Rw1G001960

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006640
Physical Location & Seq
Reverse (-)
3452500 .. 3453901
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G001960.1

Sequence Viewer

Length: 399 bp
ATGTTACAGGTATTGTACAAGCACAAGATTATTGTTTTTGGCGGCTTCTATGACACTCTAAGAGAAGTGAGGTATCATAATGATCTTTATGTATTTGACCTGGACCAGTTCAAGTGGCAAGAAATAACCCTTAGGCCTGGGGCCATGCGGCCAAGTGCACGAAGTGGCTTTCAATTTTTGTTTACCAAGATGAGATCTTTCTATATGGTGGCTATTCCAAAGAAGTTTCATCTGATAAAACTAGCTCTGAGAAAGGAATTGTTCATTCAGATTTATGGTCCCTTGATCCTAGGACATGGGAATGGAACAAGGTTAAAAAAAGTGGGATGCCTCCTGGACCCCGCGCTGGGTTTTCCATGTGTGTTCATAAGAAGAGAGCTTTGCTATTTGGGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

132

Amino Acids

15.52

Weight (kDa)

9.78

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 4 - 75 2.2e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 4 - 59 2.9e-06 FBX42, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 344
AciI CCGC 3 cut(s) 42, 148, 342
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 346, 347
AgsI TTSAA 2 cut(s) 112, 173
AjnI CCWGG 3 cut(s) 99, 136, 333
AluBI AGCT 2 cut(s) 245, 379
AluI AGCT 2 cut(s) 245, 379
Alw21I GWGCWC 1 cut(s) 160
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 1 cut(s) 280
AoxI GGCC 3 cut(s) 134, 141, 149
ApaLI GTGCAC 1 cut(s) 156
AspA2I CCTAGG 1 cut(s) 289
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 4 cut(s) 103, 141, 278, 337
AvaII GGWCC 3 cut(s) 103, 278, 337
AvrII CCTAGG 1 cut(s) 289
AxyI CCTNAGG 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 101, 138, 335
BfaI CTAG 2 cut(s) 242, 290
BglII AGATCT 1 cut(s) 194
BisI GCNGC 2 cut(s) 43, 149
BlnI CCTAGG 1 cut(s) 289
BlsI GCNGC 2 cut(s) 44, 150
Bme1390I CCNGG 3 cut(s) 101, 138, 335
Bme18I GGWCC 3 cut(s) 103, 278, 337
BmgT120I GGNCC 4 cut(s) 103, 141, 278, 337
BmiI GGNNCC 3 cut(s) 142, 280, 339
BmrFI CCNGG 3 cut(s) 101, 138, 335
BmsI GCATC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 137, 289
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 347
Bse1I ACTGG 1 cut(s) 106
Bse21I CCTNAGG 1 cut(s) 131
BseBI CCWGG 3 cut(s) 101, 138, 335
BseDI CCNNGG 2 cut(s) 137, 289
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 2 cut(s) 346, 347
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 106
BseSI GKGCMC 1 cut(s) 160
BseYI CCCAGC 1 cut(s) 346
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 136, 143, 151
BsiHKAI GWGCWC 1 cut(s) 160
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 346, 347
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 3 cut(s) 136, 143, 151
Bsp1286I GDGCHC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 3 cut(s) 82, 194, 285
BspACI CCGC 3 cut(s) 42, 148, 342
BspANI GGCC 3 cut(s) 136, 143, 151
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 3 cut(s) 142, 280, 339
BspPI GGATC 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 137, 289
BssMI GATC 3 cut(s) 82, 194, 285
BssT1I CCWWGG 1 cut(s) 289
Bst2UI CCWGG 3 cut(s) 101, 138, 335
Bst6I CTCTTC 1 cut(s) 367
BstAUI TGTACA 1 cut(s) 15
BstDEI CTNAG 3 cut(s) 59, 131, 248
BstF5I GGATG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 3 cut(s) 85, 197, 288
BstMBI GATC 3 cut(s) 82, 194, 285
BstNI CCWGG 3 cut(s) 101, 138, 335
BstSCI CCNGG 3 cut(s) 99, 136, 333
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 1 cut(s) 344
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
Bsu36I CCTNAGG 1 cut(s) 131
BsuRI GGCC 3 cut(s) 136, 143, 151
BtsCI GGATG 1 cut(s) 332
CfoI GCGC 1 cut(s) 346
Cfr13I GGNCC 4 cut(s) 103, 141, 278, 337
Csp6I GTAC 1 cut(s) 16
CviAII CATG 3 cut(s) 145, 296, 357
CviJI RGCY 8 cut(s) 45, 136, 143, 151, 168, 212, 245, 379
CviKI_1 RGCY 8 cut(s) 45, 136, 143, 151, 168, 212, 245, 379
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 59, 131, 248
DpnI GATC 3 cut(s) 84, 196, 287
DpnII GATC 3 cut(s) 82, 194, 285
DraIII CACNNNGTG 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 149
Eam1104I CTCTTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 136
Eco47I GGWCC 3 cut(s) 103, 278, 337
Eco81I CCTNAGG 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 99, 136, 333
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 3 cut(s) 148, 299, 360
FaqI GGGAC 1 cut(s) 264
FatI CATG 3 cut(s) 144, 295, 356
FauI CCCGC 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 43, 149
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 2 cut(s) 43, 149
FspBI CTAG 2 cut(s) 242, 290
GlaI GCGC 1 cut(s) 345
GluI GCNGC 2 cut(s) 43, 149
GsaI CCCAGC 1 cut(s) 350
HaeIII GGCC 3 cut(s) 136, 143, 151
HhaI GCGC 1 cut(s) 346
Hin1II CATG 3 cut(s) 148, 299, 360
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
Hpy166II GTNNAC 2 cut(s) 158, 183
Hpy188I TCNGA 3 cut(s) 234, 249, 270
Hpy8I GTNNAC 2 cut(s) 158, 183
HpyCH4V TGCA 1 cut(s) 158
HpyF3I CTNAG 3 cut(s) 59, 131, 248
Hsp92II CATG 3 cut(s) 148, 299, 360
HspAI GCGC 1 cut(s) 344
Kzo9I GATC 3 cut(s) 82, 194, 285
LpnPI CCDG 8 cut(s) 86, 113, 119, 123, 150, 320, 332, 347
LweI GCATC 1 cut(s) 317
MaeI CTAG 2 cut(s) 242, 290
MaeIII GTNAC 1 cut(s) 3
MalI GATC 3 cut(s) 84, 196, 287
MboI GATC 3 cut(s) 82, 194, 285
MboII GAAGA 1 cut(s) 384
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 2 cut(s) 173, 257
MnlI CCTC 2 cut(s) 63, 341
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 300
MspR9I CCNGG 3 cut(s) 101, 138, 335
MvaI CCWGG 3 cut(s) 101, 138, 335
MvnI CGCG 1 cut(s) 344
NdeII GATC 3 cut(s) 82, 194, 285
NlaIII CATG 3 cut(s) 148, 299, 360
NlaIV GGNNCC 3 cut(s) 142, 280, 339
PceI AGGCCT 1 cut(s) 136
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 2 cut(s) 44, 150
Psp6I CCWGG 3 cut(s) 99, 136, 333
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 3 cut(s) 99, 136, 333
PspN4I GGNNCC 3 cut(s) 142, 280, 339
PspPI GGNCC 4 cut(s) 103, 141, 278, 337
PsuI RGATCY 1 cut(s) 194
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 2 cut(s) 43, 149
Sau3AI GATC 3 cut(s) 82, 194, 285
Sau96I GGNCC 4 cut(s) 103, 141, 278, 337
ScrFI CCNGG 3 cut(s) 101, 138, 335
SduI GDGCHC 1 cut(s) 160
SetI ASST 6 cut(s) 12, 74, 102, 247, 314, 381
SfaNI GCATC 1 cut(s) 317
SinI GGWCC 3 cut(s) 103, 278, 337
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 2 cut(s) 173, 257
SseBI AGGCCT 1 cut(s) 136
SsiI CCGC 3 cut(s) 42, 148, 342
SspMI CTAG 2 cut(s) 242, 290
StuI AGGCCT 1 cut(s) 136
StyD4I CCNGG 3 cut(s) 99, 136, 333
StyI CCWWGG 1 cut(s) 289
TasI AATT 2 cut(s) 173, 257
TatI WGTACW 1 cut(s) 15
TauI GCSGC 2 cut(s) 45, 151
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TspDTI ATGAA 3 cut(s) 218, 253, 355
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 3 cut(s) 103, 278, 337
XmaJI CCTAGG 1 cut(s) 289
XspI CTAG 2 cut(s) 242, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.