Rw0G012850

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00569
OG0009675
Physical Location & Seq
Reverse (-)
22440 .. 31864
9425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012850.1

Sequence Viewer

Length: 1602 bp
GTCGGCATTGCAAAACCAACAAATTTGACTGATTCTGATATTCAGGGATTTGTTTGGCCTTCTCCAACAGATGAAATCCCATTTTGGAAGAAGGAGTTCCCTTCTTGGGATGCAAACTTGGAACTTCCCTCTGAACAAACCAAGGATTCCAAACTCATGCACATTGTTCATGTTACAGCTGAAATGGCACCAATAGCAAAAGTTGGTGGTCTTGGTGATGTTGTAACTGGACTTGCTCGAGCATGTTTATCACGTGGCCACACAGTCCATATAATGCTTCCTTTCTATGAGTGTATCCAGAAACATCAAATCAATGACTTGACGTTAATCACAACATATAACTCTTATCATGATGGAAACTGGATCCCCACTAATGCATATCATGGGGTAGTTTCAGGAATCCCAGTTATATTTCTTGAACCATCCTGTCAGTTCTTTAAGGGACGTCAGGTGTATGGGGGTTCATACAATGAGCTGGAGGCATATATGTTCTTTAGTCGTGCTTGCCTTGAATGGATGCAGTGGCAGGCTGGTGCTTTACCCATACTTTATTGGGACATGTATCATCATCTTTCACTTAAGAAGCCGAGAATAGTATTGACAATCCATAACATGGAGCACTATGGTGAATGCAGACAAGAGCAACTTAGCATGTGTGGTCTTGATGGATCCCTCTATGCAACCTTTGACAAGGCAGTTGATGATCGAACTGTTGGTCACAATCCCGAACGGTTAAGCTTATTGAAAGGTGGAATTGTCTACAGCAATGCAGTAGTCACAGTTTCTCCAACATATCTCAAAGAGACACTCTGCTCTGGATGGCTTTCAAGTACTTTAATGAGAAATCGTGATAAGTACTTTGGTATAGTAAATGGAATAGACACTACGATGTGGAACCCTACTGATGTTTTCTTGCCTTCGAAATTCAATGTTAAAAACTCTGAGGGCAAGAAGTTATGCAAATACTATGTTCAAAGGGGACTTGGTTTGGCCTCAGGCAACCACGTGACAGATACTACTCTCAAGGTGCCTTTGGTTGTCTGTATTACTCGCTTAGTTGCACAAAAGGGTCTTCATTTGATTACTCATGCAATCAAGCAAGTTGAAGAACTAGGTGGACAGATGGTTGTACTAGGAAAGTCCCCGGATCACCGGATTGAAAGAGAATTTGAAGACCTTGCAGATTCGCTTAACCAAGGTCCTAGCATTCGGATTTTATTGATGTATAGTGAGGAGCTATCCCACTTACTTTATGCTGCTGCAGACATTGTTCTAGTTCCTTCCATGTATGAGCCATGTGGACTGGCCCAAATGATTGGCATGCGTTATGGGGCAGTCCCAGTAGTTAGAAAGACTGGTGGTCTTGCAGATACAGTCTTTGACATGGATGATGAGTCAAACCATCAGATGGCAAATGGGTTTGTTTTTGAAGGAATGGATGAAGCTTCCCTGAATTGTGCTCTAGATCGTGCATTTGCTTGCTATAGAAACAATCCAGGAGAATGGAATTGCATTGTTAAGAAGGTAATGAAGATGGATAATAGCTGGAACAACACAGCTCGCAAGTACATTGAGATTTATGAATCAGTTACAGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

533

Amino Acids

59.85

Weight (kDa)

5.99

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 54 - 269 2.8e-42 Starch synthase catalytic domain
Glycos_transf_1 PF00534 342 - 493 2.6e-13 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 344 - 486 2.2e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 448
AccB1I GGYRCC 2 cut(s) 187, 1027
AccB7I CCANNNNNTGG 2 cut(s) 613, 1408
AccI GTMKAC 1 cut(s) 759
AclWI GGATC 5 cut(s) 358, 371, 663, 676, 1155
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 3 cut(s) 22, 923, 1166
AcvI CACGTG 2 cut(s) 254, 1006
AcyI GRCGYC 1 cut(s) 445
AfaI GTAC 4 cut(s) 832, 857, 1131, 1568
AfiI CCNNNNNNNGG 3 cut(s) 106, 613, 1408
AflII CTTAAG 1 cut(s) 578
AflIII ACRYGT 1 cut(s) 558
AhdI GACNNNNNGTC 1 cut(s) 1359
AjnI CCWGG 1 cut(s) 1495
AleI CACNNNNGTG 1 cut(s) 624
AluBI AGCT 7 cut(s) 179, 475, 738, 1237, 1445, 1545, 1559
AluI AGCT 7 cut(s) 179, 475, 738, 1237, 1445, 1545, 1559
Alw21I GWGCWC 2 cut(s) 621, 1462
Alw26I GTCTC 1 cut(s) 797
AlwI GGATC 5 cut(s) 358, 371, 663, 676, 1155
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 4 cut(s) 56, 256, 990, 1305
ApeKI GCWGC 2 cut(s) 1256, 1259
ApoI RAATTY 3 cut(s) 22, 923, 1166
ArsI GACNNNNNNTTYG 2 cut(s) 700, 732
Asp700I GAANNNNTTC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 1199, 1306
AsuC2I CCSGG 1 cut(s) 1145
AsuHPI GGTGA 3 cut(s) 227, 638, 1142
AsuII TTCGAA 1 cut(s) 920
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 1199
AxyI CCTNAGG 1 cut(s) 994
BalI TGGCCA 1 cut(s) 258
BamHI GGATCC 2 cut(s) 363, 668
BanI GGYRCC 2 cut(s) 187, 1027
BbrPI CACGTG 2 cut(s) 254, 1006
BbsI GAAGAC 2 cut(s) 1064, 1179
Bbv12I GWGCWC 2 cut(s) 621, 1462
BbvI GCAGC 2 cut(s) 1243, 1246
BccI CCATC 8 cut(s) 347, 430, 659, 813, 1117, 1402, 1410, 1528
BciT130I CCWGG 1 cut(s) 1497
BciVI GTATCC 1 cut(s) 305
BcnI CCSGG 1 cut(s) 1145
BcoDI GTCTC 1 cut(s) 797
BfaI CTAG 5 cut(s) 1112, 1133, 1203, 1274, 1463
BfmI CTRYAG 3 cut(s) 760, 1260, 1483
BfrI CTTAAG 1 cut(s) 578
BfuI GTATCC 1 cut(s) 305
BisI GCNGC 2 cut(s) 1257, 1260
BlsI GCNGC 2 cut(s) 1258, 1261
BmcAI AGTACT 2 cut(s) 832, 857
Bme1390I CCNGG 2 cut(s) 1145, 1497
Bme18I GGWCC 1 cut(s) 1199
BmeRI GACNNNNNGTC 1 cut(s) 1359
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 2 cut(s) 1199, 1306
BmiI GGNNCC 5 cut(s) 189, 365, 670, 896, 1029
BmrFI CCNGG 2 cut(s) 1145, 1497
BmrI ACTGGG 2 cut(s) 398, 1334
BmsI GCATC 2 cut(s) 100, 507
BmuI ACTGGG 2 cut(s) 398, 1334
BpiI GAAGAC 2 cut(s) 1064, 1179
BpmI CTGGAG 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 920
BpuEI CTTGAG 1 cut(s) 1007
BpuMI CCSGG 1 cut(s) 1145
BsaAI YACGTR 2 cut(s) 254, 1006
BsaHI GRCGYC 1 cut(s) 445
BsaJI CCNNGG 3 cut(s) 141, 1143, 1195
BsaWI WCCGGW 1 cut(s) 1152
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 613, 1408
Bse1I ACTGG 6 cut(s) 232, 365, 404, 1308, 1340, 1360
Bse21I CCTNAGG 1 cut(s) 994
Bse3DI GCAATG 2 cut(s) 6, 772
BseBI CCWGG 1 cut(s) 1497
BseDI CCNNGG 3 cut(s) 141, 1143, 1195
BseGI GGATG 6 cut(s) 115, 422, 522, 824, 1393, 1444
BseLI CCNNNNNNNGG 3 cut(s) 106, 613, 1408
BseMI GCAATG 2 cut(s) 6, 772
BseMII CTCAG 2 cut(s) 933, 1008
BseNI ACTGG 6 cut(s) 232, 365, 404, 1308, 1340, 1360
BseRI GAGGAG 1 cut(s) 1247
BseXI GCAGC 2 cut(s) 1243, 1246
BshFI GGCC 4 cut(s) 58, 258, 992, 1307
BshNI GGYRCC 2 cut(s) 187, 1027
BsiHKAI GWGCWC 2 cut(s) 621, 1462
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 2 cut(s) 1145, 1153
BslFI GGGAC 5 cut(s) 456, 569, 993, 1126, 1322
BslI CCNNNNNNNGG 3 cut(s) 106, 613, 1408
BsmAI GTCTC 1 cut(s) 797
BsmFI GGGAC 5 cut(s) 456, 569, 993, 1126, 1322
BsmI GAATGC 2 cut(s) 635, 1206
BsnI GGCC 4 cut(s) 58, 258, 992, 1307
BsoBI CYCGRG 1 cut(s) 237
Bsp119I TTCGAA 1 cut(s) 920
Bsp1286I GDGCHC 2 cut(s) 621, 1462
Bsp143I GATC 5 cut(s) 363, 668, 703, 1147, 1465
BspANI GGCC 4 cut(s) 58, 258, 992, 1307
BspCNI CTCAG 2 cut(s) 934, 1007
BspHI TCATGA 2 cut(s) 349, 1598
BspLI GGNNCC 5 cut(s) 189, 365, 670, 896, 1029
BspMAI CTGCAG 1 cut(s) 1264
BspPI GGATC 5 cut(s) 358, 371, 663, 676, 1155
BspT104I TTCGAA 1 cut(s) 920
BspT107I GGYRCC 2 cut(s) 187, 1027
BspTI CTTAAG 1 cut(s) 578
BsrDI GCAATG 2 cut(s) 6, 772
BsrI ACTGG 6 cut(s) 232, 365, 404, 1308, 1340, 1360
BssECI CCNNGG 3 cut(s) 141, 1143, 1195
BssMI GATC 5 cut(s) 363, 668, 703, 1147, 1465
BssNI GRCGYC 1 cut(s) 445
BssT1I CCWWGG 2 cut(s) 141, 1195
Bst2UI CCWGG 1 cut(s) 1497
Bst4CI ACNGT 5 cut(s) 265, 712, 732, 781, 1375
BstACI GRCGYC 1 cut(s) 445
BstAFI CTTAAG 1 cut(s) 578
BstBAI YACGTR 2 cut(s) 254, 1006
BstBI TTCGAA 1 cut(s) 920
BstC8I GCNNGC 5 cut(s) 505, 528, 1322, 1480, 1561
BstDEI CTNAG 4 cut(s) 647, 942, 994, 1054
BstF5I GGATG 6 cut(s) 115, 422, 522, 824, 1393, 1444
BstKTI GATC 5 cut(s) 366, 671, 706, 1150, 1468
BstMAI GTCTC 1 cut(s) 797
BstMBI GATC 5 cut(s) 363, 668, 703, 1147, 1465
BstMWI GCNNNNNNNGC 2 cut(s) 185, 194
BstNI CCWGG 1 cut(s) 1497
BstNSI RCATGY 4 cut(s) 246, 562, 655, 1324
BstSCI CCNGG 2 cut(s) 1143, 1495
BstSFI CTRYAG 3 cut(s) 760, 1260, 1483
BstV1I GCAGC 2 cut(s) 1243, 1246
BstV2I GAAGAC 2 cut(s) 1064, 1179
BstX2I RGATCY 2 cut(s) 363, 668
BstXI CCANNNNNNTGG 2 cut(s) 1316, 1503
BstYI RGATCY 2 cut(s) 363, 668
Bsu36I CCTNAGG 1 cut(s) 994
BsuI GTATCC 1 cut(s) 305
BsuRI GGCC 4 cut(s) 58, 258, 992, 1307
BtsCI GGATG 6 cut(s) 115, 422, 522, 824, 1393, 1444
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 5 cut(s) 505, 528, 1322, 1480, 1561
CciI TCATGA 2 cut(s) 349, 1598
Cfr13I GGNCC 2 cut(s) 1199, 1306
Csp6I GTAC 4 cut(s) 831, 856, 1130, 1567
CviQI GTAC 4 cut(s) 831, 856, 1130, 1567
DdeI CTNAG 4 cut(s) 647, 942, 994, 1054
DpnI GATC 5 cut(s) 365, 670, 705, 1149, 1467
DpnII GATC 5 cut(s) 363, 668, 703, 1147, 1465
DriI GACNNNNNGTC 1 cut(s) 1359
EaeI YGGCCR 1 cut(s) 256
Eam1105I GACNNNNNGTC 1 cut(s) 1359
Eco130I CCWWGG 2 cut(s) 141, 1195
Eco47I GGWCC 1 cut(s) 1199
Eco72I CACGTG 2 cut(s) 254, 1006
Eco81I CCTNAGG 1 cut(s) 994
Eco88I CYCGRG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 1199
EcoRII CCWGG 1 cut(s) 1495
EcoT14I CCWWGG 2 cut(s) 141, 1195
EcoT22I ATGCAT 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 141, 1195
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 5 cut(s) 456, 569, 993, 1126, 1322
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 2 cut(s) 1257, 1260
FokI GGATG 6 cut(s) 122, 409, 529, 831, 1400, 1451
Fsp4HI GCNGC 2 cut(s) 1257, 1260
FspBI CTAG 5 cut(s) 1112, 1133, 1203, 1274, 1463
GluI GCNGC 2 cut(s) 1257, 1260
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 4 cut(s) 58, 258, 992, 1307
HapII CCGG 2 cut(s) 1145, 1153
Hin1I GRCGYC 1 cut(s) 445
HindIII AAGCTT 2 cut(s) 736, 1443
HinfI GANTC 6 cut(s) 32, 146, 399, 1184, 1394, 1583
HpaII CCGG 2 cut(s) 1145, 1153
HphI GGTGA 3 cut(s) 227, 638, 1142
Hpy166II GTNNAC 3 cut(s) 760, 1118, 1301
Hpy188I TCNGA 5 cut(s) 37, 133, 943, 1212, 1407
Hpy8I GTNNAC 3 cut(s) 760, 1118, 1301
HpyAV CCTTC 7 cut(s) 69, 85, 111, 927, 1290, 1424, 1516
HpyCH4III ACNGT 5 cut(s) 265, 712, 732, 781, 1375
HpyCH4IV ACGT 4 cut(s) 253, 323, 445, 1005
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 194
HpyF3I CTNAG 4 cut(s) 647, 942, 994, 1054
HpySE526I ACGT 4 cut(s) 253, 323, 445, 1005
Hsp92I GRCGYC 1 cut(s) 445
Kzo9I GATC 5 cut(s) 363, 668, 703, 1147, 1465
LmnI GCTCC 2 cut(s) 616, 1234
Lsp1109I GCAGC 2 cut(s) 1243, 1246
LweI GCATC 2 cut(s) 100, 507
MaeI CTAG 5 cut(s) 1112, 1133, 1203, 1274, 1463
MaeII ACGT 4 cut(s) 253, 323, 445, 1005
MaeIII GTNAC 6 cut(s) 172, 223, 716, 775, 1006, 1588
MalI GATC 5 cut(s) 365, 670, 705, 1149, 1467
MboI GATC 5 cut(s) 363, 668, 703, 1147, 1465
MboII GAAGA 5 cut(s) 100, 1064, 1118, 1184, 1543
MflI RGATCY 2 cut(s) 363, 668
MhlI GDGCHC 2 cut(s) 621, 1462
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 6 cut(s) 22, 753, 923, 1166, 1453, 1507
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 1 cut(s) 1403
MmeI TCCRAC 2 cut(s) 89, 812
MnlI CCTC 6 cut(s) 139, 472, 683, 937, 1003, 1225
Mox20I TGGCCA 1 cut(s) 258
Mph1103I ATGCAT 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 258
MseI TTAA 8 cut(s) 326, 438, 579, 734, 836, 933, 1191, 1518
MslI CAYNNNNRTG 2 cut(s) 624, 887
Msp20I TGGCCA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 179
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 2 cut(s) 1145, 1153
MspR9I CCNGG 2 cut(s) 1145, 1497
Mva1269I GAATGC 2 cut(s) 635, 1206
MvaI CCWGG 1 cut(s) 1497
MwoI GCNNNNNNNGC 2 cut(s) 185, 194
NciI CCSGG 1 cut(s) 1145
NdeII GATC 5 cut(s) 363, 668, 703, 1147, 1465
NlaIV GGNNCC 5 cut(s) 189, 365, 670, 896, 1029
NmeAIII GCCGAG 1 cut(s) 612
NmuCI GTSAC 3 cut(s) 716, 775, 1006
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 4 cut(s) 246, 562, 655, 1324
NspV TTCGAA 1 cut(s) 920
OliI CACNNNNGTG 1 cut(s) 624
PaeI GCATGC 1 cut(s) 1324
PaeR7I CTCGAG 1 cut(s) 237
PagI TCATGA 2 cut(s) 349, 1598
PciI ACATGT 1 cut(s) 558
PctI GAATGC 2 cut(s) 635, 1206
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 5 cut(s) 32, 146, 399, 1184, 1583
PflMI CCANNNNNTGG 2 cut(s) 613, 1408
PfoI TCCNGGA 1 cut(s) 1495
PkrI GCNGC 2 cut(s) 1258, 1261
PleI GAGTC 1 cut(s) 1402
PmaCI CACGTG 2 cut(s) 254, 1006
PmlI CACGTG 2 cut(s) 254, 1006
PpsI GAGTC 1 cut(s) 1402
Ppu21I YACGTR 2 cut(s) 254, 1006
PpuMI RGGWCCY 1 cut(s) 1199
PscI ACATGT 1 cut(s) 558
Psp5II RGGWCCY 1 cut(s) 1199
Psp6I CCWGG 1 cut(s) 1495
PspCI CACGTG 2 cut(s) 254, 1006
PspGI CCWGG 1 cut(s) 1495
PspN4I GGNNCC 5 cut(s) 189, 365, 670, 896, 1029
PspPI GGNCC 2 cut(s) 1199, 1306
PspPPI RGGWCCY 1 cut(s) 1199
PspXI VCTCGAGB 1 cut(s) 237
PstI CTGCAG 1 cut(s) 1264
PsuI RGATCY 2 cut(s) 363, 668
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 4 cut(s) 832, 857, 1131, 1568
RsaNI GTAC 4 cut(s) 831, 856, 1130, 1567
RseI CAYNNNNRTG 2 cut(s) 624, 887
SaqAI TTAA 8 cut(s) 326, 438, 579, 734, 836, 933, 1191, 1518
SatI GCNGC 2 cut(s) 1257, 1260
Sau3AI GATC 5 cut(s) 363, 668, 703, 1147, 1465
Sau96I GGNCC 2 cut(s) 1199, 1306
ScaI AGTACT 2 cut(s) 832, 857
SchI GAGTC 1 cut(s) 1403
ScrFI CCNGG 2 cut(s) 1145, 1497
SduI GDGCHC 2 cut(s) 621, 1462
SfaNI GCATC 2 cut(s) 100, 507
SfcI CTRYAG 3 cut(s) 760, 1260, 1483
Sfr274I CTCGAG 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 920
SinI GGWCC 1 cut(s) 1199
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 624, 887
SmlI CTYRAG 3 cut(s) 237, 578, 1022
SmoI CTYRAG 3 cut(s) 237, 578, 1022
SphI GCATGC 1 cut(s) 1324
Sse9I AATT 6 cut(s) 22, 753, 923, 1166, 1453, 1507
SspMI CTAG 5 cut(s) 1112, 1133, 1203, 1274, 1463
StyD4I CCNGG 2 cut(s) 1143, 1495
StyI CCWWGG 2 cut(s) 141, 1195
TaaI ACNGT 5 cut(s) 265, 712, 732, 781, 1375
TaiI ACGT 4 cut(s) 256, 326, 448, 1008
TaqI TCGA 3 cut(s) 238, 706, 920
TasI AATT 6 cut(s) 22, 753, 923, 1166, 1453, 1507
TatI WGTACW 4 cut(s) 830, 855, 1129, 1566
TfiI GAWTC 5 cut(s) 32, 146, 399, 1184, 1583
Tru1I TTAA 8 cut(s) 326, 438, 579, 734, 836, 933, 1191, 1518
Tru9I TTAA 8 cut(s) 326, 438, 579, 734, 836, 933, 1191, 1518
TscAI CASTG 1 cut(s) 527
TseFI GTSAC 3 cut(s) 716, 775, 1006
TseI GCWGC 2 cut(s) 1256, 1259
Tsp45I GTSAC 3 cut(s) 716, 775, 1006
TspDTI ATGAA 7 cut(s) 87, 158, 453, 1064, 1455, 1544, 1596
TspRI CASTG 1 cut(s) 527
Van91I CCANNNNNTGG 2 cut(s) 613, 1408
Vha464I CTTAAG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 1199
XapI RAATTY 3 cut(s) 22, 923, 1166
XbaI TCTAGA 1 cut(s) 1462
XceI RCATGY 4 cut(s) 246, 562, 655, 1324
XhoI CTCGAG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 759
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 5 cut(s) 1112, 1133, 1203, 1274, 1463
ZraI GACGTC 1 cut(s) 446
ZrmI AGTACT 2 cut(s) 832, 857
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.