Rh4DG001400

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009675
Physical Location & Seq
Reverse (-)
289991 .. 300762
10772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG001400.1

Sequence Viewer

Length: 1449 bp
ATGAGAGTGGTGATGATGGAGTGTGCAATTCCGGGAAAGTTGCTTTGTGCTTCAACTTCAAGACTCTGGAATCCGAGGCCGAGGAAGGTAGTGTTGAGCAGAAGAAACACGGGACTGAGTGCCCACAGCAACAACTTGTGCAGCTCCTCTAAATTCAAGATTAAAGCTCACACATCACAGGTCGGCATTGCAAAGCCAACAAATTTGACTGATTCTGATATTCAGGGATTTGTTTGGCCTTCTCCAACAGATGAAATCCCATTTTGGAAGAAGGAGTTCCCTTCTTGGGATGCAAACTTGGAACTTCCCTCTGAACAAACCAAGGATTCCAAACTCATGCACATTGTTCATGTTACAGCTGAAATGGCACCAATAGCAAAAGTTGGTGGTCTTGGTGATGTTGTAACTGGACTTGCTCGAGCATGTTTATCACGTGGCCACACAGTGCATATAATGCTTCCTTTCTATGAGTGTATCCAGAAACATCAAATCAATGACTTGACGTTAATCACAACATATAACTCTTATCATGATGGAAACTGGATCCCCACTAATGCATATCATGGGGTAGTTTCAGGAATCCCAGTTATATTTCTTGAACCATCCTGTCAGTTCTTTAAGGGACATCAGGTGTATGGGGGTCCATACAATGAGCTGGAGGCATATATGTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGGTGACTGGAACACAACCTGATATCATTCATGTCCATGAGTGGCAGGCTGGTGCTTTACCCATACTTTATTGGGACATGTATCATCATCTTTCACTTAAGAAGCCGAGAATAGTATTGACAATCCATAACATGGAGCACTATGGTGAATGTAGACAAGAGCAACTTAGCATGTGTGGTCTTGATGGATCCCTCTATGCAACCTTTGACAAGGCAGTTGATGATCGAACTGTTGGTCACAATCCCGAACGGTTAAGCTTATTGAAAGGTGGAATTGTCTACAGCAATGCAGTAGTCACAGTTTCTCCAACATATCTCAAAGAGACACTCTGCTCTGGATGGCTTTCAAGTACTTTGATGAGAAATCGTGATAAGTACTTTGGTATAGTAAATGGAATAGACACTACAATGTGGAACCCTTCTACTGATGTTTTCTTGCCTTCGAAATTCAATGTTAAAAACTCTGAGGGCAAGAAGTTATGCAAATACTATGTTCAAAGGGGACTTGGTTTGGCCTCAGGAGACCACGTGACAGATACTACTCTCAAGGTGCCTTTGGTTGTCTGTATTACTCGCTTAGTTGCACAAAAGGGTCTTCATTTGATTACTCATGCAATCAAGCAAGTTGAAGAACTAGGTGGACAGATGGTTGTACTGGGAAAAGCCCCGGATCACCGGGTTGAAAGAGAATTTGAAGACCTTGCAGATTCGGTATTGGATTTAAGATTTATGATTTTTGGTTTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

482

Amino Acids

54.12

Weight (kDa)

7.92

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 114 - 342 1e-54 Starch synthase catalytic domain
Glyco_transf_4 PF13439 128 - 289 2.7e-08 Glycosyltransferase Family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 691
Acc36I ACCTGC 1 cut(s) 691
AccB1I GGYRCC 2 cut(s) 367, 1249
AccB7I CCANNNNNTGG 1 cut(s) 832
AccI GTMKAC 2 cut(s) 853, 978
AclWI GGATC 5 cut(s) 538, 551, 882, 895, 1377
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 4 cut(s) 152, 202, 1145, 1388
AcvI CACGTG 2 cut(s) 434, 1228
AdeI CACNNNGTG 1 cut(s) 445
AfaI GTAC 3 cut(s) 1051, 1076, 1353
AfiI CCNNNNNNNGG 2 cut(s) 286, 832
AflII CTTAAG 1 cut(s) 797
AflIII ACRYGT 1 cut(s) 777
AleI CACNNNNGTG 1 cut(s) 843
AluBI AGCT 5 cut(s) 144, 167, 359, 655, 957
AluI AGCT 5 cut(s) 144, 167, 359, 655, 957
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 2 cut(s) 1016, 1215
AlwI GGATC 5 cut(s) 538, 551, 882, 895, 1377
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 4 cut(s) 77, 236, 436, 1212
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 4 cut(s) 152, 202, 1145, 1388
ArsI GACNNNNNNTTYG 2 cut(s) 919, 951
Asp700I GAANNNNTTC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 641
AsuC2I CCSGG 3 cut(s) 33, 1367, 1376
AsuHPI GGTGA 5 cut(s) 22, 407, 715, 857, 1364
AsuII TTCGAA 1 cut(s) 1142
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 1216
BaeGI GKGCMC 1 cut(s) 124
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 2 cut(s) 543, 887
BanI GGYRCC 2 cut(s) 367, 1249
BbrPI CACGTG 2 cut(s) 434, 1228
BbsI GAAGAC 2 cut(s) 1286, 1401
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 1 cut(s) 153
BccI CCATC 6 cut(s) 10, 527, 610, 878, 1032, 1339
BciVI GTATCC 1 cut(s) 485
BcnI CCSGG 3 cut(s) 33, 1367, 1376
BcoDI GTCTC 2 cut(s) 1016, 1215
BfaI CTAG 2 cut(s) 1334, 1447
BfmI CTRYAG 1 cut(s) 979
BfrI CTTAAG 1 cut(s) 797
BfuAI ACCTGC 1 cut(s) 691
BfuI GTATCC 1 cut(s) 485
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
BmcAI AGTACT 2 cut(s) 1051, 1076
Bme1390I CCNGG 3 cut(s) 33, 1367, 1376
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 641
BmiI GGNNCC 6 cut(s) 369, 545, 642, 889, 1115, 1251
BmrFI CCNGG 3 cut(s) 33, 1367, 1376
BmrI ACTGGG 2 cut(s) 578, 1364
BmsI GCATC 2 cut(s) 280, 687
BmuI ACTGGG 2 cut(s) 578, 1364
BpiI GAAGAC 2 cut(s) 1286, 1401
BpmI CTGGAG 1 cut(s) 677
Bpu14I TTCGAA 1 cut(s) 1142
BpuEI CTTGAG 1 cut(s) 1229
BpuMI CCSGG 3 cut(s) 33, 1367, 1376
BsaAI YACGTR 2 cut(s) 434, 1228
BsaI GGTCTC 1 cut(s) 1215
BsaJI CCNNGG 4 cut(s) 74, 80, 321, 1365
BsaXI ACNNNNNCTCC 4 cut(s) 988, 1018, 1212, 1242
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 832
Bse1I ACTGG 5 cut(s) 412, 545, 584, 712, 1359
Bse21I CCTNAGG 1 cut(s) 1216
Bse3DI GCAATG 2 cut(s) 186, 991
BseDI CCNNGG 4 cut(s) 74, 80, 321, 1365
BseGI GGATG 4 cut(s) 295, 602, 702, 1043
BseLI CCNNNNNNNGG 2 cut(s) 286, 832
BseMI GCAATG 2 cut(s) 186, 991
BseMII CTCAG 3 cut(s) 107, 1155, 1230
BseNI ACTGG 5 cut(s) 412, 545, 584, 712, 1359
BseRI GAGGAG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 1 cut(s) 153
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 4 cut(s) 79, 238, 438, 1214
BshNI GGYRCC 2 cut(s) 367, 1249
BsiHKAI GWGCWC 1 cut(s) 840
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 3 cut(s) 32, 1367, 1375
BslFI GGGAC 4 cut(s) 126, 636, 788, 1215
BslI CCNNNNNNNGG 2 cut(s) 286, 832
BsmAI GTCTC 2 cut(s) 1016, 1215
BsmFI GGGAC 4 cut(s) 126, 636, 788, 1215
BsnI GGCC 4 cut(s) 79, 238, 438, 1214
Bso31I GGTCTC 1 cut(s) 1215
BsoBI CYCGRG 1 cut(s) 417
Bsp119I TTCGAA 1 cut(s) 1142
Bsp1286I GDGCHC 2 cut(s) 124, 840
Bsp143I GATC 4 cut(s) 543, 887, 922, 1369
BspANI GGCC 4 cut(s) 79, 238, 438, 1214
BspCNI CTCAG 3 cut(s) 108, 1156, 1229
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 6 cut(s) 369, 545, 642, 889, 1115, 1251
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 5 cut(s) 538, 551, 882, 895, 1377
BspT104I TTCGAA 1 cut(s) 1142
BspT107I GGYRCC 2 cut(s) 367, 1249
BspTI CTTAAG 1 cut(s) 797
BspTNI GGTCTC 1 cut(s) 1215
BsrDI GCAATG 2 cut(s) 186, 991
BsrI ACTGG 5 cut(s) 412, 545, 584, 712, 1359
BssECI CCNNGG 4 cut(s) 74, 80, 321, 1365
BssMI GATC 4 cut(s) 543, 887, 922, 1369
BssT1I CCWWGG 1 cut(s) 321
Bst4CI ACNGT 4 cut(s) 445, 931, 951, 1000
BstAFI CTTAAG 1 cut(s) 797
BstAPI GCANNNNNTGC 1 cut(s) 454
BstBAI YACGTR 2 cut(s) 434, 1228
BstBI TTCGAA 1 cut(s) 1142
BstC8I GCNNGC 2 cut(s) 685, 747
BstDEI CTNAG 5 cut(s) 116, 866, 1164, 1216, 1276
BstF5I GGATG 4 cut(s) 295, 602, 702, 1043
BstKTI GATC 4 cut(s) 546, 890, 925, 1372
BstMAI GTCTC 2 cut(s) 1016, 1215
BstMBI GATC 4 cut(s) 543, 887, 922, 1369
BstMWI GCNNNNNNNGC 3 cut(s) 365, 374, 454
BstNSI RCATGY 3 cut(s) 426, 781, 874
BstSCI CCNGG 3 cut(s) 31, 1365, 1374
BstSFI CTRYAG 1 cut(s) 979
BstSLI GKGCMC 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 153
BstV2I GAAGAC 2 cut(s) 1286, 1401
BstX2I RGATCY 2 cut(s) 543, 887
BstYI RGATCY 2 cut(s) 543, 887
Bsu36I CCTNAGG 1 cut(s) 1216
BsuI GTATCC 1 cut(s) 485
BsuRI GGCC 4 cut(s) 79, 238, 438, 1214
BtsCI GGATG 4 cut(s) 295, 602, 702, 1043
BtsIMutI CAGTG 1 cut(s) 450
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 685, 747
CciI TCATGA 1 cut(s) 529
Cfr13I GGNCC 1 cut(s) 641
Csp6I GTAC 3 cut(s) 1050, 1075, 1352
CviQI GTAC 3 cut(s) 1050, 1075, 1352
DdeI CTNAG 5 cut(s) 116, 866, 1164, 1216, 1276
DpnI GATC 4 cut(s) 545, 889, 924, 1371
DpnII GATC 4 cut(s) 543, 887, 922, 1369
DraIII CACNNNGTG 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 1215
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 641
Eco72I CACGTG 2 cut(s) 434, 1228
Eco81I CCTNAGG 1 cut(s) 1216
Eco88I CYCGRG 1 cut(s) 417
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 321
EcoT22I ATGCAT 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 321
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 4 cut(s) 126, 636, 788, 1215
FblI GTMKAC 2 cut(s) 853, 978
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 4 cut(s) 302, 589, 709, 1050
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 2 cut(s) 1334, 1447
GluI GCNGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 677
HaeIII GGCC 4 cut(s) 79, 238, 438, 1214
HapII CCGG 3 cut(s) 32, 1367, 1375
HindIII AAGCTT 1 cut(s) 955
HinfI GANTC 6 cut(s) 63, 70, 212, 326, 579, 1406
HpaII CCGG 3 cut(s) 32, 1367, 1375
HphI GGTGA 5 cut(s) 22, 407, 715, 857, 1364
Hpy166II GTNNAC 3 cut(s) 854, 979, 1340
Hpy188I TCNGA 4 cut(s) 75, 217, 313, 1165
Hpy8I GTNNAC 3 cut(s) 854, 979, 1340
HpyAV CCTTC 6 cut(s) 79, 249, 265, 291, 1128, 1149
HpyCH4III ACNGT 4 cut(s) 445, 931, 951, 1000
HpyCH4IV ACGT 3 cut(s) 433, 503, 1227
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 374, 454
HpyF3I CTNAG 5 cut(s) 116, 866, 1164, 1216, 1276
HpySE526I ACGT 3 cut(s) 433, 503, 1227
Kzo9I GATC 4 cut(s) 543, 887, 922, 1369
LmnI GCTCC 2 cut(s) 149, 835
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 2 cut(s) 280, 687
MaeI CTAG 2 cut(s) 1334, 1447
MaeII ACGT 3 cut(s) 433, 503, 1227
MaeIII GTNAC 6 cut(s) 352, 403, 703, 935, 994, 1228
MalI GATC 4 cut(s) 545, 889, 924, 1371
MboI GATC 4 cut(s) 543, 887, 922, 1369
MboII GAAGA 5 cut(s) 114, 280, 1286, 1340, 1406
MflI RGATCY 2 cut(s) 543, 887
MhlI GDGCHC 2 cut(s) 124, 840
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 6 cut(s) 27, 152, 202, 972, 1145, 1388
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 57
MmeI TCCRAC 2 cut(s) 269, 1031
MnlI CCTC 8 cut(s) 69, 75, 157, 319, 652, 902, 1159, 1225
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 275
MscI TGGCCA 1 cut(s) 438
MseI TTAA 7 cut(s) 162, 506, 618, 798, 953, 1155, 1421
MslI CAYNNNNRTG 3 cut(s) 735, 843, 1106
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 359
MspCI CTTAAG 1 cut(s) 797
MspI CCGG 3 cut(s) 32, 1367, 1375
MspR9I CCNGG 3 cut(s) 33, 1367, 1376
MwoI GCNNNNNNNGC 3 cut(s) 365, 374, 454
NciI CCSGG 3 cut(s) 33, 1367, 1376
NdeII GATC 4 cut(s) 543, 887, 922, 1369
NlaIV GGNNCC 6 cut(s) 369, 545, 642, 889, 1115, 1251
NmeAIII GCCGAG 2 cut(s) 105, 831
NmuCI GTSAC 4 cut(s) 703, 935, 994, 1228
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 3 cut(s) 426, 781, 874
NspV TTCGAA 1 cut(s) 1142
OliI CACNNNNGTG 1 cut(s) 843
PaeR7I CTCGAG 1 cut(s) 417
PagI TCATGA 1 cut(s) 529
PaqCI CACCTGC 1 cut(s) 691
PciI ACATGT 1 cut(s) 777
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 5 cut(s) 70, 212, 326, 579, 1406
PflMI CCANNNNNTGG 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 31
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 1 cut(s) 57
PmaCI CACGTG 2 cut(s) 434, 1228
PmlI CACGTG 2 cut(s) 434, 1228
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 2 cut(s) 434, 1228
PscI ACATGT 1 cut(s) 777
PspCI CACGTG 2 cut(s) 434, 1228
PspN4I GGNNCC 6 cut(s) 369, 545, 642, 889, 1115, 1251
PspPI GGNCC 1 cut(s) 641
PspXI VCTCGAGB 1 cut(s) 417
PsuI RGATCY 2 cut(s) 543, 887
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 1051, 1076, 1353
RsaNI GTAC 3 cut(s) 1050, 1075, 1352
RseI CAYNNNNRTG 3 cut(s) 735, 843, 1106
SaqAI TTAA 7 cut(s) 162, 506, 618, 798, 953, 1155, 1421
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 4 cut(s) 543, 887, 922, 1369
Sau96I GGNCC 1 cut(s) 641
ScaI AGTACT 2 cut(s) 1051, 1076
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 3 cut(s) 33, 1367, 1376
SduI GDGCHC 2 cut(s) 124, 840
SfaNI GCATC 2 cut(s) 280, 687
SfcI CTRYAG 1 cut(s) 979
Sfr274I CTCGAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 1142
SinI GGWCC 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 3 cut(s) 735, 843, 1106
SmlI CTYRAG 3 cut(s) 417, 797, 1244
SmoI CTYRAG 3 cut(s) 417, 797, 1244
Sse9I AATT 6 cut(s) 27, 152, 202, 972, 1145, 1388
SspMI CTAG 2 cut(s) 1334, 1447
StyD4I CCNGG 3 cut(s) 31, 1365, 1374
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 4 cut(s) 445, 931, 951, 1000
TaiI ACGT 3 cut(s) 436, 506, 1230
TaqI TCGA 3 cut(s) 418, 925, 1142
TasI AATT 6 cut(s) 27, 152, 202, 972, 1145, 1388
TatI WGTACW 3 cut(s) 1049, 1074, 1351
TfiI GAWTC 5 cut(s) 70, 212, 326, 579, 1406
Tru1I TTAA 7 cut(s) 162, 506, 618, 798, 953, 1155, 1421
Tru9I TTAA 7 cut(s) 162, 506, 618, 798, 953, 1155, 1421
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 4 cut(s) 703, 935, 994, 1228
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 4 cut(s) 703, 935, 994, 1228
TspDTI ATGAA 4 cut(s) 267, 338, 719, 1286
TspRI CASTG 1 cut(s) 450
Van91I CCANNNNNTGG 1 cut(s) 832
Vha464I CTTAAG 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 4 cut(s) 152, 202, 1145, 1388
XceI RCATGY 3 cut(s) 426, 781, 874
XhoI CTCGAG 1 cut(s) 417
XmiI GTMKAC 2 cut(s) 853, 978
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 2 cut(s) 1334, 1447
ZrmI AGTACT 2 cut(s) 1051, 1076
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.