RchiOBHm_Chr4g0384801

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009675
Physical Location & Seq
Reverse (-)
144325 .. 154856
10532 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35835

Sequence Viewer

Length: 1818 bp
ATGAGAGTGGTGATGGAGTGTGCAATTCCGGGAAAGTTGCTTTGTGCTTCAACTTCAGGACTCTGGAATCCGAGGAAGGTAGTGTTGAGCAGAAGAAACACGGGACTGAGTGCCCACAACAACTTGTGCTCCTCTAAATTCAAGATTAAAGCTCAGACCCATCACACATCACAGGTCGGCATTGCAAAGCCAACAAATTTGACTGATTCTGATATTCAGGGATTTGTTTGGCCTTCTCCAACAGATGAAATCCCATTTTGGAAGAAGGAGTTCCCTTCTTGGGATGCAAACTTGGAACTTCCCTCTGAACAAACCAAGGATTCCAAACTCATGCACATTGTTCATGTTACAGCTGAAATGGCACCAATAGCAAAAGTTGGTGGTCTTGGTGATGTTGTAACTGGACTTGCTCGAGCATGTTTATCACGTGGCCACACAGTGCATATAATGCTTCCTTTCTATGAATGTATCCAGAAACATCAAATCAATGACTTGACGTTAATCACAACATATAACTCTTATCATGATGGAAACTGGATCCCCACTAATGCATATCATGGGGTAGTTTCAGGAATCCCAGTTATATTTCTTGAACCATCCTGTCAGTTCTTTAAGGGACGTCAGGTGTATGGGGGTTCATACAATGAGCTGGAGGCATATATGTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGGTGACTGGAACACAACCTGATATCATTCATGTCCATGAGTGGCAGGCTGGTGCTTTACCCATACTTTATTGGGACATGTATCATCATCTTTCACTTAAGAACCCGAGAATAGTATTGACAATCCATAACATGGAGCACTATGGTGAATGTAGACAAGAGCAACTTAGCATGTGTGGTCTTGATGGATCCCTCTATGCAACCTTTGACAAGGCAGTTGATGATCGAACTGTTGGTCACAATCCCGAACGGTTAAGCTTATTGAAAGGTGGAATTGTCTACAGCAATGCAGTAGTCACAGTTTCTCCAACATATCTCAAAGAGACACTCTGCTCTGGATGGCTTTCAAGTACTTTGATGAGAAATCGTGATAAGTACTTTGGTATAGTAAATGGAATAGACACTACGATGTGGAACCCTTCTACTGATGTTTTCTTGCCTTCGAAATTCAATGTTAAAAACTCTGAGGGCAAGAAGTTATGCAAATACTATGTTCAAAGGGGACTTGGTTTGGCCTCAGGCGACCACGTGACAGATACTACTCTCAAGGTGCCTTTGGTTGTCTGTATTACTCGCTTAGTTGCACAAAAGGGTCTTCATTTGATTACTCATGCAATCAAGCAAGTTGAAGAACTAGGTGGACAGATGGTTGTACTAGGAAAAGCCCCGGATCACCGGGTAGAAAGAGAATTTGAAGACCTTGCAGATTCGCTTAACCAAGGTCCTAGCATTCGGATTTTATTGATGTATAGTGAGGAGCTATCCCACTTACTTTATGCTGCTGCAGACATTGTTCTAGTTCCTTCCATGTATGAGCCTTGTGGACTGGCCCAAATGATTGGCATGCGTTATGGGGCAGTCCCAGTAGTTAGAAAGACTGGCGGTCTTGCAGATACAGTCTTTGACATGGATGATGAGTCAAACCATGAGATGGCAAATGGGTTTGTTTTTGAAGGAATGGACGAAGCTTCCCTGAATTGTGCTCTAGATCGTGCATTTGCTTGCTATAGAAACAAGCCAGGAGAATGGAATTGCATTGTTAAGAAGGTAATGAAGATGGATAATAGCTGGAACAACACAGCTCGCAAGTACATTGAGATTTATGAATCAGTTACAGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

605

Amino Acids

67.73

Weight (kDa)

6.45

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 112 - 340 1.2e-54 Starch synthase catalytic domain
Glyco_transf_4 PF13439 126 - 287 5.7e-08 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 414 - 566 1.4e-13 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 416 - 558 3e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 685
AatII GACGTC 1 cut(s) 622
Acc36I ACCTGC 1 cut(s) 685
AccB1I GGYRCC 2 cut(s) 361, 1243
AccB7I CCANNNNNTGG 2 cut(s) 826, 1624
AccI GTMKAC 2 cut(s) 847, 972
AciI CCGC 1 cut(s) 1575
AclWI GGATC 5 cut(s) 532, 545, 876, 889, 1371
AcoI YGGCCR 1 cut(s) 430
AcsI RAATTY 4 cut(s) 137, 196, 1139, 1382
AcuI CTGAAG 1 cut(s) 39
AcvI CACGTG 2 cut(s) 428, 1222
AcyI GRCGYC 1 cut(s) 619
AdeI CACNNNGTG 1 cut(s) 439
AfaI GTAC 4 cut(s) 1045, 1070, 1347, 1784
AfiI CCNNNNNNNGG 3 cut(s) 280, 826, 1624
AflII CTTAAG 1 cut(s) 791
AflIII ACRYGT 1 cut(s) 771
AhdI GACNNNNNGTC 1 cut(s) 1575
AjnI CCWGG 1 cut(s) 1711
AleI CACNNNNGTG 1 cut(s) 837
AluBI AGCT 8 cut(s) 152, 353, 649, 951, 1453, 1661, 1761, 1775
AluI AGCT 8 cut(s) 152, 353, 649, 951, 1453, 1661, 1761, 1775
Alw21I GWGCWC 3 cut(s) 131, 834, 1678
Alw26I GTCTC 1 cut(s) 1010
AlwI GGATC 5 cut(s) 532, 545, 876, 889, 1371
Ama87I CYCGRG 2 cut(s) 411, 799
AoxI GGCC 4 cut(s) 230, 430, 1206, 1521
ApeKI GCWGC 2 cut(s) 1472, 1475
ApoI RAATTY 4 cut(s) 137, 196, 1139, 1382
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
Asp700I GAANNNNTTC 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 1415, 1522
AsuC2I CCSGG 3 cut(s) 30, 1361, 1370
AsuHPI GGTGA 5 cut(s) 22, 401, 709, 851, 1358
AsuII TTCGAA 1 cut(s) 1136
AvaI CYCGRG 2 cut(s) 411, 799
AvaII GGWCC 1 cut(s) 1415
AxyI CCTNAGG 1 cut(s) 1210
BaeGI GKGCMC 1 cut(s) 115
BalI TGGCCA 1 cut(s) 432
BamHI GGATCC 2 cut(s) 537, 881
BanI GGYRCC 2 cut(s) 361, 1243
BbrPI CACGTG 2 cut(s) 428, 1222
BbsI GAAGAC 2 cut(s) 1280, 1395
Bbv12I GWGCWC 3 cut(s) 131, 834, 1678
BbvI GCAGC 2 cut(s) 1459, 1462
BccI CCATC 9 cut(s) 7, 168, 521, 604, 872, 1026, 1333, 1618, 1744
BciT130I CCWGG 1 cut(s) 1713
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 3 cut(s) 30, 1361, 1370
BcoDI GTCTC 1 cut(s) 1010
BfaI CTAG 5 cut(s) 1328, 1349, 1419, 1490, 1679
BfmI CTRYAG 3 cut(s) 973, 1476, 1699
BfrI CTTAAG 1 cut(s) 791
BfuAI ACCTGC 1 cut(s) 685
BfuI GTATCC 1 cut(s) 479
BisI GCNGC 2 cut(s) 1473, 1476
BlsI GCNGC 2 cut(s) 1474, 1477
BmcAI AGTACT 2 cut(s) 1045, 1070
Bme1390I CCNGG 4 cut(s) 30, 1361, 1370, 1713
Bme18I GGWCC 1 cut(s) 1415
BmeRI GACNNNNNGTC 1 cut(s) 1575
BmeT110I CYCGRG 2 cut(s) 411, 799
BmgT120I GGNCC 2 cut(s) 1415, 1522
BmiI GGNNCC 5 cut(s) 363, 539, 883, 1109, 1245
BmrFI CCNGG 4 cut(s) 30, 1361, 1370, 1713
BmrI ACTGGG 2 cut(s) 572, 1550
BmsI GCATC 2 cut(s) 274, 681
BmuI ACTGGG 2 cut(s) 572, 1550
BpiI GAAGAC 2 cut(s) 1280, 1395
BpmI CTGGAG 1 cut(s) 671
Bpu14I TTCGAA 1 cut(s) 1136
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 3 cut(s) 30, 1361, 1370
BsaAI YACGTR 2 cut(s) 428, 1222
BsaHI GRCGYC 1 cut(s) 619
BsaJI CCNNGG 4 cut(s) 71, 315, 1359, 1411
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 982, 1012
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 826, 1624
Bse1I ACTGG 7 cut(s) 406, 539, 578, 706, 1524, 1556, 1576
Bse21I CCTNAGG 1 cut(s) 1210
Bse3DI GCAATG 2 cut(s) 180, 985
BseBI CCWGG 1 cut(s) 1713
BseDI CCNNGG 4 cut(s) 71, 315, 1359, 1411
BseGI GGATG 5 cut(s) 289, 596, 696, 1037, 1609
BseLI CCNNNNNNNGG 3 cut(s) 280, 826, 1624
BseMI GCAATG 2 cut(s) 180, 985
BseMII CTCAG 4 cut(s) 98, 167, 1149, 1224
BseNI ACTGG 7 cut(s) 406, 539, 578, 706, 1524, 1556, 1576
BseRI GAGGAG 2 cut(s) 121, 1463
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 1459, 1462
BshFI GGCC 4 cut(s) 232, 432, 1208, 1523
BshNI GGYRCC 2 cut(s) 361, 1243
BsiHKAI GWGCWC 3 cut(s) 131, 834, 1678
BsiHKCI CYCGRG 2 cut(s) 411, 799
BsiSI CCGG 3 cut(s) 29, 1361, 1369
BslFI GGGAC 5 cut(s) 117, 630, 782, 1209, 1538
BslI CCNNNNNNNGG 3 cut(s) 280, 826, 1624
BsmAI GTCTC 1 cut(s) 1010
BsmFI GGGAC 5 cut(s) 117, 630, 782, 1209, 1538
BsmI GAATGC 1 cut(s) 1422
BsnI GGCC 4 cut(s) 232, 432, 1208, 1523
BsoBI CYCGRG 2 cut(s) 411, 799
Bsp119I TTCGAA 1 cut(s) 1136
Bsp1286I GDGCHC 4 cut(s) 115, 131, 834, 1678
Bsp143I GATC 5 cut(s) 537, 881, 916, 1363, 1681
BspACI CCGC 1 cut(s) 1575
BspANI GGCC 4 cut(s) 232, 432, 1208, 1523
BspCNI CTCAG 4 cut(s) 99, 166, 1150, 1223
BspHI TCATGA 2 cut(s) 523, 1814
BspLI GGNNCC 5 cut(s) 363, 539, 883, 1109, 1245
BspMAI CTGCAG 1 cut(s) 1480
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 5 cut(s) 532, 545, 876, 889, 1371
BspT104I TTCGAA 1 cut(s) 1136
BspT107I GGYRCC 2 cut(s) 361, 1243
BspTI CTTAAG 1 cut(s) 791
BsrDI GCAATG 2 cut(s) 180, 985
BsrI ACTGG 7 cut(s) 406, 539, 578, 706, 1524, 1556, 1576
BssECI CCNNGG 4 cut(s) 71, 315, 1359, 1411
BssMI GATC 5 cut(s) 537, 881, 916, 1363, 1681
BssNI GRCGYC 1 cut(s) 619
BssT1I CCWWGG 2 cut(s) 315, 1411
Bst2UI CCWGG 1 cut(s) 1713
Bst4CI ACNGT 5 cut(s) 439, 925, 945, 994, 1591
BstACI GRCGYC 1 cut(s) 619
BstAFI CTTAAG 1 cut(s) 791
BstAPI GCANNNNNTGC 1 cut(s) 448
BstBAI YACGTR 2 cut(s) 428, 1222
BstBI TTCGAA 1 cut(s) 1136
BstC8I GCNNGC 5 cut(s) 679, 741, 1538, 1696, 1777
BstDEI CTNAG 6 cut(s) 107, 153, 860, 1158, 1210, 1270
BstF5I GGATG 5 cut(s) 289, 596, 696, 1037, 1609
BstKTI GATC 5 cut(s) 540, 884, 919, 1366, 1684
BstMAI GTCTC 1 cut(s) 1010
BstMBI GATC 5 cut(s) 537, 881, 916, 1363, 1681
BstMWI GCNNNNNNNGC 3 cut(s) 359, 368, 448
BstNI CCWGG 1 cut(s) 1713
BstNSI RCATGY 4 cut(s) 420, 775, 868, 1540
BstSCI CCNGG 4 cut(s) 28, 1359, 1368, 1711
BstSFI CTRYAG 3 cut(s) 973, 1476, 1699
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 1459, 1462
BstV2I GAAGAC 2 cut(s) 1280, 1395
BstX2I RGATCY 2 cut(s) 537, 881
BstXI CCANNNNNNTGG 2 cut(s) 1532, 1719
BstYI RGATCY 2 cut(s) 537, 881
Bsu36I CCTNAGG 1 cut(s) 1210
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 4 cut(s) 232, 432, 1208, 1523
BtsCI GGATG 5 cut(s) 289, 596, 696, 1037, 1609
BtsIMutI CAGTG 1 cut(s) 444
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 5 cut(s) 679, 741, 1538, 1696, 1777
CciI TCATGA 2 cut(s) 523, 1814
Cfr13I GGNCC 2 cut(s) 1415, 1522
Csp6I GTAC 4 cut(s) 1044, 1069, 1346, 1783
CviQI GTAC 4 cut(s) 1044, 1069, 1346, 1783
DdeI CTNAG 6 cut(s) 107, 153, 860, 1158, 1210, 1270
DpnI GATC 5 cut(s) 539, 883, 918, 1365, 1683
DpnII GATC 5 cut(s) 537, 881, 916, 1363, 1681
DraIII CACNNNGTG 1 cut(s) 439
DriI GACNNNNNGTC 1 cut(s) 1575
EaeI YGGCCR 1 cut(s) 430
Eam1105I GACNNNNNGTC 1 cut(s) 1575
Eco130I CCWWGG 2 cut(s) 315, 1411
Eco32I GATATC 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 1415
Eco57I CTGAAG 1 cut(s) 39
Eco72I CACGTG 2 cut(s) 428, 1222
Eco81I CCTNAGG 1 cut(s) 1210
Eco88I CYCGRG 2 cut(s) 411, 799
EcoO109I RGGNCCY 1 cut(s) 1415
EcoRII CCWGG 1 cut(s) 1711
EcoRV GATATC 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 315, 1411
EcoT22I ATGCAT 1 cut(s) 553
ErhI CCWWGG 2 cut(s) 315, 1411
FalI AAGNNNNNCTT 2 cut(s) 843, 875
FaqI GGGAC 5 cut(s) 117, 630, 782, 1209, 1538
FblI GTMKAC 2 cut(s) 847, 972
Fnu4HI GCNGC 2 cut(s) 1473, 1476
FokI GGATG 5 cut(s) 296, 583, 703, 1044, 1616
Fsp4HI GCNGC 2 cut(s) 1473, 1476
FspBI CTAG 5 cut(s) 1328, 1349, 1419, 1490, 1679
GluI GCNGC 2 cut(s) 1473, 1476
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 4 cut(s) 232, 432, 1208, 1523
HapII CCGG 3 cut(s) 29, 1361, 1369
Hin1I GRCGYC 1 cut(s) 619
HindIII AAGCTT 2 cut(s) 949, 1659
HinfI GANTC 8 cut(s) 60, 67, 206, 320, 573, 1400, 1610, 1799
HpaII CCGG 3 cut(s) 29, 1361, 1369
HphI GGTGA 5 cut(s) 22, 401, 709, 851, 1358
Hpy166II GTNNAC 4 cut(s) 848, 973, 1334, 1517
Hpy188I TCNGA 6 cut(s) 72, 156, 211, 307, 1159, 1428
Hpy8I GTNNAC 4 cut(s) 848, 973, 1334, 1517
HpyAV CCTTC 9 cut(s) 70, 243, 259, 285, 1122, 1143, 1506, 1640, 1732
HpyCH4III ACNGT 5 cut(s) 439, 925, 945, 994, 1591
HpyCH4IV ACGT 4 cut(s) 427, 497, 619, 1221
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 368, 448
HpyF3I CTNAG 6 cut(s) 107, 153, 860, 1158, 1210, 1270
HpySE526I ACGT 4 cut(s) 427, 497, 619, 1221
Hsp92I GRCGYC 1 cut(s) 619
Kzo9I GATC 5 cut(s) 537, 881, 916, 1363, 1681
LmnI GCTCC 3 cut(s) 134, 829, 1450
Lsp1109I GCAGC 2 cut(s) 1459, 1462
LweI GCATC 2 cut(s) 274, 681
MaeI CTAG 5 cut(s) 1328, 1349, 1419, 1490, 1679
MaeII ACGT 4 cut(s) 427, 497, 619, 1221
MaeIII GTNAC 7 cut(s) 346, 397, 697, 929, 988, 1222, 1804
MalI GATC 5 cut(s) 539, 883, 918, 1365, 1683
MboI GATC 5 cut(s) 537, 881, 916, 1363, 1681
MboII GAAGA 6 cut(s) 105, 274, 1280, 1334, 1400, 1759
MflI RGATCY 2 cut(s) 537, 881
MhlI GDGCHC 4 cut(s) 115, 131, 834, 1678
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 8 cut(s) 24, 137, 196, 966, 1139, 1382, 1669, 1723
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 2 cut(s) 54, 1619
MmeI TCCRAC 2 cut(s) 263, 1025
MnlI CCTC 8 cut(s) 66, 142, 313, 646, 896, 1153, 1219, 1441
Mox20I TGGCCA 1 cut(s) 432
Mph1103I ATGCAT 1 cut(s) 553
MroXI GAANNNNTTC 1 cut(s) 269
MscI TGGCCA 1 cut(s) 432
MseI TTAA 8 cut(s) 147, 500, 612, 792, 947, 1149, 1407, 1734
MslI CAYNNNNRTG 3 cut(s) 729, 837, 1100
Msp20I TGGCCA 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 353
MspCI CTTAAG 1 cut(s) 791
MspI CCGG 3 cut(s) 29, 1361, 1369
MspR9I CCNGG 4 cut(s) 30, 1361, 1370, 1713
Mva1269I GAATGC 1 cut(s) 1422
MvaI CCWGG 1 cut(s) 1713
MwoI GCNNNNNNNGC 3 cut(s) 359, 368, 448
NciI CCSGG 3 cut(s) 30, 1361, 1370
NdeII GATC 5 cut(s) 537, 881, 916, 1363, 1681
NlaIV GGNNCC 5 cut(s) 363, 539, 883, 1109, 1245
NmuCI GTSAC 4 cut(s) 697, 929, 988, 1222
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 4 cut(s) 420, 775, 868, 1540
NspV TTCGAA 1 cut(s) 1136
OliI CACNNNNGTG 1 cut(s) 837
PaeI GCATGC 1 cut(s) 1540
PaeR7I CTCGAG 1 cut(s) 411
PagI TCATGA 2 cut(s) 523, 1814
PaqCI CACCTGC 1 cut(s) 685
PciI ACATGT 1 cut(s) 771
PctI GAATGC 1 cut(s) 1422
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 6 cut(s) 67, 206, 320, 573, 1400, 1799
PflMI CCANNNNNTGG 2 cut(s) 826, 1624
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 2 cut(s) 1474, 1477
PleI GAGTC 2 cut(s) 54, 1618
PmaCI CACGTG 2 cut(s) 428, 1222
PmlI CACGTG 2 cut(s) 428, 1222
PpsI GAGTC 2 cut(s) 54, 1618
Ppu21I YACGTR 2 cut(s) 428, 1222
PpuMI RGGWCCY 1 cut(s) 1415
PscI ACATGT 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 1415
Psp6I CCWGG 1 cut(s) 1711
PspCI CACGTG 2 cut(s) 428, 1222
PspGI CCWGG 1 cut(s) 1711
PspN4I GGNNCC 5 cut(s) 363, 539, 883, 1109, 1245
PspPI GGNCC 2 cut(s) 1415, 1522
PspPPI RGGWCCY 1 cut(s) 1415
PspXI VCTCGAGB 1 cut(s) 411
PstI CTGCAG 1 cut(s) 1480
PsuI RGATCY 2 cut(s) 537, 881
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 4 cut(s) 1045, 1070, 1347, 1784
RsaNI GTAC 4 cut(s) 1044, 1069, 1346, 1783
RseI CAYNNNNRTG 3 cut(s) 729, 837, 1100
SaqAI TTAA 8 cut(s) 147, 500, 612, 792, 947, 1149, 1407, 1734
SatI GCNGC 2 cut(s) 1473, 1476
Sau3AI GATC 5 cut(s) 537, 881, 916, 1363, 1681
Sau96I GGNCC 2 cut(s) 1415, 1522
ScaI AGTACT 2 cut(s) 1045, 1070
SchI GAGTC 2 cut(s) 54, 1619
ScrFI CCNGG 4 cut(s) 30, 1361, 1370, 1713
SduI GDGCHC 4 cut(s) 115, 131, 834, 1678
SfaNI GCATC 2 cut(s) 274, 681
SfcI CTRYAG 3 cut(s) 973, 1476, 1699
Sfr274I CTCGAG 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 1136
SinI GGWCC 1 cut(s) 1415
SlaI CTCGAG 1 cut(s) 411
SmiMI CAYNNNNRTG 3 cut(s) 729, 837, 1100
SmlI CTYRAG 3 cut(s) 411, 791, 1238
SmoI CTYRAG 3 cut(s) 411, 791, 1238
SphI GCATGC 1 cut(s) 1540
Sse9I AATT 8 cut(s) 24, 137, 196, 966, 1139, 1382, 1669, 1723
SsiI CCGC 1 cut(s) 1575
SspMI CTAG 5 cut(s) 1328, 1349, 1419, 1490, 1679
StyD4I CCNGG 4 cut(s) 28, 1359, 1368, 1711
StyI CCWWGG 2 cut(s) 315, 1411
TaaI ACNGT 5 cut(s) 439, 925, 945, 994, 1591
TaiI ACGT 4 cut(s) 430, 500, 622, 1224
TaqI TCGA 3 cut(s) 412, 919, 1136
TasI AATT 8 cut(s) 24, 137, 196, 966, 1139, 1382, 1669, 1723
TatI WGTACW 4 cut(s) 1043, 1068, 1345, 1782
TfiI GAWTC 6 cut(s) 67, 206, 320, 573, 1400, 1799
Tru1I TTAA 8 cut(s) 147, 500, 612, 792, 947, 1149, 1407, 1734
Tru9I TTAA 8 cut(s) 147, 500, 612, 792, 947, 1149, 1407, 1734
TscAI CASTG 1 cut(s) 444
TseFI GTSAC 4 cut(s) 697, 929, 988, 1222
TseI GCWGC 2 cut(s) 1472, 1475
Tsp45I GTSAC 4 cut(s) 697, 929, 988, 1222
TspDTI ATGAA 8 cut(s) 261, 332, 477, 627, 713, 1280, 1760, 1812
TspRI CASTG 1 cut(s) 444
Van91I CCANNNNNTGG 2 cut(s) 826, 1624
Vha464I CTTAAG 1 cut(s) 791
VpaK11BI GGWCC 1 cut(s) 1415
XapI RAATTY 4 cut(s) 137, 196, 1139, 1382
XbaI TCTAGA 1 cut(s) 1678
XceI RCATGY 4 cut(s) 420, 775, 868, 1540
XhoI CTCGAG 1 cut(s) 411
XmiI GTMKAC 2 cut(s) 847, 972
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 5 cut(s) 1328, 1349, 1419, 1490, 1679
ZraI GACGTC 1 cut(s) 620
ZrmI AGTACT 2 cut(s) 1045, 1070
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.