Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003981

Physical Location & Seq

Genomic Coordinates Forward (+)

62729134 .. 62735432

Gene Structure 6299 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010073

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2967 bp

ATGAAGGAGAGCTCAGAGCAACACCTTGTGATCAAAACCCATTTACAGAACACAGCAAACCCAGTTCAGAAGGCACCCAAGACTGGCCAAAATGGCAAAGGCCCACCAACCCCAGAACCTCAAAACCCCAAAACTCAGAACCAAACTTCACCTCCAACTAAGAACAGAGGAAGGAGAAGGGGGAGAGGTGGTCGGAAGTCTGATCAAGGTGATGTTTTTATGCGGCCAAGTTCAAGGCACTGCACGGTGGCTCATATTCCAGCCTCACCAAACTTGGCTGGAGGAGGTGCTTTAGTTAGTAGTACTACAAATGAGTCATTGGAAAATGGTGGAAATTCGTGTGCCATGGAGATGGGTTTTCCCACTTCCAGCAAGTCATTGAGCTTTGCACCTAGGCCTGGCTATGGACAAGCTGGGATAAAGTGTGTTGTAAAGGCCAACCATTTCTTCGCAGAGTTACCAGACAAGGACTTGAACCATTATGATGTTTGCATTACCCCCGAAGTTACATCGAGAGTTGTGAACAGAGCTATCATGGCTGAACTGGTGAGGTTGTACAGAGAATCTGACCTAGGAATGAGACTGCCCGCTTATGATGGTAGAAAGAGTCTGTATACTGCTGGTGAGCTACCCTTTGTTTGGAAGGAGTTTAACATCAAACTTACTGATGAAGAAGAACGAATCGATGGCCGAAAAAGAGAAAGGGAATATAAAGTGGCGATTAAATTTGTTGCTCGAGCGAACATGTATCATTTGAGCCAGTTTCTAGCCGGTAAATGTGCTGATGCTCCCCAGGAGGCCCTCCAAATTCTTGACATTGTTTTAAGAGAGCTCTCATCAAAAAGGTACTGTCCCATCGGGAGATCCTTCTTTTCACCTAATATCCGAACACCACAACGGCTTGGTGAGGGGTTGGAGTCATGGTGTGGGTTTTACCAAAGTATAAGGCCTACCCAAATGGGTCTTTCCCTCAATATTGATATGGCTTCAGCTGCATTTATTGAGCCTCTTCCTGTAATAGAGTTTGTAGCCCAGCTTCTGGGAAAAGATGTACTATCTAGGACATTGTCTGATGCTGACCGTGTAAAGATTAAGAAGGCTCTTAGAGGGGTGAAAGTTGAAGTTACCCACAGAGGGAGTGTTCGAAGAAAGTATCGAGTATCAGGACTGACATCTCAACCTACAAGAGAGCTTGTGTTTCCTGTTGATGAGAACTCAACGATGAAGTCAGTAATTGAATACTTCCAGGAGATGTATGGATTCACAATTCAACAAGGACATCTTCCTTGCCTTCAAGTTGGCGGAAATCAGAAGAAGGCAAATTATTTACCCATGGAGGCCTGCAAAATTGTTGAGGGGCAACGATATACAAAAAGGTTGAATGAAAAACAAATTACTGCTCTCTTGAAAGTTACATGTCAAAGGCCTAGGGATCGTGAAAATGACATCCTCCAGACCGTTCAACAGAATGCTTATGACCAAGACCCTTATGCTAATGAGTTTGGGATCAAAATCAGTGAAAAGCTTGCTTCAGTTGAGGCTCGAATTCTTCCAGCCCCTTGGCTGAAATATCATGAAACAGGAAAAGAAAAGAATTGTTTGCCTCAAGTAGGTCAGTGGAATATGATGAACAAGAAAATGATCAATGGAATGACAGTTAGCCGGTGGGCTTGTATTAACTTCTCACGGAGTGTGCAGGAGAGTGTTGCTCGTGGGTTTTGTAGTGAACTTGGTCAGATGTGTCAAGTCTCTGGCATGGAATTTAATCCAGAACCTGTAATTCCAATCTACAATGCCAGGCCTGAGCAAGTAGAGAAAGCTTTGAAACATGTATATCATGCATCCATGAACAAAACAAAAGGAAAAGAATTAGAGCTCTTATTGGCTATTTTGCCCGACAACAACGGGTCCCTGTATGGTGATATCAAGCGAATATGTGAAACAGATCTTGGATTAATCTCGCAATGCTGTCTTACAAAGCATGTTTTCAAGATTAGCAAACAGTATTTGGCTAATGTGTCTCTTAAGATAAATGTTAAGATGGGTGGCAGAAACACTGTTCTTTTGGATGCTATTAGCTGCAGGATACCTTTAGTCAGTGATATACCTACCATAATTTTCGGAGCAGACGTGACTCACCCGGAGAATGGAGAAGATACCAGTCCCTCGATTGCTGCGGTAGTGGCTTCCCAGGATTGGCCGGAAGTGACCAAATATGCTGGGTTAGTTTGTGCTCAAGCTCACAGACAGGAACTGATACAAGATTTGTACAAGACATGGCAGGACCCTGTTCGTGGCACAGTCAGTGGTGGTATGATCCGGGATCTTCTGGTTTCCTTTAGGAAAGCAACAGGGCAGAAGCCTCTCAGGATTATATTTTACAGGGATGGCGTAAGTGAAGGGCAATTTTATCAGGTCTTACTTTATGAGCTTGATGCGATTAGGAAGGCGTGTGCTTCTCTGGAACCCAACTATCAACCGCCAGTGACTTTCATTGTAGTACAGAAGCGGCACCACACACGATTATTTGCTAATAACCATAGGGACAGAAGCAGTGTAGACAAGAGTGGCAACATATTGCCTGGCACTGTAGTTGATACCAAGATATGCCATCCTACAGAATTTGATTTCTATCTCTGCAGCCATGCTGGTATTCAGGGGACAAGTCGGCCAGCTCACTACCATGTTCTCTGGGATGAGAATAATTTTACCCCTGATGGAATCCAGTCATTGACAAACAATCTTTGCTACACATATGCAAGGTGCACGCGCTCTGTGTCTGTTGTACCTCCGGCATATTATGCGCATTTGGCTGCTTTTCGTGCCCGATTCTATATGGAGCCAGATCTGCAGGATAATAGCTCTATTGGTCATGCTGCTAAGGGCACACGGGCTGCAGGAGAGTCTGGAGTCCGGCCGTTGCCAGCTTTAAAGGAGAATGTGAAGAGAGTAATGTTTTATTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0010586 GO:0016070 GO:0019827 GO:0032501 GO:0032502 GO:0034641 GO:0034660 GO:0035019 GO:0035198 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0046483 GO:0048509 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051704 GO:0051707 GO:0061980 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0098542 GO:0098727 GO:1901360 GO:1901363 GO:1902183

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

989

Amino Acids

110.68

Weight (kDa)

9.18

Isoelectric Point (pI)

46.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	141 - 275	2e-34	N-terminal domain of argonaute
ArgoL1	PF08699	285 - 335	5e-19	Argonaute linker 1 domain
PAZ	PF02170	359 - 468	7.8e-28	PAZ domain
ArgoL2	PF16488	478 - 524	9.9e-17	Argonaute linker 2 domain
ArgoMid	PF16487	535 - 612	1.7e-14	Mid domain of argonaute
Piwi	PF02171	627 - 946	1.6e-116	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43810 AT5G43810 AT5G43810 AT5G43810
fragaria_vesca	FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630
malus_domestica	MD13G1201400.v1.1 MD16G1201200.v1.1
prunus_persica	Prupe.1G022900_v2.0.a1 Prupe.1G022900_v2.0.a1
pyrus_communis	pycom13g17460 pycom16g16970
rosa_chinensis	RchiOBHm_Chr4g0388221 RchiOBHm_Chr4g0388231
rosa_laevigata	RLG00000010073
rosa_roxburghii	Rroxscaffold_5G00334710
rosa_rugosa	Rorug03G0316100 Rorug03G0316200 Rorug03G0316300 Rorug03G0316300 Rorug03G0316300 Rorug03G0316300 Rorug03G0316400
rosa_samantha	Rh4AG021700 Rh4BG015800 Rh4CG021900 Rh4DG016600
rosa_wichuraiana	Rw4G001420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2805
AccB1I	GGYRCC	2 cut(s)	73, 2511
AccB7I	CCANNNNNTGG	1 cut(s)	1039
AccI	GTMKAC	2 cut(s)	614, 2559
AccII	CGCG	1 cut(s)	2770
AciI	CCGC	6 cut(s)	223, 588, 1302, 2177, 2480, 2509
AclWI	GGATC	5 cut(s)	858, 1440, 1514, 2311, 2331
AcoI	YGGCCR	6 cut(s)	85, 224, 688, 2198, 2669, 2915
AcsI	RAATTY	6 cut(s)	334, 725, 807, 1545, 1760, 2621
AcuI	CTGAAG	2 cut(s)	972, 1515
AdeI	CACNNNGTG	2 cut(s)	28, 1691
AfaI	GTAC	7 cut(s)	304, 557, 848, 1053, 2270, 2502, 2787
AflII	CTTAAG	1 cut(s)	2024
AflIII	ACRYGT	3 cut(s)	744, 1415, 1828
AjiI	CACGTC	1 cut(s)	2131
AjnI	CCWGG	6 cut(s)	397, 792, 1245, 1796, 2190, 2581
AjuI	GAANNNNNNNTTGG	4 cut(s)	431, 463, 1932, 1964
Alw21I	GWGCWC	5 cut(s)	14, 834, 1878, 2236, 2768
Alw26I	GTCTC	3 cut(s)	574, 1753, 2025
Alw44I	GTGCAC	1 cut(s)	2764
AlwI	GGATC	5 cut(s)	858, 1440, 1514, 2311, 2331
AlwNI	CAGNNNCTG	3 cut(s)	1039, 1775, 2254
Ama87I	CYCGRG	1 cut(s)	735
ApaLI	GTGCAC	1 cut(s)	2764
ApeKI	GCWGC	7 cut(s)	992, 2079, 2174, 2640, 2813, 2876, 2894
ApoI	RAATTY	6 cut(s)	334, 725, 807, 1545, 1760, 2621
AseI	ATTAAT	1 cut(s)	1955
AspA2I	CCTAGG	3 cut(s)	392, 571, 1427
AspLEI	GCGC	2 cut(s)	2772, 2806
AspS9I	GGNCC	4 cut(s)	101, 799, 1908, 2284
AsuC2I	CCSGG	2 cut(s)	2141, 2321
AsuII	TTCGAA	1 cut(s)	1144
AvaI	CYCGRG	1 cut(s)	735
AvaII	GGWCC	2 cut(s)	1908, 2284
AvrII	CCTAGG	3 cut(s)	392, 571, 1427
BaeGI	GKGCMC	3 cut(s)	2768, 2827, 2888
BalI	TGGCCA	1 cut(s)	87
BanI	GGYRCC	2 cut(s)	73, 2511
BanII	GRGCYC	3 cut(s)	14, 834, 1878
BarI	GAAGNNNNNNTAC	2 cut(s)	1020, 1052
BauI	CACGAG	1 cut(s)	1710
Bbv12I	GWGCWC	5 cut(s)	14, 834, 1878, 2236, 2768
BbvI	GCAGC	7 cut(s)	979, 2066, 2161, 2652, 2800, 2863, 2881
BccI	CCATC	8 cut(s)	346, 590, 680, 863, 2035, 2381, 2619, 2711
BceAI	ACGGC	2 cut(s)	914, 2902
BciT130I	CCWGG	6 cut(s)	399, 794, 1247, 1798, 2192, 2583
BciVI	GTATCC	1 cut(s)	2079
BclI	TGATCA	3 cut(s)	30, 202, 1641
BcnI	CCSGG	2 cut(s)	2141, 2321
BcoDI	GTCTC	3 cut(s)	574, 1753, 2025
BfaI	CTAG	5 cut(s)	393, 572, 767, 1059, 1428
BfmI	CTRYAG	6 cut(s)	2080, 2589, 2616, 2638, 2849, 2895
BfrI	CTTAAG	1 cut(s)	2024
BfuI	GTATCC	1 cut(s)	2079
BglI	GCCNNNNNGGC	1 cut(s)	93
BglII	AGATCT	2 cut(s)	1945, 2845
BisI	GCNGC	9 cut(s)	224, 993, 2080, 2175, 2510, 2641, 2814, 2877, 2895
BlnI	CCTAGG	3 cut(s)	392, 571, 1427
BlsI	GCNGC	9 cut(s)	225, 994, 2081, 2176, 2511, 2642, 2815, 2878, 2896
BmcAI	AGTACT	1 cut(s)	304
Bme1390I	CCNGG	8 cut(s)	399, 794, 1247, 1798, 2141, 2192, 2321, 2583
Bme18I	GGWCC	2 cut(s)	1908, 2284
BmeT110I	CYCGRG	1 cut(s)	735
BmgBI	CACGTC	1 cut(s)	2131
BmgT120I	GGNCC	4 cut(s)	101, 799, 1908, 2284
BmiI	GGNNCC	7 cut(s)	75, 1909, 1910, 2286, 2466, 2513, 2841
BmrFI	CCNGG	8 cut(s)	399, 794, 1247, 1798, 2141, 2192, 2321, 2583
BmrI	ACTGGG	1 cut(s)	56
BmsI	GCATC	5 cut(s)	775, 1063, 1850, 2059, 2425
BmuI	ACTGGG	1 cut(s)	56
BplI	GAGNNNNNCTC	2 cut(s)	1693, 1725
BpmI	CTGGAG	3 cut(s)	300, 1436, 2928
Bpu10I	CCTNAGC	2 cut(s)	1803, 2880
Bpu14I	TTCGAA	1 cut(s)	1144
BpuEI	CTTGAG	2 cut(s)	1590, 2220
BpuMI	CCSGG	2 cut(s)	2141, 2321
Bsa29I	ATCGAT	1 cut(s)	684
BsaBI	GATNNNNATC	2 cut(s)	1511, 2631
BsaJI	CCNNGG	8 cut(s)	345, 392, 571, 792, 1332, 1427, 1559, 2190
BsaXI	ACNNNNNCTCC	8 cut(s)	136, 166, 175, 205, 908, 938, 2115, 2145
Bse118I	RCCGGY	2 cut(s)	770, 1662
Bse1I	ACTGG	7 cut(s)	62, 88, 549, 760, 2160, 2483, 2725
Bse3DI	GCAATG	1 cut(s)	1970
Bse8I	GATNNNNATC	2 cut(s)	1511, 2631
BseBI	CCWGG	6 cut(s)	399, 794, 1247, 1798, 2192, 2583
BseCI	ATCGAT	1 cut(s)	684
BseDI	CCNNGG	8 cut(s)	345, 392, 571, 792, 1332, 1427, 1559, 2190
BseGI	GGATG	6 cut(s)	1446, 1841, 2074, 2392, 2611, 2701
BseJI	GATNNNNATC	2 cut(s)	1511, 2631
BseMI	GCAATG	1 cut(s)	1970
BseMII	CTCAG	4 cut(s)	27, 149, 1794, 2380
BseNI	ACTGG	7 cut(s)	62, 88, 549, 760, 2160, 2483, 2725
BseRI	GAGGAG	1 cut(s)	297
BseSI	GKGCMC	3 cut(s)	2768, 2827, 2888
BseX3I	CGGCCG	1 cut(s)	2915
BseXI	GCAGC	7 cut(s)	979, 2066, 2161, 2652, 2800, 2863, 2881
BseYI	CCCAGC	3 cut(s)	413, 1032, 2219
BsgI	GTGCAG	2 cut(s)	226, 1715
Bsh1236I	CGCG	1 cut(s)	2770
Bsh1285I	CGRYCG	1 cut(s)	2918
BshNI	GGYRCC	2 cut(s)	73, 2511
BshVI	ATCGAT	1 cut(s)	684
BsiEI	CGRYCG	1 cut(s)	2918
BsiHKAI	GWGCWC	5 cut(s)	14, 834, 1878, 2236, 2768
BsiHKCI	CYCGRG	1 cut(s)	735
BsiSI	CCGG	7 cut(s)	771, 1663, 2141, 2201, 2320, 2792, 2914
BslFI	GGGAC	5 cut(s)	837, 1894, 2148, 2558, 2674
BsmAI	GTCTC	3 cut(s)	574, 1753, 2025
BsmFI	GGGAC	5 cut(s)	837, 1894, 2148, 2558, 2674
BsmI	GAATGC	1 cut(s)	1474
BsoBI	CYCGRG	1 cut(s)	735
Bsp119I	TTCGAA	1 cut(s)	1144
Bsp1286I	GDGCHC	7 cut(s)	14, 834, 1878, 2236, 2768, 2827, 2888
Bsp1407I	TGTACA	2 cut(s)	555, 2268
Bsp19I	CCATGG	2 cut(s)	345, 1332
BspACI	CCGC	6 cut(s)	223, 588, 1302, 2177, 2480, 2509
BspCNI	CTCAG	4 cut(s)	26, 148, 1795, 2379
BspDI	ATCGAT	1 cut(s)	684
BspFNI	CGCG	1 cut(s)	2770
BspHI	TCATGA	1 cut(s)	1573
BspLI	GGNNCC	7 cut(s)	75, 1909, 1910, 2286, 2466, 2513, 2841
BspMAI	CTGCAG	4 cut(s)	2084, 2642, 2853, 2899
BspPI	GGATC	5 cut(s)	858, 1440, 1514, 2311, 2331
BspT104I	TTCGAA	1 cut(s)	1144
BspT107I	GGYRCC	2 cut(s)	73, 2511
BspTI	CTTAAG	1 cut(s)	2024
BsrDI	GCAATG	1 cut(s)	1970
BsrFI	RCCGGY	2 cut(s)	770, 1662
BsrGI	TGTACA	2 cut(s)	555, 2268
BsrI	ACTGG	7 cut(s)	62, 88, 549, 760, 2160, 2483, 2725
BssAI	RCCGGY	2 cut(s)	770, 1662
BssECI	CCNNGG	8 cut(s)	345, 392, 571, 792, 1332, 1427, 1559, 2190
BssNAI	GTATAC	1 cut(s)	615
BssSI	CACGAG	1 cut(s)	1710
BssT1I	CCWWGG	6 cut(s)	345, 392, 571, 1332, 1427, 1559
Bst1107I	GTATAC	1 cut(s)	615
Bst2BI	CACGAG	1 cut(s)	1710
Bst2UI	CCWGG	6 cut(s)	399, 794, 1247, 1798, 2192, 2583
Bst4CI	ACNGT	9 cut(s)	247, 851, 1082, 1459, 1657, 2004, 2059, 2302, 2590
Bst6I	CTCTTC	2 cut(s)	1014, 2939
BstAFI	CTTAAG	1 cut(s)	2024
BstAPI	GCANNNNNTGC	1 cut(s)	2801
BstAUI	TGTACA	2 cut(s)	555, 2268
BstBI	TTCGAA	1 cut(s)	1144
BstC8I	GCNNGC	6 cut(s)	588, 1342, 1527, 2673, 2768, 2925
BstDEI	CTNAG	7 cut(s)	13, 135, 159, 1103, 1803, 2366, 2880
BstDSI	CCRYGG	2 cut(s)	345, 1332
BstENI	CCTNNNNNAGG	1 cut(s)	1608
BstF5I	GGATG	6 cut(s)	1446, 1841, 2074, 2392, 2611, 2701
BstFNI	CGCG	1 cut(s)	2770
BstHHI	GCGC	2 cut(s)	2772, 2806
BstMAI	GTCTC	3 cut(s)	574, 1753, 2025
BstMCI	CGRYCG	1 cut(s)	2918
BstMWI	GCNNNNNNNGC	8 cut(s)	93, 536, 992, 2183, 2801, 2810, 2822, 2848
BstNI	CCWGG	6 cut(s)	399, 794, 1247, 1798, 2192, 2583
BstNSI	RCATGY	4 cut(s)	748, 1419, 1832, 1985
BstSCI	CCNGG	8 cut(s)	397, 792, 1245, 1796, 2139, 2190, 2319, 2581
BstSFI	CTRYAG	6 cut(s)	2080, 2589, 2616, 2638, 2849, 2895
BstSLI	GKGCMC	3 cut(s)	2768, 2827, 2888
BstUI	CGCG	1 cut(s)	2770
BstV1I	GCAGC	7 cut(s)	979, 2066, 2161, 2652, 2800, 2863, 2881
BstX2I	RGATCY	4 cut(s)	863, 1945, 2323, 2845
BstXI	CCANNNNNNTGG	2 cut(s)	352, 1560
BstYI	RGATCY	4 cut(s)	863, 1945, 2323, 2845
BstZ17I	GTATAC	1 cut(s)	615
BstZI	CGGCCG	1 cut(s)	2915
Bsu15I	ATCGAT	1 cut(s)	684
BsuI	GTATCC	1 cut(s)	2079
BsuTUI	ATCGAT	1 cut(s)	684
BtgI	CCRYGG	2 cut(s)	345, 1332
BtrI	CACGTC	1 cut(s)	2131
BtsCI	GGATG	6 cut(s)	1446, 1841, 2074, 2392, 2611, 2701
BtsI	GCAGTG	2 cut(s)	238, 2560
BtsIMutI	CAGTG	9 cut(s)	238, 1522, 1622, 2055, 2104, 2311, 2490, 2560, 2586
Cac8I	GCNNGC	6 cut(s)	588, 1342, 1527, 2673, 2768, 2925
CaiI	CAGNNNCTG	3 cut(s)	1039, 1775, 2254
CciI	TCATGA	1 cut(s)	1573
CfoI	GCGC	2 cut(s)	2772, 2806
Cfr10I	RCCGGY	2 cut(s)	770, 1662
Cfr13I	GGNCC	4 cut(s)	101, 799, 1908, 2284
ClaI	ATCGAT	1 cut(s)	684
Csp6I	GTAC	7 cut(s)	303, 556, 847, 1052, 2269, 2501, 2786
CviQI	GTAC	7 cut(s)	303, 556, 847, 1052, 2269, 2501, 2786
DdeI	CTNAG	7 cut(s)	13, 135, 159, 1103, 1803, 2366, 2880
DraI	TTTAAA	1 cut(s)	2931
DraIII	CACNNNGTG	2 cut(s)	28, 1691
EaeI	YGGCCR	6 cut(s)	85, 224, 688, 2198, 2669, 2915
EagI	CGGCCG	1 cut(s)	2915
Eam1104I	CTCTTC	2 cut(s)	1014, 2939
EarI	CTCTTC	2 cut(s)	1014, 2939
EciI	GGCGGA	1 cut(s)	1317
Ecl136II	GAGCTC	3 cut(s)	12, 832, 1876
EclXI	CGGCCG	1 cut(s)	2915
Eco130I	CCWWGG	6 cut(s)	345, 392, 571, 1332, 1427, 1559
Eco147I	AGGCCT	5 cut(s)	397, 949, 1340, 1426, 1801
Eco24I	GRGCYC	3 cut(s)	14, 834, 1878
Eco32I	GATATC	1 cut(s)	1924
Eco47I	GGWCC	2 cut(s)	1908, 2284
Eco52I	CGGCCG	1 cut(s)	2915
Eco53kI	GAGCTC	3 cut(s)	12, 832, 1876
Eco57I	CTGAAG	2 cut(s)	972, 1515
Eco88I	CYCGRG	1 cut(s)	735
EcoICRI	GAGCTC	3 cut(s)	12, 832, 1876
EcoNI	CCTNNNNNAGG	1 cut(s)	1608
EcoO109I	RGGNCCY	3 cut(s)	799, 1908, 2284
EcoRI	GAATTC	1 cut(s)	1545
EcoRII	CCWGG	6 cut(s)	397, 792, 1245, 1796, 2190, 2581
EcoRV	GATATC	1 cut(s)	1924
EcoT14I	CCWWGG	6 cut(s)	345, 392, 571, 1332, 1427, 1559
EcoT22I	ATGCAT	1 cut(s)	1843
EcoT38I	GRGCYC	3 cut(s)	14, 834, 1878
ErhI	CCWWGG	6 cut(s)	345, 392, 571, 1332, 1427, 1559
FalI	AAGNNNNNCTT	2 cut(s)	1266, 1298
FaqI	GGGAC	5 cut(s)	837, 1894, 2148, 2558, 2674
FauI	CCCGC	1 cut(s)	595
FauNDI	CATATG	1 cut(s)	2755
FbaI	TGATCA	3 cut(s)	30, 202, 1641
FblI	GTMKAC	2 cut(s)	614, 2559
Fnu4HI	GCNGC	9 cut(s)	224, 993, 2080, 2175, 2510, 2641, 2814, 2877, 2895
FokI	GGATG	6 cut(s)	1433, 1828, 2081, 2399, 2598, 2708
FriOI	GRGCYC	3 cut(s)	14, 834, 1878
Fsp4HI	GCNGC	9 cut(s)	224, 993, 2080, 2175, 2510, 2641, 2814, 2877, 2895
FspAI	RTGCGCAY	1 cut(s)	2805
FspBI	CTAG	5 cut(s)	393, 572, 767, 1059, 1428
FspI	TGCGCA	1 cut(s)	2805
GlaI	GCGC	2 cut(s)	2771, 2805
GluI	GCNGC	9 cut(s)	224, 993, 2080, 2175, 2510, 2641, 2814, 2877, 2895
GsaI	CCCAGC	3 cut(s)	417, 1036, 2223
GsuI	CTGGAG	3 cut(s)	300, 1436, 2928
HapII	CCGG	7 cut(s)	771, 1663, 2141, 2201, 2320, 2792, 2914
HhaI	GCGC	2 cut(s)	2772, 2806
Hin6I	GCGC	2 cut(s)	2770, 2804
HinP1I	GCGC	2 cut(s)	2770, 2804
HindIII	AAGCTT	2 cut(s)	1523, 1818
HpaII	CCGG	7 cut(s)	771, 1663, 2141, 2201, 2320, 2792, 2914
Hpy166II	GTNNAC	5 cut(s)	523, 615, 1727, 2560, 2766
Hpy8I	GTNNAC	5 cut(s)	523, 615, 1727, 2560, 2766
HpyCH4III	ACNGT	9 cut(s)	247, 851, 1082, 1459, 1657, 2004, 2059, 2302, 2590
HpyCH4IV	ACGT	1 cut(s)	2130
HpyF10VI	GCNNNNNNNGC	8 cut(s)	93, 536, 992, 2183, 2801, 2810, 2822, 2848
HpyF3I	CTNAG	7 cut(s)	13, 135, 159, 1103, 1803, 2366, 2880
HpySE526I	ACGT	1 cut(s)	2130
HspAI	GCGC	2 cut(s)	2770, 2804
KflI	GGGWCCC	1 cut(s)	1908
Ksp22I	TGATCA	3 cut(s)	30, 202, 1641
LmnI	GCTCC	3 cut(s)	793, 2123, 2839
Lsp1109I	GCAGC	7 cut(s)	979, 2066, 2161, 2652, 2800, 2863, 2881
LweI	GCATC	5 cut(s)	775, 1063, 1850, 2059, 2425
MaeI	CTAG	5 cut(s)	393, 572, 767, 1059, 1428
MaeII	ACGT	1 cut(s)	2130
MaeIII	GTNAC	7 cut(s)	456, 505, 1123, 1411, 2131, 2206, 2485
MflI	RGATCY	4 cut(s)	863, 1945, 2323, 2845
MhlI	GDGCHC	7 cut(s)	14, 834, 1878, 2236, 2768, 2827, 2888
MlsI	TGGCCA	1 cut(s)	87
MluNI	TGGCCA	1 cut(s)	87
MlyI	GAGTC	6 cut(s)	323, 616, 926, 2128, 2912, 2919
MmeI	TCCRAC	3 cut(s)	173, 179, 894
Mox20I	TGGCCA	1 cut(s)	87
Mph1103I	ATGCAT	1 cut(s)	1843
MscI	TGGCCA	1 cut(s)	87
MslI	CAYNNNNRTG	3 cut(s)	350, 483, 2682
Msp20I	TGGCCA	1 cut(s)	87
MspA1I	CMGCKG	1 cut(s)	992
MspCI	CTTAAG	1 cut(s)	2024
MspI	CCGG	7 cut(s)	771, 1663, 2141, 2201, 2320, 2792, 2914
MspR9I	CCNGG	8 cut(s)	399, 794, 1247, 1798, 2141, 2192, 2321, 2583
Mva1269I	GAATGC	1 cut(s)	1474
MvaI	CCWGG	6 cut(s)	399, 794, 1247, 1798, 2192, 2583
MvnI	CGCG	1 cut(s)	2770
MwoI	GCNNNNNNNGC	8 cut(s)	93, 536, 992, 2183, 2801, 2810, 2822, 2848
NciI	CCSGG	2 cut(s)	2141, 2321
NcoI	CCATGG	2 cut(s)	345, 1332
NdeI	CATATG	1 cut(s)	2755
NlaIV	GGNNCC	7 cut(s)	75, 1909, 1910, 2286, 2466, 2513, 2841
NmuCI	GTSAC	3 cut(s)	2131, 2206, 2485
NsbI	TGCGCA	1 cut(s)	2805
NsiI	ATGCAT	1 cut(s)	1843
NspI	RCATGY	4 cut(s)	748, 1419, 1832, 1985
NspV	TTCGAA	1 cut(s)	1144
PaeR7I	CTCGAG	1 cut(s)	735
PagI	TCATGA	1 cut(s)	1573
PceI	AGGCCT	5 cut(s)	397, 949, 1340, 1426, 1801
PciI	ACATGT	3 cut(s)	744, 1415, 1828
PcsI	WCGNNNNNNNCGW	1 cut(s)	2127
PctI	GAATGC	1 cut(s)	1474
PfeI	GAWTC	5 cut(s)	563, 681, 1260, 2721, 2829
PflFI	GACNNNGTC	1 cut(s)	1066
PflMI	CCANNNNNTGG	1 cut(s)	1039
PfoI	TCCNGGA	2 cut(s)	1245, 2319
PkrI	GCNGC	9 cut(s)	225, 994, 2081, 2176, 2511, 2642, 2815, 2878, 2896
PleI	GAGTC	6 cut(s)	322, 615, 925, 2128, 2911, 2918
PpsI	GAGTC	6 cut(s)	322, 615, 925, 2128, 2911, 2918
PpuMI	RGGWCCY	2 cut(s)	1908, 2284
PscI	ACATGT	3 cut(s)	744, 1415, 1828
PshBI	ATTAAT	1 cut(s)	1955
Psp124BI	GAGCTC	3 cut(s)	14, 834, 1878
Psp5II	RGGWCCY	2 cut(s)	1908, 2284
Psp6I	CCWGG	6 cut(s)	397, 792, 1245, 1796, 2190, 2581
PspFI	CCCAGC	3 cut(s)	413, 1032, 2219
PspGI	CCWGG	6 cut(s)	397, 792, 1245, 1796, 2190, 2581
PspN4I	GGNNCC	7 cut(s)	75, 1909, 1910, 2286, 2466, 2513, 2841
PspPI	GGNCC	4 cut(s)	101, 799, 1908, 2284
PspPPI	RGGWCCY	2 cut(s)	1908, 2284
PspXI	VCTCGAGB	1 cut(s)	735
PstI	CTGCAG	4 cut(s)	2084, 2642, 2853, 2899
PstNI	CAGNNNCTG	3 cut(s)	1039, 1775, 2254
PsuI	RGATCY	4 cut(s)	863, 1945, 2323, 2845
PsyI	GACNNNGTC	1 cut(s)	1066
PvuII	CAGCTG	1 cut(s)	992
RsaI	GTAC	7 cut(s)	304, 557, 848, 1053, 2270, 2502, 2787
RsaNI	GTAC	7 cut(s)	303, 556, 847, 1052, 2269, 2501, 2786
RseI	CAYNNNNRTG	3 cut(s)	350, 483, 2682
SacI	GAGCTC	3 cut(s)	14, 834, 1878
SatI	GCNGC	9 cut(s)	224, 993, 2080, 2175, 2510, 2641, 2814, 2877, 2895
Sau96I	GGNCC	4 cut(s)	101, 799, 1908, 2284
ScaI	AGTACT	1 cut(s)	304
SchI	GAGTC	6 cut(s)	323, 616, 926, 2128, 2912, 2919
ScrFI	CCNGG	8 cut(s)	399, 794, 1247, 1798, 2141, 2192, 2321, 2583
SduI	GDGCHC	7 cut(s)	14, 834, 1878, 2236, 2768, 2827, 2888
SfaNI	GCATC	5 cut(s)	775, 1063, 1850, 2059, 2425
SfcI	CTRYAG	6 cut(s)	2080, 2589, 2616, 2638, 2849, 2895
Sfr274I	CTCGAG	1 cut(s)	735
SfuI	TTCGAA	1 cut(s)	1144
SinI	GGWCC	2 cut(s)	1908, 2284
SlaI	CTCGAG	1 cut(s)	735
SmiMI	CAYNNNNRTG	3 cut(s)	350, 483, 2682
SmlI	CTYRAG	4 cut(s)	735, 1605, 2024, 2235
SmoI	CTYRAG	4 cut(s)	735, 1605, 2024, 2235
SseBI	AGGCCT	5 cut(s)	397, 949, 1340, 1426, 1801
SsiI	CCGC	6 cut(s)	223, 588, 1302, 2177, 2480, 2509
SspI	AATATT	1 cut(s)	976
SspMI	CTAG	5 cut(s)	393, 572, 767, 1059, 1428
SstI	GAGCTC	3 cut(s)	14, 834, 1878
StuI	AGGCCT	5 cut(s)	397, 949, 1340, 1426, 1801
StyD4I	CCNGG	8 cut(s)	397, 792, 1245, 1796, 2139, 2190, 2319, 2581
StyI	CCWWGG	6 cut(s)	345, 392, 571, 1332, 1427, 1559
TaaI	ACNGT	9 cut(s)	247, 851, 1082, 1459, 1657, 2004, 2059, 2302, 2590
TaiI	ACGT	1 cut(s)	2133
TaqI	TCGA	7 cut(s)	512, 684, 736, 1144, 1156, 1543, 2168
TatI	WGTACW	5 cut(s)	302, 555, 1051, 2268, 2500
TauI	GCSGC	2 cut(s)	226, 2512
TfiI	GAWTC	5 cut(s)	563, 681, 1260, 2721, 2829
TscAI	CASTG	9 cut(s)	245, 1522, 1622, 2062, 2104, 2311, 2490, 2560, 2593
TseFI	GTSAC	3 cut(s)	2131, 2206, 2485
TseI	GCWGC	7 cut(s)	992, 2079, 2174, 2640, 2813, 2876, 2894
Tsp45I	GTSAC	3 cut(s)	2131, 2206, 2485
TspDTI	ATGAA	8 cut(s)	17, 684, 1238, 1398, 1590, 1643, 1862, 2482
TspGWI	ACGGA	1 cut(s)	1702
TspRI	CASTG	9 cut(s)	245, 1522, 1622, 2062, 2104, 2311, 2490, 2560, 2593
Tth111I	GACNNNGTC	1 cut(s)	1066
Van91I	CCANNNNNTGG	1 cut(s)	1039
Vha464I	CTTAAG	1 cut(s)	2024
VneI	GTGCAC	1 cut(s)	2764
VpaK11BI	GGWCC	2 cut(s)	1908, 2284
VspI	ATTAAT	1 cut(s)	1955
XagI	CCTNNNNNAGG	1 cut(s)	1608
XapI	RAATTY	6 cut(s)	334, 725, 807, 1545, 1760, 2621
XceI	RCATGY	4 cut(s)	748, 1419, 1832, 1985
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1253
XhoI	CTCGAG	1 cut(s)	735
XmaJI	CCTAGG	3 cut(s)	392, 571, 1427
XmiI	GTMKAC	2 cut(s)	614, 2559
XspI	CTAG	5 cut(s)	393, 572, 767, 1059, 1428
ZrmI	AGTACT	1 cut(s)	304
Zsp2I	ATGCAT	1 cut(s)	1843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.