Rw4G000750

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009675
Physical Location & Seq
Forward (+)
1514747 .. 1524936
10190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000750.1

Sequence Viewer

Length: 1830 bp
ATGAGAGTGGTGATGGAGTGTGCGATTCCGGGAAAGTTGCTTTGTGCTTCAACTTCATCAAGACTCTGGAATCCACTTCCAAGGAAGGTGGTAGTGTTGAGCAGAAGAAACACGGGACTGAGTGCCCACAACAACTTGCGCTCCTCTAAATTCAAGATTAAAGCTCAGACCCTTCACACATCACAGGTCGGCATTGCAAAGCCAACAAATTTGACTGATTCTGATATTCAGGGATTTGTTTGGCCTTCTCCAACAGATGAAATCCCATTTTGGAAGAAGGAGTTCCCTTCTTGGGATGCAAACTTGGAACTTCCCTCTGAACAAACCAAGGATTCCAAACTCATGCACATTGTTCATGTTACAGCTGAAATGGCACCAATAGCAAAAGTTGGTGGTCTTGGTGATGTTGTAACTGGACTTGCTCGAGCATGTTTATCACGTGGCCACACAGTCCATATAATGCTTCCTTTCTATGAGTGTATCCAGAAACATCAAATCAATGACTTGACGTTAATCACAACATATAACTCTTATCATGATGGAAACTGGATCCCCACTAATGCATATCATGGGGTAGTTTCAGGAATCCCAGTTATATTTCTTGAACCATCCTGTCAGTTCTTTAAGGGACGTCAGGTGTATGGGGGTTCATACAATGAGCTGGAGGCATATATGTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGGTGACTGGAACACAACCTGATATCATTCATGTCCATGAGTGGCAGGCTGGTGCTTTACCCATACTTTATTGGGACATGTATCATCATCTTTCACTTAAGAAGCCGAGAATAGTATTGACTATCCATAACATGGAGCACTATGGTGAATGTAGACAAGAGCAACTTAGCATGTGTGGTCTTGATGGATCCCTCTATGCAACCTTTGACAAGGCAGTTGATGATCGAACTGTTGGTCACAATCCCGAACGGTTAAGCTTATTGAAAGGTGGAATTGTCTACAGCAATGCAGTAGTCACAGTTTCTCCAACATATCTCAAAGAGACACTCTGCTCTGGATGGCTTTCAAGTACTTTAATGAGAAATCGTGATAAGTACTTTGGTATAGTAAATGGAATAGACACTACGATGTGGAACCCTTCTACTGATGTTTTCTTGCCTTCGAAATTCAATGTTAAAAACTCTGAGGGCAAGAAGTTATGCAAATACTATGTTCAAAGGGGACTTGGTTTGGCCTCAGGCAACCACGTGACAGATACTACTCTCAAGGTGCCTTTGGTTGTCTGTATTACTCGCTTAGTTGCACAAAAGGGTCTTCATTTGATTACTCATGCAATCAAGCAAGTTGAAGAACTAGGTGGACAGATGGTTGTACTAGGAAAGTCCCCGGATCACCGGATTGAAAGAGAATTTGAAGACCTTGCAGATTCGCTTAACCAAGGTCCTAGCATTCGGATTTTATTGATGTATAGTGAGGAGCTATCCCACTTACTTTATGCTGCTGCAGACATTGTTCTAGTTCCTTCCATGTATGAGCCATGTGGACTGGCCCAAATGATTGGCATGCGTTATGGGGCAGTCCCAGTAGTTAGAAAGACTGGTGGTCTTGCAGATACAGTCTTTGACATGGATGACGAGTCAAACCATGAGATGGCAAATGGGTTTGTTTTTGAAGGAATGGATGAAGCTTCCCTGAATTGTGCTCTAGATCGTGCATTTGCTTGCTATAGAAACAATCCAGGAGAATGGAATTGCATTGTTAAGAAGGTAATGAAGATGGATAATAGCTGGAACAACACAGCTCGCAAGTACATTGAGATTTATGAATCAGTTACAGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

609

Amino Acids

68.28

Weight (kDa)

6.74

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 116 - 344 1.4e-54 Starch synthase catalytic domain
Glyco_transf_4 PF13439 130 - 291 7.1e-08 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 418 - 569 3.3e-13 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 420 - 562 2.8e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 697
AatII GACGTC 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 697
AccB1I GGYRCC 2 cut(s) 373, 1255
AccB7I CCANNNNNTGG 2 cut(s) 838, 1636
AccI GTMKAC 2 cut(s) 859, 984
AclWI GGATC 5 cut(s) 544, 557, 888, 901, 1383
AcoI YGGCCR 1 cut(s) 442
AcsI RAATTY 4 cut(s) 149, 208, 1151, 1394
AcvI CACGTG 2 cut(s) 440, 1234
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 4 cut(s) 1057, 1082, 1359, 1796
AfiI CCNNNNNNNGG 3 cut(s) 292, 838, 1636
AflII CTTAAG 1 cut(s) 803
AflIII ACRYGT 1 cut(s) 783
AhdI GACNNNNNGTC 1 cut(s) 1587
AjnI CCWGG 1 cut(s) 1723
AleI CACNNNNGTG 1 cut(s) 849
AluBI AGCT 8 cut(s) 164, 365, 661, 963, 1465, 1673, 1773, 1787
AluI AGCT 8 cut(s) 164, 365, 661, 963, 1465, 1673, 1773, 1787
Alw21I GWGCWC 2 cut(s) 846, 1690
Alw26I GTCTC 1 cut(s) 1022
AlwI GGATC 5 cut(s) 544, 557, 888, 901, 1383
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 4 cut(s) 242, 442, 1218, 1533
ApeKI GCWGC 2 cut(s) 1484, 1487
ApoI RAATTY 4 cut(s) 149, 208, 1151, 1394
ArsI GACNNNNNNTTYG 2 cut(s) 925, 957
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 1427, 1534
AsuC2I CCSGG 2 cut(s) 30, 1373
AsuHPI GGTGA 5 cut(s) 22, 413, 721, 863, 1370
AsuII TTCGAA 1 cut(s) 1148
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 1 cut(s) 1427
AxyI CCTNAGG 1 cut(s) 1222
BaeGI GKGCMC 1 cut(s) 127
BalI TGGCCA 1 cut(s) 444
BamHI GGATCC 2 cut(s) 549, 893
BanI GGYRCC 2 cut(s) 373, 1255
BbrPI CACGTG 2 cut(s) 440, 1234
BbsI GAAGAC 2 cut(s) 1292, 1407
Bbv12I GWGCWC 2 cut(s) 846, 1690
BbvI GCAGC 2 cut(s) 1471, 1474
BccI CCATC 8 cut(s) 7, 533, 616, 884, 1038, 1345, 1630, 1756
BciT130I CCWGG 1 cut(s) 1725
BciVI GTATCC 1 cut(s) 491
BcnI CCSGG 2 cut(s) 30, 1373
BcoDI GTCTC 1 cut(s) 1022
BfaI CTAG 5 cut(s) 1340, 1361, 1431, 1502, 1691
BfmI CTRYAG 3 cut(s) 985, 1488, 1711
BfrI CTTAAG 1 cut(s) 803
BfuAI ACCTGC 1 cut(s) 697
BfuI GTATCC 1 cut(s) 491
BisI GCNGC 2 cut(s) 1485, 1488
BlsI GCNGC 2 cut(s) 1486, 1489
BmcAI AGTACT 2 cut(s) 1057, 1082
Bme1390I CCNGG 3 cut(s) 30, 1373, 1725
Bme18I GGWCC 1 cut(s) 1427
BmeRI GACNNNNNGTC 1 cut(s) 1587
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 2 cut(s) 1427, 1534
BmiI GGNNCC 5 cut(s) 375, 551, 895, 1121, 1257
BmrFI CCNGG 3 cut(s) 30, 1373, 1725
BmrI ACTGGG 2 cut(s) 584, 1562
BmsI GCATC 2 cut(s) 286, 693
BmuI ACTGGG 2 cut(s) 584, 1562
BpiI GAAGAC 2 cut(s) 1292, 1407
BpmI CTGGAG 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 1148
BpuEI CTTGAG 1 cut(s) 1235
BpuMI CCSGG 2 cut(s) 30, 1373
BsaAI YACGTR 2 cut(s) 440, 1234
BsaHI GRCGYC 1 cut(s) 631
BsaJI CCNNGG 4 cut(s) 80, 327, 1371, 1423
BsaWI WCCGGW 1 cut(s) 1380
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 994, 1024
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 838, 1636
Bse1I ACTGG 7 cut(s) 418, 551, 590, 718, 1536, 1568, 1588
Bse21I CCTNAGG 1 cut(s) 1222
Bse3DI GCAATG 2 cut(s) 192, 997
BseBI CCWGG 1 cut(s) 1725
BseDI CCNNGG 4 cut(s) 80, 327, 1371, 1423
BseGI GGATG 6 cut(s) 301, 608, 708, 1049, 1621, 1672
BseLI CCNNNNNNNGG 3 cut(s) 292, 838, 1636
BseMI GCAATG 2 cut(s) 192, 997
BseMII CTCAG 4 cut(s) 110, 179, 1161, 1236
BseNI ACTGG 7 cut(s) 418, 551, 590, 718, 1536, 1568, 1588
BseRI GAGGAG 2 cut(s) 133, 1475
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 2 cut(s) 1471, 1474
BshFI GGCC 4 cut(s) 244, 444, 1220, 1535
BshNI GGYRCC 2 cut(s) 373, 1255
BsiHKAI GWGCWC 2 cut(s) 846, 1690
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 29, 1373, 1381
BslFI GGGAC 6 cut(s) 129, 642, 794, 1221, 1354, 1550
BslI CCNNNNNNNGG 3 cut(s) 292, 838, 1636
BsmAI GTCTC 1 cut(s) 1022
BsmFI GGGAC 6 cut(s) 129, 642, 794, 1221, 1354, 1550
BsmI GAATGC 1 cut(s) 1434
BsnI GGCC 4 cut(s) 244, 444, 1220, 1535
BsoBI CYCGRG 1 cut(s) 423
Bsp119I TTCGAA 1 cut(s) 1148
Bsp1286I GDGCHC 3 cut(s) 127, 846, 1690
Bsp143I GATC 5 cut(s) 549, 893, 928, 1375, 1693
BspANI GGCC 4 cut(s) 244, 444, 1220, 1535
BspCNI CTCAG 4 cut(s) 111, 178, 1162, 1235
BspHI TCATGA 2 cut(s) 535, 1826
BspLI GGNNCC 5 cut(s) 375, 551, 895, 1121, 1257
BspMAI CTGCAG 1 cut(s) 1492
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 5 cut(s) 544, 557, 888, 901, 1383
BspT104I TTCGAA 1 cut(s) 1148
BspT107I GGYRCC 2 cut(s) 373, 1255
BspTI CTTAAG 1 cut(s) 803
BsrDI GCAATG 2 cut(s) 192, 997
BsrI ACTGG 7 cut(s) 418, 551, 590, 718, 1536, 1568, 1588
BssECI CCNNGG 4 cut(s) 80, 327, 1371, 1423
BssMI GATC 5 cut(s) 549, 893, 928, 1375, 1693
BssNI GRCGYC 1 cut(s) 631
BssT1I CCWWGG 3 cut(s) 80, 327, 1423
Bst2UI CCWGG 1 cut(s) 1725
Bst4CI ACNGT 5 cut(s) 451, 937, 957, 1006, 1603
BstACI GRCGYC 1 cut(s) 631
BstAFI CTTAAG 1 cut(s) 803
BstBAI YACGTR 2 cut(s) 440, 1234
BstBI TTCGAA 1 cut(s) 1148
BstC8I GCNNGC 5 cut(s) 691, 753, 1550, 1708, 1789
BstDEI CTNAG 6 cut(s) 119, 165, 872, 1170, 1222, 1282
BstF5I GGATG 6 cut(s) 301, 608, 708, 1049, 1621, 1672
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 5 cut(s) 552, 896, 931, 1378, 1696
BstMAI GTCTC 1 cut(s) 1022
BstMBI GATC 5 cut(s) 549, 893, 928, 1375, 1693
BstMWI GCNNNNNNNGC 2 cut(s) 371, 380
BstNI CCWGG 1 cut(s) 1725
BstNSI RCATGY 4 cut(s) 432, 787, 880, 1552
BstSCI CCNGG 3 cut(s) 28, 1371, 1723
BstSFI CTRYAG 3 cut(s) 985, 1488, 1711
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 1471, 1474
BstV2I GAAGAC 2 cut(s) 1292, 1407
BstX2I RGATCY 2 cut(s) 549, 893
BstXI CCANNNNNNTGG 2 cut(s) 1544, 1731
BstYI RGATCY 2 cut(s) 549, 893
Bsu36I CCTNAGG 1 cut(s) 1222
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 4 cut(s) 244, 444, 1220, 1535
BtsCI GGATG 6 cut(s) 301, 608, 708, 1049, 1621, 1672
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 5 cut(s) 691, 753, 1550, 1708, 1789
CciI TCATGA 2 cut(s) 535, 1826
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 1427, 1534
Csp6I GTAC 4 cut(s) 1056, 1081, 1358, 1795
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviQI GTAC 4 cut(s) 1056, 1081, 1358, 1795
DdeI CTNAG 6 cut(s) 119, 165, 872, 1170, 1222, 1282
DpnI GATC 5 cut(s) 551, 895, 930, 1377, 1695
DpnII GATC 5 cut(s) 549, 893, 928, 1375, 1693
DriI GACNNNNNGTC 1 cut(s) 1587
EaeI YGGCCR 1 cut(s) 442
Eam1105I GACNNNNNGTC 1 cut(s) 1587
Eco130I CCWWGG 3 cut(s) 80, 327, 1423
Eco32I GATATC 1 cut(s) 730
Eco47I GGWCC 1 cut(s) 1427
Eco72I CACGTG 2 cut(s) 440, 1234
Eco81I CCTNAGG 1 cut(s) 1222
Eco88I CYCGRG 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 1427
EcoRII CCWGG 1 cut(s) 1723
EcoRV GATATC 1 cut(s) 730
EcoT14I CCWWGG 3 cut(s) 80, 327, 1423
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 3 cut(s) 80, 327, 1423
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FaqI GGGAC 6 cut(s) 129, 642, 794, 1221, 1354, 1550
FblI GTMKAC 2 cut(s) 859, 984
Fnu4HI GCNGC 2 cut(s) 1485, 1488
FokI GGATG 6 cut(s) 308, 595, 715, 1056, 1628, 1679
Fsp4HI GCNGC 2 cut(s) 1485, 1488
FspBI CTAG 5 cut(s) 1340, 1361, 1431, 1502, 1691
GlaI GCGC 1 cut(s) 140
GluI GCNGC 2 cut(s) 1485, 1488
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 4 cut(s) 244, 444, 1220, 1535
HapII CCGG 3 cut(s) 29, 1373, 1381
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 631
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HindIII AAGCTT 2 cut(s) 961, 1671
HinfI GANTC 9 cut(s) 25, 63, 70, 218, 332, 585, 1412, 1622, 1811
HpaII CCGG 3 cut(s) 29, 1373, 1381
HphI GGTGA 5 cut(s) 22, 413, 721, 863, 1370
Hpy166II GTNNAC 4 cut(s) 860, 985, 1346, 1529
Hpy188I TCNGA 5 cut(s) 168, 223, 319, 1171, 1440
Hpy8I GTNNAC 4 cut(s) 860, 985, 1346, 1529
HpyCH4III ACNGT 5 cut(s) 451, 937, 957, 1006, 1603
HpyCH4IV ACGT 4 cut(s) 439, 509, 631, 1233
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 380
HpyF3I CTNAG 6 cut(s) 119, 165, 872, 1170, 1222, 1282
HpySE526I ACGT 4 cut(s) 439, 509, 631, 1233
Hsp92I GRCGYC 1 cut(s) 631
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 5 cut(s) 549, 893, 928, 1375, 1693
LmnI GCTCC 3 cut(s) 146, 841, 1462
Lsp1109I GCAGC 2 cut(s) 1471, 1474
LweI GCATC 2 cut(s) 286, 693
MaeI CTAG 5 cut(s) 1340, 1361, 1431, 1502, 1691
MaeII ACGT 4 cut(s) 439, 509, 631, 1233
MaeIII GTNAC 7 cut(s) 358, 409, 709, 941, 1000, 1234, 1816
MalI GATC 5 cut(s) 551, 895, 930, 1377, 1695
MboI GATC 5 cut(s) 549, 893, 928, 1375, 1693
MboII GAAGA 6 cut(s) 117, 286, 1292, 1346, 1412, 1771
MflI RGATCY 2 cut(s) 549, 893
MhlI GDGCHC 3 cut(s) 127, 846, 1690
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 7 cut(s) 149, 208, 978, 1151, 1394, 1681, 1735
MluNI TGGCCA 1 cut(s) 444
MlyI GAGTC 2 cut(s) 57, 1631
MmeI TCCRAC 2 cut(s) 275, 1037
MnlI CCTC 7 cut(s) 154, 325, 658, 908, 1165, 1231, 1453
Mox20I TGGCCA 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 444
MseI TTAA 9 cut(s) 159, 512, 624, 804, 959, 1061, 1161, 1419, 1746
MslI CAYNNNNRTG 3 cut(s) 741, 849, 1112
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 3 cut(s) 29, 1373, 1381
MspR9I CCNGG 3 cut(s) 30, 1373, 1725
Mva1269I GAATGC 1 cut(s) 1434
MvaI CCWGG 1 cut(s) 1725
MwoI GCNNNNNNNGC 2 cut(s) 371, 380
NciI CCSGG 2 cut(s) 30, 1373
NdeII GATC 5 cut(s) 549, 893, 928, 1375, 1693
NlaIV GGNNCC 5 cut(s) 375, 551, 895, 1121, 1257
NmeAIII GCCGAG 1 cut(s) 837
NmuCI GTSAC 4 cut(s) 709, 941, 1000, 1234
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 4 cut(s) 432, 787, 880, 1552
NspV TTCGAA 1 cut(s) 1148
OliI CACNNNNGTG 1 cut(s) 849
PaeI GCATGC 1 cut(s) 1552
PaeR7I CTCGAG 1 cut(s) 423
PagI TCATGA 2 cut(s) 535, 1826
PaqCI CACCTGC 1 cut(s) 697
PciI ACATGT 1 cut(s) 783
PctI GAATGC 1 cut(s) 1434
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 7 cut(s) 25, 70, 218, 332, 585, 1412, 1811
PflMI CCANNNNNTGG 2 cut(s) 838, 1636
PfoI TCCNGGA 2 cut(s) 28, 1723
PkrI GCNGC 2 cut(s) 1486, 1489
PleI GAGTC 2 cut(s) 57, 1630
PmaCI CACGTG 2 cut(s) 440, 1234
PmlI CACGTG 2 cut(s) 440, 1234
PpsI GAGTC 2 cut(s) 57, 1630
Ppu21I YACGTR 2 cut(s) 440, 1234
PpuMI RGGWCCY 1 cut(s) 1427
PscI ACATGT 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 1427
Psp6I CCWGG 1 cut(s) 1723
PspCI CACGTG 2 cut(s) 440, 1234
PspGI CCWGG 1 cut(s) 1723
PspN4I GGNNCC 5 cut(s) 375, 551, 895, 1121, 1257
PspPI GGNCC 2 cut(s) 1427, 1534
PspPPI RGGWCCY 1 cut(s) 1427
PspXI VCTCGAGB 1 cut(s) 423
PstI CTGCAG 1 cut(s) 1492
PsuI RGATCY 2 cut(s) 549, 893
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 4 cut(s) 1057, 1082, 1359, 1796
RsaNI GTAC 4 cut(s) 1056, 1081, 1358, 1795
RseI CAYNNNNRTG 3 cut(s) 741, 849, 1112
SaqAI TTAA 9 cut(s) 159, 512, 624, 804, 959, 1061, 1161, 1419, 1746
SatI GCNGC 2 cut(s) 1485, 1488
Sau3AI GATC 5 cut(s) 549, 893, 928, 1375, 1693
Sau96I GGNCC 2 cut(s) 1427, 1534
ScaI AGTACT 2 cut(s) 1057, 1082
SchI GAGTC 2 cut(s) 57, 1631
ScrFI CCNGG 3 cut(s) 30, 1373, 1725
SduI GDGCHC 3 cut(s) 127, 846, 1690
SfaNI GCATC 2 cut(s) 286, 693
SfcI CTRYAG 3 cut(s) 985, 1488, 1711
Sfr274I CTCGAG 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 1148
SinI GGWCC 1 cut(s) 1427
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 3 cut(s) 741, 849, 1112
SmlI CTYRAG 3 cut(s) 423, 803, 1250
SmoI CTYRAG 3 cut(s) 423, 803, 1250
SphI GCATGC 1 cut(s) 1552
Sse9I AATT 7 cut(s) 149, 208, 978, 1151, 1394, 1681, 1735
SspMI CTAG 5 cut(s) 1340, 1361, 1431, 1502, 1691
StyD4I CCNGG 3 cut(s) 28, 1371, 1723
StyI CCWWGG 3 cut(s) 80, 327, 1423
TaaI ACNGT 5 cut(s) 451, 937, 957, 1006, 1603
TaiI ACGT 4 cut(s) 442, 512, 634, 1236
TaqI TCGA 3 cut(s) 424, 931, 1148
TasI AATT 7 cut(s) 149, 208, 978, 1151, 1394, 1681, 1735
TatI WGTACW 4 cut(s) 1055, 1080, 1357, 1794
TfiI GAWTC 7 cut(s) 25, 70, 218, 332, 585, 1412, 1811
Tru1I TTAA 9 cut(s) 159, 512, 624, 804, 959, 1061, 1161, 1419, 1746
Tru9I TTAA 9 cut(s) 159, 512, 624, 804, 959, 1061, 1161, 1419, 1746
TseFI GTSAC 4 cut(s) 709, 941, 1000, 1234
TseI GCWGC 2 cut(s) 1484, 1487
Tsp45I GTSAC 4 cut(s) 709, 941, 1000, 1234
TspDTI ATGAA 9 cut(s) 45, 273, 344, 639, 725, 1292, 1683, 1772, 1824
Van91I CCANNNNNTGG 2 cut(s) 838, 1636
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 1427
XapI RAATTY 4 cut(s) 149, 208, 1151, 1394
XbaI TCTAGA 1 cut(s) 1690
XceI RCATGY 4 cut(s) 432, 787, 880, 1552
XhoI CTCGAG 1 cut(s) 423
XmiI GTMKAC 2 cut(s) 859, 984
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 5 cut(s) 1340, 1361, 1431, 1502, 1691
ZraI GACGTC 1 cut(s) 632
ZrmI AGTACT 2 cut(s) 1057, 1082
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.