MD14G1084700.v1.1

protein disulfide oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016640
Physical Location & Seq
Reverse (-)
10304609 .. 10305823
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1084700.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGGGTGCTCTGCTTCAAGACCCCTCACCCTTCTAACCAAAAACCCAGAACAGAATTCACAAATTGCTCCTACTTCCTTCCAAGACTCTTCCAATTCCTCCCCACTTCTCTCCACCTCCAGAAGAGCTCTCTCAATCTCCTCTGCCCCTCTCGTCCACCACCCTCCTCTCCGAAAAGGCGACACTCACCACCTCGTCTCCCTCACCTCCACCACCTACGGCTCCCTCCGTCTCGACCCTCCATCTCCCTTAAACCCCCTCCTCAATCTCAACGCTCAGGATTCTCCTGAACATCACCCCCACCCCCCAAAACACTCCACCTCCTCCCCCGACTCCGTCATCAACACCTGGGAACTCATGGATGGTCTCAACGACTCCGACTTCCACACCCATTTCTCTGCAAAACACCCCATTTCTGACCGCCCCATCGCTTTTCCCGAGAAGGCCAGTTCTTGCAGGTACTCAAGAAGACCGTTTGATTCTTTCGAATCGGCGAAATACTCGGAAGTAAAGGTAGAGGACGCATTGCCAGCCCTTTCATCTTCTTCGTCGAATTCAAAGCCTCTGTGGCAGCATTTAGCCGAGGAATCGTTGTTGTCGAAAATGGACCCCAATGTGGTCTTGACTTACAGAAGAGCATTGTCCTCTAAACAGTTGGGCGACAGCAGCAACAACCGATGCAATGTTGTAAGATCATTCACTTGTAGTAGTACACCTTTGCTACATTCCCCATTTTCAAATGCTTGTTTTTGTCTTCCGGGCGCTGAGGATAAAATCGTTATCTACTTCACCACATTGAGAGGAATTCGAAAGACTTACGAGGATTGTTGCGCCGTTCGGATGATATTTAGGGGATTTAGAGTGGCGGTAGACGAGAGGGACGTTTCAATGGACTCGGAGTACAGGAAAGAGTTGCAAAATGCGGTTGGGGACAAGGCGGTGAGTCTGCCACAAGTGTTTGTTAGAGGCAAGTATGTTGGAGGCGCAGAGGAGGTTAAGCAACTCAACGAGGCCGGAGAATTGGGGAAGCTTTTGAGGGGGTTTCCGGTGAGGGATGGCGGGTTTGTTTGTGAAGGATGTGGGGATGCAAGGTTTGCACCGTGCCCGACATGTAATGGGAGCCGAAAGCTGTTTGATGAGGATGAAGGGGAACTGAGGAGATGCCAGGATTGCAACGAAAACGGGTTGATTAGGTGCCCTGCTTGCTGCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

405

Amino Acids

44.52

Weight (kDa)

6.88

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 260 - 326 5.6e-13 Glutaredoxin
GRXCR1-2_C PF23733 357 - 403 5.7e-20 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016640)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41330 AT3G57070
malus_domestica MD14G1084700.v1.1
prunus_persica Prupe.7G043200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0125081
rosa_laevigata RLG00000018848
rosa_multiflora Rmu_sc0003505.1_g000023 Rmu_sc0003505.1_g000024
rosa_roxburghii Rroxscaffold_2G00118710
rosa_rugosa Rorug02G0255600
rosa_samantha Rh2AG314000 Rh2BG322300 Rh2DG340700
rosa_wichuraiana Rw2G025410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 1194
AccI GTMKAC 1 cut(s) 870
AciI CCGC 5 cut(s) 421, 866, 923, 938, 1059
AcsI RAATTY 3 cut(s) 55, 553, 804
AfaI GTAC 3 cut(s) 461, 712, 902
AfiI CCNNNNNNNGG 1 cut(s) 616
AflIII ACRYGT 1 cut(s) 1109
AgsI TTSAA 4 cut(s) 18, 558, 738, 888
AjnI CCWGG 2 cut(s) 347, 1164
AluBI AGCT 3 cut(s) 128, 1030, 1129
AluI AGCT 3 cut(s) 128, 1030, 1129
Alw21I GWGCWC 2 cut(s) 11, 130
Alw26I GTCTC 3 cut(s) 202, 236, 371
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 2 cut(s) 444, 1011
ApeKI GCWGC 3 cut(s) 571, 666, 1206
ApoI RAATTY 3 cut(s) 55, 553, 804
AspLEI GCGC 3 cut(s) 764, 833, 986
AspS9I GGNCC 1 cut(s) 607
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 7 cut(s) 19, 179, 196, 287, 781, 952, 1060
AsuII TTCGAA 2 cut(s) 486, 808
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 607
BaeGI GKGCMC 2 cut(s) 1106, 1199
BanI GGYRCC 1 cut(s) 1194
BanII GRGCYC 1 cut(s) 130
BbsI GAAGAC 2 cut(s) 475, 746
Bbv12I GWGCWC 2 cut(s) 11, 130
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 3 cut(s) 583, 678, 1193
BccI CCATC 4 cut(s) 250, 356, 434, 1049
BceAI ACGGC 2 cut(s) 235, 818
BciT130I CCWGG 2 cut(s) 349, 1166
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 3 cut(s) 202, 236, 371
BfoI RGCGCY 1 cut(s) 765
BfuAI ACCTGC 1 cut(s) 447
BglI GCCNNNNNGGC 1 cut(s) 568
BisI GCNGC 3 cut(s) 572, 667, 1207
BlsI GCNGC 3 cut(s) 573, 668, 1208
Bme1390I CCNGG 3 cut(s) 349, 759, 1166
Bme18I GGWCC 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 607
BmiI GGNNCC 4 cut(s) 223, 609, 1121, 1196
BmrFI CCNGG 3 cut(s) 349, 759, 1166
BmsI GCATC 3 cut(s) 668, 1075, 1151
BpiI GAAGAC 2 cut(s) 475, 746
BpmI CTGGAG 1 cut(s) 103
Bpu10I CCTNAGC 2 cut(s) 276, 765
Bpu14I TTCGAA 2 cut(s) 486, 808
BpuEI CTTGAG 1 cut(s) 448
BpuMI CCSGG 1 cut(s) 759
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 348, 582
BsaWI WCCGGW 1 cut(s) 1045
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse1I ACTGG 1 cut(s) 447
Bse3DI GCAATG 2 cut(s) 524, 688
BseBI CCWGG 2 cut(s) 349, 1166
BseDI CCNNGG 2 cut(s) 348, 582
BseGI GGATG 6 cut(s) 367, 846, 1060, 1082, 1090, 1147
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 2 cut(s) 524, 688
BseMII CTCAG 3 cut(s) 290, 756, 1145
BseNI ACTGG 1 cut(s) 447
BseRI GAGGAG 6 cut(s) 130, 156, 251, 313, 1004, 1171
BseSI GKGCMC 2 cut(s) 1106, 1199
BseXI GCAGC 3 cut(s) 583, 678, 1193
BshFI GGCC 2 cut(s) 446, 1013
BshNI GGYRCC 1 cut(s) 1194
BsiHKAI GWGCWC 2 cut(s) 11, 130
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 3 cut(s) 758, 1014, 1046
BslFI GGGAC 2 cut(s) 893, 944
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 3 cut(s) 202, 236, 371
BsmBI CGTCTC 2 cut(s) 202, 236
BsmFI GGGAC 2 cut(s) 893, 944
BsnI GGCC 2 cut(s) 446, 1013
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 2 cut(s) 486, 808
Bsp1286I GDGCHC 4 cut(s) 11, 130, 1106, 1199
Bsp143I GATC 1 cut(s) 692
BspACI CCGC 5 cut(s) 421, 866, 923, 938, 1059
BspANI GGCC 2 cut(s) 446, 1013
BspCNI CTCAG 3 cut(s) 289, 757, 1146
BspLI GGNNCC 4 cut(s) 223, 609, 1121, 1196
BspMI ACCTGC 1 cut(s) 447
BspQI GCTCTTC 2 cut(s) 118, 628
BspT104I TTCGAA 2 cut(s) 486, 808
BspT107I GGYRCC 1 cut(s) 1194
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 2 cut(s) 524, 688
BsrI ACTGG 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 348, 582
BssMI GATC 1 cut(s) 692
Bst2UI CCWGG 2 cut(s) 349, 1166
Bst4CI ACNGT 3 cut(s) 474, 654, 1101
Bst6I CTCTTC 3 cut(s) 94, 118, 628
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBI TTCGAA 2 cut(s) 486, 808
BstC8I GCNNGC 2 cut(s) 531, 1204
BstDEI CTNAG 3 cut(s) 276, 765, 1154
BstF5I GGATG 6 cut(s) 367, 846, 1060, 1082, 1090, 1147
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 3 cut(s) 764, 833, 986
BstKTI GATC 1 cut(s) 695
BstMAI GTCTC 3 cut(s) 202, 236, 371
BstMBI GATC 1 cut(s) 692
BstMWI GCNNNNNNNGC 6 cut(s) 530, 568, 666, 1094, 1170, 1203
BstNI CCWGG 2 cut(s) 349, 1166
BstNSI RCATGY 1 cut(s) 1113
BstSCI CCNGG 3 cut(s) 347, 757, 1164
BstSLI GKGCMC 2 cut(s) 1106, 1199
BstV1I GCAGC 3 cut(s) 583, 678, 1193
BstV2I GAAGAC 2 cut(s) 475, 746
BsuRI GGCC 2 cut(s) 446, 1013
BtgZI GCGATG 1 cut(s) 412
BtsCI GGATG 6 cut(s) 367, 846, 1060, 1082, 1090, 1147
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 2 cut(s) 531, 1204
CfoI GCGC 3 cut(s) 764, 833, 986
Cfr13I GGNCC 1 cut(s) 607
CseI GACGC 1 cut(s) 530
Csp6I GTAC 3 cut(s) 460, 711, 901
CviAII CATG 2 cut(s) 358, 1110
CviQI GTAC 3 cut(s) 460, 711, 901
DdeI CTNAG 3 cut(s) 276, 765, 1154
DpnI GATC 1 cut(s) 694
DpnII GATC 1 cut(s) 692
Eam1104I CTCTTC 3 cut(s) 94, 118, 628
EarI CTCTTC 3 cut(s) 94, 118, 628
Ecl136II GAGCTC 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 130
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 607
Eco53kI GAGCTC 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 128
EcoRI GAATTC 3 cut(s) 55, 553, 804
EcoRII CCWGG 2 cut(s) 347, 1164
EcoT38I GRGCYC 1 cut(s) 130
Esp3I CGTCTC 2 cut(s) 202, 236
FaeI CATG 2 cut(s) 361, 1113
FaiI YATR 3 cut(s) 359, 975, 1111
FaqI GGGAC 2 cut(s) 893, 944
FatI CATG 2 cut(s) 357, 1109
FauI CCCGC 1 cut(s) 1052
FblI GTMKAC 1 cut(s) 870
Fnu4HI GCNGC 3 cut(s) 572, 667, 1207
FokI GGATG 6 cut(s) 374, 853, 1067, 1089, 1097, 1154
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 572, 667, 1207
GlaI GCGC 3 cut(s) 763, 832, 985
GluI GCNGC 3 cut(s) 572, 667, 1207
GsuI CTGGAG 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 765
HaeIII GGCC 2 cut(s) 446, 1013
HapII CCGG 3 cut(s) 758, 1014, 1046
HgaI GACGC 1 cut(s) 530
HhaI GCGC 3 cut(s) 764, 833, 986
Hin1II CATG 2 cut(s) 361, 1113
Hin6I GCGC 3 cut(s) 762, 831, 984
HinP1I GCGC 3 cut(s) 762, 831, 984
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 9 cut(s) 86, 281, 332, 374, 479, 488, 587, 893, 943
HpaII CCGG 3 cut(s) 758, 1014, 1046
HphI GGTGA 7 cut(s) 19, 179, 196, 287, 781, 952, 1060
Hpy166II GTNNAC 3 cut(s) 157, 713, 871
Hpy188I TCNGA 6 cut(s) 173, 379, 418, 505, 840, 898
Hpy188III TCNNGA 8 cut(s) 18, 120, 233, 278, 287, 437, 465, 622
Hpy8I GTNNAC 3 cut(s) 157, 713, 871
Hpy99I CGWCG 1 cut(s) 553
HpyAV CCTTC 5 cut(s) 41, 88, 436, 1067, 1139
HpyCH4III ACNGT 3 cut(s) 474, 654, 1101
HpyCH4IV ACGT 1 cut(s) 882
HpyCH4V TGCA 7 cut(s) 401, 456, 681, 916, 1088, 1097, 1173
HpyF10VI GCNNNNNNNGC 6 cut(s) 530, 568, 666, 1094, 1170, 1203
HpyF3I CTNAG 3 cut(s) 276, 765, 1154
HpySE526I ACGT 1 cut(s) 882
Hsp92II CATG 2 cut(s) 361, 1113
HspAI GCGC 3 cut(s) 762, 831, 984
Kzo9I GATC 1 cut(s) 692
LguI GCTCTTC 2 cut(s) 118, 628
LmnI GCTCC 3 cut(s) 73, 227, 1119
Lsp1109I GCAGC 3 cut(s) 583, 678, 1193
LweI GCATC 3 cut(s) 668, 1075, 1151
MaeII ACGT 1 cut(s) 882
MalI GATC 1 cut(s) 694
MboI GATC 1 cut(s) 692
MboII GAAGA 7 cut(s) 81, 135, 480, 534, 537, 645, 746
MhlI GDGCHC 4 cut(s) 11, 130, 1106, 1199
MluCI AATT 6 cut(s) 55, 63, 94, 553, 804, 1019
MlyI GAGTC 5 cut(s) 80, 326, 368, 887, 952
MmeI TCCRAC 2 cut(s) 402, 958
MseI TTAA 2 cut(s) 251, 996
MspI CCGG 3 cut(s) 758, 1014, 1046
MspR9I CCNGG 3 cut(s) 349, 759, 1166
MvaI CCWGG 2 cut(s) 349, 1166
MwoI GCNNNNNNNGC 6 cut(s) 530, 568, 666, 1094, 1170, 1203
NciI CCSGG 1 cut(s) 759
NdeII GATC 1 cut(s) 692
NlaIII CATG 2 cut(s) 361, 1113
NlaIV GGNNCC 4 cut(s) 223, 609, 1121, 1196
NmeAIII GCCGAG 1 cut(s) 607
NspI RCATGY 1 cut(s) 1113
NspV TTCGAA 2 cut(s) 486, 808
PciI ACATGT 1 cut(s) 1109
PciSI GCTCTTC 2 cut(s) 118, 628
PcsI WCGNNNNNNNCGW 3 cut(s) 435, 596, 879
PfeI GAWTC 4 cut(s) 281, 479, 488, 587
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 3 cut(s) 573, 668, 1208
PleI GAGTC 5 cut(s) 80, 326, 368, 887, 951
PpsI GAGTC 5 cut(s) 80, 326, 368, 887, 951
PscI ACATGT 1 cut(s) 1109
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 347, 1164
PspGI CCWGG 2 cut(s) 347, 1164
PspN4I GGNNCC 4 cut(s) 223, 609, 1121, 1196
PspPI GGNCC 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 335
RsaI GTAC 3 cut(s) 461, 712, 902
RsaNI GTAC 3 cut(s) 460, 711, 901
SacI GAGCTC 1 cut(s) 130
SapI GCTCTTC 2 cut(s) 118, 628
SaqAI TTAA 2 cut(s) 251, 996
SatI GCNGC 3 cut(s) 572, 667, 1207
Sau3AI GATC 1 cut(s) 692
Sau96I GGNCC 1 cut(s) 607
SchI GAGTC 5 cut(s) 80, 326, 368, 887, 952
ScrFI CCNGG 3 cut(s) 349, 759, 1166
SduI GDGCHC 4 cut(s) 11, 130, 1106, 1199
SfaNI GCATC 3 cut(s) 668, 1075, 1151
SfuI TTCGAA 2 cut(s) 486, 808
SinI GGWCC 1 cut(s) 607
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
Sse9I AATT 6 cut(s) 55, 63, 94, 553, 804, 1019
SsiI CCGC 5 cut(s) 421, 866, 923, 938, 1059
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 3 cut(s) 347, 757, 1164
TaaI ACNGT 3 cut(s) 474, 654, 1101
TaiI ACGT 1 cut(s) 885
TaqI TCGA 5 cut(s) 234, 486, 551, 599, 808
TasI AATT 6 cut(s) 55, 63, 94, 553, 804, 1019
TatI WGTACW 2 cut(s) 710, 900
TfiI GAWTC 4 cut(s) 281, 479, 488, 587
Tru1I TTAA 2 cut(s) 251, 996
Tru9I TTAA 2 cut(s) 251, 996
TseI GCWGC 3 cut(s) 571, 666, 1206
TspDTI ATGAA 2 cut(s) 528, 1158
TspGWI ACGGA 2 cut(s) 218, 325
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 607
XapI RAATTY 3 cut(s) 55, 553, 804
XceI RCATGY 1 cut(s) 1113
XmiI GTMKAC 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.