Prupe.1G054600_v2.0.a1

7-hydroxymethyl chlorophyll a reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0006863
Physical Location & Seq
Reverse (-)
3841111 .. 3848333
7223 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G054600.7

Sequence Viewer

Length: 1404 bp
ATGTGTTCTCTAATTGCCAAGCTCTATTCCCTTCCTCTCTCACTCTCCATTGTTTCCTCATCTTCATCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTAAAGATGGAAAACCCAACTCAAAGTCAGTGAAGCTGAGAGAGGACTGGAGGCAACGCTCCAGACCCATTCCTCCTGGTGGCACTTATCCGGCTAAAGACCACTGCAGTCGTTGCGGGTTGTGCGACACATATTACATTGCCCATGTCAAGGAAGCCTGTGCTTTCTTAGGAGATGGAATGTCTAAGATTGAAGGCTTGGAACCTGTAGTTCATGGTAGAGGGAGAAAAACAGGTTCCTCAGATGAAATGTACTTGGGTGTCTATGAGGAGTTGTTGTATGCTCGTAAAGTGATCCCTGTTGAAGGAGCTCAGTGGACAGGAATAGTAACAACTATTGCAATAGAAATGCTAAAATCAGGCATGGTAGAGGCCGTAATTTGTGTACAGAGTGACCCAAACGACAGATTTACTGCACGGCCTGTATTAGCAAGGACACCAGAGGAAGTTTTAGCTGCTAAAGGTGTCAAGCCGACATTATCTCCTAATCTTAATACCCTTGCCCTAGTTGAGGCTGCAGGTGTAAAGAAACTTCTATTCTGTGGTGTGGGTTGCCAGGTGCAAGCATTACGATCTGTGGAGCACCATTTAAATTTGGACAAGCTCTATGTGTTAGGCACAAATTGCGTGGACAATGGGACTCGAGAAGGGCTTGATAAGTTTTTAAAGGCTGCAAGTAGTGAACCGGAGACAGTTCTTCATTATGAGTTTATGCAAGATTACAAGGTTCACTTGAAGCATTTGGATGGTCATATTGAAGAGGTTCCGTATTTCTCTCTGCCAGCAAATGAGTTGGTTGATGTAATTGCTCCTTCTTGTTATAGTTGTTTCGATTATACAAATGGTTTAGCGGATTTAGTTGTGGGATACATGGGTGTGCCAAAATATTCTGGAATCAGCATGACTCAACATCCACAGTATGTTACGGTAAGAAATGAACGTGGAAGAGAGATGCTCAGTCTGGTTGATAATCTTTTGGAGGTTACTCCAACAACTAGCAGTGGAAATCGCCAACCATTTGTCATGGAGACTGTTAAGGCAGATGATAATGCAAAGTTGGGAAAGGGTCCTGAACCTGCCCCAAAGTTTGTTGGCAATGTGATTGCTTTCTTGCTAAACTTGATTGGCCCAAAGGGTCTGGAATTTGCTCGTTATTCACTCGATTACCATACCATCAGGAACTACCTGTATGTAAACCGAATATGGGGAAAACAAAGAGCTGATCAGCATATGCCTTCATATGCAAAGAAGATTGTGGATACCTACAATAAGAATGGTGAAATCGATCGGATTCTTACCAACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 map00860 map01100 map01110
Pfam Domains
Protein Families
FrhB_FdhB_C FrhB_FdhB_N

Protein Analysis

468

Amino Acids

51.48

Weight (kDa)

7.91

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 605
Acc36I ACCTGC 2 cut(s) 605, 1180
AciI CCGC 2 cut(s) 213, 947
AclWI GGATC 1 cut(s) 385
AcsI RAATTY 2 cut(s) 688, 1238
AfaI GTAC 2 cut(s) 350, 483
AfiI CCNNNNNNNGG 5 cut(s) 176, 247, 401, 607, 1300
AgsI TTSAA 4 cut(s) 290, 401, 832, 854
AjnI CCWGG 2 cut(s) 172, 651
AluBI AGCT 6 cut(s) 22, 133, 407, 551, 700, 1316
AluI AGCT 6 cut(s) 22, 133, 407, 551, 700, 1316
Alw21I GWGCWC 2 cut(s) 409, 681
Alw26I GTCTC 2 cut(s) 779, 1118
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 738
AoxI GGCC 3 cut(s) 468, 515, 1222
ApeKI GCWGC 3 cut(s) 551, 611, 767
ApoI RAATTY 2 cut(s) 688, 1238
Asp700I GAANNNNTTC 1 cut(s) 858
AspS9I GGNCC 2 cut(s) 1163, 1223
AsuHPI GGTGA 1 cut(s) 1385
AvaI CYCGRG 1 cut(s) 738
AvaII GGWCC 1 cut(s) 1163
BaeI ACNNNNGTAYC 2 cut(s) 955, 988
BanII GRGCYC 1 cut(s) 409
BarI GAAGNNNNNNTAC 2 cut(s) 612, 644
Bbv12I GWGCWC 2 cut(s) 409, 681
BbvI GCAGC 3 cut(s) 538, 598, 754
BccI CCATC 4 cut(s) 98, 266, 836, 1277
BceAI ACGGC 2 cut(s) 455, 530
BciT130I CCWGG 2 cut(s) 174, 653
BciVI GTATCC 2 cut(s) 956, 1348
BclI TGATCA 1 cut(s) 1318
BcoDI GTCTC 2 cut(s) 779, 1118
BfaI CTAG 2 cut(s) 602, 1092
BfmI CTRYAG 3 cut(s) 202, 303, 612
BfuAI ACCTGC 2 cut(s) 605, 1180
BfuI GTATCC 2 cut(s) 956, 1348
BisI GCNGC 3 cut(s) 552, 612, 768
BlsI GCNGC 3 cut(s) 553, 613, 769
Bme1390I CCNGG 2 cut(s) 174, 653
Bme18I GGWCC 1 cut(s) 1163
BmeT110I CYCGRG 1 cut(s) 738
BmgT120I GGNCC 2 cut(s) 1163, 1223
BmiI GGNNCC 4 cut(s) 300, 334, 861, 1164
BmrFI CCNGG 2 cut(s) 174, 653
BmsI GCATC 1 cut(s) 1038
BplI GAGNNNNNCTC 2 cut(s) 1035, 1067
BpmI CTGGAG 2 cut(s) 142, 166
Bsa29I ATCGAT 1 cut(s) 1380
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 562, 592
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 247, 401, 607, 1300
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 2 cut(s) 234, 1198
BseBI CCWGG 2 cut(s) 174, 653
BseCI ATCGAT 1 cut(s) 1380
BseGI GGATG 2 cut(s) 847, 1006
BseLI CCNNNNNNNGG 5 cut(s) 176, 247, 401, 607, 1300
BseMI GCAATG 2 cut(s) 234, 1198
BseMII CTCAG 4 cut(s) 125, 351, 422, 1066
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 3 cut(s) 538, 598, 754
BsgI GTGCAG 1 cut(s) 495
Bsh1285I CGRYCG 1 cut(s) 1384
BshFI GGCC 3 cut(s) 470, 517, 1224
BshVI ATCGAT 1 cut(s) 1380
BsiEI CGRYCG 1 cut(s) 1384
BsiHKAI GWGCWC 2 cut(s) 409, 681
BsiHKCI CYCGRG 1 cut(s) 738
BsiSI CCGG 2 cut(s) 188, 782
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 5 cut(s) 176, 247, 401, 607, 1300
BsmAI GTCTC 2 cut(s) 779, 1118
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 3 cut(s) 470, 517, 1224
BsoBI CYCGRG 1 cut(s) 738
Bsp1286I GDGCHC 2 cut(s) 409, 681
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 4 cut(s) 390, 668, 1318, 1381
BspACI CCGC 2 cut(s) 213, 947
BspANI GGCC 3 cut(s) 470, 517, 1224
BspCNI CTCAG 4 cut(s) 126, 350, 421, 1065
BspDI ATCGAT 1 cut(s) 1380
BspLI GGNNCC 4 cut(s) 300, 334, 861, 1164
BspMAI CTGCAG 2 cut(s) 206, 616
BspMI ACCTGC 2 cut(s) 605, 1180
BspPI GGATC 1 cut(s) 385
BsrDI GCAATG 2 cut(s) 234, 1198
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 1 cut(s) 149
BssMI GATC 4 cut(s) 390, 668, 1318, 1381
Bst2UI CCWGG 2 cut(s) 174, 653
Bst4CI ACNGT 4 cut(s) 790, 1014, 1024, 1129
Bst6I CTCTTC 6 cut(s) 79, 85, 91, 97, 849, 1036
BstAPI GCANNNNNTGC 2 cut(s) 210, 720
BstAUI TGTACA 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 660, 879
BstDEI CTNAG 6 cut(s) 134, 265, 282, 337, 408, 1052
BstENI CCTNNNNNAGG 2 cut(s) 399, 605
BstF5I GGATG 2 cut(s) 847, 1006
BstKTI GATC 4 cut(s) 393, 671, 1321, 1384
BstMAI GTCTC 2 cut(s) 779, 1118
BstMBI GATC 4 cut(s) 390, 668, 1318, 1381
BstMCI CGRYCG 1 cut(s) 1384
BstMWI GCNNNNNNNGC 3 cut(s) 210, 219, 720
BstNI CCWGG 2 cut(s) 174, 653
BstSCI CCNGG 2 cut(s) 172, 651
BstSFI CTRYAG 3 cut(s) 202, 303, 612
BstV1I GCAGC 3 cut(s) 538, 598, 754
Bsu15I ATCGAT 1 cut(s) 1380
BsuI GTATCC 2 cut(s) 956, 1348
BsuRI GGCC 3 cut(s) 470, 517, 1224
BsuTUI ATCGAT 1 cut(s) 1380
BtsCI GGATG 2 cut(s) 847, 1006
BtsI GCAGTG 2 cut(s) 199, 1102
BtsIMutI CAGTG 4 cut(s) 132, 199, 416, 1102
BveI ACCTGC 2 cut(s) 605, 1180
Cac8I GCNNGC 2 cut(s) 660, 879
Cfr13I GGNCC 2 cut(s) 1163, 1223
ClaI ATCGAT 1 cut(s) 1380
Csp6I GTAC 2 cut(s) 349, 482
CspCI CAANNNNNGTGG 2 cut(s) 705, 740
CviAII CATG 6 cut(s) 242, 311, 460, 967, 997, 1120
CviQI GTAC 2 cut(s) 349, 482
DdeI CTNAG 6 cut(s) 134, 265, 282, 337, 408, 1052
DpnI GATC 4 cut(s) 392, 670, 1320, 1383
DpnII GATC 4 cut(s) 390, 668, 1318, 1381
DraI TTTAAA 2 cut(s) 687, 762
Eam1104I CTCTTC 6 cut(s) 79, 85, 91, 97, 849, 1036
EarI CTCTTC 6 cut(s) 79, 85, 91, 97, 849, 1036
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 1163
Eco53kI GAGCTC 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 738
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 2 cut(s) 399, 605
EcoO109I RGGNCCY 1 cut(s) 1163
EcoRII CCWGG 2 cut(s) 172, 651
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 6 cut(s) 245, 314, 463, 970, 1000, 1123
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 1 cut(s) 748
FatI CATG 6 cut(s) 241, 310, 459, 966, 996, 1119
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 2 cut(s) 1326, 1336
FbaI TGATCA 1 cut(s) 1318
Fnu4HI GCNGC 3 cut(s) 552, 612, 768
FokI GGATG 2 cut(s) 854, 993
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 3 cut(s) 552, 612, 768
FspBI CTAG 2 cut(s) 602, 1092
GluI GCNGC 3 cut(s) 552, 612, 768
GsuI CTGGAG 2 cut(s) 142, 166
HaeIII GGCC 3 cut(s) 470, 517, 1224
HapII CCGG 2 cut(s) 188, 782
Hin1II CATG 6 cut(s) 245, 314, 463, 970, 1000, 1123
HinfI GANTC 4 cut(s) 736, 990, 1000, 1387
HpaII CCGG 2 cut(s) 188, 782
HphI GGTGA 1 cut(s) 1385
Hpy166II GTNNAC 6 cut(s) 414, 482, 727, 779, 826, 1291
Hpy188I TCNGA 2 cut(s) 340, 1386
Hpy188III TCNNGA 6 cut(s) 159, 740, 987, 1166, 1235, 1273
Hpy8I GTNNAC 6 cut(s) 414, 482, 727, 779, 826, 1291
HpyAV CCTTC 6 cut(s) 41, 284, 395, 737, 918, 1341
HpyCH4III ACNGT 4 cut(s) 790, 1014, 1024, 1129
HpyCH4IV ACGT 1 cut(s) 1036
HpyCH4V TGCA 9 cut(s) 204, 437, 512, 614, 658, 770, 811, 1148, 1340
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 219, 720
HpyF3I CTNAG 6 cut(s) 134, 265, 282, 337, 408, 1052
HpySE526I ACGT 1 cut(s) 1036
Hsp92II CATG 6 cut(s) 245, 314, 463, 970, 1000, 1123
Ksp22I TGATCA 1 cut(s) 1318
Kzo9I GATC 4 cut(s) 390, 668, 1318, 1381
LmnI GCTCC 4 cut(s) 161, 404, 676, 910
Lsp1109I GCAGC 3 cut(s) 538, 598, 754
LweI GCATC 1 cut(s) 1038
MaeI CTAG 2 cut(s) 602, 1092
MaeII ACGT 1 cut(s) 1036
MaeIII GTNAC 4 cut(s) 424, 488, 1018, 1078
MalI GATC 4 cut(s) 392, 670, 1320, 1383
MboI GATC 4 cut(s) 390, 668, 1318, 1381
MhlI GDGCHC 2 cut(s) 409, 681
MluCI AATT 6 cut(s) 12, 474, 688, 718, 900, 1238
MlyI GAGTC 2 cut(s) 730, 994
MmeI TCCRAC 1 cut(s) 1109
MroXI GAANNNNTTC 1 cut(s) 858
MseI TTAA 4 cut(s) 588, 686, 761, 1131
MslI CAYNNNNRTG 2 cut(s) 840, 971
MspI CCGG 2 cut(s) 188, 782
MspR9I CCNGG 2 cut(s) 174, 653
MvaI CCWGG 2 cut(s) 174, 653
MwoI GCNNNNNNNGC 3 cut(s) 210, 219, 720
NdeI CATATG 2 cut(s) 1326, 1336
NdeII GATC 4 cut(s) 390, 668, 1318, 1381
NlaIII CATG 6 cut(s) 245, 314, 463, 970, 1000, 1123
NlaIV GGNNCC 4 cut(s) 300, 334, 861, 1164
NmuCI GTSAC 1 cut(s) 488
PaeR7I CTCGAG 1 cut(s) 738
PaqCI CACCTGC 1 cut(s) 605
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 2 cut(s) 990, 1387
PkrI GCNGC 3 cut(s) 553, 613, 769
Ple19I CGATCG 1 cut(s) 1384
PleI GAGTC 2 cut(s) 730, 994
PpsI GAGTC 2 cut(s) 730, 994
PpuMI RGGWCCY 1 cut(s) 1163
Psp124BI GAGCTC 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 1163
Psp6I CCWGG 2 cut(s) 172, 651
PspGI CCWGG 2 cut(s) 172, 651
PspN4I GGNNCC 4 cut(s) 300, 334, 861, 1164
PspPI GGNCC 2 cut(s) 1163, 1223
PspPPI RGGWCCY 1 cut(s) 1163
PstI CTGCAG 2 cut(s) 206, 616
PvuI CGATCG 1 cut(s) 1384
RsaI GTAC 2 cut(s) 350, 483
RsaNI GTAC 2 cut(s) 349, 482
RseI CAYNNNNRTG 2 cut(s) 840, 971
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 4 cut(s) 588, 686, 761, 1131
SatI GCNGC 3 cut(s) 552, 612, 768
Sau3AI GATC 4 cut(s) 390, 668, 1318, 1381
Sau96I GGNCC 2 cut(s) 1163, 1223
SchI GAGTC 2 cut(s) 730, 994
ScrFI CCNGG 2 cut(s) 174, 653
SduI GDGCHC 2 cut(s) 409, 681
SfaNI GCATC 1 cut(s) 1038
SfcI CTRYAG 3 cut(s) 202, 303, 612
Sfr274I CTCGAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 1163
SlaI CTCGAG 1 cut(s) 738
SmiI ATTTAAAT 1 cut(s) 687
SmiMI CAYNNNNRTG 2 cut(s) 840, 971
SmlI CTYRAG 1 cut(s) 738
SmoI CTYRAG 1 cut(s) 738
Sse9I AATT 6 cut(s) 12, 474, 688, 718, 900, 1238
SsiI CCGC 2 cut(s) 213, 947
SspI AATATT 1 cut(s) 983
SspMI CTAG 2 cut(s) 602, 1092
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 2 cut(s) 172, 651
SwaI ATTTAAAT 1 cut(s) 687
TaaI ACNGT 4 cut(s) 790, 1014, 1024, 1129
TaiI ACGT 1 cut(s) 1039
TaqI TCGA 4 cut(s) 739, 927, 1257, 1380
TasI AATT 6 cut(s) 12, 474, 688, 718, 900, 1238
TatI WGTACW 2 cut(s) 348, 481
TfiI GAWTC 2 cut(s) 990, 1387
Tru1I TTAA 4 cut(s) 588, 686, 761, 1131
Tru9I TTAA 4 cut(s) 588, 686, 761, 1131
TscAI CASTG 4 cut(s) 132, 206, 416, 1102
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 3 cut(s) 551, 611, 767
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 6 cut(s) 54, 299, 357, 785, 1047, 1323
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 4 cut(s) 132, 206, 416, 1102
VpaK11BI GGWCC 1 cut(s) 1163
XagI CCTNNNNNAGG 2 cut(s) 399, 605
XapI RAATTY 2 cut(s) 688, 1238
XhoI CTCGAG 1 cut(s) 738
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 2 cut(s) 602, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.