Rh4CG225900

UPF0613 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008083
Physical Location & Seq
Reverse (-)
46218897 .. 46224024
5128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG225900.1

Sequence Viewer

Length: 1023 bp
ATGAATCCCTCGCTGTCTTCTTCTCCTCCGTCTTCTACTTCGGCTTCCGCCGGTTCCTGGTTCTCCGGCGTTGTCCGGGGCCGGTCCGACCGGGCCGGGTCCGTCAAGATGGGCTCCGACTCTGGCTCTGGTGGTTCCGGTGACTTTTCCGGCCCCGTCGTGCGGAAGAATCAGTTCCGAGGGGCGCTCTTCAAGTACGGCCCCAAACCAATTCAGGTTGCATTTAAAACAGGTGATTATAGACAGCAAGTCATCTTTATTGGTGGATTGACCGATGGCTTTCTGGCAACAGAATACTTGGAACCTCTTGCAATTGCTTTGGACAAGGAGAAATGGTCACTTGTTCAACTACTCATGTCATCTTCATACAATGGATATGGCACTTCCAGCTTGAAACAAGACGCGATGGAACTTGATCAGCTGATTAGTTATTTTATAAACAAAGAAGATTCTGAGGGTGTTGTACTGATTGGCCATAGTACTGGCTGCCAGGACATTGTACATTATATACGCACAAATGCTGCATGCTCTAGAGCGGTTCGTGCTGCCATTCTACAGGCTCCAGTCAGTGATAGGGAATACAGAGCAACTCTTCCTGAAACAGCTGCTATGATTGACTTGGCTTCAACCATGATAAATGAAGGTCACGGATCTCAATTAATGCCAAGTGAAGCAGATTTAGCCCCAATAACTGCCTATCGGTATCACTCACTTTGTGCATACAACGGGGATGATGACATGTTTAGTTCTGACCTTACTGATGACCAGCTGAGACTGAAACTTGGGCACATGACTAACACACCTTGCCAGGTTATTTATTCCATGGCTGATGAGTATGTGCCAGACTATGTTGACAAGAAAGCATTGGTTCAAAGATTGTGCAAAGCAATGGGTGGTGCAGAAAAGGTTGAAATTGAACATGGAAATCACTCCCTTTCTAACAGAGTTCATGAAGCTGTCGATGCGATGATTGATTTTGTTAAGAGAGACGGGCCTAAAGGGTGGGATGATCCATGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1749

Protein Analysis

340

Amino Acids

37.04

Weight (kDa)

5.29

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1749 PF08538 60 - 324 1.9e-67 Protein of unknown function (DUF1749)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 536
AccII CGCG 1 cut(s) 404
AciI CCGC 3 cut(s) 48, 163, 536
AclWI GGATC 2 cut(s) 658, 1004
AcoI YGGCCR 1 cut(s) 472
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 4 cut(s) 197, 465, 481, 501
AfiI CCNNNNNNNGG 2 cut(s) 57, 162
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 7 cut(s) 193, 347, 394, 627, 872, 911, 917
AhdI GACNNNNNGTC 1 cut(s) 248
AjnI CCWGG 3 cut(s) 56, 489, 807
AluBI AGCT 5 cut(s) 390, 421, 605, 769, 956
AluI AGCT 5 cut(s) 390, 421, 605, 769, 956
Alw26I GTCTC 2 cut(s) 766, 981
AlwI GGATC 2 cut(s) 658, 1004
AoxI GGCC 6 cut(s) 79, 93, 151, 199, 472, 992
ApeKI GCWGC 4 cut(s) 486, 521, 545, 605
AseI ATTAAT 1 cut(s) 659
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 7 cut(s) 79, 84, 93, 99, 152, 200, 992
AsuC2I CCSGG 3 cut(s) 77, 92, 97
AsuHPI GGTGA 2 cut(s) 152, 245
AvaII GGWCC 2 cut(s) 84, 99
BaeGI GKGCMC 1 cut(s) 789
BalI TGGCCA 1 cut(s) 474
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 9, 24
BbvI GCAGC 4 cut(s) 473, 508, 532, 592
BccI CCATC 3 cut(s) 103, 269, 400
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 3 cut(s) 58, 491, 809
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 3 cut(s) 77, 92, 97
BcoDI GTCTC 2 cut(s) 766, 981
BfaI CTAG 1 cut(s) 531
BfmI CTRYAG 1 cut(s) 554
BfoI RGCGCY 1 cut(s) 188
BisI GCNGC 4 cut(s) 487, 522, 546, 606
BlsI GCNGC 4 cut(s) 488, 523, 547, 607
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 6 cut(s) 58, 77, 92, 97, 491, 809
Bme18I GGWCC 2 cut(s) 84, 99
BmeRI GACNNNNNGTC 1 cut(s) 248
BmgT120I GGNCC 7 cut(s) 79, 84, 93, 99, 152, 200, 992
BmiI GGNNCC 9 cut(s) 55, 80, 100, 115, 136, 154, 202, 303, 561
BmrFI CCNGG 6 cut(s) 58, 77, 92, 97, 491, 809
BmsI GCATC 1 cut(s) 952
BpiI GAAGAC 2 cut(s) 9, 24
BplI GAGNNNNNCTC 2 cut(s) 171, 203
BpmI CTGGAG 1 cut(s) 546
BpuMI CCSGG 3 cut(s) 77, 92, 97
BsaJI CCNNGG 4 cut(s) 76, 178, 822, 1013
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 162
Bse118I RCCGGY 2 cut(s) 50, 81
Bse1I ACTGG 2 cut(s) 487, 563
Bse3DI GCAATG 1 cut(s) 894
BseBI CCWGG 3 cut(s) 58, 491, 809
BseDI CCNNGG 4 cut(s) 76, 178, 822, 1013
BseGI GGATG 2 cut(s) 736, 1012
BseLI CCNNNNNNNGG 2 cut(s) 57, 162
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 2 cut(s) 444, 761
BseNI ACTGG 2 cut(s) 487, 563
BseRI GAGGAG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 4 cut(s) 473, 508, 532, 592
BsgI GTGCAG 1 cut(s) 918
Bsh1236I CGCG 1 cut(s) 404
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 6 cut(s) 81, 95, 153, 201, 474, 994
BsiEI CGRYCG 1 cut(s) 91
BsiSI CCGG 8 cut(s) 51, 66, 76, 82, 91, 96, 138, 150
BslI CCNNNNNNNGG 2 cut(s) 57, 162
BsmAI GTCTC 2 cut(s) 766, 981
BsmBI CGTCTC 1 cut(s) 981
BsnI GGCC 6 cut(s) 81, 95, 153, 201, 474, 994
Bsp1286I GDGCHC 2 cut(s) 116, 789
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 3 cut(s) 415, 650, 1009
Bsp19I CCATGG 2 cut(s) 822, 1013
BspACI CCGC 3 cut(s) 48, 163, 536
BspANI GGCC 6 cut(s) 81, 95, 153, 201, 474, 994
BspCNI CTCAG 2 cut(s) 445, 762
BspFNI CGCG 1 cut(s) 404
BspHI TCATGA 1 cut(s) 949
BspLI GGNNCC 9 cut(s) 55, 80, 100, 115, 136, 154, 202, 303, 561
BspPI GGATC 2 cut(s) 658, 1004
BspQI GCTCTTC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 894
BsrFI RCCGGY 2 cut(s) 50, 81
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 2 cut(s) 487, 563
BssAI RCCGGY 2 cut(s) 50, 81
BssECI CCNNGG 4 cut(s) 76, 178, 822, 1013
BssMI GATC 3 cut(s) 415, 650, 1009
BssT1I CCWWGG 2 cut(s) 822, 1013
Bst2UI CCWGG 3 cut(s) 58, 491, 809
Bst6I CTCTTC 2 cut(s) 194, 597
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 2 cut(s) 453, 770
BstDSI CCRYGG 2 cut(s) 822, 1013
BstF5I GGATG 2 cut(s) 736, 1012
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 3 cut(s) 418, 653, 1012
BstMAI GTCTC 2 cut(s) 766, 981
BstMBI GATC 3 cut(s) 415, 650, 1009
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 4 cut(s) 387, 542, 680, 962
BstNI CCWGG 3 cut(s) 58, 491, 809
BstNSI RCATGY 2 cut(s) 528, 742
BstSCI CCNGG 6 cut(s) 56, 75, 90, 95, 489, 807
BstSFI CTRYAG 1 cut(s) 554
BstSLI GKGCMC 1 cut(s) 789
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 4 cut(s) 473, 508, 532, 592
BstV2I GAAGAC 2 cut(s) 9, 24
BstX2I RGATCY 1 cut(s) 650
BstXI CCANNNNNNTGG 1 cut(s) 482
BstYI RGATCY 1 cut(s) 650
BsuRI GGCC 6 cut(s) 81, 95, 153, 201, 474, 994
BtgI CCRYGG 2 cut(s) 822, 1013
BtgZI GCGATG 2 cut(s) 419, 980
BtsCI GGATG 2 cut(s) 736, 1012
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 1 cut(s) 526
CciI TCATGA 1 cut(s) 949
CfoI GCGC 1 cut(s) 187
Cfr10I RCCGGY 2 cut(s) 50, 81
Cfr13I GGNCC 7 cut(s) 79, 84, 93, 99, 152, 200, 992
CpoI CGGWCCG 1 cut(s) 84
CseI GACGC 1 cut(s) 410
Csp6I GTAC 4 cut(s) 196, 464, 480, 500
CspI CGGWCCG 1 cut(s) 84
CviAII CATG 9 cut(s) 355, 525, 631, 739, 790, 823, 920, 950, 1014
CviQI GTAC 4 cut(s) 196, 464, 480, 500
DdeI CTNAG 2 cut(s) 453, 770
DpnI GATC 3 cut(s) 417, 652, 1011
DpnII GATC 3 cut(s) 415, 650, 1009
DraI TTTAAA 1 cut(s) 226
DraIII CACNNNGTG 1 cut(s) 716
DriI GACNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 2 cut(s) 194, 597
Eam1105I GACNNNNNGTC 1 cut(s) 248
EarI CTCTTC 2 cut(s) 194, 597
EciI GGCGGA 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 822, 1013
Eco24I GRGCYC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 84, 99
EcoRII CCWGG 3 cut(s) 56, 489, 807
EcoT14I CCWWGG 2 cut(s) 822, 1013
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 2 cut(s) 822, 1013
Esp3I CGTCTC 1 cut(s) 981
FaeI CATG 9 cut(s) 358, 528, 634, 742, 793, 826, 923, 953, 1017
FatI CATG 9 cut(s) 354, 524, 630, 738, 789, 822, 919, 949, 1013
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 4 cut(s) 487, 522, 546, 606
FokI GGATG 2 cut(s) 743, 1019
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 4 cut(s) 487, 522, 546, 606
FspBI CTAG 1 cut(s) 531
GlaI GCGC 1 cut(s) 186
GluI GCNGC 4 cut(s) 487, 522, 546, 606
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 6 cut(s) 81, 95, 153, 201, 474, 994
HapII CCGG 8 cut(s) 51, 66, 76, 82, 91, 96, 138, 150
HgaI GACGC 1 cut(s) 410
HhaI GCGC 1 cut(s) 187
Hin1II CATG 9 cut(s) 358, 528, 634, 742, 793, 826, 923, 953, 1017
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HinfI GANTC 4 cut(s) 4, 119, 169, 449
HpaII CCGG 8 cut(s) 51, 66, 76, 82, 91, 96, 138, 150
HphI GGTGA 2 cut(s) 152, 245
Hpy166II GTNNAC 1 cut(s) 853
Hpy188I TCNGA 5 cut(s) 88, 118, 179, 454, 751
Hpy188III TCNNGA 4 cut(s) 106, 531, 596, 950
Hpy8I GTNNAC 1 cut(s) 853
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 1 cut(s) 635
HpyCH4V TGCA 6 cut(s) 221, 311, 524, 719, 882, 899
HpyF10VI GCNNNNNNNGC 4 cut(s) 387, 542, 680, 962
HpyF3I CTNAG 2 cut(s) 453, 770
Hsp92II CATG 9 cut(s) 358, 528, 634, 742, 793, 826, 923, 953, 1017
HspAI GCGC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 3 cut(s) 415, 650, 1009
LguI GCTCTTC 1 cut(s) 194
LmnI GCTCC 2 cut(s) 119, 565
Lsp1109I GCAGC 4 cut(s) 473, 508, 532, 592
LweI GCATC 1 cut(s) 952
MaeI CTAG 1 cut(s) 531
MaeIII GTNAC 3 cut(s) 140, 336, 644
MalI GATC 3 cut(s) 417, 652, 1011
MbiI CCGCTC 1 cut(s) 536
MboI GATC 3 cut(s) 415, 650, 1009
MboII GAAGA 8 cut(s) 9, 12, 24, 178, 181, 354, 458, 584
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 116, 789
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 5 cut(s) 210, 312, 656, 912, 1018
MluNI TGGCCA 1 cut(s) 474
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 111, 141
MnlI CCTC 5 cut(s) 19, 36, 173, 315, 448
Mox20I TGGCCA 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 474
MseI TTAA 3 cut(s) 225, 659, 981
Msp20I TGGCCA 1 cut(s) 474
MspA1I CMGCKG 3 cut(s) 421, 605, 769
MspI CCGG 8 cut(s) 51, 66, 76, 82, 91, 96, 138, 150
MspR9I CCNGG 6 cut(s) 58, 77, 92, 97, 491, 809
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 3 cut(s) 58, 491, 809
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 4 cut(s) 387, 542, 680, 962
NciI CCSGG 3 cut(s) 77, 92, 97
NcoI CCATGG 2 cut(s) 822, 1013
NdeII GATC 3 cut(s) 415, 650, 1009
NlaIII CATG 9 cut(s) 358, 528, 634, 742, 793, 826, 923, 953, 1017
NlaIV GGNNCC 9 cut(s) 55, 80, 100, 115, 136, 154, 202, 303, 561
NmuCI GTSAC 3 cut(s) 140, 336, 644
NspI RCATGY 2 cut(s) 528, 742
PaeI GCATGC 1 cut(s) 528
PagI TCATGA 1 cut(s) 949
PciI ACATGT 1 cut(s) 738
PciSI GCTCTTC 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 3 cut(s) 4, 169, 449
PkrI GCNGC 4 cut(s) 488, 523, 547, 607
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PscI ACATGT 1 cut(s) 738
PshBI ATTAAT 1 cut(s) 659
PsiI TTATAA 1 cut(s) 437
Psp6I CCWGG 3 cut(s) 56, 489, 807
PspGI CCWGG 3 cut(s) 56, 489, 807
PspN4I GGNNCC 9 cut(s) 55, 80, 100, 115, 136, 154, 202, 303, 561
PspPI GGNCC 7 cut(s) 79, 84, 93, 99, 152, 200, 992
PsuI RGATCY 1 cut(s) 650
PvuII CAGCTG 3 cut(s) 421, 605, 769
RsaI GTAC 4 cut(s) 197, 465, 481, 501
RsaNI GTAC 4 cut(s) 196, 464, 480, 500
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 194
SaqAI TTAA 3 cut(s) 225, 659, 981
SatI GCNGC 4 cut(s) 487, 522, 546, 606
Sau3AI GATC 3 cut(s) 415, 650, 1009
Sau96I GGNCC 7 cut(s) 79, 84, 93, 99, 152, 200, 992
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 6 cut(s) 58, 77, 92, 97, 491, 809
SduI GDGCHC 2 cut(s) 116, 789
SfaNI GCATC 1 cut(s) 952
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 2 cut(s) 84, 99
SphI GCATGC 1 cut(s) 528
Sse9I AATT 5 cut(s) 210, 312, 656, 912, 1018
SsiI CCGC 3 cut(s) 48, 163, 536
SspMI CTAG 1 cut(s) 531
StyD4I CCNGG 6 cut(s) 56, 75, 90, 95, 489, 807
StyI CCWWGG 2 cut(s) 822, 1013
TaqI TCGA 1 cut(s) 960
TaqII GACCGA 1 cut(s) 287
TasI AATT 5 cut(s) 210, 312, 656, 912, 1018
TatI WGTACW 3 cut(s) 463, 479, 499
TfiI GAWTC 3 cut(s) 4, 169, 449
Tru1I TTAA 3 cut(s) 225, 659, 981
Tru9I TTAA 3 cut(s) 225, 659, 981
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 3 cut(s) 140, 336, 644
TseI GCWGC 4 cut(s) 486, 521, 545, 605
Tsp45I GTSAC 3 cut(s) 140, 336, 644
TspDTI ATGAA 5 cut(s) 17, 354, 654, 938, 966
TspGWI ACGGA 3 cut(s) 18, 91, 663
TspRI CASTG 1 cut(s) 574
VpaK11BI GGWCC 2 cut(s) 84, 99
VspI ATTAAT 1 cut(s) 659
XbaI TCTAGA 1 cut(s) 530
XceI RCATGY 2 cut(s) 528, 742
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 1 cut(s) 531
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.