pycom420g00570

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0009675
Physical Location & Seq
Reverse (-)
674005 .. 676044
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00570.6

Sequence Viewer

Length: 978 bp
ATGATCTCGATTGGGTCTCTGAAACCAAAAATGGAGACGACAGCAATTGCGATTGCGGGAGACTGGTCTTCTTGTTTTGAAACAAGAAGAAGCAGCTTATGTCATCCTCCTCCGACGAGCACCAGATGGAGGTGGCGGAGGCAGAGGCAGAAGCAGAAGCAGAAGCAGTCTTGCTCTTGTCAATTCCGCATTCCACAGATACAGGAATTCGCTTGGCCTTCTCCTGATGATGATATCCCCTTTTGGAAGAAGCCCTTCCCTTCTTGGGATGTCAACTTGGAACCTCCCTCCCAAGTAATTAAAGATTCCAAACTCATGCACCTAGTTCATGTCACCGCTGAAATGGCACCCATTGCCAAAGTTGGTGGTCTTGCTGATGTTGTCACTGGACTTGCTCGAGCTTCTTTATTGCGCGGCCACACTGTACATGTAATGCTTCCTTTCTACGAGTGTATCCAGAAGCACCAGATCAATGACCTCACGTTAGTCGCAACATATAACTCTTTTCACCATGGAAACTGGATTCCCACTAATGCATACACTGGGATAGTTTCAGGAATCCCAGTTATACTTATTGACCCATCACATCACTTCTTTAAGGGCCATCATGTCTATGGGGGTTCCTACAATGAGCTCGAAGCATATCTCTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGGCTACTGGAACACAACCTGATATTATTCATGTCCATGAATGGCAGACAGGTGCTTTGCCGTTACTTTATTGGGATATGTATCATTATCTCTCACTTAAGAAACCGAGAGTAGTATTGACAATCCATAACATGGAGCACTATGGTGAATGTAGACAAGAGCAACTAAGCATGTGCGGTCTTGATGGATCTTTGTATGCTAACTTTGAAAAAGCAATTGATGATCGAACTGTTGGCCATAATCCTGAGAGGTTAAGCTTATTGAAAGGTGGAATTGTCTACAGCAATGCAGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

326

Amino Acids

37.08

Weight (kDa)

8.09

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccB7I CCANNNNNTGG 1 cut(s) 811
AccI GTMKAC 2 cut(s) 832, 957
AccII CGCG 1 cut(s) 414
AciI CCGC 6 cut(s) 56, 136, 187, 336, 414, 855
AclWI GGATC 1 cut(s) 874
AcoI YGGCCR 2 cut(s) 415, 913
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 1 cut(s) 426
AfiI CCNNNNNNNGG 3 cut(s) 129, 265, 811
AflII CTTAAG 1 cut(s) 776
AflIII ACRYGT 1 cut(s) 427
AgsI TTSAA 4 cut(s) 80, 671, 887, 943
AleI CACNNNNGTG 1 cut(s) 822
AluBI AGCT 4 cut(s) 96, 401, 634, 936
AluI AGCT 4 cut(s) 96, 401, 634, 936
Alw21I GWGCWC 3 cut(s) 122, 636, 819
Alw26I GTCTC 3 cut(s) 21, 29, 54
AlwI GGATC 1 cut(s) 874
Ama87I CYCGRG 1 cut(s) 396
AoxI GGCC 4 cut(s) 215, 415, 601, 913
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 206
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 1 cut(s) 601
AsuHPI GGTGA 3 cut(s) 325, 500, 836
AvaI CYCGRG 1 cut(s) 396
BaeI ACNNNNGTAYC 2 cut(s) 436, 469
BalI TGGCCA 1 cut(s) 915
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 636
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 3 cut(s) 122, 636, 819
BbvI GCAGC 1 cut(s) 105
BccI CCATC 4 cut(s) 120, 589, 612, 857
BceAI ACGGC 1 cut(s) 724
BciVI GTATCC 1 cut(s) 464
BcoDI GTCTC 3 cut(s) 21, 29, 54
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 958
BfrI CTTAAG 1 cut(s) 776
BfuI GTATCC 1 cut(s) 464
BisI GCNGC 2 cut(s) 94, 415
BlsI GCNGC 2 cut(s) 95, 416
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 3 cut(s) 282, 348, 622
BmrI ACTGGG 2 cut(s) 552, 557
BmsI GCATC 1 cut(s) 666
BmuI ACTGGG 2 cut(s) 552, 557
BpiI GAAGAC 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 759
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 511
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 265, 811
Bse1I ACTGG 6 cut(s) 68, 391, 524, 547, 563, 691
Bse3DI GCAATG 2 cut(s) 351, 970
Bse8I GATNNNNATC 1 cut(s) 759
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 3 cut(s) 103, 274, 681
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 3 cut(s) 129, 265, 811
BseMI GCAATG 2 cut(s) 351, 970
BseMII CTCAG 1 cut(s) 915
BseNI ACTGG 6 cut(s) 68, 391, 524, 547, 563, 691
BseRI GAGGAG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 4 cut(s) 217, 417, 603, 915
BshNI GGYRCC 1 cut(s) 346
BsiHKAI GWGCWC 3 cut(s) 122, 636, 819
BsiHKCI CYCGRG 1 cut(s) 396
BslI CCNNNNNNNGG 3 cut(s) 129, 265, 811
BsmAI GTCTC 3 cut(s) 21, 29, 54
BsmBI CGTCTC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 4 cut(s) 217, 417, 603, 915
Bso31I GGTCTC 1 cut(s) 21
BsoBI CYCGRG 1 cut(s) 396
Bsp1286I GDGCHC 3 cut(s) 122, 636, 819
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 4 cut(s) 3, 468, 866, 901
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 6 cut(s) 56, 136, 187, 336, 414, 855
BspANI GGCC 4 cut(s) 217, 417, 603, 915
BspCNI CTCAG 1 cut(s) 916
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 3 cut(s) 282, 348, 622
BspPI GGATC 1 cut(s) 874
BspT107I GGYRCC 1 cut(s) 346
BspTI CTTAAG 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 21
BsrDI GCAATG 2 cut(s) 351, 970
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 6 cut(s) 68, 391, 524, 547, 563, 691
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 4 cut(s) 3, 468, 866, 901
BssT1I CCWWGG 1 cut(s) 511
Bst4CI ACNGT 2 cut(s) 424, 910
Bst6I CTCTTC 1 cut(s) 653
BstAFI CTTAAG 1 cut(s) 776
BstAPI GCANNNNNTGC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 2 cut(s) 664, 681
BstDEI CTNAG 2 cut(s) 845, 924
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 3 cut(s) 103, 274, 681
BstFNI CGCG 1 cut(s) 414
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 4 cut(s) 6, 471, 869, 904
BstMAI GTCTC 3 cut(s) 21, 29, 54
BstMBI GATC 4 cut(s) 3, 468, 866, 901
BstMWI GCNNNNNNNGC 2 cut(s) 344, 353
BstNSI RCATGY 2 cut(s) 431, 853
BstSFI CTRYAG 1 cut(s) 958
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 866
BstYI RGATCY 1 cut(s) 866
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 4 cut(s) 217, 417, 603, 915
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 3 cut(s) 103, 274, 681
BtsIMutI CAGTG 3 cut(s) 384, 420, 540
Cac8I GCNNGC 2 cut(s) 664, 681
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 1 cut(s) 601
Csp6I GTAC 1 cut(s) 425
CspCI CAANNNNNGTGG 2 cut(s) 346, 381
CviAII CATG 9 cut(s) 316, 329, 428, 512, 608, 710, 716, 811, 850
CviQI GTAC 1 cut(s) 425
DdeI CTNAG 2 cut(s) 845, 924
DpnI GATC 4 cut(s) 5, 470, 868, 903
DpnII GATC 4 cut(s) 3, 468, 866, 901
EaeI YGGCCR 2 cut(s) 415, 913
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 634
Eco88I CYCGRG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 206
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 511
EcoT22I ATGCAT 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 9 cut(s) 319, 332, 431, 515, 611, 713, 719, 814, 853
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FatI CATG 9 cut(s) 315, 328, 427, 511, 607, 709, 715, 810, 849
FauI CCCGC 1 cut(s) 49
FblI GTMKAC 2 cut(s) 832, 957
Fnu4HI GCNGC 2 cut(s) 94, 415
FokI GGATG 3 cut(s) 90, 281, 688
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 2 cut(s) 94, 415
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 413
GluI GCNGC 2 cut(s) 94, 415
HaeIII GGCC 4 cut(s) 217, 417, 603, 915
HhaI GCGC 1 cut(s) 414
Hin1II CATG 9 cut(s) 319, 332, 431, 515, 611, 713, 719, 814, 853
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 934
HinfI GANTC 3 cut(s) 305, 523, 558
HphI GGTGA 3 cut(s) 325, 500, 836
Hpy166II GTNNAC 3 cut(s) 274, 833, 958
Hpy188I TCNGA 2 cut(s) 21, 114
Hpy188III TCNNGA 6 cut(s) 7, 224, 457, 555, 860, 923
Hpy8I GTNNAC 3 cut(s) 274, 833, 958
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 3 cut(s) 228, 265, 270
HpyCH4III ACNGT 2 cut(s) 424, 910
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 4 cut(s) 319, 536, 679, 968
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 353
HpyF3I CTNAG 2 cut(s) 845, 924
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 9 cut(s) 319, 332, 431, 515, 611, 713, 719, 814, 853
HspAI GCGC 1 cut(s) 412
Kzo9I GATC 4 cut(s) 3, 468, 866, 901
LmnI GCTCC 1 cut(s) 814
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 1 cut(s) 666
MaeI CTAG 1 cut(s) 323
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 331, 382, 741
MalI GATC 4 cut(s) 5, 470, 868, 903
MboI GATC 4 cut(s) 3, 468, 866, 901
MboII GAAGA 4 cut(s) 60, 99, 259, 640
MfeI CAATTG 2 cut(s) 45, 894
MflI RGATCY 1 cut(s) 866
MhlI GDGCHC 3 cut(s) 122, 636, 819
MlsI TGGCCA 1 cut(s) 915
MluCI AATT 6 cut(s) 45, 182, 206, 297, 894, 951
MluNI TGGCCA 1 cut(s) 915
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 9 cut(s) 117, 120, 123, 132, 138, 294, 298, 488, 921
Mox20I TGGCCA 1 cut(s) 915
Mph1103I ATGCAT 1 cut(s) 538
MroXI GAANNNNTTC 1 cut(s) 254
MscI TGGCCA 1 cut(s) 915
MseI TTAA 4 cut(s) 300, 597, 777, 932
MslI CAYNNNNRTG 3 cut(s) 612, 714, 822
Msp20I TGGCCA 1 cut(s) 915
MspA1I CMGCKG 1 cut(s) 338
MspCI CTTAAG 1 cut(s) 776
MunI CAATTG 2 cut(s) 45, 894
Mva1269I GAATGC 1 cut(s) 189
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 344, 353
NcoI CCATGG 1 cut(s) 511
NdeII GATC 4 cut(s) 3, 468, 866, 901
NlaIII CATG 9 cut(s) 319, 332, 431, 515, 611, 713, 719, 814, 853
NlaIV GGNNCC 3 cut(s) 282, 348, 622
NmuCI GTSAC 2 cut(s) 331, 382
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 2 cut(s) 431, 853
OliI CACNNNNGTG 1 cut(s) 822
PaeR7I CTCGAG 1 cut(s) 396
PciI ACATGT 1 cut(s) 427
PctI GAATGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 3 cut(s) 305, 523, 558
PflMI CCANNNNNTGG 1 cut(s) 811
PkrI GCNGC 2 cut(s) 95, 416
PscI ACATGT 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 636
PspN4I GGNNCC 3 cut(s) 282, 348, 622
PspPI GGNCC 1 cut(s) 601
PspXI VCTCGAGB 1 cut(s) 396
PsuI RGATCY 1 cut(s) 866
RsaI GTAC 1 cut(s) 426
RsaNI GTAC 1 cut(s) 425
RseI CAYNNNNRTG 3 cut(s) 612, 714, 822
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 4 cut(s) 300, 597, 777, 932
SatI GCNGC 2 cut(s) 94, 415
Sau3AI GATC 4 cut(s) 3, 468, 866, 901
Sau96I GGNCC 1 cut(s) 601
SduI GDGCHC 3 cut(s) 122, 636, 819
SfaNI GCATC 1 cut(s) 666
SfcI CTRYAG 1 cut(s) 958
Sfr274I CTCGAG 1 cut(s) 396
SlaI CTCGAG 1 cut(s) 396
SmiMI CAYNNNNRTG 3 cut(s) 612, 714, 822
SmlI CTYRAG 2 cut(s) 396, 776
SmoI CTYRAG 2 cut(s) 396, 776
Sse9I AATT 6 cut(s) 45, 182, 206, 297, 894, 951
SsiI CCGC 6 cut(s) 56, 136, 187, 336, 414, 855
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 636
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 2 cut(s) 424, 910
TaiI ACGT 1 cut(s) 485
TaqI TCGA 4 cut(s) 8, 397, 636, 904
TasI AATT 6 cut(s) 45, 182, 206, 297, 894, 951
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 3 cut(s) 305, 523, 558
Tru1I TTAA 4 cut(s) 300, 597, 777, 932
Tru9I TTAA 4 cut(s) 300, 597, 777, 932
TscAI CASTG 3 cut(s) 391, 427, 547
TseFI GTSAC 2 cut(s) 331, 382
TseI GCWGC 1 cut(s) 93
Tsp45I GTSAC 2 cut(s) 331, 382
TspDTI ATGAA 3 cut(s) 317, 698, 732
TspRI CASTG 3 cut(s) 391, 427, 547
Van91I CCANNNNNTGG 1 cut(s) 811
Vha464I CTTAAG 1 cut(s) 776
XapI RAATTY 1 cut(s) 206
XceI RCATGY 2 cut(s) 431, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XhoI CTCGAG 1 cut(s) 396
XmiI GTMKAC 2 cut(s) 832, 957
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.