MD14G1083400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012639
Physical Location & Seq
Forward (+)
9851455 .. 9853089
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1083400.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGTTGTGGAATTTAATGATGAAGTCCCATGTTGAATGTGGTCTTGTTGATTCGGCTCTGTTGCTGTACAAGAAGATGCGTGAGTTGGGTGTTTCGCATGATTGCTTTACGTTCCCGATTGTAAACAGGGTGGTTGTGTTGCTTGGGGGTGAAGTTGGGTATGCAGGAATGGTTCACTGTGTGGCAATTCAAATGGGTTTTGGAATGGATATGTATTTTGGTAATACAATGATTGATTTGTATGTGAAATGCGGAGCAATTGATCATGCTCGTAAGTTGTTTGATGAAATGTGTCAAAGAGATTTGGTTTCATGGACATCGATGATTTCCGGGCATGTTTCGGAGGGGAATGTTCCCAGTGGGTTGAGCTTGTTCAATGAAATGAGGTTGGAGTTGGAACCGAATTCCGTTACTATGTTGATCATGTTGCAAGGATGTTGTGGCACTGAAAGTGCAATATGTGGAAGTCAATTTCATGGTTATGTGATTAAGAATGGTTTGCTGTATGATGCATCTGTGCAGAACTCAATCTTGAGAATGTATGCTAAATTAGGTACAATCAACGAAGTTGAAGGCTTTTTCAGCGAGCTTGATAGAAGGGATGTCGTCTCCTGGAATATCTGCATTTCCATTTTTTCTTCCAGAGGGGATGTTGCGAAAGTAAGAGAGTTGTTCAATGATATGCAGGGCAAAGTGGCGCCGGGCGTTGAGACATTAACTTTGGTTATATCAGCATTGACCAAACATGGGATTCTTTCCCAAGGTGAAAGCTTGCATTGTTTGGCGATAAAACGTGGGCTTTGTGATCATGTTTTGCAGACATCTTTGTTAGACCTCTATGCCAAGTGTGGAGAATTAGGAATCTCAGATAGGCTGTTCAGAGAAATCCCTCACAGGAACAGCATTACTTGGGGTGCAATGATGTTTGGCTTCATTCAAAATGGTTGGTTTAGTGAAGCTGTTGGACTCTTTCGCGAAATGCAAGCTTTGGGTCCGGAACCAAGGGCTGAGATACTAAGAAGCCTCGTTGATGCATTTGCAAACCTAGGTGCTTTGAAGTTGGGAAAACAAATTCATGGCTACATCATAAGGAAATCATTGTATGAAGACGAGGAAAGCTATACTCATCTCGAAACCTCCATAATAAACATGTACATAAGATGTGGTAGTCTATCTGCAGCTAGAGTGTGTTTTGATAGAATGTTGGTCAAAGATATAGTGACTTGGACATCAATGATTGAGGGCTGTGGAAGCCACGGACTTGGTTTTGAAGCCTTGAAACTATTTGACCTGATGATTAGAGAAGGAGTAAGACCAAATAGTGTCACCTTCATAAGCTTGTTATCTGCTTGTAGCCATTCTGGTCTTGTTACTGAAGGTTGTGATGCTTTTTGTTCCATGAAATGGAAGTTTGGTATTGAACCTGATTTAGACCATTACACTAGCATTGTCGATCTCTTGGGTCGATCTGGGAAGCTTAAAGAGGCCTTAGCTGATGTAGAATTTACAGTCTCAGAGATGTTGGGGAGTATGCTGCTCAGAGGCTCTTGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

519

Amino Acids

57.53

Weight (kDa)

5.71

Isoelectric Point (pI)

27.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 101 - 147 3.3e-10 PPR repeat family
PPR PF01535 103 - 129 7.8e-06 PPR repeat
PPR_2 PF13041 200 - 247 1.3e-06 PPR repeat family
PPR PF01535 302 - 331 4.2e-06 PPR repeat
PPR_2 PF13041 404 - 452 5.8e-12 PPR repeat family
PPR PF01535 407 - 437 9.4e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24950
malus_domestica MD05G1013600.v1.1 MD14G1083400.v1.1
prunus_persica Prupe.7G047100_v2.0.a1
pyrus_communis pycom14g07240
rosa_chinensis RchiOBHm_Chr2g0124611
rosa_laevigata RLG00000018808
rosa_multiflora Rmu_sc0005597.1_g000009
rosa_roxburghii Rroxscaffold_2G00119840
rosa_rugosa Rorug02G0251600 Rorug02G0251700
rosa_samantha Rh2AG310300 Rh2AG310400 Rh2BG318800 Rh2CG297200 Rh2CG302100 Rh2DG334000
rosa_wichuraiana Rw0G009400 Rw2G025100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 697
AccB7I CCANNNNNTGG 1 cut(s) 1404
AccII CGCG 1 cut(s) 975
AccIII TCCGGA 1 cut(s) 994
AciI CCGC 1 cut(s) 252
AcsI RAATTY 4 cut(s) 10, 403, 1071, 1502
AcuI CTGAAG 1 cut(s) 1395
AcyI GRCGYC 1 cut(s) 698
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 3 cut(s) 68, 556, 1154
AfiI CCNNNNNNNGG 2 cut(s) 747, 1404
AflIII ACRYGT 1 cut(s) 1149
AjnI CCWGG 1 cut(s) 611
Alw26I GTCTC 3 cut(s) 613, 704, 1516
Aor13HI TCCGGA 1 cut(s) 994
AoxI GGCC 1 cut(s) 1485
ApeKI GCWGC 2 cut(s) 1178, 1534
ApoI RAATTY 4 cut(s) 10, 403, 1071, 1502
ArsI GACNNNNNNTTYG 2 cut(s) 703, 735
AspA2I CCTAGG 1 cut(s) 1045
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 1 cut(s) 992
AsuC2I CCSGG 2 cut(s) 331, 702
AsuHPI GGTGA 3 cut(s) 161, 776, 1318
AvaII GGWCC 1 cut(s) 992
AvrII CCTAGG 1 cut(s) 1045
BanI GGYRCC 1 cut(s) 697
BbsI GAAGAC 1 cut(s) 1113
BbvI GCAGC 2 cut(s) 1190, 1521
BciT130I CCWGG 1 cut(s) 613
BclI TGATCA 3 cut(s) 262, 420, 805
BcnI CCSGG 2 cut(s) 331, 702
BcoDI GTCTC 3 cut(s) 613, 704, 1516
BfaI CTAG 4 cut(s) 1046, 1182, 1443, 1555
BfmI CTRYAG 1 cut(s) 1176
BfoI RGCGCY 1 cut(s) 701
BisI GCNGC 2 cut(s) 1179, 1535
BlnI CCTAGG 1 cut(s) 1045
BlsI GCNGC 2 cut(s) 1180, 1536
Bme1390I CCNGG 3 cut(s) 331, 613, 702
Bme18I GGWCC 1 cut(s) 992
BmgT120I GGNCC 1 cut(s) 992
BmiI GGNNCC 4 cut(s) 399, 699, 993, 999
BmrFI CCNGG 3 cut(s) 331, 613, 702
BmrI ACTGGG 1 cut(s) 351
BmsI GCATC 5 cut(s) 66, 499, 521, 1021, 1375
BmuI ACTGGG 1 cut(s) 351
BpiI GAAGAC 1 cut(s) 1113
Bpu10I CCTNAGC 1 cut(s) 1489
BpuEI CTTGAG 1 cut(s) 553
BpuMI CCSGG 2 cut(s) 331, 702
Bsa29I ATCGAT 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 698
BsaJI CCNNGG 4 cut(s) 760, 1001, 1045, 1255
BsaWI WCCGGW 1 cut(s) 994
Bsc4I CCNNNNNNNGG 2 cut(s) 747, 1404
Bse1I ACTGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 924
BseAI TCCGGA 1 cut(s) 994
BseBI CCWGG 1 cut(s) 613
BseCI ATCGAT 1 cut(s) 320
BseDI CCNNGG 4 cut(s) 760, 1001, 1045, 1255
BseGI GGATG 3 cut(s) 440, 607, 655
BseLI CCNNNNNNNGG 2 cut(s) 747, 1404
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 4 cut(s) 879, 999, 1527, 1552
BseNI ACTGG 1 cut(s) 357
BseXI GCAGC 2 cut(s) 1190, 1521
BsgI GTGCAG 1 cut(s) 539
Bsh1236I CGCG 1 cut(s) 975
BshFI GGCC 1 cut(s) 1487
BshNI GGYRCC 1 cut(s) 697
BshVI ATCGAT 1 cut(s) 320
BsiSI CCGG 3 cut(s) 330, 701, 995
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 2 cut(s) 747, 1404
BsmAI GTCTC 3 cut(s) 613, 704, 1516
BsmBI CGTCTC 1 cut(s) 613
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 1 cut(s) 1487
Bsp13I TCCGGA 1 cut(s) 994
Bsp1407I TGTACA 2 cut(s) 66, 1152
Bsp143I GATC 5 cut(s) 262, 420, 805, 1453, 1466
Bsp68I TCGCGA 1 cut(s) 975
BspACI CCGC 1 cut(s) 252
BspANI GGCC 1 cut(s) 1487
BspCNI CTCAG 4 cut(s) 878, 1000, 1526, 1551
BspDI ATCGAT 1 cut(s) 320
BspEI TCCGGA 1 cut(s) 994
BspFNI CGCG 1 cut(s) 975
BspLI GGNNCC 4 cut(s) 399, 699, 993, 999
BspMAI CTGCAG 1 cut(s) 1180
BspT107I GGYRCC 1 cut(s) 697
BsrDI GCAATG 1 cut(s) 924
BsrGI TGTACA 2 cut(s) 66, 1152
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 4 cut(s) 760, 1001, 1045, 1255
BssMI GATC 5 cut(s) 262, 420, 805, 1453, 1466
BssNI GRCGYC 1 cut(s) 698
BssT1I CCWWGG 3 cut(s) 760, 1001, 1045
Bst2UI CCWGG 1 cut(s) 613
Bst4CI ACNGT 2 cut(s) 179, 1510
BstACI GRCGYC 1 cut(s) 698
BstAUI TGTACA 2 cut(s) 66, 1152
BstC8I GCNNGC 3 cut(s) 587, 773, 984
BstDEI CTNAG 6 cut(s) 865, 1008, 1016, 1489, 1513, 1538
BstDSI CCRYGG 1 cut(s) 1255
BstF5I GGATG 3 cut(s) 440, 607, 655
BstFNI CGCG 1 cut(s) 975
BstH2I RGCGCY 1 cut(s) 701
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 5 cut(s) 265, 423, 808, 1456, 1469
BstMAI GTCTC 3 cut(s) 613, 704, 1516
BstMBI GATC 5 cut(s) 262, 420, 805, 1453, 1466
BstMWI GCNNNNNNNGC 2 cut(s) 582, 1251
BstNI CCWGG 1 cut(s) 613
BstNSI RCATGY 2 cut(s) 338, 1153
BstSCI CCNGG 3 cut(s) 329, 611, 700
BstSFI CTRYAG 1 cut(s) 1176
BstUI CGCG 1 cut(s) 975
BstV1I GCAGC 2 cut(s) 1190, 1521
BstV2I GAAGAC 1 cut(s) 1113
BstXI CCANNNNNNTGG 1 cut(s) 1262
Bsu15I ATCGAT 1 cut(s) 320
BsuRI GGCC 1 cut(s) 1487
BsuTUI ATCGAT 1 cut(s) 320
BtgI CCRYGG 1 cut(s) 1255
BtsCI GGATG 3 cut(s) 440, 607, 655
BtsIMutI CAGTG 3 cut(s) 175, 364, 444
BtuMI TCGCGA 1 cut(s) 975
Cac8I GCNNGC 3 cut(s) 587, 773, 984
CfoI GCGC 1 cut(s) 700
Cfr13I GGNCC 1 cut(s) 992
ClaI ATCGAT 1 cut(s) 320
Csp6I GTAC 3 cut(s) 67, 555, 1153
CviQI GTAC 3 cut(s) 67, 555, 1153
DdeI CTNAG 6 cut(s) 865, 1008, 1016, 1489, 1513, 1538
DinI GGCGCC 1 cut(s) 699
DpnI GATC 5 cut(s) 264, 422, 807, 1455, 1468
DpnII GATC 5 cut(s) 262, 420, 805, 1453, 1466
DraIII CACNNNGTG 1 cut(s) 181
Eco130I CCWWGG 3 cut(s) 760, 1001, 1045
Eco147I AGGCCT 1 cut(s) 1487
Eco47I GGWCC 1 cut(s) 992
Eco57I CTGAAG 1 cut(s) 1395
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 611
EcoT14I CCWWGG 3 cut(s) 760, 1001, 1045
EcoT22I ATGCAT 2 cut(s) 514, 1036
EgeI GGCGCC 1 cut(s) 699
EheI GGCGCC 1 cut(s) 699
ErhI CCWWGG 3 cut(s) 760, 1001, 1045
Esp3I CGTCTC 1 cut(s) 613
FaqI GGGAC 1 cut(s) 10
FbaI TGATCA 3 cut(s) 262, 420, 805
Fnu4HI GCNGC 2 cut(s) 1179, 1535
FokI GGATG 3 cut(s) 447, 614, 662
Fsp4HI GCNGC 2 cut(s) 1179, 1535
FspBI CTAG 4 cut(s) 1046, 1182, 1443, 1555
GlaI GCGC 1 cut(s) 699
GluI GCNGC 2 cut(s) 1179, 1535
HaeII RGCGCY 1 cut(s) 701
HaeIII GGCC 1 cut(s) 1487
HapII CCGG 3 cut(s) 330, 701, 995
HhaI GCGC 1 cut(s) 700
Hin1I GRCGYC 1 cut(s) 698
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HindIII AAGCTT 4 cut(s) 769, 984, 1336, 1475
HinfI GANTC 4 cut(s) 50, 751, 861, 966
HpaII CCGG 3 cut(s) 330, 701, 995
HphI GGTGA 3 cut(s) 161, 776, 1318
Hpy166II GTNNAC 2 cut(s) 124, 175
Hpy188I TCNGA 5 cut(s) 343, 868, 881, 1516, 1541
Hpy188III TCNNGA 6 cut(s) 115, 532, 642, 974, 995, 1130
Hpy8I GTNNAC 2 cut(s) 124, 175
HpyAV CCTTC 5 cut(s) 566, 591, 1298, 1339, 1370
HpyCH4III ACNGT 2 cut(s) 179, 1510
HpyCH4IV ACGT 2 cut(s) 110, 793
HpyF10VI GCNNNNNNNGC 2 cut(s) 582, 1251
HpyF3I CTNAG 6 cut(s) 865, 1008, 1016, 1489, 1513, 1538
HpySE526I ACGT 2 cut(s) 110, 793
Hsp92I GRCGYC 1 cut(s) 698
HspAI GCGC 1 cut(s) 698
KasI GGCGCC 1 cut(s) 697
Kpn2I TCCGGA 1 cut(s) 994
Ksp22I TGATCA 3 cut(s) 262, 420, 805
Kzo9I GATC 5 cut(s) 262, 420, 805, 1453, 1466
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 2 cut(s) 1190, 1521
LweI GCATC 5 cut(s) 66, 499, 521, 1021, 1375
MaeI CTAG 4 cut(s) 1046, 1182, 1443, 1555
MaeII ACGT 2 cut(s) 110, 793
MaeIII GTNAC 4 cut(s) 409, 1219, 1324, 1369
MalI GATC 5 cut(s) 264, 422, 807, 1455, 1468
MboI GATC 5 cut(s) 262, 420, 805, 1453, 1466
MboII GAAGA 3 cut(s) 85, 630, 1118
MfeI CAATTG 1 cut(s) 258
MluCI AATT 9 cut(s) 10, 186, 258, 403, 470, 548, 854, 1071, 1502
Mly113I GGCGCC 1 cut(s) 698
MlyI GAGTC 1 cut(s) 960
MmeI TCCRAC 3 cut(s) 369, 375, 943
Mph1103I ATGCAT 2 cut(s) 514, 1036
MroI TCCGGA 1 cut(s) 994
MseI TTAA 4 cut(s) 14, 489, 716, 1479
MslI CAYNNNNRTG 1 cut(s) 480
MspI CCGG 3 cut(s) 330, 701, 995
MspR9I CCNGG 3 cut(s) 331, 613, 702
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 1 cut(s) 613
MvnI CGCG 1 cut(s) 975
MwoI GCNNNNNNNGC 2 cut(s) 582, 1251
NarI GGCGCC 1 cut(s) 698
NciI CCSGG 2 cut(s) 331, 702
NdeII GATC 5 cut(s) 262, 420, 805, 1453, 1466
NlaIV GGNNCC 4 cut(s) 399, 699, 993, 999
NmuCI GTSAC 2 cut(s) 1219, 1324
NruI TCGCGA 1 cut(s) 975
NsiI ATGCAT 2 cut(s) 514, 1036
NspI RCATGY 2 cut(s) 338, 1153
PceI AGGCCT 1 cut(s) 1487
PciI ACATGT 1 cut(s) 1149
PfeI GAWTC 3 cut(s) 50, 751, 861
PflMI CCANNNNNTGG 1 cut(s) 1404
PfoI TCCNGGA 1 cut(s) 611
PkrI GCNGC 2 cut(s) 1180, 1536
PleI GAGTC 1 cut(s) 960
PluTI GGCGCC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 960
PscI ACATGT 1 cut(s) 1149
Psp6I CCWGG 1 cut(s) 611
PspGI CCWGG 1 cut(s) 611
PspN4I GGNNCC 4 cut(s) 399, 699, 993, 999
PspPI GGNCC 1 cut(s) 992
PstI CTGCAG 1 cut(s) 1180
RruI TCGCGA 1 cut(s) 975
RsaI GTAC 3 cut(s) 68, 556, 1154
RsaNI GTAC 3 cut(s) 67, 555, 1153
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 4 cut(s) 14, 489, 716, 1479
SatI GCNGC 2 cut(s) 1179, 1535
Sau3AI GATC 5 cut(s) 262, 420, 805, 1453, 1466
Sau96I GGNCC 1 cut(s) 992
SchI GAGTC 1 cut(s) 960
ScrFI CCNGG 3 cut(s) 331, 613, 702
SfaNI GCATC 5 cut(s) 66, 499, 521, 1021, 1375
SfcI CTRYAG 1 cut(s) 1176
SfoI GGCGCC 1 cut(s) 699
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
Sse9I AATT 9 cut(s) 10, 186, 258, 403, 470, 548, 854, 1071, 1502
SseBI AGGCCT 1 cut(s) 1487
SsiI CCGC 1 cut(s) 252
SspDI GGCGCC 1 cut(s) 697
SspMI CTAG 4 cut(s) 1046, 1182, 1443, 1555
StuI AGGCCT 1 cut(s) 1487
StyD4I CCNGG 3 cut(s) 329, 611, 700
StyI CCWWGG 3 cut(s) 760, 1001, 1045
TaaI ACNGT 2 cut(s) 179, 1510
TaiI ACGT 2 cut(s) 113, 796
TaqI TCGA 4 cut(s) 320, 1131, 1452, 1465
TasI AATT 9 cut(s) 10, 186, 258, 403, 470, 548, 854, 1071, 1502
TatI WGTACW 2 cut(s) 66, 1152
TfiI GAWTC 3 cut(s) 50, 751, 861
Tru1I TTAA 4 cut(s) 14, 489, 716, 1479
Tru9I TTAA 4 cut(s) 14, 489, 716, 1479
TscAI CASTG 3 cut(s) 182, 364, 451
TseFI GTSAC 2 cut(s) 1219, 1324
TseI GCWGC 2 cut(s) 1178, 1534
Tsp45I GTSAC 2 cut(s) 1219, 1324
TspGWI ACGGA 2 cut(s) 397, 1272
TspRI CASTG 3 cut(s) 182, 364, 451
Van91I CCANNNNNTGG 1 cut(s) 1404
VpaK11BI GGWCC 1 cut(s) 992
XapI RAATTY 4 cut(s) 10, 403, 1071, 1502
XceI RCATGY 2 cut(s) 338, 1153
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XmaJI CCTAGG 1 cut(s) 1045
XspI CTAG 4 cut(s) 1046, 1182, 1443, 1555
Zsp2I ATGCAT 2 cut(s) 514, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.