Rh4DG264900

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006640
Physical Location & Seq
Forward (+)
48359573 .. 48362052
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG264900.1

Sequence Viewer

Length: 420 bp
ATGCATTATCGGTTATGTTACAGGTATTGTACAAGCACAAGATTATTAATTGTTTTTGGCGGCTTCTATGACACTCTAAGAGAAGTGAGGTATCATAATAATCTTTATGTATTTGACCTGGACCAGTTCAAGTGGCAAGAAATAACCCTTAGGCCTGGGGCCATGCGGCCAAGTGCACGAAGTGGCTTTCAATTTTTGTTTACCAAGATGAGATCTTTCTGTATGGTGGCTATTCCAAAGAAGTTTCATCTGATAAAACTGGCTCTGAGAAAGGAATTGTTCATTCAGATTTATGGTCCCTTGATCCTAGACATGGGAATGGAACAAGGTAAGAAAAAGTGGGATGCCTCCTGGACCTCGTACTGGGTTTTCTATATATGTGTGTTTATAAGAAGCGAGCTTTGCTATTTGGGGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

139

Amino Acids

16.78

Weight (kDa)

9.56

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 14 - 64 5.7e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AciI CCGC 2 cut(s) 60, 166
AclWI GGATC 1 cut(s) 298
AcoI YGGCCR 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 182
AfaI GTAC 2 cut(s) 31, 362
AfiI CCNNNNNNNGG 2 cut(s) 313, 363
AgsI TTSAA 2 cut(s) 130, 191
AjnI CCWGG 3 cut(s) 117, 154, 350
AluBI AGCT 1 cut(s) 400
AluI AGCT 1 cut(s) 400
Alw21I GWGCWC 1 cut(s) 178
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 1 cut(s) 298
AoxI GGCC 3 cut(s) 152, 159, 167
ApaLI GTGCAC 1 cut(s) 174
AseI ATTAAT 1 cut(s) 47
AspS9I GGNCC 4 cut(s) 121, 159, 296, 354
AvaII GGWCC 3 cut(s) 121, 296, 354
AxyI CCTNAGG 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 178
BciT130I CCWGG 3 cut(s) 119, 156, 352
BfaI CTAG 1 cut(s) 308
BglII AGATCT 1 cut(s) 212
BisI GCNGC 2 cut(s) 61, 167
BlsI GCNGC 2 cut(s) 62, 168
Bme1390I CCNGG 3 cut(s) 119, 156, 352
Bme18I GGWCC 3 cut(s) 121, 296, 354
BmgT120I GGNCC 4 cut(s) 121, 159, 296, 354
BmiI GGNNCC 2 cut(s) 160, 298
BmrFI CCNGG 3 cut(s) 119, 156, 352
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 373
BsaJI CCNNGG 1 cut(s) 155
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 363
Bse1I ACTGG 3 cut(s) 124, 264, 368
Bse21I CCTNAGG 1 cut(s) 149
BseBI CCWGG 3 cut(s) 119, 156, 352
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 2 cut(s) 313, 363
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 3 cut(s) 124, 264, 368
BseSI GKGCMC 1 cut(s) 178
BshFI GGCC 3 cut(s) 154, 161, 169
BsiHKAI GWGCWC 1 cut(s) 178
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 313, 363
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 3 cut(s) 154, 161, 169
Bsp1286I GDGCHC 1 cut(s) 178
Bsp1407I TGTACA 1 cut(s) 29
Bsp143I GATC 2 cut(s) 212, 303
BspACI CCGC 2 cut(s) 60, 166
BspANI GGCC 3 cut(s) 154, 161, 169
BspCNI CTCAG 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 160, 298
BspPI GGATC 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 29
BsrI ACTGG 3 cut(s) 124, 264, 368
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 2 cut(s) 212, 303
Bst2UI CCWGG 3 cut(s) 119, 156, 352
BstAUI TGTACA 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 77, 149, 266
BstF5I GGATG 1 cut(s) 349
BstKTI GATC 2 cut(s) 215, 306
BstMBI GATC 2 cut(s) 212, 303
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstNI CCWGG 3 cut(s) 119, 156, 352
BstSCI CCNGG 3 cut(s) 117, 154, 350
BstSLI GKGCMC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 3 cut(s) 154, 161, 169
BtsCI GGATG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 398
Cfr13I GGNCC 4 cut(s) 121, 159, 296, 354
Csp6I GTAC 2 cut(s) 30, 361
CviAII CATG 2 cut(s) 163, 313
CviJI RGCY 8 cut(s) 63, 154, 161, 169, 186, 230, 263, 400
CviKI_1 RGCY 8 cut(s) 63, 154, 161, 169, 186, 230, 263, 400
CviQI GTAC 2 cut(s) 30, 361
DdeI CTNAG 3 cut(s) 77, 149, 266
DpnI GATC 2 cut(s) 214, 305
DpnII GATC 2 cut(s) 212, 303
DraIII CACNNNGTG 1 cut(s) 182
EaeI YGGCCR 1 cut(s) 167
Eco147I AGGCCT 1 cut(s) 154
Eco47I GGWCC 3 cut(s) 121, 296, 354
Eco81I CCTNAGG 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 117, 154, 350
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 166, 316
FaqI GGGAC 1 cut(s) 282
FatI CATG 2 cut(s) 162, 312
Fnu4HI GCNGC 2 cut(s) 61, 167
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 2 cut(s) 61, 167
FspBI CTAG 1 cut(s) 308
GluI GCNGC 2 cut(s) 61, 167
HaeIII GGCC 3 cut(s) 154, 161, 169
Hin1II CATG 2 cut(s) 166, 316
Hpy166II GTNNAC 2 cut(s) 176, 201
Hpy188I TCNGA 3 cut(s) 252, 267, 288
Hpy8I GTNNAC 2 cut(s) 176, 201
HpyCH4V TGCA 2 cut(s) 4, 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 3 cut(s) 77, 149, 266
Hsp92II CATG 2 cut(s) 166, 316
Kzo9I GATC 2 cut(s) 212, 303
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 17
MalI GATC 2 cut(s) 214, 305
MboI GATC 2 cut(s) 212, 303
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 3 cut(s) 48, 191, 275
MnlI CCTC 3 cut(s) 81, 358, 367
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 47
MslI CAYNNNNRTG 1 cut(s) 317
MspR9I CCNGG 3 cut(s) 119, 156, 352
MvaI CCWGG 3 cut(s) 119, 156, 352
MwoI GCNNNNNNNGC 1 cut(s) 402
NdeII GATC 2 cut(s) 212, 303
NlaIII CATG 2 cut(s) 166, 316
NlaIV GGNNCC 2 cut(s) 160, 298
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 154
PfoI TCCNGGA 1 cut(s) 350
PkrI GCNGC 2 cut(s) 62, 168
PshBI ATTAAT 1 cut(s) 47
PsiI TTATAA 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 117, 154, 350
PspGI CCWGG 3 cut(s) 117, 154, 350
PspN4I GGNNCC 2 cut(s) 160, 298
PspPI GGNCC 4 cut(s) 121, 159, 296, 354
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 2 cut(s) 31, 362
RsaNI GTAC 2 cut(s) 30, 361
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 2 cut(s) 61, 167
Sau3AI GATC 2 cut(s) 212, 303
Sau96I GGNCC 4 cut(s) 121, 159, 296, 354
ScrFI CCNGG 3 cut(s) 119, 156, 352
SduI GDGCHC 1 cut(s) 178
SetI ASST 6 cut(s) 26, 92, 120, 331, 359, 402
SfaNI GCATC 1 cut(s) 334
SinI GGWCC 3 cut(s) 121, 296, 354
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 3 cut(s) 48, 191, 275
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 2 cut(s) 60, 166
SspMI CTAG 1 cut(s) 308
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 3 cut(s) 117, 154, 350
TasI AATT 3 cut(s) 48, 191, 275
TatI WGTACW 1 cut(s) 29
TauI GCSGC 2 cut(s) 63, 169
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TspDTI ATGAA 2 cut(s) 236, 271
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 3 cut(s) 121, 296, 354
VspI ATTAAT 1 cut(s) 47
XspI CTAG 1 cut(s) 308
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.