MD13G1285500.v1.1

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009675
Physical Location & Seq
Forward (+)
44048059 .. 44056053
7995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1285500.v1.1.491

Sequence Viewer

Length: 1788 bp
ATGATCTCGATTGGGTCTCTGAAACCAAAAATGGAGACGACAGCAATTGCGATTGCGGGAGACTTGTTTTCTTGTTTTGAAACAAGAAGCAGCAGCTTATGTCATCCTCCTCCGACGAGCACCAGATGGAGGTGGCGGAGGCGGAGGCAGAAGCAGAAGCAGTCTTGCTCTTGTCAATTCCGCATTCCACAGGAATTCGCTTGGCCTTCTCCTGATGATGATATCCCCTTTTGGAAGAAGCCCTTCCCTTCTTGGGACGTCAACTTGGAACCTCCCTCCCAAGTAATTAAAGATTCCAAACTCATGCACCTAGTTCATGTCACCGCTGAAATGGCACCCATTGCCAAAGTTGGTGGTCTTGCTGATGTTGTCACTGGACTTGCTCGAGCTTCTTTATCGCGCGGCCACACTGTACATGTAATGCTTCCTTTCTACGAGTGTATCCAGAAGCACCAGATCAATGACCTCTCGTTGGTCGCAACATATAACTCTTTTCACCATGGAAACTGGATTCCCACTAATGCATACACTGGGATAGTTTCAGGAATCCCAGTTATACTTATTGACCCATCACATCACTTCTTTAAGGGCCATCATGTCTATGGGGGTTCCTACAATGAGCTCGAAGCATATCTCTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGGCGACTGGAACACAACCTGATATTATTCATGTCCATGAATGGCAGACAGGTGCTTTGCCGTTACTTTATTGGGATATGTATCATTATCTCTCACTTAAGAAACCAAGAGTAGTATTGACAATCCATAACATGGAGCACTATGGTGAATGTAGACAAGAGCAACTGAGCATGTGCGGTCTTGATGGATCTTTGTATGCTAACTTTGAAAAAGCAATTGATGATCGAACTGTTGGCCATAATCCTGAGAGGTTAAGCTTATTGAAAGGTGGAATCGTCTACAGCAATGCAGTAGTTACAGTTTCTCCAACATATCTCAAGGAAACACTCTGCTCTGGATGGCTTTCAAGTACTTTGATAAGAAACCGTGATAAGTACTTTGGTATAGTAAATGGAATAGACACTGCGATGTGGGACCCTTCTACTGATGTTTTCTTGCCTTCAAAGTTTCGTGCTCAAAACCCTGAGGGCAAGAAATTATGCAAATACTATGTCCAAAGGGGACTTGGTTTGGCCTCAGGCGACCATGTGCCAGATACTGCACTCAAGGTTCCCTTGGTTGTATGTATCACTCGTTTAGTTGCACAAAAGGGTCTGCATTTGATTACTCATGCAATTAAGCAAGTTGAGGAACTTGGTGGACAAATGGTTATACTGGGAAAAGCTCCTGATTCTCGGGTTGAAAGTGAATTTGAAGGTCTTGCAAAATTGCATAACCAAGGTCCCAGCATTCGGATATTATTGATGTATAGTGAGGAGCTATCCCACTTGCTGTATGCCGCTGCAGACTTTATTTTAGTTCCTTCCATGTACGAGCCATGTGGACTTGCCCAAATGATTGGAATGCGTTATGGGGCAGTCCCAGTAGTTAGAAAGACCGGCGGTCTTGCAGACACAGTCTTTGACATGGATGATGAACAAAATCATGACATGGCCAATGGATTTGTTTTTGAAGGAATCGACGAAGGATCCCTGAATGGGGCTCTAGGTCGTGCATTTGCTTGCTACAGAAACAGAGGAGATGAATGGAATGGCATTGTTAAGAAGGTTATGGAAATTGATAATGGCTGGAACAACACAGCTGGCAAGTACATTAAGATTTATGAATCAGTTAGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

596

Amino Acids

66.75

Weight (kDa)

6.92

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 104 - 331 8.1e-55 Starch synthase catalytic domain
Glyco_transf_4 PF13439 117 - 294 1.2e-09 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 405 - 547 4.2e-11 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 407 - 525 6.7e-09 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 334
AccB7I CCANNNNNTGG 1 cut(s) 799
AccI GTMKAC 2 cut(s) 820, 945
AccII CGCG 2 cut(s) 400, 402
AciI CCGC 9 cut(s) 56, 136, 142, 181, 324, 402, 843, 1446, 1548
AclWI GGATC 3 cut(s) 862, 1629, 1642
AcoI YGGCCR 3 cut(s) 403, 901, 1599
AcsI RAATTY 2 cut(s) 194, 1355
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 5 cut(s) 414, 1018, 1043, 1478, 1757
AfiI CCNNNNNNNGG 7 cut(s) 129, 253, 472, 799, 1398, 1644, 1645
AflII CTTAAG 1 cut(s) 764
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 9 cut(s) 80, 659, 875, 931, 1014, 1110, 1349, 1361, 1619
AhdI GACNNNNNGTC 1 cut(s) 1548
AleI CACNNNNGTG 1 cut(s) 810
AluBI AGCT 7 cut(s) 96, 389, 622, 924, 1331, 1426, 1748
AluI AGCT 7 cut(s) 96, 389, 622, 924, 1331, 1426, 1748
Alw21I GWGCWC 4 cut(s) 122, 624, 807, 1123
Alw26I GTCTC 3 cut(s) 21, 29, 54
AlwI GGATC 3 cut(s) 862, 1629, 1642
AlwNI CAGNNNCTG 1 cut(s) 1205
Ama87I CYCGRG 2 cut(s) 384, 1341
AoxI GGCC 6 cut(s) 203, 403, 589, 901, 1179, 1599
ApeKI GCWGC 3 cut(s) 90, 93, 1448
ApoI RAATTY 2 cut(s) 194, 1355
ArsI GACNNNNNNTTYG 6 cut(s) 706, 738, 1143, 1175, 1550, 1582
Asp700I GAANNNNTTC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 3 cut(s) 589, 1081, 1388
AsuHPI GGTGA 3 cut(s) 313, 488, 824
AvaI CYCGRG 2 cut(s) 384, 1341
AvaII GGWCC 2 cut(s) 1081, 1388
AxyI CCTNAGG 2 cut(s) 1131, 1183
BaeI ACNNNNGTAYC 2 cut(s) 424, 457
BalI TGGCCA 2 cut(s) 903, 1601
BamHI GGATCC 1 cut(s) 1634
BanI GGYRCC 1 cut(s) 334
BanII GRGCYC 2 cut(s) 624, 1651
Bbv12I GWGCWC 4 cut(s) 122, 624, 807, 1123
BbvI GCAGC 3 cut(s) 102, 105, 1435
BccI CCATC 5 cut(s) 120, 577, 600, 845, 999
BceAI ACGGC 1 cut(s) 712
BciVI GTATCC 1 cut(s) 452
BcoDI GTCTC 3 cut(s) 21, 29, 54
BfaI CTAG 2 cut(s) 311, 1652
BfmI CTRYAG 3 cut(s) 946, 1449, 1672
BfrI CTTAAG 1 cut(s) 764
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 5 cut(s) 91, 94, 403, 1446, 1449
BlsI GCNGC 5 cut(s) 92, 95, 404, 1447, 1450
BmcAI AGTACT 2 cut(s) 1018, 1043
Bme18I GGWCC 2 cut(s) 1081, 1388
BmeRI GACNNNNNGTC 1 cut(s) 1548
BmeT110I CYCGRG 2 cut(s) 384, 1341
BmgT120I GGNCC 3 cut(s) 589, 1081, 1388
BmiI GGNNCC 8 cut(s) 270, 336, 610, 1082, 1083, 1218, 1390, 1636
BmrI ACTGGG 4 cut(s) 540, 545, 1331, 1523
BmsI GCATC 1 cut(s) 654
BmuI ACTGGG 4 cut(s) 540, 545, 1331, 1523
BpuEI CTTGAG 2 cut(s) 968, 1196
BsaBI GATNNNNATC 1 cut(s) 747
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 499, 1221, 1384
BsaXI ACNNNNNCTCC 2 cut(s) 955, 985
Bsc4I CCNNNNNNNGG 7 cut(s) 129, 253, 472, 799, 1398, 1644, 1645
Bse118I RCCGGY 1 cut(s) 1544
Bse1I ACTGG 7 cut(s) 379, 512, 535, 551, 679, 1326, 1529
Bse21I CCTNAGG 2 cut(s) 1131, 1183
Bse3DI GCAATG 2 cut(s) 339, 958
Bse8I GATNNNNATC 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 499, 1221, 1384
BseGI GGATG 4 cut(s) 103, 669, 1010, 1582
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 7 cut(s) 129, 253, 472, 799, 1398, 1644, 1645
BseMI GCAATG 2 cut(s) 339, 958
BseMII CTCAG 4 cut(s) 824, 903, 1122, 1197
BseNI ACTGG 7 cut(s) 379, 512, 535, 551, 679, 1326, 1529
BseRI GAGGAG 3 cut(s) 99, 1436, 1698
BseXI GCAGC 3 cut(s) 102, 105, 1435
BseYI CCCAGC 1 cut(s) 1391
BsgI GTGCAG 1 cut(s) 1191
Bsh1236I CGCG 2 cut(s) 400, 402
BshFI GGCC 6 cut(s) 205, 405, 591, 903, 1181, 1601
BshNI GGYRCC 1 cut(s) 334
BsiHKAI GWGCWC 4 cut(s) 122, 624, 807, 1123
BsiHKCI CYCGRG 2 cut(s) 384, 1341
BsiSI CCGG 1 cut(s) 1545
BslFI GGGAC 5 cut(s) 269, 1094, 1182, 1374, 1511
BslI CCNNNNNNNGG 7 cut(s) 129, 253, 472, 799, 1398, 1644, 1645
BsmAI GTCTC 3 cut(s) 21, 29, 54
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 5 cut(s) 269, 1094, 1182, 1374, 1511
BsmI GAATGC 3 cut(s) 183, 1395, 1515
BsnI GGCC 6 cut(s) 205, 405, 591, 903, 1181, 1601
Bso31I GGTCTC 1 cut(s) 21
BsoBI CYCGRG 2 cut(s) 384, 1341
Bsp1286I GDGCHC 5 cut(s) 122, 624, 807, 1123, 1651
Bsp1407I TGTACA 1 cut(s) 412
Bsp143I GATC 5 cut(s) 3, 456, 854, 889, 1634
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 9 cut(s) 56, 136, 142, 181, 324, 402, 843, 1446, 1548
BspANI GGCC 6 cut(s) 205, 405, 591, 903, 1181, 1601
BspCNI CTCAG 4 cut(s) 825, 904, 1123, 1196
BspFNI CGCG 2 cut(s) 400, 402
BspHI TCATGA 1 cut(s) 1591
BspLI GGNNCC 8 cut(s) 270, 336, 610, 1082, 1083, 1218, 1390, 1636
BspMAI CTGCAG 1 cut(s) 1453
BspPI GGATC 3 cut(s) 862, 1629, 1642
BspT107I GGYRCC 1 cut(s) 334
BspTI CTTAAG 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 21
BsrDI GCAATG 2 cut(s) 339, 958
BsrFI RCCGGY 1 cut(s) 1544
BsrGI TGTACA 1 cut(s) 412
BsrI ACTGG 7 cut(s) 379, 512, 535, 551, 679, 1326, 1529
BssAI RCCGGY 1 cut(s) 1544
BssECI CCNNGG 3 cut(s) 499, 1221, 1384
BssMI GATC 5 cut(s) 3, 456, 854, 889, 1634
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 3 cut(s) 499, 1221, 1384
Bst4CI ACNGT 5 cut(s) 412, 898, 967, 1034, 1564
Bst6I CTCTTC 1 cut(s) 641
BstACI GRCGYC 1 cut(s) 258
BstAFI CTTAAG 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 341
BstAUI TGTACA 1 cut(s) 412
BstC8I GCNNGC 4 cut(s) 652, 669, 1669, 1750
BstDEI CTNAG 4 cut(s) 833, 912, 1131, 1183
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 4 cut(s) 103, 669, 1010, 1582
BstFNI CGCG 2 cut(s) 400, 402
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 5 cut(s) 6, 459, 857, 892, 1637
BstMAI GTCTC 3 cut(s) 21, 29, 54
BstMBI GATC 5 cut(s) 3, 456, 854, 889, 1634
BstMWI GCNNNNNNNGC 2 cut(s) 332, 341
BstNSI RCATGY 2 cut(s) 419, 841
BstSFI CTRYAG 3 cut(s) 946, 1449, 1672
BstUI CGCG 2 cut(s) 400, 402
BstV1I GCAGC 3 cut(s) 102, 105, 1435
BstX2I RGATCY 2 cut(s) 854, 1634
BstXI CCANNNNNNTGG 1 cut(s) 1505
BstYI RGATCY 2 cut(s) 854, 1634
Bsu36I CCTNAGG 2 cut(s) 1131, 1183
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 6 cut(s) 205, 405, 591, 903, 1181, 1601
BtgI CCRYGG 1 cut(s) 499
BtgZI GCGATG 1 cut(s) 1088
BtsCI GGATG 4 cut(s) 103, 669, 1010, 1582
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 4 cut(s) 372, 408, 528, 1068
Cac8I GCNNGC 4 cut(s) 652, 669, 1669, 1750
CaiI CAGNNNCTG 1 cut(s) 1205
CciI TCATGA 1 cut(s) 1591
CfoI GCGC 1 cut(s) 402
Cfr10I RCCGGY 1 cut(s) 1544
Cfr13I GGNCC 3 cut(s) 589, 1081, 1388
Csp6I GTAC 5 cut(s) 413, 1017, 1042, 1477, 1756
CspCI CAANNNNNGTGG 2 cut(s) 334, 369
CviQI GTAC 5 cut(s) 413, 1017, 1042, 1477, 1756
DdeI CTNAG 4 cut(s) 833, 912, 1131, 1183
DpnI GATC 5 cut(s) 5, 458, 856, 891, 1636
DpnII GATC 5 cut(s) 3, 456, 854, 889, 1634
DriI GACNNNNNGTC 1 cut(s) 1548
EaeI YGGCCR 3 cut(s) 403, 901, 1599
Eam1104I CTCTTC 1 cut(s) 641
Eam1105I GACNNNNNGTC 1 cut(s) 1548
EarI CTCTTC 1 cut(s) 641
EciI GGCGGA 2 cut(s) 151, 157
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 3 cut(s) 499, 1221, 1384
Eco24I GRGCYC 2 cut(s) 624, 1651
Eco31I GGTCTC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 1081, 1388
Eco53kI GAGCTC 1 cut(s) 622
Eco81I CCTNAGG 2 cut(s) 1131, 1183
Eco88I CYCGRG 2 cut(s) 384, 1341
EcoICRI GAGCTC 1 cut(s) 622
EcoO109I RGGNCCY 2 cut(s) 1081, 1388
EcoRI GAATTC 1 cut(s) 194
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 3 cut(s) 499, 1221, 1384
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 2 cut(s) 624, 1651
ErhI CCWWGG 3 cut(s) 499, 1221, 1384
Esp3I CGTCTC 1 cut(s) 29
FalI AAGNNNNNCTT 4 cut(s) 227, 259, 1205, 1237
FaqI GGGAC 5 cut(s) 269, 1094, 1182, 1374, 1511
FauI CCCGC 1 cut(s) 49
FblI GTMKAC 2 cut(s) 820, 945
Fnu4HI GCNGC 5 cut(s) 91, 94, 403, 1446, 1449
FokI GGATG 4 cut(s) 90, 676, 1017, 1589
FriOI GRGCYC 2 cut(s) 624, 1651
Fsp4HI GCNGC 5 cut(s) 91, 94, 403, 1446, 1449
FspBI CTAG 2 cut(s) 311, 1652
GlaI GCGC 1 cut(s) 401
GluI GCNGC 5 cut(s) 91, 94, 403, 1446, 1449
GsaI CCCAGC 1 cut(s) 1395
HaeIII GGCC 6 cut(s) 205, 405, 591, 903, 1181, 1601
HapII CCGG 1 cut(s) 1545
HhaI GCGC 1 cut(s) 402
Hin1I GRCGYC 1 cut(s) 258
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 922
HinfI GANTC 7 cut(s) 293, 511, 546, 939, 1337, 1623, 1772
HpaII CCGG 1 cut(s) 1545
HphI GGTGA 3 cut(s) 313, 488, 824
Hpy166II GTNNAC 5 cut(s) 262, 821, 946, 1307, 1490
Hpy188I TCNGA 3 cut(s) 21, 114, 1401
Hpy188III TCNNGA 9 cut(s) 7, 212, 445, 543, 848, 911, 1002, 1334, 1592
Hpy8I GTNNAC 5 cut(s) 262, 821, 946, 1307, 1490
Hpy99I CGWCG 2 cut(s) 118, 1631
HpyCH4III ACNGT 5 cut(s) 412, 898, 967, 1034, 1564
HpyCH4IV ACGT 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 341
HpyF3I CTNAG 4 cut(s) 833, 912, 1131, 1183
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 258
HspAI GCGC 1 cut(s) 400
KflI GGGWCCC 1 cut(s) 1081
Kzo9I GATC 5 cut(s) 3, 456, 854, 889, 1634
LmnI GCTCC 3 cut(s) 802, 1336, 1423
Lsp1109I GCAGC 3 cut(s) 102, 105, 1435
LweI GCATC 1 cut(s) 654
MaeI CTAG 2 cut(s) 311, 1652
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 4 cut(s) 319, 370, 729, 961
MalI GATC 5 cut(s) 5, 458, 856, 891, 1636
MboI GATC 5 cut(s) 3, 456, 854, 889, 1634
MboII GAAGA 2 cut(s) 247, 628
MfeI CAATTG 2 cut(s) 45, 882
MflI RGATCY 2 cut(s) 854, 1634
MhlI GDGCHC 5 cut(s) 122, 624, 807, 1123, 1651
MlsI TGGCCA 2 cut(s) 903, 1601
MluNI TGGCCA 2 cut(s) 903, 1601
MmeI TCCRAC 2 cut(s) 137, 998
Mox20I TGGCCA 2 cut(s) 903, 1601
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 242
MscI TGGCCA 2 cut(s) 903, 1601
MseI TTAA 7 cut(s) 288, 585, 765, 920, 1284, 1707, 1761
MslI CAYNNNNRTG 4 cut(s) 600, 702, 810, 1073
Msp20I TGGCCA 2 cut(s) 903, 1601
MspA1I CMGCKG 3 cut(s) 326, 1448, 1748
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 1 cut(s) 1545
MunI CAATTG 2 cut(s) 45, 882
Mva1269I GAATGC 3 cut(s) 183, 1395, 1515
MvnI CGCG 2 cut(s) 400, 402
MwoI GCNNNNNNNGC 2 cut(s) 332, 341
NcoI CCATGG 1 cut(s) 499
NdeII GATC 5 cut(s) 3, 456, 854, 889, 1634
NlaIV GGNNCC 8 cut(s) 270, 336, 610, 1082, 1083, 1218, 1390, 1636
NmuCI GTSAC 2 cut(s) 319, 370
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 2 cut(s) 419, 841
OliI CACNNNNGTG 1 cut(s) 810
PaeR7I CTCGAG 1 cut(s) 384
PagI TCATGA 1 cut(s) 1591
PciI ACATGT 1 cut(s) 415
PctI GAATGC 3 cut(s) 183, 1395, 1515
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 7 cut(s) 293, 511, 546, 939, 1337, 1623, 1772
PflFI GACNNNGTC 1 cut(s) 1562
PflMI CCANNNNNTGG 1 cut(s) 799
PkrI GCNGC 5 cut(s) 92, 95, 404, 1447, 1450
PpuMI RGGWCCY 2 cut(s) 1081, 1388
PscI ACATGT 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 624
Psp5II RGGWCCY 2 cut(s) 1081, 1388
PspFI CCCAGC 1 cut(s) 1391
PspN4I GGNNCC 8 cut(s) 270, 336, 610, 1082, 1083, 1218, 1390, 1636
PspPI GGNCC 3 cut(s) 589, 1081, 1388
PspPPI RGGWCCY 2 cut(s) 1081, 1388
PspXI VCTCGAGB 1 cut(s) 384
PstI CTGCAG 1 cut(s) 1453
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 2 cut(s) 854, 1634
PsyI GACNNNGTC 1 cut(s) 1562
PvuII CAGCTG 1 cut(s) 1748
RsaI GTAC 5 cut(s) 414, 1018, 1043, 1478, 1757
RsaNI GTAC 5 cut(s) 413, 1017, 1042, 1477, 1756
RseI CAYNNNNRTG 4 cut(s) 600, 702, 810, 1073
SacI GAGCTC 1 cut(s) 624
SaqAI TTAA 7 cut(s) 288, 585, 765, 920, 1284, 1707, 1761
SatI GCNGC 5 cut(s) 91, 94, 403, 1446, 1449
Sau3AI GATC 5 cut(s) 3, 456, 854, 889, 1634
Sau96I GGNCC 3 cut(s) 589, 1081, 1388
ScaI AGTACT 2 cut(s) 1018, 1043
SduI GDGCHC 5 cut(s) 122, 624, 807, 1123, 1651
SfaNI GCATC 1 cut(s) 654
SfcI CTRYAG 3 cut(s) 946, 1449, 1672
Sfr274I CTCGAG 1 cut(s) 384
SinI GGWCC 2 cut(s) 1081, 1388
SlaI CTCGAG 1 cut(s) 384
SmiMI CAYNNNNRTG 4 cut(s) 600, 702, 810, 1073
SmlI CTYRAG 4 cut(s) 384, 764, 983, 1211
SmoI CTYRAG 4 cut(s) 384, 764, 983, 1211
SsiI CCGC 9 cut(s) 56, 136, 142, 181, 324, 402, 843, 1446, 1548
SspMI CTAG 2 cut(s) 311, 1652
SstI GAGCTC 1 cut(s) 624
StyI CCWWGG 3 cut(s) 499, 1221, 1384
TaaI ACNGT 5 cut(s) 412, 898, 967, 1034, 1564
TaiI ACGT 1 cut(s) 261
TaqI TCGA 5 cut(s) 8, 385, 624, 892, 1626
TatI WGTACW 4 cut(s) 412, 1016, 1041, 1755
TauI GCSGC 2 cut(s) 405, 1448
TfiI GAWTC 7 cut(s) 293, 511, 546, 939, 1337, 1623, 1772
Tru1I TTAA 7 cut(s) 288, 585, 765, 920, 1284, 1707, 1761
Tru9I TTAA 7 cut(s) 288, 585, 765, 920, 1284, 1707, 1761
TscAI CASTG 4 cut(s) 379, 415, 535, 1075
TseFI GTSAC 2 cut(s) 319, 370
TseI GCWGC 3 cut(s) 90, 93, 1448
Tsp45I GTSAC 2 cut(s) 319, 370
TspDTI ATGAA 6 cut(s) 305, 686, 720, 1596, 1704, 1785
TspRI CASTG 4 cut(s) 379, 415, 535, 1075
Tth111I GACNNNGTC 1 cut(s) 1562
Van91I CCANNNNNTGG 1 cut(s) 799
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 1081, 1388
XapI RAATTY 2 cut(s) 194, 1355
XceI RCATGY 2 cut(s) 419, 841
XcmI CCANNNNNNNNNTGG 2 cut(s) 599, 1169
XhoI CTCGAG 1 cut(s) 384
XmiI GTMKAC 2 cut(s) 820, 945
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 2 cut(s) 311, 1652
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 2 cut(s) 1018, 1043
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.