Rh4DG085000

Belongs to the glycosyl hydrolase 47 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001430
Physical Location & Seq
Reverse (-)
12938306 .. 12951123
12818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG085000.1

Sequence Viewer

Length: 1287 bp
ATGGGAAAGAAATCTGCATCGTCTTTATCCTCACCGTCGTCGCCGCCTTCGTGGTGGTGGTGGAGATATCGATCGGCACTCGTCATAATCTCATTCGTGGCTCTCACATGGCTGCTCTTCGACCACCAATCCCTCACCTTGGAGGACCAGGTAGTGGTGTCCAGGTTGAATAAGGAGCTGAAAATGTTGAAGGATCAGTTAGAAAATGTGAAGAAGCATATAAGAGGCTGGAGCATTGTTGAAGAGGATATGGATGATCCTCTCAATGTAGAACGAAGAAATGCGGTTAAGGATGCTATGGTTCATGCCTGGACTTGTTACGAGAAGTATGCGTGGGGTCGTGATGAACTCCAGCCACAGACGAAAGACGCTGCTGACACTTTCGGTGGTCTAGGAGCAACTCTAGTGGATTCTCTTGATACATTGTATATAATGGGTCTTGACCAGCAATTCCAAAGAGCTAGAGAGTGGGTTGCAAGCTCGTTGAATTTCAACAAGAATTACGAGGCTAGTGTTTTTGAGACAACCATAAGAGTTATAGGCGGACTTCTTAGCGCATATGATCTGTCAGATGACAAACTCTTCCTTGACAAGGCTAGAGATATTGCAGATAGGTTGCTGCCTGCATGGAATACACCTTCTGGAATCCCATACAACATGATCAACTTGATGTATGGTAATGCCCATAACCCTAGGTGGGCAGGGGGAAAAAGTATTCTTGCAGATGCTGCTTCAGAACAGCTTGAATTTATTGCTTTATCTCAACGGACGGAAGACCCCAAGTACCAGCAGAAGGTGGAATATGTCATCAAAGAGTTTCATAAAATCTTCCCTGCTGATGGATTGCTTCCCATATATCTTGATCCTCACACTGGAACAATGTCATACTCAAAAGTTACATTCGGTGCCTTGGGTGATAGTTTTTATGAATATCTACTCAAGGCTTGGATACAAGGAAACAAAACAGAAGCTGTAAAACATTACAGAGAAATGTGGGAGACATCAATGAAAGGTTTGAAAAGCTTGATTCGGAGGACAACACCATCCTCGTTTGCATATATATGTGAAAAGCTTGGAAGCTCACTAACTGACAAGATGGATGAATTAGCTTGCTTTGCTCCTGGCATGCTGGCTTTAGGATCTTCTGGCTATGACCCTGGCGAAGCTGAAAAGATTTTATCCCTTGCTGAAGAGCTTGCATGGACATGTTACAATTTCTATCAGACAACGCCTACAAAATTGGCTGGGGAGAACTATAACTTTCCCGCTGGACAGGTCTTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 ko04141 map00510 map00513 map01100 map04141
Pfam Domains
Protein Families
Glyco_hydro_47

Protein Analysis

428

Amino Acids

48.57

Weight (kDa)

5.44

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_47 PF01532 99 - 422 2.3e-107 Glycosyl hydrolase family 47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 154
AciI CCGC 4 cut(s) 44, 284, 543, 1266
AclWI GGATC 4 cut(s) 201, 251, 857, 1147
AcsI RAATTY 2 cut(s) 487, 746
AcuI CTGAAG 2 cut(s) 717, 1209
AfaI GTAC 1 cut(s) 785
AfiI CCNNNNNNNGG 7 cut(s) 139, 154, 592, 697, 793, 839, 872
AflIII ACRYGT 1 cut(s) 1205
AgsI TTSAA 7 cut(s) 169, 190, 242, 487, 493, 746, 1018
AjnI CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
Alw26I GTCTC 2 cut(s) 515, 992
AlwI GGATC 4 cut(s) 201, 251, 857, 1147
AlwNI CAGNNNCTG 2 cut(s) 728, 971
ApeKI GCWGC 4 cut(s) 112, 371, 619, 728
ApoI RAATTY 2 cut(s) 487, 746
AspA2I CCTAGG 1 cut(s) 692
AspLEI GCGC 1 cut(s) 557
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 3 cut(s) 24, 127, 926
AvaII GGWCC 1 cut(s) 145
AvrII CCTAGG 1 cut(s) 692
BanI GGYRCC 1 cut(s) 905
BbsI GAAGAC 1 cut(s) 780
BbvI GCAGC 4 cut(s) 99, 358, 606, 715
BccI CCATC 3 cut(s) 833, 1051, 1090
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
BciVI GTATCC 1 cut(s) 942
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 515, 992
BfaI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
BfuI GTATCC 1 cut(s) 942
BisI GCNGC 5 cut(s) 44, 113, 372, 620, 729
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 5 cut(s) 45, 114, 373, 621, 730
Bme1390I CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
BmsI GCATC 3 cut(s) 26, 283, 715
BpiI GAAGAC 1 cut(s) 780
BpmI CTGGAG 2 cut(s) 250, 335
BpuEI CTTGAG 1 cut(s) 923
Bsa29I ATCGAT 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 70
BsaJI CCNNGG 4 cut(s) 138, 692, 909, 1156
Bsc4I CCNNNNNNNGG 7 cut(s) 139, 154, 592, 697, 793, 839, 872
Bse1I ACTGG 1 cut(s) 877
Bse8I GATNNNNATC 1 cut(s) 70
BseBI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
BseCI ATCGAT 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 138, 692, 909, 1156
BseGI GGATG 4 cut(s) 259, 298, 1043, 1105
BseJI GATNNNNATC 1 cut(s) 70
BseLI CCNNNNNNNGG 7 cut(s) 139, 154, 592, 697, 793, 839, 872
BseNI ACTGG 1 cut(s) 877
BseXI GCAGC 4 cut(s) 99, 358, 606, 715
BseYI CCCAGC 1 cut(s) 1244
Bsh1285I CGRYCG 1 cut(s) 74
BshNI GGYRCC 1 cut(s) 905
BshVI ATCGAT 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 74
BslI CCNNNNNNNGG 7 cut(s) 139, 154, 592, 697, 793, 839, 872
BsmAI GTCTC 2 cut(s) 515, 992
Bsp143I GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
BspACI CCGC 4 cut(s) 44, 284, 543, 1266
BspDI ATCGAT 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 907
BspPI GGATC 4 cut(s) 201, 251, 857, 1147
BspQI GCTCTTC 2 cut(s) 122, 1185
BspT107I GGYRCC 1 cut(s) 905
BsrI ACTGG 1 cut(s) 877
BssECI CCNNGG 4 cut(s) 138, 692, 909, 1156
BssMI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
BssT1I CCWWGG 3 cut(s) 138, 692, 909
Bst2UI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
Bst4CI ACNGT 1 cut(s) 36
Bst6I CTCTTC 4 cut(s) 122, 237, 587, 1185
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 6 cut(s) 478, 624, 1111, 1127, 1131, 1197
BstDEI CTNAG 1 cut(s) 551
BstENI CCTNNNNNAGG 1 cut(s) 590
BstF5I GGATG 4 cut(s) 259, 298, 1043, 1105
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 7 cut(s) 74, 196, 259, 565, 663, 865, 1142
BstMAI GTCTC 2 cut(s) 515, 992
BstMBI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 2 cut(s) 728, 1115
BstNI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
BstNSI RCATGY 2 cut(s) 1129, 1209
BstSCI CCNGG 5 cut(s) 147, 161, 308, 1120, 1156
BstV1I GCAGC 4 cut(s) 99, 358, 606, 715
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 1 cut(s) 1139
BstYI RGATCY 1 cut(s) 1139
Bsu15I ATCGAT 1 cut(s) 70
BsuI GTATCC 1 cut(s) 942
BsuTUI ATCGAT 1 cut(s) 70
BtsCI GGATG 4 cut(s) 259, 298, 1043, 1105
BtsIMutI CAGTG 1 cut(s) 870
Cac8I GCNNGC 6 cut(s) 478, 624, 1111, 1127, 1131, 1197
CaiI CAGNNNCTG 2 cut(s) 728, 971
CfoI GCGC 1 cut(s) 557
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 70
CseI GACGC 1 cut(s) 377
CsiI ACCWGGT 1 cut(s) 147
Csp6I GTAC 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviAII CATG 7 cut(s) 108, 305, 627, 658, 1126, 1200, 1206
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 1 cut(s) 551
DpnI GATC 7 cut(s) 73, 195, 258, 564, 662, 864, 1141
DpnII GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
Eam1104I CTCTTC 4 cut(s) 122, 237, 587, 1185
EarI CTCTTC 4 cut(s) 122, 237, 587, 1185
EciI GGCGGA 1 cut(s) 558
Eco130I CCWWGG 3 cut(s) 138, 692, 909
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 145
Eco57I CTGAAG 2 cut(s) 717, 1209
EcoNI CCTNNNNNAGG 1 cut(s) 590
EcoRII CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
EcoRV GATATC 1 cut(s) 68
EcoT14I CCWWGG 3 cut(s) 138, 692, 909
ErhI CCWWGG 3 cut(s) 138, 692, 909
FaeI CATG 7 cut(s) 111, 308, 630, 661, 1129, 1203, 1209
FatI CATG 7 cut(s) 107, 304, 626, 657, 1125, 1199, 1205
FauI CCCGC 1 cut(s) 1273
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 44, 113, 372, 620, 729
FokI GGATG 4 cut(s) 266, 305, 1030, 1112
Fsp4HI GCNGC 5 cut(s) 44, 113, 372, 620, 729
FspBI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
GlaI GCGC 1 cut(s) 556
GluI GCNGC 5 cut(s) 44, 113, 372, 620, 729
GsaI CCCAGC 1 cut(s) 1248
GsuI CTGGAG 2 cut(s) 250, 335
HgaI GACGC 1 cut(s) 377
HhaI GCGC 1 cut(s) 557
Hin1II CATG 7 cut(s) 111, 308, 630, 661, 1129, 1203, 1209
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HindIII AAGCTT 2 cut(s) 1021, 1070
HinfI GANTC 3 cut(s) 410, 645, 1027
HphI GGTGA 3 cut(s) 24, 127, 926
Hpy188I TCNGA 4 cut(s) 571, 736, 1032, 1224
Hpy188III TCNNGA 5 cut(s) 341, 416, 440, 642, 860
Hpy99I CGWCG 2 cut(s) 40, 43
HpyAV CCTTC 4 cut(s) 57, 184, 648, 787
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 7 cut(s) 17, 476, 608, 626, 722, 1055, 1199
HpyF10VI GCNNNNNNNGC 2 cut(s) 728, 1115
HpyF3I CTNAG 1 cut(s) 551
Hsp92II CATG 7 cut(s) 111, 308, 630, 661, 1129, 1203, 1209
HspAI GCGC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
LguI GCTCTTC 2 cut(s) 122, 1185
LmnI GCTCC 4 cut(s) 175, 231, 395, 1123
Lsp1109I GCAGC 4 cut(s) 99, 358, 606, 715
LweI GCATC 3 cut(s) 26, 283, 715
MabI ACCWGGT 1 cut(s) 147
MaeI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
MaeIII GTNAC 3 cut(s) 317, 895, 1208
MalI GATC 7 cut(s) 73, 195, 258, 564, 662, 864, 1141
MboI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
MboII GAAGA 9 cut(s) 109, 223, 254, 288, 574, 785, 820, 1134, 1202
MflI RGATCY 1 cut(s) 1139
MluCI AATT 7 cut(s) 449, 487, 499, 746, 1103, 1213, 1238
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 2 cut(s) 1060, 1204
MspA1I CMGCKG 1 cut(s) 1268
MspR9I CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
MvaI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
MwoI GCNNNNNNNGC 2 cut(s) 728, 1115
NdeI CATATG 1 cut(s) 559
NdeII GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
NlaIII CATG 7 cut(s) 111, 308, 630, 661, 1129, 1203, 1209
NlaIV GGNNCC 1 cut(s) 907
NspI RCATGY 2 cut(s) 1129, 1209
PaeI GCATGC 1 cut(s) 1129
PciI ACATGT 1 cut(s) 1205
PciSI GCTCTTC 2 cut(s) 122, 1185
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 3 cut(s) 410, 645, 1027
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 5 cut(s) 45, 114, 373, 621, 730
Ple19I CGATCG 1 cut(s) 74
PscI ACATGT 1 cut(s) 1205
Psp6I CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
PspFI CCCAGC 1 cut(s) 1244
PspGI CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
PspN4I GGNNCC 1 cut(s) 907
PspPI GGNCC 1 cut(s) 145
PstNI CAGNNNCTG 2 cut(s) 728, 971
PsuI RGATCY 1 cut(s) 1139
PvuI CGATCG 1 cut(s) 74
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
RseI CAYNNNNRTG 2 cut(s) 1060, 1204
SapI GCTCTTC 2 cut(s) 122, 1185
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 5 cut(s) 44, 113, 372, 620, 729
Sau3AI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
SexAI ACCWGGT 1 cut(s) 147
SfaNI GCATC 3 cut(s) 26, 283, 715
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 1060, 1204
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
SphI GCATGC 1 cut(s) 1129
Sse9I AATT 7 cut(s) 449, 487, 499, 746, 1103, 1213, 1238
SsiI CCGC 4 cut(s) 44, 284, 543, 1266
SspMI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
StyD4I CCNGG 5 cut(s) 147, 161, 308, 1120, 1156
StyI CCWWGG 3 cut(s) 138, 692, 909
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 2 cut(s) 70, 120
TasI AATT 7 cut(s) 449, 487, 499, 746, 1103, 1213, 1238
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 3 cut(s) 410, 645, 1027
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 877
TseI GCWGC 4 cut(s) 112, 371, 619, 728
TspDTI ATGAA 6 cut(s) 293, 360, 809, 942, 1022, 1116
TspGWI ACGGA 2 cut(s) 781, 785
TspRI CASTG 1 cut(s) 877
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 145
XagI CCTNNNNNAGG 1 cut(s) 590
XapI RAATTY 2 cut(s) 487, 746
XceI RCATGY 2 cut(s) 1129, 1209
XmaJI CCTAGG 1 cut(s) 692
XspI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.