MD14G1030900.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009699
Physical Location & Seq
Forward (+)
2731115 .. 2732624
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1030900.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGAGGATTTACCACCTGGATATAGGTTCTACCCAACAGAGGAAGAGCTGATTTCGTTTTACTTGCAGAACAAACTTGGTAGGAGGAGTGAGGACTTGAACCGAGTTCTGGACCGAATCATACCCGTTGTTTTCATTTATGAGTTTAATCCATGGGAACTCCCACAGGTTTCGGGAGAGGTGAGCCATGGAGATCCAGAGCAGTGGTTCTTTTTCATCCCAAGACAAGAGAGTGAAGCTCGAGGTGGGAGACAGAGGCGACTCACAACAACATGGTATTGGAAAGAAACAGGATCTTCGAGCATTGTTTACTCTTCCAATTCCAATTACAAACGACCCATCGGCCACAAAAGAACCATGGGTTTCTACACTGGCAGAGCTCCGCATGGAAAGAAAACCGAGTGGATGATGAATGAATACAAAGCCATCGAAGTTCATGCAGATCATAATAATCAACTATCAATGGCAGCTTCTTCATCAAACCCTAGTACAAGTACTCCTGCTCCCACGTTAAGGCAAGAATTCAGCTTATGCCGAGTGTATAAGAAATCGAAATGCCTTAGGGAATTTGACAGACCACCTCAAGGGATTGAGATCACAAGAAACCCTAGCTTAAACATTCAAGCAGCTCCTGCTCAGGGTGCAAATCATCTGGATCAACATTCGACAACATCGAATAGGAACCCTCAGAATATGGGAGAAAGAACAAACTTAAGCTCACTGGAGAGTTCATCCTCAGGAGACCATGGCCTTCATTCCTCTCAACCAGAACAAAGCGGGACTTTGCTAATGGCCGTTGAAAATGAAGCTATTTGGGATTGGGACGAATTAATGGATAATTGGCTTTAA

Protein Analysis

283

Amino Acids

32.4

Weight (kDa)

6.01

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 5 - 141 2.6e-33 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 383, 777
AclWI GGATC 3 cut(s) 188, 301, 663
AcoI YGGCCR 2 cut(s) 343, 792
AcsI RAATTY 2 cut(s) 521, 566
AfaI GTAC 2 cut(s) 490, 496
AfiI CCNNNNNNNGG 5 cut(s) 40, 109, 513, 584, 638
AflII CTTAAG 1 cut(s) 712
AgsI TTSAA 3 cut(s) 100, 623, 800
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 9 cut(s) 49, 239, 380, 470, 528, 612, 629, 717, 809
AluI AGCT 9 cut(s) 49, 239, 380, 470, 528, 612, 629, 717, 809
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 2 cut(s) 244, 735
AlwI GGATC 3 cut(s) 188, 301, 663
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 3 cut(s) 343, 748, 792
ApeKI GCWGC 2 cut(s) 467, 626
ApoI RAATTY 2 cut(s) 521, 566
AseI ATTAAT 1 cut(s) 830
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 112
AxyI CCTNAGG 2 cut(s) 560, 736
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 479, 638
BccI CCATC 2 cut(s) 347, 434
BceAI ACGGC 1 cut(s) 779
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 18
BcoDI GTCTC 2 cut(s) 244, 735
BfaI CTAG 2 cut(s) 486, 609
BfrI CTTAAG 1 cut(s) 712
BisI GCNGC 2 cut(s) 468, 627
BlsI GCNGC 2 cut(s) 469, 628
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 683
BmrFI CCNGG 1 cut(s) 18
BplI GAGNNNNNCTC 2 cut(s) 223, 255
BpmI CTGGAG 1 cut(s) 743
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 567
BsaBI GATNNNNATC 1 cut(s) 593
BsaI GGTCTC 1 cut(s) 735
BsaJI CCNNGG 4 cut(s) 152, 187, 357, 745
BsaXI ACNNNNNCTCC 4 cut(s) 481, 487, 511, 517
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 109, 513, 584, 638
Bse1I ACTGG 2 cut(s) 376, 726
Bse21I CCTNAGG 2 cut(s) 560, 736
Bse8I GATNNNNATC 1 cut(s) 593
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 4 cut(s) 152, 187, 357, 745
BseGI GGATG 3 cut(s) 216, 411, 731
BseJI GATNNNNATC 1 cut(s) 593
BseLI CCNNNNNNNGG 5 cut(s) 40, 109, 513, 584, 638
BseMII CTCAG 3 cut(s) 650, 701, 750
BseNI ACTGG 2 cut(s) 376, 726
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 2 cut(s) 479, 638
BshFI GGCC 3 cut(s) 345, 750, 794
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 240
BslFI GGGAC 2 cut(s) 793, 836
BslI CCNNNNNNNGG 5 cut(s) 40, 109, 513, 584, 638
BsmAI GTCTC 2 cut(s) 244, 735
BsmFI GGGAC 2 cut(s) 793, 836
BsnI GGCC 3 cut(s) 345, 750, 794
Bso31I GGTCTC 1 cut(s) 735
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 5 cut(s) 193, 293, 442, 594, 655
Bsp19I CCATGG 4 cut(s) 152, 187, 357, 745
BspACI CCGC 2 cut(s) 383, 777
BspANI GGCC 3 cut(s) 345, 750, 794
BspCNI CTCAG 3 cut(s) 649, 700, 749
BspLI GGNNCC 1 cut(s) 683
BspPI GGATC 3 cut(s) 188, 301, 663
BspQI GCTCTTC 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 712
BspTNI GGTCTC 1 cut(s) 735
BsrI ACTGG 2 cut(s) 376, 726
BssECI CCNNGG 4 cut(s) 152, 187, 357, 745
BssMI GATC 5 cut(s) 193, 293, 442, 594, 655
BssT1I CCWWGG 4 cut(s) 152, 187, 357, 745
Bst2UI CCWGG 1 cut(s) 18
Bst6I CTCTTC 2 cut(s) 39, 319
BstAFI CTTAAG 1 cut(s) 712
BstAPI GCANNNNNTGC 1 cut(s) 632
BstDEI CTNAG 4 cut(s) 560, 636, 687, 736
BstDSI CCRYGG 4 cut(s) 152, 187, 357, 745
BstF5I GGATG 3 cut(s) 216, 411, 731
BstKTI GATC 5 cut(s) 196, 296, 445, 597, 658
BstMAI GTCTC 2 cut(s) 244, 735
BstMBI GATC 5 cut(s) 193, 293, 442, 594, 655
BstMWI GCNNNNNNNGC 2 cut(s) 632, 641
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 479, 638
BstX2I RGATCY 2 cut(s) 193, 293
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 2 cut(s) 193, 293
Bsu36I CCTNAGG 2 cut(s) 560, 736
BsuRI GGCC 3 cut(s) 345, 750, 794
BtgI CCRYGG 4 cut(s) 152, 187, 357, 745
BtsCI GGATG 3 cut(s) 216, 411, 731
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 3 cut(s) 209, 369, 719
CaiI CAGNNNCTG 1 cut(s) 632
Cfr13I GGNCC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 489, 495
CviAII CATG 7 cut(s) 153, 188, 273, 358, 386, 437, 746
CviQI GTAC 2 cut(s) 489, 495
DdeI CTNAG 4 cut(s) 560, 636, 687, 736
DpnI GATC 5 cut(s) 195, 295, 444, 596, 657
DpnII GATC 5 cut(s) 193, 293, 442, 594, 655
EaeI YGGCCR 2 cut(s) 343, 792
Eam1104I CTCTTC 2 cut(s) 39, 319
EarI CTCTTC 2 cut(s) 39, 319
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 4 cut(s) 152, 187, 357, 745
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 735
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 380
Eco81I CCTNAGG 2 cut(s) 560, 736
Eco88I CYCGRG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 16
EcoT14I CCWWGG 4 cut(s) 152, 187, 357, 745
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 4 cut(s) 152, 187, 357, 745
FaeI CATG 7 cut(s) 156, 191, 276, 361, 389, 440, 749
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FaqI GGGAC 2 cut(s) 793, 836
FatI CATG 7 cut(s) 152, 187, 272, 357, 385, 436, 745
FauI CCCGC 1 cut(s) 770
Fnu4HI GCNGC 2 cut(s) 468, 627
FokI GGATG 3 cut(s) 203, 418, 718
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 2 cut(s) 468, 627
FspBI CTAG 2 cut(s) 486, 609
GluI GCNGC 2 cut(s) 468, 627
GsuI CTGGAG 1 cut(s) 743
HaeIII GGCC 3 cut(s) 345, 750, 794
Hin1II CATG 7 cut(s) 156, 191, 276, 361, 389, 440, 749
HinfI GANTC 2 cut(s) 117, 261
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 1 cut(s) 690
Hpy188III TCNNGA 5 cut(s) 110, 174, 197, 653, 738
Hpy8I GTNNAC 1 cut(s) 310
HpyAV CCTTC 1 cut(s) 761
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 3 cut(s) 67, 440, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 641
HpyF3I CTNAG 4 cut(s) 560, 636, 687, 736
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 7 cut(s) 156, 191, 276, 361, 389, 440, 749
Kzo9I GATC 5 cut(s) 193, 293, 442, 594, 655
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 385, 508, 634
Lsp1109I GCAGC 2 cut(s) 479, 638
MaeI CTAG 2 cut(s) 486, 609
MaeII ACGT 1 cut(s) 509
MalI GATC 5 cut(s) 195, 295, 444, 596, 657
MboI GATC 5 cut(s) 193, 293, 442, 594, 655
MboII GAAGA 4 cut(s) 56, 288, 306, 465
MflI RGATCY 2 cut(s) 193, 293
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 6 cut(s) 319, 325, 521, 566, 827, 838
MlyI GAGTC 1 cut(s) 255
MseI TTAA 6 cut(s) 147, 512, 614, 713, 830, 847
MspCI CTTAAG 1 cut(s) 712
MspR9I CCNGG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 632, 641
NcoI CCATGG 4 cut(s) 152, 187, 357, 745
NdeII GATC 5 cut(s) 193, 293, 442, 594, 655
NlaIII CATG 7 cut(s) 156, 191, 276, 361, 389, 440, 749
NlaIV GGNNCC 1 cut(s) 683
NmeAIII GCCGAG 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 671
PfeI GAWTC 1 cut(s) 117
PkrI GCNGC 2 cut(s) 469, 628
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PshBI ATTAAT 1 cut(s) 830
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 683
PspPI GGNCC 1 cut(s) 112
PspXI VCTCGAGB 1 cut(s) 240
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 2 cut(s) 193, 293
RsaI GTAC 2 cut(s) 490, 496
RsaNI GTAC 2 cut(s) 489, 495
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 6 cut(s) 147, 512, 614, 713, 830, 847
SatI GCNGC 2 cut(s) 468, 627
Sau3AI GATC 5 cut(s) 193, 293, 442, 594, 655
Sau96I GGNCC 1 cut(s) 112
ScaI AGTACT 1 cut(s) 496
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 1 cut(s) 382
Sfr274I CTCGAG 1 cut(s) 240
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 1 cut(s) 240
SmlI CTYRAG 3 cut(s) 240, 582, 712
SmoI CTYRAG 3 cut(s) 240, 582, 712
Sse9I AATT 6 cut(s) 319, 325, 521, 566, 827, 838
SsiI CCGC 2 cut(s) 383, 777
SspMI CTAG 2 cut(s) 486, 609
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 16
StyI CCWWGG 4 cut(s) 152, 187, 357, 745
TaiI ACGT 1 cut(s) 512
TaqI TCGA 6 cut(s) 241, 299, 429, 551, 665, 674
TaqII GACCGA 1 cut(s) 129
TasI AATT 6 cut(s) 319, 325, 521, 566, 827, 838
TatI WGTACW 2 cut(s) 488, 494
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 6 cut(s) 147, 512, 614, 713, 830, 847
Tru9I TTAA 6 cut(s) 147, 512, 614, 713, 830, 847
TscAI CASTG 3 cut(s) 209, 376, 726
TseI GCWGC 2 cut(s) 467, 626
TspDTI ATGAA 9 cut(s) 124, 205, 425, 425, 429, 465, 720, 743, 819
TspRI CASTG 3 cut(s) 209, 376, 726
Vha464I CTTAAG 1 cut(s) 712
VpaK11BI GGWCC 1 cut(s) 112
VspI ATTAAT 1 cut(s) 830
XapI RAATTY 2 cut(s) 521, 566
XhoI CTCGAG 1 cut(s) 240
XspI CTAG 2 cut(s) 486, 609
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.