MD14G1046200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012098
Physical Location & Seq
Forward (+)
4407225 .. 4409956
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1046200.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGAGGAATCGATTCGGAGCTTGTGCAAGGGCGTGGCGTCCTTCTGCAACCACCTCCAGAGCAGCTGCGACGCCCTCAAAACTTCCGTCGACCGCCGCCCCATCCCTCTGGATACGGCGTCGTCCACCTTCCTCCAATCACTCAACCGCCGCGTCTCCGCCGCCAGCACCCAACTCAACCTCCTCGAAACGATGTCGTTCGGCACCGTCTCCTTCGAGGAGCTCTTGGGACACTGCTCCGAGGTCTACAAGGACAACCAAACCCGCCTCCTCCACCTCCAACATCGTCTCTCCCCCTCCTCCGTCGCCGAACTTGAGATGGACGACGATGAAGATTTTACATCGACGCCGGTTGATGAGGAGGACGGATTCGATGCACCTGTCATTGATGAAGAAGAAGATTTATTGGATGAGTCTCTGAGTTTGAAGAAGCTTGGGCTTTCGGATGCATCTCTTGCATCTTTAGCAAATGATAAAAATGAGGACACTGATGTATCTATGCATGTGTTGAAATCCTTAAAGGGGTCATATGATCCTTCCACGAAGACCTCAGGAGCTTCGGGAGATAGATTGCTATCTGATTTTGGTGAAGTTGAGGATAAACCAAAGCTAGCTGAACTTCCTTTGCCTCTTGTAAAGGTGTCCCGGGATGATTACGAGAGCCTTCCTTCCTACATGAAAAGTTTGGCACCTTGGGAGGACCTGCTTGCCGCTGTCGAGAAGATTAACTCAAGCCTGAAACAGAAAGCGAGGGGGACTAATTTCTTCCATCAAGATGAAATTTCATCCTTGGGCTTAGGGCCGAAAGCGAGATCTTACTTGCTGCTGCTTGTACGCATGAATCGATTGGTTGTTGAGACCGTAAATGGTTTAATCTCATACCGAGTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

32.81

Weight (kDa)

4.71

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06590 AT5G06590
fragaria_vesca FvH4_6g21190 FvH4_6g21190
malus_domestica MD12G1047800.v1.1 MD14G1046200.v1.1
prunus_persica Prupe.7G088000_v2.0.a1
pyrus_communis pycom12g04100 pycom14g03870
rosa_chinensis RchiOBHm_Chr3g0477671
rosa_laevigata RLG00000023702
rosa_multiflora Rmu_sc0006187.1_g000003
rosa_roxburghii Rroxscaffold_6G00403770
rosa_rugosa Rorug03G0162600
rosa_samantha Rh3AG212600 Rh3BG246200 Rh3CG239900 Rh3DG239400
rosa_wichuraiana Rw3G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 711
AccB1I GGYRCC 2 cut(s) 203, 688
AccI GTMKAC 2 cut(s) 90, 246
AccII CGCG 1 cut(s) 153
AciI CCGC 8 cut(s) 94, 97, 148, 151, 159, 162, 265, 711
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 1 cut(s) 780
AcyI GRCGYC 4 cut(s) 38, 72, 119, 347
AfaI GTAC 1 cut(s) 834
AfiI CCNNNNNNNGG 1 cut(s) 522
AgsI TTSAA 2 cut(s) 427, 511
AluBI AGCT 7 cut(s) 21, 66, 223, 433, 557, 610, 614
AluI AGCT 7 cut(s) 21, 66, 223, 433, 557, 610, 614
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 5 cut(s) 160, 214, 293, 420, 851
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 645
AoxI GGCC 1 cut(s) 800
ApeKI GCWGC 4 cut(s) 63, 66, 823, 826
ApoI RAATTY 1 cut(s) 780
ArsI GACNNNNNNTTYG 2 cut(s) 72, 104
Asp700I GAANNNNTTC 1 cut(s) 12
AspS9I GGNCC 2 cut(s) 700, 800
AsuC2I CCSGG 2 cut(s) 646, 647
AsuHPI GGTGA 1 cut(s) 599
AsuNHI GCTAGC 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 645
AvaII GGWCC 1 cut(s) 700
AxyI CCTNAGG 1 cut(s) 550
BaeI ACNNNNGTAYC 2 cut(s) 477, 510
BanI GGYRCC 2 cut(s) 203, 688
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 551
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 53, 75, 810, 813
BccI CCATC 3 cut(s) 110, 313, 777
BceAI ACGGC 1 cut(s) 132
BciVI GTATCC 1 cut(s) 106
BcnI CCSGG 2 cut(s) 646, 647
BcoDI GTCTC 5 cut(s) 160, 214, 293, 420, 851
BfaI CTAG 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 711
BfuI GTATCC 1 cut(s) 106
BglII AGATCT 1 cut(s) 812
BisI GCNGC 8 cut(s) 64, 67, 97, 151, 162, 711, 824, 827
BlsI GCNGC 8 cut(s) 65, 68, 98, 152, 163, 712, 825, 828
Bme1390I CCNGG 2 cut(s) 646, 647
Bme18I GGWCC 1 cut(s) 700
BmeT110I CYCGRG 1 cut(s) 645
BmgT120I GGNCC 2 cut(s) 700, 800
BmiI GGNNCC 2 cut(s) 205, 690
BmrFI CCNGG 2 cut(s) 646, 647
BmsI GCATC 4 cut(s) 364, 436, 458, 467
BmtI GCTAGC 1 cut(s) 614
BpiI GAAGAC 1 cut(s) 551
BpmI CTGGAG 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 796
BpuEI CTTGAG 2 cut(s) 335, 715
BpuMI CCSGG 2 cut(s) 646, 647
Bsa29I ATCGAT 2 cut(s) 11, 844
BsaBI GATNNNNATC 1 cut(s) 574
BsaHI GRCGYC 4 cut(s) 38, 72, 119, 347
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 4 cut(s) 240, 645, 692, 789
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 1 cut(s) 522
Bse118I RCCGGY 1 cut(s) 349
Bse21I CCTNAGG 1 cut(s) 550
Bse8I GATNNNNATC 1 cut(s) 574
BseCI ATCGAT 2 cut(s) 11, 844
BseDI CCNNGG 4 cut(s) 240, 645, 692, 789
BseGI GGATG 5 cut(s) 102, 415, 451, 655, 785
BseJI GATNNNNATC 1 cut(s) 574
BseLI CCNNNNNNNGG 1 cut(s) 522
BseMII CTCAG 2 cut(s) 410, 564
BseRI GAGGAG 5 cut(s) 173, 233, 260, 289, 374
BseXI GCAGC 4 cut(s) 53, 75, 810, 813
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 802
BshNI GGYRCC 2 cut(s) 203, 688
BshVI ATCGAT 2 cut(s) 11, 844
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 645
BsiSI CCGG 2 cut(s) 350, 646
BslFI GGGAC 3 cut(s) 243, 628, 769
BslI CCNNNNNNNGG 1 cut(s) 522
BsmAI GTCTC 5 cut(s) 160, 214, 293, 420, 851
BsmBI CGTCTC 3 cut(s) 160, 214, 293
BsmFI GGGAC 3 cut(s) 243, 628, 769
BsnI GGCC 1 cut(s) 802
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 645
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 2 cut(s) 532, 812
BspACI CCGC 8 cut(s) 94, 97, 148, 151, 159, 162, 265, 711
BspANI GGCC 1 cut(s) 802
BspCNI CTCAG 2 cut(s) 411, 563
BspDI ATCGAT 2 cut(s) 11, 844
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 205, 690
BspMI ACCTGC 1 cut(s) 711
BspOI GCTAGC 1 cut(s) 614
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 203, 688
BspTNI GGTCTC 1 cut(s) 851
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 4 cut(s) 240, 645, 692, 789
BssMI GATC 2 cut(s) 532, 812
BssNI GRCGYC 4 cut(s) 38, 72, 119, 347
BssT1I CCWWGG 2 cut(s) 692, 789
Bst4CI ACNGT 2 cut(s) 208, 862
BstACI GRCGYC 4 cut(s) 38, 72, 119, 347
BstAPI GCANNNNNTGC 1 cut(s) 455
BstC8I GCNNGC 3 cut(s) 166, 612, 708
BstDEI CTNAG 3 cut(s) 419, 550, 796
BstF5I GGATG 5 cut(s) 102, 415, 451, 655, 785
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 2 cut(s) 535, 815
BstMAI GTCTC 5 cut(s) 160, 214, 293, 420, 851
BstMBI GATC 2 cut(s) 532, 812
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 2 cut(s) 455, 464
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 644, 645
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 4 cut(s) 53, 75, 810, 813
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 812
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 1 cut(s) 812
Bsu15I ATCGAT 2 cut(s) 11, 844
Bsu36I CCTNAGG 1 cut(s) 550
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 802
BsuTUI ATCGAT 2 cut(s) 11, 844
BtsCI GGATG 5 cut(s) 102, 415, 451, 655, 785
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 232, 486
BveI ACCTGC 1 cut(s) 711
Cac8I GCNNGC 3 cut(s) 166, 612, 708
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 700, 800
Cfr9I CCCGGG 1 cut(s) 645
ClaI ATCGAT 2 cut(s) 11, 844
CseI GACGC 5 cut(s) 27, 80, 108, 142, 355
Csp6I GTAC 1 cut(s) 833
CviAII CATG 3 cut(s) 503, 676, 838
CviQI GTAC 1 cut(s) 833
DdeI CTNAG 3 cut(s) 419, 550, 796
DpnI GATC 2 cut(s) 534, 814
DpnII GATC 2 cut(s) 532, 812
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 692, 789
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 851
Eco47I GGWCC 1 cut(s) 700
Eco53kI GAGCTC 1 cut(s) 223
Eco81I CCTNAGG 1 cut(s) 550
Eco88I CYCGRG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 700
EcoT14I CCWWGG 2 cut(s) 692, 789
EcoT22I ATGCAT 2 cut(s) 451, 504
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 2 cut(s) 692, 789
Esp3I CGTCTC 3 cut(s) 160, 214, 293
FaeI CATG 3 cut(s) 506, 679, 841
FaiI YATR 7 cut(s) 500, 504, 529, 531, 677, 839, 880
FaqI GGGAC 3 cut(s) 243, 628, 769
FatI CATG 3 cut(s) 502, 675, 837
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 529
FblI GTMKAC 2 cut(s) 90, 246
Fnu4HI GCNGC 8 cut(s) 64, 67, 97, 151, 162, 711, 824, 827
FokI GGATG 5 cut(s) 89, 422, 458, 662, 772
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 8 cut(s) 64, 67, 97, 151, 162, 711, 824, 827
FspBI CTAG 1 cut(s) 611
GluI GCNGC 8 cut(s) 64, 67, 97, 151, 162, 711, 824, 827
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 1 cut(s) 802
HapII CCGG 2 cut(s) 350, 646
HgaI GACGC 5 cut(s) 27, 80, 108, 142, 355
Hin1I GRCGYC 4 cut(s) 38, 72, 119, 347
Hin1II CATG 3 cut(s) 506, 679, 841
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 5 cut(s) 8, 13, 369, 413, 841
HpaII CCGG 2 cut(s) 350, 646
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 3 cut(s) 91, 126, 247
Hpy188I TCNGA 5 cut(s) 18, 241, 420, 445, 580
Hpy188III TCNNGA 6 cut(s) 58, 110, 552, 561, 718, 773
Hpy8I GTNNAC 3 cut(s) 91, 126, 247
Hpy99I CGWCG 6 cut(s) 74, 92, 124, 308, 329, 349
HpyAV CCTTC 6 cut(s) 52, 139, 223, 546, 674, 678
HpyCH4III ACNGT 2 cut(s) 208, 862
HpyCH4V TGCA 6 cut(s) 27, 48, 377, 449, 458, 502
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 464
HpyF3I CTNAG 3 cut(s) 419, 550, 796
Hsp92I GRCGYC 4 cut(s) 38, 72, 119, 347
Hsp92II CATG 3 cut(s) 506, 679, 841
Kzo9I GATC 2 cut(s) 532, 812
LmnI GCTCC 4 cut(s) 18, 220, 242, 554
LpnPI CCDG 9 cut(s) 71, 95, 178, 363, 393, 537, 659, 716, 749
Lsp1109I GCAGC 4 cut(s) 53, 75, 810, 813
LweI GCATC 4 cut(s) 364, 436, 458, 467
MaeI CTAG 1 cut(s) 611
MalI GATC 2 cut(s) 534, 814
MboI GATC 2 cut(s) 532, 812
MboII GAAGA 8 cut(s) 344, 404, 407, 410, 439, 556, 733, 757
MflI RGATCY 1 cut(s) 812
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 2 cut(s) 760, 780
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 304
Mph1103I ATGCAT 2 cut(s) 451, 504
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 3 cut(s) 518, 726, 872
MslI CAYNNNNRTG 1 cut(s) 774
MspA1I CMGCKG 2 cut(s) 66, 713
MspI CCGG 2 cut(s) 350, 646
MspR9I CCNGG 2 cut(s) 646, 647
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 455, 464
NciI CCSGG 2 cut(s) 646, 647
NdeI CATATG 1 cut(s) 529
NdeII GATC 2 cut(s) 532, 812
NheI GCTAGC 1 cut(s) 610
NlaIII CATG 3 cut(s) 506, 679, 841
NlaIV GGNNCC 2 cut(s) 205, 690
NsiI ATGCAT 2 cut(s) 451, 504
NspI RCATGY 1 cut(s) 506
PcsI WCGNNNNNNNCGW 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 4 cut(s) 8, 13, 369, 841
PkrI GCNGC 8 cut(s) 65, 68, 98, 152, 163, 712, 825, 828
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 700
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 700
PspN4I GGNNCC 2 cut(s) 205, 690
PspPI GGNCC 2 cut(s) 700, 800
PspPPI RGGWCCY 1 cut(s) 700
PsuI RGATCY 1 cut(s) 812
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 1 cut(s) 834
RsaNI GTAC 1 cut(s) 833
RseI CAYNNNNRTG 1 cut(s) 774
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 3 cut(s) 518, 726, 872
SatI GCNGC 8 cut(s) 64, 67, 97, 151, 162, 711, 824, 827
Sau3AI GATC 2 cut(s) 532, 812
Sau96I GGNCC 2 cut(s) 700, 800
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 2 cut(s) 646, 647
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 4 cut(s) 364, 436, 458, 467
SinI GGWCC 1 cut(s) 700
SmaI CCCGGG 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 2 cut(s) 314, 730
SmoI CTYRAG 2 cut(s) 314, 730
Sse9I AATT 2 cut(s) 760, 780
SsiI CCGC 8 cut(s) 94, 97, 148, 151, 159, 162, 265, 711
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 644, 645
StyI CCWWGG 2 cut(s) 692, 789
TaaI ACNGT 2 cut(s) 208, 862
TaqI TCGA 8 cut(s) 11, 90, 186, 216, 344, 372, 717, 844
TasI AATT 2 cut(s) 760, 780
TauI GCSGC 4 cut(s) 99, 153, 164, 713
TfiI GAWTC 4 cut(s) 8, 13, 369, 841
Tru1I TTAA 3 cut(s) 518, 726, 872
Tru9I TTAA 3 cut(s) 518, 726, 872
TscAI CASTG 2 cut(s) 239, 493
TseI GCWGC 4 cut(s) 63, 66, 823, 826
TspDTI ATGAA 6 cut(s) 345, 405, 692, 774, 792, 854
TspGWI ACGGA 3 cut(s) 76, 292, 381
TspMI CCCGGG 1 cut(s) 645
TspRI CASTG 2 cut(s) 239, 493
VpaK11BI GGWCC 1 cut(s) 700
XapI RAATTY 1 cut(s) 780
XceI RCATGY 1 cut(s) 506
XmaI CCCGGG 1 cut(s) 645
XmiI GTMKAC 2 cut(s) 90, 246
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 1 cut(s) 611
Zsp2I ATGCAT 2 cut(s) 451, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.