MD14G1031000.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009699
Physical Location & Seq
Forward (+)
2757019 .. 2759158
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1031000.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGATTGTTGTGTTTTGTGTTGGGAAGAAGAGGTTGCGGGAGAGGTGAGCCATGGAGATTCAGAGCAGTGGTTCTTTTTCATCCCAAGACCAGAGAGTGAAGCTCGAGGAGGGAGACCGAGACGACTCACAACAATTGGGTATTGGAAAGCATCAGGATCTCCGAGCACTGTGTACTCTTCCAATTCCAATTACAATCGTCCCATCGGCCACAAAAGAACCATGGGTTTCTACATTGGCAGAGCTCCCCGTGGAAAGAAAACCGAGTGGATGATGAATGAATACAAAGCCATCGAAGCTCATGCTGATCATAATAATCAACTATCAATGGCAGCTTCTTCATCAATCCCTAGAACAAGTACTCCTTCTGCTCCCACGTTAAGGCAAGAATTCAGCTTATGCCGAGTGTACAGGAAATCAAAATCCCTTAGGGCATATGACAGAAGACATCCAGGGATTGAGATCACAAGAAACCCTAGCTTAAACATTCAAGCAGCTCCTGCTCAGGGTGCAAATCATCTGAATCAATGTTCGACAACGTCGAATAGGAACCCTCAGAATATGGGACAGAGAACAAACTTAAGCTCACTGGAGAGTTCATCCTCAGGAGGCCATGCCTCTCAATCCTCTCAACCCGAACAAAGTGGGATTTTGCCAATGGCCGTTGATGATGAAGCTATTTGGGATTGGGACGAATTAATGGACAACTGGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

239

Amino Acids

26.68

Weight (kDa)

7.7

Isoelectric Point (pI)

60.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 17 - 96 5.2e-18 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 38
AclWI GGATC 1 cut(s) 166
AcoI YGGCCR 2 cut(s) 208, 660
AcsI RAATTY 1 cut(s) 389
AfaI GTAC 3 cut(s) 176, 361, 410
AfiI CCNNNNNNNGG 2 cut(s) 381, 506
AflII CTTAAG 1 cut(s) 580
AgsI TTSAA 1 cut(s) 491
AjnI CCWGG 1 cut(s) 451
AloI GAACNNNNNNTCC 2 cut(s) 346, 378
AluBI AGCT 9 cut(s) 104, 245, 299, 335, 396, 480, 497, 585, 677
AluI AGCT 9 cut(s) 104, 245, 299, 335, 396, 480, 497, 585, 677
Alw21I GWGCWC 2 cut(s) 170, 247
Alw26I GTCTC 2 cut(s) 109, 115
AlwI GGATC 1 cut(s) 166
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 3 cut(s) 208, 610, 660
ApeKI GCWGC 2 cut(s) 332, 494
ApoI RAATTY 1 cut(s) 389
AseI ATTAAT 1 cut(s) 698
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 105
AxyI CCTNAGG 2 cut(s) 428, 604
BanII GRGCYC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 2 cut(s) 170, 247
BbvI GCAGC 2 cut(s) 344, 506
BccI CCATC 2 cut(s) 212, 299
BceAI ACGGC 1 cut(s) 647
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 1 cut(s) 453
BclI TGATCA 1 cut(s) 307
BcoDI GTCTC 2 cut(s) 109, 115
BfaI CTAG 2 cut(s) 351, 477
BfrI CTTAAG 1 cut(s) 580
BisI GCNGC 2 cut(s) 333, 495
BlsI GCNGC 2 cut(s) 334, 496
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 453
BmeT110I CYCGRG 1 cut(s) 105
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 451
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 461
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 4 cut(s) 52, 222, 250, 452
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 381, 506
Bse1I ACTGG 2 cut(s) 594, 713
Bse21I CCTNAGG 2 cut(s) 428, 604
Bse8I GATNNNNATC 1 cut(s) 461
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 4 cut(s) 52, 222, 250, 452
BseGI GGATG 4 cut(s) 81, 276, 448, 599
BseJI GATNNNNATC 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 381, 506
BseMII CTCAG 3 cut(s) 518, 569, 618
BseNI ACTGG 2 cut(s) 594, 713
BseRI GAGGAG 1 cut(s) 123
BseXI GCAGC 2 cut(s) 344, 506
BshFI GGCC 3 cut(s) 210, 612, 662
BsiHKAI GWGCWC 2 cut(s) 170, 247
BsiHKCI CYCGRG 1 cut(s) 105
BslFI GGGAC 3 cut(s) 186, 579, 704
BslI CCNNNNNNNGG 2 cut(s) 381, 506
BsmAI GTCTC 2 cut(s) 109, 115
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 3 cut(s) 186, 579, 704
BsnI GGCC 3 cut(s) 210, 612, 662
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 2 cut(s) 170, 247
Bsp1407I TGTACA 1 cut(s) 408
Bsp143I GATC 3 cut(s) 158, 307, 462
Bsp19I CCATGG 2 cut(s) 52, 222
BspACI CCGC 1 cut(s) 38
BspANI GGCC 3 cut(s) 210, 612, 662
BspCNI CTCAG 3 cut(s) 517, 568, 617
BspLI GGNNCC 1 cut(s) 551
BspPI GGATC 1 cut(s) 166
BspTI CTTAAG 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 408
BsrI ACTGG 2 cut(s) 594, 713
BssECI CCNNGG 4 cut(s) 52, 222, 250, 452
BssMI GATC 3 cut(s) 158, 307, 462
BssT1I CCWWGG 2 cut(s) 52, 222
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 24, 184
BstAFI CTTAAG 1 cut(s) 580
BstAPI GCANNNNNTGC 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 408
BstDEI CTNAG 4 cut(s) 428, 504, 555, 604
BstDSI CCRYGG 3 cut(s) 52, 222, 250
BstF5I GGATG 4 cut(s) 81, 276, 448, 599
BstKTI GATC 3 cut(s) 161, 310, 465
BstMAI GTCTC 2 cut(s) 109, 115
BstMBI GATC 3 cut(s) 158, 307, 462
BstMWI GCNNNNNNNGC 3 cut(s) 296, 500, 509
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstV1I GCAGC 2 cut(s) 344, 506
BstV2I GAAGAC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
Bsu36I CCTNAGG 2 cut(s) 428, 604
BsuRI GGCC 3 cut(s) 210, 612, 662
BtgI CCRYGG 3 cut(s) 52, 222, 250
BtsCI GGATG 4 cut(s) 81, 276, 448, 599
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 74, 168, 587
CaiI CAGNNNCTG 1 cut(s) 500
Csp6I GTAC 3 cut(s) 175, 360, 409
CviAII CATG 4 cut(s) 53, 223, 302, 614
CviQI GTAC 3 cut(s) 175, 360, 409
DdeI CTNAG 4 cut(s) 428, 504, 555, 604
DpnI GATC 3 cut(s) 160, 309, 464
DpnII GATC 3 cut(s) 158, 307, 462
EaeI YGGCCR 2 cut(s) 208, 660
Eam1104I CTCTTC 2 cut(s) 24, 184
EarI CTCTTC 2 cut(s) 24, 184
Ecl136II GAGCTC 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 52, 222
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 245
Eco81I CCTNAGG 2 cut(s) 428, 604
Eco88I CYCGRG 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 2 cut(s) 52, 222
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 2 cut(s) 52, 222
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 4 cut(s) 56, 226, 305, 617
FaiI YATR 9 cut(s) 54, 224, 303, 312, 400, 436, 438, 563, 615
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 3 cut(s) 186, 579, 704
FatI CATG 4 cut(s) 52, 222, 301, 613
FauI CCCGC 1 cut(s) 31
FauNDI CATATG 1 cut(s) 436
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 333, 495
FokI GGATG 4 cut(s) 68, 283, 435, 586
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 2 cut(s) 333, 495
FspBI CTAG 2 cut(s) 351, 477
GluI GCNGC 2 cut(s) 333, 495
GsuI CTGGAG 1 cut(s) 611
HaeIII GGCC 3 cut(s) 210, 612, 662
Hin1II CATG 4 cut(s) 56, 226, 305, 617
HinfI GANTC 3 cut(s) 59, 126, 523
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 175, 409
Hpy188I TCNGA 4 cut(s) 64, 165, 522, 558
Hpy188III TCNNGA 2 cut(s) 156, 606
Hpy8I GTNNAC 2 cut(s) 175, 409
Hpy99I CGWCG 1 cut(s) 544
HpyAV CCTTC 1 cut(s) 375
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 377, 539
HpyCH4V TGCA 1 cut(s) 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 500, 509
HpyF3I CTNAG 4 cut(s) 428, 504, 555, 604
HpySE526I ACGT 2 cut(s) 377, 539
Hsp92II CATG 4 cut(s) 56, 226, 305, 617
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 158, 307, 462
LmnI GCTCC 3 cut(s) 250, 376, 502
Lsp1109I GCAGC 2 cut(s) 344, 506
LweI GCATC 1 cut(s) 161
MaeI CTAG 2 cut(s) 351, 477
MaeII ACGT 2 cut(s) 377, 539
MalI GATC 3 cut(s) 160, 309, 464
MboI GATC 3 cut(s) 158, 307, 462
MboII GAAGA 5 cut(s) 38, 41, 171, 330, 456
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 2 cut(s) 170, 247
MluCI AATT 5 cut(s) 135, 184, 190, 389, 695
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 9 cut(s) 25, 37, 101, 104, 564, 602, 613, 628, 637
MseI TTAA 5 cut(s) 380, 482, 581, 698, 715
MspCI CTTAAG 1 cut(s) 580
MspR9I CCNGG 1 cut(s) 453
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 3 cut(s) 296, 500, 509
NcoI CCATGG 2 cut(s) 52, 222
NdeI CATATG 1 cut(s) 436
NdeII GATC 3 cut(s) 158, 307, 462
NlaIII CATG 4 cut(s) 56, 226, 305, 617
NlaIV GGNNCC 1 cut(s) 551
NmeAIII GCCGAG 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 539
PfeI GAWTC 2 cut(s) 59, 523
PflFI GACNNNGTC 1 cut(s) 538
PkrI GCNGC 2 cut(s) 334, 496
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PshBI ATTAAT 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 551
PspXI VCTCGAGB 1 cut(s) 105
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 158
PsyI GACNNNGTC 1 cut(s) 538
RsaI GTAC 3 cut(s) 176, 361, 410
RsaNI GTAC 3 cut(s) 175, 360, 409
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 5 cut(s) 380, 482, 581, 698, 715
SatI GCNGC 2 cut(s) 333, 495
Sau3AI GATC 3 cut(s) 158, 307, 462
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 2 cut(s) 170, 247
SfaNI GCATC 1 cut(s) 161
Sfr274I CTCGAG 1 cut(s) 105
SlaI CTCGAG 1 cut(s) 105
SmlI CTYRAG 2 cut(s) 105, 580
SmoI CTYRAG 2 cut(s) 105, 580
Sse9I AATT 5 cut(s) 135, 184, 190, 389, 695
SsiI CCGC 1 cut(s) 38
SspMI CTAG 2 cut(s) 351, 477
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 2 cut(s) 52, 222
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 380, 542
TaqI TCGA 4 cut(s) 106, 294, 533, 542
TaqII GACCGA 1 cut(s) 133
TasI AATT 5 cut(s) 135, 184, 190, 389, 695
TatI WGTACW 3 cut(s) 174, 359, 408
TfiI GAWTC 2 cut(s) 59, 523
Tru1I TTAA 5 cut(s) 380, 482, 581, 698, 715
Tru9I TTAA 5 cut(s) 380, 482, 581, 698, 715
TscAI CASTG 3 cut(s) 74, 175, 594
TseI GCWGC 2 cut(s) 332, 494
TspDTI ATGAA 6 cut(s) 70, 290, 294, 330, 588, 687
TspRI CASTG 3 cut(s) 74, 175, 594
Tth111I GACNNNGTC 1 cut(s) 538
Vha464I CTTAAG 1 cut(s) 580
VspI ATTAAT 1 cut(s) 698
XapI RAATTY 1 cut(s) 389
XhoI CTCGAG 1 cut(s) 105
XspI CTAG 2 cut(s) 351, 477
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.