Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007897

Physical Location & Seq

Genomic Coordinates Reverse (-)

13191950 .. 13194621

Gene Structure 2672 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1165700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1164 bp

ATGAGAGCTCCTCAAATGGGGGATCAAGTTGACGAATTTCAGCCACTTTGTGAAGTGCAAAGTGACAAAGCAACCATCGAAATAACAAGCCGTTATCTGGGGAAAGTTTCTCAGCTTCTCTATGTTCCAGGTGACATCGTCAAGGTTGGAGAAATTCTTTTAAAGATGGCCGTCGAGGAATCTCAGGTACCAAAGCAGATATGTGAGAGTTTGGAAAACACTAAGTCACTAGATTCTGAGCTTAACACACATAACATAGGCGGAGTTTTGTCTACACCTCCTGTTCGGAGCCTTGCAAAGCAATATGATATAGATATAAATGAAGTCTGTGGAACTGGTAAAGATGGGAGAGTGTTACGAGCAGATGTCCTTAAATATGCTGCCCAGAAGGGAATCATTGAAGTTCCTTCTGCCTCCTTGAGTGCTAGTCCTGAGAAAGCTTTTGGAGGAAAAGAGACTTGTGCACATACATCAGCTGAATCGGGATGGAATTATGAGGATAAGACAGTTACCCTGAGCATGGCTGCAAAAGTTCCACATTTTCATTATGTTGAGGGTATAAAGTGTGATGCACTTGTGGAGCTGAAAAATTCTTTCCAAAGTAATAATACCGATTCAAATGTCAAGCACACTTTCCTTCCATTATTGATGAAGACTCTCTCAATGGCCATGAACAAATATCCCCTGATGAATAGTAGCTTCAATGAAGAGTCACTCGAGGTCACCCTCAAAGGTTCCCACAATATCGGAATTGCCATGGCTACTCCATATGGTCTAGTTGTACCAATCATAAAGAATGTTCAGTCTCTTTCTATTTTGGAGATAACAATGGAACTTTCACGGTTACTACAACTTGCATTGGAAAACAAGCTCAAGCGTGAAGATATATCTGACGGAACAATAACTTTAAGTACTATTGGAGCAATTGGTGGGAAATATGGTTCGCCTCTTCTCAACTTACCTGAGGTTGCCATTATTGCAATTGGACGAATGCAGAAGGTTCCACGATTTGCAGATGACGGAACTTTACATCCTGTCTCAGTTATGACGGTCAATATAGGCGCAGATCATAGAGTGCTGGATGGAGCAACTGTTGCCAGGTTCTGCAACGAATGGAAACAATTTATAGAGAATCCAGAGCTGCTGATGTTGCGTATGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004147 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006950 GO:0007154 GO:0008150 GO:0008270 GO:0009267 GO:0009295 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009987 GO:0009991 GO:0010033 GO:0016407 GO:0016417 GO:0016740 GO:0016746 GO:0016747 GO:0019899 GO:0030062 GO:0030523 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0032991 GO:0033554 GO:0034285 GO:0042221 GO:0042594 GO:0042645 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043617 GO:0044389 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0070013 GO:0071496 GO:0098798 GO:1901700 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00280 ko00640 ko01100 ko01110 ko01130 map00280 map00640 map01100 map01110 map01130

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

388

Amino Acids

42.42

Weight (kDa)

5.71

Isoelectric Point (pI)

44.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	6 - 56	2.3e-11	Biotin-requiring enzyme
E3_binding	PF02817	89 - 124	7.5e-12	e3 binding domain
2-oxoacid_dh	PF00198	173 - 384	2.3e-71	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007897)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06850 AT3G06850
fragaria_vesca	FvH4_4g18561 FvH4_4g18561 FvH4_4g18561
malus_domestica	MD13G1165700.v1.1
prunus_persica	Prupe.1G132500_v2.0.a1
pyrus_communis	pycom13g14180 pycom16g14020
rosa_chinensis	RchiOBHm_Chr4g0423151
rosa_multiflora	Rmu_sc0000744.1_g000002 Rmu_sc0000744.1_g000004 Rmu_sc0008829.1_g000001 Rmu_sc0027756.1_g000002
rosa_roxburghii	Rroxscaffold_5G00365320
rosa_rugosa	Rorug04G0184700 Rorug04G0184800 Rorug04G0184800
rosa_samantha	Rh4AG244200 Rh4BG249400 Rh4CG258500 Rh4DG244100 Rh4DG244200
rosa_wichuraiana	Rw4G021040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	187
AccB1I	GGYRCC	1 cut(s)	187
AccI	GTMKAC	1 cut(s)	272
AciI	CCGC	1 cut(s)	261
AclWI	GGATC	1 cut(s)	30
AcoI	YGGCCR	2 cut(s)	168, 666
AcsI	RAATTY	3 cut(s)	35, 153, 589
AdeI	CACNNNGTG	1 cut(s)	50
AfaI	GTAC	3 cut(s)	189, 783, 913
AfiI	CCNNNNNNNGG	3 cut(s)	17, 97, 520
AgsI	TTSAA	3 cut(s)	401, 618, 703
AjnI	CCWGG	2 cut(s)	127, 1097
AloI	GAACNNNNNNTCC	4 cut(s)	925, 957, 1015, 1047
AluBI	AGCT	9 cut(s)	8, 115, 241, 440, 476, 583, 699, 871, 1141
AluI	AGCT	9 cut(s)	8, 115, 241, 440, 476, 583, 699, 871, 1141
Alw21I	GWGCWC	2 cut(s)	10, 466
Alw26I	GTCTC	3 cut(s)	449, 810, 1042
Alw44I	GTGCAC	1 cut(s)	462
AlwI	GGATC	1 cut(s)	30
AlwNI	CAGNNNCTG	1 cut(s)	1104
Ama87I	CYCGRG	1 cut(s)	716
AoxI	GGCC	2 cut(s)	168, 666
ApaLI	GTGCAC	1 cut(s)	462
ApeKI	GCWGC	3 cut(s)	380, 524, 1141
ApoI	RAATTY	3 cut(s)	35, 153, 589
Asp718I	GGTACC	1 cut(s)	187
AspLEI	GCGC	1 cut(s)	1064
AsuHPI	GGTGA	2 cut(s)	143, 715
AvaI	CYCGRG	1 cut(s)	716
AxyI	CCTNAGG	1 cut(s)	963
BaeGI	GKGCMC	1 cut(s)	466
BalI	TGGCCA	1 cut(s)	668
BanI	GGYRCC	1 cut(s)	187
BanII	GRGCYC	1 cut(s)	10
BbsI	GAAGAC	1 cut(s)	659
Bbv12I	GWGCWC	2 cut(s)	10, 466
BbvI	GCAGC	3 cut(s)	367, 511, 1128
BccI	CCATC	5 cut(s)	83, 160, 338, 480, 1076
BceAI	ACGGC	2 cut(s)	75, 155
BciT130I	CCWGG	2 cut(s)	129, 1099
BcoDI	GTCTC	3 cut(s)	449, 810, 1042
BfaI	CTAG	3 cut(s)	230, 426, 776
BisI	GCNGC	3 cut(s)	381, 525, 1142
BlsI	GCNGC	3 cut(s)	382, 526, 1143
BmcAI	AGTACT	1 cut(s)	913
Bme1390I	CCNGG	2 cut(s)	129, 1099
BmeT110I	CYCGRG	1 cut(s)	716
BmiI	GGNNCC	4 cut(s)	189, 290, 736, 1002
BmrFI	CCNGG	2 cut(s)	129, 1099
BmsI	GCATC	1 cut(s)	559
BpiI	GAAGAC	1 cut(s)	659
BplI	GAGNNNNNCTC	1 cut(s)	27
Bpu10I	CCTNAGC	1 cut(s)	515
BpuEI	CTTGAG	2 cut(s)	439, 857
BsaJI	CCNNGG	1 cut(s)	756
BsaXI	ACNNNNNCTCC	2 cut(s)	1077, 1107
Bsc4I	CCNNNNNNNGG	3 cut(s)	17, 97, 520
Bse1I	ACTGG	1 cut(s)	340
Bse21I	CCTNAGG	1 cut(s)	963
BseBI	CCWGG	2 cut(s)	129, 1099
BseDI	CCNNGG	1 cut(s)	756
BseGI	GGATG	3 cut(s)	491, 1030, 1087
BseLI	CCNNNNNNNGG	3 cut(s)	17, 97, 520
BseMII	CTCAG	7 cut(s)	125, 197, 228, 423, 506, 954, 1053
BseNI	ACTGG	1 cut(s)	340
BseSI	GKGCMC	1 cut(s)	466
BseXI	GCAGC	3 cut(s)	367, 511, 1128
BshFI	GGCC	2 cut(s)	170, 668
BshNI	GGYRCC	1 cut(s)	187
BsiHKAI	GWGCWC	2 cut(s)	10, 466
BsiHKCI	CYCGRG	1 cut(s)	716
BslI	CCNNNNNNNGG	3 cut(s)	17, 97, 520
BsmAI	GTCTC	3 cut(s)	449, 810, 1042
BsmI	GAATGC	1 cut(s)	996
BsnI	GGCC	2 cut(s)	170, 668
BsoBI	CYCGRG	1 cut(s)	716
Bsp1286I	GDGCHC	2 cut(s)	10, 466
Bsp143I	GATC	2 cut(s)	22, 1066
Bsp19I	CCATGG	1 cut(s)	756
BspACI	CCGC	1 cut(s)	261
BspANI	GGCC	2 cut(s)	170, 668
BspCNI	CTCAG	7 cut(s)	124, 196, 229, 424, 507, 955, 1052
BspLI	GGNNCC	4 cut(s)	189, 290, 736, 1002
BspPI	GGATC	1 cut(s)	30
BspT107I	GGYRCC	1 cut(s)	187
BsrI	ACTGG	1 cut(s)	340
BssECI	CCNNGG	1 cut(s)	756
BssMI	GATC	2 cut(s)	22, 1066
BssT1I	CCWWGG	1 cut(s)	756
Bst2UI	CCWGG	2 cut(s)	129, 1099
Bst4CI	ACNGT	4 cut(s)	508, 843, 1051, 1093
Bst6I	CTCTTC	2 cut(s)	702, 954
BstAPI	GCANNNNNTGC	1 cut(s)	1094
BstDEI	CTNAG	8 cut(s)	111, 183, 222, 237, 432, 515, 963, 1039
BstDSI	CCRYGG	1 cut(s)	756
BstEII	GGTNACC	1 cut(s)	721
BstF5I	GGATG	3 cut(s)	491, 1030, 1087
BstHHI	GCGC	1 cut(s)	1064
BstKTI	GATC	2 cut(s)	25, 1069
BstMAI	GTCTC	3 cut(s)	449, 810, 1042
BstMBI	GATC	2 cut(s)	22, 1066
BstMWI	GCNNNNNNNGC	3 cut(s)	977, 1094, 1150
BstNI	CCWGG	2 cut(s)	129, 1099
BstPI	GGTNACC	1 cut(s)	721
BstSCI	CCNGG	2 cut(s)	127, 1097
BstSLI	GKGCMC	1 cut(s)	466
BstV1I	GCAGC	3 cut(s)	367, 511, 1128
BstV2I	GAAGAC	1 cut(s)	659
Bsu36I	CCTNAGG	1 cut(s)	963
BsuRI	GGCC	2 cut(s)	170, 668
BtgI	CCRYGG	1 cut(s)	756
BtsCI	GGATG	3 cut(s)	491, 1030, 1087
CaiI	CAGNNNCTG	1 cut(s)	1104
CfoI	GCGC	1 cut(s)	1064
Csp6I	GTAC	3 cut(s)	188, 782, 912
CviAII	CATG	3 cut(s)	520, 670, 757
CviQI	GTAC	3 cut(s)	188, 782, 912
DdeI	CTNAG	8 cut(s)	111, 183, 222, 237, 432, 515, 963, 1039
DpnI	GATC	2 cut(s)	24, 1068
DpnII	GATC	2 cut(s)	22, 1066
DraI	TTTAAA	1 cut(s)	162
DraIII	CACNNNGTG	1 cut(s)	50
EaeI	YGGCCR	2 cut(s)	168, 666
Eam1104I	CTCTTC	2 cut(s)	702, 954
EarI	CTCTTC	2 cut(s)	702, 954
EciI	GGCGGA	1 cut(s)	276
Ecl136II	GAGCTC	1 cut(s)	8
Eco130I	CCWWGG	1 cut(s)	756
Eco24I	GRGCYC	1 cut(s)	10
Eco53kI	GAGCTC	1 cut(s)	8
Eco81I	CCTNAGG	1 cut(s)	963
Eco88I	CYCGRG	1 cut(s)	716
Eco91I	GGTNACC	1 cut(s)	721
EcoICRI	GAGCTC	1 cut(s)	8
EcoO65I	GGTNACC	1 cut(s)	721
EcoRII	CCWGG	2 cut(s)	127, 1097
EcoT14I	CCWWGG	1 cut(s)	756
EcoT38I	GRGCYC	1 cut(s)	10
ErhI	CCWWGG	1 cut(s)	756
FaeI	CATG	3 cut(s)	523, 673, 760
FatI	CATG	3 cut(s)	519, 669, 756
FauNDI	CATATG	1 cut(s)	769
FblI	GTMKAC	1 cut(s)	272
Fnu4HI	GCNGC	3 cut(s)	381, 525, 1142
FokI	GGATG	3 cut(s)	498, 1017, 1094
FriOI	GRGCYC	1 cut(s)	10
Fsp4HI	GCNGC	3 cut(s)	381, 525, 1142
FspBI	CTAG	3 cut(s)	230, 426, 776
GlaI	GCGC	1 cut(s)	1063
GluI	GCNGC	3 cut(s)	381, 525, 1142
HaeIII	GGCC	2 cut(s)	170, 668
HhaI	GCGC	1 cut(s)	1064
Hin1II	CATG	3 cut(s)	523, 673, 760
Hin6I	GCGC	1 cut(s)	1062
HinP1I	GCGC	1 cut(s)	1062
HincII	GTYRAC	1 cut(s)	31
HindII	GTYRAC	1 cut(s)	31
HindIII	AAGCTT	1 cut(s)	438
HinfI	GANTC	8 cut(s)	179, 233, 393, 479, 614, 655, 710, 1132
HphI	GGTGA	2 cut(s)	143, 715
Hpy166II	GTNNAC	3 cut(s)	31, 273, 464
Hpy188I	TCNGA	4 cut(s)	238, 288, 749, 892
Hpy188III	TCNNGA	3 cut(s)	431, 483, 1136
Hpy8I	GTNNAC	3 cut(s)	31, 273, 464
Hpy99I	CGWCG	1 cut(s)	176
HpyAV	CCTTC	4 cut(s)	382, 417, 647, 991
HpyCH4III	ACNGT	4 cut(s)	508, 843, 1051, 1093
HpyF10VI	GCNNNNNNNGC	3 cut(s)	977, 1094, 1150
HpyF3I	CTNAG	8 cut(s)	111, 183, 222, 237, 432, 515, 963, 1039
Hsp92II	CATG	3 cut(s)	523, 673, 760
HspAI	GCGC	1 cut(s)	1062
KpnI	GGTACC	1 cut(s)	191
Kzo9I	GATC	2 cut(s)	22, 1066
LmnI	GCTCC	5 cut(s)	13, 288, 580, 920, 1085
Lsp1109I	GCAGC	3 cut(s)	367, 511, 1128
LweI	GCATC	1 cut(s)	559
MaeI	CTAG	3 cut(s)	230, 426, 776
MaeIII	GTNAC	8 cut(s)	62, 131, 225, 354, 508, 711, 721, 843
MalI	GATC	2 cut(s)	24, 1068
MboI	GATC	2 cut(s)	22, 1066
MboII	GAAGA	4 cut(s)	664, 719, 893, 941
MfeI	CAATTG	2 cut(s)	924, 981
MhlI	GDGCHC	2 cut(s)	10, 466
MlsI	TGGCCA	1 cut(s)	668
MluCI	AATT	8 cut(s)	35, 153, 490, 589, 750, 924, 981, 1121
MluNI	TGGCCA	1 cut(s)	668
MlyI	GAGTC	2 cut(s)	649, 719
MmeI	TCCRAC	1 cut(s)	127
Mox20I	TGGCCA	1 cut(s)	668
MscI	TGGCCA	1 cut(s)	668
MseI	TTAA	4 cut(s)	161, 243, 372, 908
Msp20I	TGGCCA	1 cut(s)	668
MspA1I	CMGCKG	1 cut(s)	476
MspR9I	CCNGG	2 cut(s)	129, 1099
MunI	CAATTG	2 cut(s)	924, 981
Mva1269I	GAATGC	1 cut(s)	996
MvaI	CCWGG	2 cut(s)	129, 1099
MwoI	GCNNNNNNNGC	3 cut(s)	977, 1094, 1150
NcoI	CCATGG	1 cut(s)	756
NdeI	CATATG	1 cut(s)	769
NdeII	GATC	2 cut(s)	22, 1066
NlaIII	CATG	3 cut(s)	523, 673, 760
NlaIV	GGNNCC	4 cut(s)	189, 290, 736, 1002
NmuCI	GTSAC	5 cut(s)	62, 131, 225, 711, 721
PaeR7I	CTCGAG	1 cut(s)	716
PctI	GAATGC	1 cut(s)	996
PfeI	GAWTC	6 cut(s)	179, 233, 393, 479, 614, 1132
PflFI	GACNNNGTC	1 cut(s)	137
PkrI	GCNGC	3 cut(s)	382, 526, 1143
PleI	GAGTC	2 cut(s)	649, 718
PpsI	GAGTC	2 cut(s)	649, 718
Psp124BI	GAGCTC	1 cut(s)	10
Psp6I	CCWGG	2 cut(s)	127, 1097
PspEI	GGTNACC	1 cut(s)	721
PspGI	CCWGG	2 cut(s)	127, 1097
PspN4I	GGNNCC	4 cut(s)	189, 290, 736, 1002
PspXI	VCTCGAGB	1 cut(s)	716
PstNI	CAGNNNCTG	1 cut(s)	1104
PsyI	GACNNNGTC	1 cut(s)	137
PvuII	CAGCTG	1 cut(s)	476
RsaI	GTAC	3 cut(s)	189, 783, 913
RsaNI	GTAC	3 cut(s)	188, 782, 912
SacI	GAGCTC	1 cut(s)	10
SaqAI	TTAA	4 cut(s)	161, 243, 372, 908
SatI	GCNGC	3 cut(s)	381, 525, 1142
Sau3AI	GATC	2 cut(s)	22, 1066
ScaI	AGTACT	1 cut(s)	913
SchI	GAGTC	2 cut(s)	649, 719
ScrFI	CCNGG	2 cut(s)	129, 1099
SduI	GDGCHC	2 cut(s)	10, 466
SfaNI	GCATC	1 cut(s)	559
Sfr274I	CTCGAG	1 cut(s)	716
SlaI	CTCGAG	1 cut(s)	716
SmlI	CTYRAG	3 cut(s)	418, 716, 872
SmoI	CTYRAG	3 cut(s)	418, 716, 872
Sse9I	AATT	8 cut(s)	35, 153, 490, 589, 750, 924, 981, 1121
SsiI	CCGC	1 cut(s)	261
SspMI	CTAG	3 cut(s)	230, 426, 776
SstI	GAGCTC	1 cut(s)	10
StyD4I	CCNGG	2 cut(s)	127, 1097
StyI	CCWWGG	1 cut(s)	756
TaaI	ACNGT	4 cut(s)	508, 843, 1051, 1093
TaqI	TCGA	3 cut(s)	78, 174, 717
TasI	AATT	8 cut(s)	35, 153, 490, 589, 750, 924, 981, 1121
TatI	WGTACW	1 cut(s)	911
TfiI	GAWTC	6 cut(s)	179, 233, 393, 479, 614, 1132
Tru1I	TTAA	4 cut(s)	161, 243, 372, 908
Tru9I	TTAA	4 cut(s)	161, 243, 372, 908
TseFI	GTSAC	5 cut(s)	62, 131, 225, 711, 721
TseI	GCWGC	3 cut(s)	380, 524, 1141
Tsp45I	GTSAC	5 cut(s)	62, 131, 225, 711, 721
TspDTI	ATGAA	6 cut(s)	336, 533, 665, 686, 704, 720
TspGWI	ACGGA	2 cut(s)	909, 1035
Tth111I	GACNNNGTC	1 cut(s)	137
VneI	GTGCAC	1 cut(s)	462
XapI	RAATTY	3 cut(s)	35, 153, 589
XhoI	CTCGAG	1 cut(s)	716
XmiI	GTMKAC	1 cut(s)	272
XspI	CTAG	3 cut(s)	230, 426, 776
ZrmI	AGTACT	1 cut(s)	913

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.