MD13G1240100.v1.1

Belongs to the glycosyl hydrolase 47 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001430
Physical Location & Seq
Forward (+)
24712821 .. 24720933
8113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1240100.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGCGAAAGAAATCATCATCTTCTTCTTCCTCATCACTGCCGTCGCAATCGTTGTGGCGGAGATGGTGGAGATATCTTCACCCGCGGTATCATCTGAAGAGGTCGCAGCTGTTGCCACTCATCGTCCTCTCCTTCGTCGCCCTCACATGGCTGCTCTCTGCCCTCCAATCCCTCACCGGAGACCACCAGGTAGCGGTGTCGAGGTTGAATAAGGAACTCAAAACTTTAAAGAATCAGCTAGAGAGTGTGAAGAAGCATTTAAGAGGTTGGAGCATTGTTGAGGAAGACGATGATCCTCTCAATTTCGAACGGAGAAAGGCGGTTAAAGATGCTATGATTCATGCCTGGACTTATTACGAGATGTATGCATGGGGTCATGATGAACTCAAGCCACAGACAAGAAATTCCGTTGATAGTTTTGGCGGTCTTGGTGCAACTCTAGTGGATTCTCTTGATACATTGTATATAATGGGTCTTGACCAGCAATTCCAAAGAGCTAGAGAGTGGGTTGCAAACTCATTGAATTTCAACAAGAATTATGAGGCTAGTGTTTTTGAGACAACCATAAGAGTTATAGGCGGACTTCTTAGCGCGTACGATCTCTCTCATGATGACATTTTCCTTGAAAAGGCAAGAGATATTGCAGATAGGTTGCTTCCTGCATGGAATACACCTTCAGGAATCCCCTATAACATAATAAACTTGATGTATGGGGATGCCAAGAACCCTCAGTGGACAGGGGGAAAAAGTATTCTGGCAGATGCTGGTTCAGAGCAGCTTGAATTTATTGCTCTATCTCAAAGGACGAAAGATCCCAAGTACCAGCAGAAGGTGGAATATGTCATCAAAGAGCTTCATAAAATATTCCCCGCTGATGGATTGCTTCCCATATATATCAATCCTCTCACTGGAGAAACTTCATACTCAAAAATTACATTTGGTGCCATGGGTGATAGTTTTTATGAATATCTACTTAAGGCTTGGATACAAGGGAACAAAACTGAAGCAGTAAAGAACTACAGAGAAATGTGGGAGACGTCAATGAAAGGTCTGAAAAGCTTGATTCGGAGGACGACGCCATCCTCATTTGCATATATATGTGAGAAGACTGGAAGCTCACTGTATGATAAGATGGATGAGTTAGCTTGCTTTGCTCCTGGCATGTTAGCTTTAGGATCTTCGGGCTATGTCCCTGATGAAGCTGAAAAGTTTTTATCCCTTGCTGAAGAGCTTGCGTGGACATGTTATAACTTCTACCAGAGAACTCCTACAAAATTGGCTGGAGAGAACTATTTCTTCCCAGCTGGACAGGACATGACTGTTGGAACATTGTGGAGCATTTTGAGGCCAGAGACAATTGAGTCACTGTTTTACCTATGGCGTTTCACTGGGAACAAAACTTACCAAGAATGGGGTTGGAATATTTTCCAAGCATTTGAAAACAACTCTAGAGTAGATTCAGGATACAGTGGGCTGAAAGATGTGAGTACAGGCGAGAAAGACGATATGATGCAGAGCTTCTTCCTTGCAGAAACGCTGAAGTATCTCTATCTCCTATTCTCGCCTCCATCATTCATCTCTTTGGATGAATGGGTATTCAACACAGAAGCACACCCTCTAAGGATCACGGATCGACATGCTAGTGGAGAGAGTGATAGTACAACTGAACAAGACAGATTACCTGAAACATTCCAAGGCAGGAAAGGACGGTCAGGGTCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 ko04141 map00510 map00513 map01100 map04141
Pfam Domains
Protein Families
Glyco_hydro_47

Protein Analysis

575

Amino Acids

65.79

Weight (kDa)

6.02

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_47 PF01532 111 - 542 2.4e-162 Glycosyl hydrolase family 47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1248
AasI GACNNNNNNGTC 1 cut(s) 1360
AatII GACGTC 1 cut(s) 1040
AccB1I GGYRCC 1 cut(s) 941
AccI GTMKAC 1 cut(s) 1718
AccII CGCG 2 cut(s) 85, 593
AciI CCGC 8 cut(s) 58, 83, 85, 194, 320, 423, 579, 870
AclWI GGATC 5 cut(s) 287, 806, 1183, 1631, 1638
AcsI RAATTY 3 cut(s) 403, 523, 782
AcuI CTGAAG 5 cut(s) 116, 660, 1023, 1245, 1559
AcyI GRCGYC 2 cut(s) 1037, 1076
AfaI GTAC 4 cut(s) 596, 821, 1489, 1660
AfiI CCNNNNNNNGG 7 cut(s) 147, 193, 628, 829, 875, 908, 1411
AflII CTTAAG 1 cut(s) 974
AflIII ACRYGT 1 cut(s) 1241
AgsI TTSAA 7 cut(s) 208, 523, 529, 626, 782, 1439, 1600
AjnI CCWGG 3 cut(s) 186, 344, 1156
Alw26I GTCTC 4 cut(s) 174, 551, 1028, 1346
AlwI GGATC 5 cut(s) 287, 806, 1183, 1631, 1638
AlwNI CAGNNNCTG 1 cut(s) 764
AoxI GGCC 1 cut(s) 1346
ApeKI GCWGC 3 cut(s) 106, 151, 775
ApoI RAATTY 3 cut(s) 403, 523, 782
Asp700I GAANNNNTTC 2 cut(s) 1292, 1424
AspLEI GCGC 1 cut(s) 593
AsuHPI GGTGA 3 cut(s) 71, 166, 962
AsuII TTCGAA 1 cut(s) 306
BanI GGYRCC 1 cut(s) 941
BbsI GAAGAC 2 cut(s) 291, 1112
BbvI GCAGC 3 cut(s) 118, 138, 787
BccI CCATC 5 cut(s) 57, 869, 1087, 1126, 1576
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 3 cut(s) 188, 346, 1158
BciVI GTATCC 2 cut(s) 978, 1457
BcoDI GTCTC 4 cut(s) 174, 551, 1028, 1346
BfaI CTAG 6 cut(s) 239, 440, 498, 546, 1449, 1641
BfmI CTRYAG 1 cut(s) 1018
BfrI CTTAAG 1 cut(s) 974
BfuI GTATCC 2 cut(s) 978, 1457
BisI GCNGC 3 cut(s) 107, 152, 776
BlsI GCNGC 3 cut(s) 108, 153, 777
Bme1390I CCNGG 3 cut(s) 188, 346, 1158
BmiI GGNNCC 1 cut(s) 943
BmrFI CCNGG 3 cut(s) 188, 346, 1158
BmrI ACTGGG 1 cut(s) 1398
BmsI GCATC 4 cut(s) 319, 706, 751, 1500
BmuI ACTGGG 1 cut(s) 1398
BpiI GAAGAC 2 cut(s) 291, 1112
BpmI CTGGAG 2 cut(s) 930, 1302
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 371
BsaHI GRCGYC 2 cut(s) 1037, 1076
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 83, 945, 1693
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 7 cut(s) 147, 193, 628, 829, 875, 908, 1411
Bse1I ACTGG 3 cut(s) 913, 1114, 1393
BseBI CCWGG 3 cut(s) 188, 346, 1158
BseDI CCNNGG 3 cut(s) 83, 945, 1693
BseGI GGATG 4 cut(s) 721, 1079, 1141, 1591
BseLI CCNNNNNNNGG 7 cut(s) 147, 193, 628, 829, 875, 908, 1411
BseMII CTCAG 1 cut(s) 743
BseNI ACTGG 3 cut(s) 913, 1114, 1393
BseXI GCAGC 3 cut(s) 118, 138, 787
BseYI CCCAGC 1 cut(s) 1300
Bsh1236I CGCG 2 cut(s) 85, 593
BshFI GGCC 1 cut(s) 1348
BshNI GGYRCC 1 cut(s) 941
BsiSI CCGG 1 cut(s) 177
BsiWI CGTACG 1 cut(s) 594
BslFI GGGAC 1 cut(s) 1175
BslI CCNNNNNNNGG 7 cut(s) 147, 193, 628, 829, 875, 908, 1411
BsmAI GTCTC 4 cut(s) 174, 551, 1028, 1346
BsmBI CGTCTC 1 cut(s) 1028
BsmFI GGGAC 1 cut(s) 1175
BsnI GGCC 1 cut(s) 1348
Bso31I GGTCTC 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 306
Bsp143I GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 8 cut(s) 58, 83, 85, 194, 320, 423, 579, 870
BspANI GGCC 1 cut(s) 1348
BspCNI CTCAG 1 cut(s) 742
BspFNI CGCG 2 cut(s) 85, 593
BspHI TCATGA 2 cut(s) 376, 607
BspLI GGNNCC 1 cut(s) 943
BspPI GGATC 5 cut(s) 287, 806, 1183, 1631, 1638
BspQI GCTCTTC 1 cut(s) 1221
BspT104I TTCGAA 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 941
BspTI CTTAAG 1 cut(s) 974
BspTNI GGTCTC 1 cut(s) 174
BsrI ACTGG 3 cut(s) 913, 1114, 1393
BssECI CCNNGG 3 cut(s) 83, 945, 1693
BssMI GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
BssNI GRCGYC 2 cut(s) 1037, 1076
BssT1I CCWWGG 2 cut(s) 945, 1693
Bst2UI CCWGG 3 cut(s) 188, 346, 1158
Bst4CI ACNGT 5 cut(s) 1122, 1321, 1368, 1469, 1710
Bst6I CTCTTC 2 cut(s) 92, 1221
BstACI GRCGYC 2 cut(s) 1037, 1076
BstAFI CTTAAG 1 cut(s) 974
BstAPI GCANNNNNTGC 1 cut(s) 112
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 1147, 1233
BstDEI CTNAG 4 cut(s) 587, 729, 1619, 1722
BstDSI CCRYGG 2 cut(s) 83, 945
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 4 cut(s) 721, 1079, 1141, 1591
BstFNI CGCG 2 cut(s) 85, 593
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 6 cut(s) 295, 601, 814, 1178, 1626, 1633
BstMAI GTCTC 4 cut(s) 174, 551, 1028, 1346
BstMBI GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
BstMWI GCNNNNNNNGC 2 cut(s) 112, 1151
BstNI CCWGG 3 cut(s) 188, 346, 1158
BstNSI RCATGY 3 cut(s) 1165, 1245, 1640
BstSCI CCNGG 3 cut(s) 186, 344, 1156
BstSFI CTRYAG 1 cut(s) 1018
BstUI CGCG 2 cut(s) 85, 593
BstV1I GCAGC 3 cut(s) 118, 138, 787
BstV2I GAAGAC 2 cut(s) 291, 1112
BstX2I RGATCY 2 cut(s) 811, 1175
BstYI RGATCY 2 cut(s) 811, 1175
BsuI GTATCC 2 cut(s) 978, 1457
BsuRI GGCC 1 cut(s) 1348
BtgI CCRYGG 2 cut(s) 83, 945
BtsCI GGATG 4 cut(s) 721, 1079, 1141, 1591
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 7 cut(s) 35, 737, 906, 1118, 1364, 1386, 1474
Cac8I GCNNGC 2 cut(s) 1147, 1233
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 2 cut(s) 376, 607
CfoI GCGC 1 cut(s) 593
Cfr42I CCGCGG 1 cut(s) 86
CseI GACGC 1 cut(s) 1084
CsiI ACCWGGT 1 cut(s) 186
Csp6I GTAC 4 cut(s) 595, 820, 1488, 1659
CspCI CAANNNNNGTGG 4 cut(s) 35, 70, 423, 458
CviQI GTAC 4 cut(s) 595, 820, 1488, 1659
DdeI CTNAG 4 cut(s) 587, 729, 1619, 1722
DpnI GATC 6 cut(s) 294, 600, 813, 1177, 1625, 1632
DpnII GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
DraI TTTAAA 1 cut(s) 228
DrdI GACNNNNNNGTC 1 cut(s) 1360
DseDI GACNNNNNNGTC 1 cut(s) 1360
Eam1104I CTCTTC 2 cut(s) 92, 1221
EarI CTCTTC 2 cut(s) 92, 1221
EciI GGCGGA 2 cut(s) 73, 594
Eco130I CCWWGG 2 cut(s) 945, 1693
Eco31I GGTCTC 1 cut(s) 174
Eco32I GATATC 1 cut(s) 74
Eco57I CTGAAG 5 cut(s) 116, 660, 1023, 1245, 1559
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoRII CCWGG 3 cut(s) 186, 344, 1156
EcoRV GATATC 1 cut(s) 74
EcoT14I CCWWGG 2 cut(s) 945, 1693
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 945, 1693
Esp3I CGTCTC 1 cut(s) 1028
FaqI GGGAC 1 cut(s) 1175
FauI CCCGC 2 cut(s) 90, 877
FblI GTMKAC 1 cut(s) 1718
Fnu4HI GCNGC 3 cut(s) 107, 152, 776
FokI GGATG 4 cut(s) 728, 1066, 1148, 1598
Fsp4HI GCNGC 3 cut(s) 107, 152, 776
FspBI CTAG 6 cut(s) 239, 440, 498, 546, 1449, 1641
GlaI GCGC 1 cut(s) 592
GluI GCNGC 3 cut(s) 107, 152, 776
GsaI CCCAGC 1 cut(s) 1304
GsuI CTGGAG 2 cut(s) 930, 1302
HaeIII GGCC 1 cut(s) 1348
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 1084
HhaI GCGC 1 cut(s) 593
Hin1I GRCGYC 2 cut(s) 1037, 1076
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HindIII AAGCTT 1 cut(s) 1057
HinfI GANTC 7 cut(s) 232, 337, 446, 681, 1063, 1361, 1457
HpaII CCGG 1 cut(s) 177
HphI GGTGA 3 cut(s) 71, 166, 962
Hpy166II GTNNAC 3 cut(s) 735, 1239, 1719
Hpy188I TCNGA 4 cut(s) 96, 772, 1053, 1068
Hpy188III TCNNGA 7 cut(s) 377, 452, 476, 608, 678, 1449, 1461
Hpy8I GTNNAC 3 cut(s) 735, 1239, 1719
Hpy99I CGWCG 3 cut(s) 46, 140, 1078
HpyAV CCTTC 3 cut(s) 142, 684, 823
HpyCH4III ACNGT 5 cut(s) 1122, 1321, 1368, 1469, 1710
HpyCH4IV ACGT 1 cut(s) 1037
HpyCH4V TGCA 8 cut(s) 368, 434, 512, 644, 662, 1091, 1513, 1529
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 1151
HpyF3I CTNAG 4 cut(s) 587, 729, 1619, 1722
HpySE526I ACGT 1 cut(s) 1037
Hsp92I GRCGYC 2 cut(s) 1037, 1076
HspAI GCGC 1 cut(s) 591
KspI CCGCGG 1 cut(s) 86
Kzo9I GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
LguI GCTCTTC 1 cut(s) 1221
LmnI GCTCC 3 cut(s) 270, 1159, 1335
Lsp1109I GCAGC 3 cut(s) 118, 138, 787
LweI GCATC 4 cut(s) 319, 706, 751, 1500
MabI ACCWGGT 1 cut(s) 186
MaeI CTAG 6 cut(s) 239, 440, 498, 546, 1449, 1641
MaeII ACGT 1 cut(s) 1037
MaeIII GTNAC 1 cut(s) 1362
MalI GATC 6 cut(s) 294, 600, 813, 1177, 1625, 1632
MboI GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
MfeI CAATTG 1 cut(s) 1356
MflI RGATCY 2 cut(s) 811, 1175
MluCI AATT 9 cut(s) 301, 403, 485, 523, 535, 782, 930, 1274, 1356
MlyI GAGTC 1 cut(s) 1370
MmeI TCCRAC 3 cut(s) 248, 1303, 1397
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 2 cut(s) 1292, 1424
MseI TTAA 4 cut(s) 227, 260, 324, 975
MslI CAYNNNNRTG 2 cut(s) 1096, 1641
MspA1I CMGCKG 4 cut(s) 85, 109, 872, 1304
MspCI CTTAAG 1 cut(s) 974
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 3 cut(s) 188, 346, 1158
MunI CAATTG 1 cut(s) 1356
MvaI CCWGG 3 cut(s) 188, 346, 1158
MvnI CGCG 2 cut(s) 85, 593
MwoI GCNNNNNNNGC 2 cut(s) 112, 1151
NcoI CCATGG 1 cut(s) 945
NdeII GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
NlaIV GGNNCC 1 cut(s) 943
NmuCI GTSAC 1 cut(s) 1362
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 3 cut(s) 1165, 1245, 1640
NspV TTCGAA 1 cut(s) 306
PagI TCATGA 2 cut(s) 376, 607
PciI ACATGT 1 cut(s) 1241
PciSI GCTCTTC 1 cut(s) 1221
PdmI GAANNNNTTC 2 cut(s) 1292, 1424
PfeI GAWTC 6 cut(s) 232, 337, 446, 681, 1063, 1457
Pfl23II CGTACG 1 cut(s) 594
PkrI GCNGC 3 cut(s) 108, 153, 777
PleI GAGTC 1 cut(s) 1369
PpsI GAGTC 1 cut(s) 1369
PscI ACATGT 1 cut(s) 1241
PsiI TTATAA 1 cut(s) 1248
Psp6I CCWGG 3 cut(s) 186, 344, 1156
PspFI CCCAGC 1 cut(s) 1300
PspGI CCWGG 3 cut(s) 186, 344, 1156
PspLI CGTACG 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 943
PsrI GAACNNNNNNTAC 2 cut(s) 1385, 1417
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 2 cut(s) 811, 1175
PvuII CAGCTG 2 cut(s) 109, 1304
RsaI GTAC 4 cut(s) 596, 821, 1489, 1660
RsaNI GTAC 4 cut(s) 595, 820, 1488, 1659
RseI CAYNNNNRTG 2 cut(s) 1096, 1641
SacII CCGCGG 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 1221
SaqAI TTAA 4 cut(s) 227, 260, 324, 975
SatI GCNGC 3 cut(s) 107, 152, 776
Sau3AI GATC 6 cut(s) 292, 598, 811, 1175, 1623, 1630
SchI GAGTC 1 cut(s) 1370
ScrFI CCNGG 3 cut(s) 188, 346, 1158
SexAI ACCWGGT 1 cut(s) 186
SfaNI GCATC 4 cut(s) 319, 706, 751, 1500
SfcI CTRYAG 1 cut(s) 1018
Sfr303I CCGCGG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 1096, 1641
SmlI CTYRAG 2 cut(s) 386, 974
SmoI CTYRAG 2 cut(s) 386, 974
Sse9I AATT 9 cut(s) 301, 403, 485, 523, 535, 782, 930, 1274, 1356
SsiI CCGC 8 cut(s) 58, 83, 85, 194, 320, 423, 579, 870
SspI AATATT 2 cut(s) 864, 1423
SspMI CTAG 6 cut(s) 239, 440, 498, 546, 1449, 1641
StyD4I CCNGG 3 cut(s) 186, 344, 1156
StyI CCWWGG 2 cut(s) 945, 1693
TaaI ACNGT 5 cut(s) 1122, 1321, 1368, 1469, 1710
TaiI ACGT 1 cut(s) 1040
TaqI TCGA 3 cut(s) 200, 306, 1633
TasI AATT 9 cut(s) 301, 403, 485, 523, 535, 782, 930, 1274, 1356
TatI WGTACW 2 cut(s) 1487, 1658
TfiI GAWTC 6 cut(s) 232, 337, 446, 681, 1063, 1457
Tru1I TTAA 4 cut(s) 227, 260, 324, 975
Tru9I TTAA 4 cut(s) 227, 260, 324, 975
TscAI CASTG 7 cut(s) 42, 737, 913, 1125, 1371, 1393, 1474
TseFI GTSAC 1 cut(s) 1362
TseI GCWGC 3 cut(s) 106, 151, 775
Tsp45I GTSAC 1 cut(s) 1362
TspDTI ATGAA 9 cut(s) 329, 396, 845, 909, 978, 1058, 1212, 1564, 1602
TspGWI ACGGA 3 cut(s) 325, 397, 1643
TspRI CASTG 7 cut(s) 42, 737, 913, 1125, 1371, 1393, 1474
Vha464I CTTAAG 1 cut(s) 974
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 3 cut(s) 403, 523, 782
XbaI TCTAGA 1 cut(s) 1448
XceI RCATGY 3 cut(s) 1165, 1245, 1640
XmiI GTMKAC 1 cut(s) 1718
XmnI GAANNNNTTC 2 cut(s) 1292, 1424
XspI CTAG 6 cut(s) 239, 440, 498, 546, 1449, 1641
ZraI GACGTC 1 cut(s) 1038
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.