Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004381

Physical Location & Seq

Genomic Coordinates Forward (+)

5744712 .. 5746648

Gene Structure 1937 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1055900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1287 bp

ATGACCCAGACGTCTGATGCAATGAACCTTGACCTGAATCTGGGTCCTGGTCCCGATGCTGGCTCAATGTCAAATGAACCTGTAAATTTGGATGCTTGGACTGAAGCGCCTATCCGCAGAATTGCGGAAGCTATGAGGAGGGCCAGGCAGAGGAGATGGCCGCTACCTGAAAGGCGGAATATTTCCACGCTACCTGAAAGGCGGAATATTTCCATGCTACCTGAAAGGCGGAATATTTCCATGGAGTTGAATGAATTGATGGTCAACTCTGGTAACTCAAGTACATTACAACCCGGTGAGGGTAGCCTTACAGCAGAAGAAAGAACAAGTGACGCACCCAAGACATGTGATAATAACAATGGGATTTTGGAAAATGAGAATTCTGAGAATAAGGATGATGTTGAAAAGGGCGGTGGCAGTGATGGGAGCTTTTTCGATTGTAATATTTGTTTGGACTTGGCTAGGGACCCTGTTGTGACTTGTTGTGGTCATTTGTTTTGTTGGCCATGCATTTACCGTTGGTTGCATGTGCAAGATGCAAAGGAATGCCCTGTTTGTAAGGGAGAGGTAACAATGAAGAATGTCACCCCAATCTATGGTCGTGGAAACAACGTGCGTGAGCAAGATGACGACTCAAATCTTAAGATCCCTCTTAGGCCTCATGCACGTCGGGTTGAAAGTTTTAGACAAACCATTCAGAGGAGTGCATTGAGTTTTCCGGTAGAGGAGATGATTCGCCGTCTTGGTAATAGATTTGATTTGACTCGGGATGTTATTCATCCATCAGAGCCTGAGAATACCCGGGAAGCAGCAGAAAGAACTAATTTGTTAAATAGGATTCTGACATCTCGAGGACTGCGCAGAGAGCAAAATCCAGTGGTACTTGCAGATGATGTTGTGGATTTAACGCAAAGCAACGTGACCGCTGTTGAAACAAGGGAAAACCGCCAACTTCAGTCCTTACTACTTAGAAGGACACAATCGCACAGAGCAACACTATCTTCTCTCTCGTCTGCATTGAGTTCTGCTGAAAGGTTAGTTGAGGCATATTATCGTAACCGTAATAGAAATCAAGAACAACAGCAGCCTGCACCAGTTGATGATAGAGATTCTTTCTCAAGTATTGCAGCTGTTATAAATTCAGAGAGCCAACTGGACACAGCTGTGGAAATTGATTCCATGGTTTCCCTTTCAACTTCATCTTCCAGAAGAAGAAATGATTCGTCAAGAGTTTCGGACATGGACAGTGGAGATTCTAGTGCCCACAGAAGAAGAAGACTTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006515 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0051788 GO:0070647 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0098796 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

429

Amino Acids

48.32

Weight (kDa)

5.86

Isoelectric Point (pI)

73.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	146 - 187	4e-07	Ring finger domain
zf-RING_5	PF14634	146 - 187	6.5e-07	zinc-RING finger domain
zf-C3HC4_2	PF13923	147 - 186	1.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	147 - 186	1.9e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	147 - 184	1.8e-07	RING-type zinc-finger
zf-C3HC4_3	PF13920	147 - 191	1.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4	PF15227	147 - 186	1.7e-06	zinc finger of C3HC4-type, RING

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004381)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58030 AT3G58030 AT3G58030 AT3G58030
fragaria_vesca	FvH4_6g22060
malus_domestica	MD02G1306100.v1.1 MD12G1055900.v1.1 MD14G1055900.v1.1 MD14G1056100.v1.1
prunus_persica	Prupe.7G079200_v2.0.a1
pyrus_communis	pycom12g04880
rosa_chinensis	RchiOBHm_Chr3g0479381 RchiOBHm_Chr3g0479391
rosa_laevigata	RLG00000023579
rosa_multiflora	Rmu_co8192992.1_g000001 Rmu_sc0000107.1_g000001 Rmu_sc0006208.1_g000003 Rmu_sc0006208.1_g000006
rosa_roxburghii	Rroxscaffold_6G00402310 Rroxscaffold_6G00402330 Rroxscaffold_6G00402350
rosa_rugosa	Rorug03G0174800 Rorug03G0174900
rosa_samantha	Rh3AG226000 Rh3AG226100 Rh3BG258700 Rh3BG258800 Rh3CG254800 Rh3CG255000 Rh3DG250300 Rh3DG250500
rosa_wichuraiana	Rw3G020180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1136
AasI	GACNNNNNNGTC	1 cut(s)	10
AatII	GACGTC	1 cut(s)	14
Acc16I	TGCGCA	1 cut(s)	860
AccB7I	CCANNNNNTGG	1 cut(s)	596
AciI	CCGC	9 cut(s)	115, 125, 161, 175, 202, 229, 411, 924, 946
AclWI	GGATC	1 cut(s)	640
AcoI	YGGCCR	2 cut(s)	158, 503
AcsI	RAATTY	3 cut(s)	85, 379, 1138
AcuI	CTGAAG	2 cut(s)	123, 938
AcyI	GRCGYC	1 cut(s)	11
AfaI	GTAC	2 cut(s)	283, 882
AfiI	CCNNNNNNNGG	6 cut(s)	40, 59, 150, 299, 596, 699
AflII	CTTAAG	1 cut(s)	641
AflIII	ACRYGT	1 cut(s)	344
AgsI	TTSAA	5 cut(s)	250, 404, 677, 932, 1194
AjiI	CACGTC	1 cut(s)	668
AjnI	CCWGG	2 cut(s)	46, 143
AleI	CACNNNNGTG	1 cut(s)	1163
AluBI	AGCT	4 cut(s)	131, 429, 1130, 1163
AluI	AGCT	4 cut(s)	131, 429, 1130, 1163
AlwI	GGATC	1 cut(s)	640
AlwNI	CAGNNNCTG	1 cut(s)	791
Ama87I	CYCGRG	3 cut(s)	765, 801, 849
AoxI	GGCC	4 cut(s)	141, 158, 503, 656
ApeKI	GCWGC	3 cut(s)	809, 1084, 1127
ApoI	RAATTY	3 cut(s)	85, 379, 1138
Asp700I	GAANNNNTTC	1 cut(s)	1219
AspLEI	GCGC	2 cut(s)	109, 861
AspS9I	GGNCC	4 cut(s)	44, 50, 141, 466
AsuC2I	CCSGG	3 cut(s)	294, 802, 803
AsuHPI	GGTGA	2 cut(s)	308, 577
AvaI	CYCGRG	3 cut(s)	765, 801, 849
AvaII	GGWCC	3 cut(s)	44, 50, 466
BaeGI	GKGCMC	1 cut(s)	1264
BalI	TGGCCA	1 cut(s)	505
BbsI	GAAGAC	1 cut(s)	1282
BbvI	GCAGC	3 cut(s)	821, 1096, 1139
BccI	CCATC	4 cut(s)	150, 253, 416, 790
BceAI	ACGGC	1 cut(s)	723
BciT130I	CCWGG	2 cut(s)	48, 145
BcnI	CCSGG	3 cut(s)	294, 802, 803
BfaI	CTAG	2 cut(s)	462, 1257
BfoI	RGCGCY	1 cut(s)	110
BfrI	CTTAAG	1 cut(s)	641
BisI	GCNGC	4 cut(s)	161, 810, 1085, 1128
BlsI	GCNGC	4 cut(s)	162, 811, 1086, 1129
Bme1390I	CCNGG	5 cut(s)	48, 145, 294, 802, 803
Bme18I	GGWCC	3 cut(s)	44, 50, 466
BmeT110I	CYCGRG	3 cut(s)	765, 801, 849
BmgBI	CACGTC	1 cut(s)	668
BmgT120I	GGNCC	4 cut(s)	44, 50, 141, 466
BmiI	GGNNCC	4 cut(s)	45, 52, 467, 468
BmrFI	CCNGG	5 cut(s)	48, 145, 294, 802, 803
BmsI	GCATC	4 cut(s)	7, 46, 82, 526
BpiI	GAAGAC	1 cut(s)	1282
BpuEI	CTTGAG	2 cut(s)	262, 1102
BpuMI	CCSGG	3 cut(s)	294, 802, 803
BsaHI	GRCGYC	1 cut(s)	11
BsaJI	CCNNGG	3 cut(s)	240, 801, 1179
BsaWI	WCCGGW	1 cut(s)	718
Bsc4I	CCNNNNNNNGG	6 cut(s)	40, 59, 150, 299, 596, 699
Bse1I	ACTGG	3 cut(s)	875, 1094, 1158
Bse3DI	GCAATG	1 cut(s)	27
BseBI	CCWGG	2 cut(s)	48, 145
BseDI	CCNNGG	3 cut(s)	240, 801, 1179
BseGI	GGATG	4 cut(s)	97, 400, 775, 778
BseLI	CCNNNNNNNGG	6 cut(s)	40, 59, 150, 299, 596, 699
BseMI	GCAATG	1 cut(s)	27
BseMII	CTCAG	2 cut(s)	375, 783
BseNI	ACTGG	3 cut(s)	875, 1094, 1158
BseRI	GAGGAG	4 cut(s)	151, 166, 715, 740
BseSI	GKGCMC	1 cut(s)	1264
BseXI	GCAGC	3 cut(s)	821, 1096, 1139
BsgI	GTGCAG	1 cut(s)	1074
BshFI	GGCC	4 cut(s)	143, 160, 505, 658
BsiHKCI	CYCGRG	3 cut(s)	765, 801, 849
BsiSI	CCGG	3 cut(s)	294, 719, 802
BslFI	GGGAC	2 cut(s)	36, 479
BslI	CCNNNNNNNGG	6 cut(s)	40, 59, 150, 299, 596, 699
BsmFI	GGGAC	2 cut(s)	36, 479
BsmI	GAATGC	1 cut(s)	551
BsnI	GGCC	4 cut(s)	143, 160, 505, 658
BsoBI	CYCGRG	3 cut(s)	765, 801, 849
Bsp1286I	GDGCHC	1 cut(s)	1264
Bsp143I	GATC	1 cut(s)	645
Bsp19I	CCATGG	2 cut(s)	240, 1179
BspACI	CCGC	9 cut(s)	115, 125, 161, 175, 202, 229, 411, 924, 946
BspANI	GGCC	4 cut(s)	143, 160, 505, 658
BspCNI	CTCAG	2 cut(s)	376, 784
BspLI	GGNNCC	4 cut(s)	45, 52, 467, 468
BspPI	GGATC	1 cut(s)	640
BspTI	CTTAAG	1 cut(s)	641
BsrDI	GCAATG	1 cut(s)	27
BsrI	ACTGG	3 cut(s)	875, 1094, 1158
BssECI	CCNNGG	3 cut(s)	240, 801, 1179
BssMI	GATC	1 cut(s)	645
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	2 cut(s)	240, 1179
Bst2UI	CCWGG	2 cut(s)	48, 145
Bst4CI	ACNGT	3 cut(s)	518, 1061, 1247
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	1 cut(s)	641
BstC8I	GCNNGC	2 cut(s)	61, 1089
BstDEI	CTNAG	4 cut(s)	384, 653, 792, 968
BstDSI	CCRYGG	2 cut(s)	240, 1179
BstF5I	GGATG	4 cut(s)	97, 400, 775, 778
BstH2I	RGCGCY	1 cut(s)	110
BstHHI	GCGC	2 cut(s)	109, 861
BstKTI	GATC	1 cut(s)	648
BstMBI	GATC	1 cut(s)	645
BstMWI	GCNNNNNNNGC	1 cut(s)	865
BstNI	CCWGG	2 cut(s)	48, 145
BstNSI	RCATGY	2 cut(s)	348, 530
BstSCI	CCNGG	5 cut(s)	46, 143, 292, 800, 801
BstSLI	GKGCMC	1 cut(s)	1264
BstV1I	GCAGC	3 cut(s)	821, 1096, 1139
BstV2I	GAAGAC	1 cut(s)	1282
BstX2I	RGATCY	1 cut(s)	645
BstYI	RGATCY	1 cut(s)	645
BsuRI	GGCC	4 cut(s)	143, 160, 505, 658
BtgI	CCRYGG	2 cut(s)	240, 1179
BtrI	CACGTC	1 cut(s)	668
BtsCI	GGATG	4 cut(s)	97, 400, 775, 778
BtsI	GCAGTG	1 cut(s)	424
BtsIMutI	CAGTG	3 cut(s)	424, 882, 1252
Cac8I	GCNNGC	2 cut(s)	61, 1089
CaiI	CAGNNNCTG	1 cut(s)	791
CfoI	GCGC	2 cut(s)	109, 861
Cfr13I	GGNCC	4 cut(s)	44, 50, 141, 466
Cfr9I	CCCGGG	1 cut(s)	801
CseI	GACGC	1 cut(s)	341
Csp6I	GTAC	2 cut(s)	282, 881
CviAII	CATG	8 cut(s)	214, 241, 345, 507, 527, 662, 1180, 1240
CviQI	GTAC	2 cut(s)	282, 881
DdeI	CTNAG	4 cut(s)	384, 653, 792, 968
DpnI	GATC	1 cut(s)	647
DpnII	GATC	1 cut(s)	645
DrdI	GACNNNNNNGTC	1 cut(s)	10
DseDI	GACNNNNNNGTC	1 cut(s)	10
EaeI	YGGCCR	2 cut(s)	158, 503
EciI	GGCGGA	3 cut(s)	190, 217, 244
Eco130I	CCWWGG	2 cut(s)	240, 1179
Eco147I	AGGCCT	1 cut(s)	658
Eco47I	GGWCC	3 cut(s)	44, 50, 466
Eco57I	CTGAAG	2 cut(s)	123, 938
Eco88I	CYCGRG	3 cut(s)	765, 801, 849
EcoO109I	RGGNCCY	2 cut(s)	44, 466
EcoRI	GAATTC	1 cut(s)	379
EcoRII	CCWGG	2 cut(s)	46, 143
EcoT14I	CCWWGG	2 cut(s)	240, 1179
EcoT22I	ATGCAT	1 cut(s)	512
ErhI	CCWWGG	2 cut(s)	240, 1179
FaeI	CATG	8 cut(s)	217, 244, 348, 510, 530, 665, 1183, 1243
FaqI	GGGAC	2 cut(s)	36, 479
FatI	CATG	8 cut(s)	213, 240, 344, 506, 526, 661, 1179, 1239
Fnu4HI	GCNGC	4 cut(s)	161, 810, 1085, 1128
FokI	GGATG	4 cut(s)	104, 407, 765, 782
Fsp4HI	GCNGC	4 cut(s)	161, 810, 1085, 1128
FspBI	CTAG	2 cut(s)	462, 1257
FspI	TGCGCA	1 cut(s)	860
GlaI	GCGC	2 cut(s)	108, 860
GluI	GCNGC	4 cut(s)	161, 810, 1085, 1128
HaeII	RGCGCY	1 cut(s)	110
HaeIII	GGCC	4 cut(s)	143, 160, 505, 658
HapII	CCGG	3 cut(s)	294, 719, 802
HgaI	GACGC	1 cut(s)	341
HhaI	GCGC	2 cut(s)	109, 861
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	8 cut(s)	217, 244, 348, 510, 530, 665, 1183, 1243
Hin6I	GCGC	2 cut(s)	107, 859
HinP1I	GCGC	2 cut(s)	107, 859
HincII	GTYRAC	1 cut(s)	265
HindII	GTYRAC	1 cut(s)	265
HinfI	GANTC	9 cut(s)	37, 632, 733, 763, 838, 1109, 1175, 1220, 1253
HpaII	CCGG	3 cut(s)	294, 719, 802
HphI	GGTGA	2 cut(s)	308, 577
Hpy166II	GTNNAC	1 cut(s)	265
Hpy188I	TCNGA	7 cut(s)	16, 385, 699, 787, 843, 1144, 1237
Hpy188III	TCNNGA	6 cut(s)	53, 767, 849, 1073, 1206, 1227
Hpy8I	GTNNAC	1 cut(s)	265
Hpy99I	CGWCG	1 cut(s)	672
HpyAV	CCTTC	1 cut(s)	966
HpyCH4III	ACNGT	3 cut(s)	518, 1061, 1247
HpyCH4IV	ACGT	4 cut(s)	11, 612, 667, 918
HpyF10VI	GCNNNNNNNGC	1 cut(s)	865
HpyF3I	CTNAG	4 cut(s)	384, 653, 792, 968
HpySE526I	ACGT	4 cut(s)	11, 612, 667, 918
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	8 cut(s)	217, 244, 348, 510, 530, 665, 1183, 1243
HspAI	GCGC	2 cut(s)	107, 859
KflI	GGGWCCC	1 cut(s)	466
Kzo9I	GATC	1 cut(s)	645
LmnI	GCTCC	1 cut(s)	426
Lsp1109I	GCAGC	3 cut(s)	821, 1096, 1139
LweI	GCATC	4 cut(s)	7, 46, 82, 526
MaeI	CTAG	2 cut(s)	462, 1257
MaeII	ACGT	4 cut(s)	11, 612, 667, 918
MaeIII	GTNAC	7 cut(s)	272, 329, 475, 568, 583, 919, 1055
MalI	GATC	1 cut(s)	647
MboI	GATC	1 cut(s)	645
MboII	GAAGA	9 cut(s)	329, 589, 993, 1194, 1221, 1224, 1281, 1284, 1287
MflI	RGATCY	1 cut(s)	645
MhlI	GDGCHC	1 cut(s)	1264
MlsI	TGGCCA	1 cut(s)	505
MluCI	AATT	7 cut(s)	85, 120, 254, 379, 823, 1138, 1170
MluNI	TGGCCA	1 cut(s)	505
MlyI	GAGTC	2 cut(s)	626, 757
Mox20I	TGGCCA	1 cut(s)	505
Mph1103I	ATGCAT	1 cut(s)	512
MroXI	GAANNNNTTC	1 cut(s)	1219
MscI	TGGCCA	1 cut(s)	505
MseI	TTAA	4 cut(s)	642, 830, 905, 1281
MslI	CAYNNNNRTG	1 cut(s)	1163
Msp20I	TGGCCA	1 cut(s)	505
MspA1I	CMGCKG	3 cut(s)	926, 1130, 1163
MspCI	CTTAAG	1 cut(s)	641
MspI	CCGG	3 cut(s)	294, 719, 802
MspR9I	CCNGG	5 cut(s)	48, 145, 294, 802, 803
Mva1269I	GAATGC	1 cut(s)	551
MvaI	CCWGG	2 cut(s)	48, 145
MwoI	GCNNNNNNNGC	1 cut(s)	865
NciI	CCSGG	3 cut(s)	294, 802, 803
NcoI	CCATGG	2 cut(s)	240, 1179
NdeII	GATC	1 cut(s)	645
NlaIII	CATG	8 cut(s)	217, 244, 348, 510, 530, 665, 1183, 1243
NlaIV	GGNNCC	4 cut(s)	45, 52, 467, 468
NmuCI	GTSAC	4 cut(s)	329, 475, 583, 919
NsbI	TGCGCA	1 cut(s)	860
NsiI	ATGCAT	1 cut(s)	512
NspI	RCATGY	2 cut(s)	348, 530
OliI	CACNNNNGTG	1 cut(s)	1163
PaeR7I	CTCGAG	1 cut(s)	849
PceI	AGGCCT	1 cut(s)	658
PciI	ACATGT	1 cut(s)	344
PctI	GAATGC	1 cut(s)	551
PdmI	GAANNNNTTC	1 cut(s)	1219
PfeI	GAWTC	7 cut(s)	37, 733, 838, 1109, 1175, 1220, 1253
PflMI	CCANNNNNTGG	1 cut(s)	596
PkrI	GCNGC	4 cut(s)	162, 811, 1086, 1129
PleI	GAGTC	2 cut(s)	626, 757
PpsI	GAGTC	2 cut(s)	626, 757
PpuMI	RGGWCCY	2 cut(s)	44, 466
PscI	ACATGT	1 cut(s)	344
PsiI	TTATAA	1 cut(s)	1136
Psp5II	RGGWCCY	2 cut(s)	44, 466
Psp6I	CCWGG	2 cut(s)	46, 143
PspGI	CCWGG	2 cut(s)	46, 143
PspN4I	GGNNCC	4 cut(s)	45, 52, 467, 468
PspPI	GGNCC	4 cut(s)	44, 50, 141, 466
PspPPI	RGGWCCY	2 cut(s)	44, 466
PstNI	CAGNNNCTG	1 cut(s)	791
PsuI	RGATCY	1 cut(s)	645
PvuII	CAGCTG	2 cut(s)	1130, 1163
RsaI	GTAC	2 cut(s)	283, 882
RsaNI	GTAC	2 cut(s)	282, 881
RseI	CAYNNNNRTG	1 cut(s)	1163
SaqAI	TTAA	4 cut(s)	642, 830, 905, 1281
SatI	GCNGC	4 cut(s)	161, 810, 1085, 1128
Sau3AI	GATC	1 cut(s)	645
Sau96I	GGNCC	4 cut(s)	44, 50, 141, 466
SchI	GAGTC	2 cut(s)	626, 757
ScrFI	CCNGG	5 cut(s)	48, 145, 294, 802, 803
SduI	GDGCHC	1 cut(s)	1264
SfaNI	GCATC	4 cut(s)	7, 46, 82, 526
Sfr274I	CTCGAG	1 cut(s)	849
SinI	GGWCC	3 cut(s)	44, 50, 466
SlaI	CTCGAG	1 cut(s)	849
SmaI	CCCGGG	1 cut(s)	803
SmiMI	CAYNNNNRTG	1 cut(s)	1163
SmlI	CTYRAG	4 cut(s)	277, 641, 849, 1117
SmoI	CTYRAG	4 cut(s)	277, 641, 849, 1117
Sse9I	AATT	7 cut(s)	85, 120, 254, 379, 823, 1138, 1170
SseBI	AGGCCT	1 cut(s)	658
SsiI	CCGC	9 cut(s)	115, 125, 161, 175, 202, 229, 411, 924, 946
SspI	AATATT	4 cut(s)	181, 208, 235, 445
SspMI	CTAG	2 cut(s)	462, 1257
StuI	AGGCCT	1 cut(s)	658
StyD4I	CCNGG	5 cut(s)	46, 143, 292, 800, 801
StyI	CCWWGG	2 cut(s)	240, 1179
TaaI	ACNGT	3 cut(s)	518, 1061, 1247
TaiI	ACGT	4 cut(s)	14, 615, 670, 921
TaqI	TCGA	2 cut(s)	435, 850
TasI	AATT	7 cut(s)	85, 120, 254, 379, 823, 1138, 1170
TatI	WGTACW	1 cut(s)	281
TauI	GCSGC	1 cut(s)	163
TfiI	GAWTC	7 cut(s)	37, 733, 838, 1109, 1175, 1220, 1253
Tru1I	TTAA	4 cut(s)	642, 830, 905, 1281
Tru9I	TTAA	4 cut(s)	642, 830, 905, 1281
TscAI	CASTG	3 cut(s)	424, 882, 1252
TseFI	GTSAC	4 cut(s)	329, 475, 583, 919
TseI	GCWGC	3 cut(s)	809, 1084, 1127
Tsp45I	GTSAC	4 cut(s)	329, 475, 583, 919
TspDTI	ATGAA	6 cut(s)	38, 90, 267, 590, 767, 1188
TspMI	CCCGGG	1 cut(s)	801
TspRI	CASTG	3 cut(s)	424, 882, 1252
Van91I	CCANNNNNTGG	1 cut(s)	596
Vha464I	CTTAAG	1 cut(s)	641
VpaK11BI	GGWCC	3 cut(s)	44, 50, 466
XapI	RAATTY	3 cut(s)	85, 379, 1138
XceI	RCATGY	2 cut(s)	348, 530
XhoI	CTCGAG	1 cut(s)	849
XmaI	CCCGGG	1 cut(s)	801
XmnI	GAANNNNTTC	1 cut(s)	1219
XspI	CTAG	2 cut(s)	462, 1257
ZraI	GACGTC	1 cut(s)	12
Zsp2I	ATGCAT	1 cut(s)	512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.