Rroxscaffold_3G00229050

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006640
Physical Location & Seq
Reverse (-)
13582516 .. 13584233
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229050.1

Sequence Viewer

Length: 651 bp
ATGACGTTTGTTTATGGCGATTTTTACCCATACGATGTTGAAAAGCAGGAATGGAAGTTAATTTCAAGCCCTAATAGTCCACCCCCTCGTAGCGCTCAACAGGCTGTCACCTGGAAGAACTATGTTTACATCTTCGGTGGTGAGTTTACTTCCCCAAACCAAGAGAGGTTCCATCACTATAAGGACTTTTGGATGTTGGATCTCAAAACAAATCAATGGGAACAGCTAAACTTAAAAGGGAGTCCAAGTCCACGCTCAGGTCATCGAATGGTATTGTACAAGCACAAGATCATTGCTTTTGGCGGCTTCTATGACAATCTAAGAGAAGTGAGGTATCATAATGATCTTTATGTATTTGACCTGGACCAGTTCAAGTGGCAAGAAATAACCCCTAGGCCTGGGGCCGGCCATGCGGCCAAGTGCACGAAGTGGCTTTCAATTTTTGTTTACCAAGATGAGATCTTTGTGTATGGTGGCTATTCCAAAGAAGTTTCATCTGATAAAACCGGCTCTGAGAAAGGAATTGTTCATTCAGATTTATGGTCCCTTGATCCTAGACATGGGAATGGAACAAGGTCTGGGTTTCTTAATGGTTCCTCCTATGAATTGCGATTTGGAAGAAATGAAAAGCACTTTCGTTTTGTTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

216

Amino Acids

25.39

Weight (kDa)

8.78

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 4 - 163 1.5e-29 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 7 - 185 2e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 7 - 78 1.4e-11 Attractin/LZTR1 beta-propeller
NANM PF24996 8 - 130 4.3e-10 N-acetylneuraminate epimerase
Kelch_FKB95 PF25210 15 - 168 5.7e-10 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 60 - 133 3e-14 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 84 - 131 1.7e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 303, 413
AclWI GGATC 2 cut(s) 207, 545
AcoI YGGCCR 2 cut(s) 406, 414
AdeI CACNNNGTG 1 cut(s) 429
AfaI GTAC 1 cut(s) 278
AfeI AGCGCT 1 cut(s) 94
AfiI CCNNNNNNNGG 4 cut(s) 257, 398, 404, 560
AgsI TTSAA 4 cut(s) 41, 66, 373, 438
AjnI CCWGG 3 cut(s) 110, 360, 397
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw21I GWGCWC 1 cut(s) 425
Alw44I GTGCAC 1 cut(s) 421
AlwI GGATC 2 cut(s) 207, 545
Aor51HI AGCGCT 1 cut(s) 94
AoxI GGCC 4 cut(s) 395, 402, 406, 414
ApaLI GTGCAC 1 cut(s) 421
AspA2I CCTAGG 1 cut(s) 392
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 364, 402, 543
AsuHPI GGTGA 2 cut(s) 100, 152
AvaII GGWCC 2 cut(s) 364, 543
AvrII CCTAGG 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 425
BccI CCATC 1 cut(s) 180
BciT130I CCWGG 3 cut(s) 112, 362, 399
BfaI CTAG 3 cut(s) 393, 555, 649
BfoI RGCGCY 1 cut(s) 96
BglII AGATCT 1 cut(s) 459
BisI GCNGC 2 cut(s) 304, 414
BlnI CCTAGG 1 cut(s) 392
BlsI GCNGC 2 cut(s) 305, 415
Bme1390I CCNGG 3 cut(s) 112, 362, 399
Bme18I GGWCC 2 cut(s) 364, 543
BmgT120I GGNCC 3 cut(s) 364, 402, 543
BmiI GGNNCC 4 cut(s) 170, 403, 545, 595
BmrFI CCNGG 3 cut(s) 112, 362, 399
Bpu10I CCTNAGC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 392, 398
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 398, 404, 560
Bse118I RCCGGY 2 cut(s) 404, 506
Bse1I ACTGG 1 cut(s) 367
Bse3DI GCAATG 1 cut(s) 291
BseBI CCWGG 3 cut(s) 112, 362, 399
BseDI CCNNGG 2 cut(s) 392, 398
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 4 cut(s) 257, 398, 404, 560
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 270, 504
BseNI ACTGG 1 cut(s) 367
BseSI GKGCMC 1 cut(s) 425
BshFI GGCC 4 cut(s) 397, 404, 408, 416
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 2 cut(s) 405, 507
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 4 cut(s) 257, 398, 404, 560
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 4 cut(s) 397, 404, 408, 416
Bsp1286I GDGCHC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 5 cut(s) 199, 288, 343, 459, 550
BspACI CCGC 2 cut(s) 303, 413
BspANI GGCC 4 cut(s) 397, 404, 408, 416
BspCNI CTCAG 2 cut(s) 269, 505
BspLI GGNNCC 4 cut(s) 170, 403, 545, 595
BspPI GGATC 2 cut(s) 207, 545
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 2 cut(s) 404, 506
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 2 cut(s) 404, 506
BssECI CCNNGG 2 cut(s) 392, 398
BssMI GATC 5 cut(s) 199, 288, 343, 459, 550
BssT1I CCWWGG 1 cut(s) 392
Bst2UI CCWGG 3 cut(s) 112, 362, 399
BstAUI TGTACA 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 3 cut(s) 256, 320, 513
BstF5I GGATG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 202, 291, 346, 462, 553
BstMBI GATC 5 cut(s) 199, 288, 343, 459, 550
BstMWI GCNNNNNNNGC 2 cut(s) 101, 410
BstNI CCWGG 3 cut(s) 112, 362, 399
BstSCI CCNGG 3 cut(s) 110, 360, 397
BstSLI GKGCMC 1 cut(s) 425
BstX2I RGATCY 2 cut(s) 199, 459
BstYI RGATCY 2 cut(s) 199, 459
BsuRI GGCC 4 cut(s) 397, 404, 408, 416
BtsCI GGATG 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 406
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 2 cut(s) 404, 506
Cfr13I GGNCC 3 cut(s) 364, 402, 543
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 410, 560
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 256, 320, 513
DpnI GATC 5 cut(s) 201, 290, 345, 461, 552
DpnII GATC 5 cut(s) 199, 288, 343, 459, 550
DraIII CACNNNGTG 1 cut(s) 429
EaeI YGGCCR 2 cut(s) 406, 414
Eco130I CCWWGG 1 cut(s) 392
Eco147I AGGCCT 1 cut(s) 397
Eco47I GGWCC 2 cut(s) 364, 543
Eco47III AGCGCT 1 cut(s) 94
EcoRII CCWGG 3 cut(s) 110, 360, 397
EcoT14I CCWWGG 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 2 cut(s) 413, 563
FaqI GGGAC 1 cut(s) 529
FatI CATG 2 cut(s) 409, 559
Fnu4HI GCNGC 2 cut(s) 304, 414
FokI GGATG 1 cut(s) 205
FseI GGCCGGCC 1 cut(s) 408
Fsp4HI GCNGC 2 cut(s) 304, 414
FspBI CTAG 3 cut(s) 393, 555, 649
GlaI GCGC 1 cut(s) 94
GluI GCNGC 2 cut(s) 304, 414
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 397, 404, 408, 416
HapII CCGG 2 cut(s) 405, 507
HhaI GCGC 1 cut(s) 95
Hin1II CATG 2 cut(s) 413, 563
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 1 cut(s) 241
HpaII CCGG 2 cut(s) 405, 507
HphI GGTGA 2 cut(s) 100, 152
Hpy166II GTNNAC 6 cut(s) 80, 127, 147, 251, 423, 448
Hpy188I TCNGA 3 cut(s) 499, 514, 535
Hpy8I GTNNAC 6 cut(s) 80, 127, 147, 251, 423, 448
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 1 cut(s) 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 410
HpyF3I CTNAG 3 cut(s) 256, 320, 513
HpySE526I ACGT 1 cut(s) 5
Hsp92II CATG 2 cut(s) 413, 563
HspAI GCGC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Kzo9I GATC 5 cut(s) 199, 288, 343, 459, 550
MaeI CTAG 3 cut(s) 393, 555, 649
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 106, 643
MalI GATC 5 cut(s) 201, 290, 345, 461, 552
MboI GATC 5 cut(s) 199, 288, 343, 459, 550
MboII GAAGA 3 cut(s) 124, 127, 630
MflI RGATCY 2 cut(s) 199, 459
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 4 cut(s) 60, 438, 522, 605
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 4 cut(s) 96, 159, 324, 607
MroNI GCCGGC 1 cut(s) 404
MseI TTAA 3 cut(s) 59, 233, 588
MslI CAYNNNNRTG 1 cut(s) 564
MspI CCGG 2 cut(s) 405, 507
MspR9I CCNGG 3 cut(s) 112, 362, 399
MvaI CCWGG 3 cut(s) 112, 362, 399
MwoI GCNNNNNNNGC 2 cut(s) 101, 410
NaeI GCCGGC 1 cut(s) 406
NdeII GATC 5 cut(s) 199, 288, 343, 459, 550
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 2 cut(s) 413, 563
NlaIV GGNNCC 4 cut(s) 170, 403, 545, 595
NmuCI GTSAC 1 cut(s) 106
PceI AGGCCT 1 cut(s) 397
PdiI GCCGGC 1 cut(s) 406
PkrI GCNGC 2 cut(s) 305, 415
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
Psp6I CCWGG 3 cut(s) 110, 360, 397
PspGI CCWGG 3 cut(s) 110, 360, 397
PspN4I GGNNCC 4 cut(s) 170, 403, 545, 595
PspPI GGNCC 3 cut(s) 364, 402, 543
PsrI GAACNNNNNNTAC 2 cut(s) 110, 142
PsuI RGATCY 2 cut(s) 199, 459
RigI GGCCGGCC 1 cut(s) 408
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 3 cut(s) 59, 233, 588
SatI GCNGC 2 cut(s) 304, 414
Sau3AI GATC 5 cut(s) 199, 288, 343, 459, 550
Sau96I GGNCC 3 cut(s) 364, 402, 543
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 3 cut(s) 112, 362, 399
SduI GDGCHC 1 cut(s) 425
SetI ASST 9 cut(s) 8, 113, 170, 228, 262, 335, 363, 578, 650
SinI GGWCC 2 cut(s) 364, 543
SmiMI CAYNNNNRTG 1 cut(s) 564
Sse9I AATT 4 cut(s) 60, 438, 522, 605
SseBI AGGCCT 1 cut(s) 397
SsiI CCGC 2 cut(s) 303, 413
SspMI CTAG 3 cut(s) 393, 555, 649
StuI AGGCCT 1 cut(s) 397
StyD4I CCNGG 3 cut(s) 110, 360, 397
StyI CCWWGG 1 cut(s) 392
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 265
TasI AATT 4 cut(s) 60, 438, 522, 605
TatI WGTACW 1 cut(s) 276
TauI GCSGC 2 cut(s) 306, 416
Tru1I TTAA 3 cut(s) 59, 233, 588
Tru9I TTAA 3 cut(s) 59, 233, 588
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 4 cut(s) 483, 518, 618, 639
VneI GTGCAC 1 cut(s) 421
VpaK11BI GGWCC 2 cut(s) 364, 543
XmaJI CCTAGG 1 cut(s) 392
XspI CTAG 3 cut(s) 393, 555, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.