MD13G1182900.v1.1

UPF0613 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008083
Physical Location & Seq
Forward (+)
15400142 .. 15404792
4651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1182900.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGAATCGCTCGCTGTCTGCGTCGTCTTCGTCTTCTTCTCCGTCGTCTTCCTCCGGTTCGTGGTTCTCCGGCGTCGTCCGTGGCCGGTCGGACCGGGCCGGGTCCGTCAAGATGGGAGCAGACTCCCTTTCGGGCGGTTCCGGCGACAATTCCGGCCCCGTTGTGCGGAAGAATCAGTTCCGAGGCGTGCTCTTCAAGTACGGCCCCAAACCTATTCAGGTAGCATTTAAAACAGGTGATTATCGACAGCAAGTCATTTTTATTGGTGGACTGTCTGATGGTTTTCTGGCAACAGAATACTTGGAACCTCTTGCAATTGCTTTGGACAAAGAGAAATGGTCACTTGTTCAACCACTCTTGTCATCTTCATACAGTGGATATGGCACTTCCAGCTTGAAACAAGATGCGGTGGAACTTGATCAGCTGATCAGTCACTTTATAAACAAAGAAGATTCTGAGGGTGTGGTACTGCTTGGCCATAGTACTGGCTGTCAGGATATTGTACATTACATATGCACCAATGCTGCATGCTCCCGAGCAGTCCGTGCTGCCATTCTTCAGGCCCCAGTCAGTGATCGGGAATATAGAGCAACTCTTCCCGAAACAGCTGCTATGATTGACTTGGCTTCAACCATGATTTGTGAAGGACATGGGTCGGAATTAATGCCAAGTGCAGCAGATCCAGGAGCCCCGATAACTGCCTATCGGTATCACTCCCTTTGTGCATACAACGGGGATGATGACATGTTTAGTTCTGACCTTACTGATGACCAGCTGAGGTTGAGACTTGGGCACATGGCTAATACACCTTGTCAGGTTATATTTTCCATGGCTGATGAATATGTGCCAGACTATGTTGACAAGAAAGCACTGGTCCAAAGATTGTGCAAAGCAATGGGTGGTGCTGAGAAAGTTGAAATTGAACACGGGAATCACTCCCTGTCCAACAGAGTTGATGAAGCTGTTCATGCGATCATTGATTTTGTTAAAAGAGACGGACCGAAAGGCTGGGATGATCCGTGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1749

Protein Analysis

343

Amino Acids

36.98

Weight (kDa)

5.46

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1749 PF08538 60 - 325 2.3e-65 Protein of unknown function (DUF1749)
Abhydrolase_6 PF12697 124 - 315 5.6e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 440
AciI CCGC 3 cut(s) 135, 166, 407
AclWI GGATC 2 cut(s) 674, 1010
AcoI YGGCCR 2 cut(s) 82, 475
AcuI CTGAAG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 4 cut(s) 200, 468, 484, 504
AfiI CCNNNNNNNGG 2 cut(s) 60, 165
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 6 cut(s) 196, 350, 397, 630, 917, 923
AhdI GACNNNNNGTC 1 cut(s) 251
AjnI CCWGG 1 cut(s) 682
AluBI AGCT 5 cut(s) 393, 424, 608, 775, 962
AluI AGCT 5 cut(s) 393, 424, 608, 775, 962
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 778, 987
AlwI GGATC 2 cut(s) 674, 1010
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 6 cut(s) 82, 96, 154, 202, 475, 561
ApeKI GCWGC 4 cut(s) 524, 548, 608, 674
AseI ATTAAT 1 cut(s) 662
Asp700I GAANNNNTTC 2 cut(s) 176, 963
AspS9I GGNCC 8 cut(s) 91, 96, 102, 155, 203, 562, 874, 998
AsuC2I CCSGG 2 cut(s) 95, 100
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 534
AvaII GGWCC 4 cut(s) 91, 102, 874, 998
BaeGI GKGCMC 1 cut(s) 795
BalI TGGCCA 1 cut(s) 477
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 3 cut(s) 18, 24, 39
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 776
BbvI GCAGC 4 cut(s) 511, 535, 595, 686
BccI CCATC 2 cut(s) 106, 272
BceAI ACGGC 1 cut(s) 217
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 1 cut(s) 684
BclI TGATCA 2 cut(s) 418, 426
BcnI CCSGG 2 cut(s) 95, 100
BcoDI GTCTC 2 cut(s) 778, 987
BisI GCNGC 4 cut(s) 525, 549, 609, 675
BlsI GCNGC 4 cut(s) 526, 550, 610, 676
BmcAI AGTACT 1 cut(s) 484
Bme1390I CCNGG 3 cut(s) 95, 100, 684
Bme18I GGWCC 4 cut(s) 91, 102, 874, 998
BmeRI GACNNNNNGTC 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 8 cut(s) 91, 96, 102, 155, 203, 562, 874, 998
BmiI GGNNCC 7 cut(s) 103, 139, 157, 205, 306, 564, 688
BmrFI CCNGG 3 cut(s) 95, 100, 684
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 1 cut(s) 394
BmuI ACTGGG 1 cut(s) 560
BoxI GACNNNNGTC 1 cut(s) 652
BpiI GAAGAC 3 cut(s) 18, 24, 39
BplI GAGNNNNNCTC 2 cut(s) 174, 206
Bpu10I CCTNAGC 1 cut(s) 776
BpuMI CCSGG 2 cut(s) 95, 100
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 79, 181, 828, 1019
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 165
Bse118I RCCGGY 1 cut(s) 84
Bse1I ACTGG 3 cut(s) 490, 566, 876
Bse3DI GCAATG 1 cut(s) 900
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 4 cut(s) 79, 181, 828, 1019
BseGI GGATG 2 cut(s) 742, 1018
BseLI CCNNNNNNNGG 2 cut(s) 60, 165
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 3 cut(s) 447, 767, 897
BseNI ACTGG 3 cut(s) 490, 566, 876
BseSI GKGCMC 1 cut(s) 795
BseXI GCAGC 4 cut(s) 511, 535, 595, 686
BseYI CCCAGC 1 cut(s) 1008
BsgI GTGCAG 1 cut(s) 693
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 6 cut(s) 84, 98, 156, 204, 477, 563
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 7 cut(s) 54, 69, 85, 94, 99, 141, 153
BslI CCNNNNNNNGG 2 cut(s) 60, 165
BsmAI GTCTC 2 cut(s) 778, 987
BsmBI CGTCTC 1 cut(s) 987
BsnI GGCC 6 cut(s) 84, 98, 156, 204, 477, 563
BsoBI CYCGRG 1 cut(s) 534
Bsp1286I GDGCHC 3 cut(s) 192, 691, 795
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
Bsp19I CCATGG 1 cut(s) 828
BspACI CCGC 3 cut(s) 135, 166, 407
BspANI GGCC 6 cut(s) 84, 98, 156, 204, 477, 563
BspCNI CTCAG 3 cut(s) 448, 768, 898
BspLI GGNNCC 7 cut(s) 103, 139, 157, 205, 306, 564, 688
BspPI GGATC 2 cut(s) 674, 1010
BspQI GCTCTTC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 900
BsrFI RCCGGY 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 3 cut(s) 490, 566, 876
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 4 cut(s) 79, 181, 828, 1019
BssMI GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 273, 374
Bst6I CTCTTC 2 cut(s) 197, 600
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 502
BstC8I GCNNGC 3 cut(s) 11, 188, 529
BstDEI CTNAG 3 cut(s) 456, 776, 906
BstDSI CCRYGG 3 cut(s) 79, 828, 1019
BstF5I GGATG 2 cut(s) 742, 1018
BstKTI GATC 6 cut(s) 421, 429, 577, 682, 975, 1018
BstMAI GTCTC 2 cut(s) 778, 987
BstMBI GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 4 cut(s) 141, 390, 545, 968
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 2 cut(s) 531, 748
BstPAI GACNNNNGTC 1 cut(s) 652
BstSCI CCNGG 3 cut(s) 93, 98, 682
BstSLI GKGCMC 1 cut(s) 795
BstV1I GCAGC 4 cut(s) 511, 535, 595, 686
BstV2I GAAGAC 3 cut(s) 18, 24, 39
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 1 cut(s) 679
BsuRI GGCC 6 cut(s) 84, 98, 156, 204, 477, 563
BtgI CCRYGG 3 cut(s) 79, 828, 1019
BtsCI GGATG 2 cut(s) 742, 1018
BtsIMutI CAGTG 3 cut(s) 379, 577, 869
Cac8I GCNNGC 3 cut(s) 11, 188, 529
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 8 cut(s) 91, 96, 102, 155, 203, 562, 874, 998
CpoI CGGWCCG 2 cut(s) 91, 998
CseI GACGC 2 cut(s) 9, 61
Csp6I GTAC 4 cut(s) 199, 467, 483, 503
CspI CGGWCCG 2 cut(s) 91, 998
CviAII CATG 7 cut(s) 528, 634, 650, 745, 796, 829, 968
CviQI GTAC 4 cut(s) 199, 467, 483, 503
DdeI CTNAG 3 cut(s) 456, 776, 906
DpnI GATC 6 cut(s) 420, 428, 576, 681, 974, 1017
DpnII GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
DraI TTTAAA 1 cut(s) 229
DriI GACNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 2 cut(s) 82, 475
Eam1104I CTCTTC 2 cut(s) 197, 600
Eam1105I GACNNNNNGTC 1 cut(s) 251
EarI CTCTTC 2 cut(s) 197, 600
Eco130I CCWWGG 1 cut(s) 828
Eco24I GRGCYC 1 cut(s) 691
Eco47I GGWCC 4 cut(s) 91, 102, 874, 998
Eco57I CTGAAG 1 cut(s) 542
Eco88I CYCGRG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 828
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 1 cut(s) 828
Esp3I CGTCTC 1 cut(s) 987
FaeI CATG 7 cut(s) 531, 637, 653, 748, 799, 832, 971
FatI CATG 7 cut(s) 527, 633, 649, 744, 795, 828, 967
FauNDI CATATG 1 cut(s) 512
FbaI TGATCA 2 cut(s) 418, 426
Fnu4HI GCNGC 4 cut(s) 525, 549, 609, 675
FokI GGATG 2 cut(s) 749, 1025
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 4 cut(s) 525, 549, 609, 675
GluI GCNGC 4 cut(s) 525, 549, 609, 675
GsaI CCCAGC 1 cut(s) 1012
HaeIII GGCC 6 cut(s) 84, 98, 156, 204, 477, 563
HapII CCGG 7 cut(s) 54, 69, 85, 94, 99, 141, 153
HgaI GACGC 2 cut(s) 9, 61
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 7 cut(s) 531, 637, 653, 748, 799, 832, 971
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 5 cut(s) 4, 122, 172, 452, 931
HpaII CCGG 7 cut(s) 54, 69, 85, 94, 99, 141, 153
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 2 cut(s) 269, 859
Hpy188I TCNGA 6 cut(s) 91, 182, 277, 457, 658, 757
Hpy188III TCNNGA 5 cut(s) 109, 494, 534, 578, 599
Hpy8I GTNNAC 2 cut(s) 269, 859
Hpy99I CGWCG 3 cut(s) 25, 46, 77
HpyAV CCTTC 1 cut(s) 638
HpyCH4III ACNGT 2 cut(s) 273, 374
HpyCH4V TGCA 6 cut(s) 314, 516, 527, 674, 725, 888
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 390, 545, 968
HpyF3I CTNAG 3 cut(s) 456, 776, 906
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 7 cut(s) 531, 637, 653, 748, 799, 832, 971
Ksp22I TGATCA 2 cut(s) 418, 426
Kzo9I GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
LguI GCTCTTC 1 cut(s) 197
LmnI GCTCC 3 cut(s) 116, 536, 686
Lsp1109I GCAGC 4 cut(s) 511, 535, 595, 686
LweI GCATC 1 cut(s) 394
MaeIII GTNAC 2 cut(s) 339, 431
MalI GATC 6 cut(s) 420, 428, 576, 681, 974, 1017
MboI GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 3 cut(s) 192, 691, 795
MlsI TGGCCA 1 cut(s) 477
MluCI AATT 4 cut(s) 148, 315, 659, 918
MluNI TGGCCA 1 cut(s) 477
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 3 cut(s) 69, 636, 969
MnlI CCTC 5 cut(s) 61, 176, 318, 451, 771
Mox20I TGGCCA 1 cut(s) 477
MroXI GAANNNNTTC 2 cut(s) 176, 963
MscI TGGCCA 1 cut(s) 477
MseI TTAA 3 cut(s) 228, 662, 987
Msp20I TGGCCA 1 cut(s) 477
MspA1I CMGCKG 3 cut(s) 424, 608, 775
MspI CCGG 7 cut(s) 54, 69, 85, 94, 99, 141, 153
MspR9I CCNGG 3 cut(s) 95, 100, 684
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 4 cut(s) 141, 390, 545, 968
NciI CCSGG 2 cut(s) 95, 100
NcoI CCATGG 1 cut(s) 828
NdeI CATATG 1 cut(s) 512
NdeII GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
NlaIII CATG 7 cut(s) 531, 637, 653, 748, 799, 832, 971
NlaIV GGNNCC 7 cut(s) 103, 139, 157, 205, 306, 564, 688
NmuCI GTSAC 2 cut(s) 339, 431
NspI RCATGY 2 cut(s) 531, 748
PaeI GCATGC 1 cut(s) 531
PciI ACATGT 1 cut(s) 744
PciSI GCTCTTC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PdmI GAANNNNTTC 2 cut(s) 176, 963
PfeI GAWTC 4 cut(s) 4, 172, 452, 931
PfoI TCCNGGA 1 cut(s) 682
PkrI GCNGC 4 cut(s) 526, 550, 610, 676
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PscI ACATGT 1 cut(s) 744
PshAI GACNNNNGTC 1 cut(s) 652
PshBI ATTAAT 1 cut(s) 662
PsiI TTATAA 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 682
PspFI CCCAGC 1 cut(s) 1008
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 7 cut(s) 103, 139, 157, 205, 306, 564, 688
PspPI GGNCC 8 cut(s) 91, 96, 102, 155, 203, 562, 874, 998
PsuI RGATCY 1 cut(s) 679
PvuII CAGCTG 3 cut(s) 424, 608, 775
RsaI GTAC 4 cut(s) 200, 468, 484, 504
RsaNI GTAC 4 cut(s) 199, 467, 483, 503
Rsr2I CGGWCCG 2 cut(s) 91, 998
RsrII CGGWCCG 2 cut(s) 91, 998
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 228, 662, 987
SatI GCNGC 4 cut(s) 525, 549, 609, 675
Sau3AI GATC 6 cut(s) 418, 426, 574, 679, 972, 1015
Sau96I GGNCC 8 cut(s) 91, 96, 102, 155, 203, 562, 874, 998
ScaI AGTACT 1 cut(s) 484
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 3 cut(s) 95, 100, 684
SduI GDGCHC 3 cut(s) 192, 691, 795
SfaNI GCATC 1 cut(s) 394
SinI GGWCC 4 cut(s) 91, 102, 874, 998
SphI GCATGC 1 cut(s) 531
Sse9I AATT 4 cut(s) 148, 315, 659, 918
SsiI CCGC 3 cut(s) 135, 166, 407
StyD4I CCNGG 3 cut(s) 93, 98, 682
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 2 cut(s) 273, 374
TaqI TCGA 1 cut(s) 244
TaqII GACCGA 1 cut(s) 1015
TasI AATT 4 cut(s) 148, 315, 659, 918
TatI WGTACW 2 cut(s) 482, 502
TfiI GAWTC 4 cut(s) 4, 172, 452, 931
Tru1I TTAA 3 cut(s) 228, 662, 987
Tru9I TTAA 3 cut(s) 228, 662, 987
TscAI CASTG 3 cut(s) 379, 577, 876
TseFI GTSAC 2 cut(s) 339, 431
TseI GCWGC 4 cut(s) 524, 548, 608, 674
Tsp45I GTSAC 2 cut(s) 339, 431
TspDTI ATGAA 5 cut(s) 17, 357, 852, 956, 972
TspGWI ACGGA 6 cut(s) 30, 68, 94, 533, 1008, 1011
TspRI CASTG 3 cut(s) 379, 577, 876
VpaK11BI GGWCC 4 cut(s) 91, 102, 874, 998
VspI ATTAAT 1 cut(s) 662
XceI RCATGY 2 cut(s) 531, 748
XmnI GAANNNNTTC 2 cut(s) 176, 963
ZrmI AGTACT 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.