Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008859

Physical Location & Seq

Genomic Coordinates Reverse (-)

15402174 .. 15406483

Gene Structure 4310 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1179600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2817 bp

ATGAATGTTGGTTTCAGTACTGAAATGGCATCAATGGTGGCCAAGGGCAGTAGTAGTTGTACCACCCAAGTCTCTTCCTCGATAACTGTTCAAGAAAAGGGAAGTAGAAATAAGAGGAAGTTCCGGACTGATCCGCCTTTAGGTTACCCGAGCAAGATTATTCCTTTATCTCAGACTGAATCAACAAGTTATGAGTTTTCAGCTGAAAAATTCGAAATCACGCAGAGTCATGGACAAATTGGTGTCTGTGACCTGTGCAGTATTAATCAAGACCATTCTGATGGGTTGAAACTCGACCTTGGATTGTCTAATGCTGTTGTTCCATCCGAGGTTGGCCCAAGCCGGCCCAGAGAGGAACTAGAGGCAGATGAGTTTCAGGATGCTGATTGGAGTGACCTCACAGAAACCCAACTTGAAGAGCTTGTTCTAAGTAATTTGGACATGATTTTCAACAGTGCAATAAAAAAGATTGTTGCTTGTGGTTACGCAGAAGAAGTTGCTACAAAGGCTGTTTTGCGGTCTGGTCTTTGTTATGGGTGTAAAGACACCGTGTCTAATATAGTGGATAATACTTTAGCTTATCTTAGAAGTGGCCAAGAAATTGATCCTTCAAGAGAGCACTGTTTTGAAGATCTACAGCAGCTGGAGAAGTACATATTGGCAGAACTGGTTTGCGTTCTTCGAGAGGTTAGGCCTTTCTTCAGCACTGGTGATGCAATGTGGTGCTTGTTGATTTGTGACATGAATGTCTCTCATGCTTGTGCAATGGATGGCGATCCCTTGAATAGTTTCATTGGTGATGGAGCTTCAAATGGAAGTCCCTCCATCCCGAATCAACCCCAGTCAAAAACAGAATCCAAAAGTTCGGAATTGAATATTCTGAATAATTCTAAGACAGTTCCTTCAGTTCCTGGTTCTCATAGCTCACAGTCTGAGACACCTACCATTGCAGGAGGGGTTCCCAGCATAGCTAAACCGAAAAATTCCGTTGTTCATAGTGGATCATTGTTTGAGAAAGAGGGCGCACATTCCACATCTGATAATGGGGATAAATCTTTTTGTGCATCTGAAACATCTCAATCTCCCACAGTAGAGGAAAAGCTTTTAAGTAGTAGAAGGGTCCATTCTGTTACTACCAAGAGAGACTACATGCTTCGGCACAAGTCGCTTCATTTGGAGAAAAGCTATCGGACTTATGGATGTAAAGGGTCTTCAAGGGCTGGGAAGCTGAATGGTTTGGGTGGTTTAATCTTGGATAAGAAACTAAAATCTGTGTCAGACTCTACCGCTGTTAATTTAAAGAATGCTTCCTTAAAAATAAGCAAGGCTATGGGAGTTGATGTGCCTCAAGACAGTGGAAACAACAATCTTTCTGCCAATGCAGGGCCTTCTTCCCCTAGACCATTTAACCTGGATGTTGACAATACTGCTTCTGTTTTGCCTAAGAACAGTGTCCCGTCCATATTACCTGCTGTCGGTACTTCAACGGCATTACCTGCTGTTGGTACTTCAACAGCATTACCTTCTGTCAGTACTTCAATGGCATTACCTGTAGTCAATACTGCAGCAGCATTACCTGCACCAAATACTCTGCCTGCATTATCAGTTGCTGATACTGAGCTTTCCCTTTCTCTGCCTACAAAAAGCATTTCAAATCCAGTTCCTATTAGCTGTCACTCTGATGCAACTAATTCTGTTTTTTCTGGGATACCGTATGATAAGTCCTTGGGGCAGTGGGTTCCCCGGGACAAGAAAGATGAAATGATTTTGAAGTTGGTTCCGAGGGCACGGGACTTGCAAAATCAGCTCCAAGAGTGGACAGAGTGGGCCAATCAGAAGGTTATGCAGGCTGCACGTAGATTGAGTAAGGACAAGGCTGAACTTAAGTCATTGAGGCAAGAGAAAGAAGAAGTTGAGCGGCTGAAGAAAGAGAAGCAAACGCTGGAGGAAAACACCATGAAGAAGCTTTCTGAGATGGAAAATTCTCTGTGCAAGGCTAGTAGCCAGGTTGAACGAGCAAATTCTTCTGTCCGGAGGCTTGAGGTGGAGAATGCTGCCTTGAGGCAGGAAATGGAGGCTGCTAAAGTACGTGCTGCAGAGTCCGCTGCAAGCTGTCAGGAGGTATCAAAGAGGGAGAAGAAAACACTGATAAAGTTTCAGTCCTGGGAGAAGCAGAAAACCATGTTCAATGGAGAACTTGTGACTGAAAAGCGCAAGTTAAAACAGCTGCTACAGGAACTAGAACAAGCGAAAGATCTCCAGGAACAATTGGAGGCTAGATGGCAACAGGAAGAGAAATCAAAGGAAGAACTATTTGGGCAGGTCAGTTCTATAAGAAAAGAAAGGGAACAGATAGAGGCTTCAACAAAATCCGAGGAGGATGCGATTAAACTGAAGGCAGAAAACAATCTGCAGAAGTACAAAGACGATATTCAGAAACTCGAAAAAGAAATCTCTCAATTGAGACTCAAGAGTGATTCTTCAAAAATTGCTGCGCTGCGGAGGGGCGTTGATGGAAGCTATTCCAGCAAAGTAATAGACATCGAAAATGGCCTGGATGACAAGGGGTCCCGGATCCCATACATCTCAGAAGCAGTGAAGGATATTCAAGACTACACTGAGACAGGAGGGGTGAAACGTGAACGGGAGTGTGTCATGTGCCTCTCGGAGGAGATGTCTGTGGTCTTCCTCCCGTGCGCCCATCAAGTGGTATGCAGGACGTGCAATGAGCTCCACGAGAAACAGGGCATGAAGGATTGCCCGTCTTGTAGGAGCCCGATACAGTGGCGAATTTCTGTACGTTATGCTCGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

939

Amino Acids

103.2

Weight (kDa)

6.24

Isoelectric Point (pI)

52.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIR2-like_helical	PF20235	145 - 257	2.2e-30	PIR2-like, helical domain
zf-C3HC4_3	PF13920	881 - 928	5.7e-12	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008859)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g16660 FvH4_4g16660 FvH4_4g16660 FvH4_4g16660
malus_domestica	MD13G1177600.v1.1 MD16G1179600.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15320
rosa_chinensis	RchiOBHm_Chr4g0420121
rosa_laevigata	RLG00000007787
rosa_multiflora	Rmu_sc0015433.1_g000001 Rmu_sc0023895.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362830
rosa_rugosa	Rorug04G0162300 Rorug04G0162400
rosa_samantha	Rh4AG223200 Rh4BG225600 Rh4CG236400 Rh4DG222000
rosa_wichuraiana	Rw4G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	746
Acc36I	ACCTGC	4 cut(s)	1477, 1504, 1585, 2311
AccB7I	CCANNNNNTGG	1 cut(s)	2707
AccBSI	CCGCTC	1 cut(s)	1918
AccIII	TCCGGA	2 cut(s)	123, 2031
AciI	CCGC	6 cut(s)	134, 517, 1287, 1918, 2103, 2500
AclWI	GGATC	6 cut(s)	125, 599, 770, 1009, 2569, 2582
AcoI	YGGCCR	2 cut(s)	39, 592
AcsI	RAATTY	5 cut(s)	209, 982, 1981, 2020, 2790
AcuI	CTGAAG	4 cut(s)	685, 888, 1943, 2414
AfaI	GTAC	9 cut(s)	19, 61, 653, 1480, 1507, 1534, 2088, 2420, 2799
AfiI	CCNNNNNNNGG	6 cut(s)	140, 1383, 1475, 1502, 2668, 2707
AflII	CTTAAG	1 cut(s)	1883
AhdI	GACNNNNNGTC	2 cut(s)	550, 2566
AjiI	CACGTC	1 cut(s)	2721
AjnI	CCWGG	6 cut(s)	910, 1410, 2004, 2162, 2259, 2553
AjuI	GAANNNNNNNTTGG	4 cut(s)	641, 673, 2298, 2330
Alw21I	GWGCWC	2 cut(s)	621, 2733
Alw26I	GTCTC	6 cut(s)	76, 754, 929, 1137, 2458, 2615
AlwI	GGATC	6 cut(s)	125, 599, 770, 1009, 2569, 2582
AlwNI	CAGNNNCTG	3 cut(s)	643, 911, 1610
Ama87I	CYCGRG	2 cut(s)	148, 1743
Aor13HI	TCCGGA	2 cut(s)	123, 2031
AoxI	GGCC	8 cut(s)	39, 334, 344, 592, 692, 1385, 1827, 2551
ApoI	RAATTY	5 cut(s)	209, 982, 1981, 2020, 2790
AseI	ATTAAT	1 cut(s)	264
Asp700I	GAANNNNTTC	2 cut(s)	788, 2521
AspLEI	GCGC	4 cut(s)	1025, 2214, 2497, 2699
AspS9I	GGNCC	6 cut(s)	335, 345, 1120, 1385, 1827, 2568
AsuC2I	CCSGG	3 cut(s)	1744, 1745, 2572
AsuHPI	GGTGA	3 cut(s)	722, 809, 2644
AsuII	TTCGAA	1 cut(s)	213
AvaI	CYCGRG	2 cut(s)	148, 1743
AvaII	GGWCC	2 cut(s)	1120, 2568
BaeGI	GKGCMC	1 cut(s)	1789
BalI	TGGCCA	2 cut(s)	41, 594
BamHI	GGATCC	1 cut(s)	2574
BanII	GRGCYC	2 cut(s)	2733, 2777
BarI	GAAGNNNNNNTAC	2 cut(s)	1195, 1227
BauI	CACGAG	1 cut(s)	2735
BbsI	GAAGAC	2 cut(s)	1203, 2677
Bbv12I	GWGCWC	2 cut(s)	621, 2733
BccI	CCATC	9 cut(s)	275, 331, 764, 794, 833, 1969, 2274, 2507, 2709
BceAI	ACGGC	1 cut(s)	1503
BcgI	CGANNNNNNTGC	2 cut(s)	2363, 2397
BciT130I	CCWGG	6 cut(s)	912, 1412, 2006, 2164, 2261, 2555
BciVI	GTATCC	1 cut(s)	1701
BcnI	CCSGG	3 cut(s)	1744, 1745, 2572
BcoDI	GTCTC	6 cut(s)	76, 754, 929, 1137, 2458, 2615
BfaI	CTAG	5 cut(s)	359, 1398, 1998, 2240, 2277
BfmI	CTRYAG	6 cut(s)	635, 1551, 1563, 2094, 2231, 2411
BfrI	CTTAAG	1 cut(s)	1883
BfuAI	ACCTGC	4 cut(s)	1477, 1504, 1585, 2311
BfuI	GTATCC	1 cut(s)	1701
BglII	AGATCT	2 cut(s)	631, 2254
BmcAI	AGTACT	2 cut(s)	19, 1534
Bme1390I	CCNGG	9 cut(s)	912, 1412, 1744, 1745, 2006, 2164, 2261, 2555, 2572
Bme18I	GGWCC	2 cut(s)	1120, 2568
BmeRI	GACNNNNNGTC	2 cut(s)	550, 2566
BmeT110I	CYCGRG	2 cut(s)	148, 1743
BmgBI	CACGTC	1 cut(s)	2721
BmgT120I	GGNCC	6 cut(s)	335, 345, 1120, 1385, 1827, 2568
BmiI	GGNNCC	8 cut(s)	960, 1121, 1740, 1779, 2569, 2570, 2576, 2774
BmrFI	CCNGG	9 cut(s)	912, 1412, 1744, 1745, 2006, 2164, 2261, 2555, 2572
BmrI	ACTGGG	1 cut(s)	835
BmsI	GCATC	6 cut(s)	38, 370, 703, 1073, 1672, 2371
BmuI	ACTGGG	1 cut(s)	835
BpiI	GAAGAC	2 cut(s)	1203, 2677
BpmI	CTGGAG	3 cut(s)	665, 1964, 2243
Bpu14I	TTCGAA	1 cut(s)	213
BpuEI	CTTGAG	4 cut(s)	1332, 2060, 2080, 2453
BpuMI	CCSGG	3 cut(s)	1744, 1745, 2572
BsaAI	YACGTR	2 cut(s)	1856, 2090
BsaBI	GATNNNNATC	1 cut(s)	774
BsaJI	CCNNGG	9 cut(s)	42, 298, 327, 1725, 1742, 1743, 1781, 2163, 2373
BsaWI	WCCGGW	2 cut(s)	123, 2031
BsaXI	ACNNNNNCTCC	6 cut(s)	1326, 1356, 2184, 2214, 2660, 2690
Bsc4I	CCNNNNNNNGG	6 cut(s)	140, 1383, 1475, 1502, 2668, 2707
Bse118I	RCCGGY	1 cut(s)	342
Bse1I	ACTGG	4 cut(s)	672, 712, 841, 1658
Bse3DI	GCAATG	4 cut(s)	723, 771, 945, 2731
Bse8I	GATNNNNATC	1 cut(s)	774
BseAI	TCCGGA	2 cut(s)	123, 2031
BseBI	CCWGG	6 cut(s)	912, 1412, 2006, 2164, 2261, 2555
BseDI	CCNNGG	9 cut(s)	42, 298, 327, 1725, 1742, 1743, 1781, 2163, 2373
BseGI	GGATG	8 cut(s)	323, 385, 775, 825, 1205, 1420, 2386, 2563
BseJI	GATNNNNATC	1 cut(s)	774
BseLI	CCNNNNNNNGG	6 cut(s)	140, 1383, 1475, 1502, 2668, 2707
BseMI	GCAATG	4 cut(s)	723, 771, 945, 2731
BseMII	CTCAG	6 cut(s)	185, 924, 1608, 1962, 2601, 2610
BseNI	ACTGG	4 cut(s)	672, 712, 841, 1658
BseRI	GAGGAG	2 cut(s)	2390, 2684
BseSI	GKGCMC	1 cut(s)	1789
BseYI	CCCAGC	2 cut(s)	962, 1220
BsgI	GTGCAG	3 cut(s)	277, 1563, 1836
BshFI	GGCC	8 cut(s)	41, 336, 346, 594, 694, 1387, 1829, 2553
BsiHKAI	GWGCWC	2 cut(s)	621, 2733
BsiHKCI	CYCGRG	2 cut(s)	148, 1743
BsiSI	CCGG	5 cut(s)	124, 343, 1744, 2032, 2572
BslFI	GGGAC	5 cut(s)	804, 1439, 1760, 1805, 2554
BslI	CCNNNNNNNGG	6 cut(s)	140, 1383, 1475, 1502, 2668, 2707
BsmAI	GTCTC	6 cut(s)	76, 754, 929, 1137, 2458, 2615
BsmFI	GGGAC	5 cut(s)	804, 1439, 1760, 1805, 2554
BsmI	GAATGC	2 cut(s)	1309, 2056
BsnI	GGCC	8 cut(s)	41, 336, 346, 594, 694, 1387, 1829, 2553
BsoBI	CYCGRG	2 cut(s)	148, 1743
Bsp119I	TTCGAA	1 cut(s)	213
Bsp1286I	GDGCHC	4 cut(s)	621, 1789, 2733, 2777
Bsp13I	TCCGGA	2 cut(s)	123, 2031
Bsp143I	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
BspACI	CCGC	6 cut(s)	134, 517, 1287, 1918, 2103, 2500
BspANI	GGCC	8 cut(s)	41, 336, 346, 594, 694, 1387, 1829, 2553
BspCNI	CTCAG	6 cut(s)	184, 925, 1609, 1963, 2600, 2611
BspEI	TCCGGA	2 cut(s)	123, 2031
BspLI	GGNNCC	8 cut(s)	960, 1121, 1740, 1779, 2569, 2570, 2576, 2774
BspMAI	CTGCAG	3 cut(s)	1567, 2098, 2415
BspMI	ACCTGC	4 cut(s)	1477, 1504, 1585, 2311
BspPI	GGATC	6 cut(s)	125, 599, 770, 1009, 2569, 2582
BspQI	GCTCTTC	1 cut(s)	411
BspT104I	TTCGAA	1 cut(s)	213
BspTI	CTTAAG	1 cut(s)	1883
BsrBI	CCGCTC	1 cut(s)	1918
BsrDI	GCAATG	4 cut(s)	723, 771, 945, 2731
BsrFI	RCCGGY	1 cut(s)	342
BsrI	ACTGG	4 cut(s)	672, 712, 841, 1658
BssAI	RCCGGY	1 cut(s)	342
BssECI	CCNNGG	9 cut(s)	42, 298, 327, 1725, 1742, 1743, 1781, 2163, 2373
BssMI	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
BssSI	CACGAG	1 cut(s)	2735
BssT1I	CCWWGG	3 cut(s)	42, 298, 1725
Bst2BI	CACGAG	1 cut(s)	2735
Bst2UI	CCWGG	6 cut(s)	912, 1412, 2006, 2164, 2261, 2555
Bst6I	CTCTTC	3 cut(s)	79, 411, 2286
BstAFI	CTTAAG	1 cut(s)	1883
BstAPI	GCANNNNNTGC	3 cut(s)	1496, 1577, 2721
BstBAI	YACGTR	2 cut(s)	1856, 2090
BstBI	TTCGAA	1 cut(s)	213
BstC8I	GCNNGC	4 cut(s)	344, 1596, 1848, 2110
BstEII	GGTNACC	1 cut(s)	143
BstENI	CCTNNNNNAGG	1 cut(s)	2666
BstF5I	GGATG	8 cut(s)	323, 385, 775, 825, 1205, 1420, 2386, 2563
BstHHI	GCGC	4 cut(s)	1025, 2214, 2497, 2699
BstKTI	GATC	7 cut(s)	133, 607, 634, 778, 1004, 2257, 2577
BstMAI	GTCTC	6 cut(s)	76, 754, 929, 1137, 2458, 2615
BstMBI	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
BstMWI	GCNNNNNNNGC	8 cut(s)	506, 1165, 1496, 1577, 1804, 2102, 2526, 2721
BstNI	CCWGG	6 cut(s)	912, 1412, 2006, 2164, 2261, 2555
BstNSI	RCATGY	1 cut(s)	1153
BstPI	GGTNACC	1 cut(s)	143
BstSCI	CCNGG	9 cut(s)	910, 1410, 1742, 1743, 2004, 2162, 2259, 2553, 2570
BstSFI	CTRYAG	6 cut(s)	635, 1551, 1563, 2094, 2231, 2411
BstSLI	GKGCMC	1 cut(s)	1789
BstV2I	GAAGAC	2 cut(s)	1203, 2677
BstX2I	RGATCY	3 cut(s)	631, 2254, 2574
BstXI	CCANNNNNNTGG	1 cut(s)	281
BstYI	RGATCY	3 cut(s)	631, 2254, 2574
BsuI	GTATCC	1 cut(s)	1701
BsuRI	GGCC	8 cut(s)	41, 336, 346, 594, 694, 1387, 1829, 2553
BtrI	CACGTC	1 cut(s)	2721
BtsCI	GGATG	8 cut(s)	323, 385, 775, 825, 1205, 1420, 2386, 2563
BtsI	GCAGTG	2 cut(s)	1739, 2601
BveI	ACCTGC	4 cut(s)	1477, 1504, 1585, 2311
Cac8I	GCNNGC	4 cut(s)	344, 1596, 1848, 2110
CaiI	CAGNNNCTG	3 cut(s)	643, 911, 1610
CfoI	GCGC	4 cut(s)	1025, 2214, 2497, 2699
Cfr10I	RCCGGY	1 cut(s)	342
Cfr13I	GGNCC	6 cut(s)	335, 345, 1120, 1385, 1827, 2568
Cfr9I	CCCGGG	1 cut(s)	1743
Csp6I	GTAC	9 cut(s)	18, 60, 652, 1479, 1506, 1533, 2087, 2419, 2798
CviAII	CATG	9 cut(s)	230, 442, 742, 755, 1150, 1957, 2182, 2656, 2749
CviQI	GTAC	9 cut(s)	18, 60, 652, 1479, 1506, 1533, 2087, 2419, 2798
DpnI	GATC	7 cut(s)	132, 606, 633, 777, 1003, 2256, 2576
DpnII	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
DraI	TTTAAA	1 cut(s)	1299
DrdI	GACNNNNNNGTC	1 cut(s)	746
DriI	GACNNNNNGTC	2 cut(s)	550, 2566
DseDI	GACNNNNNNGTC	1 cut(s)	746
EaeI	YGGCCR	2 cut(s)	39, 592
Eam1104I	CTCTTC	3 cut(s)	79, 411, 2286
Eam1105I	GACNNNNNGTC	2 cut(s)	550, 2566
EarI	CTCTTC	3 cut(s)	79, 411, 2286
EciI	GGCGGA	1 cut(s)	123
Ecl136II	GAGCTC	1 cut(s)	2731
Eco130I	CCWWGG	3 cut(s)	42, 298, 1725
Eco147I	AGGCCT	1 cut(s)	694
Eco24I	GRGCYC	2 cut(s)	2733, 2777
Eco47I	GGWCC	2 cut(s)	1120, 2568
Eco53kI	GAGCTC	1 cut(s)	2731
Eco57I	CTGAAG	4 cut(s)	685, 888, 1943, 2414
Eco88I	CYCGRG	2 cut(s)	148, 1743
Eco91I	GGTNACC	1 cut(s)	143
EcoICRI	GAGCTC	1 cut(s)	2731
EcoNI	CCTNNNNNAGG	1 cut(s)	2666
EcoO109I	RGGNCCY	2 cut(s)	1385, 2568
EcoO65I	GGTNACC	1 cut(s)	143
EcoRII	CCWGG	6 cut(s)	910, 1410, 2004, 2162, 2259, 2553
EcoT14I	CCWWGG	3 cut(s)	42, 298, 1725
EcoT38I	GRGCYC	2 cut(s)	2733, 2777
ErhI	CCWWGG	3 cut(s)	42, 298, 1725
FaeI	CATG	9 cut(s)	233, 445, 745, 758, 1153, 1960, 2185, 2659, 2752
FaqI	GGGAC	5 cut(s)	804, 1439, 1760, 1805, 2554
FatI	CATG	9 cut(s)	229, 441, 741, 754, 1149, 1956, 2181, 2655, 2748
FokI	GGATG	8 cut(s)	310, 392, 782, 812, 1212, 1427, 2393, 2570
FriOI	GRGCYC	2 cut(s)	2733, 2777
FspBI	CTAG	5 cut(s)	359, 1398, 1998, 2240, 2277
GlaI	GCGC	4 cut(s)	1024, 2213, 2496, 2698
GsaI	CCCAGC	2 cut(s)	966, 1224
GsuI	CTGGAG	3 cut(s)	665, 1964, 2243
HaeIII	GGCC	8 cut(s)	41, 336, 346, 594, 694, 1387, 1829, 2553
HapII	CCGG	5 cut(s)	124, 343, 1744, 2032, 2572
HhaI	GCGC	4 cut(s)	1025, 2214, 2497, 2699
Hin1II	CATG	9 cut(s)	233, 445, 745, 758, 1153, 1960, 2185, 2659, 2752
Hin6I	GCGC	4 cut(s)	1023, 2212, 2495, 2697
HinP1I	GCGC	4 cut(s)	1023, 2212, 2495, 2697
HincII	GTYRAC	1 cut(s)	1420
HindII	GTYRAC	1 cut(s)	1420
HindIII	AAGCTT	2 cut(s)	1100, 1964
HinfI	GANTC	8 cut(s)	179, 226, 832, 854, 1280, 2099, 2466, 2477
HpaII	CCGG	5 cut(s)	124, 343, 1744, 2032, 2572
HphI	GGTGA	3 cut(s)	722, 809, 2644
Hpy166II	GTNNAC	3 cut(s)	1420, 1818, 2642
Hpy8I	GTNNAC	3 cut(s)	1420, 1818, 2642
HpyAV	CCTTC	9 cut(s)	618, 912, 1110, 1398, 1533, 1831, 2389, 2593, 2746
HpyCH4IV	ACGT	5 cut(s)	1855, 2089, 2638, 2720, 2800
HpyF10VI	GCNNNNNNNGC	8 cut(s)	506, 1165, 1496, 1577, 1804, 2102, 2526, 2721
HpySE526I	ACGT	5 cut(s)	1855, 2089, 2638, 2720, 2800
Hsp92II	CATG	9 cut(s)	233, 445, 745, 758, 1153, 1960, 2185, 2659, 2752
HspAI	GCGC	4 cut(s)	1023, 2212, 2495, 2697
KflI	GGGWCCC	1 cut(s)	2568
Kpn2I	TCCGGA	2 cut(s)	123, 2031
KroI	GCCGGC	1 cut(s)	342
KroNI	GCCGGC	1 cut(s)	344
Kzo9I	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
LguI	GCTCTTC	1 cut(s)	411
LmnI	GCTCC	4 cut(s)	803, 1812, 2736, 2772
LweI	GCATC	6 cut(s)	38, 370, 703, 1073, 1672, 2371
MaeI	CTAG	5 cut(s)	359, 1398, 1998, 2240, 2277
MaeII	ACGT	5 cut(s)	1855, 2089, 2638, 2720, 2800
MaeIII	GTNAC	8 cut(s)	143, 248, 392, 482, 737, 1129, 1673, 2200
MalI	GATC	7 cut(s)	132, 606, 633, 777, 1003, 2256, 2576
MbiI	CCGCTC	1 cut(s)	1918
MboI	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
MfeI	CAATTG	2 cut(s)	2267, 2459
MflI	RGATCY	3 cut(s)	631, 2254, 2574
MhlI	GDGCHC	4 cut(s)	621, 1789, 2733, 2777
MlsI	TGGCCA	2 cut(s)	41, 594
MluNI	TGGCCA	2 cut(s)	41, 594
MlyI	GAGTC	4 cut(s)	235, 1274, 2108, 2460
Mox20I	TGGCCA	2 cut(s)	41, 594
MroI	TCCGGA	2 cut(s)	123, 2031
MroNI	GCCGGC	1 cut(s)	342
MroXI	GAANNNNTTC	2 cut(s)	788, 2521
MscI	TGGCCA	2 cut(s)	41, 594
MslI	CAYNNNNRTG	3 cut(s)	279, 759, 1680
Msp20I	TGGCCA	2 cut(s)	41, 594
MspA1I	CMGCKG	5 cut(s)	203, 643, 1289, 2105, 2227
MspCI	CTTAAG	1 cut(s)	1883
MspI	CCGG	5 cut(s)	124, 343, 1744, 2032, 2572
MspR9I	CCNGG	9 cut(s)	912, 1412, 1744, 1745, 2006, 2164, 2261, 2555, 2572
MunI	CAATTG	2 cut(s)	2267, 2459
Mva1269I	GAATGC	2 cut(s)	1309, 2056
MvaI	CCWGG	6 cut(s)	912, 1412, 2006, 2164, 2261, 2555
MwoI	GCNNNNNNNGC	8 cut(s)	506, 1165, 1496, 1577, 1804, 2102, 2526, 2721
NaeI	GCCGGC	1 cut(s)	344
NciI	CCSGG	3 cut(s)	1744, 1745, 2572
NdeII	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
NgoMIV	GCCGGC	1 cut(s)	342
NlaIII	CATG	9 cut(s)	233, 445, 745, 758, 1153, 1960, 2185, 2659, 2752
NlaIV	GGNNCC	8 cut(s)	960, 1121, 1740, 1779, 2569, 2570, 2576, 2774
NmuCI	GTSAC	5 cut(s)	248, 392, 737, 1673, 2200
NspI	RCATGY	1 cut(s)	1153
NspV	TTCGAA	1 cut(s)	213
PceI	AGGCCT	1 cut(s)	694
PciSI	GCTCTTC	1 cut(s)	411
PcsI	WCGNNNNNNNCGW	1 cut(s)	2806
PctI	GAATGC	2 cut(s)	1309, 2056
PdiI	GCCGGC	1 cut(s)	344
PdmI	GAANNNNTTC	2 cut(s)	788, 2521
PfeI	GAWTC	4 cut(s)	179, 832, 854, 2477
PflMI	CCANNNNNTGG	1 cut(s)	2707
PfoI	TCCNGGA	2 cut(s)	2259, 2570
PleI	GAGTC	4 cut(s)	234, 1274, 2107, 2460
PpsI	GAGTC	4 cut(s)	234, 1274, 2107, 2460
Ppu21I	YACGTR	2 cut(s)	1856, 2090
PpuMI	RGGWCCY	1 cut(s)	2568
PshBI	ATTAAT	1 cut(s)	264
Psp124BI	GAGCTC	1 cut(s)	2733
Psp5II	RGGWCCY	1 cut(s)	2568
Psp6I	CCWGG	6 cut(s)	910, 1410, 2004, 2162, 2259, 2553
PspEI	GGTNACC	1 cut(s)	143
PspFI	CCCAGC	2 cut(s)	962, 1220
PspGI	CCWGG	6 cut(s)	910, 1410, 2004, 2162, 2259, 2553
PspN4I	GGNNCC	8 cut(s)	960, 1121, 1740, 1779, 2569, 2570, 2576, 2774
PspPI	GGNCC	6 cut(s)	335, 345, 1120, 1385, 1827, 2568
PspPPI	RGGWCCY	1 cut(s)	2568
PstI	CTGCAG	3 cut(s)	1567, 2098, 2415
PstNI	CAGNNNCTG	3 cut(s)	643, 911, 1610
PsuI	RGATCY	3 cut(s)	631, 2254, 2574
PvuII	CAGCTG	3 cut(s)	203, 643, 2227
RsaI	GTAC	9 cut(s)	19, 61, 653, 1480, 1507, 1534, 2088, 2420, 2799
RsaNI	GTAC	9 cut(s)	18, 60, 652, 1479, 1506, 1533, 2087, 2419, 2798
RseI	CAYNNNNRTG	3 cut(s)	279, 759, 1680
SacI	GAGCTC	1 cut(s)	2733
SapI	GCTCTTC	1 cut(s)	411
Sau3AI	GATC	7 cut(s)	130, 604, 631, 775, 1001, 2254, 2574
Sau96I	GGNCC	6 cut(s)	335, 345, 1120, 1385, 1827, 2568
ScaI	AGTACT	2 cut(s)	19, 1534
SchI	GAGTC	4 cut(s)	235, 1274, 2108, 2460
ScrFI	CCNGG	9 cut(s)	912, 1412, 1744, 1745, 2006, 2164, 2261, 2555, 2572
SduI	GDGCHC	4 cut(s)	621, 1789, 2733, 2777
SfaNI	GCATC	6 cut(s)	38, 370, 703, 1073, 1672, 2371
SfcI	CTRYAG	6 cut(s)	635, 1551, 1563, 2094, 2231, 2411
SfuI	TTCGAA	1 cut(s)	213
SinI	GGWCC	2 cut(s)	1120, 2568
SmaI	CCCGGG	1 cut(s)	1745
SmiMI	CAYNNNNRTG	3 cut(s)	279, 759, 1680
SmlI	CTYRAG	5 cut(s)	1347, 1883, 2039, 2059, 2468
SmoI	CTYRAG	5 cut(s)	1347, 1883, 2039, 2059, 2468
SseBI	AGGCCT	1 cut(s)	694
SsiI	CCGC	6 cut(s)	134, 517, 1287, 1918, 2103, 2500
SspI	AATATT	1 cut(s)	877
SspMI	CTAG	5 cut(s)	359, 1398, 1998, 2240, 2277
SstI	GAGCTC	1 cut(s)	2733
StuI	AGGCCT	1 cut(s)	694
StyD4I	CCNGG	9 cut(s)	910, 1410, 1742, 1743, 2004, 2162, 2259, 2553, 2570
StyI	CCWWGG	3 cut(s)	42, 298, 1725
TaiI	ACGT	5 cut(s)	1858, 2092, 2641, 2723, 2803
TaqI	TCGA	6 cut(s)	80, 213, 294, 682, 2442, 2544
TatI	WGTACW	4 cut(s)	17, 651, 1532, 2418
TauI	GCSGC	1 cut(s)	1921
TfiI	GAWTC	4 cut(s)	179, 832, 854, 2477
TseFI	GTSAC	5 cut(s)	248, 392, 737, 1673, 2200
Tsp45I	GTSAC	5 cut(s)	248, 392, 737, 1673, 2200
TspDTI	ATGAA	8 cut(s)	17, 758, 781, 983, 1160, 1773, 1973, 2765
TspGWI	ACGGA	1 cut(s)	976
TspMI	CCCGGG	1 cut(s)	1743
Van91I	CCANNNNNTGG	1 cut(s)	2707
Vha464I	CTTAAG	1 cut(s)	1883
VpaK11BI	GGWCC	2 cut(s)	1120, 2568
VspI	ATTAAT	1 cut(s)	264
XagI	CCTNNNNNAGG	1 cut(s)	2666
XapI	RAATTY	5 cut(s)	209, 982, 1981, 2020, 2790
XceI	RCATGY	1 cut(s)	1153
XmaI	CCCGGG	1 cut(s)	1743
XmnI	GAANNNNTTC	2 cut(s)	788, 2521
XspI	CTAG	5 cut(s)	359, 1398, 1998, 2240, 2277
ZrmI	AGTACT	2 cut(s)	19, 1534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.