MD14G1039000.v1.1

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011263
Physical Location & Seq
Reverse (-)
3581232 .. 3583955
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1039000.v1.1.491

Sequence Viewer

Length: 984 bp
ATGAGTAAGGTTTGCCTTCCGTTTTCGTTTCCTTTTTACAAGAAGGAACCCCACGTCGCTTTTTATCCCTCCTTCCTTTGCCCTTTAATCCCCAGGAATCAAATTCCCCACGAATTAAACTCTTTTCCCTCACAAATCAAAAGAAGAAGAAGAAGAACAGAAAAAGAAAAACAGAAGAGAATGGGGCTGCTCTCCAACAGGGTGGATAAGGGGAGCTTGAAGCCAGGGGATCACATCTACTCTTGGAGGACTGCTTATATCTACGCCCATCACGGCATCTATGTTGGGAATGACACAGTCATTCATTTTACCAGACGTGGACAAGAAGTAGGAACGGGGACGATGCTGGATCTTCTCTTGGTTAGCTCAGGGCCAGCCCAACCTCGACAACCCTGCCAAATTTGCACTCCACGGGAAGAGGGCAGCGGTGTTCTCTCCTCATGCCTGAACTGCTTTCTTGCAGGTGGCATCTTGTATCGTTTTGAATATGCTGTCAACCCTGCTCTTTTTATTGCAAAAGCACGCGGCGGAACCTGTACCCTTGCAGTCTCAGACCCAAACGATGTTGTGGTCCATCGAGCAAAATACCTGTTTGAGAATGGCTTTGGTTGCTACAACGTGTTCAAGAACAACTGTGAAGACTTTGCTATTTACTGCAAAACGGGACTGCTTGTTCTGGATCAAGGAACAATCGGGCAAAGTGGTCAAGCAGTATCCATCATAGGGGGACCTCTTGCAGCTGTTTTGTCCACGCCATTGCGTCTTGTCACAACCAATGTTTATGGGATGGCCGCAACTGCCATTGGAGTCTACTGTGCTAGTAGGTATGCTGCTGACATTGGCATGAGGAGGGATGTAGTGAAGGTGTCTGTCGAGGATATGACAAGGAGAGTGGGAGCTGGCCTGCTCCAGGTGGCCGAGGCCCAAATCACAGCGGCCCCAATCACGGCCCTTGCAGGAGGTTCTTCTAACCTTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

328

Amino Acids

35.66

Weight (kDa)

9.3

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 73 - 224 2.2e-27 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06370
fragaria_vesca FvH4_6g20310 FvH4_6g20310 FvH4_6g20310
malus_domestica MD14G1039000.v1.1
prunus_persica Prupe.7G094600_v2.0.a1
pyrus_communis pycom14g03350
rosa_chinensis RchiOBHm_Chr3g0475931
rosa_laevigata RLG00000023799
rosa_multiflora Rmu_co8434183.1_g000001 Rmu_sc0000596.1_g000004 Rmu_sc0009168.1_g000002
rosa_roxburghii Rroxscaffold_6G00405720
rosa_rugosa Rorug03G0151900 Rorug03G0152000
rosa_samantha Rh3AG202900 Rh3BG232700 Rh3CG228200 Rh3DG228400
rosa_wichuraiana Rw3G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 1 cut(s) 452
AccI GTMKAC 1 cut(s) 810
AccII CGCG 1 cut(s) 525
AciI CCGC 5 cut(s) 426, 525, 528, 792, 935
AclWI GGATC 3 cut(s) 237, 357, 687
AcoI YGGCCR 2 cut(s) 789, 915
AcsI RAATTY 2 cut(s) 102, 399
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 3 cut(s) 723, 910, 946
AflIII ACRYGT 1 cut(s) 618
AgsI TTSAA 3 cut(s) 220, 485, 625
AjiI CACGTC 2 cut(s) 55, 317
AjnI CCWGG 3 cut(s) 92, 223, 909
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
AluBI AGCT 4 cut(s) 216, 366, 740, 899
AluI AGCT 4 cut(s) 216, 366, 740, 899
Alw26I GTCTC 1 cut(s) 553
AlwI GGATC 3 cut(s) 237, 357, 687
AoxI GGCC 7 cut(s) 371, 789, 901, 915, 921, 936, 948
ApeKI GCWGC 4 cut(s) 187, 423, 737, 830
ApoI RAATTY 2 cut(s) 102, 399
AspS9I GGNCC 6 cut(s) 371, 571, 728, 922, 937, 949
AvaII GGWCC 2 cut(s) 571, 728
BbsI GAAGAC 1 cut(s) 645
BbvI GCAGC 4 cut(s) 174, 435, 749, 817
BccI CCATC 4 cut(s) 276, 582, 725, 781
BceAI ACGGC 2 cut(s) 289, 963
BcgI CGANNNNNNTGC 2 cut(s) 375, 409
BciT130I CCWGG 3 cut(s) 94, 225, 911
BciVI GTATCC 1 cut(s) 724
BcoDI GTCTC 1 cut(s) 553
BfaI CTAG 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 452
BfuI GTATCC 1 cut(s) 724
BisI GCNGC 7 cut(s) 188, 424, 526, 738, 792, 831, 936
BlsI GCNGC 7 cut(s) 189, 425, 527, 739, 793, 832, 937
Bme1390I CCNGG 3 cut(s) 94, 225, 911
Bme18I GGWCC 2 cut(s) 571, 728
BmgBI CACGTC 2 cut(s) 55, 317
BmgT120I GGNCC 6 cut(s) 371, 571, 728, 922, 937, 949
BmiI GGNNCC 4 cut(s) 48, 532, 729, 939
BmrFI CCNGG 3 cut(s) 94, 225, 911
BmsI GCATC 3 cut(s) 285, 333, 477
BpiI GAAGAC 1 cut(s) 645
BpmI CTGGAG 1 cut(s) 893
Bpu10I CCTNAGC 1 cut(s) 367
BsaJI CCNNGG 4 cut(s) 92, 224, 410, 918
Bsc4I CCNNNNNNNGG 3 cut(s) 723, 910, 946
Bse3DI GCAATG 1 cut(s) 755
BseBI CCWGG 3 cut(s) 94, 225, 911
BseDI CCNNGG 4 cut(s) 92, 224, 410, 918
BseGI GGATG 2 cut(s) 792, 859
BseLI CCNNNNNNNGG 3 cut(s) 723, 910, 946
BseMI GCAATG 1 cut(s) 755
BseMII CTCAG 2 cut(s) 381, 564
BseRI GAGGAG 2 cut(s) 427, 862
BseXI GCAGC 4 cut(s) 174, 435, 749, 817
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 7 cut(s) 373, 791, 903, 917, 923, 938, 950
BslFI GGGAC 3 cut(s) 352, 678, 741
BslI CCNNNNNNNGG 3 cut(s) 723, 910, 946
BsmAI GTCTC 1 cut(s) 553
BsmFI GGGAC 3 cut(s) 352, 678, 741
BsnI GGCC 7 cut(s) 373, 791, 903, 917, 923, 938, 950
Bsp143I GATC 3 cut(s) 229, 349, 679
BspACI CCGC 5 cut(s) 426, 525, 528, 792, 935
BspANI GGCC 7 cut(s) 373, 791, 903, 917, 923, 938, 950
BspCNI CTCAG 2 cut(s) 380, 563
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 4 cut(s) 48, 532, 729, 939
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 3 cut(s) 237, 357, 687
BsrDI GCAATG 1 cut(s) 755
BssECI CCNNGG 4 cut(s) 92, 224, 410, 918
BssMI GATC 3 cut(s) 229, 349, 679
Bst2UI CCWGG 3 cut(s) 94, 225, 911
Bst4CI ACNGT 3 cut(s) 298, 635, 815
Bst6I CTCTTC 2 cut(s) 170, 411
BstC8I GCNNGC 4 cut(s) 375, 523, 901, 905
BstDEI CTNAG 2 cut(s) 367, 550
BstDSI CCRYGG 1 cut(s) 410
BstENI CCTNNNNNAGG 1 cut(s) 908
BstF5I GGATG 2 cut(s) 792, 859
BstFNI CGCG 1 cut(s) 525
BstKTI GATC 3 cut(s) 232, 352, 682
BstMAI GTCTC 1 cut(s) 553
BstMBI GATC 3 cut(s) 229, 349, 679
BstMWI GCNNNNNNNGC 4 cut(s) 402, 450, 609, 797
BstNI CCWGG 3 cut(s) 94, 225, 911
BstSCI CCNGG 3 cut(s) 92, 223, 909
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 4 cut(s) 174, 435, 749, 817
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 1 cut(s) 349
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 349
BsuI GTATCC 1 cut(s) 724
BsuRI GGCC 7 cut(s) 373, 791, 903, 917, 923, 938, 950
BtgI CCRYGG 1 cut(s) 410
BtrI CACGTC 2 cut(s) 55, 317
BtsCI GGATG 2 cut(s) 792, 859
BveI ACCTGC 1 cut(s) 452
Cac8I GCNNGC 4 cut(s) 375, 523, 901, 905
Cfr13I GGNCC 6 cut(s) 371, 571, 728, 922, 937, 949
CseI GACGC 1 cut(s) 749
Csp6I GTAC 1 cut(s) 537
CspCI CAANNNNNGTGG 2 cut(s) 873, 908
CviAII CATG 2 cut(s) 441, 844
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 2 cut(s) 367, 550
DpnI GATC 3 cut(s) 231, 351, 681
DpnII GATC 3 cut(s) 229, 349, 679
EaeI YGGCCR 2 cut(s) 789, 915
Eam1104I CTCTTC 2 cut(s) 170, 411
EarI CTCTTC 2 cut(s) 170, 411
EciI GGCGGA 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 571, 728
EcoNI CCTNNNNNAGG 1 cut(s) 908
EcoO109I RGGNCCY 1 cut(s) 728
EcoRII CCWGG 3 cut(s) 92, 223, 909
FaeI CATG 2 cut(s) 444, 847
FaiI YATR 9 cut(s) 258, 282, 442, 489, 722, 783, 828, 845, 881
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 3 cut(s) 352, 678, 741
FatI CATG 2 cut(s) 440, 843
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 7 cut(s) 188, 424, 526, 738, 792, 831, 936
FokI GGATG 2 cut(s) 799, 866
Fsp4HI GCNGC 7 cut(s) 188, 424, 526, 738, 792, 831, 936
FspBI CTAG 1 cut(s) 819
GluI GCNGC 7 cut(s) 188, 424, 526, 738, 792, 831, 936
GsuI CTGGAG 1 cut(s) 893
HaeIII GGCC 7 cut(s) 373, 791, 903, 917, 923, 938, 950
HgaI GACGC 1 cut(s) 749
Hin1II CATG 2 cut(s) 444, 847
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 2 cut(s) 97, 807
Hpy166II GTNNAC 4 cut(s) 320, 496, 750, 811
Hpy188I TCNGA 1 cut(s) 553
Hpy188III TCNNGA 2 cut(s) 625, 677
Hpy8I GTNNAC 4 cut(s) 320, 496, 750, 811
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 4 cut(s) 26, 37, 82, 856
HpyCH4III ACNGT 3 cut(s) 298, 635, 815
HpyCH4IV ACGT 3 cut(s) 54, 316, 618
HpyCH4V TGCA 7 cut(s) 405, 461, 515, 545, 657, 737, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 402, 450, 609, 797
HpyF3I CTNAG 2 cut(s) 367, 550
HpySE526I ACGT 3 cut(s) 54, 316, 618
Hsp92II CATG 2 cut(s) 444, 847
Kzo9I GATC 3 cut(s) 229, 349, 679
LmnI GCTCC 3 cut(s) 213, 896, 912
Lsp1109I GCAGC 4 cut(s) 174, 435, 749, 817
LweI GCATC 3 cut(s) 285, 333, 477
MaeI CTAG 1 cut(s) 819
MaeII ACGT 3 cut(s) 54, 316, 618
MaeIII GTNAC 1 cut(s) 766
MalI GATC 3 cut(s) 231, 351, 681
MboI GATC 3 cut(s) 229, 349, 679
MboII GAAGA 9 cut(s) 156, 159, 162, 165, 187, 344, 428, 650, 957
MflI RGATCY 1 cut(s) 349
MluCI AATT 3 cut(s) 102, 113, 399
MlyI GAGTC 1 cut(s) 816
MmeI TCCRAC 1 cut(s) 219
MseI TTAA 2 cut(s) 86, 116
MslI CAYNNNNRTG 1 cut(s) 842
MspA1I CMGCKG 3 cut(s) 426, 740, 935
MspR9I CCNGG 3 cut(s) 94, 225, 911
MvaI CCWGG 3 cut(s) 94, 225, 911
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 4 cut(s) 402, 450, 609, 797
NdeII GATC 3 cut(s) 229, 349, 679
NlaIII CATG 2 cut(s) 444, 847
NlaIV GGNNCC 4 cut(s) 48, 532, 729, 939
NmeAIII GCCGAG 1 cut(s) 943
NmuCI GTSAC 1 cut(s) 766
PaqCI CACCTGC 1 cut(s) 452
PfeI GAWTC 1 cut(s) 97
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 7 cut(s) 189, 425, 527, 739, 793, 832, 937
PleI GAGTC 1 cut(s) 815
PpsI GAGTC 1 cut(s) 815
PpuMI RGGWCCY 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 3 cut(s) 92, 223, 909
PspGI CCWGG 3 cut(s) 92, 223, 909
PspN4I GGNNCC 4 cut(s) 48, 532, 729, 939
PspPI GGNCC 6 cut(s) 371, 571, 728, 922, 937, 949
PspPPI RGGWCCY 1 cut(s) 728
PsuI RGATCY 1 cut(s) 349
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 2 cut(s) 86, 116
SatI GCNGC 7 cut(s) 188, 424, 526, 738, 792, 831, 936
Sau3AI GATC 3 cut(s) 229, 349, 679
Sau96I GGNCC 6 cut(s) 371, 571, 728, 922, 937, 949
SchI GAGTC 1 cut(s) 816
ScrFI CCNGG 3 cut(s) 94, 225, 911
SfaNI GCATC 3 cut(s) 285, 333, 477
SinI GGWCC 2 cut(s) 571, 728
SmiMI CAYNNNNRTG 1 cut(s) 842
Sse9I AATT 3 cut(s) 102, 113, 399
SsiI CCGC 5 cut(s) 426, 525, 528, 792, 935
SspMI CTAG 1 cut(s) 819
StyD4I CCNGG 3 cut(s) 92, 223, 909
TaaI ACNGT 3 cut(s) 298, 635, 815
TaiI ACGT 3 cut(s) 57, 319, 621
TaqI TCGA 3 cut(s) 385, 577, 873
TasI AATT 3 cut(s) 102, 113, 399
TauI GCSGC 3 cut(s) 528, 794, 938
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 2 cut(s) 86, 116
Tru9I TTAA 2 cut(s) 86, 116
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 4 cut(s) 187, 423, 737, 830
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 1 cut(s) 293
TspGWI ACGGA 1 cut(s) 9
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 2 cut(s) 571, 728
XagI CCTNNNNNAGG 1 cut(s) 908
XapI RAATTY 2 cut(s) 102, 399
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.