Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003981

Physical Location & Seq

Genomic Coordinates Reverse (-)

17975659 .. 17983708

Gene Structure 8050 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1201400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2973 bp

ATGCCTATAAGAAAGAAGGAGAGCTCGGAGCAACACCTTGTGATCAAACCCCACTTGCAGAACCCAGTGAACCCAGTTCAAAAGGCACCGAAAACTGCCCAAAATGGCAAAGGTCCACCACCCCAGGAACCCCAAAACCCCAAACTCCATAACCAAACATCACCTCCAGCAAAAAACAGAGGAAGAAGAAGGGGCAGAGGCGGTCGGAAGTCCGATCAAGGTGATGTTTGTATGCGGCCGAGCTCGCGGCACTGCGCTGTGGCTCATCTACCTGCCTCACCAAGCTTGGGTGGTGCTGCTCCTCTAGCTACTAGTCCAAATGGTTATGTGGAAAATGGTGGCAACTCGGTTTCCATGGAGATGGGGTTTCCGACTTCGAGCAAGTCGTTGAGTTTTGCTCCTCGGCCTGGTTATGGACAAGTTGGGATCAAGTGTATTGTGAAGGCCAACCATTTCTTTGCAGAGTTACCAGAGAAGGACTTGAACCATTATGATGTTAGCATAACTCCTGAAGTTGCATCGAGAGCTTTGAACAGAGCTATCATGGCAGAACTGGTGAGGTTGTACAAGGAATCTGACTTAGGAATGAGACTGCCTGCTTATGATGGTAGAAAGAGTTTGTACACTGCTGGTGAGCTGCCCTTTGCTTGGAAGGAGTTCAATATTAAGCTTGTTGATGAACCGGATGGAATCAGTGGCCGAAAAAGAGAAAGAGAATATAAAGTGGTAATTAAATTCGTTGCACGAGCAAACATGCATCATCTGGGCCAGTTTCTGGCTGGTAAGTGTGCTGATGCTCCACAGGAAGCGCTCCAAATTCTTGACATTGTATTAAGAGAGCTCTCAAACAAGAGGTACTGCCCCATTGGGAGATCCTTCTTTTCACCCAATATCAGAACACCACAACGGCTTGGTGAGGGGTTGGAATCATGGTGCGGGTTTTACCAAAGTATAAGACCTACCCAAATGGGGCTTTCCCTCAACATTGATATGGCTTCAGCTGCGTTTATTGAGCCTCTCCCTGTAATAGAGTTTGTAGCCCAGCTTCTAGGCAAAGATGTATTGTCTAGAACATTGTCTGATGCTGACCGTGTAAAGATTAAAAAGGCTCTTAGAGGTGTGAAAGTTGAAGTTACACACAGAGGGAGTGTTCGAAGAAAGTATCGAGTTTCAGGATTGACATCTCAACCTACAAGAGAACTAGTGTTTCCTGTTGATGAGAACTTGACCATGAAGTCCGTAATTGAATACTTCCAAGAGATGTATGGATTCACCATTCAACACGGGCATCTTCCTTGCCTTCAAGTTGGTAATCAGAAGAAGGCAAACTATTTACCCATGGAGGCCTGCAAAATTGTTGAGGGGCAACGGTATACAAAGAGGTTGAATGAGAAGCAAATTACTGCTCTCTTAAAGGTTACATGTCAAAGACCTAGGGATCGTGAAAATGACATCCTGCAGACTGTTCAACACAACGCATATGACCAAGATCCTTATGCGAAGGAGTTTGGAATCAAAATCAGTGAAAAACTAGCTTCAGTCGAAGCTCGAATTCTTCCTGCTCCTTGGCTGAAGTATCATGAAACTGGAAAAGAAAAGAATTGTTTGCCTCAAGTTGGTCAGTGGAATATGATGAACAAGAAAATGATTAATGGAATGACTGTCAGTCGGTGGGCTTGTATTAATTTTTCAAGGGGAGTGCAAGAGAGTGTCGCACGTGGATTTTGCAGTGAACTTGCTCAAATGTGTCAAGTATCTGGCATGGAATTTAATTCAGAACCTGTAATTCCAATCTACAATGCGAGGCCTGAACAAGTAGAGAAAGCTTTGAAGCATGTTTATCATGCGTCCATGAACAAGACCAAAGGAAAAGATTTAGAGCTCCTATTGGCCATTTTGCCTGACAACAACGGGTCCCTGTATGGTGATATCAAGCGAATATGTGAAACAGATCTCGGTTTAATATCACAATGCTGTCTCACAAAGTATGTTTTCAAGATCAGCAAACAGTACTTGGCTAATGTCTCCCTAAAGATAAATGTTAAGATGGGTGGCAGAAACACTGTTCTCTTGGATGCTATCAGCTGCAGGATACCTTTGGTCAGTGATATACCAACCATAATCTTTGGAGCAGATGTGACTCACCCTGAGAATGGAGAAGATTCCAGCCCCTCAATTGCTGCTGTGGTAGCTTCCCAGGACTGGCCCGAAGTGACCAAATATGCTGGATTAGTTTGTGCTCAAGCTCACAGACAGGAACTCATACAGGATTTGTATAAAACATGGCAAGACCCTGTTCGTGGCACAGTCAGTGGTGGCATGATCCGGGATCTTCTAGTTTCGTTTAGGAAGGCAACAGGACAGAAGCCCCTAAGGATTATATTTTACAGGGATGGTGTAAGTGAAGGTCAATTTTACCAAGTCTTACTTTACGAGCTTGATGCAATCCGGAAGGCATGCGCTTCTCTGGAACCCAACTATCAACCACCAGTGACTTTCATTGTGGTTCAAAAACGGCACCACACACGATTATTTGCTAATAACCATAGGGACAGGAGCAGCATAGACAAGAGTGGGAACATATTACCTGGTACTGTGGTTGATACTAAGATTTGCCATCCAACCGAACATGATTTCTATCTCTGCAGTCATGCTGGTATTCAGGGGACAAGTCGCCCAGCTCACTACCATGTTATTTGGGATGAGAATAATTTTACTGCTGATGGAATCCAATCATTGACAAATAACCTGTGTTACACATATGCAAGGTGCACGCGCTCTGTCTCTGTTGTACCTCCGGCATACTATGCGCATTTGGCTGCTTTCCGTGCTCGATTCTATCTGGAGCCAGATATGCAAGAGAATGGCTCTACTGGTCACACTGCTAAGGGTACACGCGCTGCTGGCGAGTCTGGAGTGCGACCATTACCAGCCTTGAAGGAGAATGTAAAGAGAGTAATGTTTTATTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0010586 GO:0016070 GO:0019827 GO:0032501 GO:0032502 GO:0034641 GO:0034660 GO:0035019 GO:0035198 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0046483 GO:0048509 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051704 GO:0051707 GO:0061980 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0098542 GO:0098727 GO:1901360 GO:1901363 GO:1902183

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

991

Amino Acids

110.76

Weight (kDa)

9.3

Isoelectric Point (pI)

45.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	144 - 278	8.5e-35	N-terminal domain of argonaute
ArgoL1	PF08699	288 - 338	5e-19	Argonaute linker 1 domain
PAZ	PF02170	362 - 470	2.4e-28	PAZ domain
ArgoL2	PF16488	480 - 526	1e-16	Argonaute linker 2 domain
ArgoMid	PF16487	537 - 614	1.8e-14	Mid domain of argonaute
Piwi	PF02171	629 - 947	1.9e-115	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43810 AT5G43810 AT5G43810 AT5G43810
fragaria_vesca	FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630
malus_domestica	MD13G1201400.v1.1 MD16G1201200.v1.1
prunus_persica	Prupe.1G022900_v2.0.a1 Prupe.1G022900_v2.0.a1
pyrus_communis	pycom13g17460 pycom16g16970
rosa_chinensis	RchiOBHm_Chr4g0388221 RchiOBHm_Chr4g0388231
rosa_laevigata	RLG00000010073
rosa_roxburghii	Rroxscaffold_5G00334710
rosa_rugosa	Rorug03G0316100 Rorug03G0316200 Rorug03G0316300 Rorug03G0316300 Rorug03G0316300 Rorug03G0316300 Rorug03G0316400
rosa_samantha	Rh4AG021700 Rh4BG015800 Rh4CG021900 Rh4DG016600
rosa_wichuraiana	Rw4G001420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1234
Acc16I	TGCGCA	1 cut(s)	2811
Acc36I	ACCTGC	1 cut(s)	280
AccB1I	GGYRCC	2 cut(s)	85, 2517
AccB7I	CCANNNNNTGG	1 cut(s)	775
AccI	GTMKAC	1 cut(s)	1373
AccII	CGCG	3 cut(s)	247, 2776, 2898
AccIII	TCCGGA	1 cut(s)	2448
AciI	CCGC	4 cut(s)	201, 235, 247, 936
AclWI	GGATC	6 cut(s)	434, 867, 1446, 1484, 2317, 2337
AcoI	YGGCCR	3 cut(s)	236, 697, 1890
AcsI	RAATTY	4 cut(s)	734, 816, 1551, 1766
AcuI	CTGAAG	4 cut(s)	531, 981, 1521, 1592
AcvI	CACGTG	1 cut(s)	1718
AdeI	CACNNNGTG	1 cut(s)	40
AfaI	GTAC	7 cut(s)	566, 623, 857, 2012, 2593, 2793, 2893
AfeI	AGCGCT	1 cut(s)	810
AflIII	ACRYGT	1 cut(s)	1421
AhdI	GACNNNNNGTC	1 cut(s)	1665
AhlI	ACTAGT	2 cut(s)	311, 1201
AjnI	CCWGG	4 cut(s)	123, 406, 2196, 2587
Alw21I	GWGCWC	7 cut(s)	26, 245, 843, 1884, 2242, 2774, 2833
Alw26I	GTCTC	4 cut(s)	583, 1982, 2029, 2788
Alw44I	GTGCAC	1 cut(s)	2770
AlwI	GGATC	6 cut(s)	434, 867, 1446, 1484, 2317, 2337
AlwNI	CAGNNNCTG	2 cut(s)	775, 1781
Aor13HI	TCCGGA	1 cut(s)	2448
Aor51HI	AGCGCT	1 cut(s)	810
AoxI	GGCC	9 cut(s)	236, 404, 444, 697, 766, 1344, 1805, 1890, 2204
ApaLI	GTGCAC	1 cut(s)	2770
ApeKI	GCWGC	8 cut(s)	296, 637, 1001, 2085, 2180, 2559, 2819, 2900
ApoI	RAATTY	4 cut(s)	734, 816, 1551, 1766
AseI	ATTAAT	2 cut(s)	1650, 1683
Asp700I	GAANNNNTTC	1 cut(s)	656
AspA2I	CCTAGG	1 cut(s)	1433
AspLEI	GCGC	6 cut(s)	257, 811, 2462, 2778, 2812, 2900
AspS9I	GGNCC	4 cut(s)	113, 766, 1914, 2205
AsuC2I	CCSGG	1 cut(s)	2327
AsuII	TTCGAA	1 cut(s)	1153
AvaII	GGWCC	2 cut(s)	113, 1914
AvrII	CCTAGG	1 cut(s)	1433
AxyI	CCTNAGG	1 cut(s)	2372
BaeGI	GKGCMC	1 cut(s)	2774
BaeI	ACNNNNGTAYC	2 cut(s)	2583, 2616
BalI	TGGCCA	1 cut(s)	1892
BanI	GGYRCC	2 cut(s)	85, 2517
BanII	GRGCYC	4 cut(s)	26, 245, 843, 1884
BarI	GAAGNNNNNNTAC	2 cut(s)	1029, 1061
BauI	CACGAG	1 cut(s)	744
BbrPI	CACGTG	1 cut(s)	1718
Bbv12I	GWGCWC	7 cut(s)	26, 245, 843, 1884, 2242, 2774, 2833
BbvI	GCAGC	8 cut(s)	283, 624, 988, 2072, 2167, 2571, 2806, 2887
BccI	CCATC	7 cut(s)	355, 599, 680, 2041, 2387, 2625, 2717
BceAI	ACGGC	2 cut(s)	923, 2531
BcgI	CGANNNNNNTGC	2 cut(s)	2423, 2457
BciT130I	CCWGG	4 cut(s)	125, 408, 2198, 2589
BciVI	GTATCC	1 cut(s)	2085
BclI	TGATCA	1 cut(s)	42
BcnI	CCSGG	1 cut(s)	2327
BcoDI	GTCTC	4 cut(s)	583, 1982, 2029, 2788
BcuI	ACTAGT	2 cut(s)	311, 1201
BfaI	CTAG	8 cut(s)	305, 312, 1049, 1068, 1202, 1434, 1532, 2336
BfmI	CTRYAG	3 cut(s)	1457, 2086, 2644
BfoI	RGCGCY	1 cut(s)	812
BfuAI	ACCTGC	1 cut(s)	280
BfuI	GTATCC	1 cut(s)	2085
BglII	AGATCT	1 cut(s)	1951
BlnI	CCTAGG	1 cut(s)	1433
BmcAI	AGTACT	1 cut(s)	2012
Bme1390I	CCNGG	5 cut(s)	125, 408, 2198, 2327, 2589
Bme18I	GGWCC	2 cut(s)	113, 1914
BmeRI	GACNNNNNGTC	1 cut(s)	1665
BmgT120I	GGNCC	4 cut(s)	113, 766, 1914, 2205
BmiI	GGNNCC	7 cut(s)	87, 129, 1915, 1916, 2472, 2519, 2847
BmrFI	CCNGG	5 cut(s)	125, 408, 2198, 2327, 2589
BmrI	ACTGGG	2 cut(s)	59, 68
BmsI	GCATC	7 cut(s)	527, 766, 784, 1072, 1297, 2065, 2431
BmuI	ACTGGG	2 cut(s)	59, 68
BplI	GAGNNNNNCTC	4 cut(s)	382, 414, 2852, 2884
BpmI	CTGGAG	3 cut(s)	150, 2864, 2934
Bpu10I	CCTNAGC	1 cut(s)	2886
Bpu14I	TTCGAA	1 cut(s)	1153
BpuEI	CTTGAG	2 cut(s)	1596, 2226
BpuMI	CCSGG	1 cut(s)	2327
BsaAI	YACGTR	1 cut(s)	1718
BsaBI	GATNNNNATC	2 cut(s)	1180, 2637
BsaJI	CCNNGG	7 cut(s)	123, 354, 401, 1338, 1433, 1565, 2196
BsaWI	WCCGGW	2 cut(s)	682, 2448
BsaXI	ACNNNNNCTCC	6 cut(s)	148, 178, 350, 380, 2121, 2151
Bse1I	ACTGG	8 cut(s)	65, 74, 558, 769, 1591, 2207, 2489, 2878
Bse21I	CCTNAGG	1 cut(s)	2372
Bse8I	GATNNNNATC	2 cut(s)	1180, 2637
BseAI	TCCGGA	1 cut(s)	2448
BseBI	CCWGG	4 cut(s)	125, 408, 2198, 2589
BseDI	CCNNGG	7 cut(s)	123, 354, 401, 1338, 1433, 1565, 2196
BseGI	GGATG	6 cut(s)	691, 1452, 2080, 2398, 2617, 2707
BseJI	GATNNNNATC	2 cut(s)	1180, 2637
BseMII	CTCAG	1 cut(s)	2139
BseNI	ACTGG	8 cut(s)	65, 74, 558, 769, 1591, 2207, 2489, 2878
BseRI	GAGGAG	2 cut(s)	291, 390
BseSI	GKGCMC	1 cut(s)	2774
BseX3I	CGGCCG	1 cut(s)	236
BseXI	GCAGC	8 cut(s)	283, 624, 988, 2072, 2167, 2571, 2806, 2887
BseYI	CCCAGC	2 cut(s)	1041, 2677
Bsh1236I	CGCG	3 cut(s)	247, 2776, 2898
Bsh1285I	CGRYCG	2 cut(s)	205, 239
BshFI	GGCC	9 cut(s)	238, 406, 446, 699, 768, 1346, 1807, 1892, 2206
BshNI	GGYRCC	2 cut(s)	85, 2517
BsiEI	CGRYCG	2 cut(s)	205, 239
BsiHKAI	GWGCWC	7 cut(s)	26, 245, 843, 1884, 2242, 2774, 2833
BsiSI	CCGG	4 cut(s)	683, 2326, 2449, 2798
BslFI	GGGAC	3 cut(s)	1900, 2564, 2680
BsmAI	GTCTC	4 cut(s)	583, 1982, 2029, 2788
BsmFI	GGGAC	3 cut(s)	1900, 2564, 2680
BsnI	GGCC	9 cut(s)	238, 406, 446, 699, 768, 1346, 1807, 1892, 2206
Bsp119I	TTCGAA	1 cut(s)	1153
Bsp1286I	GDGCHC	7 cut(s)	26, 245, 843, 1884, 2242, 2774, 2833
Bsp13I	TCCGGA	1 cut(s)	2448
Bsp1407I	TGTACA	2 cut(s)	564, 621
Bsp19I	CCATGG	2 cut(s)	354, 1338
BspACI	CCGC	4 cut(s)	201, 235, 247, 936
BspANI	GGCC	9 cut(s)	238, 406, 446, 699, 768, 1346, 1807, 1892, 2206
BspCNI	CTCAG	1 cut(s)	2140
BspEI	TCCGGA	1 cut(s)	2448
BspFNI	CGCG	3 cut(s)	247, 2776, 2898
BspHI	TCATGA	1 cut(s)	1579
BspLI	GGNNCC	7 cut(s)	87, 129, 1915, 1916, 2472, 2519, 2847
BspMAI	CTGCAG	3 cut(s)	1461, 2090, 2648
BspMI	ACCTGC	1 cut(s)	280
BspPI	GGATC	6 cut(s)	434, 867, 1446, 1484, 2317, 2337
BspT104I	TTCGAA	1 cut(s)	1153
BspT107I	GGYRCC	2 cut(s)	85, 2517
BsrGI	TGTACA	2 cut(s)	564, 621
BsrI	ACTGG	8 cut(s)	65, 74, 558, 769, 1591, 2207, 2489, 2878
BssECI	CCNNGG	7 cut(s)	123, 354, 401, 1338, 1433, 1565, 2196
BssNAI	GTATAC	1 cut(s)	1374
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	4 cut(s)	354, 1338, 1433, 1565
Bst1107I	GTATAC	1 cut(s)	1374
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	4 cut(s)	125, 408, 2198, 2589
Bst4CI	ACNGT	8 cut(s)	1091, 1371, 1465, 1663, 2010, 2065, 2308, 2596
BstAPI	GCANNNNNTGC	1 cut(s)	2807
BstAUI	TGTACA	2 cut(s)	564, 621
BstBAI	YACGTR	1 cut(s)	1718
BstBI	TTCGAA	1 cut(s)	1153
BstC8I	GCNNGC	6 cut(s)	245, 597, 1348, 2458, 2774, 2905
BstDEI	CTNAG	6 cut(s)	580, 1112, 2148, 2372, 2607, 2886
BstDSI	CCRYGG	2 cut(s)	354, 1338
BstF5I	GGATG	6 cut(s)	691, 1452, 2080, 2398, 2617, 2707
BstFNI	CGCG	3 cut(s)	247, 2776, 2898
BstH2I	RGCGCY	1 cut(s)	812
BstHHI	GCGC	6 cut(s)	257, 811, 2462, 2778, 2812, 2900
BstMAI	GTCTC	4 cut(s)	583, 1982, 2029, 2788
BstMCI	CGRYCG	2 cut(s)	205, 239
BstNI	CCWGG	4 cut(s)	125, 408, 2198, 2589
BstNSI	RCATGY	4 cut(s)	757, 1425, 1838, 2460
BstSCI	CCNGG	5 cut(s)	123, 406, 2196, 2325, 2587
BstSFI	CTRYAG	3 cut(s)	1457, 2086, 2644
BstSLI	GKGCMC	1 cut(s)	2774
BstUI	CGCG	3 cut(s)	247, 2776, 2898
BstV1I	GCAGC	8 cut(s)	283, 624, 988, 2072, 2167, 2571, 2806, 2887
BstX2I	RGATCY	4 cut(s)	872, 1489, 1951, 2329
BstXI	CCANNNNNNTGG	1 cut(s)	361
BstYI	RGATCY	4 cut(s)	872, 1489, 1951, 2329
BstZ17I	GTATAC	1 cut(s)	1374
BstZI	CGGCCG	1 cut(s)	236
Bsu36I	CCTNAGG	1 cut(s)	2372
BsuI	GTATCC	1 cut(s)	2085
BsuRI	GGCC	9 cut(s)	238, 406, 446, 699, 768, 1346, 1807, 1892, 2206
BtgI	CCRYGG	2 cut(s)	354, 1338
BtsCI	GGATG	6 cut(s)	691, 1452, 2080, 2398, 2617, 2707
BtsI	GCAGTG	4 cut(s)	250, 624, 1735, 2880
BveI	ACCTGC	1 cut(s)	280
Cac8I	GCNNGC	6 cut(s)	245, 597, 1348, 2458, 2774, 2905
CaiI	CAGNNNCTG	2 cut(s)	775, 1781
CciI	TCATGA	1 cut(s)	1579
CfoI	GCGC	6 cut(s)	257, 811, 2462, 2778, 2812, 2900
Cfr13I	GGNCC	4 cut(s)	113, 766, 1914, 2205
CseI	GACGC	1 cut(s)	1836
CsiI	ACCWGGT	1 cut(s)	2587
Csp6I	GTAC	7 cut(s)	565, 622, 856, 2011, 2592, 2792, 2892
CviQI	GTAC	7 cut(s)	565, 622, 856, 2011, 2592, 2792, 2892
DdeI	CTNAG	6 cut(s)	580, 1112, 2148, 2372, 2607, 2886
DraIII	CACNNNGTG	1 cut(s)	40
DrdI	GACNNNNNNGTC	1 cut(s)	1234
DriI	GACNNNNNGTC	1 cut(s)	1665
DseDI	GACNNNNNNGTC	1 cut(s)	1234
EaeI	YGGCCR	3 cut(s)	236, 697, 1890
EagI	CGGCCG	1 cut(s)	236
Eam1105I	GACNNNNNGTC	1 cut(s)	1665
Ecl136II	GAGCTC	4 cut(s)	24, 243, 841, 1882
EclXI	CGGCCG	1 cut(s)	236
Eco130I	CCWWGG	4 cut(s)	354, 1338, 1433, 1565
Eco147I	AGGCCT	2 cut(s)	1346, 1807
Eco24I	GRGCYC	4 cut(s)	26, 245, 843, 1884
Eco32I	GATATC	1 cut(s)	1930
Eco47I	GGWCC	2 cut(s)	113, 1914
Eco47III	AGCGCT	1 cut(s)	810
Eco52I	CGGCCG	1 cut(s)	236
Eco53kI	GAGCTC	4 cut(s)	24, 243, 841, 1882
Eco57I	CTGAAG	4 cut(s)	531, 981, 1521, 1592
Eco72I	CACGTG	1 cut(s)	1718
Eco81I	CCTNAGG	1 cut(s)	2372
EcoICRI	GAGCTC	4 cut(s)	24, 243, 841, 1882
EcoO109I	RGGNCCY	1 cut(s)	1914
EcoRI	GAATTC	1 cut(s)	1551
EcoRII	CCWGG	4 cut(s)	123, 406, 2196, 2587
EcoRV	GATATC	1 cut(s)	1930
EcoT14I	CCWWGG	4 cut(s)	354, 1338, 1433, 1565
EcoT22I	ATGCAT	1 cut(s)	759
EcoT38I	GRGCYC	4 cut(s)	26, 245, 843, 1884
ErhI	CCWWGG	4 cut(s)	354, 1338, 1433, 1565
FalI	AAGNNNNNCTT	2 cut(s)	2412, 2444
FaqI	GGGAC	3 cut(s)	1900, 2564, 2680
FauI	CCCGC	1 cut(s)	929
FauNDI	CATATG	2 cut(s)	1480, 2761
FbaI	TGATCA	1 cut(s)	42
FblI	GTMKAC	1 cut(s)	1373
FokI	GGATG	6 cut(s)	698, 1439, 2087, 2405, 2604, 2714
FriOI	GRGCYC	4 cut(s)	26, 245, 843, 1884
FspAI	RTGCGCAY	1 cut(s)	2811
FspBI	CTAG	8 cut(s)	305, 312, 1049, 1068, 1202, 1434, 1532, 2336
FspI	TGCGCA	1 cut(s)	2811
GlaI	GCGC	6 cut(s)	256, 810, 2461, 2777, 2811, 2899
GsaI	CCCAGC	2 cut(s)	1045, 2681
GsuI	CTGGAG	3 cut(s)	150, 2864, 2934
HaeII	RGCGCY	1 cut(s)	812
HaeIII	GGCC	9 cut(s)	238, 406, 446, 699, 768, 1346, 1807, 1892, 2206
HapII	CCGG	4 cut(s)	683, 2326, 2449, 2798
HgaI	GACGC	1 cut(s)	1836
HhaI	GCGC	6 cut(s)	257, 811, 2462, 2778, 2812, 2900
Hin6I	GCGC	6 cut(s)	255, 809, 2460, 2776, 2810, 2898
HinP1I	GCGC	6 cut(s)	255, 809, 2460, 2776, 2810, 2898
HindIII	AAGCTT	3 cut(s)	283, 668, 1824
HpaII	CCGG	4 cut(s)	683, 2326, 2449, 2798
Hpy166II	GTNNAC	7 cut(s)	70, 116, 624, 1374, 1733, 2772, 2894
Hpy188I	TCNGA	9 cut(s)	28, 207, 214, 372, 577, 896, 1081, 1317, 1777
Hpy8I	GTNNAC	7 cut(s)	70, 116, 624, 1374, 1733, 2772, 2894
HpyCH4III	ACNGT	8 cut(s)	1091, 1371, 1465, 1663, 2010, 2065, 2308, 2596
HpyCH4IV	ACGT	1 cut(s)	1717
HpyF3I	CTNAG	6 cut(s)	580, 1112, 2148, 2372, 2607, 2886
HpySE526I	ACGT	1 cut(s)	1717
HspAI	GCGC	6 cut(s)	255, 809, 2460, 2776, 2810, 2898
KflI	GGGWCCC	1 cut(s)	1914
Kpn2I	TCCGGA	1 cut(s)	2448
Ksp22I	TGATCA	1 cut(s)	42
Lsp1109I	GCAGC	8 cut(s)	283, 624, 988, 2072, 2167, 2571, 2806, 2887
LweI	GCATC	7 cut(s)	527, 766, 784, 1072, 1297, 2065, 2431
MabI	ACCWGGT	1 cut(s)	2587
MaeI	CTAG	8 cut(s)	305, 312, 1049, 1068, 1202, 1434, 1532, 2336
MaeII	ACGT	1 cut(s)	1717
MaeIII	GTNAC	8 cut(s)	465, 1132, 1417, 2137, 2212, 2491, 2753, 2876
MboII	GAAGA	8 cut(s)	195, 198, 1167, 1283, 1330, 1547, 2171, 2324
MfeI	CAATTG	1 cut(s)	2175
MflI	RGATCY	4 cut(s)	872, 1489, 1951, 2329
MhlI	GDGCHC	7 cut(s)	26, 245, 843, 1884, 2242, 2774, 2833
MlsI	TGGCCA	1 cut(s)	1892
MluNI	TGGCCA	1 cut(s)	1892
MlyI	GAGTC	2 cut(s)	2134, 2918
MmeI	TCCRAC	4 cut(s)	185, 395, 903, 2645
Mox20I	TGGCCA	1 cut(s)	1892
Mph1103I	ATGCAT	1 cut(s)	759
MroI	TCCGGA	1 cut(s)	2448
MroXI	GAANNNNTTC	1 cut(s)	656
MscI	TGGCCA	1 cut(s)	1892
MslI	CAYNNNNRTG	4 cut(s)	359, 492, 989, 2688
Msp20I	TGGCCA	1 cut(s)	1892
MspA1I	CMGCKG	2 cut(s)	1001, 2085
MspI	CCGG	4 cut(s)	683, 2326, 2449, 2798
MspR9I	CCNGG	5 cut(s)	125, 408, 2198, 2327, 2589
MunI	CAATTG	1 cut(s)	2175
MvaI	CCWGG	4 cut(s)	125, 408, 2198, 2589
MvnI	CGCG	3 cut(s)	247, 2776, 2898
NciI	CCSGG	1 cut(s)	2327
NcoI	CCATGG	2 cut(s)	354, 1338
NdeI	CATATG	2 cut(s)	1480, 2761
NlaIV	GGNNCC	7 cut(s)	87, 129, 1915, 1916, 2472, 2519, 2847
NmeAIII	GCCGAG	2 cut(s)	264, 382
NmuCI	GTSAC	4 cut(s)	2137, 2212, 2491, 2876
NsbI	TGCGCA	1 cut(s)	2811
NsiI	ATGCAT	1 cut(s)	759
NspI	RCATGY	4 cut(s)	757, 1425, 1838, 2460
NspV	TTCGAA	1 cut(s)	1153
PaeI	GCATGC	1 cut(s)	2460
PagI	TCATGA	1 cut(s)	1579
PceI	AGGCCT	2 cut(s)	1346, 1807
PciI	ACATGT	1 cut(s)	1421
PcsI	WCGNNNNNNNCGW	1 cut(s)	383
PdmI	GAANNNNTTC	1 cut(s)	656
PfeI	GAWTC	8 cut(s)	572, 690, 926, 1269, 1512, 2162, 2727, 2835
PflMI	CCANNNNNTGG	1 cut(s)	775
PfoI	TCCNGGA	1 cut(s)	2325
PleI	GAGTC	2 cut(s)	2134, 2917
PmaCI	CACGTG	1 cut(s)	1718
PmlI	CACGTG	1 cut(s)	1718
PpsI	GAGTC	2 cut(s)	2134, 2917
Ppu21I	YACGTR	1 cut(s)	1718
PpuMI	RGGWCCY	1 cut(s)	1914
PscI	ACATGT	1 cut(s)	1421
PshBI	ATTAAT	2 cut(s)	1650, 1683
Psp124BI	GAGCTC	4 cut(s)	26, 245, 843, 1884
Psp5II	RGGWCCY	1 cut(s)	1914
Psp6I	CCWGG	4 cut(s)	123, 406, 2196, 2587
PspCI	CACGTG	1 cut(s)	1718
PspFI	CCCAGC	2 cut(s)	1041, 2677
PspGI	CCWGG	4 cut(s)	123, 406, 2196, 2587
PspN4I	GGNNCC	7 cut(s)	87, 129, 1915, 1916, 2472, 2519, 2847
PspPI	GGNCC	4 cut(s)	113, 766, 1914, 2205
PspPPI	RGGWCCY	1 cut(s)	1914
PstI	CTGCAG	3 cut(s)	1461, 2090, 2648
PstNI	CAGNNNCTG	2 cut(s)	775, 1781
PsuI	RGATCY	4 cut(s)	872, 1489, 1951, 2329
PvuII	CAGCTG	2 cut(s)	1001, 2085
RsaI	GTAC	7 cut(s)	566, 623, 857, 2012, 2593, 2793, 2893
RsaNI	GTAC	7 cut(s)	565, 622, 856, 2011, 2592, 2792, 2892
RseI	CAYNNNNRTG	4 cut(s)	359, 492, 989, 2688
SacI	GAGCTC	4 cut(s)	26, 245, 843, 1884
Sau96I	GGNCC	4 cut(s)	113, 766, 1914, 2205
ScaI	AGTACT	1 cut(s)	2012
SchI	GAGTC	2 cut(s)	2134, 2918
ScrFI	CCNGG	5 cut(s)	125, 408, 2198, 2327, 2589
SduI	GDGCHC	7 cut(s)	26, 245, 843, 1884, 2242, 2774, 2833
SexAI	ACCWGGT	1 cut(s)	2587
SfaNI	GCATC	7 cut(s)	527, 766, 784, 1072, 1297, 2065, 2431
SfcI	CTRYAG	3 cut(s)	1457, 2086, 2644
SfuI	TTCGAA	1 cut(s)	1153
SinI	GGWCC	2 cut(s)	113, 1914
SmiMI	CAYNNNNRTG	4 cut(s)	359, 492, 989, 2688
SmlI	CTYRAG	2 cut(s)	1611, 2241
SmoI	CTYRAG	2 cut(s)	1611, 2241
SpeI	ACTAGT	2 cut(s)	311, 1201
SphI	GCATGC	1 cut(s)	2460
SseBI	AGGCCT	2 cut(s)	1346, 1807
SsiI	CCGC	4 cut(s)	201, 235, 247, 936
SspI	AATATT	1 cut(s)	664
SspMI	CTAG	8 cut(s)	305, 312, 1049, 1068, 1202, 1434, 1532, 2336
SstI	GAGCTC	4 cut(s)	26, 245, 843, 1884
StuI	AGGCCT	2 cut(s)	1346, 1807
StyD4I	CCNGG	5 cut(s)	123, 406, 2196, 2325, 2587
StyI	CCWWGG	4 cut(s)	354, 1338, 1433, 1565
TaaI	ACNGT	8 cut(s)	1091, 1371, 1465, 1663, 2010, 2065, 2308, 2596
TaiI	ACGT	1 cut(s)	1720
TaqI	TCGA	7 cut(s)	377, 521, 1153, 1165, 1542, 1549, 2833
TatI	WGTACW	3 cut(s)	564, 621, 2010
TauI	GCSGC	2 cut(s)	238, 250
TfiI	GAWTC	8 cut(s)	572, 690, 926, 1269, 1512, 2162, 2727, 2835
TseFI	GTSAC	4 cut(s)	2137, 2212, 2491, 2876
TseI	GCWGC	8 cut(s)	296, 637, 1001, 2085, 2180, 2559, 2819, 2900
Tsp45I	GTSAC	4 cut(s)	2137, 2212, 2491, 2876
TspDTI	ATGAA	6 cut(s)	693, 1247, 1596, 1649, 1868, 2488
TspGWI	ACGGA	2 cut(s)	1228, 2816
Van91I	CCANNNNNTGG	1 cut(s)	775
VneI	GTGCAC	1 cut(s)	2770
VpaK11BI	GGWCC	2 cut(s)	113, 1914
VspI	ATTAAT	2 cut(s)	1650, 1683
XapI	RAATTY	4 cut(s)	734, 816, 1551, 1766
XbaI	TCTAGA	1 cut(s)	1067
XceI	RCATGY	4 cut(s)	757, 1425, 1838, 2460
XcmI	CCANNNNNNNNNTGG	2 cut(s)	776, 1262
XmaJI	CCTAGG	1 cut(s)	1433
XmiI	GTMKAC	1 cut(s)	1373
XmnI	GAANNNNTTC	1 cut(s)	656
XspI	CTAG	8 cut(s)	305, 312, 1049, 1068, 1202, 1434, 1532, 2336
ZrmI	AGTACT	1 cut(s)	2012
Zsp2I	ATGCAT	1 cut(s)	759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.