Rh4DG212700

UPF0613 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008083
Physical Location & Seq
Reverse (-)
40789755 .. 40794079
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG212700.1

Sequence Viewer

Length: 1056 bp
ATGAATCCCTCGCTGTCTTCTTCTTCTTCTTCTCCTCCGTCTTCTACTTCGGCTTCCGCCGGTTCCTGGTTCTCCGGCGTTGTCCGGGGCCGGTCCGACCGGGCCGGGTCCGTCAAGATGGGCTCCGACTCTGGCTCCGGTGGTTCCGGTGACTTTTCCGGCCCCGTCGTGCGCAAGAATCAGTTCCGAGGGGCGCTCTTCAAGTACGGCCCCAAACCAATTCAGGTTGCATTTAAAACAGGTGATTATAGACAGCAAGTCATCTTTATTGGTGGATTGACCGATGGCTTTCTGGCAACAGAATACTTGGAACCTCTTGCAATTGCTTTGGACAAGGAGAAATGGTCACTTGTTCAACTACTCATGTCATCTTCATACAATGGATATGGCACTTCCAGCTTGAAACAAGATGCGATGGAACTTGATCAGCTGATTAGTTATTTTATAAACAAAGAAGATTCTGAGGGTGTTGTACTGATTGGCCATAGTACTGGCTGCCAGGACATTGTACATTATATACGCACAAATGCTGCATGCTCTAGAGCGGTTCGTGCTGCCATTCTACAGCATAACACCAATCAAATTGTTCAGGCTCCAGTCAGTGATCGGGAATACAGAGCAACTCTTCCTGAAACAGCTACTATGATTGACTTGGCTTCAACCATGATAAATGAAGGTCGCGGATCTCAATTAATGCCAAGTGAAGCAGATTTAGCCCCAATAACTGCCTATCGGTATCACTCACTTTGTGCATACAACGGGGATGATGACATGTTTAGTTCTGACCTTACTGATGACCAGCTGAGACTGAAACTTGGGCACATGACTAACACACCTTGCCAGGTTATTTATTCCATGGCTGATGAGTATGTGCCAGACTATGTTGACAAGAAAGCATTGGTTCAAAGATTGTGCAAAGCAATGGGTGGTGCAGAAAAGGTTGAAATTGAACATGGAAATCACTCCCTTTCTAACAGAGTTCATGAAGCTGTCGATGCGATGATTGATTTTGTTAAGAGAGACGGACCTAAAGGGTGGGATGATCCATGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1749

Protein Analysis

351

Amino Acids

38.28

Weight (kDa)

5.4

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1749 PF08538 63 - 335 4.8e-65 Protein of unknown function (DUF1749)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
Acc16I TGCGCA 1 cut(s) 173
AccBSI CCGCTC 1 cut(s) 545
AccII CGCG 1 cut(s) 681
AciI CCGC 3 cut(s) 57, 545, 681
AclWI GGATC 2 cut(s) 691, 1037
AcoI YGGCCR 1 cut(s) 481
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 4 cut(s) 206, 474, 490, 510
AfiI CCNNNNNNNGG 1 cut(s) 66
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 7 cut(s) 202, 356, 403, 660, 905, 944, 950
AhdI GACNNNNNGTC 1 cut(s) 257
AjnI CCWGG 3 cut(s) 65, 498, 840
AluBI AGCT 5 cut(s) 399, 430, 638, 802, 989
AluI AGCT 5 cut(s) 399, 430, 638, 802, 989
Alw26I GTCTC 2 cut(s) 799, 1014
AlwI GGATC 2 cut(s) 691, 1037
AoxI GGCC 5 cut(s) 88, 102, 160, 208, 481
ApeKI GCWGC 3 cut(s) 495, 530, 554
AseI ATTAAT 1 cut(s) 692
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 2 cut(s) 174, 196
AspS9I GGNCC 7 cut(s) 88, 93, 102, 108, 161, 209, 1025
AsuC2I CCSGG 3 cut(s) 86, 101, 106
AsuHPI GGTGA 2 cut(s) 161, 254
AvaII GGWCC 3 cut(s) 93, 108, 1025
BaeGI GKGCMC 1 cut(s) 822
BalI TGGCCA 1 cut(s) 483
BanII GRGCYC 1 cut(s) 125
BbsI GAAGAC 2 cut(s) 9, 33
BbvI GCAGC 3 cut(s) 482, 517, 541
BccI CCATC 3 cut(s) 112, 278, 409
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 3 cut(s) 67, 500, 842
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 3 cut(s) 86, 101, 106
BcoDI GTCTC 2 cut(s) 799, 1014
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 197
BisI GCNGC 3 cut(s) 496, 531, 555
BlsI GCNGC 3 cut(s) 497, 532, 556
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 6 cut(s) 67, 86, 101, 106, 500, 842
Bme18I GGWCC 3 cut(s) 93, 108, 1025
BmeRI GACNNNNNGTC 1 cut(s) 257
BmgT120I GGNCC 7 cut(s) 88, 93, 102, 108, 161, 209, 1025
BmrFI CCNGG 6 cut(s) 67, 86, 101, 106, 500, 842
BmsI GCATC 2 cut(s) 400, 985
BpiI GAAGAC 2 cut(s) 9, 33
BplI GAGNNNNNCTC 2 cut(s) 180, 212
BpmI CTGGAG 1 cut(s) 579
BpuMI CCSGG 3 cut(s) 86, 101, 106
BsaJI CCNNGG 4 cut(s) 85, 187, 855, 1046
BsaWI WCCGGW 2 cut(s) 137, 146
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse118I RCCGGY 2 cut(s) 59, 90
Bse1I ACTGG 2 cut(s) 496, 596
Bse3DI GCAATG 1 cut(s) 927
BseBI CCWGG 3 cut(s) 67, 500, 842
BseDI CCNNGG 4 cut(s) 85, 187, 855, 1046
BseGI GGATG 2 cut(s) 769, 1045
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 2 cut(s) 453, 794
BseNI ACTGG 2 cut(s) 496, 596
BseRI GAGGAG 1 cut(s) 24
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 3 cut(s) 482, 517, 541
BsgI GTGCAG 1 cut(s) 951
Bsh1236I CGCG 1 cut(s) 681
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 5 cut(s) 90, 104, 162, 210, 483
BsiEI CGRYCG 1 cut(s) 100
BsiSI CCGG 9 cut(s) 60, 75, 85, 91, 100, 105, 138, 147, 159
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 2 cut(s) 799, 1014
BsmBI CGTCTC 1 cut(s) 1014
BsnI GGCC 5 cut(s) 90, 104, 162, 210, 483
Bsp1286I GDGCHC 2 cut(s) 125, 822
Bsp1407I TGTACA 1 cut(s) 508
Bsp143I GATC 4 cut(s) 424, 604, 683, 1042
Bsp19I CCATGG 2 cut(s) 855, 1046
BspACI CCGC 3 cut(s) 57, 545, 681
BspANI GGCC 5 cut(s) 90, 104, 162, 210, 483
BspCNI CTCAG 2 cut(s) 454, 795
BspFNI CGCG 1 cut(s) 681
BspHI TCATGA 1 cut(s) 982
BspPI GGATC 2 cut(s) 691, 1037
BspQI GCTCTTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 2 cut(s) 59, 90
BsrGI TGTACA 1 cut(s) 508
BsrI ACTGG 2 cut(s) 496, 596
BssAI RCCGGY 2 cut(s) 59, 90
BssECI CCNNGG 4 cut(s) 85, 187, 855, 1046
BssMI GATC 4 cut(s) 424, 604, 683, 1042
BssT1I CCWWGG 2 cut(s) 855, 1046
Bst2UI CCWGG 3 cut(s) 67, 500, 842
Bst6I CTCTTC 2 cut(s) 203, 630
BstAUI TGTACA 1 cut(s) 508
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 2 cut(s) 462, 803
BstDSI CCRYGG 2 cut(s) 855, 1046
BstF5I GGATG 2 cut(s) 769, 1045
BstFNI CGCG 1 cut(s) 681
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 2 cut(s) 174, 196
BstKTI GATC 4 cut(s) 427, 607, 686, 1045
BstMAI GTCTC 2 cut(s) 799, 1014
BstMBI GATC 4 cut(s) 424, 604, 683, 1042
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 4 cut(s) 396, 551, 713, 995
BstNI CCWGG 3 cut(s) 67, 500, 842
BstNSI RCATGY 2 cut(s) 537, 775
BstSCI CCNGG 6 cut(s) 65, 84, 99, 104, 498, 840
BstSFI CTRYAG 1 cut(s) 563
BstSLI GKGCMC 1 cut(s) 822
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 3 cut(s) 482, 517, 541
BstV2I GAAGAC 2 cut(s) 9, 33
BstX2I RGATCY 1 cut(s) 683
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 683
BsuRI GGCC 5 cut(s) 90, 104, 162, 210, 483
BtgI CCRYGG 2 cut(s) 855, 1046
BtgZI GCGATG 2 cut(s) 428, 1013
BtsCI GGATG 2 cut(s) 769, 1045
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 535
CciI TCATGA 1 cut(s) 982
CfoI GCGC 2 cut(s) 174, 196
Cfr10I RCCGGY 2 cut(s) 59, 90
Cfr13I GGNCC 7 cut(s) 88, 93, 102, 108, 161, 209, 1025
CpoI CGGWCCG 1 cut(s) 93
Csp6I GTAC 4 cut(s) 205, 473, 489, 509
CspI CGGWCCG 1 cut(s) 93
CviAII CATG 9 cut(s) 364, 534, 664, 772, 823, 856, 953, 983, 1047
CviQI GTAC 4 cut(s) 205, 473, 489, 509
DdeI CTNAG 2 cut(s) 462, 803
DpnI GATC 4 cut(s) 426, 606, 685, 1044
DpnII GATC 4 cut(s) 424, 604, 683, 1042
DraI TTTAAA 1 cut(s) 235
DraIII CACNNNGTG 1 cut(s) 749
DriI GACNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 2 cut(s) 203, 630
Eam1105I GACNNNNNGTC 1 cut(s) 257
EarI CTCTTC 2 cut(s) 203, 630
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 855, 1046
Eco24I GRGCYC 1 cut(s) 125
Eco47I GGWCC 3 cut(s) 93, 108, 1025
EcoRII CCWGG 3 cut(s) 65, 498, 840
EcoT14I CCWWGG 2 cut(s) 855, 1046
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 2 cut(s) 855, 1046
Esp3I CGTCTC 1 cut(s) 1014
FaeI CATG 9 cut(s) 367, 537, 667, 775, 826, 859, 956, 986, 1050
FatI CATG 9 cut(s) 363, 533, 663, 771, 822, 855, 952, 982, 1046
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 3 cut(s) 496, 531, 555
FokI GGATG 2 cut(s) 776, 1052
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 3 cut(s) 496, 531, 555
FspBI CTAG 1 cut(s) 540
FspI TGCGCA 1 cut(s) 173
GlaI GCGC 2 cut(s) 173, 195
GluI GCNGC 3 cut(s) 496, 531, 555
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 197
HaeIII GGCC 5 cut(s) 90, 104, 162, 210, 483
HapII CCGG 9 cut(s) 60, 75, 85, 91, 100, 105, 138, 147, 159
HhaI GCGC 2 cut(s) 174, 196
Hin1II CATG 9 cut(s) 367, 537, 667, 775, 826, 859, 956, 986, 1050
Hin6I GCGC 2 cut(s) 172, 194
HinP1I GCGC 2 cut(s) 172, 194
HincII GTYRAC 1 cut(s) 886
HindII GTYRAC 1 cut(s) 886
HinfI GANTC 4 cut(s) 4, 128, 178, 458
HpaII CCGG 9 cut(s) 60, 75, 85, 91, 100, 105, 138, 147, 159
HphI GGTGA 2 cut(s) 161, 254
Hpy166II GTNNAC 1 cut(s) 886
Hpy188I TCNGA 5 cut(s) 97, 127, 188, 463, 784
Hpy188III TCNNGA 5 cut(s) 115, 540, 608, 629, 983
Hpy8I GTNNAC 1 cut(s) 886
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 668
HpyCH4V TGCA 6 cut(s) 230, 320, 533, 752, 915, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 396, 551, 713, 995
HpyF3I CTNAG 2 cut(s) 462, 803
Hsp92II CATG 9 cut(s) 367, 537, 667, 775, 826, 859, 956, 986, 1050
HspAI GCGC 2 cut(s) 172, 194
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 4 cut(s) 424, 604, 683, 1042
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 3 cut(s) 128, 140, 598
Lsp1109I GCAGC 3 cut(s) 482, 517, 541
LweI GCATC 2 cut(s) 400, 985
MaeI CTAG 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 149, 345
MalI GATC 4 cut(s) 426, 606, 685, 1044
MbiI CCGCTC 1 cut(s) 545
MboI GATC 4 cut(s) 424, 604, 683, 1042
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 2 cut(s) 125, 822
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 6 cut(s) 219, 321, 582, 689, 945, 1051
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 120, 150
MnlI CCTC 5 cut(s) 19, 45, 182, 324, 457
Mox20I TGGCCA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 182
MscI TGGCCA 1 cut(s) 483
MseI TTAA 3 cut(s) 234, 692, 1014
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 430, 802
MspI CCGG 9 cut(s) 60, 75, 85, 91, 100, 105, 138, 147, 159
MspR9I CCNGG 6 cut(s) 67, 86, 101, 106, 500, 842
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 3 cut(s) 67, 500, 842
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 4 cut(s) 396, 551, 713, 995
NciI CCSGG 3 cut(s) 86, 101, 106
NcoI CCATGG 2 cut(s) 855, 1046
NdeII GATC 4 cut(s) 424, 604, 683, 1042
NlaIII CATG 9 cut(s) 367, 537, 667, 775, 826, 859, 956, 986, 1050
NmuCI GTSAC 2 cut(s) 149, 345
NsbI TGCGCA 1 cut(s) 173
NspI RCATGY 2 cut(s) 537, 775
PaeI GCATGC 1 cut(s) 537
PagI TCATGA 1 cut(s) 982
PciI ACATGT 1 cut(s) 771
PciSI GCTCTTC 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 3 cut(s) 4, 178, 458
PkrI GCNGC 3 cut(s) 497, 532, 556
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PscI ACATGT 1 cut(s) 771
PshBI ATTAAT 1 cut(s) 692
PsiI TTATAA 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 65, 498, 840
PspGI CCWGG 3 cut(s) 65, 498, 840
PspPI GGNCC 7 cut(s) 88, 93, 102, 108, 161, 209, 1025
PsuI RGATCY 1 cut(s) 683
PvuII CAGCTG 2 cut(s) 430, 802
RsaI GTAC 4 cut(s) 206, 474, 490, 510
RsaNI GTAC 4 cut(s) 205, 473, 489, 509
Rsr2I CGGWCCG 1 cut(s) 93
RsrII CGGWCCG 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 3 cut(s) 234, 692, 1014
SatI GCNGC 3 cut(s) 496, 531, 555
Sau3AI GATC 4 cut(s) 424, 604, 683, 1042
Sau96I GGNCC 7 cut(s) 88, 93, 102, 108, 161, 209, 1025
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 6 cut(s) 67, 86, 101, 106, 500, 842
SduI GDGCHC 2 cut(s) 125, 822
SfaNI GCATC 2 cut(s) 400, 985
SfcI CTRYAG 1 cut(s) 563
SinI GGWCC 3 cut(s) 93, 108, 1025
SphI GCATGC 1 cut(s) 537
Sse9I AATT 6 cut(s) 219, 321, 582, 689, 945, 1051
SsiI CCGC 3 cut(s) 57, 545, 681
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 6 cut(s) 65, 84, 99, 104, 498, 840
StyI CCWWGG 2 cut(s) 855, 1046
TaqI TCGA 1 cut(s) 993
TaqII GACCGA 1 cut(s) 296
TasI AATT 6 cut(s) 219, 321, 582, 689, 945, 1051
TatI WGTACW 3 cut(s) 472, 488, 508
TfiI GAWTC 3 cut(s) 4, 178, 458
Tru1I TTAA 3 cut(s) 234, 692, 1014
Tru9I TTAA 3 cut(s) 234, 692, 1014
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 2 cut(s) 149, 345
TseI GCWGC 3 cut(s) 495, 530, 554
Tsp45I GTSAC 2 cut(s) 149, 345
TspDTI ATGAA 5 cut(s) 17, 363, 687, 971, 999
TspGWI ACGGA 3 cut(s) 27, 100, 1038
TspRI CASTG 1 cut(s) 607
VpaK11BI GGWCC 3 cut(s) 93, 108, 1025
VspI ATTAAT 1 cut(s) 692
XbaI TCTAGA 1 cut(s) 539
XceI RCATGY 2 cut(s) 537, 775
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.