Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006075

Physical Location & Seq

Genomic Coordinates Forward (+)

470721 .. 476223

Gene Structure 5503 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1004100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 927 bp

ATGAACGACCTGCTAACGGATTCTTTTGAGATCCCTCGGGGTCAAGCTTCGAGAGATCAGGATATTGAACTAGGAGCACATGCTTCAATGAATTCTGGAGAACTTGGTTTGGAGAATTTCTTCAAAAAGGTTCAAGAGATCGAGAAGCAAAATGAGAAGCTTGATAAGCTTTTGAAAAAGCTCCAGGGTGCACATGAGGAGTCCAAGGCTATTACTAAGGCTCCTTCAATGAAATCAATCAAGCAACGGATGGAAAAAGATGTCGATGAAGTTGGAAAAGTTGCTCGTTGGATAAAGTCAAAAATTGAAGAACTTGACAAAGAGAATTTAGCAAATAGACAGAAGGCTGGTTGCGGAAAAGGAACAGGTGTAGATAGATCCCGAACAGCAACAACCCTTGCCTTGAAAAAGAAATTAAAGGACAAGATGGCTGAATTTCAGACTCTAAGGGAAACCATCCATCAAGAGTATCGTGATGTTGTCGAGAGGCGAGTTTTTACAGTGACGGGCGCAAGAGCTGATGAAGAGACAATTGAAAGATTAATCGAGACAGGGGACAGTGAACAAATTTTCCAAAAGGCAATCCAGGAACAAGGGCGAGGCCAGATAATGGACACTCTGGCCGAAATTCAAGAGCGACATGATGCTGTTAGAGATCTTGAGAAGAAGCTTCTCGATTTACAACAGATATTTATGGATATGGCGGTGCTGGTTGATGCACAAGGGGAATTGCTTGACAACATAGAAACCCAGGTATCAAGTGCAGTAGATCATGTACAGCAGGGGAATACCGCTCTTCAGAAGGCCAAGAAGCTACAAAAGAATTCGAGGAAATGGATGTGCATTGCGATTCTCATTCTTCTTATCATCGTTGCAATCATTGTGGTGGCGGTGTTCAAGCCGTGGCAGAGCAACAAGGGTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0031201 GO:0031224 GO:0032940 GO:0032991 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048278 GO:0048284 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 ko04721 map04130 map04721

Pfam Domains

Protein Families

SNARE Syntaxin

Protein Analysis

309

Amino Acids

34.86

Weight (kDa)

6.99

Isoelectric Point (pI)

38.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin	PF00804	36 - 195	5.3e-51	Syntaxin
SNARE	PF05739	243 - 295	6.1e-19	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006075)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03800 AT3G03800 AT5G08080 AT5G08080 AT5G08080 AT5G08080 AT5G08080
fragaria_vesca	FvH4_1g28350 FvH4_1g28350 FvH4_1g28350
malus_domestica	MD12G1006500.v1.1 MD14G1004100.v1.1
prunus_persica	Prupe.7G006600_v2.0.a1
pyrus_communis	pycom12g00480 pycom14g00190
rosa_chinensis	RchiOBHm_Chr3g0496741
rosa_laevigata	RLG00000022509
rosa_multiflora	Rmu_sc0000542.1_g000019
rosa_roxburghii	Rroxscaffold_6G00388750
rosa_rugosa	Rorug03G0286300 Rorug03G0286300 Rorug03G0286300 Rorug03G0286300
rosa_samantha	Rh3AG330900 Rh3BG366900 Rh3DG366700
rosa_wichuraiana	Rw3G028970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	18
AccB7I	CCANNNNNTGG	1 cut(s)	610
AccBSI	CCGCTC	1 cut(s)	794
AciI	CCGC	4 cut(s)	354, 704, 792, 890
AclWI	GGATC	2 cut(s)	25, 372
AcoI	YGGCCR	1 cut(s)	621
AcsI	RAATTY	7 cut(s)	91, 115, 325, 434, 567, 627, 823
AcuI	CTGAAG	1 cut(s)	782
AfaI	GTAC	1 cut(s)	777
AfiI	CCNNNNNNNGG	2 cut(s)	16, 610
AjnI	CCWGG	3 cut(s)	183, 585, 750
AloI	GAACNNNNNNTCC	2 cut(s)	555, 587
AluBI	AGCT	7 cut(s)	47, 160, 169, 181, 518, 670, 814
AluI	AGCT	7 cut(s)	47, 160, 169, 181, 518, 670, 814
Alw21I	GWGCWC	2 cut(s)	79, 193
Alw26I	GTCTC	2 cut(s)	521, 542
Alw44I	GTGCAC	1 cut(s)	189
AlwI	GGATC	2 cut(s)	25, 372
Ama87I	CYCGRG	1 cut(s)	36
AoxI	GGCC	3 cut(s)	601, 621, 804
ApaLI	GTGCAC	1 cut(s)	189
ApoI	RAATTY	7 cut(s)	91, 115, 325, 434, 567, 627, 823
AseI	ATTAAT	1 cut(s)	542
Asp700I	GAANNNNTTC	1 cut(s)	119
AspLEI	GCGC	1 cut(s)	512
AvaI	CYCGRG	1 cut(s)	36
BaeGI	GKGCMC	1 cut(s)	193
BaeI	ACNNNNGTAYC	2 cut(s)	738, 771
Bbv12I	GWGCWC	2 cut(s)	79, 193
BccI	CCATC	4 cut(s)	244, 421, 464, 468
BceAI	ACGGC	1 cut(s)	886
BciT130I	CCWGG	3 cut(s)	185, 587, 752
BcoDI	GTCTC	2 cut(s)	521, 542
BfaI	CTAG	1 cut(s)	71
BfuAI	ACCTGC	1 cut(s)	18
BglII	AGATCT	1 cut(s)	655
Bme1390I	CCNGG	3 cut(s)	185, 587, 752
BmeT110I	CYCGRG	1 cut(s)	36
BmiI	GGNNCC	1 cut(s)	222
BmrFI	CCNGG	3 cut(s)	185, 587, 752
BmsI	GCATC	2 cut(s)	634, 706
BpmI	CTGGAG	2 cut(s)	117, 167
BpuEI	CTTGAG	1 cut(s)	680
BsaJI	CCNNGG	5 cut(s)	35, 184, 204, 750, 902
BsaXI	ACNNNNNCTCC	2 cut(s)	205, 235
Bsc4I	CCNNNNNNNGG	2 cut(s)	16, 610
Bse3DI	GCAATG	1 cut(s)	843
BseBI	CCWGG	3 cut(s)	185, 587, 752
BseDI	CCNNGG	5 cut(s)	35, 184, 204, 750, 902
BseGI	GGATG	3 cut(s)	255, 456, 843
BseLI	CCNNNNNNNGG	2 cut(s)	16, 610
BseMI	GCAATG	1 cut(s)	843
BseRI	GAGGAG	1 cut(s)	212
BseSI	GKGCMC	1 cut(s)	193
BsgI	GTGCAG	1 cut(s)	783
BshFI	GGCC	3 cut(s)	603, 623, 806
BsiHKAI	GWGCWC	2 cut(s)	79, 193
BsiHKCI	CYCGRG	1 cut(s)	36
BslFI	GGGAC	1 cut(s)	569
BslI	CCNNNNNNNGG	2 cut(s)	16, 610
BsmAI	GTCTC	2 cut(s)	521, 542
BsmFI	GGGAC	1 cut(s)	569
BsnI	GGCC	3 cut(s)	603, 623, 806
BsoBI	CYCGRG	1 cut(s)	36
Bsp1286I	GDGCHC	2 cut(s)	79, 193
Bsp1407I	TGTACA	1 cut(s)	775
Bsp143I	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
BspACI	CCGC	4 cut(s)	354, 704, 792, 890
BspANI	GGCC	3 cut(s)	603, 623, 806
BspLI	GGNNCC	1 cut(s)	222
BspMI	ACCTGC	1 cut(s)	18
BspPI	GGATC	2 cut(s)	25, 372
BspQI	GCTCTTC	1 cut(s)	801
BsrBI	CCGCTC	1 cut(s)	794
BsrDI	GCAATG	1 cut(s)	843
BsrGI	TGTACA	1 cut(s)	775
BssECI	CCNNGG	5 cut(s)	35, 184, 204, 750, 902
BssMI	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
BssT1I	CCWWGG	1 cut(s)	204
Bst2UI	CCWGG	3 cut(s)	185, 587, 752
Bst4CI	ACNGT	2 cut(s)	502, 560
Bst6I	CTCTTC	2 cut(s)	519, 801
BstAUI	TGTACA	1 cut(s)	775
BstDEI	CTNAG	2 cut(s)	216, 446
BstDSI	CCRYGG	1 cut(s)	902
BstF5I	GGATG	3 cut(s)	255, 456, 843
BstHHI	GCGC	1 cut(s)	512
BstKTI	GATC	6 cut(s)	33, 58, 141, 380, 658, 772
BstMAI	GTCTC	2 cut(s)	521, 542
BstMBI	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
BstMWI	GCNNNNNNNGC	1 cut(s)	166
BstNI	CCWGG	3 cut(s)	185, 587, 752
BstNSI	RCATGY	1 cut(s)	83
BstSCI	CCNGG	3 cut(s)	183, 585, 750
BstSLI	GKGCMC	1 cut(s)	193
BstX2I	RGATCY	3 cut(s)	30, 377, 655
BstYI	RGATCY	3 cut(s)	30, 377, 655
BsuRI	GGCC	3 cut(s)	603, 623, 806
BtgI	CCRYGG	1 cut(s)	902
BtsCI	GGATG	3 cut(s)	255, 456, 843
BtsIMutI	CAGTG	2 cut(s)	507, 565
BveI	ACCTGC	1 cut(s)	18
CfoI	GCGC	1 cut(s)	512
Csp6I	GTAC	1 cut(s)	776
CspCI	CAANNNNNGTGG	2 cut(s)	864, 899
CviAII	CATG	4 cut(s)	80, 194, 641, 773
CviQI	GTAC	1 cut(s)	776
DdeI	CTNAG	2 cut(s)	216, 446
DpnI	GATC	6 cut(s)	32, 57, 140, 379, 657, 771
DpnII	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
EaeI	YGGCCR	1 cut(s)	621
Eam1104I	CTCTTC	2 cut(s)	519, 801
EarI	CTCTTC	2 cut(s)	519, 801
Eco130I	CCWWGG	1 cut(s)	204
Eco57I	CTGAAG	1 cut(s)	782
Eco88I	CYCGRG	1 cut(s)	36
EcoRI	GAATTC	2 cut(s)	91, 823
EcoRII	CCWGG	3 cut(s)	183, 585, 750
EcoT14I	CCWWGG	1 cut(s)	204
ErhI	CCWWGG	1 cut(s)	204
FaeI	CATG	4 cut(s)	83, 197, 644, 776
FaiI	YATR	7 cut(s)	81, 195, 642, 695, 701, 743, 774
FaqI	GGGAC	1 cut(s)	569
FatI	CATG	4 cut(s)	79, 193, 640, 772
FokI	GGATG	3 cut(s)	262, 443, 850
FspBI	CTAG	1 cut(s)	71
GlaI	GCGC	1 cut(s)	511
GsuI	CTGGAG	2 cut(s)	117, 167
HaeIII	GGCC	3 cut(s)	603, 623, 806
HhaI	GCGC	1 cut(s)	512
Hin1II	CATG	4 cut(s)	83, 197, 644, 776
Hin6I	GCGC	1 cut(s)	510
HinP1I	GCGC	1 cut(s)	510
HindIII	AAGCTT	4 cut(s)	45, 158, 167, 668
HinfI	GANTC	4 cut(s)	20, 200, 442, 850
Hpy166II	GTNNAC	2 cut(s)	191, 563
Hpy188I	TCNGA	2 cut(s)	441, 801
Hpy8I	GTNNAC	2 cut(s)	191, 563
HpyAV	CCTTC	3 cut(s)	234, 337, 796
HpyCH4III	ACNGT	2 cut(s)	502, 560
HpyCH4V	TGCA	5 cut(s)	191, 719, 764, 843, 875
HpyF10VI	GCNNNNNNNGC	1 cut(s)	166
HpyF3I	CTNAG	2 cut(s)	216, 446
Hsp92II	CATG	4 cut(s)	83, 197, 644, 776
HspAI	GCGC	1 cut(s)	510
Kzo9I	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
LguI	GCTCTTC	1 cut(s)	801
LmnI	GCTCC	3 cut(s)	74, 186, 226
LweI	GCATC	2 cut(s)	634, 706
MaeI	CTAG	1 cut(s)	71
MaeIII	GTNAC	1 cut(s)	502
MalI	GATC	6 cut(s)	32, 57, 140, 379, 657, 771
MbiI	CCGCTC	1 cut(s)	794
MboI	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
MboII	GAAGA	6 cut(s)	112, 320, 536, 676, 788, 851
MfeI	CAATTG	1 cut(s)	531
MflI	RGATCY	3 cut(s)	30, 377, 655
MhlI	GDGCHC	2 cut(s)	79, 193
MlyI	GAGTC	2 cut(s)	209, 436
MmeI	TCCRAC	2 cut(s)	253, 269
MnlI	CCTC	5 cut(s)	45, 190, 480, 593, 822
MroXI	GAANNNNTTC	1 cut(s)	119
MseI	TTAA	3 cut(s)	416, 542, 925
MslI	CAYNNNNRTG	1 cut(s)	884
MspR9I	CCNGG	3 cut(s)	185, 587, 752
MunI	CAATTG	1 cut(s)	531
MvaI	CCWGG	3 cut(s)	185, 587, 752
MwoI	GCNNNNNNNGC	1 cut(s)	166
NdeII	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
NlaIII	CATG	4 cut(s)	83, 197, 644, 776
NlaIV	GGNNCC	1 cut(s)	222
NmuCI	GTSAC	1 cut(s)	502
NspI	RCATGY	1 cut(s)	83
PciSI	GCTCTTC	1 cut(s)	801
PdmI	GAANNNNTTC	1 cut(s)	119
PfeI	GAWTC	2 cut(s)	20, 850
PflMI	CCANNNNNTGG	1 cut(s)	610
PfoI	TCCNGGA	1 cut(s)	585
PleI	GAGTC	2 cut(s)	208, 436
PpsI	GAGTC	2 cut(s)	208, 436
PshBI	ATTAAT	1 cut(s)	542
Psp6I	CCWGG	3 cut(s)	183, 585, 750
PspGI	CCWGG	3 cut(s)	183, 585, 750
PspN4I	GGNNCC	1 cut(s)	222
PsuI	RGATCY	3 cut(s)	30, 377, 655
RsaI	GTAC	1 cut(s)	777
RsaNI	GTAC	1 cut(s)	776
RseI	CAYNNNNRTG	1 cut(s)	884
SapI	GCTCTTC	1 cut(s)	801
SaqAI	TTAA	3 cut(s)	416, 542, 925
Sau3AI	GATC	6 cut(s)	30, 55, 138, 377, 655, 769
SchI	GAGTC	2 cut(s)	209, 436
ScrFI	CCNGG	3 cut(s)	185, 587, 752
SduI	GDGCHC	2 cut(s)	79, 193
SfaNI	GCATC	2 cut(s)	634, 706
SmiMI	CAYNNNNRTG	1 cut(s)	884
SmlI	CTYRAG	1 cut(s)	659
SmoI	CTYRAG	1 cut(s)	659
SsiI	CCGC	4 cut(s)	354, 704, 792, 890
SspMI	CTAG	1 cut(s)	71
StyD4I	CCNGG	3 cut(s)	183, 585, 750
StyI	CCWWGG	1 cut(s)	204
TaaI	ACNGT	2 cut(s)	502, 560
TaqI	TCGA	7 cut(s)	50, 141, 264, 483, 546, 675, 827
TatI	WGTACW	1 cut(s)	775
TfiI	GAWTC	2 cut(s)	20, 850
Tru1I	TTAA	3 cut(s)	416, 542, 925
Tru9I	TTAA	3 cut(s)	416, 542, 925
TscAI	CASTG	2 cut(s)	507, 565
TseFI	GTSAC	1 cut(s)	502
Tsp45I	GTSAC	1 cut(s)	502
TspDTI	ATGAA	5 cut(s)	17, 104, 245, 282, 537
TspGWI	ACGGA	2 cut(s)	32, 262
TspRI	CASTG	2 cut(s)	507, 565
Van91I	CCANNNNNTGG	1 cut(s)	610
VneI	GTGCAC	1 cut(s)	189
VspI	ATTAAT	1 cut(s)	542
XapI	RAATTY	7 cut(s)	91, 115, 325, 434, 567, 627, 823
XceI	RCATGY	1 cut(s)	83
XmnI	GAANNNNTTC	1 cut(s)	119
XspI	CTAG	1 cut(s)	71

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.