RchiOBHm_Chr6g0266051

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006640
Physical Location & Seq
Reverse (-)
22140344 .. 22142216
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23860

Sequence Viewer

Length: 498 bp
ATGTGTTCGTGTGTGTTGAGACACACACTTTACAACATTAGTCCAGTCTTCATCTCTGTTCATTGTTTTTTTACAGTGATGCATTATCAGTTATGTTACAGGTATTGTACAAGCACAAGATTATTAATTGTTTTTGGCGGCTTCTATGACACTCTAAGAGAAGTGAGGTATCATAATGATCTTTATGTATTTGACCTGGACCAGTTCAAGTGGCAAGAAATAACCCTTAGGCCTGGGGCCATGCGGCCAAGTGCACGAAGTGGCTTTCAATTTTTGTTTACCAAGATGAGATCTTTCTGTATGGTGGCTATTCCAAAGAAGTTTCATCTGATAAAACTGGCTCTGAGAAAGGAATTGTTCATTCAGATTTATGGTCCCTTGATCCTAGACATGGGAATGGAACAAGGTAAGAAAAAGTGGGATGCCTCTTGGACCTCGTACTGGGTTTTCTATATATGTGTGTTTATAAGAAGCGAGCTTTGCTATTTGGGGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

165

Amino Acids

19.75

Weight (kDa)

9.24

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 40 - 91 1.2e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AciI CCGC 2 cut(s) 138, 244
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 245
AdeI CACNNNGTG 1 cut(s) 260
AfaI GTAC 2 cut(s) 109, 440
AfiI CCNNNNNNNGG 2 cut(s) 391, 441
AgsI TTSAA 2 cut(s) 208, 269
AjnI CCWGG 2 cut(s) 195, 232
AluBI AGCT 1 cut(s) 478
AluI AGCT 1 cut(s) 478
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 1 cut(s) 13
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 376
AoxI GGCC 3 cut(s) 230, 237, 245
ApaLI GTGCAC 1 cut(s) 252
AseI ATTAAT 1 cut(s) 125
AspS9I GGNCC 4 cut(s) 199, 237, 374, 432
AvaII GGWCC 3 cut(s) 199, 374, 432
AxyI CCTNAGG 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 256
BciT130I CCWGG 2 cut(s) 197, 234
BcoDI GTCTC 1 cut(s) 13
BfaI CTAG 1 cut(s) 386
BglII AGATCT 1 cut(s) 290
BisI GCNGC 2 cut(s) 139, 245
BlsI GCNGC 2 cut(s) 140, 246
Bme1390I CCNGG 2 cut(s) 197, 234
Bme18I GGWCC 3 cut(s) 199, 374, 432
BmgT120I GGNCC 4 cut(s) 199, 237, 374, 432
BmiI GGNNCC 2 cut(s) 238, 376
BmrFI CCNGG 2 cut(s) 197, 234
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 69, 412
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 391, 441
Bse1I ACTGG 4 cut(s) 44, 202, 342, 446
Bse21I CCTNAGG 1 cut(s) 227
BseBI CCWGG 2 cut(s) 197, 234
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 391, 441
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 4 cut(s) 44, 202, 342, 446
BseSI GKGCMC 1 cut(s) 256
BshFI GGCC 3 cut(s) 232, 239, 247
BsiHKAI GWGCWC 1 cut(s) 256
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 2 cut(s) 391, 441
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 3 cut(s) 232, 239, 247
Bsp1286I GDGCHC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 107
Bsp143I GATC 3 cut(s) 178, 290, 381
BspACI CCGC 2 cut(s) 138, 244
BspANI GGCC 3 cut(s) 232, 239, 247
BspCNI CTCAG 1 cut(s) 336
BspLI GGNNCC 2 cut(s) 238, 376
BspPI GGATC 1 cut(s) 376
BsrGI TGTACA 1 cut(s) 107
BsrI ACTGG 4 cut(s) 44, 202, 342, 446
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 3 cut(s) 178, 290, 381
Bst2UI CCWGG 2 cut(s) 197, 234
Bst4CI ACNGT 1 cut(s) 76
BstAUI TGTACA 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 3 cut(s) 155, 227, 344
BstF5I GGATG 1 cut(s) 427
BstKTI GATC 3 cut(s) 181, 293, 384
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 3 cut(s) 178, 290, 381
BstMWI GCNNNNNNNGC 1 cut(s) 480
BstNI CCWGG 2 cut(s) 197, 234
BstSCI CCNGG 2 cut(s) 195, 232
BstSLI GKGCMC 1 cut(s) 256
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 3 cut(s) 232, 239, 247
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 476
Cfr13I GGNCC 4 cut(s) 199, 237, 374, 432
Csp6I GTAC 2 cut(s) 108, 439
CviAII CATG 2 cut(s) 241, 391
CviJI RGCY 8 cut(s) 141, 232, 239, 247, 264, 308, 341, 478
CviKI_1 RGCY 8 cut(s) 141, 232, 239, 247, 264, 308, 341, 478
CviQI GTAC 2 cut(s) 108, 439
DdeI CTNAG 3 cut(s) 155, 227, 344
DpnI GATC 3 cut(s) 180, 292, 383
DpnII GATC 3 cut(s) 178, 290, 381
DraIII CACNNNGTG 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 245
Eco147I AGGCCT 1 cut(s) 232
Eco47I GGWCC 3 cut(s) 199, 374, 432
Eco81I CCTNAGG 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 195, 232
EcoT22I ATGCAT 1 cut(s) 84
FaeI CATG 2 cut(s) 244, 394
FaqI GGGAC 1 cut(s) 360
FatI CATG 2 cut(s) 240, 390
Fnu4HI GCNGC 2 cut(s) 139, 245
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 2 cut(s) 139, 245
FspBI CTAG 1 cut(s) 386
GluI GCNGC 2 cut(s) 139, 245
HaeIII GGCC 3 cut(s) 232, 239, 247
Hin1II CATG 2 cut(s) 244, 394
Hpy166II GTNNAC 2 cut(s) 254, 279
Hpy188I TCNGA 3 cut(s) 330, 345, 366
Hpy8I GTNNAC 2 cut(s) 254, 279
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 82, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 480
HpyF3I CTNAG 3 cut(s) 155, 227, 344
Hsp92II CATG 2 cut(s) 244, 394
Kzo9I GATC 3 cut(s) 178, 290, 381
LpnPI CCDG 9 cut(s) 57, 85, 182, 209, 215, 219, 246, 323, 427
LweI GCATC 2 cut(s) 69, 412
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 180, 292, 383
MboI GATC 3 cut(s) 178, 290, 381
MboII GAAGA 1 cut(s) 40
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 3 cut(s) 126, 269, 353
MnlI CCTC 3 cut(s) 159, 436, 445
Mph1103I ATGCAT 1 cut(s) 84
MseI TTAA 1 cut(s) 125
MslI CAYNNNNRTG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 197, 234
MvaI CCWGG 2 cut(s) 197, 234
MwoI GCNNNNNNNGC 1 cut(s) 480
NdeII GATC 3 cut(s) 178, 290, 381
NlaIII CATG 2 cut(s) 244, 394
NlaIV GGNNCC 2 cut(s) 238, 376
NsiI ATGCAT 1 cut(s) 84
PceI AGGCCT 1 cut(s) 232
PkrI GCNGC 2 cut(s) 140, 246
PshBI ATTAAT 1 cut(s) 125
PsiI TTATAA 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 195, 232
PspGI CCWGG 2 cut(s) 195, 232
PspN4I GGNNCC 2 cut(s) 238, 376
PspPI GGNCC 4 cut(s) 199, 237, 374, 432
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 2 cut(s) 109, 440
RsaNI GTAC 2 cut(s) 108, 439
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 2 cut(s) 139, 245
Sau3AI GATC 3 cut(s) 178, 290, 381
Sau96I GGNCC 4 cut(s) 199, 237, 374, 432
ScrFI CCNGG 2 cut(s) 197, 234
SduI GDGCHC 1 cut(s) 256
SetI ASST 6 cut(s) 104, 170, 198, 409, 437, 480
SfaNI GCATC 2 cut(s) 69, 412
SinI GGWCC 3 cut(s) 199, 374, 432
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 3 cut(s) 126, 269, 353
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 2 cut(s) 138, 244
SspMI CTAG 1 cut(s) 386
StuI AGGCCT 1 cut(s) 232
StyD4I CCNGG 2 cut(s) 195, 232
TaaI ACNGT 1 cut(s) 76
TasI AATT 3 cut(s) 126, 269, 353
TatI WGTACW 1 cut(s) 107
TauI GCSGC 2 cut(s) 141, 247
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 81
TspDTI ATGAA 4 cut(s) 40, 50, 314, 349
TspRI CASTG 1 cut(s) 81
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 3 cut(s) 199, 374, 432
VspI ATTAAT 1 cut(s) 125
XspI CTAG 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.