Rh2CG297200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0012639
Physical Location & Seq
Reverse (-)
34253666 .. 34257375
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG297200.1

Sequence Viewer

Length: 1788 bp
ATGCGCGAGTTGGGTGTTTCACATGATTGTTTTACATTTCCAATTGTTAACAAGGCGGTCTTGTTGGTTGGGAGTGATGTTAGATATGCAGGAATGGTTCATTCTGTGGCAATTCAAATGGGTTTTGGACTGGATGTGTATTTTGGCAATACTATGATTGAGGTGTATGTGAAATGCGGCAATCTCAGTTACGCCCGTATGTTGTTTGATGAAATGCCTGACACAGATTTAGTTTCTTGGACTTCAATGATTTCCGGGTATGTTTCTGAAGGAAATGTTGCCTCTGGGTTTAGTTTGTTCAGTGAAATGAGGATGGAACTGGAACCGAATTCGGTGACAATGTTGGTCATGTTGCAAGCGTGTTGTGGTTTTCAAACTTCAATATACGGGAGGCAAGTTCATGGATTTGTGATTAAGAATGGTTTGCTAAGTAATGGAGCTATTCAGAACTCAATCTTGAGAATGTATGCTAAATTAGGTACCATTGAAGAAGTCGAAGACTTTTTCAGAGAACTTGATAGGAGGGATGTTGTTTCCTGGAATATTTGTATTTCATCTTATACTTCGAGAGGGGATTTTGTGAAAGTAAGAGATTTGTTCAATGAGATGCAGGGTGGAGTAGCACCAAGTATTGAGACATTAACTATAGTTCTGTCAGCATTGACCAAACATGGGATTCTATCCCAAGGTGAAAGCTTGCATGGTTTAGCAATAAAAAGTGGTCTTCATGATGATGTTTTGCAGACATCTTTGTTGGACTTTTATGCCAAATGTGGAAAATTGGAAAGCTCGGATAAATTGTTTAGAGAACTCCCTGATAGAAACTGCATTACTTGCGGTGCAATGATGTTAGGACTCATTCATAATGGGTATTTCAATGAAGCGGTTGGAGTATTTAGACAGATGCAAGCTGCAGGTCTTGATCCAGGGGCAGAGATACTTAGAAACCTTATTGATGCATTTGCAAATTTAGGTGCTCTGAAGTTGGGAAAAGCAGTTCATGGCTACATAATAAGGAAATCATTTTGCGGAACTGAGGAAGGCCATACTCATCTTGAAACCTCCATCTTAAACATGTACATAAGGTGTGGTAGCATATCTACATCTAGAGTGTGTTTTAATAGAATGGTTTTCAAAGATGTGGTGGCATGGACATCAATGATTGAGGGTTATGGGAGCCATGGGCTTGGTTTTGAAGCCGCAAAACTCTTTGATCTAATGACAAGAGAAGGAATAAAGCCAAATAGTGTCACCTTCTTAAGCTTGTTATCTGCTTATAGCCACTCTGGTCTTGTGACTGAAGGCTGTGAAGCTTTTTATTACATGAAATGGAGGTTTGGAATTGAACCCGATATAGATCATTACACTTGTGTTGTTGATCTCTTGGGCAGATCTGGAAAGCTCAAAGAGGCCTTGGCTGTCATTTTGAAAATGTTGGCTTTTCCAGATAGTAGGATTTGGGGTGCTCTTCTTTCAGGATCTAGAATTTACGGCAACAGAGCACTTGGGCAATATGCAGCCCAAAGGCTCTTGGAATTAGAACCTGGTAATGTTGGATATTTTACTCTGTTGAGTAATACAGAAGCCAGTGTTGGACATTGGGATGAAGTAGAAGAGATAAGGAAAGTTATGAAAGAGAATGACCTAAAGAAGAAGCCAGGGTGGAGCTGCATTGAGGCCAATGGAGTGATTCATGGGTTTGTTTCTGGAGGTAATTTGCACCATCACATTGAGGAGATTTATGAAGTACTGGGGTGGTTAAGTAGGATGATGCAAGGGTTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

595

Amino Acids

66.04

Weight (kDa)

5.86

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 76 - 122 3.5e-10 PPR repeat family
PPR PF01535 78 - 105 3.5e-06 PPR repeat
PPR_2 PF13041 175 - 222 1.1e-08 PPR repeat family
PPR_2 PF13041 379 - 427 1.8e-11 PPR repeat family
PPR PF01535 382 - 412 4.8e-07 PPR repeat
E_motif PF20431 497 - 559 8.9e-18 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24950
malus_domestica MD05G1013600.v1.1 MD14G1083400.v1.1
prunus_persica Prupe.7G047100_v2.0.a1
pyrus_communis pycom14g07240
rosa_chinensis RchiOBHm_Chr2g0124611
rosa_laevigata RLG00000018808
rosa_multiflora Rmu_sc0005597.1_g000009
rosa_roxburghii Rroxscaffold_2G00119840
rosa_rugosa Rorug02G0251600 Rorug02G0251700
rosa_samantha Rh2AG310300 Rh2AG310400 Rh2BG318800 Rh2CG297200 Rh2CG302100 Rh2DG334000
rosa_wichuraiana Rw0G009400 Rw2G025100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 905
Acc65I GGTACC 1 cut(s) 479
AccB1I GGYRCC 1 cut(s) 479
AccII CGCG 1 cut(s) 6
AciI CCGC 6 cut(s) 56, 177, 837, 884, 1029, 1200
AclWI GGATC 2 cut(s) 917, 1486
AcsI RAATTY 3 cut(s) 328, 967, 1485
AcuI CTGAAG 3 cut(s) 288, 1001, 1320
AfaI GTAC 3 cut(s) 481, 1079, 1749
AfiI CCNNNNNNNGG 1 cut(s) 672
AflII CTTAAG 1 cut(s) 1258
AflIII ACRYGT 1 cut(s) 1074
AjnI CCWGG 4 cut(s) 536, 925, 1543, 1657
AjuI GAANNNNNNNTTGG 2 cut(s) 1575, 1607
AloI GAACNNNNNNTCC 2 cut(s) 981, 1013
AluBI AGCT 8 cut(s) 440, 696, 789, 911, 1263, 1313, 1402, 1668
AluI AGCT 8 cut(s) 440, 696, 789, 911, 1263, 1313, 1402, 1668
Alw21I GWGCWC 3 cut(s) 979, 1468, 1504
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 2 cut(s) 917, 1486
AoxI GGCC 3 cut(s) 1042, 1410, 1677
ApeKI GCWGC 3 cut(s) 911, 1517, 1668
ApoI RAATTY 3 cut(s) 328, 967, 1485
Asp718I GGTACC 1 cut(s) 479
AspLEI GCGC 1 cut(s) 6
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 3 cut(s) 346, 701, 1243
BanI GGYRCC 1 cut(s) 479
BbsI GAAGAC 2 cut(s) 504, 716
Bbv12I GWGCWC 3 cut(s) 979, 1468, 1504
BbvI GCAGC 3 cut(s) 898, 1529, 1655
BccI CCATC 3 cut(s) 307, 1073, 1731
BceAI ACGGC 1 cut(s) 1507
BciT130I CCWGG 4 cut(s) 538, 927, 1545, 1659
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 2 cut(s) 1107, 1482
BfmI CTRYAG 2 cut(s) 645, 912
BfrI CTTAAG 1 cut(s) 1258
BfuAI ACCTGC 1 cut(s) 905
BglII AGATCT 1 cut(s) 1391
BisI GCNGC 5 cut(s) 178, 912, 1200, 1518, 1669
BlsI GCNGC 5 cut(s) 179, 913, 1201, 1519, 1670
BmcAI AGTACT 1 cut(s) 1749
Bme1390I CCNGG 5 cut(s) 256, 538, 927, 1545, 1659
BmiI GGNNCC 3 cut(s) 324, 481, 1178
BmrFI CCNGG 5 cut(s) 256, 538, 927, 1545, 1659
BmrI ACTGGG 1 cut(s) 1760
BmsI GCATC 4 cut(s) 597, 894, 946, 1761
BmuI ACTGGG 1 cut(s) 1760
BpiI GAAGAC 2 cut(s) 504, 716
BpmI CTGGAG 1 cut(s) 1728
BpuEI CTTGAG 1 cut(s) 478
BpuMI CCSGG 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 1356
BsaJI CCNNGG 5 cut(s) 685, 926, 1180, 1413, 1658
Bsc4I CCNNNNNNNGG 1 cut(s) 672
Bse1I ACTGG 4 cut(s) 135, 324, 1587, 1755
Bse3DI GCAATG 1 cut(s) 849
Bse8I GATNNNNATC 1 cut(s) 1356
BseBI CCWGG 4 cut(s) 538, 927, 1545, 1659
BseDI CCNNGG 5 cut(s) 685, 926, 1180, 1413, 1658
BseGI GGATG 5 cut(s) 139, 318, 532, 1609, 1773
BseJI GATNNNNATC 1 cut(s) 1356
BseLI CCNNNNNNNGG 1 cut(s) 672
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 2 cut(s) 199, 1026
BseNI ACTGG 4 cut(s) 135, 324, 1587, 1755
BseRI GAGGAG 1 cut(s) 1748
BseXI GCAGC 3 cut(s) 898, 1529, 1655
Bsh1236I CGCG 1 cut(s) 6
BshFI GGCC 3 cut(s) 1044, 1412, 1679
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 3 cut(s) 979, 1468, 1504
BsiSI CCGG 1 cut(s) 255
BslI CCNNNNNNNGG 1 cut(s) 672
BsmAI GTCTC 1 cut(s) 629
BsnI GGCC 3 cut(s) 1044, 1412, 1679
Bsp1286I GDGCHC 3 cut(s) 979, 1468, 1504
Bsp1407I TGTACA 1 cut(s) 1077
Bsp143I GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
Bsp19I CCATGG 1 cut(s) 1180
BspACI CCGC 6 cut(s) 56, 177, 837, 884, 1029, 1200
BspANI GGCC 3 cut(s) 1044, 1412, 1679
BspCNI CTCAG 2 cut(s) 198, 1027
BspFNI CGCG 1 cut(s) 6
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 3 cut(s) 324, 481, 1178
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 1 cut(s) 905
BspPI GGATC 2 cut(s) 917, 1486
BspQI GCTCTTC 1 cut(s) 1473
BspT107I GGYRCC 1 cut(s) 479
BspTI CTTAAG 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 849
BsrGI TGTACA 1 cut(s) 1077
BsrI ACTGG 4 cut(s) 135, 324, 1587, 1755
BssECI CCNNGG 5 cut(s) 685, 926, 1180, 1413, 1658
BssMI GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
BssT1I CCWWGG 3 cut(s) 685, 1180, 1413
Bst2UI CCWGG 4 cut(s) 538, 927, 1545, 1659
Bst6I CTCTTC 2 cut(s) 1473, 1608
BstAFI CTTAAG 1 cut(s) 1258
BstAPI GCANNNNNTGC 1 cut(s) 834
BstAUI TGTACA 1 cut(s) 1077
BstC8I GCNNGC 3 cut(s) 357, 698, 909
BstDEI CTNAG 4 cut(s) 185, 428, 941, 1035
BstDSI CCRYGG 1 cut(s) 1180
BstF5I GGATG 5 cut(s) 139, 318, 532, 1609, 1773
BstFNI CGCG 1 cut(s) 6
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 6 cut(s) 925, 1216, 1360, 1381, 1394, 1481
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
BstMWI GCNNNNNNNGC 1 cut(s) 834
BstNI CCWGG 4 cut(s) 538, 927, 1545, 1659
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 5 cut(s) 254, 536, 925, 1543, 1657
BstSFI CTRYAG 2 cut(s) 645, 912
BstUI CGCG 1 cut(s) 6
BstV1I GCAGC 3 cut(s) 898, 1529, 1655
BstV2I GAAGAC 2 cut(s) 504, 716
BstX2I RGATCY 2 cut(s) 1391, 1478
BstXI CCANNNNNNTGG 1 cut(s) 1187
BstYI RGATCY 2 cut(s) 1391, 1478
BsuRI GGCC 3 cut(s) 1044, 1412, 1679
BtgI CCRYGG 1 cut(s) 1180
BtsCI GGATG 5 cut(s) 139, 318, 532, 1609, 1773
BtsIMutI CAGTG 2 cut(s) 307, 1594
BveI ACCTGC 1 cut(s) 905
Cac8I GCNNGC 3 cut(s) 357, 698, 909
CciI TCATGA 1 cut(s) 727
CfoI GCGC 1 cut(s) 6
CsiI ACCWGGT 1 cut(s) 1543
Csp6I GTAC 3 cut(s) 480, 1078, 1748
CviQI GTAC 3 cut(s) 480, 1078, 1748
DdeI CTNAG 4 cut(s) 185, 428, 941, 1035
DpnI GATC 6 cut(s) 924, 1215, 1359, 1380, 1393, 1480
DpnII GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
Eam1104I CTCTTC 2 cut(s) 1473, 1608
EarI CTCTTC 2 cut(s) 1473, 1608
Eco130I CCWWGG 3 cut(s) 685, 1180, 1413
Eco147I AGGCCT 1 cut(s) 1412
Eco57I CTGAAG 3 cut(s) 288, 1001, 1320
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 4 cut(s) 536, 925, 1543, 1657
EcoT14I CCWWGG 3 cut(s) 685, 1180, 1413
EcoT22I ATGCAT 1 cut(s) 961
ErhI CCWWGG 3 cut(s) 685, 1180, 1413
FalI AAGNNNNNCTT 2 cut(s) 44, 76
Fnu4HI GCNGC 5 cut(s) 178, 912, 1200, 1518, 1669
FokI GGATG 5 cut(s) 146, 325, 539, 1616, 1780
Fsp4HI GCNGC 5 cut(s) 178, 912, 1200, 1518, 1669
FspBI CTAG 2 cut(s) 1107, 1482
GlaI GCGC 1 cut(s) 5
GluI GCNGC 5 cut(s) 178, 912, 1200, 1518, 1669
GsuI CTGGAG 1 cut(s) 1728
HaeIII GGCC 3 cut(s) 1044, 1412, 1679
HapII CCGG 1 cut(s) 255
HhaI GCGC 1 cut(s) 6
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 3 cut(s) 694, 1261, 1311
HinfI GANTC 3 cut(s) 676, 855, 1690
HpaI GTTAAC 1 cut(s) 49
HpaII CCGG 1 cut(s) 255
HphI GGTGA 3 cut(s) 346, 701, 1243
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 5 cut(s) 268, 447, 509, 793, 981
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 5 cut(s) 263, 1034, 1223, 1264, 1295
HpyF10VI GCNNNNNNNGC 1 cut(s) 834
HpyF3I CTNAG 4 cut(s) 185, 428, 941, 1035
HspAI GCGC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 483
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
LguI GCTCTTC 1 cut(s) 1473
LmnI GCTCC 3 cut(s) 437, 1176, 1665
Lsp1109I GCAGC 3 cut(s) 898, 1529, 1655
LweI GCATC 4 cut(s) 597, 894, 946, 1761
MabI ACCWGGT 1 cut(s) 1543
MaeI CTAG 2 cut(s) 1107, 1482
MaeIII GTNAC 4 cut(s) 188, 334, 1249, 1294
MalI GATC 6 cut(s) 924, 1215, 1359, 1380, 1393, 1480
MboI GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
MboII GAAGA 6 cut(s) 500, 509, 716, 1460, 1625, 1663
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 2 cut(s) 1391, 1478
MhlI GDGCHC 3 cut(s) 979, 1468, 1504
MlyI GAGTC 1 cut(s) 849
MmeI TCCRAC 4 cut(s) 735, 868, 1534, 1573
Mph1103I ATGCAT 1 cut(s) 961
MseI TTAA 7 cut(s) 48, 414, 641, 1070, 1119, 1259, 1760
MslI CAYNNNNRTG 2 cut(s) 732, 1602
MspCI CTTAAG 1 cut(s) 1258
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 5 cut(s) 256, 538, 927, 1545, 1659
MunI CAATTG 1 cut(s) 42
MvaI CCWGG 4 cut(s) 538, 927, 1545, 1659
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 1 cut(s) 834
NciI CCSGG 1 cut(s) 256
NcoI CCATGG 1 cut(s) 1180
NdeII GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
NlaIV GGNNCC 3 cut(s) 324, 481, 1178
NmuCI GTSAC 3 cut(s) 334, 1249, 1294
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 1 cut(s) 1078
PagI TCATGA 1 cut(s) 727
PceI AGGCCT 1 cut(s) 1412
PciI ACATGT 1 cut(s) 1074
PciSI GCTCTTC 1 cut(s) 1473
PfeI GAWTC 2 cut(s) 676, 1690
PfoI TCCNGGA 1 cut(s) 536
PkrI GCNGC 5 cut(s) 179, 913, 1201, 1519, 1670
PleI GAGTC 1 cut(s) 849
PpsI GAGTC 1 cut(s) 849
PscI ACATGT 1 cut(s) 1074
Psp6I CCWGG 4 cut(s) 536, 925, 1543, 1657
PspGI CCWGG 4 cut(s) 536, 925, 1543, 1657
PspN4I GGNNCC 3 cut(s) 324, 481, 1178
PstI CTGCAG 1 cut(s) 916
PsuI RGATCY 2 cut(s) 1391, 1478
RsaI GTAC 3 cut(s) 481, 1079, 1749
RsaNI GTAC 3 cut(s) 480, 1078, 1748
RseI CAYNNNNRTG 2 cut(s) 732, 1602
SapI GCTCTTC 1 cut(s) 1473
SaqAI TTAA 7 cut(s) 48, 414, 641, 1070, 1119, 1259, 1760
SatI GCNGC 5 cut(s) 178, 912, 1200, 1518, 1669
Sau3AI GATC 6 cut(s) 922, 1213, 1357, 1378, 1391, 1478
ScaI AGTACT 1 cut(s) 1749
SchI GAGTC 1 cut(s) 849
ScrFI CCNGG 5 cut(s) 256, 538, 927, 1545, 1659
SduI GDGCHC 3 cut(s) 979, 1468, 1504
SexAI ACCWGGT 1 cut(s) 1543
SfaNI GCATC 4 cut(s) 597, 894, 946, 1761
SfcI CTRYAG 2 cut(s) 645, 912
SmiMI CAYNNNNRTG 2 cut(s) 732, 1602
SmlI CTYRAG 2 cut(s) 457, 1258
SmoI CTYRAG 2 cut(s) 457, 1258
SseBI AGGCCT 1 cut(s) 1412
SsiI CCGC 6 cut(s) 56, 177, 837, 884, 1029, 1200
SspI AATATT 1 cut(s) 544
SspMI CTAG 2 cut(s) 1107, 1482
StuI AGGCCT 1 cut(s) 1412
StyD4I CCNGG 5 cut(s) 254, 536, 925, 1543, 1657
StyI CCWWGG 3 cut(s) 685, 1180, 1413
TaqI TCGA 2 cut(s) 495, 566
TatI WGTACW 2 cut(s) 1077, 1747
TauI GCSGC 2 cut(s) 180, 1202
TfiI GAWTC 2 cut(s) 676, 1690
Tru1I TTAA 7 cut(s) 48, 414, 641, 1070, 1119, 1259, 1760
Tru9I TTAA 7 cut(s) 48, 414, 641, 1070, 1119, 1259, 1760
TscAI CASTG 2 cut(s) 307, 1594
TseFI GTSAC 3 cut(s) 334, 1249, 1294
TseI GCWGC 3 cut(s) 911, 1517, 1668
Tsp45I GTSAC 3 cut(s) 334, 1249, 1294
TspRI CASTG 2 cut(s) 307, 1594
Vha464I CTTAAG 1 cut(s) 1258
XapI RAATTY 3 cut(s) 328, 967, 1485
XbaI TCTAGA 2 cut(s) 1106, 1481
XceI RCATGY 1 cut(s) 1078
XspI CTAG 2 cut(s) 1107, 1482
ZrmI AGTACT 1 cut(s) 1749
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.