MD14G1164000.v1.1

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006640
Physical Location & Seq
Reverse (-)
25793323 .. 25799346
6024 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1164000.v1.1.491

Sequence Viewer

Length: 1995 bp
ATGGGGAAGAAGACGAAGAAGCCGGGAAAGGGCAAAGAGAAGACGGAGAAGAAAACGGCGAAAGCTGAGGAGAAGAGAGCTCGCAGAGAGACCAAGAAGCTCTCTCCCGAGGATGACATCGATGCCATTTTGTCAAGTATTCAAAAGGAAGAAGCTAAGAAGAAGGAAGTTCATGTCGAGGAAAATGTCCCAGCTCCCTCTCCGCGGTCCAATTGCACACTGAGCGTCAATCCCTTGAAAGAGACAGAGTTGATCCTTTATGGGGGTGAATTCTATAATGGTAATAAGACATTTGTGTATGGTGATCTTTACCGGTATGATGTTGAAAAGCAGGACTGGAAGTTAATTTCGAGCCCCAATAGTCCACCTCCTCGTAGTGCCCACCAGGCGGTCACTTGGAAGAACTATCTCTACATCTTTGGTGGTGAGTTTACTTCCCCAAACCAAGAGAGGTTCCATCACTACAAGGACTTTTGGATGTTGGACCTCAAAACAAATCAATGGGAACAACTAAATATAAAGGGGAGTCCAAGTCCACGCTCAGGTCATCGGATGGTACTGTACAAGCACAAGATTATTGTTTTTGGAGGCTTCTATGACACGCTTAGAGAAGTGAGGTATCACAATGATCTCTTTGTATTTGACCTGGATCAGTTCAAGTGGCAAGAAATAACTCCTAGGCCTGGTTCCTTGTGGCCAAGCGCCCGAAGTGGTTTTCAGTTTTTTGTGTACCAAGATGAAATTTTCTTATATGGTGGCTATTCCAAAGAAGTTTCATCTGATAAAACTGGCTCCGAGAAAGGAATTGTTCATTCAGACTTGTGGGCCCTTGATCCTAGGACTTGGGAATGGAACAAGGTGAAGAAAAGTGGGATGCCTCCTGGACCTCGTGCTGGGTTTTCTATGTGTGATGTAATGATGAGCTTGTTCTTGGACGAACTGTATGGCTTCCAATTAGACAACCATCGGTGGTACCCATTAGAGCTACGGAAGGCGAAGTCAACAAAAGATAAGAGTTCTGGACGAAAGCGTAAAAATGATGATAAGATAAACTCAATTGAACCAGAGGAATGTGCAACGAATGAAAAAGATGTGAACTCTGAGAATGATGAAGCAGCAGATGACTTCGAACGCAAAGTTGACGGTATATCGCATCAAATGGCTAAAACCATTACTGTTGGTAATGATCGGTTAGCTGACAAATCTGATGGAAAGATACATGAATCTACTGCTAATATGGATACACAAGACAAATCTGATGGAAAGATACATGAATCTACTGCTAATATGGATACACAAGATTATGATTTGCCAGAGGTAGTGAAGCCCTGTGGACGTATCAATTCTTGCATGGTTGTTGGAAGAGATACATTATATGTTTACGGGGGAATGATGGAGGTTAAAGATCGAGAAATTACACTCGATGATTTGTATTCTCTAAATCTTAGCAAACTTGACGAGTGGAAGTGCATCATACCGGCAACAGAAACTGAATGGGTGGAGGCTTCCGAGGATGAAGATGAGGATGAAGATGACGATGATGATAGTGAAGATGAAGGTGATGAAGATAGTAACAGTGATGAGACCGATGATGATAATGATGAAGAGAATGGGAAAGATGGATGGATTCAGATGGGGGATGCTGTTGTTTTAATCAAGGGTGAGGGAAAGAGTCTCCGTAGGAAAGAGAAACGGGCCAGAATTGAGCAGATCAGAGCCAGTCTTGGACTTTCAGATTCGCAAAGAACCCCAACGCCTGGTGAAACTTTGAGAGAATTCTACAAACGTACAAATATGTACTGGCAAATGGCGGCACACGAACATACTCAACACACTGGAAAGGAGCTCCGCAAGGATGGTTTTGATCTTGCTGAAGCGCGATACAGAGAGCTTAAACCAATTCTCGATGAGTTGGCCATATTAGAGGCAGAACAGAAGGCTGTGGAGCAAGAACCGGAAACTAGTACCAAAAAAAGAGGCAAGAAGAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

665

Amino Acids

76.52

Weight (kDa)

5.24

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 55 - 253 8.1e-41 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 57 - 198 5.4e-28 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 86 - 172 7.9e-15 Attractin/LZTR1 beta-propeller
NANM PF24996 103 - 224 1.5e-09 N-acetylneuraminate epimerase
Kelch_FKB95 PF25210 110 - 264 4.3e-08 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 154 - 305 9.2e-29 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 154 - 227 3.2e-14 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 179 - 226 5.4e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 208 - 302 1.7e-10 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 264 - 330 2.3e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
DUF4110 PF13422 576 - 662 2.3e-29 Domain of unknown function (DUF4110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 972
AccB1I GGYRCC 1 cut(s) 972
AccB7I CCANNNNNTGG 1 cut(s) 1757
AccII CGCG 2 cut(s) 205, 1879
AciI CCGC 5 cut(s) 203, 205, 389, 1811, 1849
AclWI GGATC 3 cut(s) 247, 657, 827
AcoI YGGCCR 2 cut(s) 695, 1914
AcsI RAATTY 3 cut(s) 269, 741, 1775
AcuI CTGAAG 1 cut(s) 1893
AfaI GTAC 7 cut(s) 558, 563, 731, 974, 1789, 1799, 1966
AfiI CCNNNNNNNGG 8 cut(s) 29, 204, 262, 388, 542, 683, 893, 1757
AgeI ACCGGT 1 cut(s) 312
AgsI TTSAA 5 cut(s) 143, 238, 326, 658, 1061
AhlI ACTAGT 1 cut(s) 1961
AjnI CCWGG 5 cut(s) 384, 645, 682, 880, 1756
Alw21I GWGCWC 2 cut(s) 82, 1848
Alw26I GTCTC 4 cut(s) 83, 236, 1577, 1679
AlwI GGATC 3 cut(s) 247, 657, 827
AlwNI CAGNNNCTG 1 cut(s) 1490
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 5 cut(s) 680, 695, 825, 1695, 1914
ApaI GGGCCC 1 cut(s) 829
ApeKI GCWGC 1 cut(s) 1115
ApoI RAATTY 3 cut(s) 269, 741, 1775
AsiGI ACCGGT 1 cut(s) 312
Asp718I GGTACC 1 cut(s) 972
AspA2I CCTAGG 2 cut(s) 677, 836
AspLEI GCGC 2 cut(s) 704, 1879
AspS9I GGNCC 6 cut(s) 207, 484, 825, 826, 884, 1695
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 7 cut(s) 278, 314, 437, 871, 1571, 1673, 1772
AsuII TTCGAA 1 cut(s) 1128
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 3 cut(s) 207, 484, 884
AvrII CCTAGG 2 cut(s) 677, 836
BaeGI GKGCMC 2 cut(s) 382, 829
BalI TGGCCA 2 cut(s) 697, 1916
BanI GGYRCC 1 cut(s) 972
BanII GRGCYC 4 cut(s) 82, 356, 829, 1848
BauI CACGAG 1 cut(s) 888
BbsI GAAGAC 2 cut(s) 17, 47
Bbv12I GWGCWC 2 cut(s) 82, 1848
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 1127
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 5 cut(s) 386, 647, 684, 882, 1758
BciVI GTATCC 2 cut(s) 1234, 1285
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 4 cut(s) 83, 236, 1577, 1679
BcuI ACTAGT 1 cut(s) 1961
BfaI CTAG 3 cut(s) 678, 837, 1962
BfoI RGCGCY 1 cut(s) 705
BfuI GTATCC 2 cut(s) 1234, 1285
BglI GCCNNNNNGGC 1 cut(s) 386
BisI GCNGC 2 cut(s) 1116, 1812
BlnI CCTAGG 2 cut(s) 677, 836
BlsI GCNGC 2 cut(s) 1117, 1813
Bme1390I CCNGG 6 cut(s) 24, 386, 647, 684, 882, 1758
Bme18I GGWCC 3 cut(s) 207, 484, 884
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 6 cut(s) 207, 484, 825, 826, 884, 1695
BmiI GGNNCC 5 cut(s) 455, 688, 793, 827, 974
BmrFI CCNGG 6 cut(s) 24, 386, 647, 684, 882, 1758
BmsI GCATC 5 cut(s) 112, 864, 1162, 1479, 1630
BpiI GAAGAC 2 cut(s) 17, 47
Bpu10I CCTNAGC 2 cut(s) 66, 541
Bpu14I TTCGAA 1 cut(s) 1128
BpuMI CCSGG 1 cut(s) 24
Bsa29I ATCGAT 1 cut(s) 120
BsaI GGTCTC 2 cut(s) 83, 1577
BsaJI CCNNGG 5 cut(s) 108, 203, 677, 836, 1509
BsaWI WCCGGW 2 cut(s) 312, 1954
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bsc4I CCNNNNNNNGG 8 cut(s) 29, 204, 262, 388, 542, 683, 893, 1757
Bse118I RCCGGY 2 cut(s) 312, 1477
Bse1I ACTGG 5 cut(s) 341, 793, 1719, 1805, 1840
BseBI CCWGG 5 cut(s) 386, 647, 684, 882, 1758
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 5 cut(s) 108, 203, 677, 836, 1509
BseGI GGATG 9 cut(s) 118, 483, 558, 879, 1519, 1531, 1628, 1645, 1861
BseLI CCNNNNNNNGG 8 cut(s) 29, 204, 262, 388, 542, 683, 893, 1757
BseMII CTCAG 4 cut(s) 57, 212, 555, 1092
BseNI ACTGG 5 cut(s) 341, 793, 1719, 1805, 1840
BseRI GAGGAG 2 cut(s) 83, 360
BseSI GKGCMC 2 cut(s) 382, 829
BseXI GCAGC 1 cut(s) 1127
BseYI CCCAGC 2 cut(s) 190, 893
Bsh1236I CGCG 2 cut(s) 205, 1879
BshFI GGCC 5 cut(s) 682, 697, 827, 1697, 1916
BshNI GGYRCC 1 cut(s) 972
BshTI ACCGGT 1 cut(s) 312
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 2 cut(s) 82, 1848
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 4 cut(s) 23, 313, 1478, 1955
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 8 cut(s) 29, 204, 262, 388, 542, 683, 893, 1757
BsmAI GTCTC 4 cut(s) 83, 236, 1577, 1679
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 5 cut(s) 682, 697, 827, 1697, 1916
Bso31I GGTCTC 2 cut(s) 83, 1577
BsoBI CYCGRG 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 1128
Bsp120I GGGCCC 1 cut(s) 825
Bsp1286I GDGCHC 5 cut(s) 82, 356, 382, 829, 1848
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
BspACI CCGC 5 cut(s) 203, 205, 389, 1811, 1849
BspANI GGCC 5 cut(s) 682, 697, 827, 1697, 1916
BspCNI CTCAG 4 cut(s) 58, 213, 554, 1093
BspDI ATCGAT 1 cut(s) 120
BspFNI CGCG 2 cut(s) 205, 1879
BspLI GGNNCC 5 cut(s) 455, 688, 793, 827, 974
BspPI GGATC 3 cut(s) 247, 657, 827
BspT104I TTCGAA 1 cut(s) 1128
BspT107I GGYRCC 1 cut(s) 972
BspTNI GGTCTC 2 cut(s) 83, 1577
BsrFI RCCGGY 2 cut(s) 312, 1477
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 5 cut(s) 341, 793, 1719, 1805, 1840
BssAI RCCGGY 2 cut(s) 312, 1477
BssECI CCNNGG 5 cut(s) 108, 203, 677, 836, 1509
BssMI GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
BssSI CACGAG 1 cut(s) 888
BssT1I CCWWGG 2 cut(s) 677, 836
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 5 cut(s) 386, 647, 684, 882, 1758
Bst4CI ACNGT 5 cut(s) 561, 942, 1145, 1177, 1577
Bst6I CTCTTC 3 cut(s) 68, 1357, 1599
BstAUI TGTACA 1 cut(s) 561
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 7 cut(s) 66, 156, 221, 541, 605, 1101, 1445
BstDSI CCRYGG 1 cut(s) 203
BstF5I GGATG 9 cut(s) 118, 483, 558, 879, 1519, 1531, 1628, 1645, 1861
BstFNI CGCG 2 cut(s) 205, 1879
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 2 cut(s) 704, 1879
BstKTI GATC 9 cut(s) 255, 307, 631, 652, 835, 1189, 1408, 1713, 1867
BstMAI GTCTC 4 cut(s) 83, 236, 1577, 1679
BstMBI GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
BstMWI GCNNNNNNNGC 2 cut(s) 222, 386
BstNI CCWGG 5 cut(s) 386, 647, 684, 882, 1758
BstSCI CCNGG 6 cut(s) 22, 384, 645, 682, 880, 1756
BstSLI GKGCMC 2 cut(s) 382, 829
BstUI CGCG 2 cut(s) 205, 1879
BstV1I GCAGC 1 cut(s) 1127
BstV2I GAAGAC 2 cut(s) 17, 47
Bsu15I ATCGAT 1 cut(s) 120
BsuI GTATCC 2 cut(s) 1234, 1285
BsuRI GGCC 5 cut(s) 682, 697, 827, 1697, 1916
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 203
BtsCI GGATG 9 cut(s) 118, 483, 558, 879, 1519, 1531, 1628, 1645, 1861
BtsIMutI CAGTG 3 cut(s) 218, 1582, 1833
Cac8I GCNNGC 1 cut(s) 82
CaiI CAGNNNCTG 1 cut(s) 1490
CfoI GCGC 2 cut(s) 704, 1879
Cfr10I RCCGGY 2 cut(s) 312, 1477
Cfr13I GGNCC 6 cut(s) 207, 484, 825, 826, 884, 1695
Cfr42I CCGCGG 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 1 cut(s) 214
Csp6I GTAC 7 cut(s) 557, 562, 730, 973, 1788, 1798, 1965
CspAI ACCGGT 1 cut(s) 312
CviAII CATG 4 cut(s) 173, 1220, 1271, 1351
CviQI GTAC 7 cut(s) 557, 562, 730, 973, 1788, 1798, 1965
DdeI CTNAG 7 cut(s) 66, 156, 221, 541, 605, 1101, 1445
DpnI GATC 9 cut(s) 254, 306, 630, 651, 834, 1188, 1407, 1712, 1866
DpnII GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
EaeI YGGCCR 2 cut(s) 695, 1914
Eam1104I CTCTTC 3 cut(s) 68, 1357, 1599
EarI CTCTTC 3 cut(s) 68, 1357, 1599
Ecl136II GAGCTC 2 cut(s) 80, 1846
Eco130I CCWWGG 2 cut(s) 677, 836
Eco147I AGGCCT 1 cut(s) 682
Eco24I GRGCYC 4 cut(s) 82, 356, 829, 1848
Eco31I GGTCTC 2 cut(s) 83, 1577
Eco47I GGWCC 3 cut(s) 207, 484, 884
Eco53kI GAGCTC 2 cut(s) 80, 1846
Eco57I CTGAAG 1 cut(s) 1893
Eco88I CYCGRG 1 cut(s) 107
EcoICRI GAGCTC 2 cut(s) 80, 1846
EcoO109I RGGNCCY 1 cut(s) 826
EcoRI GAATTC 2 cut(s) 269, 1775
EcoRII CCWGG 5 cut(s) 384, 645, 682, 880, 1756
EcoT14I CCWWGG 2 cut(s) 677, 836
EcoT38I GRGCYC 4 cut(s) 82, 356, 829, 1848
ErhI CCWWGG 2 cut(s) 677, 836
FaeI CATG 4 cut(s) 176, 1223, 1274, 1354
FaqI GGGAC 1 cut(s) 173
FatI CATG 4 cut(s) 172, 1219, 1270, 1350
Fnu4HI GCNGC 2 cut(s) 1116, 1812
FokI GGATG 9 cut(s) 125, 490, 565, 886, 1526, 1538, 1635, 1652, 1868
FriOI GRGCYC 4 cut(s) 82, 356, 829, 1848
Fsp4HI GCNGC 2 cut(s) 1116, 1812
FspBI CTAG 3 cut(s) 678, 837, 1962
GlaI GCGC 2 cut(s) 703, 1878
GluI GCNGC 2 cut(s) 1116, 1812
GsaI CCCAGC 2 cut(s) 194, 897
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 5 cut(s) 682, 697, 827, 1697, 1916
HapII CCGG 4 cut(s) 23, 313, 1478, 1955
HgaI GACGC 1 cut(s) 214
HhaI GCGC 2 cut(s) 704, 1879
Hin1II CATG 4 cut(s) 176, 1223, 1274, 1354
Hin6I GCGC 2 cut(s) 702, 1877
HinP1I GCGC 2 cut(s) 702, 1877
HincII GTYRAC 2 cut(s) 1002, 1141
HindII GTYRAC 2 cut(s) 1002, 1141
HinfI GANTC 6 cut(s) 526, 1223, 1274, 1627, 1672, 1736
HpaII CCGG 4 cut(s) 23, 313, 1478, 1955
HphI GGTGA 7 cut(s) 278, 314, 437, 871, 1571, 1673, 1772
Hpy166II GTNNAC 9 cut(s) 365, 432, 536, 730, 1002, 1096, 1141, 1334, 1381
Hpy188III TCNNGA 4 cut(s) 107, 1020, 1409, 1904
Hpy8I GTNNAC 9 cut(s) 365, 432, 536, 730, 1002, 1096, 1141, 1334, 1381
HpyAV CCTTC 4 cut(s) 157, 985, 1550, 1930
HpyCH4III ACNGT 5 cut(s) 561, 942, 1145, 1177, 1577
HpyCH4IV ACGT 2 cut(s) 1336, 1786
HpyCH4V TGCA 4 cut(s) 216, 1076, 1350, 1470
HpyF10VI GCNNNNNNNGC 2 cut(s) 222, 386
HpyF3I CTNAG 7 cut(s) 66, 156, 221, 541, 605, 1101, 1445
HpySE526I ACGT 2 cut(s) 1336, 1786
Hsp92II CATG 4 cut(s) 176, 1223, 1274, 1354
HspAI GCGC 2 cut(s) 702, 1877
KpnI GGTACC 1 cut(s) 976
KspI CCGCGG 1 cut(s) 206
Kzo9I GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
LmnI GCTCC 5 cut(s) 199, 797, 1843, 1851, 1945
Lsp1109I GCAGC 1 cut(s) 1127
LweI GCATC 5 cut(s) 112, 864, 1162, 1479, 1630
MaeI CTAG 3 cut(s) 678, 837, 1962
MaeII ACGT 2 cut(s) 1336, 1786
MaeIII GTNAC 2 cut(s) 391, 1571
MalI GATC 9 cut(s) 254, 306, 630, 651, 834, 1188, 1407, 1712, 1866
MboI GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
MfeI CAATTG 2 cut(s) 211, 1056
MhlI GDGCHC 5 cut(s) 82, 356, 382, 829, 1848
MlsI TGGCCA 2 cut(s) 697, 1916
MluNI TGGCCA 2 cut(s) 697, 1916
MlyI GAGTC 2 cut(s) 535, 1681
MmeI TCCRAC 2 cut(s) 462, 1339
Mox20I TGGCCA 2 cut(s) 697, 1916
MscI TGGCCA 2 cut(s) 697, 1916
MseI TTAA 4 cut(s) 344, 1401, 1652, 1893
Msp20I TGGCCA 2 cut(s) 697, 1916
MspA1I CMGCKG 1 cut(s) 205
MspI CCGG 4 cut(s) 23, 313, 1478, 1955
MspR9I CCNGG 6 cut(s) 24, 386, 647, 684, 882, 1758
MunI CAATTG 2 cut(s) 211, 1056
MvaI CCWGG 5 cut(s) 386, 647, 684, 882, 1758
MvnI CGCG 2 cut(s) 205, 1879
MwoI GCNNNNNNNGC 2 cut(s) 222, 386
NciI CCSGG 1 cut(s) 24
NdeII GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
NlaIII CATG 4 cut(s) 176, 1223, 1274, 1354
NlaIV GGNNCC 5 cut(s) 455, 688, 793, 827, 974
NmuCI GTSAC 1 cut(s) 391
NspV TTCGAA 1 cut(s) 1128
PceI AGGCCT 1 cut(s) 682
PfeI GAWTC 4 cut(s) 1223, 1274, 1627, 1736
PflMI CCANNNNNTGG 1 cut(s) 1757
PfoI TCCNGGA 1 cut(s) 880
PinAI ACCGGT 1 cut(s) 312
PkrI GCNGC 2 cut(s) 1117, 1813
PleI GAGTC 2 cut(s) 534, 1680
PpsI GAGTC 2 cut(s) 534, 1680
Psp124BI GAGCTC 2 cut(s) 82, 1848
Psp6I CCWGG 5 cut(s) 384, 645, 682, 880, 1756
PspFI CCCAGC 2 cut(s) 190, 893
PspGI CCWGG 5 cut(s) 384, 645, 682, 880, 1756
PspN4I GGNNCC 5 cut(s) 455, 688, 793, 827, 974
PspOMI GGGCCC 1 cut(s) 825
PspPI GGNCC 6 cut(s) 207, 484, 825, 826, 884, 1695
PsrI GAACNNNNNNTAC 2 cut(s) 395, 427
PstNI CAGNNNCTG 1 cut(s) 1490
RsaI GTAC 7 cut(s) 558, 563, 731, 974, 1789, 1799, 1966
RsaNI GTAC 7 cut(s) 557, 562, 730, 973, 1788, 1798, 1965
SacI GAGCTC 2 cut(s) 82, 1848
SacII CCGCGG 1 cut(s) 206
SaqAI TTAA 4 cut(s) 344, 1401, 1652, 1893
SatI GCNGC 2 cut(s) 1116, 1812
Sau3AI GATC 9 cut(s) 252, 304, 628, 649, 832, 1186, 1405, 1710, 1864
Sau96I GGNCC 6 cut(s) 207, 484, 825, 826, 884, 1695
SchI GAGTC 2 cut(s) 535, 1681
ScrFI CCNGG 6 cut(s) 24, 386, 647, 684, 882, 1758
SduI GDGCHC 5 cut(s) 82, 356, 382, 829, 1848
SfaNI GCATC 5 cut(s) 112, 864, 1162, 1479, 1630
Sfr303I CCGCGG 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 1128
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 3 cut(s) 207, 484, 884
SpeI ACTAGT 1 cut(s) 1961
SseBI AGGCCT 1 cut(s) 682
SsiI CCGC 5 cut(s) 203, 205, 389, 1811, 1849
SspMI CTAG 3 cut(s) 678, 837, 1962
SstI GAGCTC 2 cut(s) 82, 1848
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 6 cut(s) 22, 384, 645, 682, 880, 1756
StyI CCWWGG 2 cut(s) 677, 836
TaaI ACNGT 5 cut(s) 561, 942, 1145, 1177, 1577
TaiI ACGT 2 cut(s) 1339, 1789
TaqI TCGA 7 cut(s) 120, 177, 350, 1128, 1408, 1422, 1905
TaqII GACCGA 1 cut(s) 1601
TatI WGTACW 2 cut(s) 561, 1797
TauI GCSGC 1 cut(s) 1814
TfiI GAWTC 4 cut(s) 1223, 1274, 1627, 1736
Tru1I TTAA 4 cut(s) 344, 1401, 1652, 1893
Tru9I TTAA 4 cut(s) 344, 1401, 1652, 1893
TscAI CASTG 3 cut(s) 225, 1582, 1840
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 1 cut(s) 1115
Tsp45I GTSAC 1 cut(s) 391
TspGWI ACGGA 3 cut(s) 59, 1003, 1667
TspRI CASTG 3 cut(s) 225, 1582, 1840
Van91I CCANNNNNTGG 1 cut(s) 1757
VpaK11BI GGWCC 3 cut(s) 207, 484, 884
XapI RAATTY 3 cut(s) 269, 741, 1775
XmaJI CCTAGG 2 cut(s) 677, 836
XspI CTAG 3 cut(s) 678, 837, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.