RchiOBHm_Chr3g0474741
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009699
Physical Location & Seq
Forward (+)
20484641 .. 20486763
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44031

Sequence Viewer

Length: 876 bp
ATGGAAGATTTTCCACCTGGGTTTAGATTCTACCCAACAGAGGAAGAACTAGTTTCATTTTATCTGCCCCACAAACTAGATGGAAGGAGGGAGGACGTGAACCGGGTTATGGACCGGATCATACCTGTGCTTGACATATATGAGTTTAATCCTTGGGATCTCCCACGAAATTCAGGAGAAATGTGCCATGGAGATCAAGAGCAGTGGTTCTTCTTCATCCCAAGACAAGAGAGTGAAGCACGGGGAGGGAGACCTAAACGACTCACTACTACTGGATATTGGAAGGCAACTGGTTCTCCAAGTTATGTTTTCTCTTCTAGTTCCAACACTAATCATGGCGCCATCGGCCTCAAAAGAACCATGGTCTTCTACACTGGTCGAGCTCCCAACGGAAGCAAAACCGAGTGGAAGATGAATGAGTATAAAGCCATTGATGGAGACAATCAACTTCAAGCACCTTCATCATCAAACGCCGCACCTTCTCCTCCGTTAAGGCAAGAATTCAGTTTATGCCGAGTGTACAAGAAATCGAAATGCTTAAGGGCATTTGACAGACGACCTTCAGGCACTGGGGATATCATGCGCAGACCTACAGTTACAGTTCATTTACCAGCTGTGCCGCAGTTAGCAGATACAGATCATCAGGAGGGTTCTACTTATCCAACATCGATATCTCATGCTCGGAACATCATCCCAGCTGCTCCAAATTCATATGATGTGGAAGTGGAGAGAAGAAGTCTAAGCTCATCTGGGAGTTCGTCCTCAGGAGACCTTGGCCAACCCTCTCTATCTGGACAGAGCACTGGAAGCTTGCCCATGGCCGTGGACAATGGTGAACCTCTATGGGAACTGGACCAACTAGATTGGTTTAATTAA

Protein Analysis

291

Amino Acids

32.66

Weight (kDa)

5.91

Isoelectric Point (pI)

64.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 5 - 142 4e-37 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 584
AccB1I GGYRCC 1 cut(s) 338
AciI CCGC 2 cut(s) 474, 620
AclWI GGATC 2 cut(s) 125, 165
AcoI YGGCCR 2 cut(s) 775, 819
AcsI RAATTY 3 cut(s) 169, 500, 706
AcuI CTGAAG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 1 cut(s) 521
AfiI CCNNNNNNNGG 2 cut(s) 40, 109
AflII CTTAAG 1 cut(s) 538
AgsI TTSAA 1 cut(s) 452
AhlI ACTAGT 1 cut(s) 49
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 5 cut(s) 383, 614, 698, 744, 810
AluI AGCT 5 cut(s) 383, 614, 698, 744, 810
Alw21I GWGCWC 2 cut(s) 385, 803
Alw26I GTCTC 3 cut(s) 244, 432, 762
AlwI GGATC 2 cut(s) 125, 165
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 3 cut(s) 346, 775, 819
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 3 cut(s) 169, 500, 706
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 2 cut(s) 341, 585
AspS9I GGNCC 2 cut(s) 112, 853
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 1 cut(s) 845
AvaII GGWCC 2 cut(s) 112, 853
AxyI CCTNAGG 1 cut(s) 763
BalI TGGCCA 1 cut(s) 777
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 2 cut(s) 385, 803
BbvI GCAGC 1 cut(s) 685
BccI CCATC 3 cut(s) 74, 350, 428
BceAI ACGGC 1 cut(s) 806
BciT130I CCWGG 1 cut(s) 18
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 3 cut(s) 244, 432, 762
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 4 cut(s) 50, 77, 318, 860
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 342
BfrI CTTAAG 1 cut(s) 538
BisI GCNGC 3 cut(s) 474, 620, 699
BlsI GCNGC 3 cut(s) 475, 621, 700
Bme1390I CCNGG 2 cut(s) 18, 104
Bme18I GGWCC 2 cut(s) 112, 853
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 112, 853
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 18, 104
BmrI ACTGGG 1 cut(s) 579
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 1 cut(s) 358
BpuMI CCSGG 1 cut(s) 104
Bsa29I ATCGAT 1 cut(s) 668
BsaBI GATNNNNATC 1 cut(s) 636
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 2 cut(s) 244, 762
BsaJI CCNNGG 7 cut(s) 17, 152, 187, 360, 772, 816, 822
BsaWI WCCGGW 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 109
Bse1I ACTGG 6 cut(s) 277, 295, 379, 574, 808, 855
Bse21I CCTNAGG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 1 cut(s) 18
BseCI ATCGAT 1 cut(s) 668
BseDI CCNNGG 7 cut(s) 17, 152, 187, 360, 772, 816, 822
BseGI GGATG 2 cut(s) 216, 690
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 40, 109
BseMII CTCAG 1 cut(s) 777
BseNI ACTGG 6 cut(s) 277, 295, 379, 574, 808, 855
BseRI GAGGAG 1 cut(s) 474
BseXI GCAGC 1 cut(s) 685
BseYI CCCAGC 1 cut(s) 694
BshFI GGCC 3 cut(s) 348, 777, 821
BshNI GGYRCC 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 668
BsiHKAI GWGCWC 2 cut(s) 385, 803
BsiSI CCGG 2 cut(s) 103, 115
BslI CCNNNNNNNGG 2 cut(s) 40, 109
BsmAI GTCTC 3 cut(s) 244, 432, 762
BsnI GGCC 3 cut(s) 348, 777, 821
Bso31I GGTCTC 2 cut(s) 244, 762
Bsp1286I GDGCHC 2 cut(s) 385, 803
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 4 cut(s) 117, 157, 193, 637
Bsp19I CCATGG 3 cut(s) 187, 360, 816
BspACI CCGC 2 cut(s) 474, 620
BspANI GGCC 3 cut(s) 348, 777, 821
BspCNI CTCAG 1 cut(s) 776
BspDI ATCGAT 1 cut(s) 668
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 2 cut(s) 125, 165
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 538
BspTNI GGTCTC 2 cut(s) 244, 762
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 6 cut(s) 277, 295, 379, 574, 808, 855
BssECI CCNNGG 7 cut(s) 17, 152, 187, 360, 772, 816, 822
BssMI GATC 4 cut(s) 117, 157, 193, 637
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 5 cut(s) 152, 187, 360, 772, 816
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 595, 601
Bst6I CTCTTC 1 cut(s) 319
BstACI GRCGYC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 538
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 1 cut(s) 812
BstDEI CTNAG 2 cut(s) 740, 763
BstDSI CCRYGG 4 cut(s) 187, 360, 816, 822
BstF5I GGATG 2 cut(s) 216, 690
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 2 cut(s) 341, 585
BstKTI GATC 4 cut(s) 120, 160, 196, 640
BstMAI GTCTC 3 cut(s) 244, 432, 762
BstMBI GATC 4 cut(s) 117, 157, 193, 637
BstMWI GCNNNNNNNGC 2 cut(s) 345, 807
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 2 cut(s) 16, 102
BstSFI CTRYAG 1 cut(s) 591
BstV1I GCAGC 1 cut(s) 685
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 823
BstYI RGATCY 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 668
Bsu36I CCTNAGG 1 cut(s) 763
BsuRI GGCC 3 cut(s) 348, 777, 821
BsuTUI ATCGAT 1 cut(s) 668
BtgI CCRYGG 4 cut(s) 187, 360, 816, 822
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 2 cut(s) 216, 690
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 4 cut(s) 209, 372, 567, 801
Cac8I GCNNGC 1 cut(s) 812
CaiI CAGNNNCTG 1 cut(s) 569
CfoI GCGC 2 cut(s) 341, 585
Cfr13I GGNCC 2 cut(s) 112, 853
ClaI ATCGAT 1 cut(s) 668
Csp6I GTAC 1 cut(s) 520
CspCI CAANNNNNGTGG 2 cut(s) 185, 220
CviAII CATG 6 cut(s) 188, 335, 361, 580, 677, 817
CviJI RGCY 9 cut(s) 348, 383, 428, 614, 698, 744, 777, 810, 821
CviKI_1 RGCY 9 cut(s) 348, 383, 428, 614, 698, 744, 777, 810, 821
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 2 cut(s) 740, 763
DinI GGCGCC 1 cut(s) 340
DpnI GATC 4 cut(s) 119, 159, 195, 639
DpnII GATC 4 cut(s) 117, 157, 193, 637
EaeI YGGCCR 2 cut(s) 775, 819
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 383
Eco130I CCWWGG 5 cut(s) 152, 187, 360, 772, 816
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 2 cut(s) 244, 762
Eco32I GATATC 2 cut(s) 577, 672
Eco47I GGWCC 2 cut(s) 112, 853
Eco53kI GAGCTC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 546
Eco81I CCTNAGG 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 2 cut(s) 577, 672
EcoT14I CCWWGG 5 cut(s) 152, 187, 360, 772, 816
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 5 cut(s) 152, 187, 360, 772, 816
FaeI CATG 6 cut(s) 191, 338, 364, 583, 680, 820
FatI CATG 6 cut(s) 187, 334, 360, 579, 676, 816
FauNDI CATATG 1 cut(s) 712
Fnu4HI GCNGC 3 cut(s) 474, 620, 699
FokI GGATG 2 cut(s) 203, 677
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 3 cut(s) 474, 620, 699
FspBI CTAG 4 cut(s) 50, 77, 318, 860
FspI TGCGCA 1 cut(s) 584
GlaI GCGC 2 cut(s) 340, 584
GluI GCNGC 3 cut(s) 474, 620, 699
GsaI CCCAGC 1 cut(s) 698
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 3 cut(s) 348, 777, 821
HapII CCGG 2 cut(s) 103, 115
HhaI GCGC 2 cut(s) 341, 585
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 6 cut(s) 191, 338, 364, 583, 680, 820
Hin6I GCGC 2 cut(s) 339, 583
HinP1I GCGC 2 cut(s) 339, 583
HindIII AAGCTT 1 cut(s) 808
HinfI GANTC 2 cut(s) 27, 261
HpaII CCGG 2 cut(s) 103, 115
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 4 cut(s) 100, 520, 826, 836
Hpy188I TCNGA 1 cut(s) 684
Hpy188III TCNNGA 5 cut(s) 174, 197, 644, 765, 792
Hpy8I GTNNAC 4 cut(s) 100, 520, 826, 836
HpyAV CCTTC 5 cut(s) 78, 277, 468, 489, 570
HpyCH4III ACNGT 2 cut(s) 595, 601
HpyCH4IV ACGT 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 2 cut(s) 345, 807
HpyF3I CTNAG 2 cut(s) 740, 763
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 6 cut(s) 191, 338, 364, 583, 680, 820
HspAI GCGC 2 cut(s) 339, 583
KasI GGCGCC 1 cut(s) 338
Kzo9I GATC 4 cut(s) 117, 157, 193, 637
LmnI GCTCC 2 cut(s) 388, 706
Lsp1109I GCAGC 1 cut(s) 685
MaeI CTAG 4 cut(s) 50, 77, 318, 860
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 595
MalI GATC 4 cut(s) 119, 159, 195, 639
MboI GATC 4 cut(s) 117, 157, 193, 637
MboII GAAGA 8 cut(s) 17, 56, 202, 205, 306, 358, 421, 744
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 385, 803
MlsI TGGCCA 1 cut(s) 777
MluCI AATT 4 cut(s) 169, 500, 706, 871
MluNI TGGCCA 1 cut(s) 777
Mly113I GGCGCC 1 cut(s) 339
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 2 cut(s) 348, 686
Mox20I TGGCCA 1 cut(s) 777
MroXI GAANNNNTTC 1 cut(s) 9
MscI TGGCCA 1 cut(s) 777
MseI TTAA 5 cut(s) 147, 491, 539, 870, 874
MslI CAYNNNNRTG 2 cut(s) 125, 821
Msp20I TGGCCA 1 cut(s) 777
MspA1I CMGCKG 2 cut(s) 614, 698
MspCI CTTAAG 1 cut(s) 538
MspI CCGG 2 cut(s) 103, 115
MspR9I CCNGG 2 cut(s) 18, 104
MvaI CCWGG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 345, 807
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 1 cut(s) 104
NcoI CCATGG 3 cut(s) 187, 360, 816
NdeI CATATG 1 cut(s) 712
NdeII GATC 4 cut(s) 117, 157, 193, 637
NlaIII CATG 6 cut(s) 191, 338, 364, 583, 680, 820
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 539
NsbI TGCGCA 1 cut(s) 584
PacI TTAATTAA 1 cut(s) 874
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 27
PkrI GCNGC 3 cut(s) 475, 621, 700
PleI GAGTC 1 cut(s) 255
PluTI GGCGCC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 385
Psp6I CCWGG 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 2 cut(s) 112, 853
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 157
PvuII CAGCTG 2 cut(s) 614, 698
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
RseI CAYNNNNRTG 2 cut(s) 125, 821
SacI GAGCTC 1 cut(s) 385
SaqAI TTAA 5 cut(s) 147, 491, 539, 870, 874
SatI GCNGC 3 cut(s) 474, 620, 699
Sau3AI GATC 4 cut(s) 117, 157, 193, 637
Sau96I GGNCC 2 cut(s) 112, 853
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 2 cut(s) 18, 104
SduI GDGCHC 2 cut(s) 385, 803
SfcI CTRYAG 1 cut(s) 591
SfoI GGCGCC 1 cut(s) 340
SinI GGWCC 2 cut(s) 112, 853
SmiMI CAYNNNNRTG 2 cut(s) 125, 821
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
SpeI ACTAGT 1 cut(s) 49
Sse9I AATT 4 cut(s) 169, 500, 706, 871
SsiI CCGC 2 cut(s) 474, 620
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 4 cut(s) 50, 77, 318, 860
SstI GAGCTC 1 cut(s) 385
StyD4I CCNGG 2 cut(s) 16, 102
StyI CCWWGG 5 cut(s) 152, 187, 360, 772, 816
TaaI ACNGT 2 cut(s) 595, 601
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 379, 530, 668
TasI AATT 4 cut(s) 169, 500, 706, 871
TatI WGTACW 1 cut(s) 519
TauI GCSGC 2 cut(s) 476, 622
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 5 cut(s) 147, 491, 539, 870, 874
Tru9I TTAA 5 cut(s) 147, 491, 539, 870, 874
TscAI CASTG 4 cut(s) 209, 379, 574, 808
TseI GCWGC 1 cut(s) 698
TspDTI ATGAA 6 cut(s) 45, 205, 428, 450, 593, 699
TspGWI ACGGA 2 cut(s) 405, 477
TspRI CASTG 4 cut(s) 209, 379, 574, 808
Vha464I CTTAAG 1 cut(s) 538
VpaK11BI GGWCC 2 cut(s) 112, 853
XapI RAATTY 3 cut(s) 169, 500, 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 4 cut(s) 50, 77, 318, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.