MD13G1221500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007498
Physical Location & Seq
Reverse (-)
21412990 .. 21413909
920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1221500.v1.1.491

Sequence Viewer

Length: 900 bp
ATGAACCAAAACTCGATACCACCACCCCCTTCGCAACCACCATCCAACTCCGACCCCCGCATATTCCACGCTCGTTTCATTACCACCGCAAACGTCGACCGCTCTGTTCTCAACAACCTCATCACTCTATACTCCAAGTCCAATCTCCTCTCTTACTCTCTTCGCCTCTTCCACCAAATCCCATCTCCCAATGTGGTCTCATGGACCGCCGTGATCTCCGCCCACACCAACACCCTCTTCGCCCTCCAGCACTTCGTTTCCATGCTCCGCCACCCCACCTTCCCCAACCAGCGCACCTTCGCTTCCCTCTTCAAAACCTGTGCTTCTCTCCCTTCTCTCTCCTTCGGCCTTGCCCTCCACTCCCTTTCGGTGAAGCTCGGCGTCTCTGCGCAGCCCTTTTCCGGGTCCGCCCTTATTAATTTTTACTGCAAATGCCGCTTGCCTGTTGATGCCCGCAAGGTGCTTGATGAAATTCCTCAGAAAGACGAGGTTTGTTATGGGGCGGTGGTGGTTGGGTTGGCGCAGAACTCGAAACCCATTGATGCGCTGTCTGCTTTTGCTGACATGAAATGTTCTGATGTTAGGTCTACTATGTATAGTGTTTCGGGTGCACTTCGTGCTACAGGGGAGCTTGCTGTGTTGGAGCAATGTAGGATCATACATGCTTATGCTGTTGTTACTGGACTTGATTGGAATGTGATTGTGGCGAGTGCTTTGGTTGATGCGTATGGGAAAGCTGGGCTTGTATCTGATGCTAGACAGGTTTTTGATGAGAATTTTCCGAGCATGAACGTAGTGGGGTGGAATGTGCTGATGGCGGCATACGCACAACAGGGGGATTGGAACTCAACGCTTGAGATTTTCAATGCAATGGAAGCTCAGGGGCTTGCGCCGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

300

Amino Acids

32.57

Weight (kDa)

7.04

Isoelectric Point (pI)

30.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 264 - 298 1.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 390
AccBSI CCGCTC 1 cut(s) 102
AccI GTMKAC 2 cut(s) 96, 587
AclWI GGATC 1 cut(s) 662
AcsI RAATTY 2 cut(s) 471, 775
AcyI GRCGYC 1 cut(s) 381
AdeI CACNNNGTG 1 cut(s) 617
AfiI CCNNNNNNNGG 2 cut(s) 401, 402
AgsI TTSAA 2 cut(s) 313, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 4 cut(s) 376, 631, 737, 878
AluI AGCT 4 cut(s) 376, 631, 737, 878
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 2 cut(s) 202, 388
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 1 cut(s) 662
AoxI GGCC 1 cut(s) 346
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 2 cut(s) 471, 775
AseI ATTAAT 1 cut(s) 417
AspLEI GCGC 5 cut(s) 294, 391, 523, 547, 892
AspS9I GGNCC 2 cut(s) 204, 405
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 2 cut(s) 204, 405
BaeGI GKGCMC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 1 cut(s) 403
BccI CCATC 3 cut(s) 49, 190, 808
BceAI ACGGC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 202, 388
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 3 cut(s) 392, 436, 819
BlsI GCNGC 3 cut(s) 393, 437, 820
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 2 cut(s) 204, 405
BmgT120I GGNCC 2 cut(s) 204, 405
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 4 cut(s) 439, 532, 712, 742
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 402
Bse1I ACTGG 1 cut(s) 685
Bse3DI GCAATG 2 cut(s) 653, 876
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 401, 402
BseMI GCAATG 2 cut(s) 653, 876
BseMII CTCAG 2 cut(s) 491, 893
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 737
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 1 cut(s) 348
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 613
BsiSI CCGG 2 cut(s) 402, 893
BslI CCNNNNNNNGG 2 cut(s) 401, 402
BsmAI GTCTC 2 cut(s) 202, 388
BsmBI CGTCTC 1 cut(s) 388
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 613
Bsp143I GATC 2 cut(s) 213, 654
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 2 cut(s) 490, 892
BspLI GGNNCC 1 cut(s) 406
BspPI GGATC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 202
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 2 cut(s) 653, 876
BsrI ACTGG 1 cut(s) 685
BssMI GATC 2 cut(s) 213, 654
BssNI GRCGYC 1 cut(s) 381
Bst6I CTCTTC 4 cut(s) 165, 173, 242, 314
BstACI GRCGYC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 440, 454, 633, 888
BstDEI CTNAG 2 cut(s) 477, 879
BstF5I GGATG 1 cut(s) 41
BstHHI GCGC 5 cut(s) 294, 391, 523, 547, 892
BstKTI GATC 2 cut(s) 216, 657
BstMAI GTCTC 2 cut(s) 202, 388
BstMBI GATC 2 cut(s) 213, 654
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 5 cut(s) 435, 551, 617, 824, 875
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 621
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 1 cut(s) 403
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 1 cut(s) 41
Cac8I GCNNGC 4 cut(s) 440, 454, 633, 888
CfoI GCGC 5 cut(s) 294, 391, 523, 547, 892
Cfr13I GGNCC 2 cut(s) 204, 405
CseI GACGC 1 cut(s) 370
CviAII CATG 5 cut(s) 201, 262, 565, 662, 787
CviJI RGCY 8 cut(s) 348, 376, 394, 631, 737, 742, 878, 886
CviKI_1 RGCY 8 cut(s) 348, 376, 394, 631, 737, 742, 878, 886
DdeI CTNAG 2 cut(s) 477, 879
DpnI GATC 2 cut(s) 215, 656
DpnII GATC 2 cut(s) 213, 654
DraIII CACNNNGTG 1 cut(s) 617
Eam1104I CTCTTC 4 cut(s) 165, 173, 242, 314
EarI CTCTTC 4 cut(s) 165, 173, 242, 314
EciI GGCGGA 3 cut(s) 208, 257, 397
Eco31I GGTCTC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 204, 405
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 5 cut(s) 204, 265, 568, 665, 790
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FatI CATG 5 cut(s) 200, 261, 564, 661, 786
FauI CCCGC 2 cut(s) 65, 461
FblI GTMKAC 2 cut(s) 96, 587
Fnu4HI GCNGC 3 cut(s) 392, 436, 819
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 3 cut(s) 392, 436, 819
FspBI CTAG 1 cut(s) 756
FspI TGCGCA 1 cut(s) 390
GlaI GCGC 5 cut(s) 293, 390, 522, 546, 891
GluI GCNGC 3 cut(s) 392, 436, 819
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 1 cut(s) 230
HaeIII GGCC 1 cut(s) 348
HapII CCGG 2 cut(s) 402, 893
HgaI GACGC 1 cut(s) 370
HhaI GCGC 5 cut(s) 294, 391, 523, 547, 892
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 5 cut(s) 204, 265, 568, 665, 790
Hin6I GCGC 5 cut(s) 292, 389, 521, 545, 890
HinP1I GCGC 5 cut(s) 292, 389, 521, 545, 890
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HpaII CCGG 2 cut(s) 402, 893
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 3 cut(s) 97, 588, 611
Hpy188I TCNGA 5 cut(s) 52, 480, 577, 751, 783
Hpy8I GTNNAC 3 cut(s) 97, 588, 611
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 5 cut(s) 39, 289, 307, 342, 352
HpyCH4IV ACGT 2 cut(s) 93, 792
HpyCH4V TGCA 3 cut(s) 429, 611, 869
HpyF10VI GCNNNNNNNGC 5 cut(s) 435, 551, 617, 824, 875
HpyF3I CTNAG 2 cut(s) 477, 879
HpySE526I ACGT 2 cut(s) 93, 792
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 5 cut(s) 204, 265, 568, 665, 790
HspAI GCGC 5 cut(s) 292, 389, 521, 545, 890
Kzo9I GATC 2 cut(s) 213, 654
LmnI GCTCC 3 cut(s) 270, 628, 643
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 4 cut(s) 439, 532, 712, 742
MaeI CTAG 1 cut(s) 756
MaeII ACGT 2 cut(s) 93, 792
MaeIII GTNAC 1 cut(s) 676
MalI GATC 2 cut(s) 215, 656
MbiI CCGCTC 1 cut(s) 102
MboI GATC 2 cut(s) 213, 654
MboII GAAGA 4 cut(s) 152, 160, 229, 301
MhlI GDGCHC 1 cut(s) 613
MluCI AATT 3 cut(s) 418, 471, 775
MmeI TCCRAC 3 cut(s) 69, 75, 621
MnlI CCTC 9 cut(s) 128, 158, 176, 245, 254, 317, 365, 481, 486
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 2 cut(s) 402, 893
MspR9I CCNGG 1 cut(s) 403
MwoI GCNNNNNNNGC 5 cut(s) 435, 551, 617, 824, 875
NciI CCSGG 1 cut(s) 403
NdeII GATC 2 cut(s) 213, 654
NlaIII CATG 5 cut(s) 204, 265, 568, 665, 790
NlaIV GGNNCC 1 cut(s) 406
NmeAIII GCCGAG 1 cut(s) 357
NsbI TGCGCA 1 cut(s) 390
NspI RCATGY 1 cut(s) 665
PkrI GCNGC 3 cut(s) 393, 437, 820
PshBI ATTAAT 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 737
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 2 cut(s) 204, 405
RseI CAYNNNNRTG 1 cut(s) 666
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 3 cut(s) 392, 436, 819
Sau3AI GATC 2 cut(s) 213, 654
Sau96I GGNCC 2 cut(s) 204, 405
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 4 cut(s) 439, 532, 712, 742
SfcI CTRYAG 1 cut(s) 621
SinI GGWCC 2 cut(s) 204, 405
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 3 cut(s) 418, 471, 775
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 1 cut(s) 401
TaiI ACGT 2 cut(s) 96, 795
TaqI TCGA 3 cut(s) 14, 96, 530
TasI AATT 3 cut(s) 418, 471, 775
TauI GCSGC 2 cut(s) 438, 821
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseI GCWGC 1 cut(s) 391
TspDTI ATGAA 5 cut(s) 17, 67, 483, 581, 803
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 2 cut(s) 204, 405
VspI ATTAAT 1 cut(s) 417
XapI RAATTY 2 cut(s) 471, 775
XceI RCATGY 1 cut(s) 665
XmiI GTMKAC 2 cut(s) 96, 587
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.