Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006863

Physical Location & Seq

Genomic Coordinates Reverse (-)

22222219 .. 22227638

Gene Structure 5420 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1227100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1386 bp

ATGTGTTCTGTCATTGCCAAGCTCCCTTCGCTTCCACTCTCCCTCTCCATCGTCTCCTCATCTTCTTCTTCCTCCAAGGATGGGAAAACCAACTCAAAGTCGGTGAAGCTGAGAGAGGACTGGCGGCAACGCTCCAGGGCCATCCCACCCGGCGGCACTTACCCGGCTAAAGACCACTGCAGTCGTTGCGGGTTGTGTGACACATATTACATTGCCCATGTCAAGGAAGCTTGTGCTTTCTTAGGAGATGGAATGTCTAAGATTGAAGGTTTGGAACCTGTAGTTCATGGTAGAGGGAGGAAACCGGGTTCCTCAGATGAAATGTACTTGGGTGTCTATGAAGAGCTCTTGTATGCTCGTAAAGTAAAGCCTGTCGAAGGAGCTCAGTGGACAGGAATAGTAACAACTATTGCAATCGAAATGCTGAAATCAGGCATGGTGGAGGCCGTCATTTGTGTACAGAGTGACCCAAACGACAGATTTACTCCTCGACCTGTATTAGCAAGGACACCAGAGGAAGTTCTAGCTGCTAGAGGTGTCAAGCCGACATTATCTCCTAATCTGAATACCCTTGCCCTAGTTGAGGCTGCAGGTGTAAAAAAGCTTCTATTCTGTGGTGTGGGTTGCCAGGTGCAAGCATTACGATCTGTGGAGCACCACTTAAATTTGGACAAGCTCTACGTGCTAGGAACAAATTGCGTGGACAACGGGACTCGAGAAGGCCTTGATAAGTTTCTGAAGGCTGCGAGTAGTGAACCAGAGACAGTTCTTCATTACGAGTTTATGCAAGATTACAAGGTTCACTTGAAGCATTTGGATGGTCATATTGAAGAGGTTCCTTATTTTTCTCTGCCAGCAAATGACTTAGTTGATGTAATTGCTCCTTCTTGTTATAGCTGTTTTGACTATACAAATGGTTTAGCGGATTTAGTTGTTGGATACATGGGGGTGCCGAAATATTCTGGAATCAGCATGACCCAACATCCACAGTATGTTACGGTAAGAAATGAACGTGGAAGAGAGATGCTCAGTCTGATTAAAAAGCATTTGGCGATCACTCCAACAACTAGTAGTGGTACTCGCGGACCATTTACCATGGAGACTGTTAAGGCTGATGATACTGCTAAGATGGGAAAAGGTCCTGAGCCTGCACCAAAGTTTGTCGGCAATGTGATCGCTTTCTTGTTAAACTTGATTGGCCCAAAAGGTTTGGAATTTGCTCGTTATTCGCTCGATTACCATACCATCCGGAACTACCTATATGTAAACCGAACATGGGGAAAACAAAGAGCTGATCAGCATATGCCTTCATATGCAAAGAAGATTGTCGATTCCTACAACAAGAATGGTGAAATCGATCGGATTCTTTCCACCAAGCCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006778 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0015994 GO:0016491 GO:0016725 GO:0033013 GO:0033354 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051186 GO:0052592 GO:0055114 GO:0071704 GO:0090415 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

FrhB_FdhB_C FrhB_FdhB_N

Protein Analysis

462

Amino Acids

50.85

Weight (kDa)

8.76

Isoelectric Point (pI)

33.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FrhB_FdhB_N	PF04422	117 - 191	3.1e-25	Coenzyme F420 hydrogenase/dehydrogenase, beta subunit N-term
FrhB_FdhB_C	PF04432	200 - 350	2.9e-26	Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04620
fragaria_vesca	FvH4_4g05310 FvH4_4g05310 FvH4_4g05310 FvH4_4g05310
malus_domestica	MD13G1227100.v1.1
prunus_persica	Prupe.1G054400_v2.0.a1 Prupe.1G054600_v2.0.a1 Prupe.1G054600_v2.0.a1 Prupe.1G054600_v2.0.a1 Prupe.1G054600_v2.0.a1 Prupe.1G054600_v2.0.a1 Prupe.1G054600_v2.0.a1 Prupe.1G054600_v2.0.a1
pyrus_communis	pycom13g20010
rosa_chinensis	RchiOBHm_Chr4g0396281
rosa_laevigata	RLG00000009554
rosa_multiflora	Rmu_sc0002578.1_g000007 Rmu_ssc0000234.1_g000008
rosa_roxburghii	Rroxscaffold_5G00341120
rosa_rugosa	Rorug03G0350500 Rorug03G0350600.1
rosa_samantha	Rh4BG066300 Rh4CG073100 Rh4DG063100
rosa_wichuraiana	Rw4G005450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	581
Acc36I	ACCTGC	1 cut(s)	581
AccB1I	GGYRCC	1 cut(s)	949
AccII	CGCG	1 cut(s)	1083
AccIII	TCCGGA	1 cut(s)	1248
AciI	CCGC	5 cut(s)	124, 153, 189, 923, 1083
AcsI	RAATTY	2 cut(s)	664, 1214
AcuI	CTGAAG	1 cut(s)	758
AfaI	GTAC	3 cut(s)	326, 459, 1078
AfiI	CCNNNNNNNGG	6 cut(s)	81, 152, 223, 377, 583, 1276
AgsI	TTSAA	3 cut(s)	266, 808, 830
AhlI	ACTAGT	1 cut(s)	1067
AjnI	CCWGG	2 cut(s)	134, 627
AloI	GAACNNNNNNTCC	2 cut(s)	292, 324
Alw21I	GWGCWC	3 cut(s)	348, 385, 657
Alw26I	GTCTC	3 cut(s)	58, 755, 1094
Ama87I	CYCGRG	1 cut(s)	714
Aor13HI	TCCGGA	1 cut(s)	1248
AoxI	GGCC	4 cut(s)	138, 444, 721, 1198
ApeKI	GCWGC	3 cut(s)	527, 587, 743
ApoI	RAATTY	2 cut(s)	664, 1214
Asp700I	GAANNNNTTC	1 cut(s)	834
AspS9I	GGNCC	4 cut(s)	138, 1085, 1139, 1199
AsuC2I	CCSGG	3 cut(s)	150, 164, 306
AsuHPI	GGTGA	2 cut(s)	115, 1361
AvaI	CYCGRG	1 cut(s)	714
AvaII	GGWCC	2 cut(s)	1085, 1139
BanI	GGYRCC	1 cut(s)	949
BanII	GRGCYC	2 cut(s)	348, 385
BarI	GAAGNNNNNNTAC	2 cut(s)	588, 620
Bbv12I	GWGCWC	3 cut(s)	348, 385, 657
BbvI	GCAGC	3 cut(s)	514, 574, 730
BccI	CCATC	7 cut(s)	56, 74, 149, 242, 812, 1123, 1253
BceAI	ACGGC	1 cut(s)	431
BcgI	CGANNNNNNTGC	2 cut(s)	1156, 1190
BciT130I	CCWGG	2 cut(s)	136, 629
BciVI	GTATCC	1 cut(s)	932
BclI	TGATCA	1 cut(s)	1294
BcnI	CCSGG	3 cut(s)	150, 164, 306
BcoDI	GTCTC	3 cut(s)	58, 755, 1094
BcuI	ACTAGT	1 cut(s)	1067
BfaI	CTAG	5 cut(s)	524, 531, 578, 686, 1068
BfmI	CTRYAG	3 cut(s)	178, 279, 588
BfuAI	ACCTGC	1 cut(s)	581
BfuI	GTATCC	1 cut(s)	932
BisI	GCNGC	5 cut(s)	125, 154, 528, 588, 744
BlsI	GCNGC	5 cut(s)	126, 155, 529, 589, 745
Bme1390I	CCNGG	5 cut(s)	136, 150, 164, 306, 629
Bme18I	GGWCC	2 cut(s)	1085, 1139
BmeT110I	CYCGRG	1 cut(s)	714
BmgT120I	GGNCC	4 cut(s)	138, 1085, 1139, 1199
BmiI	GGNNCC	4 cut(s)	276, 310, 837, 951
BmrFI	CCNGG	5 cut(s)	136, 150, 164, 306, 629
BmsI	GCATC	1 cut(s)	1014
BplI	GAGNNNNNCTC	2 cut(s)	1011, 1043
BpmI	CTGGAG	1 cut(s)	118
Bpu10I	CCTNAGC	1 cut(s)	1143
BpuMI	CCSGG	3 cut(s)	150, 164, 306
Bsa29I	ATCGAT	1 cut(s)	1356
BsaAI	YACGTR	1 cut(s)	682
BsaJI	CCNNGG	3 cut(s)	75, 135, 1095
BsaWI	WCCGGW	1 cut(s)	1248
BsaXI	ACNNNNNCTCC	2 cut(s)	538, 568
Bsc4I	CCNNNNNNNGG	6 cut(s)	81, 152, 223, 377, 583, 1276
Bse1I	ACTGG	1 cut(s)	125
Bse3DI	GCAATG	3 cut(s)	12, 210, 1174
BseAI	TCCGGA	1 cut(s)	1248
BseBI	CCWGG	2 cut(s)	136, 629
BseCI	ATCGAT	1 cut(s)	1356
BseDI	CCNNGG	3 cut(s)	75, 135, 1095
BseGI	GGATG	5 cut(s)	85, 141, 823, 982, 1245
BseLI	CCNNNNNNNGG	6 cut(s)	81, 152, 223, 377, 583, 1276
BseMI	GCAATG	3 cut(s)	12, 210, 1174
BseMII	CTCAG	5 cut(s)	101, 327, 398, 1042, 1134
BseNI	ACTGG	1 cut(s)	125
BseRI	GAGGAG	2 cut(s)	46, 477
BseXI	GCAGC	3 cut(s)	514, 574, 730
BsgI	GTGCAG	1 cut(s)	1134
Bsh1236I	CGCG	1 cut(s)	1083
Bsh1285I	CGRYCG	1 cut(s)	1360
BshFI	GGCC	4 cut(s)	140, 446, 723, 1200
BshNI	GGYRCC	1 cut(s)	949
BshVI	ATCGAT	1 cut(s)	1356
BsiEI	CGRYCG	1 cut(s)	1360
BsiHKAI	GWGCWC	3 cut(s)	348, 385, 657
BsiHKCI	CYCGRG	1 cut(s)	714
BsiSI	CCGG	4 cut(s)	150, 164, 305, 1249
BslFI	GGGAC	1 cut(s)	724
BslI	CCNNNNNNNGG	6 cut(s)	81, 152, 223, 377, 583, 1276
BsmAI	GTCTC	3 cut(s)	58, 755, 1094
BsmBI	CGTCTC	1 cut(s)	58
BsmFI	GGGAC	1 cut(s)	724
BsnI	GGCC	4 cut(s)	140, 446, 723, 1200
BsoBI	CYCGRG	1 cut(s)	714
Bsp1286I	GDGCHC	3 cut(s)	348, 385, 657
Bsp13I	TCCGGA	1 cut(s)	1248
Bsp1407I	TGTACA	1 cut(s)	457
Bsp143I	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
Bsp19I	CCATGG	1 cut(s)	1095
BspACI	CCGC	5 cut(s)	124, 153, 189, 923, 1083
BspANI	GGCC	4 cut(s)	140, 446, 723, 1200
BspCNI	CTCAG	5 cut(s)	102, 326, 397, 1041, 1135
BspDI	ATCGAT	1 cut(s)	1356
BspEI	TCCGGA	1 cut(s)	1248
BspFNI	CGCG	1 cut(s)	1083
BspLI	GGNNCC	4 cut(s)	276, 310, 837, 951
BspMAI	CTGCAG	2 cut(s)	182, 592
BspMI	ACCTGC	1 cut(s)	581
BspQI	GCTCTTC	1 cut(s)	336
BspT107I	GGYRCC	1 cut(s)	949
BsrDI	GCAATG	3 cut(s)	12, 210, 1174
BsrGI	TGTACA	1 cut(s)	457
BsrI	ACTGG	1 cut(s)	125
BssECI	CCNNGG	3 cut(s)	75, 135, 1095
BssMI	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
BssT1I	CCWWGG	2 cut(s)	75, 1095
Bst2UI	CCWGG	2 cut(s)	136, 629
Bst4CI	ACNGT	4 cut(s)	766, 990, 1000, 1105
Bst6I	CTCTTC	3 cut(s)	336, 825, 1012
BstAPI	GCANNNNNTGC	1 cut(s)	186
BstAUI	TGTACA	1 cut(s)	457
BstBAI	YACGTR	1 cut(s)	682
BstC8I	GCNNGC	3 cut(s)	636, 855, 1149
BstDEI	CTNAG	9 cut(s)	110, 241, 258, 313, 384, 865, 1028, 1125, 1143
BstDSI	CCRYGG	1 cut(s)	1095
BstENI	CCTNNNNNAGG	2 cut(s)	375, 581
BstF5I	GGATG	5 cut(s)	85, 141, 823, 982, 1245
BstFNI	CGCG	1 cut(s)	1083
BstKTI	GATC	5 cut(s)	647, 1056, 1176, 1297, 1360
BstMAI	GTCTC	3 cut(s)	58, 755, 1094
BstMBI	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
BstMCI	CGRYCG	1 cut(s)	1360
BstMWI	GCNNNNNNNGC	3 cut(s)	28, 186, 682
BstNI	CCWGG	2 cut(s)	136, 629
BstSCI	CCNGG	5 cut(s)	134, 148, 162, 304, 627
BstSFI	CTRYAG	3 cut(s)	178, 279, 588
BstUI	CGCG	1 cut(s)	1083
BstV1I	GCAGC	3 cut(s)	514, 574, 730
Bsu15I	ATCGAT	1 cut(s)	1356
BsuI	GTATCC	1 cut(s)	932
BsuRI	GGCC	4 cut(s)	140, 446, 723, 1200
BsuTUI	ATCGAT	1 cut(s)	1356
BtgI	CCRYGG	1 cut(s)	1095
BtsCI	GGATG	5 cut(s)	85, 141, 823, 982, 1245
BtsI	GCAGTG	1 cut(s)	175
BtsIMutI	CAGTG	2 cut(s)	175, 392
BveI	ACCTGC	1 cut(s)	581
Cac8I	GCNNGC	3 cut(s)	636, 855, 1149
Cfr13I	GGNCC	4 cut(s)	138, 1085, 1139, 1199
ClaI	ATCGAT	1 cut(s)	1356
Csp6I	GTAC	3 cut(s)	325, 458, 1077
CspCI	CAANNNNNGTGG	2 cut(s)	681, 716
CviAII	CATG	7 cut(s)	218, 287, 436, 943, 973, 1096, 1275
CviQI	GTAC	3 cut(s)	325, 458, 1077
DdeI	CTNAG	9 cut(s)	110, 241, 258, 313, 384, 865, 1028, 1125, 1143
DpnI	GATC	5 cut(s)	646, 1055, 1175, 1296, 1359
DpnII	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
Eam1104I	CTCTTC	3 cut(s)	336, 825, 1012
EarI	CTCTTC	3 cut(s)	336, 825, 1012
Ecl136II	GAGCTC	2 cut(s)	346, 383
Eco130I	CCWWGG	2 cut(s)	75, 1095
Eco147I	AGGCCT	1 cut(s)	723
Eco24I	GRGCYC	2 cut(s)	348, 385
Eco47I	GGWCC	2 cut(s)	1085, 1139
Eco53kI	GAGCTC	2 cut(s)	346, 383
Eco57I	CTGAAG	1 cut(s)	758
Eco88I	CYCGRG	1 cut(s)	714
EcoICRI	GAGCTC	2 cut(s)	346, 383
EcoNI	CCTNNNNNAGG	2 cut(s)	375, 581
EcoO109I	RGGNCCY	1 cut(s)	1139
EcoRII	CCWGG	2 cut(s)	134, 627
EcoT14I	CCWWGG	2 cut(s)	75, 1095
EcoT38I	GRGCYC	2 cut(s)	348, 385
ErhI	CCWWGG	2 cut(s)	75, 1095
Esp3I	CGTCTC	1 cut(s)	58
FaeI	CATG	7 cut(s)	221, 290, 439, 946, 976, 1099, 1278
FalI	AAGNNNNNCTT	2 cut(s)	788, 820
FaqI	GGGAC	1 cut(s)	724
FatI	CATG	7 cut(s)	217, 286, 435, 942, 972, 1095, 1274
FauI	CCCGC	1 cut(s)	182
FauNDI	CATATG	2 cut(s)	1302, 1312
FbaI	TGATCA	1 cut(s)	1294
Fnu4HI	GCNGC	5 cut(s)	125, 154, 528, 588, 744
FokI	GGATG	5 cut(s)	92, 128, 830, 969, 1232
FriOI	GRGCYC	2 cut(s)	348, 385
Fsp4HI	GCNGC	5 cut(s)	125, 154, 528, 588, 744
FspBI	CTAG	5 cut(s)	524, 531, 578, 686, 1068
GluI	GCNGC	5 cut(s)	125, 154, 528, 588, 744
GsuI	CTGGAG	1 cut(s)	118
HaeIII	GGCC	4 cut(s)	140, 446, 723, 1200
HapII	CCGG	4 cut(s)	150, 164, 305, 1249
Hin1II	CATG	7 cut(s)	221, 290, 439, 946, 976, 1099, 1278
HindIII	AAGCTT	2 cut(s)	228, 602
HinfI	GANTC	4 cut(s)	712, 966, 1331, 1363
HpaII	CCGG	4 cut(s)	150, 164, 305, 1249
HphI	GGTGA	2 cut(s)	115, 1361
Hpy166II	GTNNAC	6 cut(s)	390, 458, 703, 755, 802, 1267
Hpy188I	TCNGA	5 cut(s)	316, 564, 738, 1035, 1362
Hpy188III	TCNNGA	4 cut(s)	716, 963, 1142, 1249
Hpy8I	GTNNAC	6 cut(s)	390, 458, 703, 755, 802, 1267
HpyAV	CCTTC	7 cut(s)	36, 260, 371, 713, 733, 894, 1317
HpyCH4III	ACNGT	4 cut(s)	766, 990, 1000, 1105
HpyCH4IV	ACGT	2 cut(s)	681, 1012
HpyCH4V	TGCA	7 cut(s)	180, 413, 590, 634, 787, 1151, 1316
HpyF10VI	GCNNNNNNNGC	3 cut(s)	28, 186, 682
HpyF3I	CTNAG	9 cut(s)	110, 241, 258, 313, 384, 865, 1028, 1125, 1143
HpySE526I	ACGT	2 cut(s)	681, 1012
Hsp92II	CATG	7 cut(s)	221, 290, 439, 946, 976, 1099, 1278
Kpn2I	TCCGGA	1 cut(s)	1248
Ksp22I	TGATCA	1 cut(s)	1294
Kzo9I	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
LguI	GCTCTTC	1 cut(s)	336
LmnI	GCTCC	5 cut(s)	27, 137, 380, 652, 886
Lsp1109I	GCAGC	3 cut(s)	514, 574, 730
LweI	GCATC	1 cut(s)	1014
MaeI	CTAG	5 cut(s)	524, 531, 578, 686, 1068
MaeII	ACGT	2 cut(s)	681, 1012
MaeIII	GTNAC	4 cut(s)	197, 400, 464, 994
MalI	GATC	5 cut(s)	646, 1055, 1175, 1296, 1359
MboI	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
MboII	GAAGA	8 cut(s)	54, 57, 60, 353, 761, 842, 1029, 1333
MhlI	GDGCHC	3 cut(s)	348, 385, 657
MluCI	AATT	4 cut(s)	664, 694, 876, 1214
MlyI	GAGTC	1 cut(s)	706
MmeI	TCCRAC	2 cut(s)	916, 1085
MroI	TCCGGA	1 cut(s)	1248
MroXI	GAANNNNTTC	1 cut(s)	834
MseI	TTAA	4 cut(s)	662, 1038, 1107, 1187
MslI	CAYNNNNRTG	2 cut(s)	816, 947
MspI	CCGG	4 cut(s)	150, 164, 305, 1249
MspR9I	CCNGG	5 cut(s)	136, 150, 164, 306, 629
MvaI	CCWGG	2 cut(s)	136, 629
MvnI	CGCG	1 cut(s)	1083
MwoI	GCNNNNNNNGC	3 cut(s)	28, 186, 682
NciI	CCSGG	3 cut(s)	150, 164, 306
NcoI	CCATGG	1 cut(s)	1095
NdeI	CATATG	2 cut(s)	1302, 1312
NdeII	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
NlaIII	CATG	7 cut(s)	221, 290, 439, 946, 976, 1099, 1278
NlaIV	GGNNCC	4 cut(s)	276, 310, 837, 951
NmuCI	GTSAC	2 cut(s)	197, 464
PaeR7I	CTCGAG	1 cut(s)	714
PaqCI	CACCTGC	1 cut(s)	581
PceI	AGGCCT	1 cut(s)	723
PciSI	GCTCTTC	1 cut(s)	336
PdmI	GAANNNNTTC	1 cut(s)	834
PfeI	GAWTC	3 cut(s)	966, 1331, 1363
PkrI	GCNGC	5 cut(s)	126, 155, 529, 589, 745
Ple19I	CGATCG	1 cut(s)	1360
PleI	GAGTC	1 cut(s)	706
PpsI	GAGTC	1 cut(s)	706
Ppu21I	YACGTR	1 cut(s)	682
PpuMI	RGGWCCY	1 cut(s)	1139
Psp124BI	GAGCTC	2 cut(s)	348, 385
Psp5II	RGGWCCY	1 cut(s)	1139
Psp6I	CCWGG	2 cut(s)	134, 627
PspGI	CCWGG	2 cut(s)	134, 627
PspN4I	GGNNCC	4 cut(s)	276, 310, 837, 951
PspPI	GGNCC	4 cut(s)	138, 1085, 1139, 1199
PspPPI	RGGWCCY	1 cut(s)	1139
PstI	CTGCAG	2 cut(s)	182, 592
PvuI	CGATCG	1 cut(s)	1360
RsaI	GTAC	3 cut(s)	326, 459, 1078
RsaNI	GTAC	3 cut(s)	325, 458, 1077
RseI	CAYNNNNRTG	2 cut(s)	816, 947
SacI	GAGCTC	2 cut(s)	348, 385
SapI	GCTCTTC	1 cut(s)	336
SaqAI	TTAA	4 cut(s)	662, 1038, 1107, 1187
SatI	GCNGC	5 cut(s)	125, 154, 528, 588, 744
Sau3AI	GATC	5 cut(s)	644, 1053, 1173, 1294, 1357
Sau96I	GGNCC	4 cut(s)	138, 1085, 1139, 1199
SchI	GAGTC	1 cut(s)	706
ScrFI	CCNGG	5 cut(s)	136, 150, 164, 306, 629
SduI	GDGCHC	3 cut(s)	348, 385, 657
SfaNI	GCATC	1 cut(s)	1014
SfcI	CTRYAG	3 cut(s)	178, 279, 588
Sfr274I	CTCGAG	1 cut(s)	714
SinI	GGWCC	2 cut(s)	1085, 1139
SlaI	CTCGAG	1 cut(s)	714
SmiMI	CAYNNNNRTG	2 cut(s)	816, 947
SmlI	CTYRAG	1 cut(s)	714
SmoI	CTYRAG	1 cut(s)	714
SpeI	ACTAGT	1 cut(s)	1067
Sse9I	AATT	4 cut(s)	664, 694, 876, 1214
SseBI	AGGCCT	1 cut(s)	723
SsiI	CCGC	5 cut(s)	124, 153, 189, 923, 1083
SspI	AATATT	1 cut(s)	959
SspMI	CTAG	5 cut(s)	524, 531, 578, 686, 1068
SstI	GAGCTC	2 cut(s)	348, 385
StuI	AGGCCT	1 cut(s)	723
StyD4I	CCNGG	5 cut(s)	134, 148, 162, 304, 627
StyI	CCWWGG	2 cut(s)	75, 1095
TaaI	ACNGT	4 cut(s)	766, 990, 1000, 1105
TaiI	ACGT	2 cut(s)	684, 1015
TaqI	TCGA	7 cut(s)	375, 417, 490, 715, 1233, 1329, 1356
TasI	AATT	4 cut(s)	664, 694, 876, 1214
TatI	WGTACW	2 cut(s)	324, 457
TauI	GCSGC	2 cut(s)	127, 156
TfiI	GAWTC	3 cut(s)	966, 1331, 1363
Tru1I	TTAA	4 cut(s)	662, 1038, 1107, 1187
Tru9I	TTAA	4 cut(s)	662, 1038, 1107, 1187
TscAI	CASTG	2 cut(s)	182, 392
TseFI	GTSAC	2 cut(s)	197, 464
TseI	GCWGC	3 cut(s)	527, 587, 743
Tsp45I	GTSAC	2 cut(s)	197, 464
TspDTI	ATGAA	6 cut(s)	275, 333, 354, 761, 1023, 1299
TspRI	CASTG	2 cut(s)	182, 392
VpaK11BI	GGWCC	2 cut(s)	1085, 1139
XagI	CCTNNNNNAGG	2 cut(s)	375, 581
XapI	RAATTY	2 cut(s)	664, 1214
XhoI	CTCGAG	1 cut(s)	714
XmnI	GAANNNNTTC	1 cut(s)	834
XspI	CTAG	5 cut(s)	524, 531, 578, 686, 1068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.