Rh2BG318800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012639
Physical Location & Seq
Reverse (-)
37978761 .. 37982842
4082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG318800.1

Sequence Viewer

Length: 1785 bp
ATGCATGCTCGTATTCTAGTCTCAACTGGGTTCAAGCCCCTCAATCTTTTTTCAAAGTTGGTTTCTTTGTATGCTCGGTTCAATGACCTTGGCAGCGCTGTTTCGGTTTTCAGTTCCCTACAAGAACCAAATACCATGCTGTGGAATCTAATCATAAGGACCCACATTGAATCGGGTCGTCTTGATTCGGCTCTGTTGTTGTACAGAAAGATGCGCGAGTTGGGTGTTTCACATGATTGTTTTACATTTCCAATTGTTAACAAGGCGGTCTTATTGGTTGGGGGTGATGTTAGATATGCAGGAATGGTTCATTCTGTGGCAATTCAAATGGGTTTTGGACTGGATGTGTATTTTGGCAATACTATGATTGAGGTGTATGTGAAATGCGGCAATCTCAGTTACGCCCGTACCATTGAAGAAGTCGAAGACTTTTTCAGGGAACTTGATAGGAGGGATGTTGTTTCCTGGAATATTTGTATTTCATCTTATACTTCGAGAGGGGATTTTGTGAAAGTAAGAGATCTGTTCAATGAGATGCAGGGTGGAGTAGCACCAAGTATTGAGACATTAACTATAGTTCTATCAGCATTGACCAAACATGGGATTCTATCCCAAGGTGAAAGCTTGCATGGTTTAGCAGTAAAAAGTGGTCTTCATGATGATGTTTTGCAGACATCTTTGTTGGACTTTTATGCCAAATGTGGAAAATTGGAAAGCTCGGATAAATTGTTTAGAGAACTCCCTGATAGAAACTGCATTACTTGCGGTGCAATGATGTTAGGACTCATTCATAATGGGTATTTCACTGAAGCGGTTGGAGTATTTAGACAAATGCAAGCTGCAGGTCTTGATCCGGGGGCAGAGATACTAAGAAACCTTATTGATGCATTTGCAAATTTAGGTGCTCTGAAGTTGGGAAAAGCAGTTCATGGCTACATAATAAGGAAATCATTTTGCGGAACTGAGGAAGGCCTTACTCATCTTGAAACCTCCATCTTAAACATGTACATAAGATGTGGTAGCATATCTACATCTAGAGTGTGTTTTAATAGAATGGTTTTCAAAGATGTGGTGGCATGGACATCAATGATTGAGGGTTATGGGAGCCATGGGCTTGGTTTTGAAGCCGCAAAACTCTTTGATCTAATGACAAGAGAAGGAATAAAGCCAAATAGTGTCACCTTCTTAAGCTTGTTATCTGCTTATAGCCACTCTGGTCTTGTGACTGAAGGCTGTGAAGCTTTTTATTACATGAAATGGAGGTTTGGAATTGAACCTGATATAGATCATTACACTTGTGTTGTTGATCTCTTGGGCAGATCTGGAAAGCTCAAAGAGGCCTTGGTTGTCATTTTGAAAATGTTGGCTTTTCCAGATAGTAGGATTTGGGGTGCTCTTCTTTCAGGATCTAAAATTTACAGCAACAGAGCACTTGGGCAATATGCAGCCCAGAGGCTCTTGGAATTAGAACCTGACTTACAGATGGTGATTTCCTTGTATGACATTATGACCATAGCCTTCTTAATTGAACGAAAGGAATTATCTCAGAGACCATCTACTAGCAAATTCATCGGGATGAGTGTTTGGGATTATCATCCCTGGAAAGTTGTAGCAGTATGCTGGAAAAGTAGACGTTGCTTTGTAAGGCTACAAGAACTCGCAACAGGGTTACAAAGCCAAGTGGCTAGTAAGCAACTAAGCAGTGCCTTTAATTGTTCTACTCCACTCCTATTACCTGAAATCCCACTATCAGGACTTTCAGACCGTGAAACAAGTCTGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

594

Amino Acids

66.21

Weight (kDa)

7.54

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 43 - 86 1.5e-06 PPR repeat family
PPR_2 PF13041 151 - 198 1.1e-08 PPR repeat family
PPR_2 PF13041 355 - 403 1.8e-11 PPR repeat family
PPR PF01535 358 - 388 4.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24950
malus_domestica MD05G1013600.v1.1 MD14G1083400.v1.1
prunus_persica Prupe.7G047100_v2.0.a1
pyrus_communis pycom14g07240
rosa_chinensis RchiOBHm_Chr2g0124611
rosa_laevigata RLG00000018808
rosa_multiflora Rmu_sc0005597.1_g000009
rosa_roxburghii Rroxscaffold_2G00119840
rosa_rugosa Rorug02G0251600 Rorug02G0251700
rosa_samantha Rh2AG310300 Rh2AG310400 Rh2BG318800 Rh2CG297200 Rh2CG302100 Rh2DG334000
rosa_wichuraiana Rw0G009400 Rw2G025100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 141
AccI GTMKAC 1 cut(s) 1630
AccII CGCG 1 cut(s) 216
AciI CCGC 6 cut(s) 266, 387, 765, 812, 957, 1128
AclWI GGATC 2 cut(s) 845, 1414
AcsI RAATTY 3 cut(s) 895, 1413, 1565
AcuI CTGAAG 3 cut(s) 828, 929, 1248
AfaI GTAC 3 cut(s) 203, 409, 1007
AfeI AGCGCT 1 cut(s) 97
AfiI CCNNNNNNNGG 3 cut(s) 141, 600, 1751
AflII CTTAAG 1 cut(s) 1186
AflIII ACRYGT 1 cut(s) 1002
AjnI CCWGG 2 cut(s) 464, 1598
AloI GAACNNNNNNTCC 2 cut(s) 909, 941
AluBI AGCT 6 cut(s) 624, 717, 839, 1191, 1241, 1330
AluI AGCT 6 cut(s) 624, 717, 839, 1191, 1241, 1330
Alw21I GWGCWC 3 cut(s) 907, 1396, 1432
Alw26I GTCTC 3 cut(s) 25, 557, 1543
AlwI GGATC 2 cut(s) 845, 1414
Aor51HI AGCGCT 1 cut(s) 97
AoxI GGCC 2 cut(s) 970, 1338
ApeKI GCWGC 3 cut(s) 93, 839, 1445
ApoI RAATTY 3 cut(s) 895, 1413, 1565
AspLEI GCGC 2 cut(s) 98, 216
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 4 cut(s) 296, 629, 1171, 1498
AvaII GGWCC 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 391, 424
BbsI GAAGAC 2 cut(s) 432, 644
Bbv12I GWGCWC 3 cut(s) 907, 1396, 1432
BbvI GCAGC 3 cut(s) 105, 826, 1457
BccI CCATC 3 cut(s) 1001, 1477, 1561
BcgI CGANNNNNNTGC 2 cut(s) 1552, 1586
BciT130I CCWGG 2 cut(s) 466, 1600
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 25, 557, 1543
BfaI CTAG 4 cut(s) 17, 1035, 1560, 1686
BfmI CTRYAG 2 cut(s) 573, 840
BfoI RGCGCY 1 cut(s) 99
BfrI CTTAAG 1 cut(s) 1186
BfuAI ACCTGC 1 cut(s) 833
BglII AGATCT 2 cut(s) 520, 1319
BisI GCNGC 5 cut(s) 94, 388, 840, 1128, 1446
BlsI GCNGC 5 cut(s) 95, 389, 841, 1129, 1447
Bme1390I CCNGG 3 cut(s) 466, 855, 1600
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 161, 1106
BmrFI CCNGG 3 cut(s) 466, 855, 1600
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 3 cut(s) 201, 525, 874
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 432, 644
BpuMI CCSGG 1 cut(s) 855
BsaBI GATNNNNATC 2 cut(s) 1284, 1593
BsaI GGTCTC 1 cut(s) 1543
BsaJI CCNNGG 6 cut(s) 88, 613, 854, 1108, 1341, 1598
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 600, 1751
Bse1I ACTGG 2 cut(s) 31, 345
Bse3DI GCAATG 1 cut(s) 777
Bse8I GATNNNNATC 2 cut(s) 1284, 1593
BseBI CCWGG 2 cut(s) 466, 1600
BseDI CCNNGG 6 cut(s) 88, 613, 854, 1108, 1341, 1598
BseGI GGATG 4 cut(s) 349, 460, 1581, 1594
BseJI GATNNNNATC 2 cut(s) 1284, 1593
BseLI CCNNNNNNNGG 3 cut(s) 141, 600, 1751
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 3 cut(s) 409, 954, 1559
BseNI ACTGG 2 cut(s) 31, 345
BseXI GCAGC 3 cut(s) 105, 826, 1457
Bsh1236I CGCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 972, 1340
BsiHKAI GWGCWC 3 cut(s) 907, 1396, 1432
BsiSI CCGG 1 cut(s) 854
BslI CCNNNNNNNGG 3 cut(s) 141, 600, 1751
BsmAI GTCTC 3 cut(s) 25, 557, 1543
BsnI GGCC 2 cut(s) 972, 1340
Bso31I GGTCTC 1 cut(s) 1543
Bsp1286I GDGCHC 3 cut(s) 907, 1396, 1432
Bsp1407I TGTACA 2 cut(s) 201, 1005
Bsp143I GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
Bsp19I CCATGG 1 cut(s) 1108
BspACI CCGC 6 cut(s) 266, 387, 765, 812, 957, 1128
BspANI GGCC 2 cut(s) 972, 1340
BspCNI CTCAG 3 cut(s) 408, 955, 1558
BspFNI CGCG 1 cut(s) 216
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 161, 1106
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 833
BspPI GGATC 2 cut(s) 845, 1414
BspQI GCTCTTC 1 cut(s) 1401
BspTI CTTAAG 1 cut(s) 1186
BspTNI GGTCTC 1 cut(s) 1543
BsrDI GCAATG 1 cut(s) 777
BsrGI TGTACA 2 cut(s) 201, 1005
BsrI ACTGG 2 cut(s) 31, 345
BssECI CCNNGG 6 cut(s) 88, 613, 854, 1108, 1341, 1598
BssMI GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
BssT1I CCWWGG 4 cut(s) 88, 613, 1108, 1341
Bst2UI CCWGG 2 cut(s) 466, 1600
Bst4CI ACNGT 1 cut(s) 1766
Bst6I CTCTTC 1 cut(s) 1401
BstAFI CTTAAG 1 cut(s) 1186
BstAPI GCANNNNNTGC 1 cut(s) 762
BstAUI TGTACA 2 cut(s) 201, 1005
BstC8I GCNNGC 3 cut(s) 6, 626, 837
BstDEI CTNAG 5 cut(s) 395, 869, 963, 1545, 1697
BstDSI CCRYGG 1 cut(s) 1108
BstF5I GGATG 4 cut(s) 349, 460, 1581, 1594
BstFNI CGCG 1 cut(s) 216
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 216
BstKTI GATC 7 cut(s) 523, 853, 1144, 1288, 1309, 1322, 1409
BstMAI GTCTC 3 cut(s) 25, 557, 1543
BstMBI GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
BstMWI GCNNNNNNNGC 1 cut(s) 762
BstNI CCWGG 2 cut(s) 466, 1600
BstNSI RCATGY 2 cut(s) 8, 1006
BstSCI CCNGG 3 cut(s) 464, 853, 1598
BstSFI CTRYAG 2 cut(s) 573, 840
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 3 cut(s) 105, 826, 1457
BstV2I GAAGAC 2 cut(s) 432, 644
BstX2I RGATCY 3 cut(s) 520, 1319, 1406
BstXI CCANNNNNNTGG 1 cut(s) 1115
BstYI RGATCY 3 cut(s) 520, 1319, 1406
BsuRI GGCC 2 cut(s) 972, 1340
BtgI CCRYGG 1 cut(s) 1108
BtsCI GGATG 4 cut(s) 349, 460, 1581, 1594
BtsI GCAGTG 1 cut(s) 1708
BtsIMutI CAGTG 2 cut(s) 804, 1708
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 3 cut(s) 6, 626, 837
CciI TCATGA 1 cut(s) 655
CfoI GCGC 2 cut(s) 98, 216
Cfr13I GGNCC 1 cut(s) 159
Csp6I GTAC 3 cut(s) 202, 408, 1006
CviQI GTAC 3 cut(s) 202, 408, 1006
DdeI CTNAG 5 cut(s) 395, 869, 963, 1545, 1697
DpnI GATC 7 cut(s) 522, 852, 1143, 1287, 1308, 1321, 1408
DpnII GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
Eam1104I CTCTTC 1 cut(s) 1401
EarI CTCTTC 1 cut(s) 1401
Eco130I CCWWGG 4 cut(s) 88, 613, 1108, 1341
Eco147I AGGCCT 2 cut(s) 972, 1340
Eco31I GGTCTC 1 cut(s) 1543
Eco47I GGWCC 1 cut(s) 159
Eco47III AGCGCT 1 cut(s) 97
Eco57I CTGAAG 3 cut(s) 828, 929, 1248
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 2 cut(s) 464, 1598
EcoT14I CCWWGG 4 cut(s) 88, 613, 1108, 1341
EcoT22I ATGCAT 2 cut(s) 6, 889
ErhI CCWWGG 4 cut(s) 88, 613, 1108, 1341
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FblI GTMKAC 1 cut(s) 1630
Fnu4HI GCNGC 5 cut(s) 94, 388, 840, 1128, 1446
FokI GGATG 4 cut(s) 356, 467, 1581, 1588
Fsp4HI GCNGC 5 cut(s) 94, 388, 840, 1128, 1446
FspBI CTAG 4 cut(s) 17, 1035, 1560, 1686
GlaI GCGC 2 cut(s) 97, 215
GluI GCNGC 5 cut(s) 94, 388, 840, 1128, 1446
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 2 cut(s) 972, 1340
HapII CCGG 1 cut(s) 854
HhaI GCGC 2 cut(s) 98, 216
Hin6I GCGC 2 cut(s) 96, 214
HinP1I GCGC 2 cut(s) 96, 214
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 3 cut(s) 622, 1189, 1239
HinfI GANTC 5 cut(s) 145, 170, 185, 604, 783
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 1 cut(s) 854
HphI GGTGA 4 cut(s) 296, 629, 1171, 1498
Hpy166II GTNNAC 2 cut(s) 259, 1631
Hpy188I TCNGA 4 cut(s) 721, 909, 1548, 1762
Hpy8I GTNNAC 2 cut(s) 259, 1631
HpyAV CCTTC 5 cut(s) 962, 1151, 1192, 1223, 1528
HpyCH4III ACNGT 1 cut(s) 1766
HpyCH4IV ACGT 1 cut(s) 1633
HpyF10VI GCNNNNNNNGC 1 cut(s) 762
HpyF3I CTNAG 5 cut(s) 395, 869, 963, 1545, 1697
HpySE526I ACGT 1 cut(s) 1633
HspAI GCGC 2 cut(s) 96, 214
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
LguI GCTCTTC 1 cut(s) 1401
LmnI GCTCC 1 cut(s) 1104
Lsp1109I GCAGC 3 cut(s) 105, 826, 1457
LweI GCATC 3 cut(s) 201, 525, 874
MaeI CTAG 4 cut(s) 17, 1035, 1560, 1686
MaeII ACGT 1 cut(s) 1633
MaeIII GTNAC 4 cut(s) 398, 1177, 1222, 1668
MalI GATC 7 cut(s) 522, 852, 1143, 1287, 1308, 1321, 1408
MboI GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
MboII GAAGA 4 cut(s) 428, 437, 644, 1388
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 3 cut(s) 520, 1319, 1406
MhlI GDGCHC 3 cut(s) 907, 1396, 1432
MlyI GAGTC 1 cut(s) 777
MmeI TCCRAC 2 cut(s) 663, 796
Mph1103I ATGCAT 2 cut(s) 6, 889
MseI TTAA 7 cut(s) 258, 569, 998, 1047, 1187, 1523, 1710
MslI CAYNNNNRTG 2 cut(s) 660, 1574
MspCI CTTAAG 1 cut(s) 1186
MspI CCGG 1 cut(s) 854
MspR9I CCNGG 3 cut(s) 466, 855, 1600
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 2 cut(s) 466, 1600
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 762
NciI CCSGG 1 cut(s) 855
NcoI CCATGG 1 cut(s) 1108
NdeII GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
NlaIV GGNNCC 2 cut(s) 161, 1106
NmuCI GTSAC 2 cut(s) 1177, 1222
NsiI ATGCAT 2 cut(s) 6, 889
NspI RCATGY 2 cut(s) 8, 1006
PaeI GCATGC 1 cut(s) 8
PagI TCATGA 1 cut(s) 655
PceI AGGCCT 2 cut(s) 972, 1340
PciI ACATGT 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 1401
PfeI GAWTC 4 cut(s) 145, 170, 185, 604
PflMI CCANNNNNTGG 1 cut(s) 141
PfoI TCCNGGA 1 cut(s) 464
PkrI GCNGC 5 cut(s) 95, 389, 841, 1129, 1447
PleI GAGTC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 777
PpuMI RGGWCCY 1 cut(s) 159
PscI ACATGT 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 464, 1598
PspGI CCWGG 2 cut(s) 464, 1598
PspN4I GGNNCC 2 cut(s) 161, 1106
PspPI GGNCC 1 cut(s) 159
PspPPI RGGWCCY 1 cut(s) 159
PsrI GAACNNNNNNTAC 4 cut(s) 62, 94, 1461, 1493
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 3 cut(s) 520, 1319, 1406
RsaI GTAC 3 cut(s) 203, 409, 1007
RsaNI GTAC 3 cut(s) 202, 408, 1006
RseI CAYNNNNRTG 2 cut(s) 660, 1574
SapI GCTCTTC 1 cut(s) 1401
SaqAI TTAA 7 cut(s) 258, 569, 998, 1047, 1187, 1523, 1710
SatI GCNGC 5 cut(s) 94, 388, 840, 1128, 1446
Sau3AI GATC 7 cut(s) 520, 850, 1141, 1285, 1306, 1319, 1406
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 1 cut(s) 777
ScrFI CCNGG 3 cut(s) 466, 855, 1600
SduI GDGCHC 3 cut(s) 907, 1396, 1432
SfaNI GCATC 3 cut(s) 201, 525, 874
SfcI CTRYAG 2 cut(s) 573, 840
SinI GGWCC 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 660, 1574
SmlI CTYRAG 1 cut(s) 1186
SmoI CTYRAG 1 cut(s) 1186
SphI GCATGC 1 cut(s) 8
SseBI AGGCCT 2 cut(s) 972, 1340
SsiI CCGC 6 cut(s) 266, 387, 765, 812, 957, 1128
SspI AATATT 1 cut(s) 472
SspMI CTAG 4 cut(s) 17, 1035, 1560, 1686
StuI AGGCCT 2 cut(s) 972, 1340
StyD4I CCNGG 3 cut(s) 464, 853, 1598
StyI CCWWGG 4 cut(s) 88, 613, 1108, 1341
TaaI ACNGT 1 cut(s) 1766
TaiI ACGT 1 cut(s) 1636
TaqI TCGA 2 cut(s) 423, 494
TatI WGTACW 2 cut(s) 201, 1005
TauI GCSGC 2 cut(s) 390, 1130
TfiI GAWTC 4 cut(s) 145, 170, 185, 604
Tru1I TTAA 7 cut(s) 258, 569, 998, 1047, 1187, 1523, 1710
Tru9I TTAA 7 cut(s) 258, 569, 998, 1047, 1187, 1523, 1710
TscAI CASTG 2 cut(s) 811, 1708
TseFI GTSAC 2 cut(s) 1177, 1222
TseI GCWGC 3 cut(s) 93, 839, 1445
Tsp45I GTSAC 2 cut(s) 1177, 1222
TspDTI ATGAA 7 cut(s) 299, 471, 644, 779, 917, 1268, 1558
TspRI CASTG 2 cut(s) 811, 1708
Van91I CCANNNNNTGG 1 cut(s) 141
Vha464I CTTAAG 1 cut(s) 1186
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 3 cut(s) 895, 1413, 1565
XbaI TCTAGA 1 cut(s) 1034
XceI RCATGY 2 cut(s) 8, 1006
XmiI GTMKAC 1 cut(s) 1630
XspI CTAG 4 cut(s) 17, 1035, 1560, 1686
Zsp2I ATGCAT 2 cut(s) 6, 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.