pycom12g04100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012098
Physical Location & Seq
Forward (+)
4049332 .. 4051439
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04100.3

Sequence Viewer

Length: 645 bp
ATGGAGGAATCGATTCGGAGCTTGTGCAAGGGCGTAGCGTCCTTCTGCAACCACCTCCACAGCAGCTGCGACGCCCTCAAAGCTTCTGTCGACCGCCGCCCCATCCCTCTCGATACGGCGTTGTCCAACTTCCTCCAATCCCTCAAACGCCGCGTCTCCGCCGCCAGCACCCAACTCAACCTCCTTGAAACGATGTCGTTCGGCACCGTCCCCTTCGAGGAGCTCTTGGGACACTGCTGCGAGGTCCAAGGAAAACCAAACCCGCCTCCTCCACCTCCAACGTCATCTCGCCCCCTCCACCGTCGCCGATTGGACGAGTCTTTGAGTTTGAAGAAGCTCGGGCTTTCGGATGCGTCTCTTGCTACTTTAGCATCTCAAGGGGAAGTTGAGGATAAACCAAAGCTAGCTGAAGTTCCTTTGCCTCTTGTAAAGGCAGGTATCCCGGATGACTACGAGAGCCTTCCTTCCTACATGAAAAGTCTAGGACCTTGGGAGGACTTGCTTGCCGCTGTCGAGAAGGTTAATTCAAGCCTGAAACAGAAAGCAGGGGGGAATAATTTCTTCCATCAAGATGAAATTTCGTCCTTGGGCTTAGATCATATCCAATGGAGTTTCATAAAATACGAGTCTTACGAATCCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

215

Amino Acids

23.52

Weight (kDa)

5.88

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06590 AT5G06590
fragaria_vesca FvH4_6g21190 FvH4_6g21190
malus_domestica MD12G1047800.v1.1 MD14G1046200.v1.1
prunus_persica Prupe.7G088000_v2.0.a1
pyrus_communis pycom12g04100 pycom14g03870
rosa_chinensis RchiOBHm_Chr3g0477671
rosa_laevigata RLG00000023702
rosa_multiflora Rmu_sc0006187.1_g000003
rosa_roxburghii Rroxscaffold_6G00403770
rosa_rugosa Rorug03G0162600
rosa_samantha Rh3AG212600 Rh3BG246200 Rh3CG239900 Rh3DG239400
rosa_wichuraiana Rw3G019250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 153
AciI CCGC 7 cut(s) 94, 97, 151, 159, 162, 263, 507
AcsI RAATTY 1 cut(s) 576
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 3 cut(s) 188, 331, 528
AluBI AGCT 7 cut(s) 21, 66, 83, 223, 337, 403, 407
AluI AGCT 7 cut(s) 21, 66, 83, 223, 337, 403, 407
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 160, 360
AlwNI CAGNNNCTG 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 338
ApeKI GCWGC 3 cut(s) 63, 66, 237
ApoI RAATTY 1 cut(s) 576
ArsI GACNNNNNNTTYG 4 cut(s) 72, 104, 138, 170
Asp700I GAANNNNTTC 2 cut(s) 12, 557
AspS9I GGNCC 2 cut(s) 244, 485
AsuC2I CCSGG 1 cut(s) 443
AsuNHI GCTAGC 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 244, 485
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 53, 75, 224
BccI CCATC 2 cut(s) 110, 573
BceAI ACGGC 1 cut(s) 132
BciVI GTATCC 1 cut(s) 449
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 2 cut(s) 160, 360
BfaI CTAG 2 cut(s) 404, 482
BfuAI ACCTGC 1 cut(s) 425
BfuI GTATCC 1 cut(s) 449
BisI GCNGC 7 cut(s) 64, 67, 97, 151, 162, 238, 507
BlsI GCNGC 7 cut(s) 65, 68, 98, 152, 163, 239, 508
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 2 cut(s) 244, 485
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 244, 485
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 2 cut(s) 340, 380
BmtI GCTAGC 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 247, 488, 585
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 247, 488, 585
BseGI GGATG 3 cut(s) 102, 355, 451
BseLI CCNNNNNNNGG 1 cut(s) 217
BseRI GAGGAG 2 cut(s) 233, 258
BseXI GCAGC 3 cut(s) 53, 75, 224
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 94
BshNI GGYRCC 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 443
BslFI GGGAC 2 cut(s) 194, 243
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 160, 360
BsmBI CGTCTC 2 cut(s) 160, 360
BsmFI GGGAC 2 cut(s) 194, 243
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 1 cut(s) 595
BspACI CCGC 7 cut(s) 94, 97, 151, 159, 162, 263, 507
BspDI ATCGAT 1 cut(s) 11
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 425
BspOI GCTAGC 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 247, 488, 585
BssMI GATC 1 cut(s) 595
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 3 cut(s) 247, 488, 585
Bst4CI ACNGT 2 cut(s) 208, 302
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 3 cut(s) 166, 405, 504
BstDEI CTNAG 1 cut(s) 592
BstF5I GGATG 3 cut(s) 102, 355, 451
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 1 cut(s) 598
BstMAI GTCTC 2 cut(s) 160, 360
BstMBI GATC 1 cut(s) 595
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 3 cut(s) 80, 359, 368
BstSCI CCNGG 1 cut(s) 441
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 3 cut(s) 53, 75, 224
Bsu15I ATCGAT 1 cut(s) 11
BsuI GTATCC 1 cut(s) 449
BsuTUI ATCGAT 1 cut(s) 11
BtsCI GGATG 3 cut(s) 102, 355, 451
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
BveI ACCTGC 1 cut(s) 425
Cac8I GCNNGC 3 cut(s) 166, 405, 504
CaiI CAGNNNCTG 1 cut(s) 66
Cfr13I GGNCC 2 cut(s) 244, 485
ClaI ATCGAT 1 cut(s) 11
CseI GACGC 4 cut(s) 27, 80, 142, 342
CviAII CATG 1 cut(s) 472
DdeI CTNAG 1 cut(s) 592
DpnI GATC 1 cut(s) 597
DpnII GATC 1 cut(s) 595
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 3 cut(s) 247, 488, 585
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 244, 485
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 485
EcoT14I CCWWGG 3 cut(s) 247, 488, 585
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 3 cut(s) 247, 488, 585
Esp3I CGTCTC 2 cut(s) 160, 360
FaeI CATG 1 cut(s) 475
FaiI YATR 3 cut(s) 473, 600, 617
FaqI GGGAC 2 cut(s) 194, 243
FatI CATG 1 cut(s) 471
FauI CCCGC 1 cut(s) 270
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 7 cut(s) 64, 67, 97, 151, 162, 238, 507
FokI GGATG 3 cut(s) 89, 362, 458
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 7 cut(s) 64, 67, 97, 151, 162, 238, 507
FspBI CTAG 2 cut(s) 404, 482
GluI GCNGC 7 cut(s) 64, 67, 97, 151, 162, 238, 507
HapII CCGG 1 cut(s) 443
HgaI GACGC 4 cut(s) 27, 80, 142, 342
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 475
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 5 cut(s) 8, 13, 317, 626, 635
HpaII CCGG 1 cut(s) 443
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 18, 349
Hpy188III TCNNGA 3 cut(s) 110, 514, 569
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 2 cut(s) 74, 306
HpyAV CCTTC 5 cut(s) 52, 223, 470, 474, 511
HpyCH4III ACNGT 2 cut(s) 208, 302
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 2 cut(s) 27, 48
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 359, 368
HpyF3I CTNAG 1 cut(s) 592
HpySE526I ACGT 1 cut(s) 281
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 475
Kzo9I GATC 1 cut(s) 595
LmnI GCTCC 2 cut(s) 18, 220
LpnPI CCDG 5 cut(s) 178, 420, 456, 531, 545
Lsp1109I GCAGC 3 cut(s) 53, 75, 224
LweI GCATC 2 cut(s) 340, 380
MaeI CTAG 2 cut(s) 404, 482
MaeII ACGT 1 cut(s) 281
MalI GATC 1 cut(s) 597
MboI GATC 1 cut(s) 595
MboII GAAGA 2 cut(s) 343, 553
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 3 cut(s) 523, 556, 576
MlyI GAGTC 2 cut(s) 326, 635
MmeI TCCRAC 2 cut(s) 150, 302
MroXI GAANNNNTTC 2 cut(s) 12, 557
MseI TTAA 1 cut(s) 522
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 2 cut(s) 66, 509
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 1 cut(s) 443
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 80, 359, 368
NciI CCSGG 1 cut(s) 443
NdeII GATC 1 cut(s) 595
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 1 cut(s) 475
NlaIV GGNNCC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 12, 557
PfeI GAWTC 3 cut(s) 8, 13, 635
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 7 cut(s) 65, 68, 98, 152, 163, 239, 508
PleI GAGTC 2 cut(s) 325, 634
PpsI GAGTC 2 cut(s) 325, 634
PpuMI RGGWCCY 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 485
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 2 cut(s) 244, 485
PspPPI RGGWCCY 1 cut(s) 485
PstNI CAGNNNCTG 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 570
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 7 cut(s) 64, 67, 97, 151, 162, 238, 507
Sau3AI GATC 1 cut(s) 595
Sau96I GGNCC 2 cut(s) 244, 485
SchI GAGTC 2 cut(s) 326, 635
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 2 cut(s) 340, 380
SinI GGWCC 2 cut(s) 244, 485
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 375
SmoI CTYRAG 1 cut(s) 375
Sse9I AATT 3 cut(s) 523, 556, 576
SsiI CCGC 7 cut(s) 94, 97, 151, 159, 162, 263, 507
SspMI CTAG 2 cut(s) 404, 482
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 441
StyI CCWWGG 3 cut(s) 247, 488, 585
TaaI ACNGT 2 cut(s) 208, 302
TaiI ACGT 1 cut(s) 284
TaqI TCGA 5 cut(s) 11, 90, 111, 216, 513
TasI AATT 3 cut(s) 523, 556, 576
TauI GCSGC 4 cut(s) 99, 153, 164, 509
TfiI GAWTC 3 cut(s) 8, 13, 635
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 1 cut(s) 239
TseI GCWGC 3 cut(s) 63, 66, 237
TspDTI ATGAA 3 cut(s) 488, 588, 604
TspRI CASTG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 244, 485
XapI RAATTY 1 cut(s) 576
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 2 cut(s) 12, 557
XspI CTAG 2 cut(s) 404, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.