Rh4AG250100

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014068
Physical Location & Seq
Forward (+)
56215992 .. 56217491
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG250100.1

Sequence Viewer

Length: 831 bp
ATGGAACATGAATCTGACTTCAAATCGCAGTTGGTGACAGAGATTTGCTCCATTTCGAAATTCTCCATTGCATGTTCTCATAGGCATTGCACCAGCCCTGTTCGATCTTCTTCACCTTTCGTCGATTGGTATCGTCTTCTTGGGGTGGAAGAAACAGCTGGGATTGATGTTATAAGAAAGAGATACCATGAACTTGCTTTACAACTTCACCCAGATAAGAACAAGCATGCCAAGGCTGAAGTTGCCTTCAAACTTGTCTCAGAGGCATATTCATGCCTCTCTGATCATGAAAATAGAAGAGCTTTTGACTTGGAGAGATGGAGCAAGTTCTGCTTTGAGTGTGGTACAATCCCCTACACAACCTACAAGACTCCCAGCAATGCCAAGGCTTCAGAACACAAGGGTTGGAATCCCACAAGCAGTTCGAGGTCTTGTAAAGTTATCAAGGGCCTGAAAGATATGAGAAACCGGTTTAGAGAAGAGGCAAGAGTGATCGAAAACTGTCTGAGGGCTAATAATGCAGCATCAAGAAATGAATCCTCGCTCTTCAGTCCAACTTCTTATATGTTTCAGAGCAATTCTAATCCTAGGTTGAGCAGGGAAACCCCTGTTTTCAACCCATCTGATCATGTGGTTCAAGGATATCCCCATGTCAGGACTCGAATTTACGACAAAGATAAATCTCGGAGCTTTTGGTGCTTGAGAACAGGGCATCATATATTGAATTATGAACAAGGAAGGGCTAGTAGGGCTACATATGATTCTCCAGTATTTGAGGTCAGATCAGAAAGGGGAATTTTAAAGAGTAGATCTACTTGTGTTCGTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

276

Amino Acids

31.84

Weight (kDa)

9.36

Isoelectric Point (pI)

67.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 42 - 103 1.7e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06778
fragaria_vesca FvH4_4g18890
malus_domestica MD13G1162300.v1.1 MD16G1162100.v1.1
prunus_persica Prupe.1G129600_v2.0.a1
pyrus_communis pycom13g13850 pycom16g13750
rosa_chinensis RchiOBHm_Chr4g0423791
rosa_multiflora Rmu_co8479069.1_g000001
rosa_roxburghii Rroxscaffold_5G00365900
rosa_rugosa Rorug04G0190600 Rorug04G0190700
rosa_samantha Rh4AG250100 Rh4BG255300 Rh4CG263300 Rh4DG249600
rosa_wichuraiana Rw4G021410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 173
AcsI RAATTY 3 cut(s) 59, 663, 795
AcuI CTGAAG 3 cut(s) 258, 375, 532
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 1 cut(s) 654
AgeI ACCGGT 1 cut(s) 468
AgsI TTSAA 5 cut(s) 22, 250, 616, 638, 724
AluBI AGCT 3 cut(s) 158, 302, 690
AluI AGCT 3 cut(s) 158, 302, 690
Alw26I GTCTC 1 cut(s) 262
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 3 cut(s) 59, 663, 795
AsiGI ACCGGT 1 cut(s) 468
AspA2I CCTAGG 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 3 cut(s) 46, 105, 200
AsuII TTCGAA 1 cut(s) 56
AvrII CCTAGG 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 533
BccI CCATC 2 cut(s) 312, 628
BclI TGATCA 2 cut(s) 283, 625
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 2 cut(s) 588, 744
BglII AGATCT 1 cut(s) 809
BisI GCNGC 1 cut(s) 522
BlnI CCTAGG 1 cut(s) 587
BlsI GCNGC 1 cut(s) 523
BmgT120I GGNCC 1 cut(s) 448
BmsI GCATC 2 cut(s) 533, 721
BpiI GAAGAC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 721
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 3 cut(s) 231, 384, 587
BsaWI WCCGGW 1 cut(s) 468
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 1 cut(s) 767
Bse3DI GCAATG 3 cut(s) 66, 85, 385
Bse8I GATNNNNATC 1 cut(s) 129
BseDI CCNNGG 3 cut(s) 231, 384, 587
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMI GCAATG 3 cut(s) 66, 85, 385
BseMII CTCAG 2 cut(s) 273, 497
BseNI ACTGG 1 cut(s) 767
BseXI GCAGC 1 cut(s) 533
BseYI CCCAGC 2 cut(s) 158, 374
BshFI GGCC 1 cut(s) 450
BshTI ACCGGT 1 cut(s) 468
BsiSI CCGG 1 cut(s) 469
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 1 cut(s) 262
BsnI GGCC 1 cut(s) 450
Bsp119I TTCGAA 1 cut(s) 56
Bsp143I GATC 6 cut(s) 104, 283, 492, 625, 782, 809
BspANI GGCC 1 cut(s) 450
BspCNI CTCAG 2 cut(s) 272, 498
BspHI TCATGA 1 cut(s) 286
BspQI GCTCTTC 2 cut(s) 292, 551
BspT104I TTCGAA 1 cut(s) 56
BsrDI GCAATG 3 cut(s) 66, 85, 385
BsrFI RCCGGY 1 cut(s) 468
BsrI ACTGG 1 cut(s) 767
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 231, 384, 587
BssMI GATC 6 cut(s) 104, 283, 492, 625, 782, 809
BssT1I CCWWGG 3 cut(s) 231, 384, 587
Bst4CI ACNGT 1 cut(s) 503
Bst6I CTCTTC 3 cut(s) 292, 474, 551
BstAPI GCANNNNNTGC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 2 cut(s) 259, 506
BstKTI GATC 6 cut(s) 107, 286, 495, 628, 785, 812
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 6 cut(s) 104, 283, 492, 625, 782, 809
BstMWI GCNNNNNNNGC 5 cut(s) 242, 330, 518, 696, 749
BstNSI RCATGY 2 cut(s) 75, 230
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 809
BstYI RGATCY 1 cut(s) 809
BsuRI GGCC 1 cut(s) 450
Cac8I GCNNGC 1 cut(s) 228
CciI TCATGA 1 cut(s) 286
Cfr10I RCCGGY 1 cut(s) 468
Cfr13I GGNCC 1 cut(s) 448
Csp6I GTAC 1 cut(s) 345
CspAI ACCGGT 1 cut(s) 468
CviAII CATG 8 cut(s) 8, 72, 188, 227, 273, 287, 629, 650
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 2 cut(s) 259, 506
DpnI GATC 6 cut(s) 106, 285, 494, 627, 784, 811
DpnII GATC 6 cut(s) 104, 283, 492, 625, 782, 809
DraI TTTAAA 1 cut(s) 801
Eam1104I CTCTTC 3 cut(s) 292, 474, 551
EarI CTCTTC 3 cut(s) 292, 474, 551
Eco130I CCWWGG 3 cut(s) 231, 384, 587
Eco32I GATATC 1 cut(s) 644
Eco57I CTGAAG 3 cut(s) 258, 375, 532
EcoO109I RGGNCCY 1 cut(s) 448
EcoRV GATATC 1 cut(s) 644
EcoT14I CCWWGG 3 cut(s) 231, 384, 587
ErhI CCWWGG 3 cut(s) 231, 384, 587
FaeI CATG 8 cut(s) 11, 75, 191, 230, 276, 290, 632, 653
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 8 cut(s) 7, 71, 187, 226, 272, 286, 628, 649
FauNDI CATATG 1 cut(s) 757
FbaI TGATCA 2 cut(s) 283, 625
Fnu4HI GCNGC 1 cut(s) 522
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 2 cut(s) 588, 744
GluI GCNGC 1 cut(s) 522
GsaI CCCAGC 2 cut(s) 162, 378
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 1 cut(s) 450
HapII CCGG 1 cut(s) 469
Hin1II CATG 8 cut(s) 11, 75, 191, 230, 276, 290, 632, 653
HinfI GANTC 6 cut(s) 11, 370, 409, 536, 658, 761
HpaII CCGG 1 cut(s) 469
HphI GGTGA 3 cut(s) 46, 105, 200
Hpy188III TCNNGA 3 cut(s) 287, 528, 655
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 256, 732
HpyCH4III ACNGT 1 cut(s) 503
HpyCH4V TGCA 3 cut(s) 71, 90, 521
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 330, 518, 696, 749
HpyF3I CTNAG 2 cut(s) 259, 506
Hsp92II CATG 8 cut(s) 11, 75, 191, 230, 276, 290, 632, 653
Ksp22I TGATCA 2 cut(s) 283, 625
Kzo9I GATC 6 cut(s) 104, 283, 492, 625, 782, 809
LguI GCTCTTC 2 cut(s) 292, 551
LmnI GCTCC 3 cut(s) 53, 321, 687
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 2 cut(s) 533, 721
MaeI CTAG 2 cut(s) 588, 744
MaeIII GTNAC 1 cut(s) 34
MalI GATC 6 cut(s) 106, 285, 494, 627, 784, 811
MboI GATC 6 cut(s) 104, 283, 492, 625, 782, 809
MboII GAAGA 7 cut(s) 99, 102, 128, 161, 309, 491, 538
MflI RGATCY 1 cut(s) 809
MluCI AATT 5 cut(s) 59, 577, 663, 724, 795
MlyI GAGTC 2 cut(s) 364, 652
MmeI TCCRAC 2 cut(s) 386, 578
MnlI CCTC 7 cut(s) 256, 287, 420, 475, 501, 550, 769
MseI TTAA 1 cut(s) 800
MslI CAYNNNNRTG 1 cut(s) 271
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 1 cut(s) 469
MwoI GCNNNNNNNGC 5 cut(s) 242, 330, 518, 696, 749
NdeI CATATG 1 cut(s) 757
NdeII GATC 6 cut(s) 104, 283, 492, 625, 782, 809
NlaIII CATG 8 cut(s) 11, 75, 191, 230, 276, 290, 632, 653
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 2 cut(s) 75, 230
NspV TTCGAA 1 cut(s) 56
PaeI GCATGC 1 cut(s) 230
PagI TCATGA 1 cut(s) 286
PciSI GCTCTTC 2 cut(s) 292, 551
PfeI GAWTC 4 cut(s) 11, 409, 536, 761
PinAI ACCGGT 1 cut(s) 468
PkrI GCNGC 1 cut(s) 523
PleI GAGTC 2 cut(s) 364, 652
PpsI GAGTC 2 cut(s) 364, 652
PsiI TTATAA 1 cut(s) 173
PspFI CCCAGC 2 cut(s) 158, 374
PspPI GGNCC 1 cut(s) 448
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PsuI RGATCY 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
RseI CAYNNNNRTG 1 cut(s) 271
SapI GCTCTTC 2 cut(s) 292, 551
SaqAI TTAA 1 cut(s) 800
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 6 cut(s) 104, 283, 492, 625, 782, 809
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 2 cut(s) 364, 652
SetI ASST 8 cut(s) 118, 160, 304, 365, 431, 593, 692, 780
SfaNI GCATC 2 cut(s) 533, 721
SfuI TTCGAA 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 271
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
SphI GCATGC 1 cut(s) 230
Sse9I AATT 5 cut(s) 59, 577, 663, 724, 795
SspMI CTAG 2 cut(s) 588, 744
StyI CCWWGG 3 cut(s) 231, 384, 587
TaaI ACNGT 1 cut(s) 503
TaqI TCGA 6 cut(s) 56, 103, 123, 425, 495, 661
TasI AATT 5 cut(s) 59, 577, 663, 724, 795
TfiI GAWTC 4 cut(s) 11, 409, 536, 761
Tru1I TTAA 1 cut(s) 800
Tru9I TTAA 1 cut(s) 800
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 6 cut(s) 24, 204, 261, 303, 549, 744
XapI RAATTY 3 cut(s) 59, 663, 795
XceI RCATGY 2 cut(s) 75, 230
XmaJI CCTAGG 1 cut(s) 587
XspI CTAG 2 cut(s) 588, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.