Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0008859

Physical Location & Seq

Genomic Coordinates Forward (+)

43902373 .. 43906776

Gene Structure 4404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00362830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2667 bp

ATGGCGTCAATGGTTGCCAAGGGGAGTAGTTGTACCACTCAAGTGTCTCCCTCTATAACTGTTCAAGAAAAGGGTAGTAGAAATAAGAGGAAGTTCCGGGCTGATCCACCTTTAGGGGACCAGAATAAGATTTTGCCTTTACCTCAAACTGAATGTGCAAGTTACGAATTTTCAGCGGAAAAATTTGAAATCAGTCACCAGAATCATGGACAAACCAGTGTCTGTGACCTGTGTTTTGTTAATCAAGACCATTCTGATGGGTTGAAACTTGACCTTGGATTGTCTAGTGCTGTTGGTTCATCTGAGGTTGGCCCAAGCCGGCCTAGAAGGGAATCAGAGGCAGAAGAGTTTCAAGATGCTGATTGGAGTGATCTCACGGAAACCCAGCTTGAAGAACTTGTTATGAGCAATTTGGACACAATTTTCAAGAGTGCAATTAAAAAGATTGTTGCTTGTGGTTACACCGAAGACGTTGCTACAAAGGCTGTTTTGCGGTCTGGTCTTTGTTATGGGTCCAAGGACACTGTGTCTAATATAGTGGATAATACTTTAGTTTTTCTTAGGAGTGGCCAAGAAATTGATCCTTCAAGGGAGCACTGTTTTGAAGATTTACAGCAGCTAGAGAAGTACATATTGGCGGAACTGGTGTGTGTTCTTCGCGAGATTAGGCCTTTCTTCAGCACTGGCGATGCAATGTGGTGCTTATTGATCTGTGACATGAATGTCTCTCATGCTTGTGCAATGGATGGTGACCCCATAAGCAGTTTCCTCAGTGATGGCTTGTCAAATGGAAGTTCACCCATTTCAAATCAACCCCAGCCCAAAACAGAAGCCAAGAGTTTAGAATTGGGTCTTCTGAATGCTGGTAAGCCGATCTCATCAATGCCTGGTTCTCCTAGTTCACAGTCTGAGACATCTAAACCAAGAAATTCAGTTAACAATGGATTACTCTTAGAAAAAGAGGGCACAACCGGAACATCTCCATCTCCTGCTGTAGAGGAAAAGCTTGTGGGCACTAGAAAGGTCCATTCTATTAGTACTAAAAGAGAATACATGCTTCGACAAAAGTCACTTCATTTGGAGAAAAATTACCGTACATATGGATGTAAAGGGTCTTCAAGAGCAGGGAAGCTGAGTGGTTTGGGCGGTTTAATTTTGGATAAGAAGTTAAAATCTGTGTCAGACTCTACTGCTCTTAATTTAAAGAATGCATCTTTGAAAATAAGCAAGGCGATGGGAGTTGATTTGCCTAAAGACAATGGGAACCACATTCTTTCTTCCAATGCTGGACCTTCTTCCCCCGGAGTTTTTAACGTGGATGCTGAGAATGCTGCTTCAGTTTTGCCTATGAACAGTGTCTCATCCATGTTACCTTCTGTGAATAATTCAACAACATTACCGGCCCCCATTACTGCAAAAACATTGCCAGCAGCCACTACTCCACCTGCATTATCAGCTGCTGATACTGAGCTTTCCCTTTCACTGCCTACAAAAAGCAACACTACCCCAGTGCCTGTTAGCTTTAATTCTGATAATTCAAATTCTATTTTTGCTGGGATACCTTTTGATAAGTCCTTGGGGCAGTGGGTACCCAGGGACAAAAAAGATGAGATGATTTTGAAGTTGGGCCCTAGGGTGCGGGACCTGCAAAATCAGCTGCAAGAGTGGACAGAGTGGGCTAACCAGAAGGTTATGCAGGCTGCCCGTAGGCTAGGTAAGGACAAGGCTGAACTTAAGTCATTAAGGCAAGAGAAAGAAGAAGTTGAACGGCTGAAGAAAGAAAAACTAACGTTGGAGGAGAACACCATGAAGAAACTCGCTGAGATGGACAATGCTTTGTGCAAGGCTAGCGGTCAGGTGGAAAAAGCAAATTCTGCCGTCCGGAGGCTTGAGGTGGAGAATGCTGCACTAAGGCAGGAGATGGAGGCTGCCAAGTTACGCGCGGCAGAGTCAGCTGCAAGCTGTCAAGAGGTATCAAAGAGGGAGAAGAAGACACTCATGAAATTTCAGTCGTGGGAAAAGCAGAAAGCCTTGTTCAGCGAAGAACTGGTGACAGAAAAACGCAAGTTAAAACAGCTGCTTCAGGAAATAGAGCAAGCCAGAGATCTCAAGGAACAATTAGAGGCTAGATGGCAACAAGAGGAGAAATCAAAGGAAGAACTACTTGAGCAGGCAAGTTCAATAAAAAAAGAAAGGGAACAACTTGAGGCTTCAGCAAAATCCAAGGAGGATCATATCAAACTGAAAGCAGAAAGCAATCTGCAGAAATACAAAGATGATATTCAGAATCTCGATAAAGAAATCTCCCAGTTGAGACTCAAGAGTGATTCTTCAAAGATTGCAGCCCTTCGGAGGGGTGTAGATGGAAGTTATGCCAGCAAGGTATCAGACGTTGAAAATAGTCTGGATCAGAAGGGCTCCCAGAAGCCATATATCTCAGAAGTAGTGAAGGATTTGCACGACTACTCTGAGACAGGAGGGGTGAAACGCGAAAGGGAGTGTGTGATGTGCCTCTCCGAGGAAATGTCTGTGGTTTTCCTCCCATGTGCCCATCAAGTGGTCTGCAGAACTTGCAATGAGCTCCATGAAAAACAGGGTATGAAGGACTGTCCATCGTGCAGGAGCCCCATACAATGGCGTATTTCTGTACGTTATGCTCGATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

888

Amino Acids

98.11

Weight (kDa)

7.13

Isoelectric Point (pI)

51.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIR2-like_helical	PF20235	137 - 249	5.2e-31	PIR2-like, helical domain
zf-C3HC4_3	PF13920	831 - 878	5.4e-12	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008859)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g16660 FvH4_4g16660 FvH4_4g16660 FvH4_4g16660
malus_domestica	MD13G1177600.v1.1 MD16G1179600.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15320
rosa_chinensis	RchiOBHm_Chr4g0420121
rosa_laevigata	RLG00000007787
rosa_multiflora	Rmu_sc0015433.1_g000001 Rmu_sc0023895.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362830
rosa_rugosa	Rorug04G0162300 Rorug04G0162400
rosa_samantha	Rh4AG223200 Rh4BG225600 Rh4CG236400 Rh4DG222000
rosa_wichuraiana	Rw4G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1453
AasI	GACNNNNNNGTC	1 cut(s)	722
Acc36I	ACCTGC	2 cut(s)	1453, 1653
Acc65I	GGTACC	1 cut(s)	1588
AccB1I	GGYRCC	1 cut(s)	1588
AccB7I	CCANNNNNTGG	2 cut(s)	2557, 2634
AccII	CGCG	4 cut(s)	660, 1941, 1943, 2490
AccIII	TCCGGA	1 cut(s)	1881
AciI	CCGC	7 cut(s)	176, 493, 638, 1146, 1639, 1851, 1943
AclI	AACGTT	1 cut(s)	1790
AclWI	GGATC	4 cut(s)	98, 575, 2238, 2415
AcoI	YGGCCR	1 cut(s)	568
AcsI	RAATTY	6 cut(s)	167, 182, 928, 1540, 1870, 2003
AcuI	CTGAAG	5 cut(s)	661, 1320, 1793, 2066, 2196
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	6 cut(s)	34, 629, 1039, 1096, 1590, 2649
AfiI	CCNNNNNNNGG	7 cut(s)	113, 1884, 2352, 2353, 2518, 2557, 2634
AflII	CTTAAG	1 cut(s)	1733
AhdI	GACNNNNNGTC	1 cut(s)	526
AjnI	CCWGG	2 cut(s)	886, 1592
AjuI	GAANNNNNNNTTGG	2 cut(s)	617, 649
AleI	CACNNNNGTG	1 cut(s)	41
Alw21I	GWGCWC	2 cut(s)	597, 2583
Alw26I	GTCTC	6 cut(s)	51, 730, 905, 1363, 2308, 2465
AlwI	GGATC	4 cut(s)	98, 575, 2238, 2415
AlwNI	CAGNNNCTG	3 cut(s)	222, 1460, 1514
Aor13HI	TCCGGA	1 cut(s)	1881
AoxI	GGCC	6 cut(s)	310, 320, 568, 668, 1401, 1627
ApaI	GGGCCC	1 cut(s)	1631
ApoI	RAATTY	6 cut(s)	167, 182, 928, 1540, 1870, 2003
ArsI	GACNNNNNNTTYG	2 cut(s)	218, 250
Asp700I	GAANNNNTTC	1 cut(s)	348
Asp718I	GGTACC	1 cut(s)	1588
AspA2I	CCTAGG	1 cut(s)	1631
AspLEI	GCGC	1 cut(s)	1943
AspS9I	GGNCC	9 cut(s)	118, 311, 513, 1024, 1289, 1402, 1627, 1628, 1642
AsuC2I	CCSGG	2 cut(s)	98, 1302
AsuHPI	GGTGA	5 cut(s)	188, 761, 789, 2062, 2494
AsuNHI	GCTAGC	1 cut(s)	1847
AvaII	GGWCC	5 cut(s)	118, 513, 1024, 1289, 1642
AvrII	CCTAGG	1 cut(s)	1631
BaeGI	GKGCMC	4 cut(s)	968, 1016, 1631, 2551
BalI	TGGCCA	1 cut(s)	570
BanI	GGYRCC	1 cut(s)	1588
BanII	GRGCYC	4 cut(s)	1631, 2420, 2583, 2627
BarI	GAAGNNNNNNTAC	2 cut(s)	1099, 1131
BbsI	GAAGAC	4 cut(s)	474, 845, 1107, 1997
Bbv12I	GWGCWC	2 cut(s)	597, 2583
BceAI	ACGGC	2 cut(s)	1784, 1862
BcgI	CGANNNNNNTGC	2 cut(s)	455, 489
BciT130I	CCWGG	2 cut(s)	888, 1594
BciVI	GTATCC	1 cut(s)	1551
BcnI	CCSGG	2 cut(s)	98, 1302
BcoDI	GTCTC	6 cut(s)	51, 730, 905, 1363, 2308, 2465
BfaI	CTAG	9 cut(s)	285, 324, 620, 897, 1017, 1632, 1712, 1848, 2127
BfmI	CTRYAG	3 cut(s)	993, 2261, 2563
BfrI	CTTAAG	1 cut(s)	1733
BfuAI	ACCTGC	2 cut(s)	1453, 1653
BfuI	GTATCC	1 cut(s)	1551
BglII	AGATCT	1 cut(s)	2104
BlnI	CCTAGG	1 cut(s)	1631
BmcAI	AGTACT	1 cut(s)	1039
Bme1390I	CCNGG	4 cut(s)	98, 888, 1302, 1594
Bme18I	GGWCC	5 cut(s)	118, 513, 1024, 1289, 1642
BmeRI	GACNNNNNGTC	1 cut(s)	526
BmgT120I	GGNCC	9 cut(s)	118, 311, 513, 1024, 1289, 1402, 1627, 1628, 1642
BmiI	GGNNCC	9 cut(s)	119, 514, 1265, 1404, 1590, 1629, 1643, 2419, 2624
BmrFI	CCNGG	4 cut(s)	98, 888, 1302, 1594
BmrI	ACTGGG	2 cut(s)	1502, 2302
BmsI	GCATC	4 cut(s)	346, 679, 1220, 1309
BmtI	GCTAGC	1 cut(s)	1851
BmuI	ACTGGG	2 cut(s)	1502, 2302
BoxI	GACNNNNGTC	1 cut(s)	1066
BpiI	GAAGAC	4 cut(s)	474, 845, 1107, 1997
BpuEI	CTTGAG	6 cut(s)	24, 1910, 2093, 2186, 2225, 2303
BpuMI	CCSGG	2 cut(s)	98, 1302
BsaHI	GRCGYC	1 cut(s)	5
BsaWI	WCCGGW	2 cut(s)	971, 1881
BsaXI	ACNNNNNCTCC	2 cut(s)	16, 46
Bsc4I	CCNNNNNNNGG	7 cut(s)	113, 1884, 2352, 2353, 2518, 2557, 2634
Bse118I	RCCGGY	2 cut(s)	318, 1399
Bse1I	ACTGG	6 cut(s)	216, 648, 688, 1508, 2052, 2308
Bse3DI	GCAATG	4 cut(s)	699, 747, 1421, 2581
BseAI	TCCGGA	1 cut(s)	1881
BseBI	CCWGG	2 cut(s)	888, 1594
BseGI	GGATG	4 cut(s)	751, 1109, 1324, 1361
BseLI	CCNNNNNNNGG	7 cut(s)	113, 1884, 2352, 2353, 2518, 2557, 2634
BseMI	GCAATG	4 cut(s)	699, 747, 1421, 2581
BseMII	CTCAG	9 cut(s)	294, 784, 900, 1124, 1314, 1458, 1812, 2451, 2460
BseNI	ACTGG	6 cut(s)	216, 648, 688, 1508, 2052, 2308
BseRI	GAGGAG	2 cut(s)	1811, 2156
BseSI	GKGCMC	4 cut(s)	968, 1016, 1631, 2551
BseYI	CCCAGC	3 cut(s)	384, 816, 1553
BsgI	GTGCAG	2 cut(s)	1890, 2638
Bsh1236I	CGCG	4 cut(s)	660, 1941, 1943, 2490
BshFI	GGCC	6 cut(s)	312, 322, 570, 670, 1403, 1629
BshNI	GGYRCC	1 cut(s)	1588
BsiHKAI	GWGCWC	2 cut(s)	597, 2583
BsiSI	CCGG	6 cut(s)	97, 319, 972, 1302, 1400, 1882
BslFI	GGGAC	3 cut(s)	131, 1610, 1655
BslI	CCNNNNNNNGG	7 cut(s)	113, 1884, 2352, 2353, 2518, 2557, 2634
BsmAI	GTCTC	6 cut(s)	51, 730, 905, 1363, 2308, 2465
BsmFI	GGGAC	3 cut(s)	131, 1610, 1655
BsmI	GAATGC	4 cut(s)	865, 1213, 1333, 1906
BsnI	GGCC	6 cut(s)	312, 322, 570, 670, 1403, 1629
Bsp120I	GGGCCC	1 cut(s)	1627
Bsp1286I	GDGCHC	8 cut(s)	597, 968, 1016, 1631, 2420, 2551, 2583, 2627
Bsp13I	TCCGGA	1 cut(s)	1881
Bsp143I	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
Bsp68I	TCGCGA	1 cut(s)	660
BspACI	CCGC	7 cut(s)	176, 493, 638, 1146, 1639, 1851, 1943
BspANI	GGCC	6 cut(s)	312, 322, 570, 670, 1403, 1629
BspCNI	CTCAG	9 cut(s)	295, 783, 901, 1125, 1315, 1459, 1813, 2450, 2461
BspEI	TCCGGA	1 cut(s)	1881
BspFNI	CGCG	4 cut(s)	660, 1941, 1943, 2490
BspHI	TCATGA	1 cut(s)	1998
BspLI	GGNNCC	9 cut(s)	119, 514, 1265, 1404, 1590, 1629, 1643, 2419, 2624
BspMAI	CTGCAG	2 cut(s)	2265, 2567
BspMI	ACCTGC	2 cut(s)	1453, 1653
BspOI	GCTAGC	1 cut(s)	1851
BspPI	GGATC	4 cut(s)	98, 575, 2238, 2415
BspT107I	GGYRCC	1 cut(s)	1588
BspTI	CTTAAG	1 cut(s)	1733
BsrDI	GCAATG	4 cut(s)	699, 747, 1421, 2581
BsrFI	RCCGGY	2 cut(s)	318, 1399
BsrI	ACTGG	6 cut(s)	216, 648, 688, 1508, 2052, 2308
BssAI	RCCGGY	2 cut(s)	318, 1399
BssMI	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	6 cut(s)	18, 274, 516, 1575, 1631, 2223
Bst2UI	CCWGG	2 cut(s)	888, 1594
Bst4CI	ACNGT	7 cut(s)	61, 526, 599, 906, 1094, 1355, 2609
Bst6I	CTCTTC	1 cut(s)	339
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1733
BstAPI	GCANNNNNTGC	2 cut(s)	1874, 2571
BstC8I	GCNNGC	8 cut(s)	320, 1428, 1698, 1849, 1960, 2097, 2172, 2377
BstEII	GGTNACC	1 cut(s)	749
BstENI	CCTNNNNNAGG	1 cut(s)	2516
BstF5I	GGATG	4 cut(s)	751, 1109, 1324, 1361
BstFNI	CGCG	4 cut(s)	660, 1941, 1943, 2490
BstHHI	GCGC	1 cut(s)	1943
BstKTI	GATC	9 cut(s)	106, 373, 583, 711, 876, 2107, 2233, 2410, 2663
BstMAI	GTCTC	6 cut(s)	51, 730, 905, 1363, 2308, 2465
BstMBI	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
BstMWI	GCNNNNNNNGC	9 cut(s)	482, 1328, 1454, 1645, 1654, 1848, 1874, 1952, 2571
BstNI	CCWGG	2 cut(s)	888, 1594
BstNSI	RCATGY	1 cut(s)	1057
BstPAI	GACNNNNGTC	1 cut(s)	1066
BstPI	GGTNACC	1 cut(s)	749
BstSCI	CCNGG	4 cut(s)	96, 886, 1300, 1592
BstSFI	CTRYAG	3 cut(s)	993, 2261, 2563
BstSLI	GKGCMC	4 cut(s)	968, 1016, 1631, 2551
BstUI	CGCG	4 cut(s)	660, 1941, 1943, 2490
BstV2I	GAAGAC	4 cut(s)	474, 845, 1107, 1997
BstX2I	RGATCY	1 cut(s)	2104
BstXI	CCANNNNNNTGG	2 cut(s)	206, 257
BstYI	RGATCY	1 cut(s)	2104
BsuI	GTATCC	1 cut(s)	1551
BsuRI	GGCC	6 cut(s)	312, 322, 570, 670, 1403, 1629
BtgZI	GCGATG	2 cut(s)	702, 1247
BtsCI	GGATG	4 cut(s)	751, 1109, 1324, 1361
BtsI	GCAGTG	2 cut(s)	1481, 1589
BtsIMutI	CAGTG	9 cut(s)	223, 522, 595, 681, 778, 1360, 1481, 1515, 1589
BtuMI	TCGCGA	1 cut(s)	660
BveI	ACCTGC	2 cut(s)	1453, 1653
Cac8I	GCNNGC	8 cut(s)	320, 1428, 1698, 1849, 1960, 2097, 2172, 2377
CaiI	CAGNNNCTG	3 cut(s)	222, 1460, 1514
CciI	TCATGA	1 cut(s)	1998
CfoI	GCGC	1 cut(s)	1943
Cfr10I	RCCGGY	2 cut(s)	318, 1399
Cfr13I	GGNCC	9 cut(s)	118, 311, 513, 1024, 1289, 1402, 1627, 1628, 1642
Csp6I	GTAC	6 cut(s)	33, 628, 1038, 1095, 1589, 2648
CviAII	CATG	9 cut(s)	206, 718, 731, 1054, 1366, 1807, 1999, 2544, 2585
CviQI	GTAC	6 cut(s)	33, 628, 1038, 1095, 1589, 2648
DpnI	GATC	9 cut(s)	105, 372, 582, 710, 875, 2106, 2232, 2409, 2662
DpnII	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
DraI	TTTAAA	1 cut(s)	1203
DrdI	GACNNNNNNGTC	1 cut(s)	722
DriI	GACNNNNNGTC	1 cut(s)	526
DseDI	GACNNNNNNGTC	1 cut(s)	722
EaeI	YGGCCR	1 cut(s)	568
Eam1104I	CTCTTC	1 cut(s)	339
Eam1105I	GACNNNNNGTC	1 cut(s)	526
EarI	CTCTTC	1 cut(s)	339
EciI	GGCGGA	1 cut(s)	653
Ecl136II	GAGCTC	1 cut(s)	2581
Eco130I	CCWWGG	6 cut(s)	18, 274, 516, 1575, 1631, 2223
Eco147I	AGGCCT	1 cut(s)	670
Eco24I	GRGCYC	4 cut(s)	1631, 2420, 2583, 2627
Eco47I	GGWCC	5 cut(s)	118, 513, 1024, 1289, 1642
Eco53kI	GAGCTC	1 cut(s)	2581
Eco57I	CTGAAG	5 cut(s)	661, 1320, 1793, 2066, 2196
Eco91I	GGTNACC	1 cut(s)	749
EcoICRI	GAGCTC	1 cut(s)	2581
EcoNI	CCTNNNNNAGG	1 cut(s)	2516
EcoO109I	RGGNCCY	2 cut(s)	1628, 1642
EcoO65I	GGTNACC	1 cut(s)	749
EcoRII	CCWGG	2 cut(s)	886, 1592
EcoT14I	CCWWGG	6 cut(s)	18, 274, 516, 1575, 1631, 2223
EcoT22I	ATGCAT	1 cut(s)	1213
EcoT38I	GRGCYC	4 cut(s)	1631, 2420, 2583, 2627
ErhI	CCWWGG	6 cut(s)	18, 274, 516, 1575, 1631, 2223
FaeI	CATG	9 cut(s)	209, 721, 734, 1057, 1369, 1810, 2002, 2547, 2588
FalI	AAGNNNNNCTT	2 cut(s)	2148, 2180
FaqI	GGGAC	3 cut(s)	131, 1610, 1655
FatI	CATG	9 cut(s)	205, 717, 730, 1053, 1365, 1806, 1998, 2543, 2584
FauI	CCCGC	1 cut(s)	1632
FauNDI	CATATG	1 cut(s)	1099
FokI	GGATG	4 cut(s)	758, 1116, 1331, 1348
FriOI	GRGCYC	4 cut(s)	1631, 2420, 2583, 2627
FspBI	CTAG	9 cut(s)	285, 324, 620, 897, 1017, 1632, 1712, 1848, 2127
GlaI	GCGC	1 cut(s)	1942
GsaI	CCCAGC	3 cut(s)	388, 820, 1557
HaeIII	GGCC	6 cut(s)	312, 322, 570, 670, 1403, 1629
HapII	CCGG	6 cut(s)	97, 319, 972, 1302, 1400, 1882
HhaI	GCGC	1 cut(s)	1943
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	209, 721, 734, 1057, 1369, 1810, 2002, 2547, 2588
Hin6I	GCGC	1 cut(s)	1941
HinP1I	GCGC	1 cut(s)	1941
HincII	GTYRAC	1 cut(s)	937
HindII	GTYRAC	1 cut(s)	937
HindIII	AAGCTT	1 cut(s)	1004
HinfI	GANTC	7 cut(s)	202, 332, 1184, 1949, 2287, 2316, 2327
HpaI	GTTAAC	1 cut(s)	937
HpaII	CCGG	6 cut(s)	97, 319, 972, 1302, 1400, 1882
HphI	GGTGA	5 cut(s)	188, 761, 789, 2062, 2494
Hpy166II	GTNNAC	4 cut(s)	797, 902, 937, 1668
Hpy8I	GTNNAC	4 cut(s)	797, 902, 937, 1668
HpyAV	CCTTC	9 cut(s)	321, 594, 1302, 1383, 1681, 2357, 2407, 2443, 2596
HpyCH4III	ACNGT	7 cut(s)	61, 526, 599, 906, 1094, 1355, 2609
HpyCH4IV	ACGT	5 cut(s)	471, 1314, 1790, 2391, 2650
HpyF10VI	GCNNNNNNNGC	9 cut(s)	482, 1328, 1454, 1645, 1654, 1848, 1874, 1952, 2571
HpySE526I	ACGT	5 cut(s)	471, 1314, 1790, 2391, 2650
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	209, 721, 734, 1057, 1369, 1810, 2002, 2547, 2588
HspAI	GCGC	1 cut(s)	1941
Kpn2I	TCCGGA	1 cut(s)	1881
KpnI	GGTACC	1 cut(s)	1592
KroI	GCCGGC	1 cut(s)	318
KroNI	GCCGGC	1 cut(s)	320
KspAI	GTTAAC	1 cut(s)	937
Kzo9I	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
LmnI	GCTCC	4 cut(s)	592, 2423, 2586, 2622
LweI	GCATC	4 cut(s)	346, 679, 1220, 1309
MaeI	CTAG	9 cut(s)	285, 324, 620, 897, 1017, 1632, 1712, 1848, 2127
MaeII	ACGT	5 cut(s)	471, 1314, 1790, 2391, 2650
MalI	GATC	9 cut(s)	105, 372, 582, 710, 875, 2106, 2232, 2409, 2662
MboI	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
MflI	RGATCY	1 cut(s)	2104
MhlI	GDGCHC	8 cut(s)	597, 968, 1016, 1631, 2420, 2551, 2583, 2627
MlsI	TGGCCA	1 cut(s)	570
MluNI	TGGCCA	1 cut(s)	570
MlyI	GAGTC	3 cut(s)	1178, 1958, 2310
MmeI	TCCRAC	1 cut(s)	1773
Mox20I	TGGCCA	1 cut(s)	570
Mph1103I	ATGCAT	1 cut(s)	1213
MroI	TCCGGA	1 cut(s)	1881
MroNI	GCCGGC	1 cut(s)	318
MroXI	GAANNNNTTC	1 cut(s)	348
MscI	TGGCCA	1 cut(s)	570
MslI	CAYNNNNRTG	4 cut(s)	41, 255, 735, 1102
Msp20I	TGGCCA	1 cut(s)	570
MspA1I	CMGCKG	5 cut(s)	176, 1457, 1657, 1955, 2077
MspCI	CTTAAG	1 cut(s)	1733
MspI	CCGG	6 cut(s)	97, 319, 972, 1302, 1400, 1882
MspR9I	CCNGG	4 cut(s)	98, 888, 1302, 1594
Mva1269I	GAATGC	4 cut(s)	865, 1213, 1333, 1906
MvaI	CCWGG	2 cut(s)	888, 1594
MvnI	CGCG	4 cut(s)	660, 1941, 1943, 2490
MwoI	GCNNNNNNNGC	9 cut(s)	482, 1328, 1454, 1645, 1654, 1848, 1874, 1952, 2571
NaeI	GCCGGC	1 cut(s)	320
NciI	CCSGG	2 cut(s)	98, 1302
NdeI	CATATG	1 cut(s)	1099
NdeII	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
NgoMIV	GCCGGC	1 cut(s)	318
NheI	GCTAGC	1 cut(s)	1847
NlaIII	CATG	9 cut(s)	209, 721, 734, 1057, 1369, 1810, 2002, 2547, 2588
NlaIV	GGNNCC	9 cut(s)	119, 514, 1265, 1404, 1590, 1629, 1643, 2419, 2624
NmuCI	GTSAC	6 cut(s)	194, 224, 713, 749, 1068, 2050
NruI	TCGCGA	1 cut(s)	660
NsiI	ATGCAT	1 cut(s)	1213
NspI	RCATGY	1 cut(s)	1057
OliI	CACNNNNGTG	1 cut(s)	41
PagI	TCATGA	1 cut(s)	1998
PaqCI	CACCTGC	1 cut(s)	1453
PasI	CCCWGGG	1 cut(s)	1593
PceI	AGGCCT	1 cut(s)	670
PcsI	WCGNNNNNNNCGW	1 cut(s)	2656
PctI	GAATGC	4 cut(s)	865, 1213, 1333, 1906
PdiI	GCCGGC	1 cut(s)	320
PdmI	GAANNNNTTC	1 cut(s)	348
PfeI	GAWTC	4 cut(s)	202, 332, 2287, 2327
PflMI	CCANNNNNTGG	2 cut(s)	2557, 2634
PleI	GAGTC	3 cut(s)	1178, 1957, 2310
PpsI	GAGTC	3 cut(s)	1178, 1957, 2310
PpuMI	RGGWCCY	1 cut(s)	1642
PshAI	GACNNNNGTC	1 cut(s)	1066
Psp124BI	GAGCTC	1 cut(s)	2583
Psp1406I	AACGTT	1 cut(s)	1790
Psp5II	RGGWCCY	1 cut(s)	1642
Psp6I	CCWGG	2 cut(s)	886, 1592
PspEI	GGTNACC	1 cut(s)	749
PspFI	CCCAGC	3 cut(s)	384, 816, 1553
PspGI	CCWGG	2 cut(s)	886, 1592
PspN4I	GGNNCC	9 cut(s)	119, 514, 1265, 1404, 1590, 1629, 1643, 2419, 2624
PspOMI	GGGCCC	1 cut(s)	1627
PspPI	GGNCC	9 cut(s)	118, 311, 513, 1024, 1289, 1402, 1627, 1628, 1642
PspPPI	RGGWCCY	1 cut(s)	1642
PstI	CTGCAG	2 cut(s)	2265, 2567
PstNI	CAGNNNCTG	3 cut(s)	222, 1460, 1514
PsuI	RGATCY	1 cut(s)	2104
PvuII	CAGCTG	4 cut(s)	1457, 1657, 1955, 2077
RruI	TCGCGA	1 cut(s)	660
RsaI	GTAC	6 cut(s)	34, 629, 1039, 1096, 1590, 2649
RsaNI	GTAC	6 cut(s)	33, 628, 1038, 1095, 1589, 2648
RseI	CAYNNNNRTG	4 cut(s)	41, 255, 735, 1102
SacI	GAGCTC	1 cut(s)	2583
Sau3AI	GATC	9 cut(s)	103, 370, 580, 708, 873, 2104, 2230, 2407, 2660
Sau96I	GGNCC	9 cut(s)	118, 311, 513, 1024, 1289, 1402, 1627, 1628, 1642
ScaI	AGTACT	1 cut(s)	1039
SchI	GAGTC	3 cut(s)	1178, 1958, 2310
ScrFI	CCNGG	4 cut(s)	98, 888, 1302, 1594
SduI	GDGCHC	8 cut(s)	597, 968, 1016, 1631, 2420, 2551, 2583, 2627
SfaNI	GCATC	4 cut(s)	346, 679, 1220, 1309
SfcI	CTRYAG	3 cut(s)	993, 2261, 2563
SinI	GGWCC	5 cut(s)	118, 513, 1024, 1289, 1642
SmiMI	CAYNNNNRTG	4 cut(s)	41, 255, 735, 1102
SmlI	CTYRAG	7 cut(s)	39, 1733, 1889, 2108, 2165, 2204, 2318
SmoI	CTYRAG	7 cut(s)	39, 1733, 1889, 2108, 2165, 2204, 2318
SseBI	AGGCCT	1 cut(s)	670
SsiI	CCGC	7 cut(s)	176, 493, 638, 1146, 1639, 1851, 1943
SspMI	CTAG	9 cut(s)	285, 324, 620, 897, 1017, 1632, 1712, 1848, 2127
SstI	GAGCTC	1 cut(s)	2583
StuI	AGGCCT	1 cut(s)	670
StyD4I	CCNGG	4 cut(s)	96, 886, 1300, 1592
StyI	CCWWGG	6 cut(s)	18, 274, 516, 1575, 1631, 2223
TaaI	ACNGT	7 cut(s)	61, 526, 599, 906, 1094, 1355, 2609
TaiI	ACGT	5 cut(s)	474, 1317, 1793, 2394, 2653
TaqI	TCGA	3 cut(s)	1060, 2292, 2659
TatI	WGTACW	2 cut(s)	627, 1037
TauI	GCSGC	1 cut(s)	1946
TfiI	GAWTC	4 cut(s)	202, 332, 2287, 2327
TscAI	CASTG	9 cut(s)	223, 529, 602, 688, 778, 1360, 1488, 1515, 1589
TseFI	GTSAC	6 cut(s)	194, 224, 713, 749, 1068, 2050
Tsp45I	GTSAC	6 cut(s)	194, 224, 713, 749, 1068, 2050
TspDTI	ATGAA	8 cut(s)	288, 734, 1064, 1364, 1823, 2015, 2601, 2615
TspGWI	ACGGA	1 cut(s)	392
TspRI	CASTG	9 cut(s)	223, 529, 602, 688, 778, 1360, 1488, 1515, 1589
Van91I	CCANNNNNTGG	2 cut(s)	2557, 2634
Vha464I	CTTAAG	1 cut(s)	1733
VpaK11BI	GGWCC	5 cut(s)	118, 513, 1024, 1289, 1642
XagI	CCTNNNNNAGG	1 cut(s)	2516
XapI	RAATTY	6 cut(s)	167, 182, 928, 1540, 1870, 2003
XceI	RCATGY	1 cut(s)	1057
XmaJI	CCTAGG	1 cut(s)	1631
XmnI	GAANNNNTTC	1 cut(s)	348
XspI	CTAG	9 cut(s)	285, 324, 620, 897, 1017, 1632, 1712, 1848, 2127
ZrmI	AGTACT	1 cut(s)	1039
Zsp2I	ATGCAT	1 cut(s)	1213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.