MD16G1162100.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014068
Physical Location & Seq
Reverse (-)
13225389 .. 13227499
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1162100.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGTCTGGCTACAACTCCTCAAATTCCTCCACGTGGCAAGATTTAGGGACGACCCCTTCTTATTCCCCCGTTTTCCCACCAAGTCCAAGGGAGCTCTTTTCTAAACCGCTCCACCTCTCTCTCTCTCTCTCTCTCGCCAAACCAAATCCAACAATTCAATTTTCAAACATTTTTTCTCATTCTTTTCCATTTTCTCTGTTGGAGAGAAAAATGGGGCAGGATTCCGATTTGAAATCCCAGCTGGTGACTGAGATTTGCTCCATTTCCACGCGTGCCATTGCCTGCTCCCACAGGCATGGCAGCGGCAGCAGCAGCCCGGTTCGTTCGTCGTCTTTCGTCGATTGGTATCGCCTTCTTAGAGTGGAGGAAAATGCTGGGGCGGATGTTATAAGGAAGAGATATCATGAACTTGCTTTACAACTTCATCCAGATAAGAACAAGCACCCAAAAGCTGAAATTGCATTCAAGCTTGTTTCTGAGGCATATTCATGTCTCTCAGATACTGCAAAAAGAAGAGTTTTTGACTTGGAGAGATGGAGGAAGTTCTGCTTTGATTGTGGCACAATCCCCTACACGACAACCCACCACAACTCTCCCAGCACTGCCAATCCCAGTCCTTCTTCACACCACAAGCATTACAATCCCACTAATCCAAGGCCTTGCAAAGTAATCAAGGGACTGAAAGATATCAGCAGCAGATTCAGAGAGGAGGCAAGAGTGATAGAAAACTGCTTGAAGGCCAATGCAGCTGCAGGAAAACAGTCCTCCTCACTGTTTAGTCCACCTGCTGCTGCTTTTGATGTGTTTCGTAGCAGGTTCCCAAAAGAATCACCTGTATTCAATCCATCTGAACATAAGGTTCAGGGATACCCTCATCTTCGGGCTCGAATATACGAAAAACCGATCAAGTTTTGGCAGCTGAGAGCAGGGCATCATGTAGTGAATTATGAGCAGGGGAGAGCTGGTTATGATTCTCCAGTGTTTGAGGTCAGATCAGATAGAGGAATGTTTAAGAGTAGGTCTACTTGTGTTCGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

347

Amino Acids

39.12

Weight (kDa)

9.71

Isoelectric Point (pI)

64.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 114 - 175 2.5e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06778
fragaria_vesca FvH4_4g18890
malus_domestica MD13G1162300.v1.1 MD16G1162100.v1.1
prunus_persica Prupe.1G129600_v2.0.a1
pyrus_communis pycom13g13850 pycom16g13750
rosa_chinensis RchiOBHm_Chr4g0423791
rosa_multiflora Rmu_co8479069.1_g000001
rosa_roxburghii Rroxscaffold_5G00365900
rosa_rugosa Rorug04G0190600 Rorug04G0190700
rosa_samantha Rh4AG250100 Rh4BG255300 Rh4CG263300 Rh4DG249600
rosa_wichuraiana Rw4G021410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AarI CACCTGC 1 cut(s) 793
Acc36I ACCTGC 2 cut(s) 793, 804
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 1022
AccII CGCG 1 cut(s) 271
AciI CCGC 3 cut(s) 107, 303, 380
AcsI RAATTY 1 cut(s) 22
AcvI CACGTG 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 33
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 6 cut(s) 158, 165, 232, 466, 736, 841
AluBI AGCT 7 cut(s) 94, 241, 452, 469, 749, 919, 962
AluI AGCT 7 cut(s) 94, 241, 452, 469, 749, 919, 962
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 497
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 2 cut(s) 656, 738
ApoI RAATTY 1 cut(s) 22
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 256, 822
BanII GRGCYC 2 cut(s) 96, 886
BbrPI CACGTG 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 2 cut(s) 528, 853
BciVI GTATCC 1 cut(s) 860
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 750
BfuAI ACCTGC 2 cut(s) 793, 804
BfuI GTATCC 1 cut(s) 860
Bme1390I CCNGG 1 cut(s) 317
BmiI GGNNCC 1 cut(s) 818
BmrFI CCNGG 1 cut(s) 317
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 940
BmuI ACTGGG 1 cut(s) 606
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 1 cut(s) 960
BpuMI CCSGG 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 86, 653
BsaXI ACNNNNNCTCC 2 cut(s) 949, 979
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 612, 977
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 345
BseDI CCNNGG 2 cut(s) 86, 653
BseGI GGATG 2 cut(s) 388, 424
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 4 cut(s) 240, 468, 510, 911
BseNI ACTGG 2 cut(s) 612, 977
BseRI GAGGAG 3 cut(s) 7, 722, 757
BseYI CCCAGC 3 cut(s) 237, 374, 596
Bsh1236I CGCG 1 cut(s) 271
BshFI GGCC 2 cut(s) 658, 740
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 2 cut(s) 61, 690
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 497
BsmFI GGGAC 2 cut(s) 61, 690
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 658, 740
Bsp1286I GDGCHC 2 cut(s) 96, 886
Bsp143I GATC 2 cut(s) 903, 992
BspACI CCGC 3 cut(s) 107, 303, 380
BspANI GGCC 2 cut(s) 658, 740
BspCNI CTCAG 4 cut(s) 241, 469, 509, 912
BspFNI CGCG 1 cut(s) 271
BspHI TCATGA 2 cut(s) 403, 1037
BspLI GGNNCC 1 cut(s) 818
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 2 cut(s) 793, 804
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 2 cut(s) 612, 977
BssECI CCNNGG 2 cut(s) 86, 653
BssMI GATC 2 cut(s) 903, 992
BssT1I CCWWGG 2 cut(s) 86, 653
Bst4CI ACNGT 2 cut(s) 762, 774
Bst6I CTCTTC 2 cut(s) 389, 508
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 273, 283
BstDEI CTNAG 5 cut(s) 249, 356, 477, 496, 920
BstF5I GGATG 2 cut(s) 388, 424
BstFNI CGCG 1 cut(s) 271
BstKTI GATC 2 cut(s) 906, 995
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 2 cut(s) 903, 992
BstMWI GCNNNNNNNGC 5 cut(s) 306, 309, 312, 458, 746
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 296
BsuI GTATCC 1 cut(s) 860
BsuRI GGCC 2 cut(s) 658, 740
BtsCI GGATG 2 cut(s) 388, 424
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 3 cut(s) 600, 770, 984
BveI ACCTGC 2 cut(s) 793, 804
Cac8I GCNNGC 2 cut(s) 273, 283
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 2 cut(s) 403, 1037
CviAII CATG 5 cut(s) 296, 404, 489, 935, 1038
DdeI CTNAG 5 cut(s) 249, 356, 477, 496, 920
DpnI GATC 2 cut(s) 905, 994
DpnII GATC 2 cut(s) 903, 992
Eam1104I CTCTTC 2 cut(s) 389, 508
EarI CTCTTC 2 cut(s) 389, 508
EciI GGCGGA 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 86, 653
Eco147I AGGCCT 1 cut(s) 658
Eco24I GRGCYC 2 cut(s) 96, 886
Eco32I GATATC 2 cut(s) 401, 688
Eco53kI GAGCTC 1 cut(s) 94
Eco72I CACGTG 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 94
EcoRV GATATC 2 cut(s) 401, 688
EcoT14I CCWWGG 2 cut(s) 86, 653
EcoT38I GRGCYC 2 cut(s) 96, 886
ErhI CCWWGG 2 cut(s) 86, 653
FaeI CATG 5 cut(s) 299, 407, 492, 938, 1041
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 2 cut(s) 61, 690
FatI CATG 5 cut(s) 295, 403, 488, 934, 1037
FblI GTMKAC 1 cut(s) 1022
FokI GGATG 2 cut(s) 395, 411
FriOI GRGCYC 2 cut(s) 96, 886
GsaI CCCAGC 3 cut(s) 241, 378, 600
GsuI CTGGAG 1 cut(s) 960
HaeIII GGCC 2 cut(s) 658, 740
HapII CCGG 1 cut(s) 317
Hin1II CATG 5 cut(s) 299, 407, 492, 938, 1041
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 4 cut(s) 221, 699, 827, 971
HpaII CCGG 1 cut(s) 317
HphI GGTGA 2 cut(s) 256, 822
Hpy166II GTNNAC 2 cut(s) 782, 1023
Hpy188I TCNGA 7 cut(s) 226, 478, 499, 704, 850, 992, 997
Hpy188III TCNNGA 3 cut(s) 404, 428, 1038
Hpy8I GTNNAC 2 cut(s) 782, 1023
Hpy99I CGWCG 2 cut(s) 331, 341
HpyAV CCTTC 4 cut(s) 66, 362, 627, 730
HpyCH4III ACNGT 2 cut(s) 762, 774
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 461, 506, 663, 746, 752
HpyF10VI GCNNNNNNNGC 5 cut(s) 306, 309, 312, 458, 746
HpyF3I CTNAG 5 cut(s) 249, 356, 477, 496, 920
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 5 cut(s) 299, 407, 492, 938, 1041
Kzo9I GATC 2 cut(s) 903, 992
LmnI GCTCC 4 cut(s) 91, 114, 263, 290
LweI GCATC 1 cut(s) 940
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 244
MalI GATC 2 cut(s) 905, 994
MbiI CCGCTC 1 cut(s) 109
MboI GATC 2 cut(s) 903, 992
MboII GAAGA 4 cut(s) 406, 525, 612, 869
MhlI GDGCHC 2 cut(s) 96, 886
MluCI AATT 5 cut(s) 22, 153, 158, 456, 943
MluI ACGCGT 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 173, 180
MseI TTAA 1 cut(s) 1011
MslI CAYNNNNRTG 2 cut(s) 294, 487
MspA1I CMGCKG 4 cut(s) 241, 303, 749, 919
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 461
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 5 cut(s) 306, 309, 312, 458, 746
NciI CCSGG 1 cut(s) 317
NdeII GATC 2 cut(s) 903, 992
NlaIII CATG 5 cut(s) 299, 407, 492, 938, 1041
NlaIV GGNNCC 1 cut(s) 818
NmuCI GTSAC 1 cut(s) 244
PagI TCATGA 2 cut(s) 403, 1037
PaqCI CACCTGC 1 cut(s) 793
PceI AGGCCT 1 cut(s) 658
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 4 cut(s) 221, 699, 827, 971
PmaCI CACGTG 1 cut(s) 33
PmlI CACGTG 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 33
PsiI TTATAA 1 cut(s) 389
Psp124BI GAGCTC 1 cut(s) 96
PspCI CACGTG 1 cut(s) 33
PspFI CCCAGC 3 cut(s) 237, 374, 596
PspN4I GGNNCC 1 cut(s) 818
PsrI GAACNNNNNNTAC 2 cut(s) 399, 431
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 503
PvuII CAGCTG 3 cut(s) 241, 749, 919
RseI CAYNNNNRTG 2 cut(s) 294, 487
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 1011
Sau3AI GATC 2 cut(s) 903, 992
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 2 cut(s) 96, 886
SfaNI GCATC 1 cut(s) 940
SfcI CTRYAG 1 cut(s) 750
SmiMI CAYNNNNRTG 2 cut(s) 294, 487
Sse9I AATT 5 cut(s) 22, 153, 158, 456, 943
SseBI AGGCCT 1 cut(s) 658
SsiI CCGC 3 cut(s) 107, 303, 380
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 658
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 2 cut(s) 86, 653
TaaI ACNGT 2 cut(s) 762, 774
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 339, 886
TasI AATT 5 cut(s) 22, 153, 158, 456, 943
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 4 cut(s) 221, 699, 827, 971
Tru1I TTAA 1 cut(s) 1011
Tru9I TTAA 1 cut(s) 1011
TscAI CASTG 3 cut(s) 607, 777, 984
TseFI GTSAC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 4 cut(s) 413, 420, 477, 1026
TspRI CASTG 3 cut(s) 607, 777, 984
XapI RAATTY 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.