MD13G1162300.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014068
Physical Location & Seq
Reverse (-)
12772092 .. 12774009
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1162300.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGGGCAGGATTCTGATTTGAAATCCCAGCTGGTGACTGAGATTTGCTCCGTTTCCGCGCGCGCCATTGCCTGCTCTCACCGGCATGGCGGCGGCAGCAGCCCTGTTCATTCGTCTTTCGTCGATTGGTATCGCCTCCTTCGAGTGGAGGAAAATGCTGGCGCGGATGTTATAAGAAAGAGATATCATGAACTTGCTTTACAACTTCATCCAGATAAGAACAAACACCCAAAAGCTGAAATTGCATTCAAGCTTGTTTCTGAGGCATATTCATGTCTCTCAGATGATGCAAAAAGAAGAGTTTTTGACTTGGAGAGATGGAGGAGGTTCTGCTCTGAGTGTGGCACAATCACCTACACAACAACCCACCACAACTCCCCAAGCACTGCCAATCCCAATCCTTCTTCACATCACAAGCCTTACAATCCCACAAATTCAAGGCCTTGCAAAGTAACCAAGGGACTGAAAGATGTCAGAAACAGATTCAGAGAGGAGGTAAGGGTGATAGAAAGCTGCTTGAAGGCCAATGCAGCTGCAGGAACACCAGGAAAGCAATCCTCCTCCTCACTATTTACTCCACCTGCTGCTTTTGATGCGTTTCAGAGCAGGTTCCCAAGGGAATCCCCTGTCTTCAACCCATCTGACCATAAGGTTCAGGGATACCCTCATCTTCGGGCTCGAATTTACGAGAAGCCGAAGAACTTTTGGCAGTTGAGAACTGGGCATCATGTACTCAATTATGAGCAGGGTAGAGGTAGTTATGATTCTCCAGTGTTTGAGGTCAGATCGGATAGTGGGATGTTTAAGAGTAGGTCTACTTGTGTTCGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

278

Amino Acids

31.35

Weight (kDa)

9.54

Isoelectric Point (pI)

65.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 42 - 103 1.1e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06778
fragaria_vesca FvH4_4g18890
malus_domestica MD13G1162300.v1.1 MD16G1162100.v1.1
prunus_persica Prupe.1G129600_v2.0.a1
pyrus_communis pycom13g13850 pycom16g13750
rosa_chinensis RchiOBHm_Chr4g0423791
rosa_multiflora Rmu_co8479069.1_g000001
rosa_roxburghii Rroxscaffold_5G00365900
rosa_rugosa Rorug04G0190600 Rorug04G0190700
rosa_samantha Rh4AG250100 Rh4BG255300 Rh4CG263300 Rh4DG249600
rosa_wichuraiana Rw4G021410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 173
AarI CACCTGC 1 cut(s) 589
Acc36I ACCTGC 2 cut(s) 589, 597
AccI GTMKAC 1 cut(s) 815
AccII CGCG 4 cut(s) 59, 61, 63, 164
AciI CCGC 4 cut(s) 57, 90, 93, 164
AcsI RAATTY 2 cut(s) 433, 681
AfaI GTAC 1 cut(s) 732
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 5 cut(s) 22, 250, 438, 520, 634
AjnI CCWGG 1 cut(s) 544
AluBI AGCT 5 cut(s) 31, 236, 253, 513, 533
AluI AGCT 5 cut(s) 31, 236, 253, 513, 533
Alw26I GTCTC 1 cut(s) 281
AoxI GGCC 2 cut(s) 440, 522
ApeKI GCWGC 6 cut(s) 96, 99, 513, 530, 533, 584
ApoI RAATTY 2 cut(s) 433, 681
AspLEI GCGC 4 cut(s) 61, 63, 65, 164
AsuHPI GGTGA 4 cut(s) 46, 71, 343, 514
BanII GRGCYC 1 cut(s) 679
BbsI GAAGAC 1 cut(s) 622
BbvI GCAGC 6 cut(s) 108, 111, 500, 520, 542, 571
BccI CCATC 2 cut(s) 312, 646
BciT130I CCWGG 1 cut(s) 546
BciVI GTATCC 1 cut(s) 653
BcoDI GTCTC 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 534
BfuAI ACCTGC 2 cut(s) 589, 597
BfuI GTATCC 1 cut(s) 653
BisI GCNGC 8 cut(s) 91, 94, 97, 100, 514, 531, 534, 585
BlsI GCNGC 8 cut(s) 92, 95, 98, 101, 515, 532, 535, 586
Bme1390I CCNGG 1 cut(s) 546
BmiI GGNNCC 1 cut(s) 611
BmrFI CCNGG 1 cut(s) 546
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 3 cut(s) 277, 583, 733
BmuI ACTGGG 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 622
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 456, 614
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 2 cut(s) 724, 770
Bse3DI GCAATG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 2 cut(s) 456, 614
BseGI GGATG 3 cut(s) 172, 208, 804
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 4 cut(s) 30, 252, 294, 327
BseNI ACTGG 2 cut(s) 724, 770
BsePI GCGCGC 2 cut(s) 59, 61
BseRI GAGGAG 4 cut(s) 337, 506, 550, 553
BseXI GCAGC 6 cut(s) 108, 111, 500, 520, 542, 571
BseYI CCCAGC 1 cut(s) 27
Bsh1236I CGCG 4 cut(s) 59, 61, 63, 164
BshFI GGCC 2 cut(s) 442, 524
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 474
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 2 cut(s) 442, 524
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 1 cut(s) 785
BspACI CCGC 4 cut(s) 57, 90, 93, 164
BspANI GGCC 2 cut(s) 442, 524
BspCNI CTCAG 4 cut(s) 31, 253, 293, 328
BspFNI CGCG 4 cut(s) 59, 61, 63, 164
BspHI TCATGA 2 cut(s) 187, 830
BspLI GGNNCC 1 cut(s) 611
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 2 cut(s) 589, 597
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 81
BsrI ACTGG 2 cut(s) 724, 770
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 456, 614
BssHII GCGCGC 2 cut(s) 59, 61
BssMI GATC 1 cut(s) 785
BssT1I CCWWGG 2 cut(s) 456, 614
Bst2UI CCWGG 1 cut(s) 546
Bst6I CTCTTC 1 cut(s) 292
BstC8I GCNNGC 4 cut(s) 61, 63, 73, 160
BstDEI CTNAG 4 cut(s) 39, 261, 280, 336
BstF5I GGATG 3 cut(s) 172, 208, 804
BstFNI CGCG 4 cut(s) 59, 61, 63, 164
BstHHI GCGC 4 cut(s) 61, 63, 65, 164
BstKTI GATC 1 cut(s) 788
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 1 cut(s) 785
BstMWI GCNNNNNNNGC 5 cut(s) 96, 99, 242, 530, 593
BstNI CCWGG 1 cut(s) 546
BstSCI CCNGG 1 cut(s) 544
BstSFI CTRYAG 1 cut(s) 534
BstUI CGCG 4 cut(s) 59, 61, 63, 164
BstV1I GCAGC 6 cut(s) 108, 111, 500, 520, 542, 571
BstV2I GAAGAC 1 cut(s) 622
BsuI GTATCC 1 cut(s) 653
BsuRI GGCC 2 cut(s) 442, 524
BtsCI GGATG 3 cut(s) 172, 208, 804
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 2 cut(s) 384, 777
BveI ACCTGC 2 cut(s) 589, 597
Cac8I GCNNGC 4 cut(s) 61, 63, 73, 160
CciI TCATGA 2 cut(s) 187, 830
CfoI GCGC 4 cut(s) 61, 63, 65, 164
Cfr10I RCCGGY 1 cut(s) 81
Csp6I GTAC 1 cut(s) 731
CviAII CATG 5 cut(s) 86, 188, 273, 728, 831
CviQI GTAC 1 cut(s) 731
DdeI CTNAG 4 cut(s) 39, 261, 280, 336
DpnI GATC 1 cut(s) 787
DpnII GATC 1 cut(s) 785
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 456, 614
Eco147I AGGCCT 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 679
Eco32I GATATC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 1 cut(s) 185
EcoT14I CCWWGG 2 cut(s) 456, 614
EcoT38I GRGCYC 1 cut(s) 679
ErhI CCWWGG 2 cut(s) 456, 614
FaeI CATG 5 cut(s) 89, 191, 276, 731, 834
FaqI GGGAC 1 cut(s) 474
FatI CATG 5 cut(s) 85, 187, 272, 727, 830
FblI GTMKAC 1 cut(s) 815
Fnu4HI GCNGC 8 cut(s) 91, 94, 97, 100, 514, 531, 534, 585
FokI GGATG 3 cut(s) 179, 195, 811
FriOI GRGCYC 1 cut(s) 679
Fsp4HI GCNGC 8 cut(s) 91, 94, 97, 100, 514, 531, 534, 585
GlaI GCGC 4 cut(s) 60, 62, 64, 163
GluI GCNGC 8 cut(s) 91, 94, 97, 100, 514, 531, 534, 585
GsaI CCCAGC 1 cut(s) 31
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 2 cut(s) 442, 524
HapII CCGG 1 cut(s) 82
HhaI GCGC 4 cut(s) 61, 63, 65, 164
Hin1II CATG 5 cut(s) 89, 191, 276, 731, 834
Hin6I GCGC 4 cut(s) 59, 61, 63, 162
HinP1I GCGC 4 cut(s) 59, 61, 63, 162
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 4 cut(s) 11, 483, 620, 764
HpaII CCGG 1 cut(s) 82
HphI GGTGA 4 cut(s) 46, 71, 343, 514
Hpy166II GTNNAC 1 cut(s) 816
Hpy188III TCNNGA 3 cut(s) 188, 212, 831
Hpy8I GTNNAC 1 cut(s) 816
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 149, 411, 514
HpyCH4V TGCA 5 cut(s) 245, 290, 447, 530, 536
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 99, 242, 530, 593
HpyF3I CTNAG 4 cut(s) 39, 261, 280, 336
Hsp92II CATG 5 cut(s) 89, 191, 276, 731, 834
HspAI GCGC 4 cut(s) 59, 61, 63, 162
Kzo9I GATC 1 cut(s) 785
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 6 cut(s) 108, 111, 500, 520, 542, 571
LweI GCATC 3 cut(s) 277, 583, 733
MaeIII GTNAC 2 cut(s) 34, 451
MalI GATC 1 cut(s) 787
MauBI CGCGCGCG 1 cut(s) 59
MboI GATC 1 cut(s) 785
MboII GAAGA 5 cut(s) 309, 396, 622, 662, 709
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 4 cut(s) 240, 433, 681, 736
MseI TTAA 1 cut(s) 804
MslI CAYNNNNRTG 2 cut(s) 84, 271
MspA1I CMGCKG 2 cut(s) 31, 533
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 4 cut(s) 59, 61, 63, 164
MwoI GCNNNNNNNGC 5 cut(s) 96, 99, 242, 530, 593
NdeII GATC 1 cut(s) 785
NlaIII CATG 5 cut(s) 89, 191, 276, 731, 834
NlaIV GGNNCC 1 cut(s) 611
NmuCI GTSAC 1 cut(s) 34
PagI TCATGA 2 cut(s) 187, 830
PaqCI CACCTGC 1 cut(s) 589
PauI GCGCGC 2 cut(s) 59, 61
PceI AGGCCT 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PctI GAATGC 1 cut(s) 245
PfeI GAWTC 4 cut(s) 11, 483, 620, 764
PkrI GCNGC 8 cut(s) 92, 95, 98, 101, 515, 532, 535, 586
PsiI TTATAA 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 611
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PstI CTGCAG 1 cut(s) 538
PteI GCGCGC 2 cut(s) 59, 61
PvuII CAGCTG 2 cut(s) 31, 533
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
RseI CAYNNNNRTG 2 cut(s) 84, 271
SaqAI TTAA 1 cut(s) 804
SatI GCNGC 8 cut(s) 91, 94, 97, 100, 514, 531, 534, 585
Sau3AI GATC 1 cut(s) 785
ScrFI CCNGG 1 cut(s) 546
SduI GDGCHC 1 cut(s) 679
SfaNI GCATC 3 cut(s) 277, 583, 733
SfcI CTRYAG 1 cut(s) 534
SmiMI CAYNNNNRTG 2 cut(s) 84, 271
Sse9I AATT 4 cut(s) 240, 433, 681, 736
SseBI AGGCCT 1 cut(s) 442
SsiI CCGC 4 cut(s) 57, 90, 93, 164
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 544
StyI CCWWGG 2 cut(s) 456, 614
TaqI TCGA 3 cut(s) 123, 142, 679
TasI AATT 4 cut(s) 240, 433, 681, 736
TatI WGTACW 1 cut(s) 730
TauI GCSGC 2 cut(s) 93, 96
TfiI GAWTC 4 cut(s) 11, 483, 620, 764
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TscAI CASTG 2 cut(s) 391, 777
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 6 cut(s) 96, 99, 513, 530, 533, 584
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 5 cut(s) 98, 197, 204, 261, 819
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 2 cut(s) 391, 777
XapI RAATTY 2 cut(s) 433, 681
XmiI GTMKAC 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.