Rroxscaffold_5G00332870

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009675
Physical Location & Seq
Reverse (-)
222917 .. 233401
10485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00332870.1

Sequence Viewer

Length: 1491 bp
ATGCACATTGTTCATGTTACAGCTGAAATGGCACCAATAGCGAAAGTTGGTGGTCTTGGTGATGTTGTAACTGGACTTGCTCGAGCATGTATATCACGTGGCCACACAGTGCATATAATGCTTCCTTTCTATGAGTGTATCCAGAAACATCAAATCAATGACTTGACGTTAATCACAACATATAACTCTTATCATGATGGAAACTGGATCCCCACTAATGCATATCATGGGGTTGTTTCAGGAATCCCAGTTATATTTCTTGAACCATCCTGTCAGTTCTTTAAGGGACATCAGGTGTATGGGGGTCCATACAATGAGCTGGAGGCATATATGTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGCAGGTGACTGGAACACAACCTGATATCATTCATGTCCATGAGTGGCAGGCTGGTGCTTTACCCATACTTTATTGGGACATGTATCATCATCTTTCACTTAAGAAGCCGAGAATAGTATTGACAATCCATAACATGGAGCACTATGGTGAATGTAGACAAGAGCAACTTAGCATGTGTGGTCTTGATGGATCCCTCTATGCAACCTTTGACAAGGCAGTTGATGATCGAACTGTTGGTCACAATCCCGAACGGTTAAGCTTATTGAAAGGTGGAATTGTCTACAGCAATGCAGTAGTCACAGTTTCTCCAACATATCTCAAAGAGACACTCTGCTCTGGATGGCTTTCAAGTACTTTGATGAGAAATCGTGATAAGTACTTTGGTATAGTAAATGGAATAGACACTACAATGTGGAACCCTTCTACTGATGTTTTCTTGCCTTCGAAATTCAATGTTAAAAACTCAGAGGGCAAGAAGTTATGCAAATACTATGTTCAAAGGGGACTTGGTTTGGCCTCAGGCGACCACGTGACAGATACTACTCTCAAGGTGCCTTTGGTTGTCTGTATTACTCGCTTAGTTGCACAAAAGGGTCTTCATTTGATTACTCATGCAATCAAGCAAGTTGAAGAATTAGGTGGACAGATGGTTGTACTGGGAAAATCCCCGGATCACCGGGTTGAAAGAGAATTTGAAGACCTTGCAGATTCGCTTAACCAAGGTCCTAGCATTCGGATTTTATTGATGTATAGTGAGGAGCTATCCCACTTACTTTATGCTGCTGCAGACATTGTTCTAGTTCCTTCCATGTATGAGCCATGTGGACTGGCCCAAATGATTGGCATGCGTTATGGGGCAGTCCCAGTAGTTAGAAAGACTGGTGGTCTTGCAGATACAGTCTTTGACATGGATGATGAGTCAAACCATGAGATGGCAAATGGGTTTGTTTTTGAAGGAATGGATGAAGCTTCCCTGAATTGTGCTCTAGATCGTGCATTTGCTTGCTATAGAAACAAGCCAGGAGAATGGAATTGCATTGTTAAGAAGGTAATGAAGATGGATAATAGCTGGAACAACACAGCTCGCAAGTACATTGAGATTTATGAATCAGTTACAGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

496

Amino Acids

55.6

Weight (kDa)

6.08

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 2 - 231 4.7e-55 Starch synthase catalytic domain
Glyco_transf_4 PF13439 16 - 178 5.7e-09 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 305 - 457 8.8e-14 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 307 - 450 1.9e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccB1I GGYRCC 2 cut(s) 31, 916
AccB7I CCANNNNNTGG 2 cut(s) 499, 1297
AccI GTMKAC 2 cut(s) 520, 645
AclWI GGATC 5 cut(s) 202, 215, 549, 562, 1044
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 2 cut(s) 812, 1055
AcvI CACGTG 2 cut(s) 98, 895
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 4 cut(s) 718, 743, 1020, 1457
AfiI CCNNNNNNNGG 2 cut(s) 499, 1297
AflII CTTAAG 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 444
AhdI GACNNNNNGTC 1 cut(s) 1248
AjnI CCWGG 1 cut(s) 1384
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 7 cut(s) 23, 319, 624, 1126, 1334, 1434, 1448
AluI AGCT 7 cut(s) 23, 319, 624, 1126, 1334, 1434, 1448
Alw21I GWGCWC 2 cut(s) 507, 1351
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 5 cut(s) 202, 215, 549, 562, 1044
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 3 cut(s) 100, 879, 1194
ApeKI GCWGC 3 cut(s) 364, 1145, 1148
ApoI RAATTY 2 cut(s) 812, 1055
ArsI GACNNNNNNTTYG 2 cut(s) 586, 618
AspS9I GGNCC 3 cut(s) 305, 1088, 1195
AsuC2I CCSGG 2 cut(s) 1034, 1043
AsuHPI GGTGA 4 cut(s) 71, 382, 524, 1031
AsuII TTCGAA 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 2 cut(s) 305, 1088
AxyI CCTNAGG 1 cut(s) 883
BalI TGGCCA 1 cut(s) 102
BamHI GGATCC 2 cut(s) 207, 554
BanI GGYRCC 2 cut(s) 31, 916
BbrPI CACGTG 2 cut(s) 98, 895
BbsI GAAGAC 2 cut(s) 953, 1068
Bbv12I GWGCWC 2 cut(s) 507, 1351
BbvI GCAGC 3 cut(s) 376, 1132, 1135
BccI CCATC 7 cut(s) 191, 274, 545, 699, 1006, 1291, 1417
BciT130I CCWGG 1 cut(s) 1386
BciVI GTATCC 1 cut(s) 149
BcnI CCSGG 2 cut(s) 1034, 1043
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 3 cut(s) 1092, 1163, 1352
BfmI CTRYAG 3 cut(s) 646, 1149, 1372
BfrI CTTAAG 1 cut(s) 464
BfuAI ACCTGC 1 cut(s) 358
BfuI GTATCC 1 cut(s) 149
BisI GCNGC 3 cut(s) 365, 1146, 1149
BlsI GCNGC 3 cut(s) 366, 1147, 1150
BmcAI AGTACT 2 cut(s) 718, 743
Bme1390I CCNGG 3 cut(s) 1034, 1043, 1386
Bme18I GGWCC 2 cut(s) 305, 1088
BmeRI GACNNNNNGTC 1 cut(s) 1248
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 3 cut(s) 305, 1088, 1195
BmiI GGNNCC 6 cut(s) 33, 209, 306, 556, 782, 918
BmrFI CCNGG 3 cut(s) 1034, 1043, 1386
BmrI ACTGGG 3 cut(s) 242, 1031, 1223
BmsI GCATC 1 cut(s) 351
BmuI ACTGGG 3 cut(s) 242, 1031, 1223
BpiI GAAGAC 2 cut(s) 953, 1068
BpmI CTGGAG 1 cut(s) 341
Bpu14I TTCGAA 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 896
BpuMI CCSGG 2 cut(s) 1034, 1043
BsaAI YACGTR 2 cut(s) 98, 895
BsaJI CCNNGG 2 cut(s) 1032, 1084
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 1297
Bse1I ACTGG 8 cut(s) 76, 209, 248, 379, 1026, 1197, 1229, 1249
Bse21I CCTNAGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 658
BseBI CCWGG 1 cut(s) 1386
BseDI CCNNGG 2 cut(s) 1032, 1084
BseGI GGATG 5 cut(s) 266, 366, 710, 1282, 1333
BseLI CCNNNNNNNGG 2 cut(s) 499, 1297
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 2 cut(s) 843, 897
BseNI ACTGG 8 cut(s) 76, 209, 248, 379, 1026, 1197, 1229, 1249
BseRI GAGGAG 1 cut(s) 1136
BseXI GCAGC 3 cut(s) 376, 1132, 1135
BshFI GGCC 3 cut(s) 102, 881, 1196
BshNI GGYRCC 2 cut(s) 31, 916
BsiHKAI GWGCWC 2 cut(s) 507, 1351
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 2 cut(s) 1034, 1042
BslFI GGGAC 4 cut(s) 300, 455, 882, 1211
BslI CCNNNNNNNGG 2 cut(s) 499, 1297
BsmAI GTCTC 1 cut(s) 683
BsmFI GGGAC 4 cut(s) 300, 455, 882, 1211
BsmI GAATGC 1 cut(s) 1095
BsnI GGCC 3 cut(s) 102, 881, 1196
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 1 cut(s) 809
Bsp1286I GDGCHC 2 cut(s) 507, 1351
Bsp143I GATC 5 cut(s) 207, 554, 589, 1036, 1354
BspANI GGCC 3 cut(s) 102, 881, 1196
BspCNI CTCAG 2 cut(s) 842, 896
BspHI TCATGA 2 cut(s) 193, 1487
BspLI GGNNCC 6 cut(s) 33, 209, 306, 556, 782, 918
BspMAI CTGCAG 1 cut(s) 1153
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 5 cut(s) 202, 215, 549, 562, 1044
BspT104I TTCGAA 1 cut(s) 809
BspT107I GGYRCC 2 cut(s) 31, 916
BspTI CTTAAG 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 658
BsrI ACTGG 8 cut(s) 76, 209, 248, 379, 1026, 1197, 1229, 1249
BssECI CCNNGG 2 cut(s) 1032, 1084
BssMI GATC 5 cut(s) 207, 554, 589, 1036, 1354
BssT1I CCWWGG 1 cut(s) 1084
Bst2UI CCWGG 1 cut(s) 1386
Bst4CI ACNGT 5 cut(s) 109, 598, 618, 667, 1264
BstAFI CTTAAG 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 118
BstBAI YACGTR 2 cut(s) 98, 895
BstBI TTCGAA 1 cut(s) 809
BstC8I GCNNGC 5 cut(s) 349, 414, 1211, 1369, 1450
BstDEI CTNAG 4 cut(s) 533, 829, 883, 943
BstF5I GGATG 5 cut(s) 266, 366, 710, 1282, 1333
BstKTI GATC 5 cut(s) 210, 557, 592, 1039, 1357
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 5 cut(s) 207, 554, 589, 1036, 1354
BstMWI GCNNNNNNNGC 3 cut(s) 29, 38, 118
BstNI CCWGG 1 cut(s) 1386
BstNSI RCATGY 4 cut(s) 90, 448, 541, 1213
BstSCI CCNGG 3 cut(s) 1032, 1041, 1384
BstSFI CTRYAG 3 cut(s) 646, 1149, 1372
BstV1I GCAGC 3 cut(s) 376, 1132, 1135
BstV2I GAAGAC 2 cut(s) 953, 1068
BstX2I RGATCY 2 cut(s) 207, 554
BstXI CCANNNNNNTGG 2 cut(s) 1205, 1392
BstYI RGATCY 2 cut(s) 207, 554
Bsu36I CCTNAGG 1 cut(s) 883
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 3 cut(s) 102, 881, 1196
BtsCI GGATG 5 cut(s) 266, 366, 710, 1282, 1333
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 5 cut(s) 349, 414, 1211, 1369, 1450
CciI TCATGA 2 cut(s) 193, 1487
Cfr13I GGNCC 3 cut(s) 305, 1088, 1195
Csp6I GTAC 4 cut(s) 717, 742, 1019, 1456
CviQI GTAC 4 cut(s) 717, 742, 1019, 1456
DdeI CTNAG 4 cut(s) 533, 829, 883, 943
DpnI GATC 5 cut(s) 209, 556, 591, 1038, 1356
DpnII GATC 5 cut(s) 207, 554, 589, 1036, 1354
DraIII CACNNNGTG 1 cut(s) 109
DriI GACNNNNNGTC 1 cut(s) 1248
EaeI YGGCCR 1 cut(s) 100
Eam1105I GACNNNNNGTC 1 cut(s) 1248
Eco130I CCWWGG 1 cut(s) 1084
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 305, 1088
Eco72I CACGTG 2 cut(s) 98, 895
Eco81I CCTNAGG 1 cut(s) 883
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 1384
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 1084
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 1084
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 4 cut(s) 300, 455, 882, 1211
FblI GTMKAC 2 cut(s) 520, 645
Fnu4HI GCNGC 3 cut(s) 365, 1146, 1149
FokI GGATG 5 cut(s) 253, 373, 717, 1289, 1340
Fsp4HI GCNGC 3 cut(s) 365, 1146, 1149
FspBI CTAG 3 cut(s) 1092, 1163, 1352
GluI GCNGC 3 cut(s) 365, 1146, 1149
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 3 cut(s) 102, 881, 1196
HapII CCGG 2 cut(s) 1034, 1042
HindIII AAGCTT 2 cut(s) 622, 1332
HinfI GANTC 4 cut(s) 243, 1073, 1283, 1472
HpaII CCGG 2 cut(s) 1034, 1042
HphI GGTGA 4 cut(s) 71, 382, 524, 1031
Hpy166II GTNNAC 4 cut(s) 521, 646, 1007, 1190
Hpy188I TCNGA 2 cut(s) 832, 1101
Hpy8I GTNNAC 4 cut(s) 521, 646, 1007, 1190
HpyAV CCTTC 5 cut(s) 795, 816, 1179, 1313, 1405
HpyCH4III ACNGT 5 cut(s) 109, 598, 618, 667, 1264
HpyCH4IV ACGT 3 cut(s) 97, 167, 894
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 38, 118
HpyF3I CTNAG 4 cut(s) 533, 829, 883, 943
HpySE526I ACGT 3 cut(s) 97, 167, 894
Kzo9I GATC 5 cut(s) 207, 554, 589, 1036, 1354
LmnI GCTCC 2 cut(s) 502, 1123
Lsp1109I GCAGC 3 cut(s) 376, 1132, 1135
LweI GCATC 1 cut(s) 351
MaeI CTAG 3 cut(s) 1092, 1163, 1352
MaeII ACGT 3 cut(s) 97, 167, 894
MaeIII GTNAC 7 cut(s) 16, 67, 370, 602, 661, 895, 1477
MalI GATC 5 cut(s) 209, 556, 591, 1038, 1356
MboI GATC 5 cut(s) 207, 554, 589, 1036, 1354
MboII GAAGA 4 cut(s) 953, 1007, 1073, 1432
MflI RGATCY 2 cut(s) 207, 554
MhlI GDGCHC 2 cut(s) 507, 1351
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 6 cut(s) 639, 812, 998, 1055, 1342, 1396
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 1 cut(s) 1292
MmeI TCCRAC 1 cut(s) 698
MnlI CCTC 5 cut(s) 316, 569, 826, 892, 1114
Mox20I TGGCCA 1 cut(s) 102
Mph1103I ATGCAT 1 cut(s) 223
MscI TGGCCA 1 cut(s) 102
MseI TTAA 7 cut(s) 170, 282, 465, 620, 822, 1080, 1407
MslI CAYNNNNRTG 3 cut(s) 402, 510, 773
Msp20I TGGCCA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 23
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 2 cut(s) 1034, 1042
MspR9I CCNGG 3 cut(s) 1034, 1043, 1386
Mva1269I GAATGC 1 cut(s) 1095
MvaI CCWGG 1 cut(s) 1386
MwoI GCNNNNNNNGC 3 cut(s) 29, 38, 118
NciI CCSGG 2 cut(s) 1034, 1043
NdeII GATC 5 cut(s) 207, 554, 589, 1036, 1354
NlaIV GGNNCC 6 cut(s) 33, 209, 306, 556, 782, 918
NmeAIII GCCGAG 1 cut(s) 498
NmuCI GTSAC 4 cut(s) 370, 602, 661, 895
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 4 cut(s) 90, 448, 541, 1213
NspV TTCGAA 1 cut(s) 809
OliI CACNNNNGTG 1 cut(s) 510
PaeI GCATGC 1 cut(s) 1213
PaeR7I CTCGAG 1 cut(s) 81
PagI TCATGA 2 cut(s) 193, 1487
PaqCI CACCTGC 1 cut(s) 358
PciI ACATGT 1 cut(s) 444
PctI GAATGC 1 cut(s) 1095
PfeI GAWTC 3 cut(s) 243, 1073, 1472
PflMI CCANNNNNTGG 2 cut(s) 499, 1297
PkrI GCNGC 3 cut(s) 366, 1147, 1150
PleI GAGTC 1 cut(s) 1291
PmaCI CACGTG 2 cut(s) 98, 895
PmlI CACGTG 2 cut(s) 98, 895
PpsI GAGTC 1 cut(s) 1291
Ppu21I YACGTR 2 cut(s) 98, 895
PpuMI RGGWCCY 1 cut(s) 1088
PscI ACATGT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 1088
Psp6I CCWGG 1 cut(s) 1384
PspCI CACGTG 2 cut(s) 98, 895
PspGI CCWGG 1 cut(s) 1384
PspN4I GGNNCC 6 cut(s) 33, 209, 306, 556, 782, 918
PspPI GGNCC 3 cut(s) 305, 1088, 1195
PspPPI RGGWCCY 1 cut(s) 1088
PspXI VCTCGAGB 1 cut(s) 81
PstI CTGCAG 1 cut(s) 1153
PsuI RGATCY 2 cut(s) 207, 554
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 4 cut(s) 718, 743, 1020, 1457
RsaNI GTAC 4 cut(s) 717, 742, 1019, 1456
RseI CAYNNNNRTG 3 cut(s) 402, 510, 773
SaqAI TTAA 7 cut(s) 170, 282, 465, 620, 822, 1080, 1407
SatI GCNGC 3 cut(s) 365, 1146, 1149
Sau3AI GATC 5 cut(s) 207, 554, 589, 1036, 1354
Sau96I GGNCC 3 cut(s) 305, 1088, 1195
ScaI AGTACT 2 cut(s) 718, 743
SchI GAGTC 1 cut(s) 1292
ScrFI CCNGG 3 cut(s) 1034, 1043, 1386
SduI GDGCHC 2 cut(s) 507, 1351
SfaNI GCATC 1 cut(s) 351
SfcI CTRYAG 3 cut(s) 646, 1149, 1372
Sfr274I CTCGAG 1 cut(s) 81
SfuI TTCGAA 1 cut(s) 809
SinI GGWCC 2 cut(s) 305, 1088
SlaI CTCGAG 1 cut(s) 81
SmiMI CAYNNNNRTG 3 cut(s) 402, 510, 773
SmlI CTYRAG 3 cut(s) 81, 464, 911
SmoI CTYRAG 3 cut(s) 81, 464, 911
SphI GCATGC 1 cut(s) 1213
Sse9I AATT 6 cut(s) 639, 812, 998, 1055, 1342, 1396
SspMI CTAG 3 cut(s) 1092, 1163, 1352
StyD4I CCNGG 3 cut(s) 1032, 1041, 1384
StyI CCWWGG 1 cut(s) 1084
TaaI ACNGT 5 cut(s) 109, 598, 618, 667, 1264
TaiI ACGT 3 cut(s) 100, 170, 897
TaqI TCGA 3 cut(s) 82, 592, 809
TasI AATT 6 cut(s) 639, 812, 998, 1055, 1342, 1396
TatI WGTACW 4 cut(s) 716, 741, 1018, 1455
TfiI GAWTC 3 cut(s) 243, 1073, 1472
Tru1I TTAA 7 cut(s) 170, 282, 465, 620, 822, 1080, 1407
Tru9I TTAA 7 cut(s) 170, 282, 465, 620, 822, 1080, 1407
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 4 cut(s) 370, 602, 661, 895
TseI GCWGC 3 cut(s) 364, 1145, 1148
Tsp45I GTSAC 4 cut(s) 370, 602, 661, 895
TspDTI ATGAA 5 cut(s) 386, 953, 1344, 1433, 1485
TspRI CASTG 1 cut(s) 114
Van91I CCANNNNNTGG 2 cut(s) 499, 1297
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 305, 1088
XapI RAATTY 2 cut(s) 812, 1055
XbaI TCTAGA 1 cut(s) 1351
XceI RCATGY 4 cut(s) 90, 448, 541, 1213
XhoI CTCGAG 1 cut(s) 81
XmiI GTMKAC 2 cut(s) 520, 645
XspI CTAG 3 cut(s) 1092, 1163, 1352
ZrmI AGTACT 2 cut(s) 718, 743
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.