pycom14g03350

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011263
Physical Location & Seq
Reverse (-)
2694961 .. 2695602
642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03350.1

Sequence Viewer

Length: 642 bp
ATGCTGGATCTTCTCTTGGTTAGCTCAGGGCCAGCCCAACCTCAACAACCCTGCCAAATTTGCACTCCACGGGAAGAGGGCAGTGGTGTTCTCTCCTCATGCCTGAACTGCTTTCTTGCAGGTGGCATCTTGTATCGTTTTGAATATGCTGTCAACCCTGCTCTTTTTATTGCAAAAGCACGTGGCGGAACCTGTACCCTTGCAGTCTCAGACCCAGACGATGTTGTGGTCCATCGAGCAAGATACCTGTTTGAGAATGGCTTTGGTTGCTACAACGTGTTCAAGAACAACTGTGAAGACTTTGCTATTTACTGCAAAACGGGACTGCTTGTTCTGGATCAAGGAACAATCGGGCAAAGCGGCCAAGCAGTATCCATCATAGGGGGACCTCTTGCAGCTGTTTTGTCCACGCCATTGCGTCTTGTCACAACCAATGTTTATGGGATGGCCGCAACTGCTATTGGAGTCTACTGTGCTAGTAGGTATGCTGCTGACATTGGCATGAGGAGGGATGTAGTGAAGGTGTCGGTCGAGGATATGACGAGGAGAGTGGCAGCTGGCCTGCTCCAGGTGGCCGAGGCCCAAATCACAGCGGCCCCAATCACGGCCCTTGCAGGAGGTTCTTCGAACCTTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

214

Amino Acids

22.33

Weight (kDa)

5.8

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 19 - 110 7.1e-14 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06370
fragaria_vesca FvH4_6g20310 FvH4_6g20310 FvH4_6g20310
malus_domestica MD14G1039000.v1.1
prunus_persica Prupe.7G094600_v2.0.a1
pyrus_communis pycom14g03350
rosa_chinensis RchiOBHm_Chr3g0475931
rosa_laevigata RLG00000023799
rosa_multiflora Rmu_co8434183.1_g000001 Rmu_sc0000596.1_g000004 Rmu_sc0009168.1_g000002
rosa_roxburghii Rroxscaffold_6G00405720
rosa_rugosa Rorug03G0151900 Rorug03G0152000
rosa_samantha Rh3AG202900 Rh3BG232700 Rh3CG228200 Rh3DG228400
rosa_wichuraiana Rw3G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 468
AciI CCGC 4 cut(s) 186, 360, 450, 593
AclWI GGATC 2 cut(s) 15, 345
AcoI YGGCCR 3 cut(s) 361, 447, 573
AcsI RAATTY 1 cut(s) 57
AcvI CACGTG 1 cut(s) 182
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 3 cut(s) 381, 568, 604
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 143, 283
AjnI CCWGG 1 cut(s) 567
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 3 cut(s) 24, 398, 557
AluI AGCT 3 cut(s) 24, 398, 557
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 2 cut(s) 15, 345
AoxI GGCC 8 cut(s) 29, 361, 447, 559, 573, 579, 594, 606
ApeKI GCWGC 3 cut(s) 395, 488, 554
ApoI RAATTY 1 cut(s) 57
AspS9I GGNCC 6 cut(s) 29, 229, 386, 580, 595, 607
AsuII TTCGAA 1 cut(s) 626
AvaII GGWCC 2 cut(s) 229, 386
BbrPI CACGTG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 407, 475, 566
BccI CCATC 3 cut(s) 240, 383, 439
BceAI ACGGC 1 cut(s) 621
BciT130I CCWGG 1 cut(s) 569
BciVI GTATCC 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 110
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 6 cut(s) 361, 396, 450, 489, 555, 594
BlsI GCNGC 6 cut(s) 362, 397, 451, 490, 556, 595
Bme1390I CCNGG 1 cut(s) 569
Bme18I GGWCC 2 cut(s) 229, 386
BmgT120I GGNCC 6 cut(s) 29, 229, 386, 580, 595, 607
BmiI GGNNCC 3 cut(s) 190, 387, 597
BmrFI CCNGG 1 cut(s) 569
BmsI GCATC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 551
Bpu10I CCTNAGC 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 626
BsaAI YACGTR 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 68, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 381, 568, 604
Bse3DI GCAATG 1 cut(s) 413
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 2 cut(s) 68, 576
BseGI GGATG 2 cut(s) 450, 517
BseLI CCNNNNNNNGG 3 cut(s) 381, 568, 604
BseMI GCAATG 1 cut(s) 413
BseMII CTCAG 2 cut(s) 39, 222
BseRI GAGGAG 3 cut(s) 85, 520, 559
BseXI GCAGC 3 cut(s) 407, 475, 566
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 8 cut(s) 31, 363, 449, 561, 575, 581, 596, 608
BsiEI CGRYCG 1 cut(s) 531
BslFI GGGAC 2 cut(s) 336, 399
BslI CCNNNNNNNGG 3 cut(s) 381, 568, 604
BsmAI GTCTC 1 cut(s) 211
BsmFI GGGAC 2 cut(s) 336, 399
BsnI GGCC 8 cut(s) 31, 363, 449, 561, 575, 581, 596, 608
Bsp119I TTCGAA 1 cut(s) 626
Bsp143I GATC 2 cut(s) 7, 337
BspACI CCGC 4 cut(s) 186, 360, 450, 593
BspANI GGCC 8 cut(s) 31, 363, 449, 561, 575, 581, 596, 608
BspCNI CTCAG 2 cut(s) 38, 221
BspLI GGNNCC 3 cut(s) 190, 387, 597
BspMI ACCTGC 1 cut(s) 110
BspPI GGATC 2 cut(s) 15, 345
BspT104I TTCGAA 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 68, 576
BssMI GATC 2 cut(s) 7, 337
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 2 cut(s) 293, 473
Bst6I CTCTTC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 182
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 33, 559, 563
BstDEI CTNAG 2 cut(s) 25, 208
BstDSI CCRYGG 1 cut(s) 68
BstENI CCTNNNNNAGG 1 cut(s) 566
BstF5I GGATG 2 cut(s) 450, 517
BstKTI GATC 2 cut(s) 10, 340
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 7, 337
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 4 cut(s) 60, 108, 267, 455
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 407, 475, 566
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 8 cut(s) 31, 363, 449, 561, 575, 581, 596, 608
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 2 cut(s) 450, 517
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 88
BveI ACCTGC 1 cut(s) 110
Cac8I GCNNGC 3 cut(s) 33, 559, 563
Cfr13I GGNCC 6 cut(s) 29, 229, 386, 580, 595, 607
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 195
CviAII CATG 2 cut(s) 99, 502
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 2 cut(s) 25, 208
DpnI GATC 2 cut(s) 9, 339
DpnII GATC 2 cut(s) 7, 337
EaeI YGGCCR 3 cut(s) 361, 447, 573
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 229, 386
Eco72I CACGTG 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 567
FaeI CATG 2 cut(s) 102, 505
FaiI YATR 7 cut(s) 100, 147, 380, 441, 486, 503, 539
FaqI GGGAC 2 cut(s) 336, 399
FatI CATG 2 cut(s) 98, 501
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 361, 396, 450, 489, 555, 594
FokI GGATG 2 cut(s) 457, 524
Fsp4HI GCNGC 6 cut(s) 361, 396, 450, 489, 555, 594
FspBI CTAG 1 cut(s) 477
GluI GCNGC 6 cut(s) 361, 396, 450, 489, 555, 594
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 8 cut(s) 31, 363, 449, 561, 575, 581, 596, 608
HgaI GACGC 1 cut(s) 407
Hin1II CATG 2 cut(s) 102, 505
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 1 cut(s) 465
Hpy166II GTNNAC 3 cut(s) 154, 408, 469
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 2 cut(s) 283, 335
Hpy8I GTNNAC 3 cut(s) 154, 408, 469
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 2 cut(s) 293, 473
HpyCH4IV ACGT 2 cut(s) 181, 276
HpyCH4V TGCA 7 cut(s) 63, 119, 173, 203, 315, 395, 614
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 108, 267, 455
HpyF3I CTNAG 2 cut(s) 25, 208
HpySE526I ACGT 2 cut(s) 181, 276
Hsp92II CATG 2 cut(s) 102, 505
Kzo9I GATC 2 cut(s) 7, 337
LmnI GCTCC 1 cut(s) 570
Lsp1109I GCAGC 3 cut(s) 407, 475, 566
LweI GCATC 1 cut(s) 135
MaeI CTAG 1 cut(s) 477
MaeII ACGT 2 cut(s) 181, 276
MaeIII GTNAC 1 cut(s) 424
MalI GATC 2 cut(s) 9, 339
MboI GATC 2 cut(s) 7, 337
MboII GAAGA 3 cut(s) 86, 308, 615
MflI RGATCY 1 cut(s) 7
MluCI AATT 1 cut(s) 57
MlyI GAGTC 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 500
MspA1I CMGCKG 3 cut(s) 398, 557, 593
MspR9I CCNGG 1 cut(s) 569
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 4 cut(s) 60, 108, 267, 455
NdeII GATC 2 cut(s) 7, 337
NlaIII CATG 2 cut(s) 102, 505
NlaIV GGNNCC 3 cut(s) 190, 387, 597
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 424
NspV TTCGAA 1 cut(s) 626
PaqCI CACCTGC 1 cut(s) 110
PkrI GCNGC 6 cut(s) 362, 397, 451, 490, 556, 595
PleI GAGTC 1 cut(s) 473
PmaCI CACGTG 1 cut(s) 182
PmlI CACGTG 1 cut(s) 182
PpsI GAGTC 1 cut(s) 473
Ppu21I YACGTR 1 cut(s) 182
PpuMI RGGWCCY 1 cut(s) 386
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 567
PspCI CACGTG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 190, 387, 597
PspPI GGNCC 6 cut(s) 29, 229, 386, 580, 595, 607
PspPPI RGGWCCY 1 cut(s) 386
PsuI RGATCY 1 cut(s) 7
PvuII CAGCTG 2 cut(s) 398, 557
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 1 cut(s) 500
SatI GCNGC 6 cut(s) 361, 396, 450, 489, 555, 594
Sau3AI GATC 2 cut(s) 7, 337
Sau96I GGNCC 6 cut(s) 29, 229, 386, 580, 595, 607
SchI GAGTC 1 cut(s) 474
ScrFI CCNGG 1 cut(s) 569
SfaNI GCATC 1 cut(s) 135
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 2 cut(s) 229, 386
SmiMI CAYNNNNRTG 1 cut(s) 500
Sse9I AATT 1 cut(s) 57
SsiI CCGC 4 cut(s) 186, 360, 450, 593
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 567
TaaI ACNGT 2 cut(s) 293, 473
TaiI ACGT 2 cut(s) 184, 279
TaqI TCGA 3 cut(s) 235, 531, 626
TaqII GACCGA 1 cut(s) 517
TasI AATT 1 cut(s) 57
TauI GCSGC 3 cut(s) 363, 452, 596
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 3 cut(s) 395, 488, 554
Tsp45I GTSAC 1 cut(s) 424
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 229, 386
XagI CCTNNNNNAGG 1 cut(s) 566
XapI RAATTY 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.