1517, 1747
XbaI TCTAGA 1 cut(s) 811
XceI RCATGY 3 cut(s) 502, 898, 1098
XcmI CCANNNNNNNNNTGG 1 cut(s) 1495
XmaI CCCGGG 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 1731
XspI CTAG 5 cut(s) 14, 531, 777, 812, 992
ZrmI AGTACT 1 cut(s) 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.