Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003981

Physical Location & Seq

Genomic Coordinates Reverse (-)

18193037 .. 18201455

Gene Structure 8419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1201200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGCCTATAAGAAAGAAAGAGAGCTCGGAGCAACACCTTGTGATCAAACCCCACTTGCAGAACCCAGTGAACCCAGTTCAGAAGGCACCGAAAACTGCCCAAAATGGCAAAGGTCCACCACCCCAGGAATCCCAAAAAGCCAAACCCCATAACCAAACTTCACCCCCAACAAAAAACAGAGGAAGAAGAAGGGGCAGAGGTGGTCGGAAGTCTGATCAAGGCGATGTTTGTATGAGGCCTAGTTCGCGGCACTGCACGGTGGCTCATCTGCCTGCCTCACCAAGCTTGGGTGGTGCTGCTTCTGTAGCGAATGCTCCAAATGGGTATGGCGGCAACTTAGTTTCCATGGATATGGGGTTTCCGACTTCAAGCAAGTCATTGAGCTTTGCTCCTCGGCCTGGTTATGGACAAGTTGGGATCAAGTGTGTTGTGAAGGCCAACCATTTCTTTGCAGAGTTACCAGAGAAGGACTTGAACCATTATGATGTTAGAATAACTCCCGAAGTTGCATCGACAGCTGTGAACAGAGCTATCATGGCAGAACTGGTGAGGTTGTACAAGGAATCTGACTTAGGAATGAGACTGCCTGCTTATGATGGTAGGAAGAGTCTGTACACTGCTGGTGAGCTGCCCTTTGCTTGGAAGGAGTTCAATATTAAGCTTGTTGAGGAACAGGATGGAATCGTTGGCCGAACAAGAGAAAGAGTATATAATGTGGTGATTAAATTTGTTGCACGAGCAAACATGCATCATCTGGGTCAGTTTCTGGCCGGTAAGTGTGCTGATGCTCCACAAGAAGCCCTCCAAATTCTTGACATTGTATTACGAGAGCTCTCGAACAAGAGGTACTGCCCCATCGGGAGATCATTCTTTTCACCTGATATCAGAACACCACAACGGCTTGGTGAAGGGTTGGAATCATGGTGCGGCTTTTATCAAAGTATAAGACCTACCCAAATGGGTCTTTCCCTCAATATTGATATGGCTTCAGCTGCATTTATTGAGCCTCTCCCTGTAATAGAGTTTGTAGCGCAGCTTCTAGGCAAAGATGTATTGTCTAGGACATTGTCTGATGCTGACCGTGTAAAGATTAAAAAGGCTCTTAGAGGTGTGAAAGTTGAAGTTACACACAGAGGGAGTGTTCGTAGAAAGTATCGAGTTTCAGGATTGACATCTCAACCTACAAGAGAACTAGTGTTTCCTGTTGATGAAAACTTGACCATGAAGTCCGTAATTGAATACTTCCAGGAGATGTATGGGTTCACCATTCAACACGGGCATCTTCCTTGCCTTCAAGTTGGTAACCAGAAGAAGGCAAACTATTTACCCATGGAGGCCTGCAAAATTGTTGAGGGGCAACGGTATACAAAAAGGTTGAATGAGAAGCAAATTACTGCTCTCTTAAAGGTTACATGTCAAAGACCTAGGGATCGTGAAAATGACATCCTACAGACTGTTCAACACAATGCTTATGACCAAGATCCTTACGCGAAGGAGTTTGGAATCAAAATCAGTGAAAAACTAGCTTCGGTCGAAGCTCGAATTCTTCCTGCGCCTTGGCTGAAGTATCATGAAACTGGAAAAGAAAAGAATTGTTTGCCTCAAGTTGGTCAGTGGAATATGATGAACAAGAAGATGATTAATGGAATGACCGTCAGCCGGTGGGCTTGTATTAATTTTTCACGGGGTGTGCAAGAGAGTGTTGCACGTGGGTTTTGCAGCGAACTTGCTCAAATGTGTCAAGTATCTGGCATGGAATTTAATCCAGAACCTGTAATTCCAATCCACAATGCTAGGCCTGAGCAAGTAGAGAAAGCTTTGAAGCATGTTTATCATGCATCCATGAACAAGACCAAAGGAAAAGATTTAGAGCTCTTGTTAGCCATTTTGCCTGACAACAACGGGTCCCTGTATGGTGATATCAAGCGAATATGTGAAACAGATCTCGGTTTAATATCACAATGCTGTCTCACAAAGTATGTTTTCAAGATCAGCAAACAGTACTTGGCTAATGTCTCCCTAAAGATAAATGTTAAGATGGGTGGCAGAAACACTGTTCTTTTGGATGCTATCAGCTGCAGGATACCTTTGGTCAGTGATATTCCAACCATAATCTTCGGAGCTGATGTGACACACCCTGAAAATGGAGAAGATTCCAGTCCCTCAATTGCTGCTGTCGTAGCTTCCCAGGACTGGCCCGAAGTGACCAAGTATGCTGGATTAGTTTGTGCTCAAGCTCACAGACAAGAACTCATACAAGATTTGTATAAAACATGGCAAGACCCTGTTCGTGGCACGGTCAGTGGCGGCATGATCCGGGATCTTCTGGTTTCCTTTAGGAAAGCAACAGGACAGAAGCCACTAAGGATTATATTTTACAGGGATGGCGTAAGTGAAGGTCAATTTTATCAAGTCTTACTATACGAGCTCGATGCAATCCGGAAGGCATGCGCTTCTCTGGAACCAAACTATCAACCACCAGTGACTTTCATTGTAGTTCAAAAACGGCATCACACACGATTATTTGCTAATAACCATAGGGACAGGAGCAGCGTAGACAAGAGTGGGAACATATTACCTGGCACCGTGGTTGATACTAAGATTTGTCATCCAACCGAACATGATTTTTATCTCTGCAGTCATGCTGGTATTCAGGGGACAAGTCGCCCAGCTCACTACCATGTTCTCTGGGATGAGAATAACTTTACTGCTGATGGAATCCAGTCATTGACAAACAACCTTTGTTACACATATGCAAGGTGCACGCGCTCTGTCTCTGTTGTACCTCCGGCATATTATGCACATTTGGCTGCTTTCCGTGCTCGATTCTATCTGGAGCCAGATATGCAAGAGAATGGTTCTACTGGTCACACTGCTAAGGGTACACGCGCTACTGGAGAGTCTGGAGTCCGACCATTACCAGCCTTGAAGGAGAATGTAAAGAGAGTAATGTTTTATTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0010586 GO:0016070 GO:0019827 GO:0032501 GO:0032502 GO:0034641 GO:0034660 GO:0035019 GO:0035198 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0046483 GO:0048509 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051704 GO:0051707 GO:0061980 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0098542 GO:0098727 GO:1901360 GO:1901363 GO:1902183

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

988

Amino Acids

110.4

Weight (kDa)

9.31

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	141 - 275	1.2e-33	N-terminal domain of argonaute
ArgoL1	PF08699	285 - 335	5.3e-19	Argonaute linker 1 domain
PAZ	PF02170	359 - 467	2.4e-28	PAZ domain
ArgoL2	PF16488	477 - 523	1e-16	Argonaute linker 2 domain
ArgoMid	PF16487	534 - 611	4.2e-13	Mid domain of argonaute
Piwi	PF02171	626 - 944	1.6e-115	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43810 AT5G43810 AT5G43810 AT5G43810
fragaria_vesca	FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630 FvH4_4g01630
malus_domestica	MD13G1201400.v1.1 MD16G1201200.v1.1
prunus_persica	Prupe.1G022900_v2.0.a1 Prupe.1G022900_v2.0.a1
pyrus_communis	pycom13g17460 pycom16g16970
rosa_chinensis	RchiOBHm_Chr4g0388221 RchiOBHm_Chr4g0388231
rosa_laevigata	RLG00000010073
rosa_roxburghii	Rroxscaffold_5G00334710
rosa_rugosa	Rorug03G0316100 Rorug03G0316200 Rorug03G0316300 Rorug03G0316300 Rorug03G0316300 Rorug03G0316300 Rorug03G0316400
rosa_samantha	Rh4AG021700 Rh4BG015800 Rh4CG021900 Rh4DG016600
rosa_wichuraiana	Rw4G001420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1225
AccB1I	GGYRCC	2 cut(s)	85, 2582
AccI	GTMKAC	2 cut(s)	1364, 2556
AccII	CGCG	4 cut(s)	247, 1490, 2767, 2889
AccIII	TCCGGA	1 cut(s)	2439
AciI	CCGC	4 cut(s)	247, 330, 927, 2307
AclWI	GGATC	5 cut(s)	425, 1437, 1475, 2308, 2328
AcoI	YGGCCR	2 cut(s)	688, 768
AcsI	RAATTY	4 cut(s)	725, 807, 1542, 1757
AcuI	CTGAAG	2 cut(s)	972, 1583
AcvI	CACGTG	1 cut(s)	1709
AdeI	CACNNNGTG	2 cut(s)	40, 1688
AfaI	GTAC	6 cut(s)	557, 614, 848, 2003, 2784, 2884
AflIII	ACRYGT	1 cut(s)	1412
AhlI	ACTAGT	1 cut(s)	1192
AjnI	CCWGG	5 cut(s)	123, 397, 1245, 2187, 2578
Alw21I	GWGCWC	7 cut(s)	26, 834, 1875, 2233, 2430, 2765, 2824
Alw26I	GTCTC	4 cut(s)	574, 1973, 2020, 2779
Alw44I	GTGCAC	1 cut(s)	2761
AlwI	GGATC	5 cut(s)	425, 1437, 1475, 2308, 2328
AlwNI	CAGNNNCTG	2 cut(s)	766, 1772
Aor13HI	TCCGGA	1 cut(s)	2439
AoxI	GGCC	8 cut(s)	236, 395, 435, 688, 768, 1335, 1796, 2195
ApaLI	GTGCAC	1 cut(s)	2761
ApeKI	GCWGC	9 cut(s)	296, 628, 992, 1033, 1719, 2076, 2171, 2550, 2810
ApoI	RAATTY	4 cut(s)	725, 807, 1542, 1757
AseI	ATTAAT	2 cut(s)	1641, 1674
Asp700I	GAANNNNTTC	1 cut(s)	647
AspA2I	CCTAGG	1 cut(s)	1424
AspLEI	GCGC	5 cut(s)	1033, 1555, 2453, 2769, 2891
AspS9I	GGNCC	3 cut(s)	113, 1905, 2196
AsuC2I	CCSGG	1 cut(s)	2318
AsuHPI	GGTGA	9 cut(s)	153, 270, 559, 635, 730, 867, 917, 1255, 1928
AvaII	GGWCC	2 cut(s)	113, 1905
AvrII	CCTAGG	1 cut(s)	1424
BaeGI	GKGCMC	1 cut(s)	2765
BanI	GGYRCC	2 cut(s)	85, 2582
BanII	GRGCYC	4 cut(s)	26, 834, 1875, 2430
BarI	GAAGNNNNNNTAC	4 cut(s)	596, 628, 1020, 1052
BauI	CACGAG	1 cut(s)	735
BbrPI	CACGTG	1 cut(s)	1709
Bbv12I	GWGCWC	7 cut(s)	26, 834, 1875, 2233, 2430, 2765, 2824
BbvI	GCAGC	9 cut(s)	283, 615, 979, 1045, 1731, 2063, 2158, 2562, 2797
BccI	CCATC	6 cut(s)	590, 671, 863, 2032, 2378, 2708
BceAI	ACGGC	2 cut(s)	914, 2522
BcgI	CGANNNNNNTGC	2 cut(s)	2414, 2448
BciT130I	CCWGG	5 cut(s)	125, 399, 1247, 2189, 2580
BciVI	GTATCC	1 cut(s)	2076
BclI	TGATCA	2 cut(s)	42, 214
BcnI	CCSGG	1 cut(s)	2318
BcoDI	GTCTC	4 cut(s)	574, 1973, 2020, 2779
BcuI	ACTAGT	1 cut(s)	1192
BfaI	CTAG	7 cut(s)	240, 1040, 1059, 1193, 1425, 1523, 1794
BfmI	CTRYAG	4 cut(s)	303, 1448, 2077, 2635
BfuI	GTATCC	1 cut(s)	2076
BglII	AGATCT	1 cut(s)	1942
BlnI	CCTAGG	1 cut(s)	1424
BmcAI	AGTACT	1 cut(s)	2003
Bme1390I	CCNGG	6 cut(s)	125, 399, 1247, 2189, 2318, 2580
Bme18I	GGWCC	2 cut(s)	113, 1905
BmgT120I	GGNCC	3 cut(s)	113, 1905, 2196
BmiI	GGNNCC	6 cut(s)	87, 1906, 1907, 2463, 2584, 2838
BmrFI	CCNGG	6 cut(s)	125, 399, 1247, 2189, 2318, 2580
BmrI	ACTGGG	2 cut(s)	59, 68
BmsI	GCATC	9 cut(s)	518, 757, 775, 1063, 1288, 1847, 2056, 2422, 2518
BmuI	ACTGGG	2 cut(s)	59, 68
BplI	GAGNNNNNCTC	2 cut(s)	373, 405
BpmI	CTGGAG	3 cut(s)	2855, 2916, 2925
Bpu10I	CCTNAGC	2 cut(s)	1800, 2877
BpuEI	CTTGAG	2 cut(s)	1587, 2217
BpuMI	CCSGG	1 cut(s)	2318
BsaAI	YACGTR	1 cut(s)	1709
BsaBI	GATNNNNATC	2 cut(s)	1171, 2628
BsaJI	CCNNGG	8 cut(s)	123, 345, 392, 1329, 1424, 1556, 2187, 2586
BsaWI	WCCGGW	1 cut(s)	2439
BsaXI	ACNNNNNCTCC	4 cut(s)	1129, 1159, 2112, 2142
Bse118I	RCCGGY	2 cut(s)	770, 1659
Bse8I	GATNNNNATC	2 cut(s)	1171, 2628
BseAI	TCCGGA	1 cut(s)	2439
BseBI	CCWGG	5 cut(s)	125, 399, 1247, 2189, 2580
BseDI	CCNNGG	8 cut(s)	123, 345, 392, 1329, 1424, 1556, 2187, 2586
BseGI	GGATG	7 cut(s)	682, 1443, 1838, 2071, 2389, 2608, 2698
BseJI	GATNNNNATC	2 cut(s)	1171, 2628
BseMII	CTCAG	1 cut(s)	1791
BseRI	GAGGAG	1 cut(s)	381
BseSI	GKGCMC	1 cut(s)	2765
BseXI	GCAGC	9 cut(s)	283, 615, 979, 1045, 1731, 2063, 2158, 2562, 2797
BseYI	CCCAGC	1 cut(s)	2668
BsgI	GTGCAG	1 cut(s)	238
Bsh1236I	CGCG	4 cut(s)	247, 1490, 2767, 2889
Bsh1285I	CGRYCG	1 cut(s)	1533
BshFI	GGCC	8 cut(s)	238, 397, 437, 690, 770, 1337, 1798, 2197
BshNI	GGYRCC	2 cut(s)	85, 2582
BsiEI	CGRYCG	1 cut(s)	1533
BsiHKAI	GWGCWC	7 cut(s)	26, 834, 1875, 2233, 2430, 2765, 2824
BsiSI	CCGG	5 cut(s)	771, 1660, 2317, 2440, 2789
BslFI	GGGAC	4 cut(s)	1891, 2145, 2555, 2671
BsmAI	GTCTC	4 cut(s)	574, 1973, 2020, 2779
BsmFI	GGGAC	4 cut(s)	1891, 2145, 2555, 2671
BsmI	GAATGC	1 cut(s)	316
BsnI	GGCC	8 cut(s)	238, 397, 437, 690, 770, 1337, 1798, 2197
Bsp1286I	GDGCHC	7 cut(s)	26, 834, 1875, 2233, 2430, 2765, 2824
Bsp13I	TCCGGA	1 cut(s)	2439
Bsp1407I	TGTACA	2 cut(s)	555, 612
Bsp19I	CCATGG	2 cut(s)	345, 1329
BspACI	CCGC	4 cut(s)	247, 330, 927, 2307
BspANI	GGCC	8 cut(s)	238, 397, 437, 690, 770, 1337, 1798, 2197
BspCNI	CTCAG	1 cut(s)	1792
BspEI	TCCGGA	1 cut(s)	2439
BspFNI	CGCG	4 cut(s)	247, 1490, 2767, 2889
BspHI	TCATGA	1 cut(s)	1570
BspLI	GGNNCC	6 cut(s)	87, 1906, 1907, 2463, 2584, 2838
BspMAI	CTGCAG	2 cut(s)	2081, 2639
BspPI	GGATC	5 cut(s)	425, 1437, 1475, 2308, 2328
BspT107I	GGYRCC	2 cut(s)	85, 2582
BsrFI	RCCGGY	2 cut(s)	770, 1659
BsrGI	TGTACA	2 cut(s)	555, 612
BssAI	RCCGGY	2 cut(s)	770, 1659
BssECI	CCNNGG	8 cut(s)	123, 345, 392, 1329, 1424, 1556, 2187, 2586
BssNAI	GTATAC	1 cut(s)	1365
BssSI	CACGAG	1 cut(s)	735
BssT1I	CCWWGG	4 cut(s)	345, 1329, 1424, 1556
Bst1107I	GTATAC	1 cut(s)	1365
Bst2BI	CACGAG	1 cut(s)	735
Bst2UI	CCWGG	5 cut(s)	125, 399, 1247, 2189, 2580
Bst4CI	ACNGT	9 cut(s)	259, 1082, 1362, 1456, 1654, 2001, 2056, 2299, 2587
Bst6I	CTCTTC	1 cut(s)	599
BstAPI	GCANNNNNTGC	1 cut(s)	2798
BstAUI	TGTACA	2 cut(s)	555, 612
BstBAI	YACGTR	1 cut(s)	1709
BstC8I	GCNNGC	5 cut(s)	273, 588, 1339, 2449, 2765
BstDEI	CTNAG	7 cut(s)	337, 571, 1103, 1800, 2363, 2598, 2877
BstDSI	CCRYGG	3 cut(s)	345, 1329, 2586
BstEII	GGTNACC	1 cut(s)	1301
BstF5I	GGATG	7 cut(s)	682, 1443, 1838, 2071, 2389, 2608, 2698
BstFNI	CGCG	4 cut(s)	247, 1490, 2767, 2889
BstHHI	GCGC	5 cut(s)	1033, 1555, 2453, 2769, 2891
BstMAI	GTCTC	4 cut(s)	574, 1973, 2020, 2779
BstMCI	CGRYCG	1 cut(s)	1533
BstNI	CCWGG	5 cut(s)	125, 399, 1247, 2189, 2580
BstNSI	RCATGY	4 cut(s)	748, 1416, 1829, 2451
BstPI	GGTNACC	1 cut(s)	1301
BstSCI	CCNGG	6 cut(s)	123, 397, 1245, 2187, 2316, 2578
BstSFI	CTRYAG	4 cut(s)	303, 1448, 2077, 2635
BstSLI	GKGCMC	1 cut(s)	2765
BstUI	CGCG	4 cut(s)	247, 1490, 2767, 2889
BstV1I	GCAGC	9 cut(s)	283, 615, 979, 1045, 1731, 2063, 2158, 2562, 2797
BstX2I	RGATCY	3 cut(s)	1480, 1942, 2320
BstXI	CCANNNNNNTGG	1 cut(s)	352
BstYI	RGATCY	3 cut(s)	1480, 1942, 2320
BstZ17I	GTATAC	1 cut(s)	1365
BsuI	GTATCC	1 cut(s)	2076
BsuRI	GGCC	8 cut(s)	238, 397, 437, 690, 770, 1337, 1798, 2197
BtgI	CCRYGG	3 cut(s)	345, 1329, 2586
BtgZI	GCGATG	1 cut(s)	237
BtsCI	GGATG	7 cut(s)	682, 1443, 1838, 2071, 2389, 2608, 2698
BtsI	GCAGTG	3 cut(s)	250, 615, 2871
Cac8I	GCNNGC	5 cut(s)	273, 588, 1339, 2449, 2765
CaiI	CAGNNNCTG	2 cut(s)	766, 1772
CciI	TCATGA	1 cut(s)	1570
CfoI	GCGC	5 cut(s)	1033, 1555, 2453, 2769, 2891
Cfr10I	RCCGGY	2 cut(s)	770, 1659
Cfr13I	GGNCC	3 cut(s)	113, 1905, 2196
Csp6I	GTAC	6 cut(s)	556, 613, 847, 2002, 2783, 2883
CviQI	GTAC	6 cut(s)	556, 613, 847, 2002, 2783, 2883
DdeI	CTNAG	7 cut(s)	337, 571, 1103, 1800, 2363, 2598, 2877
DraIII	CACNNNGTG	2 cut(s)	40, 1688
DrdI	GACNNNNNNGTC	1 cut(s)	1225
DseDI	GACNNNNNNGTC	1 cut(s)	1225
EaeI	YGGCCR	2 cut(s)	688, 768
Eam1104I	CTCTTC	1 cut(s)	599
EarI	CTCTTC	1 cut(s)	599
Ecl136II	GAGCTC	4 cut(s)	24, 832, 1873, 2428
Eco130I	CCWWGG	4 cut(s)	345, 1329, 1424, 1556
Eco147I	AGGCCT	3 cut(s)	238, 1337, 1798
Eco24I	GRGCYC	4 cut(s)	26, 834, 1875, 2430
Eco32I	GATATC	2 cut(s)	883, 1921
Eco47I	GGWCC	2 cut(s)	113, 1905
Eco53kI	GAGCTC	4 cut(s)	24, 832, 1873, 2428
Eco57I	CTGAAG	2 cut(s)	972, 1583
Eco72I	CACGTG	1 cut(s)	1709
Eco91I	GGTNACC	1 cut(s)	1301
EcoICRI	GAGCTC	4 cut(s)	24, 832, 1873, 2428
EcoO109I	RGGNCCY	1 cut(s)	1905
EcoO65I	GGTNACC	1 cut(s)	1301
EcoRI	GAATTC	1 cut(s)	1542
EcoRII	CCWGG	5 cut(s)	123, 397, 1245, 2187, 2578
EcoRV	GATATC	2 cut(s)	883, 1921
EcoT14I	CCWWGG	4 cut(s)	345, 1329, 1424, 1556
EcoT22I	ATGCAT	2 cut(s)	750, 1840
EcoT38I	GRGCYC	4 cut(s)	26, 834, 1875, 2430
ErhI	CCWWGG	4 cut(s)	345, 1329, 1424, 1556
FaqI	GGGAC	4 cut(s)	1891, 2145, 2555, 2671
FauNDI	CATATG	1 cut(s)	2752
FbaI	TGATCA	2 cut(s)	42, 214
FblI	GTMKAC	2 cut(s)	1364, 2556
FokI	GGATG	7 cut(s)	689, 1430, 1825, 2078, 2396, 2595, 2705
FriOI	GRGCYC	4 cut(s)	26, 834, 1875, 2430
FspBI	CTAG	7 cut(s)	240, 1040, 1059, 1193, 1425, 1523, 1794
GlaI	GCGC	5 cut(s)	1032, 1554, 2452, 2768, 2890
GsaI	CCCAGC	1 cut(s)	2672
GsuI	CTGGAG	3 cut(s)	2855, 2916, 2925
HaeIII	GGCC	8 cut(s)	238, 397, 437, 690, 770, 1337, 1798, 2197
HapII	CCGG	5 cut(s)	771, 1660, 2317, 2440, 2789
HhaI	GCGC	5 cut(s)	1033, 1555, 2453, 2769, 2891
Hin6I	GCGC	5 cut(s)	1031, 1553, 2451, 2767, 2889
HinP1I	GCGC	5 cut(s)	1031, 1553, 2451, 2767, 2889
HindIII	AAGCTT	3 cut(s)	283, 659, 1815
HpaII	CCGG	5 cut(s)	771, 1660, 2317, 2440, 2789
HphI	GGTGA	9 cut(s)	153, 270, 559, 635, 730, 867, 917, 1255, 1928
Hpy166II	GTNNAC	9 cut(s)	70, 116, 523, 615, 1263, 1365, 2557, 2763, 2885
Hpy8I	GTNNAC	9 cut(s)	70, 116, 523, 615, 1263, 1365, 2557, 2763, 2885
HpyCH4III	ACNGT	9 cut(s)	259, 1082, 1362, 1456, 1654, 2001, 2056, 2299, 2587
HpyCH4IV	ACGT	1 cut(s)	1708
HpyF3I	CTNAG	7 cut(s)	337, 571, 1103, 1800, 2363, 2598, 2877
HpySE526I	ACGT	1 cut(s)	1708
HspAI	GCGC	5 cut(s)	1031, 1553, 2451, 2767, 2889
KflI	GGGWCCC	1 cut(s)	1905
Kpn2I	TCCGGA	1 cut(s)	2439
Ksp22I	TGATCA	2 cut(s)	42, 214
LmnI	GCTCC	7 cut(s)	28, 319, 394, 793, 2120, 2547, 2836
Lsp1109I	GCAGC	9 cut(s)	283, 615, 979, 1045, 1731, 2063, 2158, 2562, 2797
LweI	GCATC	9 cut(s)	518, 757, 775, 1063, 1288, 1847, 2056, 2422, 2518
MaeI	CTAG	7 cut(s)	240, 1040, 1059, 1193, 1425, 1523, 1794
MaeII	ACGT	1 cut(s)	1708
MaeIII	GTNAC	9 cut(s)	456, 1123, 1301, 1408, 2128, 2203, 2482, 2744, 2867
MfeI	CAATTG	1 cut(s)	2166
MflI	RGATCY	3 cut(s)	1480, 1942, 2320
MhlI	GDGCHC	7 cut(s)	26, 834, 1875, 2233, 2430, 2765, 2824
MlyI	GAGTC	3 cut(s)	616, 2909, 2916
MmeI	TCCRAC	6 cut(s)	185, 386, 894, 2129, 2636, 2935
Mph1103I	ATGCAT	2 cut(s)	750, 1840
MroI	TCCGGA	1 cut(s)	2439
MroXI	GAANNNNTTC	1 cut(s)	647
MseI	TTAA	9 cut(s)	657, 723, 1092, 1403, 1641, 1674, 1761, 1952, 2034
MslI	CAYNNNNRTG	3 cut(s)	350, 483, 2679
MspA1I	CMGCKG	3 cut(s)	518, 992, 2076
MspI	CCGG	5 cut(s)	771, 1660, 2317, 2440, 2789
MspR9I	CCNGG	6 cut(s)	125, 399, 1247, 2189, 2318, 2580
MunI	CAATTG	1 cut(s)	2166
Mva1269I	GAATGC	1 cut(s)	316
MvaI	CCWGG	5 cut(s)	125, 399, 1247, 2189, 2580
MvnI	CGCG	4 cut(s)	247, 1490, 2767, 2889
NciI	CCSGG	1 cut(s)	2318
NcoI	CCATGG	2 cut(s)	345, 1329
NdeI	CATATG	1 cut(s)	2752
NlaIV	GGNNCC	6 cut(s)	87, 1906, 1907, 2463, 2584, 2838
NmeAIII	GCCGAG	1 cut(s)	373
NmuCI	GTSAC	4 cut(s)	2128, 2203, 2482, 2867
NsiI	ATGCAT	2 cut(s)	750, 1840
NspI	RCATGY	4 cut(s)	748, 1416, 1829, 2451
PaeI	GCATGC	1 cut(s)	2451
PagI	TCATGA	1 cut(s)	1570
PceI	AGGCCT	3 cut(s)	238, 1337, 1798
PciI	ACATGT	1 cut(s)	1412
PctI	GAATGC	1 cut(s)	316
PdmI	GAANNNNTTC	1 cut(s)	647
PfeI	GAWTC	8 cut(s)	128, 563, 681, 917, 1503, 2153, 2718, 2826
PflFI	GACNNNGTC	1 cut(s)	1066
PfoI	TCCNGGA	2 cut(s)	1245, 2316
PleI	GAGTC	3 cut(s)	615, 2908, 2915
PmaCI	CACGTG	1 cut(s)	1709
PmlI	CACGTG	1 cut(s)	1709
PpsI	GAGTC	3 cut(s)	615, 2908, 2915
Ppu21I	YACGTR	1 cut(s)	1709
PpuMI	RGGWCCY	1 cut(s)	1905
PscI	ACATGT	1 cut(s)	1412
PshBI	ATTAAT	2 cut(s)	1641, 1674
Psp124BI	GAGCTC	4 cut(s)	26, 834, 1875, 2430
Psp5II	RGGWCCY	1 cut(s)	1905
Psp6I	CCWGG	5 cut(s)	123, 397, 1245, 2187, 2578
PspCI	CACGTG	1 cut(s)	1709
PspEI	GGTNACC	1 cut(s)	1301
PspFI	CCCAGC	1 cut(s)	2668
PspGI	CCWGG	5 cut(s)	123, 397, 1245, 2187, 2578
PspN4I	GGNNCC	6 cut(s)	87, 1906, 1907, 2463, 2584, 2838
PspPI	GGNCC	3 cut(s)	113, 1905, 2196
PspPPI	RGGWCCY	1 cut(s)	1905
PsrI	GAACNNNNNNTAC	2 cut(s)	830, 862
PstI	CTGCAG	2 cut(s)	2081, 2639
PstNI	CAGNNNCTG	2 cut(s)	766, 1772
PsuI	RGATCY	3 cut(s)	1480, 1942, 2320
PsyI	GACNNNGTC	1 cut(s)	1066
PvuII	CAGCTG	3 cut(s)	518, 992, 2076
RsaI	GTAC	6 cut(s)	557, 614, 848, 2003, 2784, 2884
RsaNI	GTAC	6 cut(s)	556, 613, 847, 2002, 2783, 2883
RseI	CAYNNNNRTG	3 cut(s)	350, 483, 2679
SacI	GAGCTC	4 cut(s)	26, 834, 1875, 2430
SaqAI	TTAA	9 cut(s)	657, 723, 1092, 1403, 1641, 1674, 1761, 1952, 2034
Sau96I	GGNCC	3 cut(s)	113, 1905, 2196
ScaI	AGTACT	1 cut(s)	2003
SchI	GAGTC	3 cut(s)	616, 2909, 2916
ScrFI	CCNGG	6 cut(s)	125, 399, 1247, 2189, 2318, 2580
SduI	GDGCHC	7 cut(s)	26, 834, 1875, 2233, 2430, 2765, 2824
SfaNI	GCATC	9 cut(s)	518, 757, 775, 1063, 1288, 1847, 2056, 2422, 2518
SfcI	CTRYAG	4 cut(s)	303, 1448, 2077, 2635
SinI	GGWCC	2 cut(s)	113, 1905
SmiMI	CAYNNNNRTG	3 cut(s)	350, 483, 2679
SmlI	CTYRAG	2 cut(s)	1602, 2232
SmoI	CTYRAG	2 cut(s)	1602, 2232
SpeI	ACTAGT	1 cut(s)	1192
SphI	GCATGC	1 cut(s)	2451
SseBI	AGGCCT	3 cut(s)	238, 1337, 1798
SsiI	CCGC	4 cut(s)	247, 330, 927, 2307
SspI	AATATT	2 cut(s)	655, 976
SspMI	CTAG	7 cut(s)	240, 1040, 1059, 1193, 1425, 1523, 1794
SstI	GAGCTC	4 cut(s)	26, 834, 1875, 2430
StuI	AGGCCT	3 cut(s)	238, 1337, 1798
StyD4I	CCNGG	6 cut(s)	123, 397, 1245, 2187, 2316, 2578
StyI	CCWWGG	4 cut(s)	345, 1329, 1424, 1556
TaaI	ACNGT	9 cut(s)	259, 1082, 1362, 1456, 1654, 2001, 2056, 2299, 2587
TaiI	ACGT	1 cut(s)	1711
TaqI	TCGA	7 cut(s)	512, 836, 1156, 1533, 1540, 2430, 2824
TaqII	GACCGA	1 cut(s)	1519
TatI	WGTACW	3 cut(s)	555, 612, 2001
TauI	GCSGC	4 cut(s)	250, 333, 930, 2310
TfiI	GAWTC	8 cut(s)	128, 563, 681, 917, 1503, 2153, 2718, 2826
Tru1I	TTAA	9 cut(s)	657, 723, 1092, 1403, 1641, 1674, 1761, 1952, 2034
Tru9I	TTAA	9 cut(s)	657, 723, 1092, 1403, 1641, 1674, 1761, 1952, 2034
TseFI	GTSAC	4 cut(s)	2128, 2203, 2482, 2867
TseI	GCWGC	9 cut(s)	296, 628, 992, 1033, 1719, 2076, 2171, 2550, 2810
Tsp45I	GTSAC	4 cut(s)	2128, 2203, 2482, 2867
TspDTI	ATGAA	6 cut(s)	1224, 1238, 1587, 1640, 1859, 2479
TspGWI	ACGGA	2 cut(s)	1219, 2807
Tth111I	GACNNNGTC	1 cut(s)	1066
VneI	GTGCAC	1 cut(s)	2761
VpaK11BI	GGWCC	2 cut(s)	113, 1905
VspI	ATTAAT	2 cut(s)	1641, 1674
XapI	RAATTY	4 cut(s)	725, 807, 1542, 1757
XceI	RCATGY	4 cut(s)	748, 1416, 1829, 2451
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1253
XmaJI	CCTAGG	1 cut(s)	1424
XmiI	GTMKAC	2 cut(s)	1364, 2556
XmnI	GAANNNNTTC	1 cut(s)	647
XspI	CTAG	7 cut(s)	240, 1040, 1059, 1193, 1425, 1523, 1794
ZrmI	AGTACT	1 cut(s)	2003
Zsp2I	ATGCAT	2 cut(s)	750, 1840

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.