Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008859

Physical Location & Seq

Genomic Coordinates Reverse (-)

14746755 .. 14751104

Gene Structure 4350 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1177600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2772 bp

ATGGTGGCCAAGGGCAGTAGTAGTTGCACCACCCAAGTCTCTTCCTCAATAGCTGTTCAAGAAAAGGGAAGTAGGAATAAGAGGAAGTTCCGGGCTGATCCGCCTTTAGGTGACCCGAATAAAATTATTCCCTTATCTCAGACTGAATGCACAAGCTATGAGTTTTCAGCTGAAAAATTCGAAATCACGCAGAGTCATGGACAAATTGGTGTCTGTGACCTGTGCAGTGTTAATCAAGACCATTCTGATGGGTTGAAACTCGACCTTGGATTGTCGTCTAGTGCTGTTGTTCCATCCGAGGGTAGCCCAAGCTGGCCCAGAGAGGAACTAGAGGCAGAGTTTCAGGATGCCGATTGGAGTGACCTCACAGAAAACCAACTTGAAGAGCTTGTTCTAAGCAATTTGGACATGATTTTCAAAGGTGCAATAAAAAAGATTGTTGCTTGCGGTTACACTGAAGAAGTTGCTACAAAGGCTGTTTTGCGGTCTGGTCTTTGTTATGGGTGTAAAGACACCGTGTCTAATATAGTGGATAATACTTTAGTTTATCTAAGAAGTGGCCAAGAAATTGATCCTTCGAGGGAACACTGTTTTGAAGATCTACAGCAGCTTGAGAAGTACATATTGGCAGAACTGGTTTGTGTTCTTCAAGAGGTTAGGCCTTTTTTCAGCACTGGTGATGCAATGTGGTGCTTGTTGATTTGTGATATGAATGTCTCTCATGCTTGTGCAATGGATGGTGATCCCTTGAATAGTTTCATTAGTGATGGAGCTTCAAATGGAAGTTCCTCCGTCCCGAATCAACCCCAGTCAAAAACAGAAACCAAAAGTTCGGAATTGAATCTTCTGAATCCTTCCAAGCCAGTTCCTGGTTCTCATAGTTCACAGTCTGAGACACCTATGATTGCAGGTGGGGTTTCAAACATAGCTAAACTGAAAAATTCCCTTGTTCATAGTGCATCGTTGTCTGAGAAAGAGGGTGCAAAGTCCACATCTGAGAATGGGGATAAATCTTTTGGTGCATCTGGAACATTTCAATCTCCTGTGGTAGAGGAAAAGATTTTAAGTAGTCGAAAGGTTCATTCTGTTACCACCAAGAGAGAATACATGCTTCGGCACAAGTCACTTCACTTGGAAAAAAGTTACCGGACTTATGGATGTAAAGGGTCTTCAAGAACGGGGAAGCTGAGTGGTTTGGGTGGTTTAATCTTGGATAAGAAACTAAAATCTGTGTCAGACTCTACCACTGTTAATTTGAAGAATGCTTCCTTGAAGATAAGCAAGGCTATGGGAGTTGATGTGCCTCAAGAAAGTGGAAACAATAATCTTTCTGCCAATGCCGGGCCTTCTTCTCCTAGAGCATTTAACCTGGATGTTGAAAATACTGCTTCTGTTTTGCCTAAGAACAGTGTCACGTCCATGTTACCTTCTGTTTGTACAACAGCACTACCTTCTGTCGGTACTTCAACAGCATTACCTTCTGTCAATACTTCAACGGCATTACCTGTAGTCAATACTGCCGCAGCATTACCTTCAGCAAATACTACGCCTGCATTATCAGTTGCTGATACTGAGCTTTCCCTTTCTCTACATCCAAAAAGCATTTCCAATCCAGTTCCTATTAGCTGTCACTCTGATGCAACTAATTCTGTTTTTGCTGGGATACCGTATGATAAATCCTTGGGGCAGTGGGTCCCCCGGGACAAGAAAGATGAAATGATTTTAAAGTTGGTTCCGAGGGCACGGGACTTGCAAAATCAGCTCCAAGAGTGGACAGAGTGGGCAAATCAGAAGGTTATGCAGGCTGCGCGTAGATTGAGTAAGGACAAGGCTGAACTTAAGTCATTGAGGCAAGAGAAAGAAGAAGTTGAGCGGCTGAAGAAAGAGAAACAAACACTGGAAGAAAACACCATGAAGAAGCTTTCTGAGATGGAAAATGCTCTCTGCAAGGCTAGTAGCCAGGTTGAACGGGCAAATTCTTCCGTCCGGAGGCTTGAGGTGGAGAATGCTGCCTTGAGGCAGGATATGGAGGCTGCTAAAGTACGTGCTGCAGAGTCTGCTGCAAGCTGTCAGGATGTATCTAAAAGGGAGAAGAAGACACTGATGAAGTTTCAGTCATGGGAGAAGCAGAAAACCATGTTCAGTGAAGAACTTGCGACTGAAAAACGCAAGTTAAAACAGCTGCTACAGGAACTAGAACAAGCCAAAGATCTCCAGGAACAATTGGAGGCTAGATGGCAACAGGAAAAAAAATCAAAGGCAGAAGTACTTGAGCAGGTCAGTTCGATAAGAAAAGAACGGGAACAGATAGAGGCTTCAACAAAATCCAAGGAGGATATGATTAACCTGAAAGCAGAAAACAATCTGCAGAAGTACAAAGATGATATTCAGAAACTCGAAAAAGAAATCTCTCAATTGAGACACAAGAGTGATTCTTCAAAAATTGCTGCGCTGCGGAGGGGCATTGACGGGGGCTACGCCAGCAAAGTAACAGACATCGAAAATGGCCTGGATCACAAGGGGTCCCGGATGCCATACATCTCGGAAGTAGTGAAGGATTTTCAAGAGTACTCTGAGACAGGAGGGGTGAAACGTGAACGGGAGTGTGTGATGTGCCTCTCTGAGGAGATGTCCGTTGTCTTCCTCCCGTGTGCCCATCAAGTGGTTTGCAGAACATGCAATGAGCTCCACGAGAAACAGGGCATGAAGGATTGCCCGTCTTGCAGGAGCCCGATACAGTGGCGAATTTCTGTACGTTATGCTCGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

924

Amino Acids

101.76

Weight (kDa)

6.95

Isoelectric Point (pI)

52.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIR2-like_helical	PF20235	134 - 246	6.3e-31	PIR2-like, helical domain
zf-C3HC4_3	PF13920	866 - 913	5.6e-12	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008859)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g16660 FvH4_4g16660 FvH4_4g16660 FvH4_4g16660
malus_domestica	MD13G1177600.v1.1 MD16G1179600.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15320
rosa_chinensis	RchiOBHm_Chr4g0420121
rosa_laevigata	RLG00000007787
rosa_multiflora	Rmu_sc0015433.1_g000001 Rmu_sc0023895.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362830
rosa_rugosa	Rorug04G0162300 Rorug04G0162400
rosa_samantha	Rh4AG223200 Rh4BG225600 Rh4CG236400 Rh4DG222000
rosa_wichuraiana	Rw4G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	899
Acc36I	ACCTGC	2 cut(s)	899, 2266
AccB7I	CCANNNNNTGG	2 cut(s)	869, 2662
AccBSI	CCGCTC	1 cut(s)	1873
AccII	CGCG	1 cut(s)	1810
AccIII	TCCGGA	1 cut(s)	1986
AciI	CCGC	6 cut(s)	101, 447, 484, 1521, 1873, 2455
AclWI	GGATC	4 cut(s)	92, 566, 737, 2520
AcoI	YGGCCR	2 cut(s)	6, 559
AcsI	RAATTY	4 cut(s)	176, 940, 1975, 2745
AcuI	CTGAAG	3 cut(s)	477, 1518, 1898
AfaI	GTAC	8 cut(s)	620, 1438, 1462, 2043, 2268, 2375, 2570, 2754
AfiI	CCNNNNNNNGG	8 cut(s)	107, 299, 869, 1341, 1457, 1989, 2623, 2662
AflII	CTTAAG	1 cut(s)	1838
AhdI	GACNNNNNGTC	1 cut(s)	517
AjiI	CACGTC	1 cut(s)	1416
AjnI	CCWGG	5 cut(s)	868, 1368, 1959, 2214, 2508
AjuI	GAANNNNNNNTTGG	4 cut(s)	608, 640, 1588, 1620
AleI	CACNNNNGTG	1 cut(s)	2427
Alw21I	GWGCWC	1 cut(s)	2688
Alw26I	GTCTC	5 cut(s)	43, 721, 887, 2413, 2570
AlwI	GGATC	4 cut(s)	92, 566, 737, 2520
AlwNI	CAGNNNCTG	3 cut(s)	869, 1565, 2057
Ama87I	CYCGRG	1 cut(s)	1698
Aor13HI	TCCGGA	1 cut(s)	1986
AoxI	GGCC	6 cut(s)	6, 314, 559, 659, 1343, 2506
ApoI	RAATTY	4 cut(s)	176, 940, 1975, 2745
Asp700I	GAANNNNTTC	1 cut(s)	755
AspLEI	GCGC	2 cut(s)	1810, 2452
AspS9I	GGNCC	4 cut(s)	315, 1343, 1693, 2523
AsuC2I	CCSGG	5 cut(s)	92, 1342, 1699, 1700, 2527
AsuHPI	GGTGA	4 cut(s)	122, 689, 752, 2599
AsuII	TTCGAA	1 cut(s)	180
AvaI	CYCGRG	1 cut(s)	1698
AvaII	GGWCC	2 cut(s)	1693, 2523
BaeGI	GKGCMC	2 cut(s)	1744, 2656
BalI	TGGCCA	2 cut(s)	8, 561
BanII	GRGCYC	2 cut(s)	2688, 2732
BarI	GAAGNNNNNNTAC	2 cut(s)	1153, 1185
BauI	CACGAG	1 cut(s)	2690
BbsI	GAAGAC	3 cut(s)	1161, 2102, 2632
Bbv12I	GWGCWC	1 cut(s)	2688
BccI	CCATC	7 cut(s)	242, 301, 731, 761, 1924, 2229, 2664
BceAI	ACGGC	1 cut(s)	1512
BciT130I	CCWGG	5 cut(s)	870, 1370, 1961, 2216, 2510
BciVI	GTATCC	1 cut(s)	1656
BcnI	CCSGG	5 cut(s)	92, 1342, 1699, 1700, 2527
BcoDI	GTCTC	5 cut(s)	43, 721, 887, 2413, 2570
BfaI	CTAG	6 cut(s)	279, 329, 1356, 1953, 2195, 2232
BfmI	CTRYAG	5 cut(s)	602, 1506, 2049, 2186, 2366
BfrI	CTTAAG	1 cut(s)	1838
BfuAI	ACCTGC	2 cut(s)	899, 2266
BfuI	GTATCC	1 cut(s)	1656
BglII	AGATCT	2 cut(s)	598, 2209
BmcAI	AGTACT	2 cut(s)	2268, 2570
Bme18I	GGWCC	2 cut(s)	1693, 2523
BmeRI	GACNNNNNGTC	1 cut(s)	517
BmeT110I	CYCGRG	1 cut(s)	1698
BmgBI	CACGTC	1 cut(s)	1416
BmgT120I	GGNCC	4 cut(s)	315, 1343, 1693, 2523
BmiI	GGNNCC	6 cut(s)	1694, 1695, 1734, 2524, 2525, 2729
BmrI	ACTGGG	1 cut(s)	802
BmsI	GCATC	6 cut(s)	337, 670, 968, 1031, 1627, 2520
BmuI	ACTGGG	1 cut(s)	802
BpiI	GAAGAC	3 cut(s)	1161, 2102, 2632
BpmI	CTGGAG	1 cut(s)	2198
Bpu14I	TTCGAA	1 cut(s)	180
BpuEI	CTTGAG	5 cut(s)	632, 1290, 2015, 2035, 2291
BpuMI	CCSGG	5 cut(s)	92, 1342, 1699, 1700, 2527
BsaAI	YACGTR	1 cut(s)	2045
BsaBI	GATNNNNATC	1 cut(s)	741
BsaJI	CCNNGG	8 cut(s)	9, 265, 297, 1680, 1697, 1698, 1736, 2328
BsaWI	WCCGGW	2 cut(s)	1146, 1986
BsaXI	ACNNNNNCTCC	2 cut(s)	1284, 1314
Bsc4I	CCNNNNNNNGG	8 cut(s)	107, 299, 869, 1341, 1457, 1989, 2623, 2662
Bse1I	ACTGG	6 cut(s)	639, 679, 808, 863, 1613, 1902
Bse3DI	GCAATG	3 cut(s)	690, 738, 2686
Bse8I	GATNNNNATC	1 cut(s)	741
BseAI	TCCGGA	1 cut(s)	1986
BseBI	CCWGG	5 cut(s)	870, 1370, 1961, 2216, 2510
BseDI	CCNNGG	8 cut(s)	9, 265, 297, 1680, 1697, 1698, 1736, 2328
BseGI	GGATG	8 cut(s)	293, 352, 742, 1163, 1378, 1591, 2080, 2535
BseJI	GATNNNNATC	1 cut(s)	741
BseLI	CCNNNNNNNGG	8 cut(s)	107, 299, 869, 1341, 1457, 1989, 2623, 2662
BseMI	GCAATG	3 cut(s)	690, 738, 2686
BseMII	CTCAG	9 cut(s)	152, 882, 960, 987, 1178, 1563, 1917, 2565, 2613
BseNI	ACTGG	6 cut(s)	639, 679, 808, 863, 1613, 1902
BseRI	GAGGAG	1 cut(s)	2639
BseSI	GKGCMC	2 cut(s)	1744, 2656
BseYI	CCCAGC	1 cut(s)	1658
BsgI	GTGCAG	1 cut(s)	244
Bsh1236I	CGCG	1 cut(s)	1810
BshFI	GGCC	6 cut(s)	8, 316, 561, 661, 1345, 2508
BsiHKAI	GWGCWC	1 cut(s)	2688
BsiHKCI	CYCGRG	1 cut(s)	1698
BsiSI	CCGG	6 cut(s)	91, 1147, 1341, 1699, 1987, 2527
BslFI	GGGAC	5 cut(s)	779, 1679, 1715, 1760, 2509
BslI	CCNNNNNNNGG	8 cut(s)	107, 299, 869, 1341, 1457, 1989, 2623, 2662
BsmAI	GTCTC	5 cut(s)	43, 721, 887, 2413, 2570
BsmFI	GGGAC	5 cut(s)	779, 1679, 1715, 1760, 2509
BsmI	GAATGC	3 cut(s)	152, 1267, 2011
BsnI	GGCC	6 cut(s)	8, 316, 561, 661, 1345, 2508
BsoBI	CYCGRG	1 cut(s)	1698
Bsp119I	TTCGAA	1 cut(s)	180
Bsp1286I	GDGCHC	4 cut(s)	1744, 2656, 2688, 2732
Bsp13I	TCCGGA	1 cut(s)	1986
Bsp1407I	TGTACA	1 cut(s)	1436
Bsp143I	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
BspACI	CCGC	6 cut(s)	101, 447, 484, 1521, 1873, 2455
BspANI	GGCC	6 cut(s)	8, 316, 561, 661, 1345, 2508
BspCNI	CTCAG	9 cut(s)	151, 883, 961, 988, 1179, 1564, 1918, 2566, 2614
BspEI	TCCGGA	1 cut(s)	1986
BspFNI	CGCG	1 cut(s)	1810
BspLI	GGNNCC	6 cut(s)	1694, 1695, 1734, 2524, 2525, 2729
BspMAI	CTGCAG	2 cut(s)	2053, 2370
BspMI	ACCTGC	2 cut(s)	899, 2266
BspPI	GGATC	4 cut(s)	92, 566, 737, 2520
BspQI	GCTCTTC	1 cut(s)	378
BspT104I	TTCGAA	1 cut(s)	180
BspTI	CTTAAG	1 cut(s)	1838
BsrBI	CCGCTC	1 cut(s)	1873
BsrDI	GCAATG	3 cut(s)	690, 738, 2686
BsrGI	TGTACA	1 cut(s)	1436
BsrI	ACTGG	6 cut(s)	639, 679, 808, 863, 1613, 1902
BssECI	CCNNGG	8 cut(s)	9, 265, 297, 1680, 1697, 1698, 1736, 2328
BssMI	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
BssSI	CACGAG	1 cut(s)	2690
BssT1I	CCWWGG	4 cut(s)	9, 265, 1680, 2328
Bst2BI	CACGAG	1 cut(s)	2690
Bst2UI	CCWGG	5 cut(s)	870, 1370, 1961, 2216, 2510
Bst4CI	ACNGT	7 cut(s)	517, 590, 888, 1249, 1409, 1668, 2739
Bst6I	CTCTTC	2 cut(s)	46, 378
BstAFI	CTTAAG	1 cut(s)	1838
BstAPI	GCANNNNNTGC	2 cut(s)	2057, 2676
BstAUI	TGTACA	1 cut(s)	1436
BstBAI	YACGTR	1 cut(s)	2045
BstBI	TTCGAA	1 cut(s)	180
BstC8I	GCNNGC	6 cut(s)	314, 445, 1551, 1803, 2065, 2482
BstEII	GGTNACC	1 cut(s)	110
BstENI	CCTNNNNNAGG	1 cut(s)	2621
BstF5I	GGATG	8 cut(s)	293, 352, 742, 1163, 1378, 1591, 2080, 2535
BstFNI	CGCG	1 cut(s)	1810
BstHHI	GCGC	2 cut(s)	1810, 2452
BstKTI	GATC	6 cut(s)	100, 574, 601, 745, 2212, 2515
BstMAI	GTCTC	5 cut(s)	43, 721, 887, 2413, 2570
BstMBI	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
BstMWI	GCNNNNNNNGC	7 cut(s)	473, 1759, 1807, 2057, 2481, 2676, 2721
BstNI	CCWGG	5 cut(s)	870, 1370, 1961, 2216, 2510
BstNSI	RCATGY	2 cut(s)	1111, 2679
BstPI	GGTNACC	1 cut(s)	110
BstSFI	CTRYAG	5 cut(s)	602, 1506, 2049, 2186, 2366
BstSLI	GKGCMC	2 cut(s)	1744, 2656
BstUI	CGCG	1 cut(s)	1810
BstV2I	GAAGAC	3 cut(s)	1161, 2102, 2632
BstX2I	RGATCY	2 cut(s)	598, 2209
BstXI	CCANNNNNNTGG	1 cut(s)	248
BstYI	RGATCY	2 cut(s)	598, 2209
BsuI	GTATCC	1 cut(s)	1656
BsuRI	GGCC	6 cut(s)	8, 316, 561, 661, 1345, 2508
BtrI	CACGTC	1 cut(s)	1416
BtsCI	GGATG	8 cut(s)	293, 352, 742, 1163, 1378, 1591, 2080, 2535
BtsI	GCAGTG	2 cut(s)	232, 1694
BveI	ACCTGC	2 cut(s)	899, 2266
Cac8I	GCNNGC	6 cut(s)	314, 445, 1551, 1803, 2065, 2482
CaiI	CAGNNNCTG	3 cut(s)	869, 1565, 2057
CfoI	GCGC	2 cut(s)	1810, 2452
Cfr13I	GGNCC	4 cut(s)	315, 1343, 1693, 2523
Cfr9I	CCCGGG	1 cut(s)	1698
Csp6I	GTAC	8 cut(s)	619, 1437, 1461, 2042, 2267, 2374, 2569, 2753
CviQI	GTAC	8 cut(s)	619, 1437, 1461, 2042, 2267, 2374, 2569, 2753
DpnI	GATC	6 cut(s)	99, 573, 600, 744, 2211, 2514
DpnII	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
DraI	TTTAAA	1 cut(s)	1725
DriI	GACNNNNNGTC	1 cut(s)	517
EaeI	YGGCCR	2 cut(s)	6, 559
Eam1104I	CTCTTC	2 cut(s)	46, 378
Eam1105I	GACNNNNNGTC	1 cut(s)	517
EarI	CTCTTC	2 cut(s)	46, 378
EciI	GGCGGA	1 cut(s)	90
Ecl136II	GAGCTC	1 cut(s)	2686
Eco130I	CCWWGG	4 cut(s)	9, 265, 1680, 2328
Eco147I	AGGCCT	1 cut(s)	661
Eco24I	GRGCYC	2 cut(s)	2688, 2732
Eco47I	GGWCC	2 cut(s)	1693, 2523
Eco53kI	GAGCTC	1 cut(s)	2686
Eco57I	CTGAAG	3 cut(s)	477, 1518, 1898
Eco88I	CYCGRG	1 cut(s)	1698
Eco91I	GGTNACC	1 cut(s)	110
EcoICRI	GAGCTC	1 cut(s)	2686
EcoNI	CCTNNNNNAGG	1 cut(s)	2621
EcoO109I	RGGNCCY	2 cut(s)	1693, 2523
EcoO65I	GGTNACC	1 cut(s)	110
EcoRII	CCWGG	5 cut(s)	868, 1368, 1959, 2214, 2508
EcoT14I	CCWWGG	4 cut(s)	9, 265, 1680, 2328
EcoT38I	GRGCYC	2 cut(s)	2688, 2732
ErhI	CCWWGG	4 cut(s)	9, 265, 1680, 2328
FaqI	GGGAC	5 cut(s)	779, 1679, 1715, 1760, 2509
FokI	GGATG	8 cut(s)	280, 359, 749, 1170, 1385, 1578, 2087, 2542
FriOI	GRGCYC	2 cut(s)	2688, 2732
FspBI	CTAG	6 cut(s)	279, 329, 1356, 1953, 2195, 2232
GlaI	GCGC	2 cut(s)	1809, 2451
GsaI	CCCAGC	1 cut(s)	1662
GsuI	CTGGAG	1 cut(s)	2198
HaeIII	GGCC	6 cut(s)	8, 316, 561, 661, 1345, 2508
HapII	CCGG	6 cut(s)	91, 1147, 1341, 1699, 1987, 2527
HhaI	GCGC	2 cut(s)	1810, 2452
Hin6I	GCGC	2 cut(s)	1808, 2450
HinP1I	GCGC	2 cut(s)	1808, 2450
HindIII	AAGCTT	1 cut(s)	1919
HinfI	GANTC	7 cut(s)	193, 799, 841, 850, 1238, 2054, 2432
HpaII	CCGG	6 cut(s)	91, 1147, 1341, 1699, 1987, 2527
HphI	GGTGA	4 cut(s)	122, 689, 752, 2599
Hpy166II	GTNNAC	4 cut(s)	884, 990, 1773, 2597
Hpy8I	GTNNAC	4 cut(s)	884, 990, 1773, 2597
HpyCH4III	ACNGT	7 cut(s)	517, 590, 888, 1249, 1409, 1668, 2739
HpyCH4IV	ACGT	4 cut(s)	1415, 2044, 2593, 2755
HpyF10VI	GCNNNNNNNGC	7 cut(s)	473, 1759, 1807, 2057, 2481, 2676, 2721
HpySE526I	ACGT	4 cut(s)	1415, 2044, 2593, 2755
HspAI	GCGC	2 cut(s)	1808, 2450
KflI	GGGWCCC	2 cut(s)	1693, 2523
Kpn2I	TCCGGA	1 cut(s)	1986
Kzo9I	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
LguI	GCTCTTC	1 cut(s)	378
LmnI	GCTCC	4 cut(s)	770, 1767, 2691, 2727
LweI	GCATC	6 cut(s)	337, 670, 968, 1031, 1627, 2520
MaeI	CTAG	6 cut(s)	279, 329, 1356, 1953, 2195, 2232
MaeII	ACGT	4 cut(s)	1415, 2044, 2593, 2755
MalI	GATC	6 cut(s)	99, 573, 600, 744, 2211, 2514
MbiI	CCGCTC	1 cut(s)	1873
MboI	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
MfeI	CAATTG	2 cut(s)	2222, 2414
MflI	RGATCY	2 cut(s)	598, 2209
MhlI	GDGCHC	4 cut(s)	1744, 2656, 2688, 2732
MlsI	TGGCCA	2 cut(s)	8, 561
MluNI	TGGCCA	2 cut(s)	8, 561
MlyI	GAGTC	3 cut(s)	202, 1232, 2063
Mox20I	TGGCCA	2 cut(s)	8, 561
MroI	TCCGGA	1 cut(s)	1986
MroXI	GAANNNNTTC	1 cut(s)	755
MscI	TGGCCA	2 cut(s)	8, 561
MslI	CAYNNNNRTG	5 cut(s)	246, 726, 1418, 1635, 2427
Msp20I	TGGCCA	2 cut(s)	8, 561
MspA1I	CMGCKG	2 cut(s)	170, 2182
MspCI	CTTAAG	1 cut(s)	1838
MspI	CCGG	6 cut(s)	91, 1147, 1341, 1699, 1987, 2527
MunI	CAATTG	2 cut(s)	2222, 2414
Mva1269I	GAATGC	3 cut(s)	152, 1267, 2011
MvaI	CCWGG	5 cut(s)	870, 1370, 1961, 2216, 2510
MvnI	CGCG	1 cut(s)	1810
MwoI	GCNNNNNNNGC	7 cut(s)	473, 1759, 1807, 2057, 2481, 2676, 2721
NciI	CCSGG	5 cut(s)	92, 1342, 1699, 1700, 2527
NdeII	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
NlaIV	GGNNCC	6 cut(s)	1694, 1695, 1734, 2524, 2525, 2729
NmuCI	GTSAC	6 cut(s)	110, 215, 359, 1122, 1411, 1628
NspI	RCATGY	2 cut(s)	1111, 2679
NspV	TTCGAA	1 cut(s)	180
OliI	CACNNNNGTG	1 cut(s)	2427
PaqCI	CACCTGC	1 cut(s)	899
PceI	AGGCCT	1 cut(s)	661
PciSI	GCTCTTC	1 cut(s)	378
PcsI	WCGNNNNNNNCGW	1 cut(s)	2761
PctI	GAATGC	3 cut(s)	152, 1267, 2011
PdmI	GAANNNNTTC	1 cut(s)	755
PfeI	GAWTC	4 cut(s)	799, 841, 850, 2432
PflMI	CCANNNNNTGG	2 cut(s)	869, 2662
PfoI	TCCNGGA	2 cut(s)	2214, 2525
PleI	GAGTC	3 cut(s)	201, 1232, 2062
PpsI	GAGTC	3 cut(s)	201, 1232, 2062
Ppu21I	YACGTR	1 cut(s)	2045
PpuMI	RGGWCCY	2 cut(s)	1693, 2523
Psp124BI	GAGCTC	1 cut(s)	2688
Psp5II	RGGWCCY	2 cut(s)	1693, 2523
Psp6I	CCWGG	5 cut(s)	868, 1368, 1959, 2214, 2508
PspEI	GGTNACC	1 cut(s)	110
PspFI	CCCAGC	1 cut(s)	1658
PspGI	CCWGG	5 cut(s)	868, 1368, 1959, 2214, 2508
PspN4I	GGNNCC	6 cut(s)	1694, 1695, 1734, 2524, 2525, 2729
PspPI	GGNCC	4 cut(s)	315, 1343, 1693, 2523
PspPPI	RGGWCCY	2 cut(s)	1693, 2523
PstI	CTGCAG	2 cut(s)	2053, 2370
PstNI	CAGNNNCTG	3 cut(s)	869, 1565, 2057
PsuI	RGATCY	2 cut(s)	598, 2209
PvuII	CAGCTG	2 cut(s)	170, 2182
RsaI	GTAC	8 cut(s)	620, 1438, 1462, 2043, 2268, 2375, 2570, 2754
RsaNI	GTAC	8 cut(s)	619, 1437, 1461, 2042, 2267, 2374, 2569, 2753
RseI	CAYNNNNRTG	5 cut(s)	246, 726, 1418, 1635, 2427
SacI	GAGCTC	1 cut(s)	2688
SapI	GCTCTTC	1 cut(s)	378
Sau3AI	GATC	6 cut(s)	97, 571, 598, 742, 2209, 2512
Sau96I	GGNCC	4 cut(s)	315, 1343, 1693, 2523
ScaI	AGTACT	2 cut(s)	2268, 2570
SchI	GAGTC	3 cut(s)	202, 1232, 2063
SduI	GDGCHC	4 cut(s)	1744, 2656, 2688, 2732
SfaNI	GCATC	6 cut(s)	337, 670, 968, 1031, 1627, 2520
SfcI	CTRYAG	5 cut(s)	602, 1506, 2049, 2186, 2366
SfuI	TTCGAA	1 cut(s)	180
SinI	GGWCC	2 cut(s)	1693, 2523
SmaI	CCCGGG	1 cut(s)	1700
SmiMI	CAYNNNNRTG	5 cut(s)	246, 726, 1418, 1635, 2427
SmlI	CTYRAG	6 cut(s)	611, 1305, 1838, 1994, 2014, 2270
SmoI	CTYRAG	6 cut(s)	611, 1305, 1838, 1994, 2014, 2270
SseBI	AGGCCT	1 cut(s)	661
SsiI	CCGC	6 cut(s)	101, 447, 484, 1521, 1873, 2455
SspMI	CTAG	6 cut(s)	279, 329, 1356, 1953, 2195, 2232
SstI	GAGCTC	1 cut(s)	2688
StuI	AGGCCT	1 cut(s)	661
StyI	CCWWGG	4 cut(s)	9, 265, 1680, 2328
TaaI	ACNGT	7 cut(s)	517, 590, 888, 1249, 1409, 1668, 2739
TaiI	ACGT	4 cut(s)	1418, 2047, 2596, 2758
TaqI	TCGA	7 cut(s)	180, 261, 578, 1072, 2285, 2397, 2499
TatI	WGTACW	5 cut(s)	618, 1436, 2266, 2373, 2568
TauI	GCSGC	2 cut(s)	1523, 1876
TfiI	GAWTC	4 cut(s)	799, 841, 850, 2432
TseFI	GTSAC	6 cut(s)	110, 215, 359, 1122, 1411, 1628
Tsp45I	GTSAC	6 cut(s)	110, 215, 359, 1122, 1411, 1628
TspDTI	ATGAA	8 cut(s)	725, 748, 941, 1070, 1728, 1928, 2120, 2720
TspGWI	ACGGA	3 cut(s)	781, 1972, 2623
TspMI	CCCGGG	1 cut(s)	1698
Van91I	CCANNNNNTGG	2 cut(s)	869, 2662
Vha464I	CTTAAG	1 cut(s)	1838
VpaK11BI	GGWCC	2 cut(s)	1693, 2523
XagI	CCTNNNNNAGG	1 cut(s)	2621
XapI	RAATTY	4 cut(s)	176, 940, 1975, 2745
XceI	RCATGY	2 cut(s)	1111, 2679
XmaI	CCCGGG	1 cut(s)	1698
XmnI	GAANNNNTTC	1 cut(s)	755
XspI	CTAG	6 cut(s)	279, 329, 1356, 1953, 2195, 2232
ZrmI	AGTACT	2 cut(s)	2268, 2570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.