MD14G1060400.v1.1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013204
Physical Location & Seq
Reverse (-)
6379869 .. 6383321
3453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1060400.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCGGAAGAAGATGATTACATGGGCGACCTCTCTCAGTTCCTCACTCCAGAAGTCACCAGCCCTCCAAAAGCTTCATCCAAAATGATTTTGAATACCAAAAACCCAAGTAATTTTCAACCCTCGAACAAAAAATCTAAACTGCTAAACTGGCAAGAACAACGTAAAATCGATAGGGAGCGAAAGCAACAGGAAGAGGATGAGAAAACGTTGGCTAAAATAGAGACTGCTCCAATTCCACAATCCAACATTGGATTCAAACTTTTGAAGCAAATGGGGTATACCCCGGGTTCCGCACTTGGTAAGGAGGGCTTGGGCCAGGCTGAGCCGGTGAAGATTGACATTCGGCGCTCACGGGCTGGGATTGGGCGGGAGGATCCGCATAAGGAGAAGAGGAAGCGAGAGGAGATAAGGACTTGGGCGGAAAGGAGGACCGAGGAGGCTATGATGGAGGAGTTTGGGTCTAGGCAGAAGTCACAGTGGCGGAACAAGAGAGTTGTGGTTAATTTTAAGAAGGCACAGGCAGCGTTGGATCAATTGGAGAATAAGGAAGTTGTGCCTCCGGAGAAGAATGAGAACGGGGAAGAGGAAGGGGGAGAGGAGGAAGAGGAAGAAGAAGAAATTACAGAAGAGGATTTACATGAGATATTGATGAAACTGAGAGATGAACATAGGTACTGCCTCTTTTGTGGATGCCAGTATGAATCAACGGAGGCACTATTGTCGTACTGCCCTGGAACTGAGGAAGACGACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4187 G-patch

Protein Analysis

253

Amino Acids

29.22

Weight (kDa)

5.02

Isoelectric Point (pI)

75.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 68 - 125 1.2e-06 G-patch domain
G-patch PF01585 82 - 124 5.3e-14 G-patch domain
DUF4187 PF13821 200 - 252 6.6e-18 Domain of unknown function (DUF4187)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57910
fragaria_vesca FvH4_6g22250 FvH4_6g22650
malus_domestica MD14G1060400.v1.1
prunus_persica Prupe.7G074100_v2.0.a1 Prupe.7G074100_v2.0.a1
pyrus_communis pycom14g04890
rosa_chinensis RchiOBHm_Chr3g0480211
rosa_laevigata RLG00000023510
rosa_multiflora Rmu_sc0006148.1_g000003 Rmu_sc0032341.1_g000002
rosa_roxburghii Rroxscaffold_6G00401700
rosa_rugosa Rorug03G0181100
rosa_samantha Rh3AG232900 Rh3BG265500 Rh3CG262700 Rh3DG258700
rosa_wichuraiana Rw3G020920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 6 cut(s) 5, 294, 370, 380, 422, 484
AclI AACGTT 1 cut(s) 209
AclWI GGATC 3 cut(s) 371, 384, 540
AfaI GTAC 2 cut(s) 677, 728
AgsI TTSAA 4 cut(s) 94, 119, 259, 268
AjnI CCWGG 2 cut(s) 318, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 3 cut(s) 371, 384, 540
Ama87I CYCGRG 1 cut(s) 286
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 316, 432
AsuC2I CCSGG 2 cut(s) 287, 288
AsuHPI GGTGA 2 cut(s) 49, 343
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 376
BarI GAAGNNNNNNTAC 2 cut(s) 621, 653
BbsI GAAGAC 1 cut(s) 753
BbvI GCAGC 1 cut(s) 536
BccI CCATC 1 cut(s) 442
BcgI CGANNNNNNTGC 2 cut(s) 705, 739
BciT130I CCWGG 2 cut(s) 320, 735
BcnI CCSGG 2 cut(s) 287, 288
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 1 cut(s) 525
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 526
Bme1390I CCNGG 4 cut(s) 287, 288, 320, 735
Bme18I GGWCC 1 cut(s) 432
BmeT110I CYCGRG 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 316, 432
BmiI GGNNCC 2 cut(s) 292, 378
BmrFI CCNGG 4 cut(s) 287, 288, 320, 735
BmsI GCATC 1 cut(s) 683
BpiI GAAGAC 1 cut(s) 753
BpmI CTGGAG 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 324
BpuMI CCSGG 2 cut(s) 287, 288
Bsa29I ATCGAT 1 cut(s) 171
BsaJI CCNNGG 4 cut(s) 285, 286, 435, 733
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 446, 476
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 155, 697
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 2 cut(s) 320, 735
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 4 cut(s) 285, 286, 435, 733
BseGI GGATG 3 cut(s) 77, 205, 698
BseMII CTCAG 4 cut(s) 50, 315, 650, 732
BseNI ACTGG 2 cut(s) 155, 697
BseRI GAGGAG 4 cut(s) 419, 452, 467, 614
BseXI GCAGC 1 cut(s) 536
BseYI CCCAGC 1 cut(s) 359
BshFI GGCC 1 cut(s) 318
BshVI ATCGAT 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 3 cut(s) 287, 329, 563
BsmAI GTCTC 1 cut(s) 218
BsnI GGCC 1 cut(s) 318
BsoBI CYCGRG 1 cut(s) 286
Bsp13I TCCGGA 1 cut(s) 562
Bsp143I GATC 2 cut(s) 376, 532
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 6 cut(s) 5, 294, 370, 380, 422, 484
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 4 cut(s) 49, 316, 651, 733
BspDI ATCGAT 1 cut(s) 171
BspEI TCCGGA 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 292, 378
BspPI GGATC 3 cut(s) 371, 384, 540
BsrFI RCCGGY 1 cut(s) 328
BsrI ACTGG 2 cut(s) 155, 697
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 4 cut(s) 285, 286, 435, 733
BssMI GATC 2 cut(s) 376, 532
BssNAI GTATAC 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 320, 735
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 5 cut(s) 189, 386, 579, 600, 624
BstDEI CTNAG 4 cut(s) 36, 324, 659, 741
BstF5I GGATG 3 cut(s) 77, 205, 698
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 2 cut(s) 379, 535
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 2 cut(s) 376, 532
BstMWI GCNNNNNNNGC 2 cut(s) 151, 524
BstNI CCWGG 2 cut(s) 320, 735
BstSCI CCNGG 4 cut(s) 285, 286, 318, 733
BstV1I GCAGC 1 cut(s) 536
BstV2I GAAGAC 1 cut(s) 753
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BstZ17I GTATAC 1 cut(s) 282
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 1 cut(s) 318
BsuTUI ATCGAT 1 cut(s) 171
BtsCI GGATG 3 cut(s) 77, 205, 698
BtsIMutI CAGTG 2 cut(s) 485, 754
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 2 cut(s) 316, 432
Cfr9I CCCGGG 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 171
Csp6I GTAC 2 cut(s) 676, 727
CviAII CATG 2 cut(s) 22, 641
CviJI RGCY 9 cut(s) 63, 74, 215, 312, 318, 323, 328, 359, 443
CviKI_1 RGCY 9 cut(s) 63, 74, 215, 312, 318, 323, 328, 359, 443
CviQI GTAC 2 cut(s) 676, 727
DdeI CTNAG 4 cut(s) 36, 324, 659, 741
DpnI GATC 2 cut(s) 378, 534
DpnII GATC 2 cut(s) 376, 532
Eam1104I CTCTTC 5 cut(s) 189, 386, 579, 600, 624
EarI CTCTTC 5 cut(s) 189, 386, 579, 600, 624
EciI GGCGGA 3 cut(s) 20, 437, 499
Eco47I GGWCC 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 318, 733
FaeI CATG 2 cut(s) 25, 644
FaiI YATR 7 cut(s) 23, 282, 384, 446, 642, 672, 702
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 2 cut(s) 21, 640
FauI CCCGC 1 cut(s) 363
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 3 cut(s) 64, 212, 705
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 350
GluI GCNGC 1 cut(s) 525
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 1 cut(s) 318
HapII CCGG 3 cut(s) 287, 329, 563
HhaI GCGC 1 cut(s) 351
Hin1II CATG 2 cut(s) 25, 644
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 72
HinfI GANTC 2 cut(s) 255, 704
HpaII CCGG 3 cut(s) 287, 329, 563
HphI GGTGA 2 cut(s) 49, 343
Hpy166II GTNNAC 1 cut(s) 282
Hpy188III TCNNGA 2 cut(s) 50, 563
Hpy8I GTNNAC 1 cut(s) 282
HpyAV CCTTC 2 cut(s) 508, 584
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 2 cut(s) 163, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 524
HpyF3I CTNAG 4 cut(s) 36, 324, 659, 741
HpySE526I ACGT 2 cut(s) 163, 209
Hsp92II CATG 2 cut(s) 25, 644
HspAI GCGC 1 cut(s) 349
Kpn2I TCCGGA 1 cut(s) 562
Kzo9I GATC 2 cut(s) 376, 532
LmnI GCTCC 2 cut(s) 178, 235
Lsp1109I GCAGC 1 cut(s) 536
LweI GCATC 1 cut(s) 683
MaeI CTAG 1 cut(s) 465
MaeII ACGT 2 cut(s) 163, 209
MaeIII GTNAC 2 cut(s) 55, 474
MalI GATC 2 cut(s) 378, 534
MboI GATC 2 cut(s) 376, 532
MfeI CAATTG 1 cut(s) 536
MflI RGATCY 1 cut(s) 376
MluCI AATT 5 cut(s) 112, 234, 505, 536, 621
MmeI TCCRAC 2 cut(s) 270, 510
MroI TCCGGA 1 cut(s) 562
MseI TTAA 2 cut(s) 504, 510
MspI CCGG 3 cut(s) 287, 329, 563
MspR9I CCNGG 4 cut(s) 287, 288, 320, 735
MunI CAATTG 1 cut(s) 536
MvaI CCWGG 2 cut(s) 320, 735
MwoI GCNNNNNNNGC 2 cut(s) 151, 524
NciI CCSGG 2 cut(s) 287, 288
NdeII GATC 2 cut(s) 376, 532
NlaIII CATG 2 cut(s) 25, 644
NlaIV GGNNCC 2 cut(s) 292, 378
NmuCI GTSAC 2 cut(s) 55, 474
PfeI GAWTC 2 cut(s) 255, 704
PkrI GCNGC 1 cut(s) 526
Psp1406I AACGTT 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 318, 733
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 2 cut(s) 318, 733
PspN4I GGNNCC 2 cut(s) 292, 378
PspPI GGNCC 2 cut(s) 316, 432
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 2 cut(s) 677, 728
RsaNI GTAC 2 cut(s) 676, 727
SaqAI TTAA 2 cut(s) 504, 510
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 2 cut(s) 376, 532
Sau96I GGNCC 2 cut(s) 316, 432
ScrFI CCNGG 4 cut(s) 287, 288, 320, 735
SetI ASST 5 cut(s) 33, 76, 166, 212, 677
SfaNI GCATC 1 cut(s) 683
SinI GGWCC 1 cut(s) 432
SmaI CCCGGG 1 cut(s) 288
Sse9I AATT 5 cut(s) 112, 234, 505, 536, 621
SsiI CCGC 6 cut(s) 5, 294, 370, 380, 422, 484
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 4 cut(s) 285, 286, 318, 733
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 2 cut(s) 166, 212
TaqI TCGA 2 cut(s) 125, 171
TaqII GACCGA 1 cut(s) 449
TasI AATT 5 cut(s) 112, 234, 505, 536, 621
TfiI GAWTC 2 cut(s) 255, 704
Tru1I TTAA 2 cut(s) 504, 510
Tru9I TTAA 2 cut(s) 504, 510
TscAI CASTG 1 cut(s) 485
TseFI GTSAC 2 cut(s) 55, 474
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 2 cut(s) 55, 474
TspDTI ATGAA 4 cut(s) 66, 668, 681, 717
TspGWI ACGGA 1 cut(s) 725
TspMI CCCGGG 1 cut(s) 286
TspRI CASTG 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 432
XmaI CCCGGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 281
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.