MD14G1123300.v1.1

KAT8 regulatory NSL complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007737
Physical Location & Seq
Reverse (-)
19774056 .. 19776481
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1123300.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGCGTCGCCACCACAGTCAAAACGCAGGCGAAAGAACCAGAGCAAAGACGAAGCAGATGACCCGTCAGCGGCACCTTCATCCACACCAGGGAAATTATCCCCTGTGGTGGTCTTTGCTCATGGTGCTGGTGCTCCTTCTTCGTCCGACTGGATGATCAGGTGGAAGGATATGTTGGGAAAAGCACTAAATGCTGTAGAAGTTGTTACCTTCGATTACCCATATATTTCTGGTGGGAAAAAGAGGGCTCCTCCAAAGGCTGAAAAGTTGGTCGATTTTCATGCTGATGTTGTCAAAAAGGCGGTTGCTAAGAACCCAGGTCATCCGTTGATTTTAGCCGGGAAATCTATGGGTTCAAGAGTCAGCTGCATGGTAGCTTGCAAGGAAGATATACATGCCTCAACAATAGTTTGCTTAGGATACCCACTAAAGGGCATGAATGGAGCAGTGCGAGATGAAACGATATTGGAACTTTCTATTCCCATAATGTTTGTACAGGGTAGTAAAGATGGGCTTTGTCCATTGGAAAAGCTAGAAACTACTCGCAAGAAGATTAAATGCCTTAGTGATTTGCATGTCATTGATGGTGGCGACCACTCCTTCAAGATTGGAAAGAAGCATCTGCAAACCACTGGGTTATCCCAAGACAAAGCTGAAGATCTTGCTCTTCAGGCCATTGCAGCTTTTTGGTTGAGGTCTCTTGAAGGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_5 Abhydrolase_6 DLH

Protein Analysis

238

Amino Acids

25.6

Weight (kDa)

9.3

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 35 - 140 2.6e-08 alpha/beta hydrolase fold
Abhydrolase_11 PF20408 36 - 228 9.4e-41 Alpha/beta hydrolase domain
Abhydrolase_6 PF12697 37 - 143 4.5e-07 Alpha/beta hydrolase family
Abhydrolase_5 PF12695 93 - 200 5e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AciI CCGC 2 cut(s) 71, 302
AcuI CTGAAG 2 cut(s) 653, 675
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 5 cut(s) 70, 90, 109, 430, 431
AgsI TTSAA 3 cut(s) 357, 604, 704
AjnI CCWGG 2 cut(s) 88, 316
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 5 cut(s) 366, 377, 532, 653, 683
AluI AGCT 5 cut(s) 366, 377, 532, 653, 683
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 702
AoxI GGCC 1 cut(s) 672
ApeKI GCWGC 2 cut(s) 366, 680
AsuC2I CCSGG 1 cut(s) 340
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 2 cut(s) 353, 692
BccI CCATC 2 cut(s) 503, 578
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 2 cut(s) 90, 318
BciVI GTATCC 1 cut(s) 413
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 702
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 1 cut(s) 195
BfuI GTATCC 1 cut(s) 413
BglII AGATCT 1 cut(s) 658
BisI GCNGC 3 cut(s) 72, 367, 681
BlsI GCNGC 3 cut(s) 73, 368, 682
Bme1390I CCNGG 3 cut(s) 90, 318, 340
BmiI GGNNCC 2 cut(s) 75, 249
BmrFI CCNGG 3 cut(s) 90, 318, 340
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 1 cut(s) 628
BmuI ACTGGG 1 cut(s) 642
BplI GAGNNNNNCTC 2 cut(s) 235, 267
Bpu10I CCTNAGC 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 340
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 2 cut(s) 89, 316
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 90, 109, 430, 431
Bse1I ACTGG 2 cut(s) 155, 637
Bse3DI GCAATG 1 cut(s) 675
BseBI CCWGG 2 cut(s) 90, 318
BseDI CCNNGG 2 cut(s) 89, 316
BseGI GGATG 3 cut(s) 80, 159, 322
BseLI CCNNNNNNNGG 5 cut(s) 70, 90, 109, 430, 431
BseMI GCAATG 1 cut(s) 675
BseNI ACTGG 2 cut(s) 155, 637
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 2 cut(s) 353, 692
BshFI GGCC 1 cut(s) 674
BshNI GGYRCC 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 1 cut(s) 339
BslI CCNNNNNNNGG 5 cut(s) 70, 90, 109, 430, 431
BsmAI GTCTC 1 cut(s) 702
BsnI GGCC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 136, 250
Bsp1407I TGTACA 1 cut(s) 493
Bsp143I GATC 2 cut(s) 156, 658
BspACI CCGC 2 cut(s) 71, 302
BspANI GGCC 1 cut(s) 674
BspLI GGNNCC 2 cut(s) 75, 249
BspQI GCTCTTC 1 cut(s) 672
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 702
BsrDI GCAATG 1 cut(s) 675
BsrGI TGTACA 1 cut(s) 493
BsrI ACTGG 2 cut(s) 155, 637
BssECI CCNNGG 2 cut(s) 89, 316
BssMI GATC 2 cut(s) 156, 658
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 90, 318
Bst4CI ACNGT 1 cut(s) 18
Bst6I CTCTTC 1 cut(s) 672
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 493
BstC8I GCNNGC 2 cut(s) 29, 379
BstDEI CTNAG 3 cut(s) 309, 415, 563
BstF5I GGATG 3 cut(s) 80, 159, 322
BstKTI GATC 2 cut(s) 159, 661
BstMAI GTCTC 1 cut(s) 702
BstMBI GATC 2 cut(s) 156, 658
BstMWI GCNNNNNNNGC 4 cut(s) 125, 191, 671, 680
BstNI CCWGG 2 cut(s) 90, 318
BstNSI RCATGY 2 cut(s) 398, 578
BstSCI CCNGG 3 cut(s) 88, 316, 338
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 353, 692
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 1 cut(s) 674
BtsCI GGATG 3 cut(s) 80, 159, 322
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 453, 630
Cac8I GCNNGC 2 cut(s) 29, 379
Csp6I GTAC 1 cut(s) 494
CviAII CATG 6 cut(s) 122, 281, 370, 395, 436, 575
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 3 cut(s) 309, 415, 563
DpnI GATC 2 cut(s) 158, 660
DpnII GATC 2 cut(s) 156, 658
Eam1104I CTCTTC 1 cut(s) 672
EarI CTCTTC 1 cut(s) 672
Eco24I GRGCYC 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 702
Eco57I CTGAAG 2 cut(s) 653, 675
EcoRII CCWGG 2 cut(s) 88, 316
EcoT38I GRGCYC 1 cut(s) 250
FaeI CATG 6 cut(s) 125, 284, 373, 398, 439, 578
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FatI CATG 6 cut(s) 121, 280, 369, 394, 435, 574
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 72, 367, 681
FokI GGATG 3 cut(s) 67, 166, 309
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 3 cut(s) 72, 367, 681
FspBI CTAG 1 cut(s) 533
GluI GCNGC 3 cut(s) 72, 367, 681
HaeIII GGCC 1 cut(s) 674
HapII CCGG 1 cut(s) 339
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 125, 284, 373, 398, 439, 578
HinfI GANTC 1 cut(s) 360
HpaII CCGG 1 cut(s) 339
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 357, 604, 701
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 7 cut(s) 87, 147, 160, 220, 610, 698, 702
HpyCH4III ACNGT 1 cut(s) 18
HpyCH4V TGCA 5 cut(s) 369, 381, 574, 625, 680
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 191, 671, 680
HpyF3I CTNAG 3 cut(s) 309, 415, 563
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 125, 284, 373, 398, 439, 578
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 2 cut(s) 156, 658
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 3 cut(s) 139, 253, 443
Lsp1109I GCAGC 2 cut(s) 353, 692
LweI GCATC 1 cut(s) 628
MaeI CTAG 1 cut(s) 533
MaeIII GTNAC 1 cut(s) 205
MalI GATC 2 cut(s) 158, 660
MboI GATC 2 cut(s) 156, 658
MboII GAAGA 5 cut(s) 132, 398, 562, 659, 668
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 2 cut(s) 136, 250
MluCI AATT 1 cut(s) 95
MlyI GAGTC 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 4 cut(s) 237, 261, 409, 687
MseI TTAA 1 cut(s) 555
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 71, 366
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 3 cut(s) 90, 318, 340
MvaI CCWGG 2 cut(s) 90, 318
MwoI GCNNNNNNNGC 4 cut(s) 125, 191, 671, 680
NciI CCSGG 1 cut(s) 340
NdeII GATC 2 cut(s) 156, 658
NlaIII CATG 6 cut(s) 125, 284, 373, 398, 439, 578
NlaIV GGNNCC 2 cut(s) 75, 249
NspI RCATGY 2 cut(s) 398, 578
PciSI GCTCTTC 1 cut(s) 672
PkrI GCNGC 3 cut(s) 73, 368, 682
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 88, 316
PspGI CCWGG 2 cut(s) 88, 316
PspN4I GGNNCC 2 cut(s) 75, 249
PsuI RGATCY 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 366
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 672
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 3 cut(s) 72, 367, 681
Sau3AI GATC 2 cut(s) 156, 658
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 3 cut(s) 90, 318, 340
SduI GDGCHC 2 cut(s) 136, 250
SfaNI GCATC 1 cut(s) 628
SfcI CTRYAG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 285
Sse9I AATT 1 cut(s) 95
SsiI CCGC 2 cut(s) 71, 302
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 3 cut(s) 88, 316, 338
TaaI ACNGT 1 cut(s) 18
TaqI TCGA 2 cut(s) 213, 273
TasI AATT 1 cut(s) 95
TatI WGTACW 1 cut(s) 493
TauI GCSGC 1 cut(s) 74
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 2 cut(s) 453, 637
TseI GCWGC 2 cut(s) 366, 680
TspDTI ATGAA 4 cut(s) 69, 269, 452, 471
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 2 cut(s) 453, 637
XceI RCATGY 2 cut(s) 398, 578
XspI CTAG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.